Compound composition with anti-allergic, soothing and repairing effects, preparation method and application

Abstract

The present invention relates to the field of cosmetic technology, and specifically discloses a compound composition with anti-allergic, soothing and repairing effects, as well as a preparation method and application. The preparation method of the compound composition with anti-allergic, soothing and repairing effects comprises the following steps: step 1), mixing peony root, golden chamomile, sophora japonica bud, mussel and ethanol aqueous solution, heating and extracting to obtain a pre-extract; step 2), adding composite bacteria to the extract, fermenting, filtering, and sterilizing to obtain an extract; step 3), heating the extract to remove ethanol, and obtaining a compound composition with anti-allergic, soothing and repairing effects; the composite bacteria is a combination of Weil's yeast, Lactococcus lactis subsp. cremoris, and Hansenula spores of grape juice. The present invention has the advantage of improving the anti-allergic, soothing and repairing effects.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a compound composition with anti-allergic, soothing and repairing effects, a preparation method and an application thereof. Background Art

[0002] Studies have found that in inflammatory skin cells, the expression levels of inflammatory factors and hyaluronidase are often detected to be elevated; hyaluronic acid (HA) in the intercellular matrix plays a major role in maintaining skin moisture and elasticity, wound healing and angiogenesis, allergic reactions, etc. Excessive expression of hyaluronidase can reduce the amount of hyaluronic acid in the matrix, damage the skin barrier, and also transmit signals, indirectly promoting the expression of inflammatory factors; the main idea used in cosmetics for the treatment or relief of skin allergies is to add anti-allergic ingredients to cosmetics, thereby inhibiting the expression of cellular inflammatory factors, inhibiting the activity of hyaluronidase, and repairing the skin barrier.

[0003] However, existing cosmetics for treating or relieving skin allergies are still relatively general in terms of inhibiting the expression of cellular inflammatory factors, inhibiting the activity of hyaluronidase, and repairing the skin barrier. This results in a slow onset of anti-allergic and soothing repair effects, causing consumers to endure skin discomfort for a longer period of time, making it difficult to meet consumer needs. Therefore, there is still room for improvement. Summary of the invention

[0004] In order to improve the anti-allergic, soothing and repairing effects, the present application provides a compound composition with anti-allergic, soothing and repairing effects, a preparation method and an application thereof.

[0005] In the first aspect, the present application provides a method for preparing a compound composition having anti-allergic, soothing and repairing effects, using the following technical scheme:

[0006] A method for preparing a compound composition having anti-allergic, soothing and repairing effects, comprising the following steps:

[0007] Step 1), mixing peony root, golden chamomile, Sophora japonica bud, mussel and ethanol aqueous solution, heating and extracting to obtain a pre-extract;

[0008] Step 2), adding composite bacteria to the extract, fermenting, filtering, and sterilizing to obtain an extract;

[0009] Step 3), heating the extract to remove ethanol, thereby obtaining a compound composition with anti-allergic, soothing and repairing effects;

[0010] The composite bacteria is a combination of Weill's yeast, Lactococcus lactis subsp. cremoris and Hansenula vitis spores.

[0011] By adopting the above technical scheme, through the composite extraction of peony root, golden chamomile, sophora japonica bud and mussel and the composite fermentation of Weill Saccharomyces cerevisiae, Lactococcus lactis subsp. cremoris and Hansenula vitis spores, the various active ingredients in the compound composition with anti-allergic, soothing and repairing effects can exert a good synergistic effect, can have a very significant effect of inhibiting hyaluronidase and inflammatory factors, can have a rapid anti-allergic and soothing effect, and can also promote the formation of the stratum corneum, quickly increase the thickness of the stratum corneum, and quickly form a skin barrier, so that the skin can be better protected and not easily damaged again, thereby achieving a rapid anti-allergic, soothing and repairing effect on the skin.

[0012] Preferably, in step 1), the mass ratio of peony root, golden chamomile, sophora japonica bud and mussel is 1-5:1-5:0.1-2:0.001-0.01.

[0013] By adopting the above technical scheme and specifically selecting the mass ratio of peony root, golden chamomile, locust flower bud and mussel, the active ingredients in the compound composition with anti-allergic, soothing and repairing effects are combined in a specific ratio, which can better exert the synergistic effect, so that the rapid anti-allergic and soothing repair effect of the compound composition with anti-allergic, soothing and repairing effects is better.

[0014] Preferably, in step 1), the mass concentration of the ethanol aqueous solution is 40-60%.

[0015] By adopting the above technical scheme and specifically selecting the mass concentration of the ethanol aqueous solution, the extraction efficiency is high, and the active ingredients in peony root, golden chamomile, locust flower buds, and mussels can be better extracted into the solvent, so that the various active ingredients in the compound composition with anti-allergic, soothing and repairing effects can better exert a synergistic effect, and the anti-allergic, soothing and repairing effects are better.

[0016] Preferably, in step 1), the mass ratio of peony root, golden chamomile, sophora japonica bud, mussel and ethanol aqueous solution is 1-5:1-5:0.1-2:0.001-0.01:10-60.

[0017] By adopting the above technical scheme and specifically selecting the mass ratio of peony root, golden chamomile, locust flower bud, mussel and ethanol aqueous solution, the active ingredients in peony root, golden chamomile, locust flower bud and mussel can be more fully extracted, while avoiding the waste of solvent, and having higher economic value.

[0018] Preferably, in the step 1), the peony root, golden chamomile, locust flower buds and mussels are first dried and crushed into 20-60 mesh particles, and then mixed with the ethanol aqueous solution.

[0019] By adopting the above technical scheme, through drying and crushing, the active ingredients in peony root, golden chamomile, locust flower buds and mussels are easier to extract, the extraction effect is better, and the prepared compound composition with anti-allergic, soothing and repairing effects is of better quality and better effect.

[0020] Preferably, in step 1), during the heating extraction, the extraction temperature is 60-70° C. and the extraction time is 2-3 hours.

[0021] By adopting the above technical solution and controlling the extraction temperature and extraction time, the extraction effect is improved, while the waste of process time is reduced, which has higher economic value.

[0022] Preferably, in the step 2), the mass ratio of Weil's yeast, Lactococcus lactis subsp. cremoris, and Hansenula vitis spores in the composite bacteria is 0.02-0.03:0.06-0.07:0.12-0.13.

[0023] By adopting the above technical scheme and specifically selecting the mass ratio of Weill's yeast, Lactococcus lactis subsp. cremoris, and Hansenula vitis spores, the fermentation effect is better, and the fermentation product and the active ingredients are combined in a specific ratio to better exert the synergistic effect, and the efficacy of the compound composition with anti-allergic, soothing and repairing effects is more significant.

[0024] Preferably, in step 2), the fermentation temperature is 28-30° C. and the fermentation time is 6-7 days.

[0025] By adopting the above technical scheme and specifically selecting the fermentation temperature, the activity of the composite bacteria is made higher, and by specifically selecting the fermentation time, the fermentation is made more complete and the content of the fermentation product is more appropriate. The obtained composite composition with anti-allergic, soothing and repairing effects is of better quality and has better effects.

[0026] In the second aspect, the present application provides a compound composition with anti-allergic, soothing and repairing effects, which adopts the following technical solution:

[0027] A compound composition with anti-allergic, soothing and repairing effects is prepared by the above-mentioned method for preparing the compound composition with anti-allergic, soothing and repairing effects.

[0028] By adopting the above technical scheme, the compound composition with anti-allergic, soothing and repairing effects can quickly play an anti-allergic, soothing and repairing effect, so that allergic symptoms and discomfort such as skin redness, itching, dryness, tingling, burning, tightness, etc. can be quickly relieved, thus meeting the needs of consumers well.

[0029] In a third aspect, the present application provides an application of a compound composition having anti-allergic, soothing and repairing effects, using the following technical solution:

[0030] An application of the above-mentioned compound composition with anti-allergic, soothing and repairing effects, wherein the compound composition with anti-allergic, soothing and repairing effects is added to cosmetics to prepare cosmetics with anti-allergic, soothing and repairing effects, and the mass content of the compound composition with anti-allergic, soothing and repairing effects in the cosmetics is 0.01-5%.

[0031] By adopting the above technical solution, by adding 0.01-5% of the compound composition with anti-allergic, soothing and repairing effects into cosmetics, the cosmetics can have very significant anti-allergic, soothing and repairing effects, can quickly repair the skin, and make the cosmetics can well meet the needs of consumers.

[0032] In summary, this application has the following beneficial effects:

[0033] 1. Since the present application is extracted through the compound extraction of peony root, golden chamomile, sophora japonica bud, and mussel and fermented through the compound fermentation of Weill Saccharomyces cerevisiae, Lactococcus lactis subsp. cremoris, and Hansenula vitis spores, the various active ingredients in the compound composition with anti-allergic, soothing and repairing effects can play a good synergistic effect, can play a very significant effect of inhibiting hyaluronidase and inflammatory factors, can play a rapid anti-allergic and soothing effect, and can also promote the formation of the stratum corneum, quickly increase the thickness of the stratum corneum, and quickly form a skin barrier, so that the skin can be better protected and not easily damaged again, thereby achieving the effect of rapid anti-allergic, soothing and repairing the skin.

[0034] 2. In the present application, it is preferred to specifically select the mass ratio of peony root, golden chamomile, sophora japonica bud and mussel so that the active ingredients in the compound composition with anti-allergic, soothing and repairing effects are combined in a specific ratio, which can better exert the synergistic effect, so that the compound composition with anti-allergic, soothing and repairing effects has a better effect of rapid anti-allergic, soothing and repairing.

[0035] 3. In the present application, it is preferred to specifically select the mass ratio of Weill's yeast, Lactococcus lactis subsp. cremoris, and Hansenula vitis spores to achieve a better fermentation effect, and the fermentation product and the active ingredient are combined in a specific ratio to better exert the synergistic effect, so that the efficacy of the compound composition with anti-allergic, soothing and repairing effects is more significant. DETAILED DESCRIPTION

[0036] The present application is further described in detail below with reference to the embodiments.

[0037] Example 1

[0038] A method for preparing a compound composition having anti-allergic, soothing and repairing effects, comprising the following steps:

[0039] Step 1), 1kg of peony root, 1kg of golden chamomile, 0.1kg of Sophora japonica buds, and 0.001kg of mussels are dried at 110°C for 2h, then crushed by a crusher, sieved to obtain 20-mesh particles, and then all put into a reactor, and then 10kg of 40% ethanol aqueous solution is added to the reactor, the speed is 120r / min, stirred for 10min, mixed evenly, heated to 60°C in a closed state, stirred continuously at 120r / min, reflux extracted for 2h, and a pre-extract is obtained.

[0040] Step 2), add 0.02g of Will yeast, 0.06g of Lactococcus lactis subsp. cremoris, and 0.12g of Hansen yeast of grape juice to the extract, keep the temperature at 28°C, open ferment for 6 days, then filter through a 400-mesh sieve, take the filtrate, filter the filtrate through a 0.22 μm filter membrane for sterilization, and obtain an extract.

[0041] Step 3), heating the extract to 80°C, condensing and reflux until the extract no longer boils to remove ethanol, thereby obtaining a compound composition with anti-allergic, soothing and repairing effects.

[0042] Peony root comes from commercially available, sun-dried Chinese medicinal materials.

[0043] Golden chamomile comes from commercially available, sun-dried Chinese medicinal materials.

[0044] Sophora japonica flower buds come from commercially available, sun-dried Chinese medicinal materials.

[0045] Mussels are sourced from commercially available, sun-dried Chinese medicinal materials.

[0046] Weil yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-52121.

[0047] Lactococcus lactis subsp. cremoris was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-03523.

[0048] Hansenula vitis var. vitis vinifera was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-64526.

[0049] Example 2

[0050] A method for preparing a compound composition having anti-allergic, soothing and repairing effects, comprising the following steps:

[0051] Step 1), 2.5 kg of peony root, 2.5 kg of golden chamomile, 1 kg of Sophora japonica buds, and 0.005 kg of mussels are dried at 110 ° C for 2 hours, then crushed by a crusher, sieved to obtain 40 mesh particles, and then all put into a reactor, and then 35 kg of 50% ethanol aqueous solution is added to the reactor, the speed is 120 r / min, stirred for 10 minutes, mixed evenly, heated to 65 ° C in a closed state, stirred continuously at 120 r / min, reflux extracted for 2.5 hours, and a pre-extract is obtained.

[0052] Step 2), add 0.025g of Will yeast, 0.065g of Lactococcus lactis subsp. cremoris, and 0.125g of Hansen yeast of grape juice to the extract, keep the temperature at 29°C, open ferment for 6.5 days, then filter through a 500-mesh sieve, take the filtrate, filter the filtrate through a 0.22 μm filter membrane for sterilization, and obtain an extract.

[0053] Step 3), heating the extract to 81°C, condensing and reflux until the extract no longer boils to remove ethanol, thereby obtaining a compound composition with anti-allergic, soothing and repairing effects.

[0054] Peony root comes from commercially available, sun-dried Chinese medicinal materials.

[0055] Golden chamomile comes from commercially available, sun-dried Chinese medicinal materials.

[0056] Sophora japonica flower buds come from commercially available, sun-dried Chinese medicinal materials.

[0057] Mussels are sourced from commercially available, sun-dried Chinese medicinal materials.

[0058] Weil yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-52121.

[0059] Lactococcus lactis subsp. cremoris was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-03523.

[0060] Hansenula vitis var. vitis vinifera was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-64526.

[0061] Example 3

[0062] A method for preparing a compound composition having anti-allergic, soothing and repairing effects, comprising the following steps:

[0063] Step 1), 5kg of peony root, 5kg of golden chamomile, 2kg of Sophora japonica buds, and 0.01kg of mussels are dried at 110°C for 2h, then crushed by a crusher, sieved to obtain 60-mesh particles, and then all put into a reactor, and then 60kg of 60% ethanol aqueous solution is added to the reactor, the speed is 120r / min, stirred for 10min, mixed evenly, heated to 70°C in a closed state, stirred continuously at 120r / min, reflux extracted for 3h, and a pre-extract is obtained.

[0064] Step 2), add 0.03g of Will yeast, 0.07g of Lactococcus lactis subsp. cremoris, and 0.13g of Hansen yeast of grape juice to the extract, keep the temperature at 30°C, open ferment for 7 days, then filter through a 600-mesh sieve, take the filtrate, filter the filtrate through a 0.22 μm filter membrane for sterilization, and obtain an extract.

[0065] Step 3), heating the extract to 82°C, condensing and reflux until the extract no longer boils to remove ethanol, thereby obtaining a compound composition with anti-allergic, soothing and repairing effects.

[0066] Peony root comes from commercially available, sun-dried Chinese medicinal materials.

[0067] Golden chamomile comes from commercially available, sun-dried Chinese medicinal materials.

[0068] Sophora japonica flower buds come from commercially available, sun-dried Chinese medicinal materials.

[0069] Mussels are sourced from commercially available, sun-dried Chinese medicinal materials.

[0070] Weil yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-52121.

[0071] Lactococcus lactis subsp. cremoris was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-03523.

[0072] Hansenula vitis var. vitis vinifera was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-64526.

[0073] Comparative Example 1

[0074] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0075] Replace peony root with an equal amount of dandelion root.

[0076] Dandelion root comes from commercially available, sun-dried Chinese medicinal materials.

[0077] Comparative Example 2

[0078] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0079] Replace golden chamomile with an equal amount of rose petals.

[0080] Rose flowers come from commercially available, sun-dried Chinese medicinal materials.

[0081] Comparative Example 3

[0082] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0083] Replace the acacia buds with an equal amount of lavender.

[0084] Lavender comes from commercially available, sun-dried Chinese medicinal materials.

[0085] Comparative Example 4

[0086] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0087] Replace the mussels with an equal amount of saffron.

[0088] Saffron comes from commercially available, sun-dried Chinese medicinal materials.

[0089] Comparative Example 5

[0090] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0091] Use an equal amount of dandelion root to replace peony root; use an equal amount of rose flower to replace golden chamomile; use an equal amount of lavender to replace locust flower buds; use an equal amount of saffron to replace mussels.

[0092] Dandelion root comes from commercially available, sun-dried Chinese medicinal materials.

[0093] Rose flowers come from commercially available, sun-dried Chinese medicinal materials.

[0094] Lavender comes from commercially available, sun-dried Chinese medicinal materials.

[0095] Saffron comes from commercially available, sun-dried Chinese medicinal materials.

[0096] Comparative Example 6

[0097] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0098] Candida glabrata was used to replace the Weil yeast in an equal amount.

[0099] Candida glabrata was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67394.

[0100] Comparative Example 7

[0101] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0102] Bacillus megaterium was used to replace Lactococcus lactis subsp. cremoris in equal amounts.

[0103] Bacillus megaterium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-74912.

[0104] Comparative Example 8

[0105] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0106] An equal amount of Saccharomyces cerevisiae was used to replace Hansenula sporogenes in grape juice.

[0107] Saccharomyces cerevisiae was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-68045.

[0108] Comparative Example 9

[0109] A method for preparing a compound composition with anti-allergic, soothing and repairing effects, compared with Example 2, the only difference is:

[0110] Candida glabrata was used to replace Weil's yeast in equal amounts; Bacillus megaterium was used to replace Lactococcus lactis subsp. cremoris in equal amounts; and Saccharomyces cerevisiae was used to replace Hansenula vitis vinifera in equal amounts.

[0111] Candida glabrata was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67394.

[0112] Bacillus megaterium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-74912.

[0113] Saccharomyces cerevisiae was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-68045.

[0114] Experiment 1

[0115] The hyaluronidase in vitro inhibition experiment was used to detect the inhibitory effect of the test solution on the activity of hyaluronidase, so as to verify the anti-allergic and anti-irritation effects of the present invention.

[0116] For the sample to be tested, 2 g of the compound composition with anti-allergic, soothing and repairing effects and 98 g of deionized water are mixed evenly to obtain a sample to be tested with a concentration of 2.0%.

[0117] Experiment 2

[0118] Inhibition of cellular inflammatory factors

[0119] (1) Cell seeding: dilute macrophages (mouse mononuclear macrophage leukemia cell line RAW264.7, purchased from the Cell Bank of the Chinese Academy of Sciences, catalog number: TCM13) with cell culture medium to the seeding density (the degree of confluence reaches 45%-60% 24 h after seeding), seed them into 96-well plates with 200 μL per well, and place them in a CO2 incubator for 24±2 h.

[0120] (2) Administration: Discard the culture medium in the 96-well plate and carry out induction and administration. Add culture medium containing a certain concentration of the test substance and 1 μg / mL LPS to the test substance (compound composition prepared by the present invention) wells, add cell culture medium containing LPS to the negative control wells, and add cell culture medium to the blank control wells, 200 μL per well, and continue to culture in a CO2 incubator for 24±2 h.

[0121] (3) Collection of cell supernatant: After the incubation, collect 200 μL of cell culture supernatant into a 1.5 mL sterile centrifuge tube and store it in a -80°C ultra-low temperature refrigerator.

[0122] (4) ELISA test: Perform the test according to the operating instructions of the TNF-α ELISA test kit.

[0123] Experiment 3

[0124] A number of female volunteers aged 35-40 were recruited and randomly divided into groups of 10 for the experiment.

[0125] Preparation of test samples:

[0126] 5 g of the compound composition with anti-allergic, soothing and repairing effects and 95 g of deionized water are mixed evenly to obtain a sample to be tested.

[0127] The volunteers used the test sample once in the morning and once in the evening. Before use, they cleaned their face with clean water and a disposable face washcloth, then took 3 ml of the test sample and spread it evenly on the face. They used it continuously for 14 days.

[0128] On the 15th day, the test samples were no longer used, and the face was cleaned with clean water and a disposable face towel. The skin moisture content was tested after 10 minutes, recorded as the skin moisture content after cleansing. Then, the subjects were allowed to move freely for 8 hours, and the skin moisture content was tested again, recorded as the skin moisture content after 8 hours.

[0129] The specific test data of Experiments 1-3 are shown in Table 1.

[0130] Table 1

[0131]

[0132] According to the data comparison of each embodiment and the comparative example in Table 1, the hyaluronidase inhibition rate and TNF-α inhibition rate of each embodiment are higher than those of the comparative examples. It can be seen that the compound composition with anti-allergic, soothing and repairing effects obtained after the compounding of peony root, golden chamomile, sophora japonica bud and mussel and the composite fermentation of Weill's yeast, Lactococcus lactis subsp. cremoris and Hansenula vitis spores can have better anti-allergic and soothing effects. In addition, the skin moisture content of each embodiment after 8 hours is significantly higher than that of each comparative example. It can be seen that the compound composition with anti-allergic, soothing and repairing effects of each embodiment can quickly repair the skin barrier, so that the skin barrier can function normally. The skin barrier repair is mainly reflected in the fact that skin moisture is not easily lost. Due to the repair of the skin barrier, the skin is not easily damaged again, and allergies are not easily recurring, thereby achieving a significant repair effect.

[0133] In Comparative Examples 1-5, peony root, golden chamomile, sophora japonica buds, and mussels were replaced singly or completely by other plants with anti-allergic effects. Since the compounding of peony root, golden chamomile, sophora japonica buds, and mussels was destroyed, the obtained composition also has the effect of inhibiting hyaluronidase and TNF-ɑ inflammatory factors, but the effect is far less than that of the various embodiments, and the effect of repairing the skin barrier has been significantly reduced. It can be seen that after the compounding of peony root, golden chamomile, sophora japonica buds, and mussels, the active ingredients play a special synergistic role, which can play a better anti-allergic, soothing, and repairing effect.

[0134] In Comparative Examples 6-9, Weil's yeast, Lactococcus lactis cremoris, and Hansenula vitis spores were replaced solely or completely by other fermentation bacteria, resulting in changes in the fermentation products, thereby causing a decrease in the hyaluronidase inhibition rate, TNF-ɑ inhibition rate, and skin barrier repair ability. It can be seen that the special fermentation products of Weil's yeast, Lactococcus lactis cremoris, and Hansenula vitis spores cooperate with the active ingredients in peony root, golden chamomile, locust flower buds, and mussels to achieve better anti-allergic, soothing, and repairing effects.

[0135] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed. However, as long as it is within the scope of the claims of the present application, it shall be protected by the patent law.

Claims

1. A method for preparing a compound composition having anti-allergic, soothing and repairing effects, characterized in that: The following steps are involved: Step 1), mixing peony root, golden chamomile, Sophora japonica bud, mussel and ethanol aqueous solution, heating and extracting to obtain a pre-extract; Step 2), adding composite bacteria to the pre-extract, fermenting, filtering, and sterilizing to obtain an extract; Step 3), heating the extract to remove ethanol, thereby obtaining a compound composition with anti-allergic, soothing and repairing effects; The composite bacteria is a combination of Weill's yeast, Lactococcus lactis subsp. cremoris, and Hansenula vitis spores. In the step 1), the mass ratio of peony root, golden chamomile, sophora japonica bud and mussel is 1-5:1-5:0.1-2:0.001-0.01; In the step 1), the mass concentration of the ethanol aqueous solution is 40-60%; In the step 2), the mass ratio of Weil's yeast, Lactococcus lactis subsp. cremoris, and Hansenula vitis spores in the composite bacteria is 0.02-0.03:0.06-0.07:0.12-0.13; In the step 2), the fermentation temperature is 28-30° C. and the fermentation time is 6-7 days.

2. The method for preparing a compound composition having anti-allergic, soothing and repairing effects according to claim 1, characterized in that: In the step 1), the mass ratio of peony root, golden chamomile, sophora japonica bud, mussel and ethanol aqueous solution is 1-5:1-5:0.1-2:0.001-0.01:10-60.

3. The method for preparing a compound composition having anti-allergic, soothing and repairing effects according to claim 2, characterized in that: In the step 1), the peony root, golden chamomile, locust flower buds and mussels are first dried and crushed into 20-60 mesh particles, and then mixed with ethanol aqueous solution.

4. The method for preparing a compound composition having anti-allergic, soothing and repairing effects according to claim 3, characterized in that: In the step 1), during the heating extraction, the extraction temperature is 60-70° C. and the extraction time is 2-3 hours.

5. A compound composition with anti-allergic, soothing and repairing effects, characterized in that: The compound composition with anti-allergic, soothing and repairing effects is prepared by the preparation method of the compound composition with anti-allergic, soothing and repairing effects described in any one of claims 1-4.

6. An application of the compound composition with anti-allergic, soothing and repairing effects according to claim 5, characterized in that: The compound composition with anti-allergic, soothing and repairing effects is added to cosmetics to prepare cosmetics with anti-allergic, soothing and repairing effects, and the mass content of the compound composition with anti-allergic, soothing and repairing effects in the cosmetics is 0.01-5%.

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