A Panax notoginseng root extract and its preparation method and application

Abstract

The present invention provides a Panax notoginseng root extract and a preparation method and application thereof, relating to the technical field of traditional Chinese medicine. The preparation method of the present invention comprises the following steps: (1) steam-exploding the Panax notoginseng root to obtain a steam-exploded product; (2) extracting with a weakly acidic aqueous solution, collecting a filtrate and a filter residue; drying the filtrate to obtain a Panax notoginseng root extract A; (3) mixing the filter residue with a surfactant and an organic solvent, heating and extracting, and then ultrasonically treating the filter residue, and drying the filtrate to obtain a Panax notoginseng root extract B; the surfactant is polyethylene glycol 200 and Tween 80 in a weight ratio of 2-4:1; the volume concentration of ethanol in the organic solvent is 15-30%, and the volume concentration of ethyl acetate is 50-60%; (4) mixing the Panax notoginseng root extract A and the Panax notoginseng root extract B to obtain the Panax notoginseng root extract. The Panax notoginseng root extract of the present invention has significant antioxidant and anti-tumor effects.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a Panax notoginseng root extract, a preparation method and an application thereof. Background Art

[0002] Panax notoginseng, also known as Tianqi (Panax notoginseng) and Jinbuhuan (Golden Notoginseng), has a sweet and slightly bitter flavor and a warm nature. It primarily enters the sweet, stomach, and large intestine meridians, and has nourishing, strengthening, blood-tonifying, analgesic, hemostatic, and immune-enhancing properties. Numerous chemical components have been isolated from Panax notoginseng, primarily including notoginseng saponins (PNS), polysaccharides, flavonoids, sterols, volatile oils, amino acids, and various trace elements.

[0003] Currently, research on the chemical components of Panax notoginseng has mostly focused on its saponins. Panax notoginseng saponins have the effects of promoting blood circulation and removing blood stasis, activating blood vessels, inhibiting platelet aggregation, and increasing cerebral blood flow. They are widely used in medications for the treatment of cerebrovascular sequelae, central retinal vein occlusion, and hyphema. Panax notoginseng saponins and some of its monomeric compounds have excellent pharmacological activity in the cardiovascular system, nervous system, metabolism, and anti-inflammatory and anti-tumor aspects. Panax notoginseng saponins and their monomeric saponins Rb1 and Rg1 can significantly enhance the learning and memory abilities of mice. Panax notoginseng saponins can also reduce the body's oxygen consumption, improve the body's tolerance to hypoxia, inhibit platelet aggregation, dilate cerebral blood vessels, increase cerebral blood flow, and have anti-thrombotic and anti-coagulant effects.

[0004] Different extraction methods for Panax notoginseng yield different saponin compositions and contents, leading to different efficacy outcomes. Chinese invention patent CN116270779A discloses a method for preparing a Panax notoginseng extract, comprising the steps of extracting Panax notoginseng using an extraction solvent comprising, by weight, 10-30 parts aqueous citric acid solution, 1-12 parts propylene glycol, and 0.1-5 parts sodium alginate-immobilized xylanase. This method improves the extraction efficiency of the active ingredients in the Panax notoginseng extract by controlling the composition of the extraction solvent, the contents of the various components in the solvent, and the mass ratio of Panax notoginseng to the extraction solvent. The total saponin content of Panax notoginseng obtained by this method is no less than 84.2%, and the active ingredients include no less than 8.1% ginsenoside R1, no less than 34.6% ginsenoside Rg1, no less than 37.2% ginsenoside Rb1, and no less than 5.5% ginsenoside Rd.

[0005] Chinese invention patent CN108938696A discloses a method for extracting total saponins from Panax notoginseng, including enzymatic treatment, aqueous extraction, macroporous resin adsorption, activated carbon adsorption, and cation exchange resin adsorption. This method adds citric acid as an antioxidant to the aqueous extraction process, which can improve product quality and yield. Extracting Panax notoginseng with water as the solvent can reduce residual organic solvent in the product. Chinese invention patent CN116098288A discloses a Panax notoginseng oral liquid for lowering blood pressure and its preparation method. This invention uses fresh Panax notoginseng fibrous roots as raw material, which are ultrafinely ground, and then the active ingredients, such as Panax notoginseng saponins, are extracted. The extract is then used to prepare the Panax notoginseng oral liquid, the main active ingredient being Panax notoginseng saponins. This oral liquid has significant advantages in resisting hypoxia, aging, hypoxia tolerance, and enhancing human immunity. Compared to using ordinary Panax notoginseng roots (taproots) as raw materials, the ultrafine grinding used in this invention significantly promotes the dissolution of active ingredients, such as Panax notoginseng saponins.

[0006] In addition to notoginsenosides, other chemical components in Panax notoginseng also have different active effects. Chinese invention patent CN102961424A discloses a Panax notoginseng polysaccharide extract, its preparation method, preparation, and application. The Panax notoginseng polysaccharide extract is obtained from the waste residue after extracting the total saponins of Panax notoginseng. The extract is extracted, concentrated, refined, dried, crushed, and tested. The specific steps are: adding the waste residue after extracting the total saponins of Panax notoginseng to purified water, extracting at 50-60°C for 4-8 hours, recycling the extraction for 2-6 times, filtering, concentrating, adding ethanol to precipitate for 8-24 hours, filtering, refining, and drying to obtain the target dry product. The Panax notoginseng polysaccharide extract is used to prepare anti-tumor drugs and immune-enhancing drugs.

[0007] In order to achieve the comprehensive extraction and utilization of the chemical components in Panax notoginseng root hairs, it is necessary to provide a method for efficiently extracting multiple chemical components from Panax notoginseng root hairs, improve the extraction effect of Panax notoginseng root hairs extract, and give full play to the efficacy of Panax notoginseng root hairs extract. Summary of the Invention

[0008] The present invention addresses the problems existing in the prior art and provides a Panax notoginseng root extract, a preparation method, and applications thereof. The Panax notoginseng root extracts are first subjected to steam explosion and then extracted with a weakly acidic aqueous solution to obtain a Panax notoginseng root extract A rich in water-soluble chemical components. The filtered residue is then mixed with a surfactant and an organic solvent, subjected to heating extraction, and then ultrasonically treated to obtain a Panax notoginseng root extract B. The Panax notoginseng root extract A and the Panax notoginseng root extract B are then mixed to obtain a Panax notoginseng root extract. The Panax notoginseng root extract of the present invention significantly enhances antioxidant and cancer cell inhibition effects.

[0009] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0010] First, the present invention provides a method for preparing a Panax notoginseng root extract, comprising the steps of:

[0011] (1) After the Panax notoginseng fibrous roots are soaked in a weakly acidic aqueous solution, they are subjected to steam explosion treatment at a pressure of 0.5-1.2 MPa for 1-3 minutes to obtain a steam-exploded product;

[0012] (2) mixing the steam explosion-treated product with a weakly acidic aqueous solution, extracting, filtering, collecting the filtrate and filter residue; and drying the filtrate to obtain Panax notoginseng root extract A;

[0013] (3) mixing the filter residue from step (2) with a surfactant and an organic solvent, heating and extracting at 40-55° C. for 3-6 hours; ultrasonically treating for 25-60 minutes, filtering, and drying the filtrate to obtain Panax notoginseng root extract B;

[0014] The surfactant is polyethylene glycol 200 (PEG 200) and Tween 80 in a weight ratio of 2-4:1;

[0015] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 15-30%, and the volume concentration of ethyl acetate is 50-60%;

[0016] (4) Mixing the Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) to obtain a Panax notoginseng root extract.

[0017] Preferably, in step (1) and step (2), the weakly acidic aqueous solution is a formic acid aqueous solution with a volume concentration of 0.05-0.2%.

[0018] Further preferably, in step (1) and step (2), the weakly acidic aqueous solution is a formic acid aqueous solution with a volume concentration of 0.1%.

[0019] Preferably, in step (1), the soaking time is 8-24 hours; further preferably, the soaking time is 12 hours.

[0020] Preferably, in step (1), the soaking is to completely submerge the Panax notoginseng roots in a weakly acidic aqueous solution.

[0021] Preferably, in step (1), the steam explosion treatment condition is 1.0 MPa for 2 minutes.

[0022] Preferably, in step (2), the extraction is specifically: stirring and extracting at room temperature for 0.5-2h at a solid-to-liquid ratio of 1:20-30g / mL.

[0023] Further preferably, in step (2), the extraction is specifically: stirring and extracting at room temperature for 1 hour at a solid-to-liquid ratio of 1:25 g / mL.

[0024] Preferably, in step (2), the drying is freeze-drying.

[0025] Preferably, in step (3), the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1.

[0026] Preferably, in step (3), the organic solvent is a mixture of ethanol, ethyl acetate and water; wherein the volume concentration of ethanol is 25%, and the volume concentration of ethyl acetate is 55%.

[0027] Preferably, in step (3), the mass ratio of the surfactant to the organic solvent is 2.5-5:100.

[0028] Further preferably, in step (3), the mass ratio of the surfactant to the organic solvent is 3.5:100.

[0029] Preferably, step (3) is specifically as follows: mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:25-35 g / mL, and extracting under reduced pressure and heating at 45-50°C for 4-5 hours; then performing ultrasonic treatment for 30-50 minutes and filtering, concentrating the filtrate under reduced pressure, removing the organic solvent and then drying to obtain Panax notoginseng root extract B.

[0030] Further preferably, step (3) is specifically as follows: mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting under reduced pressure and heating at 50°C for 4 hours; then performing ultrasonic treatment for 40 minutes and filtering, concentrating the filtrate under reduced pressure, removing the organic solvent and then drying to obtain Panax notoginseng root extract B.

[0031] Then, the present invention provides a Panax notoginseng root extract prepared by the above preparation method.

[0032] Finally, the present invention provides the use of the Panax notoginseng root extract in the preparation of medicines with antioxidant and anti-tumor effects.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] 1. The preparation method of the present invention performs steam explosion treatment on the Panax notoginseng root fibers to further destroy the plant tissue of the Panax notoginseng root fibers, which facilitates the efficient dissolution of chemically active ingredients and realizes efficient extraction of chemically active ingredients from the Panax notoginseng root fibers; weakly acidic water is used for extraction to efficiently extract water-soluble chemically active ingredients.

[0035] 2. The preparation method of the present invention is to subject the filter residue after steam explosion and weak acid water extraction to organic solvent extraction assisted ultrasonic treatment, wherein the organic solvent contains Tween 80 and PEG 200, which are conducive to the dissolution of Panax notoginseng saponins; the assisted ultrasonic treatment further improves the extraction efficiency of the Panax notoginseng root extract and fully exerts the efficacy of the Panax notoginseng root extract.

[0036] 3. The Panax notoginseng root extract obtained by the preparation method of the present invention has multiple chemically active ingredients that complement and enhance each other, and can be used in drugs with antioxidant and anti-tumor properties, with significantly improved efficacy. DETAILED DESCRIPTION

[0037] The following non-limiting examples can make those of ordinary skill in the art understand the present invention more comprehensively, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of protection of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and they should also fall within the scope of protection of the present invention. When the embodiments provide numerical ranges, it should be understood that, unless otherwise specified in the present invention, the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those of ordinary skill in the art to which the present invention belongs.

[0038] The present invention is further described below by way of specific examples. Unless otherwise specified, the various chemical reagents used in the examples of the present invention were obtained through conventional commercial channels. The products of different manufacturers did not significantly affect the effects.

[0039] In the following examples, the polyethylene glycol 200 was purchased from Guangdong Nanhui New Materials Co., Ltd. with the product number of polyethylene glycol PEG200DL; the Tween 80 was purchased from Guangdong Nanhui New Materials Co., Ltd. with the product number of Tween T-80.

[0040] Example 1

[0041] A preparation method of Panax notoginseng root extract is as follows:

[0042] (1) Cutting Panax notoginseng root segments (1-2 cm) into sections, mixing with a 0.1% formic acid aqueous solution, soaking for 12 h, and then steam-exploding the sections at 1.0 MPa for 2 min to obtain steam-exploded products;

[0043] (2) The steam explosion-treated product was mixed with a 0.1% by volume formic acid aqueous solution at a solid-liquid ratio of 1:25 g / mL, stirred and extracted at room temperature for 1 hour, filtered, and the filtrate and residue were collected; the filtrate was concentrated and freeze-dried to obtain Panax notoginseng root extract A;

[0044] (3) The filter residue from step (2) was mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating at 50°C under reduced pressure for 4 h; then ultrasonically treated for 40 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0045] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0046] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0047] (4) Mixing the Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) to obtain a Panax notoginseng root extract.

[0048] Example 2

[0049] A preparation method of Panax notoginseng root extract is as follows:

[0050] (1) Cutting Panax notoginseng root segments (1-2 cm) into sections, mixing them with a 0.05% formic acid aqueous solution, soaking them for 12 h, and then steam-exploding them at 0.5 MPa for 3 min to obtain steam-exploded products;

[0051] (2) The steam explosion-treated product was mixed with a 0.05% by volume formic acid aqueous solution at a solid-liquid ratio of 1:25 g / mL, stirred and extracted at room temperature for 1 hour, filtered, and the filtrate and residue were collected; the filtrate was concentrated and freeze-dried to obtain Panax notoginseng root extract A;

[0052] (3) The filter residue from step (2) was mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating under reduced pressure at 45°C for 5 h; then ultrasonically treated for 50 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0053] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 2:1; the mass ratio of the surfactant to the organic solvent is 2.5:100;

[0054] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 15% and the volume concentration of ethyl acetate is 60%;

[0055] (4) Mixing the Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) to obtain a Panax notoginseng root extract.

[0056] Example 3

[0057] A preparation method of Panax notoginseng root extract is as follows:

[0058] (1) Cutting Panax notoginseng root segments (1-2 cm) into sections, mixing them with a 0.2% formic acid aqueous solution, soaking them for 12 h, and then steam-exploding them at 1.2 MPa for 1 min to obtain steam-exploded products;

[0059] (2) The steam explosion-treated product was mixed with a 0.2% by volume formic acid aqueous solution at a solid-liquid ratio of 1:25 g / mL, stirred and extracted at room temperature for 1 hour, filtered, and the filtrate and residue were collected; the filtrate was concentrated and freeze-dried to obtain Panax notoginseng root extract A;

[0060] (3) The filter residue from step (2) was mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating at 50°C under reduced pressure for 4 h; then ultrasonically treated for 30 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0061] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 4:1; the mass ratio of the surfactant to the organic solvent is 5:100;

[0062] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 30% and the volume concentration of ethyl acetate is 50%;

[0063] (4) Mixing the Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) to obtain a Panax notoginseng root extract.

[0064] Example 4

[0065] A preparation method of Panax notoginseng root extract is as follows:

[0066] (1) Cutting Panax notoginseng root segments (1-2 cm) into sections, mixing with a 0.1% formic acid aqueous solution, soaking for 24 hours, and then steam-exploding the sections at 1.0 MPa for 2 minutes to obtain steam-exploded products;

[0067] (2) The steam explosion-treated product was mixed with a 0.1% by volume formic acid aqueous solution at a solid-liquid ratio of 1:20 g / mL, stirred and extracted at room temperature for 2 h, filtered, and the filtrate and residue were collected; the filtrate was concentrated and freeze-dried to obtain Panax notoginseng root extract A;

[0068] (3) The filter residue from step (2) was mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:35 g / mL, and extracted by heating under reduced pressure at 55°C for 3 h; then ultrasonically treated for 25 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0069] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 5:100;

[0070] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 20% and the volume concentration of ethyl acetate is 55%;

[0071] (4) Mixing the Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) to obtain a Panax notoginseng root extract.

[0072] Example 5

[0073] The difference from Example 1 is that step (3) of the preparation method is different:

[0074] Step (1) to step (2) are the same as in Example 1;

[0075] (3) The filter residue from step (2) was mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating under reduced pressure at 40°C for 6 h; then subjected to ultrasonic treatment for 60 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0076] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0077] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0078] Step (4) is the same as in Example 1.

[0079] Comparative Example 1

[0080] Different from Example 1, the steam explosion treatment in step (1) and the leaching treatment in step (2) were not performed.

[0081] A preparation method of Panax notoginseng root extract is as follows:

[0082] Panax notoginseng root segments (1-2 cm) were mixed with a surfactant and an organic solvent at a solid-to-liquid ratio of 1:30 g / mL and extracted by heating at 50°C under reduced pressure for 4 hours. The extraction was then ultrasonicated for 40 minutes and filtered. The filtrate was concentrated under reduced pressure, the organic solvent was removed, and the extract was freeze-dried to obtain the Panax notoginseng root extract.

[0083] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0084] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%.

[0085] Comparative Example 2

[0086] The difference from Example 1 is that the surfactant in step (3) is different, and polyethylene glycol 200 is not used in this comparative example.

[0087] Step (1) to step (2) are the same as in Example 1;

[0088] (3) The filter residue from step (2) was mixed with Tween 80 and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating at 50°C under reduced pressure for 4 h; then subjected to ultrasonic treatment for 40 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0089] The mass ratio of Tween 80 to organic solvent is 0.875:100;

[0090] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0091] Step (4) is the same as in Example 1.

[0092] Comparative Example 3

[0093] The difference from Example 1 is that the surfactant in step (3) is different, and Tween 80 is not used in this comparative example.

[0094] Step (1) to step (2) are the same as in Example 1;

[0095] (3) The filter residue from step (2) was mixed with polyethylene glycol 200 and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating at 50°C under reduced pressure for 4 h; then subjected to ultrasonic treatment for 40 min and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract B;

[0096] The mass ratio of polyethylene glycol 200 to organic solvent is 2.625:100;

[0097] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0098] Step (4) is the same as in Example 1.

[0099] Comparative Example 4

[0100] The difference from Example 1 is that no surfactant is used in step (3). The rest is the same as Example 1.

[0101] Comparative Example 5

[0102] The difference from Example 1 is that the volume concentration of the organic solvent in step (3) is different.

[0103] In this comparative example, the organic solvent in step (3) is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 55% and the volume concentration of ethyl acetate is 25%.

[0104] Comparative Example 6

[0105] The difference from Example 1 is that the organic solvent extraction treatment is performed first, and then the steam explosion treatment is performed.

[0106] A preparation method of Panax notoginseng root extract is as follows:

[0107] (1) Cut the Panax notoginseng root segments (1-2 cm) into sections, mix them with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extract them by heating at 50°C under reduced pressure for 4 h; then ultrasonicate them for 40 min and filter them to obtain a filtrate and a filter residue; the filtrate is concentrated under reduced pressure, the organic solvent is removed, and then freeze-dried to obtain Panax notoginseng root extract A;

[0108] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0109] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0110] (2) mixing the filter residue with a 0.1% by volume formic acid aqueous solution, soaking for 12 hours, and then performing a steam explosion treatment at 1.0 MPa for 2 minutes to obtain a steam-exploded product;

[0111] (3) The steam explosion-treated product was mixed with a 0.1% by volume formic acid aqueous solution at a solid-liquid ratio of 1:25 g / mL, stirred and extracted at room temperature for 1 h, filtered, and the filtrate was collected; the filtrate was concentrated and freeze-dried to obtain Panax notoginseng root extract B;

[0112] (4) Mixing the Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) to obtain a Panax notoginseng root extract.

[0113] Experiment 1 Antioxidant activity detection

[0114] 1. Preparation of DPPH alcohol solution: Accurately weigh 20 mg of DPPH (1,1-diphenyl-2-trinitrophenylhydrazine), dissolve it in anhydrous ethanol and dilute to a 500 mL brown volumetric flask to obtain a 0.004% DPPH solution. Protect from light and set aside.

[0115] 2. Preparation of sample solution: Weigh the Panax notoginseng root extract of each example and comparative example, dissolve it in water, make the volume to 10 mL, dilute it, and prepare 1 mg / mL to prepare a sample solution.

[0116] 3. Add 1 mL of sample solution to 1 mL of DPPH alcohol solution; mix thoroughly with a homogenizer and protect from light. Measure the absorbance at 517 nm. Repeat the above experiment 3 times and calculate the average value.

[0117] The calculation formula for the scavenging rate of Panax notoginseng root extract on DPPH free radicals is:

[0118] DPPH free radical scavenging rate (%) = 1-[(A i -A j ) / A0]×100, where A0 is the absorbance of 1 mL DPPH alcohol solution + 1 mL water, and A i A is the absorbance value of 1mL DPPH alcohol solution + 1mL sample solution, j It is the absorbance value of 1 mL sample solution + 1 mL water.

[0119] Table 1 shows the DPPH free radical scavenging rate results of the Panax notoginseng root extracts prepared in various examples and comparative examples.

[0120] Table 1

[0121]

[0122]

[0123] In Table 1, the comparative example is compared with Example 1. # P≤0.05, ## P≤0.01.

[0124] As can be seen from Table 1, the Panax notoginseng root extract obtained by the preparation method of the present invention has significant DPPH free radical scavenging activity. The DPPH free radical scavenging rate of the Panax notoginseng root extract obtained by the method of the embodiment is significantly higher than that of the comparative example. Therefore, the preparation method of the present invention can significantly improve the antioxidant activity.

[0125] Experiment 2: Detection of tumor cell proliferation inhibition

[0126] 1. Tumor Cell Culture: Human lung cancer (A549) and hepatoma (HepG2) cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 10 μg / mL penicillin-streptomycin at 37°C, 5% carbon dioxide, and saturated humidity. Cells were digested with trypsin-EDTA solution (0.25%:0.02%) and passaged every two days.

[0127] 2. Experimental samples: Panax notoginseng root extracts prepared in Examples 1 to 5 and Comparative Examples 1 to 6.

[0128] The MTT assay was used to detect the inhibitory effect of Panax notoginseng root extract (0.4 mg / mL) on tumor cell proliferation. The inhibition rates on human lung cancer cells and human liver cancer cells were shown in Table 2.

[0129] Table 2

[0130]

[0131]

[0132] In Table 2, in the same column of data, the comparative example is compared with Example 1. # P≤0.05, ## P≤0.01.

[0133] As can be seen from Table 2, Example 1 exhibited a significantly higher inhibitory effect on tumor cell proliferation than the control group. Furthermore, each Example group exhibited significantly improved inhibitory effects on tumor cell proliferation compared to the control group. The Panax notoginseng root extract obtained using the preparation method of the present invention exhibited significantly enhanced anti-tumor efficacy.

[0134] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a Panax notoginseng root extract, characterized in that: Including steps: (1) After the Panax notoginseng fibrous roots are soaked in a weakly acidic aqueous solution, they are subjected to steam explosion treatment at a pressure of 0.5-1.2 MPa for 1-3 minutes to obtain a steam-exploded product; (2) mixing the steam explosion-treated product with a weakly acidic aqueous solution, extracting, filtering, and collecting the filtrate and filter residue; drying the filtrate to obtain Panax notoginseng root extract A; (3) Mixing the filter residue from step (2) with a surfactant and an organic solvent, heating and extracting at 40-55°C for 3-6 hours; ultrasonically treating for 25-60 minutes, filtering, and drying the filtrate to obtain Panax notoginseng root extract B; The surfactant is polyethylene glycol 200 and Tween 80 in a weight ratio of 2-4:1; The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 15-30% and the volume concentration of ethyl acetate is 50-60%; (4) The Panax notoginseng root extract A of step (2) and the Panax notoginseng root extract B of step (3) are mixed to obtain a Panax notoginseng root extract.

2. The preparation method according to claim 1, characterized in that In step (1) and step (2), the weakly acidic aqueous solution is a formic acid aqueous solution with a volume concentration of 0.05-0.2%.

3. The preparation method according to claim 1, characterized in that In step (1), the soaking time is 8-24 hours.

4. The preparation method according to claim 1, characterized in that In step (1), the steam explosion treatment condition is 1.0 MPa for 2 min.

5. The preparation method according to claim 1, characterized in that In step (2), the extraction is specifically as follows: stirring and extracting at room temperature for 0.5-2h at a solid-to-liquid ratio of 1:20-30g / mL.

6. The preparation method according to claim 1, characterized in that In step (3), the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; The organic solvent is a mixture of ethanol, ethyl acetate and water; wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%.

7. The preparation method according to claim 1, characterized in that In step (3), the mass ratio of the surfactant to the organic solvent is 2.5-5:

100.

8. The preparation method according to claim 1, characterized in that Step (3) is specifically as follows: mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:25-35 g / mL, and extracting by heating under reduced pressure at 45-50°C for 4-5 hours; ultrasonically treating for 30-50 minutes, filtering, concentrating the filtrate under reduced pressure, removing the organic solvent, and drying to obtain Panax notoginseng root extract B.

9. A Panax notoginseng root extract, characterized in that The invention is prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the Panax notoginseng root extract according to claim 9 in the preparation of a medicament having the effect of inhibiting the proliferation of human lung cancer and liver cancer cells.

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