Application of betula platyphylla BpSPL2 gene in improvement of drought tolerance of betula platyphylla

CN119082138APending Publication Date: 2024-12-06NORTHEAST FORESTRY UNIV
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Patent Information

Application Number
CN202411489782.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-24
Publication Date
2024-12-06

AI Technical Summary

Technical Problem

In the prior art, there are few studies on the BpSPL2 gene in improving the drought tolerance of white birch, and there is a lack of relevant functions, making it difficult to effectively improve the drought stress response ability of perennial woody plants.

Method used

By constructing an overexpression vector or an inhibitory expression vector of the BpSPL2 gene, the Agrobacterium infection method was used to genetically transform white birch, and transgenic lines that overexpress or inhibit the BpSPL2 gene were screened to improve or reduce white birch's drought tolerance. Specific methods include overexpression using pGWB5-BpSPL2-GFP vector or inhibition of expression using pROKII-BpSPL2-SRDX vector.

Benefits of technology

Overexpression of the BpSPL2 gene of white birch has been achieved to improve the drought tolerance of plants, provide a regulatory mechanism for participating in the drought resistance process of white birch, and provides a reference resource for the research on the function of plant SBP gene family, which can significantly improve or reduce the drought tolerance of plants.

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Abstract

The invention provides application of a white birch BpSPL2 gene in improving the drought tolerance of white birches, and belongs to the technical field of molecular biology. The invention provides over-expression of the BpSPL2 gene participating in drought tolerance of the white birch, inhibits expression of transgenic white birch plants, and can also be used for excavating downstream regulation genes and enriching regulation pathways for plant growth stress resistance; the 35S:: BpSPL2 white birch plant obtained by overexpressing the BpSPL2 gene can be used for cultivating forest trees with excellent stress-resistant growth traits, wherein the drought tolerance of the 35S:: BpSPL2 is improved; the BpSPL2 gene provided by the invention can participate in the drought resisting process of white birch, can be used for stress growth and development research of forest plants, and provides reference resources for SBP gene family function research of the plants.
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Citation Information

Patent Citations

  • White birch SPL2 gene participating plant morphogenesis and floral development and protein thereof

    CN107475263A

  • Key gene TcSBP5 regulating salt tolerance of tamarix chinensis and application of key gene TcSBP5

    CN109988775A

  • Application of white birch BpSPL2 gene in regulation and control of adventitious root development of white birch

    CN111690664A

  • Application of betula platyphylla BpPIF4 gene in improvement of drought resistance of betula platyphylla

    CN117625673A

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