A triglyceride determination kit and a triglyceride determination method

Abstract

The present invention relates to the technical field of triglyceride determination, and in particular to a triglyceride determination kit and a determination method thereof. In the present invention, a biochemical reaction and optical measurement module obtains a serum sample, uses a triglyceride reaction method and a sarcosine reaction method to generate triglyceride colored compounds and sarcosine colored compounds, and uses a specific wavelength light source to measure their absorbance, a data conversion module digitizes their absorbance and records a timestamp, and a data analysis module establishes a mathematical model of sarcosine change rate and triglyceride concentration, and uses the established mathematical model to adjust the triglyceride concentration through the sarcosine concentration; this method solves the limitation of traditional single indicator determination, and improves the accuracy and reliability of triglyceride concentration measurement by introducing sarcosine as an auxiliary indicator. This technology can not only capture dynamic metabolic changes, but also effectively reduce biological interference, and provide more comprehensive and accurate data support for clinical diagnosis and research.

Description

Technical Field

[0001] The present invention relates to the technical field of triglyceride determination, in particular to a triglyceride determination kit and a determination method thereof. Background Art

[0002] There are several key technical problems in traditional triglyceride determination kits and methods.

[0003] First, they usually only focus on the static concentration measurement of triglycerides and ignore the dynamic changes in the metabolic state of the human body, which may lead to the measurement results that cannot accurately reflect the actual physiological condition. Secondly, traditional methods often do not consider the impact of other related metabolic indicators on triglyceride measurement, which may introduce systematic errors. Furthermore, due to the lack of an effective correction mechanism, traditional methods are difficult to eliminate or reduce biological interference in the detection process, affecting the accuracy and reliability of the measurement. In addition, traditional test kits usually only measure a single indicator and cannot provide more comprehensive metabolic status information, limiting its application value in complex metabolic disorders. At the same time, traditional methods also have limitations in continuous monitoring and capturing small concentration changes, which may lead to missing important physiological change information.

[0004] Finally, due to the lack of effective data integration and analysis mechanisms, traditional methods are unable to fully utilize measurement data to provide deeper clinical diagnosis and research support, thus limiting their application potential in medical decision-making and scientific research. Summary of the invention

[0005] The purpose of the present invention is to provide a triglyceride determination kit and a determination method thereof to solve the problems raised in the above background technology.

[0006] To achieve the above object, one of the objects of the present invention is to provide a triglyceride determination kit, comprising a biochemical reaction and optical measurement module, a data conversion module and a data analysis module, wherein:

[0007] The biochemical reaction and optical measurement module obtains a serum sample, and uses a triglyceride reaction method and a sarcosine reaction method to generate a triglyceride colored compound and a sarcosine colored compound for the serum sample respectively; optically measures the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction based on the triglyceride colored compound and the sarcosine colored compound;

[0008] The data conversion module converts the absorbance electrical signals of the triglyceride reaction and the sarcosine reaction into digital signals by using an analog-to-digital converter, and records the corresponding timestamps for digital analysis and processing; and calculates the triglyceride concentration and the sarcosine concentration respectively according to the converted digital signals;

[0009] The data analysis module calculates the rate of change of sarcosine concentration according to the rate of change of sarcosine concentration and the corresponding timestamp, establishes a mathematical model to describe their correlation according to the relationship between the rate of change of sarcosine concentration and triglyceride concentration, and uses the established mathematical model to adjust the triglyceride concentration through the sarcosine concentration.

[0010] As a further improvement of the technical solution, the biochemical reaction and optical measurement module includes a biochemical reaction unit, and the process of generating triglyceride colored compounds and sarcosine colored compounds by the biochemical reaction unit specifically includes:

[0011] Blood samples were collected from patients and research subjects, and serum samples were separated by centrifugation;

[0012] preparing a triglyceride reagent, the triglyceride reagent comprising lipase, glycerol kinase, glycerol phosphate oxidase and peroxidase, mixing a serum sample with the triglyceride reagent, hydrolyzing triglyceride into glycerol and fatty acid by lipase, phosphorylating glycerol into glycerol-3-phosphate by glycerol kinase, oxidizing glycerol-3-phosphate by glycerol phosphate oxidase to generate triglyceride hydrogen peroxide, catalyzing triglyceride hydrogen peroxide by peroxidase, and reacting the triglyceride hydrogen peroxide with a color developer to generate a triglyceride colored compound;

[0013] A sarcosine reagent is prepared, the sarcosine reagent includes sarcosine oxidase and peroxidase, the serum sample is mixed with the sarcosine reagent, sarcosine is oxidized by the sarcosine oxidase to generate sarcosine hydrogen peroxide, the sarcosine hydrogen peroxide is catalyzed by the peroxidase, and reacted with a color developer to generate a sarcosine colored compound.

[0014] As a further improvement of the technical solution, the biochemical reaction and optical measurement module includes an optical measurement unit, and the process of the optical measurement unit generating the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction specifically includes:

[0015] preparing light sources of specific wavelengths for triglyceride colored compounds and sarcosine colored compounds respectively;

[0016] The triglyceride colored compound and the sarcosine colored compound are transferred to special cuvettes respectively. During measurement, a photodetector is used to record a light beam emitted from a light source as the incident light intensity. The light beam passes through a cuvette containing a reaction mixture, and part of the light is absorbed by the colored compound. The light passing through the cuvette is recorded again by the photodetector as the transmitted light intensity. The absorbance value is calculated by comparing the incident light intensity and the transmitted light intensity, and finally the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction are obtained, wherein the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction are electrical signals.

[0017] As a further improvement of the present technical solution, the specific wavelength range is within 500-550 nm.

[0018] As a further improvement of the technical solution, the process of calculating the triglyceride concentration and the creatine concentration by the data conversion module specifically includes:

[0019] The absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction are converted into the absorbance digital signals of the triglyceride reaction and the sarcosine reaction by using an analog-to-digital converter. During the conversion process, the analog-to-digital converter samples, quantizes and encodes the electrical signals.

[0020] Record the corresponding timestamp for each converted digital signal. The timestamp is used to track the exact time of measurement, which is helpful for subsequent data analysis and quality control.

[0021] The triglyceride concentration and the sarcosine concentration are calculated respectively according to the absorbance digital signal of the triglyceride reaction and the absorbance digital signal of the sarcosine reaction by applying the Beer-Lambert law.

[0022] As a further improvement of the technical solution, the process of adjusting the triglyceride concentration by the data analysis module specifically includes:

[0023] Utilize the sarcosine concentration and corresponding timestamp provided by the data conversion module;

[0024] For every two consecutive time points (t1, t2), the change rate of sarcosine concentration Z is calculated, Z = (C2-C1) / (t2-t1), where C1 and C2 are the sarcosine concentrations at t1 and t2, respectively;

[0025] A mathematical model was established to describe the relationship between the change rate of sarcosine concentration and triglyceride concentration. The triglyceride concentration data at the corresponding time points were collected and a mathematical model was established using the regression analysis method. The form of the mathematical model is as follows:

[0026] TG_adjusted=TG_measured+f(dCr / dt), where TG_adjusted is the adjusted triglyceride concentration, TG_measured is the triglyceride concentration, and f(dCr / dt) is a function of the rate of change of creatine concentration;

[0027] For each measurement point, the adjustment value is calculated using the established mathematical model and the corresponding sarcosine concentration change rate as the adjusted triglyceride concentration;

[0028] Generates a result report containing the raw triglyceride concentration, adjusted triglyceride concentration, creatinine concentration, and the rate of change of creatinine concentration with corresponding timestamps.

[0029] The second object of the present invention is to provide a method for a triglyceride determination kit, comprising the following steps:

[0030] S1. Obtaining a serum sample, and using a triglyceride reaction method and a sarcosine reaction method to generate a triglyceride colored compound and a sarcosine colored compound respectively; and optically measuring the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction based on the triglyceride colored compound and the sarcosine colored compound;

[0031] S2, using an analog-to-digital converter to convert the absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction into digital signals, and record the corresponding timestamps for digital analysis and processing; and according to the converted digital signals, respectively calculate the triglyceride concentration and the sarcosine concentration;

[0032] S3. Calculate the rate of change of sarcosine concentration according to the rate of change of sarcosine concentration and the corresponding timestamp. Establish a mathematical model to describe their correlation according to the relationship between the rate of change of sarcosine concentration and triglyceride concentration. Use the established mathematical model to adjust the triglyceride concentration according to the sarcosine concentration.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] 1. The triglyceride determination kit and its determination method select sarcosine as an auxiliary indicator to adjust the triglyceride concentration measurement result, which has many advantages; first, sarcosine and triglycerides are closely related in the metabolic process, and they are both important indicators of energy metabolism, especially in certain metabolic disorders (such as obesity, diabetes, etc.), these two indicators often change at the same time, providing an opportunity for mutual verification and correction; secondly, the determination method of sarcosine is similar to that of triglycerides, both using enzymatic methods, which means that the two tests can be integrated in the same kit, using similar instruments and equipment, simplifying the entire measurement process and improving efficiency; in addition, as a product of muscle metabolism, the concentration change of sarcosine can reflect the dynamic changes of the body state, and this dynamic information can be used to compensate for possible systematic errors or biological interferences in triglyceride measurements, thereby improving the accuracy and reliability of the measurement results.

[0035] 2. This triglyceride determination kit and its determination method calculate the rate of change of sarcosine concentration and establish a mathematical model of its relationship with triglyceride concentration. This method realizes multiple applications of sarcosine data and produces significant beneficial effects. First, the calculation of the rate of change of sarcosine concentration takes into account the time factor and can capture the dynamic changes of physiological state, which provides more valuable information than simple static concentration measurement; secondly, the established mathematical model not only describes the correlation between sarcosine and triglycerides, but also quantifies this relationship, making the correction process more accurate and reliable. Finally, using this model to adjust the triglyceride concentration can effectively eliminate or reduce systematic errors and biological interferences in the detection process; this method not only improves the accuracy of single measurements, but also captures more subtle concentration changes in continuous monitoring, providing more reliable data support for clinical diagnosis and research, and ultimately enhancing the credibility of relevant medical decisions and scientific research conclusions. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the overall module unit of the present invention;

[0037] Figure 2 It is a schematic diagram of the overall method flow of the present invention.

[0038] In the figure: 100, biochemical reaction and optical measurement module; 101, biochemical reaction unit; 102, optical measurement unit; 200, data conversion module; 300, data analysis module. DETAILED DESCRIPTION

[0039] The following will be combined with the accompanying drawings in the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Unless otherwise specified, the terms (including technical terms) used herein have the commonly understood meanings to those skilled in the art. In addition, it is understood that the terms defined in commonly used dictionaries should be understood to have the same meanings as those in the context of the relevant fields, and should not be understood as idealized or overly formal meanings.

[0041] According to the document, the following are some of the definitions of terms:

[0042] Triglyceride colored compound: a compound with a specific color produced by the reaction of triglyceride reagent with serum sample, the color depth of which is related to the triglyceride concentration;

[0043] Sarcosine colored compound: a compound with a specific color produced by the reaction of sarcosine reagent with serum sample, the color depth of which is related to the sarcosine concentration;

[0044] Absorbance of triglyceride reaction: the degree to which triglyceride colored compounds absorb light under illumination of a specific wavelength (in the range of 500-550nm), expressed in the form of an electrical signal;

[0045] Absorbance of sarcosine reaction: the degree to which sarcosine colored compounds absorb light under illumination of a specific wavelength (within the range of 500-550nm), expressed in the form of an electrical signal;

[0046] Triglyceride concentration: The triglyceride content in serum calculated from the absorbance digital signal of triglyceride reaction by Beer-Lambert law;

[0047] Creatine concentration: the content of creatine in serum calculated from the absorbance digital signal of the creatine reaction using the Beer-Lambert law;

[0048] Mathematical model: A mathematical expression describing the relationship between the rate of change of sarcosine concentration and triglyceride concentration in the form of TG_adjusted=TG_measured+f(dCr / dt), which is used to adjust the measured triglyceride concentration by the rate of change of sarcosine concentration to improve the accuracy of the result.

[0049] Next, see Figure 1-Figure 2 One of the purposes of this embodiment is to provide a triglyceride determination kit, which includes a biochemical reaction and optical measurement module 100, a data conversion module 200 and a data analysis module 300.

[0050] The biochemical reaction unit 101 in the biochemical reaction and optical measurement module 100 obtains a serum sample, and uses a triglyceride reaction method and a sarcosine reaction method to generate a triglyceride colored compound and a sarcosine colored compound on the serum sample, specifically including:

[0051] Collect blood samples from patients or research subjects and separate serum samples by centrifugation or other methods;

[0052] preparing a triglyceride reagent, the triglyceride reagent comprising lipase, glycerol kinase, glycerol phosphate oxidase and peroxidase, mixing a serum sample with the triglyceride reagent, hydrolyzing triglyceride into glycerol and fatty acid by lipase, phosphorylating glycerol into glycerol-3-phosphate by glycerol kinase, oxidizing glycerol-3-phosphate by glycerol phosphate oxidase to generate triglyceride hydrogen peroxide, catalyzing triglyceride hydrogen peroxide by peroxidase, and reacting the triglyceride hydrogen peroxide with a color developer to generate a triglyceride colored compound;

[0053] A sarcosine reagent is prepared, the sarcosine reagent includes sarcosine oxidase and peroxidase, the serum sample is mixed with the sarcosine reagent, sarcosine is oxidized by the sarcosine oxidase to generate sarcosine hydrogen peroxide, the sarcosine hydrogen peroxide is catalyzed by the peroxidase, and reacted with a color developer to generate a sarcosine colored compound.

[0054] Since both sarcosine and triglycerides are closely related to energy metabolism, sarcosine is an important product of muscle energy metabolism, while triglycerides are the main form of fat metabolism. In certain metabolic disorders (such as obesity, diabetes, etc.), these two indicators may change at the same time, providing an opportunity for mutual verification. In addition, sarcosine is determined by enzymatic method, which is similar to the determination method of triglycerides, which means that the two tests can be integrated in the same kit without the need to introduce completely different measurement technologies. The two tests can use similar instruments and equipment, simplifying the entire measurement process.

[0055] The optical measurement unit 102 in the biochemical reaction and optical measurement module 100 generates the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction by optical measurement according to the triglyceride colored compound and the sarcosine colored compound, specifically including:

[0056] A light source with a specific wavelength is prepared for the triglyceride colored compound and the sarcosine colored compound, wherein the wavelength range is within 500-550 nm, which is located in the green to yellow-green region of the visible spectrum. This range is selected because most of the triglyceride colored compounds and the sarcosine colored compounds have maximum absorption within this range. Within this wavelength range, the colored compounds produced by the reaction have the strongest absorption of light, providing the best sensitivity and measurement accuracy;

[0057] The triglyceride colored compound and the sarcosine colored compound are transferred to special cuvettes respectively. During measurement, a photodetector is used to record a light beam emitted from a light source as the incident light intensity. The light beam passes through a cuvette containing a reaction mixture, and part of the light is absorbed by the colored compound. The light passing through the cuvette is recorded again by the photodetector as the transmitted light intensity. The absorbance value is calculated by comparing the incident light intensity and the transmitted light intensity, and finally the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction are obtained, wherein the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction are electrical signals.

[0058] The calculation process in the optical measurement unit 102 can not only measure the concentrations of triglycerides and creatine separately, but also measure the two substances at the same time, thereby improving the measurement efficiency; the absorbance data in the form of electrical signals finally obtained lays the foundation for subsequent digital processing and analysis, so that the entire measurement process not only ensures the accuracy and reliability of the data, but also improves the overall measurement efficiency.

[0059] The data conversion module 200 uses an analog-to-digital converter to convert the absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction into digital signals, and records the corresponding timestamps for digital analysis and processing; according to the converted digital signals, the triglyceride concentration and the sarcosine concentration are calculated respectively, specifically including:

[0060] The absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction are converted into the absorbance digital signals of the triglyceride reaction and the sarcosine reaction by using an analog-to-digital converter. During the conversion process, the analog-to-digital converter samples, quantizes and encodes the electrical signals.

[0061] Record the corresponding timestamp for each converted digital signal. The timestamp is used to track the exact time of measurement, which is helpful for subsequent data analysis and quality control.

[0062] The triglyceride concentration and the sarcosine concentration are calculated respectively according to the absorbance digital signal of the triglyceride reaction and the absorbance digital signal of the sarcosine reaction by applying the Beer-Lambert law.

[0063] The data analysis module 300 calculates the change rate of the sarcosine concentration according to the change rate of the sarcosine concentration and the corresponding timestamp, establishes a mathematical model to describe the correlation between the change rate of the sarcosine concentration and the triglyceride concentration according to the relationship between the change rate of the sarcosine concentration and the triglyceride concentration, and uses the established mathematical model to adjust the triglyceride concentration according to the sarcosine concentration, specifically including:

[0064] Using the creatine concentration and the corresponding timestamp provided by the data conversion module 200,

[0065] For every two consecutive time points (t1, t2), the change rate of sarcosine concentration Z is calculated, Z = (C2-C1) / (t2-t1), where C1 and C2 are the sarcosine concentrations at t1 and t2, respectively;

[0066] A mathematical model was established to describe the relationship between the change rate of sarcosine concentration and triglyceride concentration. The triglyceride concentration data at the corresponding time points were collected and a mathematical model was established using the regression analysis method. The form of the mathematical model is as follows:

[0067] TG_adjusted=TG_measured+f(dCr / dt), where TG_adjusted is the adjusted triglyceride concentration, TG_measured is the triglyceride concentration, and f(dCr / dt) is a function of the rate of change of creatine concentration;

[0068] For each measurement point, the adjustment value is calculated using the established mathematical model and the corresponding sarcosine concentration change rate as the adjusted triglyceride concentration;

[0069] Generates a result report containing the raw triglyceride concentration, adjusted triglyceride concentration, creatinine concentration, and the rate of change of creatinine concentration with corresponding timestamps.

[0070] The triglyceride concentration is adjusted by using the change rate of creatine concentration, in order to eliminate or reduce the systematic error or biological interference in the detection process and improve the accuracy of the triglyceride concentration results.

[0071] The second purpose of this embodiment is to provide a method for a triglyceride determination kit, comprising the following steps:

[0072] S1. Obtaining a serum sample, and using a triglyceride reaction method and a sarcosine reaction method to generate a triglyceride colored compound and a sarcosine colored compound respectively; and optically measuring the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction based on the triglyceride colored compound and the sarcosine colored compound;

[0073] S2, using an analog-to-digital converter to convert the absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction into digital signals, and record the corresponding timestamps for digital analysis and processing; and according to the converted digital signals, respectively calculate the triglyceride concentration and the sarcosine concentration;

[0074] S3. Calculate the rate of change of sarcosine concentration according to the rate of change of sarcosine concentration and the corresponding timestamp. Establish a mathematical model to describe their correlation according to the relationship between the rate of change of sarcosine concentration and triglyceride concentration. Use the established mathematical model to adjust the triglyceride concentration according to the sarcosine concentration.

[0075] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A triglyceride determination kit, characterized in that: It comprises a biochemical reaction and optical measurement module (100), a data conversion module (200) and a data analysis module (300), wherein: The biochemical reaction and optical measurement module (100) obtains a serum sample, and uses a triglyceride reaction method and a sarcosine reaction method to generate a triglyceride colored compound and a sarcosine colored compound respectively; and optically measures the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction based on the triglyceride colored compound and the sarcosine colored compound; The data conversion module (200) converts the absorbance electrical signals of the triglyceride reaction and the creatine reaction into digital signals by using an analog-to-digital converter, and records the corresponding timestamps for digital analysis and processing; and calculates the triglyceride concentration and the creatine concentration respectively according to the converted digital signals; The data analysis module (300) calculates the change rate of the sarcosine concentration according to the change rate of the sarcosine concentration and the corresponding timestamp, establishes a mathematical model to describe the correlation between the change rate of the sarcosine concentration and the triglyceride concentration according to the relationship between the change rate of the sarcosine concentration and the triglyceride concentration, and uses the established mathematical model to adjust the triglyceride concentration according to the sarcosine concentration; The process of adjusting the triglyceride concentration by the data analysis module (300) specifically includes: Utilizing the sarcosine concentration and the corresponding timestamp provided by the data conversion module (200); For every two consecutive time points (t1, t2), the change rate of sarcosine concentration Z is calculated, Z = (C2-C1) / (t2-t1), where C1 and C2 are the sarcosine concentrations at t1 and t2, respectively; A mathematical model was established to describe the relationship between the change rate of sarcosine concentration and triglyceride concentration. The triglyceride concentration data at the corresponding time points were collected and a mathematical model was established using the regression analysis method. The form of the mathematical model is as follows: TG_adjusted=TG_measured+f(dCr / dt), where TG_adjusted is the adjusted triglyceride concentration, TG_measured is the triglyceride concentration, and f(dCr / dt) is a function of the rate of change of creatine concentration; For each measurement point, the adjustment value is calculated using the established mathematical model and the corresponding sarcosine concentration change rate as the adjusted triglyceride concentration; Generates a result report containing the raw triglyceride concentration, adjusted triglyceride concentration, creatinine concentration, and the rate of change of creatinine concentration with corresponding timestamps.

2. The triglyceride determination kit according to claim 1, characterized in that The biochemical reaction and optical measurement module (100) comprises a biochemical reaction unit (101), and the process of generating triglyceride colored compounds and sarcosine colored compounds by the biochemical reaction unit (101) specifically comprises: Blood samples were collected from patients and research subjects, and serum samples were separated by centrifugation; preparing a triglyceride reagent, the triglyceride reagent comprising lipase, glycerol kinase, glycerol phosphate oxidase and peroxidase, mixing a serum sample with the triglyceride reagent, hydrolyzing triglyceride into glycerol and fatty acid by lipase, phosphorylating glycerol into glycerol-3-phosphate by glycerol kinase, oxidizing glycerol-3-phosphate by glycerol phosphate oxidase to generate triglyceride hydrogen peroxide, catalyzing triglyceride hydrogen peroxide by peroxidase, and reacting the triglyceride hydrogen peroxide with a color developer to generate a triglyceride colored compound; A sarcosine reagent is prepared, the sarcosine reagent includes sarcosine oxidase and peroxidase, the serum sample is mixed with the sarcosine reagent, sarcosine is oxidized by the sarcosine oxidase to generate sarcosine hydrogen peroxide, the sarcosine hydrogen peroxide is catalyzed by the peroxidase, and reacted with a color developer to generate a sarcosine colored compound.

3. The triglyceride determination kit according to claim 2, characterized in that: The biochemical reaction and optical measurement module (100) comprises an optical measurement unit (102), and the process of the optical measurement unit (102) generating the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction specifically comprises: preparing light sources of specific wavelengths for triglyceride colored compounds and sarcosine colored compounds respectively; The triglyceride colored compound and the sarcosine colored compound are transferred to special cuvettes respectively. During measurement, a photodetector is used to record a light beam emitted from a light source as the incident light intensity. The light beam passes through a cuvette containing a reaction mixture, and part of the light is absorbed by the colored compound. The light passing through the cuvette is recorded again by the photodetector as the transmitted light intensity. The absorbance value is calculated by comparing the incident light intensity and the transmitted light intensity, and finally the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction are obtained, wherein the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction are electrical signals.

4. The triglyceride determination kit according to claim 3, characterized in that: The specific wavelength range is within 500-550 nm.

5. The triglyceride determination kit according to claim 1, characterized in that: The process of the data conversion module (200) calculating the triglyceride concentration and the creatine concentration specifically includes: The absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction are converted into the absorbance digital signals of the triglyceride reaction and the sarcosine reaction by using an analog-to-digital converter. During the conversion process, the analog-to-digital converter samples, quantizes and encodes the electrical signals. Record the corresponding timestamp for each converted digital signal. The timestamp is used to track the exact time of measurement, which is helpful for subsequent data analysis and quality control. The triglyceride concentration and the sarcosine concentration are calculated respectively according to the absorbance digital signal of the triglyceride reaction and the absorbance digital signal of the sarcosine reaction by applying the Beer-Lambert law.

6. A method for using the triglyceride determination kit according to any one of claims 1 to 5, characterized in that: The method comprises the following steps: S1. Obtaining a serum sample, and using a triglyceride reaction method and a sarcosine reaction method to generate a triglyceride colored compound and a sarcosine colored compound respectively; and optically measuring the absorbance of the triglyceride reaction and the absorbance of the sarcosine reaction based on the triglyceride colored compound and the sarcosine colored compound; S2, using an analog-to-digital converter to convert the absorbance electrical signals of the triglyceride reaction and the absorbance electrical signals of the sarcosine reaction into digital signals, and record the corresponding timestamps for digital analysis and processing; and according to the converted digital signals, respectively calculate the triglyceride concentration and the sarcosine concentration; S3. Calculate the rate of change of sarcosine concentration according to the rate of change of sarcosine concentration and the corresponding timestamp. Establish a mathematical model to describe their correlation according to the relationship between the rate of change of sarcosine concentration and triglyceride concentration. Use the established mathematical model to adjust the triglyceride concentration according to the sarcosine concentration.

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