Seed treatment agent containing difenoconazole, metalaxyl-M and pyraclostrobin and application thereof

Abstract

The invention belongs to the field of pesticides, and particularly relates to a seed treating agent containing difenoconazole, metalaxyl-M and pyraclostrobin, the mass ratio of the difenoconazole to the metalaxyl-M to the pyraclostrobin is (1-5): (2-6): (5-10), the seed treating agent can be used for soybean seed treatment, has a good inhibition effect on various fungi and oomycetes diseases of soybeans, and has a good application prospect. And when the active ingredients have a synergistic effect, the use dosage of the active ingredients can be obviously reduced, the generation of drug resistance of germs can be retarded, and the environmental pollution can be alleviated.

Description

Technical Field

[0001] The invention belongs to the field of pesticides, and specifically relates to a seed treatment agent comprising difenoconazole, metalaxyl-M and pyraclostrobin and application thereof. Background Art

[0002] Soybean is one of the most important crops in the world, playing an important role in agricultural production and social development. With the expansion of soybean planting area, diseases have become one of the main factors affecting its yield. At present, there are more than 40 soybean diseases reported in my country, and the pathogens mainly include fungi, bacteria, nematodes and viruses, of which 70%-80% are fungal diseases, which generally cause a 10%-30% reduction in yield, which can reach more than 50% in severe cases, and even lead to a total loss of production in more severe cases. Soybean fungal diseases not only directly cause a reduction in yield and quality, but some pathogenic fungi also secrete a variety of toxins and metabolites that are harmful to humans and animals during the infection process, posing a great threat to the safety of agricultural products.

[0003] Fenfenac is a triazole fungicide with systemic conduction. When used for seed treatment, it can be quickly absorbed by crop roots and conducted to various parts of the plant. Metalaxyl-M can penetrate the seed coat and be systemically conducted to various parts of the plant as the seeds germinate and grow. When used for seed treatment, it can prevent and control a variety of seed-borne and soil-borne diseases caused by lower fungi. Pyraclostrobin is a methoxyacrylate fungicide with a wide range of fungicides, protection, treatment, good penetration and conduction effects, and plant health effects. When used for seed treatment, it can effectively prevent and control soil-borne and seed-borne diseases on corn.

[0004] There is no related report in the prior art on the combined use of difenoconazole, metalaxyl-M and pyraclostrobin for soybean seed treatment. Summary of the invention

[0005] The object of the present invention is to provide a seed treatment agent comprising difenoconazole, metalaxyl-M and pyraclostrobin and its application. The specific technical scheme is as follows:

[0006] A seed treatment agent comprises difenoconazole, metalaxyl-M and pyraclostrobin, wherein the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 1-5:2-6:5-10.

[0007] Preferably, the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 1.5:5:7.5 or 1:1:1 or 1.7:3.3:5.

[0008] The weight percentage of active ingredients in the seed treatment agent is 10-20%, including auxiliary ingredients. The preferred weight percentage of active ingredients is 10, 14%. The auxiliary ingredients include one or more of wetting and dispersing agents, thickeners, antifreeze agents, film-forming agents, preservatives, warning colors and water. The seed treatment agent is a suspension seed coating agent or a microcapsule suspension-suspension agent.

[0009] The seed treatment agent is used for soybean seed treatment and can be used for preventing and controlling various fungal diseases of soybean.

[0010] Compared with the prior art, the present invention achieves the following beneficial technical effects:

[0011] When the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 3:10:15 and 1:1:1, it has a synergistic effect on Phytophthora sojae; when the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 3:10:15, it has a synergistic effect on Fusarium graminearum. When the active ingredients show a synergistic effect, the dosage of the active ingredients can be significantly reduced, the development of drug resistance of pathogens can be slowed down, and environmental pollution can be reduced.

[0012] Compared with seeds coated with a single agent, the overall control effect of the present invention is more obvious. Especially in formulation 2, when the wetting and dispersing agent is Morwet D-500, At l ox4913, and the film-forming agent is SYFMA001, it helps to exert the efficacy of the drug.

[0013] The microcapsule suspension-suspension concentrate prepared by the present invention designs one of the low-melting-point components as a thin-shell quick-release microcapsule suspension concentrate, which not only overcomes its instability, but also enables the microcapsule to have the dual characteristics of quick release + slow release, that is, it can have a quick effect on the prevention and treatment of soybean root rot in the seedling stage, and solve the various diseases occurring at the base of the soybean stem in the middle and late stages of growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 Plate test of the effects of seed treatment agents on carbon rot and phomopsis

[0015] Figure 2 Plate test of seed treatment agents on red crown rot

[0016] Figure 3 Plate test of seed treatment agents on rotten silk, white silk, stem canker, soybean phytophthora, and ultimum pythium

[0017] Figure 4 Plate test of seed treatment agents on the effects of Fusarium rot on eggplant

[0018] Figure 5 Plate test of seed treatment agents on Sickle of Cereals

[0019] Figure 6Plate test of pathogenic fungi in soybeans of different treatment groups DETAILED DESCRIPTION

[0020] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0021] The present invention is further explained below in conjunction with specific implementation modes.

[0022] Example 1: Indoor bioassay of compound agents against soybean root rot pathogens

[0023] Test agents: difenoconazole technical, metalaxyl-M technical, pyraclostrobin technical;

[0024] Test pathogens: Phytophthora sojae Kaufmann; Fusarium graminearum.

[0025] Test method: Mycelium growth rate method was used.

[0026] Activate the test pathogen strains on the culture medium. Based on the preliminary experiment, dilute the mother solution of the test agent with sterile water into 7 corresponding concentration gradients. Pipette 2mL of the prepared drug solution into a sterile culture dish, pour 18mL of molten culture medium, mix thoroughly and let stand to cool, and obtain a drug-containing plate with a suitable final concentration. Use a 5mm diameter puncher to make a bacterial plate with the activated test strain colony, and use an inoculation loop to place the bacterial plate in the center of the drug-containing culture medium plate with the mycelium facing down. Repeat each treatment concentration 5 times, and use the drug-free plate as a control. After inoculation, place it in a biological incubator for a period of time, measure the colony diameter (mm) by the cross method, and calculate the mycelium growth inhibition rate.

[0027] Mycelial growth inhibition rate = [(control colony diameter - treated colony diameter) / (control colony diameter - 5)] × 100%;

[0028] The concentration of the drug was converted into a logarithm (x), and the inhibition rate was converted into a probability value (y). Based on the linear relationship between x and y, the toxicity regression curve equation y = a + bx, the correlation coefficient (r), and the effective inhibition concentration (EC 50 ).

[0029] Determination of the combined toxicity and co-toxicity coefficient of compound preparations: On the basis of determining the toxicity of a single preparation, the combined toxicity of each mixture in different combinations and proportions was determined respectively, and then the theoretical toxicity index TTI, actual toxicity index ATI and co-toxicity coefficient CTC of each mixture were calculated.

[0030] When CTC≥120, it is determined that the drugs have a synergistic effect; when 80<CTC<120, the drugs have an additive effect; when CTC≤80, the drugs have an antagonistic effect. The synergistic effect of the compound is evaluated according to the size of the co-toxicity coefficient, and the optimal ratio is determined.

[0031] Test results:

[0032] As shown in Table 1, the EC values ​​of difenoconazole, metalaxyl-M and pyraclostrobin against Phytophthora sojae 50 (μg·mL -1 ) were 3.106, 0.3728 and 0.5425 respectively; when the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin was 3:10:15 and 1:1:1, the EC 50 (μg·mL -1 ) were 0.256 and 0.5024, respectively, and the CTC values ​​were 197.3 and 123.2, respectively, showing a synergistic effect; in particular, when the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin was 3:10:15, a significant synergistic effect was shown. When the composition shows a synergistic effect, the dosage of the active ingredient can be significantly reduced, the generation of bacterial resistance can be slowed down, and environmental pollution can be reduced.

[0033] Table 1: Indoor bioassay of the combination of pesticides against Phytophthora sojae

[0034]

[0035] As shown in Table 2, the EC values ​​of difenoconazole, metalaxyl-M and pyraclostrobin against Fusarium graminearum 50 (μg·mL -1 ) were 1.8591, 1.1204 and 4.9662 respectively; when the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin was 3:10:15, the EC 50 (μg·mL -1 ) is 1.5981, and the CTC value is 129.2, showing a synergistic effect; when the composition shows a synergistic effect, the dosage of the active ingredient can be significantly reduced, the generation of bacterial resistance can be slowed down, and environmental pollution can be reduced.

[0036] Table 2: Indoor bioassay of the combined pesticides against Fusarium graminearum in soybean

[0037]

[0038] Example 2: Indoor bioassay of compound agents against other soybean pathogens

[0039] Based on the combined action data of Example 1, a compound synergistic agent with a mass ratio of 3:10:15 of difenoconazole, metalaxyl-M and pyraclostrobin was selected for indoor bioassay. The test method is referenced to Example 1.

[0040] Table 3 Indoor bioassay of compound pesticides against other soybean pathogens

[0041]

[0042]

[0043] Example 3: Preparation of Suspended Seed Coating Agent

[0044] Preparation 1: 14% suspension seed coating agent, containing the following weight percentage ingredients:

[0045] 7.5% pyraclostrobin, 5% metalaxyl-M, 1.5% difenoconazole, 3.0% Morwet D-425, 1.0% NNO, 1.0% calcium lignin sulfonate, 0.3% magnesium aluminum silicate, 0.1% xanthan gum, 2.5% film-forming agent SYFMA001, 4.0% ethylene glycol, 0.5% sodium benzoate, 0.5% organic bentonite, and deionized water to make up to 100%.

[0046] Preparation method: According to the type and amount of each component in the formula, other components except the film-forming agent, such as active ingredients, wetting and dispersing agents, thickeners, antifreeze agents, preservatives and water are mixed and pre-dispersed, and the sample is pretreated using a high-shear emulsifier. The material is then transferred to a sand mill for sand grinding. After sand grinding for a period of time, the particle size is measured. When the particle size value reaches the required requirement, the sand grinding is terminated, and finally the film-forming agent is added and the mixture is obtained after homogenous stirring.

[0047] Formulation 2: 14% suspension seed coating agent, containing the following weight percentage ingredients:

[0048] 7.5% pyraclostrobin, 5% metalaxyl-M, 1.5% difenoconazole, 3.0% Morwet D-500, 2% At lox4913, 0.3% magnesium aluminum silicate, 0.1% xanthan gum, 2.5% film-forming agent SYFMA001, 4.0% ethylene glycol, 0.5% sodium benzoate, 0.5% organic bentonite, and deionized water to make up to 100%.

[0049] The preparation method is the same as above.

[0050] Example 4: Effect of 14% suspension seed coating on soybean germination

[0051] Test agent:

[0052] Table 4: Test agents

[0053] deal with Pharmacy Dosage (ml / 100 kg bean seeds) Blank control - — 1 14% phenyl ether, methyl pyrazole (preparation 2) 200 2 3% difenoconazole suspension seed coating agent 200 3 20% Metalaxyl-Med Suspension Seed Coating Agent 200 4 25g / L Fludioxonil Suspension Seed Coating Agent 200 5 10% chlorpyrifos·fine frost suspension seed coating agent 200

[0054] Depend on Figure 6 It can be seen that compared with uncoated soybean seeds, the suspension seed coating agent composed of 7.5% pyraclostrobin, 5% metalaxyl and 1.5% fenpropimorph significantly improved the germination rate and sprout length of soybeans, and significantly reduced the incidence rate and average lesion length, and had a significant inhibitory effect on soybean pathogenic fungi.

[0055] Example 5: Field efficacy of 14% suspension seed coating agent on soybeans

[0056] Test method: Random block sorting, test repeated 4 times, plot area 30m 2 . Soybeans are coated before planting. The specific agents and their dosages are shown in the table below.

[0057] Incidence rate = diseased strains / total number of strains.

[0058] Test results: Compared with seeds coated with a single agent, the overall control effect of the present invention is more obvious. Especially in formulation 2, when the wetting and dispersing agent is Morwet D-500, At l ox4913, and the film-forming agent is SYFMA001, it helps to exert the efficacy of the drug.

[0059] Table 5: Field efficacy of 14% suspension seed coating agent on soybean

[0060]

[0061] Example 6: Pyraclostrobin + Metalaxyl-M + Difenoconazole Seed Treatment Microcapsule Suspension-Suspension Concentrate

[0062] First, mix pyraclostrobin into microcapsule suspension and metalaxyl + difenoconazole % seed treatment suspension according to the proportion.

[0063] Pyraclostrobin microcapsule suspension composition ratio:

[0064] Pyraclostrobin technical: 15-25%, 200# solvent oil: 20-27%, oil phase capsule material: 1-5%, sodium salt of lignin sulfonate: 1-3%, defoamer: 0.2-1%, sulfonated aromatic polymer sodium salt: 1-5%, phosphate ester additive: 1-5%, preservative: 0.2%, 2% xanthan gum aqueous solution: 8-12%, water phase capsule material: 1-5%, deionized water: supplement

[0065] Preparation method:

[0066] Preparation method of microcapsule suspension:

[0067] Oil phase mixing: according to the type and amount of each component in the formula, put 200# solvent oil and pyraclostrobin technical into the oil phase kettle, stir and heat to 60±2 degrees Celsius, add oil capsule and stir for 10 minutes until it is evenly mixed for use;

[0068] Water phase mixing: according to the type and amount of each component in the formula, add the deionized water, sodium lignin sulfonate and defoamer required for production into the high shear stirring kettle, turn on the high shear machine, stir evenly, turn off the stirring, and heat to 58±2 degrees Celsius;

[0069] Shearing: Set the shearing machine speed to 40-50 Hz, slowly add the oil phase into the water phase for shearing, gradually increase the shearing machine speed to 60 Hz to stir into capsules, start timing after the oil phase is added, and the shearing time is 60S; the particle size is controlled below 10μm, pay attention to uniformity during shearing, and control the shearing machine height;

[0070] Adding aqueous capsule material: transfer the material from the high shear reactor to the finished product stirring reactor, start stirring, and heat to 62±2 degrees Celsius, add the aqueous capsule material and drip it over 5-10 minutes, add the sulfonated aromatic polymer sodium salt and phosphate ester additive one and a half hours after adding the aqueous capsule material, maintain the temperature and stir for 2 hours; continue stirring until stirring for 2 hours, then add the required preservatives and thickeners, and inject tap water into the jacket to replace the hot water.

[0071] Mixing and stirring: Add the required preservatives and thickeners, inject tap water into the jacket to replace hot water and stir for 40 minutes.

[0072] Inspection: The product in the mixing kettle is sampled and inspected. If it passes the inspection, it is ready to enter the mixing process. If it fails, it needs to be returned for reprocessing.

[0073] Metalaxyl-M + Pyraclostrobin seed treatment suspension

[0074] Metalaxyl original drug: 1-5%, difenoconazole original drug: 1-5%, anionic aryl ether phosphate dispersant: 2-6%, anionic aryl ether phosphate dispersant: 2-6%, defoaming agent: 0.1-1%, preservative: 0.2-0.5%, propylene glycol: 5-10%, 2% xanthan gum aqueous solution: 5-12%, red color paste: 5-10%, film-forming agent: 2-6%, deionized water: make up.

[0075] Seed treatment suspension part:

[0076] Mixing and shearing: according to the type and amount of each component in the formula, add the measured deionized water, anionic aryl ether phosphate dispersant, anionic aryl ether phosphate dispersant, defoamer, preservative and propylene glycol into the high shear reactor in turn, stir for 1 minute until uniform, then add the measured metalaxyl original drug and difenoconazole, shear and stir for 10 minutes until uniform.

[0077] Grinding: Turn on the cooling circulating water of the sand mill, and grind the evenly sheared material through the first, second and third stages of the sand mill. If the particle size D90 is less than 3μm, the material will be put into the storage tank. Otherwise, the material will be put into the feeding kettle for re-grinding.

[0078] Stirring: After grinding, add the measured grinding material, red color paste and film-forming agent into the finished product kettle in turn, and stir with a paddle for 30 minutes until it is uniform.

[0079] Inspection: The stirred product is sampled and tested. If it passes the inspection, it is ready to enter the mixing process. If it fails, it needs to be returned for reprocessing.

[0080] Mixed filling

[0081] Take the qualified microcapsule suspension and seed treatment suspension into a paddle-type stirring tank, stir and mix to form a seed treatment microcapsule suspension-suspension concentrate, stir for 30 minutes until evenly filtered and sampled for detection, and prepare a seed treatment microcapsule suspension-suspension concentrate with a mass concentration of 10% pyraclostrobin + metalaxyl-M + difenoconazole, including 5% pyraclostrobin, 3.3% metalaxyl-M and 1.7% difenoconazole.

[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention, rather than to limit it.

Claims

1. A seed treatment agent comprising difenoconazole, metalaxyl-M and pyraclostrobin, characterized in that: The active ingredients are composed of difenoconazole, metalaxyl-M and pyraclostrobin, and the mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 1-5:2-6:5-10.

2. The seed treatment agent according to claim 1, characterized in that The mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 1.5:5:7.5 or 1:1:1 or 1.7:3.3:

5.

3. The seed treatment agent according to claim 2, characterized in that The mass ratio of difenoconazole, metalaxyl-M and pyraclostrobin is 1.5:5:7.5 or 1.7:3.3:

5.

4. The seed treatment agent according to any one of claims 1 to 3, characterized in that The weight percentage of the active ingredient is 10-20%, including the auxiliary material ingredients.

5. The seed treatment agent according to claim 4, characterized in that The seed treatment agent is a suspension seed coating agent or a microcapsule suspension-suspension agent.

6. The seed treatment agent according to claim 4, characterized in that The auxiliary material components include one or more of a wetting and dispersing agent, a thickener, an antifreeze agent, a film former, a preservative, a warning color and water.

7. Use of the seed treatment agent according to any one of claims 1 to 6 for soybean seed treatment.

8. The use according to claim 7, characterized in that Used to control soybean fungal diseases.

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