Optical measuring device

Abstract

Provided is an optical measurement device (202) capable of suppressing the influence of heat dissipation of sample temperature adjustment mechanisms (403, 404, 405) for adjusting the temperature inside a camera obscura (207) in which a sample is disposed, effectively maintaining the inside of the camera obscura (207) at a low temperature while maintaining light-blocking performance, and capable of detecting bioluminescence and chemiluminescence of a substance with high sensitivity and high accuracy. An optical measurement device (202) is provided with: a detector (406) which has a light receiving unit (407) for receiving light generated from a sample tube (803) provided in a camera obscura (207), and which detects the generated light; a temperature adjustment block (401) which is provided around the detector (406), one part of which is provided inside the camera obscura (207), and the other part of which is provided outside the camera obscura (207); and a sample temperature adjustment mechanism (403, 404, 405) connected to a portion of the temperature adjustment block (401) provided outside the camera obscura (207).

Description

Technical Field

[0001] The present invention relates to an optical measuring device for detecting chemiluminescence and bioluminescence of a substance contained in a liquid sample with high sensitivity and high accuracy. Background Art

[0002] In pharmaceutical and beverage factories, products are manufactured in manufacturing facilities that implement a sterile environment.

[0003] In the past, in order to ensure the sterility of manufacturing facilities and products, the culture method was used for microbial testing. The culture method is a method of culturing a sample added with a culture medium at a high temperature for several days to 14 days, and visually counting the number of bacterial colonies that grow. Therefore, it takes time to obtain test results, and it is necessary to wait for the test results before shipping the product.

[0004] In this context, it is hoped to develop a rapid microbial detection method for rapid sterility determination.

[0005] One of the quick and easy methods for microbial detection is the adenosine triphosphate (ATP) bioluminescence method (hereinafter referred to as the ATP method). The ATP method uses the luminescence reaction of fireflies to convert the ATP present in the bacteria into light for measurement.

[0006] More specifically, luciferase is made to take up luciferin and ATP molecules, and the amount of light emitted when luciferin (oxyluciferin) oxidized as ATP is consumed transitions from an excited state to a ground state is measured.

[0007] At this time, the consumption of ATP molecules generates photons. The number of photons generated is proportional to the number of ATP. ATP molecules exist in living bacteria as energy sources, and by measuring the amount of light generated by ATP in the specimen, the total number of living bacteria can be estimated.

[0008] The luciferin-luciferase reaction has the best quantum efficiency (ΦBL: ≈0.5) among bioluminescence and chemiluminescence, so one cell can be detected as equivalent to hundreds of thousands of photons.

[0009] Therefore, the ATP method is a method capable of detecting light equivalent to one cell in principle.

[0010] However, in order to detect extremely small amounts (several levels) of bacteria mixed into a specimen with high sensitivity and high accuracy, it is necessary to improve the sensitivity of the luminescence measurement device.

[0011] The luminescence coefficient based on the ATP method is implemented using a kit from a certain manufacturer, and the measurement steps are briefly described as follows.

[0012] 1) Pour ATP elimination solution into the sample solution to eliminate dead bacteria other than live bacteria and ATP in the liquid. 2) Inject the ATP extract solution into the sample solution to extract ATP from the live bacteria. 3) Dispense the luminescent reagent into the sample solution. 4) Measure the luminescence amount.

[0013] In order to improve the sensitivity of luminescence measurement, the temperature of the detector needs to be kept low to reduce the noise of the measurement equipment, and the part holding the measurement equipment needs to be set as a light-shielding space to eliminate the influence of external light.

[0014] Furthermore, since luminescence is generated immediately when the luminescent reagent is dispensed into the sample container, in order to measure the accurate amount of ATP, it is desirable to carry out the steps 3) and thereafter with the sample placed in the detector.

[0015] Although the influence of external light is usually eliminated by covering the light detector and sample container with a shading structure, it is also effective to locally block the light of the luminescence detector. There are examples of using a luminometer and installing an openable shutter in front of the light-receiving surface of the light detector.

[0016] On the other hand, it is also necessary to prevent contamination from ATP, bacteria, microorganisms carried by humans, dust that absorbs ATP, etc. in the environment. It is important to automate the dispensing of reagents and the transfer of samples to the measurement location in the device to minimize human operations, while at the same time eliminating air circulation inside the device as much as possible.

[0017] Patent Document 1 describes a technique in which a shutter that is normally closed and opened during luminescence measurement is provided to prevent external light from intruding into a luminescence detection unit when luminescence measurement is not performed, thereby preventing degradation.

[0018] Furthermore, Patent Document 2 describes a technique in which a shutter unit is closed in preparation for measurement to prevent stray light from entering a photodetector, and the shutter unit is opened in a measurement state. Prior art literature Patent Literature

[0019] [Patent Document 1] Japanese Patent Laid-Open No. 7-83831 [Patent Document 2] Japanese Patent Publication No. 2008-268019 Summary of the invention Technical problem to be solved by the invention

[0020] In an analysis device that counts the number of photons using a photodetector, it is necessary to suppress the dark level of the light receiving portion to a certain level or less in order to improve the detection sensitivity.

[0021] Generally, in order to suppress the dark level of the photodetector, it is necessary to place the light receiving unit in a dark box structure and adjust the temperature to a low temperature.

[0022] When the temperature adjustment mechanism of the photodetector is disposed in a dark box structure, there are problems such as a rise in temperature in the dark box structure and a decrease in the efficiency of the enzyme reaction due to heat dissipation caused by the temperature adjustment.

[0023] In the technology described in Patent Documents 1 and 2, although content about shading is taught, there is no content about maintaining the temperature in the dark box at a low temperature, and the problem of reduced enzyme reaction efficiency due to heat dissipation caused by the temperature control mechanism is not recognized and is not taken into consideration.

[0024] The object of the present invention is to realize an optical measuring device which can suppress the influence of heat dissipation of a temperature regulating mechanism for regulating the temperature inside a dark box in which a sample is arranged, effectively keep the inside of the dark box at a low temperature while maintaining the light-shielding performance, and can detect the bioluminescence and chemiluminescence of a substance with high sensitivity and high precision. Technical means for solving technical problems

[0025] In order to achieve the above-mentioned object, the present invention is configured as follows.

[0026] The optical measuring device of the present invention includes: a detector having a light receiving portion for receiving light generated from a sample tube arranged in a dark box and detecting the generated light; a temperature adjustment block arranged around the detector; and a sample temperature adjustment mechanism connected to the temperature adjustment block, a part of the temperature adjustment block being arranged in the dark box and another part being arranged outside the dark box, the sample temperature adjustment mechanism being connected to the part of the temperature adjustment block arranged outside the dark box. Effects of the Invention

[0027] According to the present invention, an optical measuring device can be realized, which can suppress the influence of heat dissipation of a temperature regulating mechanism that regulates the temperature inside a dark box where a sample is placed, effectively keep the inside of the dark box at a low temperature while maintaining the light-shielding performance, and can detect the bioluminescence and chemiluminescence of a substance with high sensitivity and high precision. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a diagram showing the overall structure of the microorganism rapid detection device according to the embodiment. Figure 2 It is a diagram showing the overall structure of the microorganism rapid detection device according to the embodiment. Figure 3 This is a diagram showing a schematic internal structure of a rapid microorganism detection device according to an embodiment. Figure 4 It is a diagram showing a schematic structure of a photodetector unit according to an embodiment. Figure 5 It is a simplified structural diagram of the sample tube conveying mechanism. Figure 6 This is a diagram showing a schematic structure of a reagent dispensing machine. Figure 7 It is a diagram showing a schematic structure of a reagent rack. Figure 8 FIG. 1 is a diagram schematically showing a light shielding structure of a photodetector unit. Fig. 9 It is a diagram showing a schematic configuration of a light receiving section of a photodetector unit according to an embodiment. Fig.10 This is a flowchart for explaining a typical example of luminescence measurement in the embodiment. Fig.11 It is a diagram showing a schematic structure of a modified example of the photodetector unit of the embodiment. DETAILED DESCRIPTION

[0029] Hereinafter, embodiments of the present invention will be described with reference to the drawings.

[0030] However, this embodiment is only an example for realizing the present invention and is not intended to limit the present invention. In addition, in each of the accompanying drawings, the same reference numerals are used to mark common structures. In addition, in the description of the following embodiments, sample has the same meaning as test material. [Example]

[0031] Figure 1 1 is a diagram showing the overall structure of a microorganism rapid detection device 100 having a photodetector unit 202 (described later) as an optical measurement device according to an embodiment. The microorganism rapid detection device 100 is a system including a control PC 103, and is powered by a power cable 101. The microorganism rapid detection device 100 is connected to the control PC 103 via a communication cable 102.

[0032] The microorganism rapid detection device 100 includes a system water drawer 105 for loading system water for liquid delivery and cleaning of the dispenser, a waste liquid drawer 104 for accessing a waste liquid section into which the cleaning drainage of the dispenser flows, a reagent door 106 for loading a luminescent reagent into the microorganism rapid detection device 100, and a sample tube 803 ( Figure 8107 of the sample door 107 of the microorganism rapid detection device 100. Although not shown, each door is configured as a switch door provided with a locking mechanism or a structure that can be pulled out by a slide rail or the like.

[0033] Figure 2 1 is a diagram showing a schematic structure of a rapid microorganism detection device 100 .

[0034] exist Figure 2 In the embodiment, the microorganism rapid detection device 100 includes a dark box 207 whose interior is a dark box structure by a light shielding structure; and a housing 108 surrounding a portion that is not a dark box structure. The dark box 207 includes a front panel 206, and the front panel 206 has a reagent door 106 and a sample door 107. The light shielding structure is formed by closing the reagent door 106 and the sample door 107. In addition, the reagent door 106 and the sample door 107 are external air intake parts that inhale external air.

[0035] Though not shown, in the door portion with light-shielding structure, the concavo-convex structure is set on the door opening and black anodizing is carried out. In addition, by inserting the sealing member made of resin in the periphery of the front panel 206, the structure of light-shielding between the front panel 206 and the housing is formed, thereby constituting the dark box 207.

[0036] On the other hand, the system water drawer 105, waste liquid drawer 104, liquid delivery syringe 204, and control substrate and power supply required for device operation are arranged outside the structure of the dark box 207. The piping from the liquid delivery syringe 204 to the inside is connected by the bulkhead piping 205.

[0037] Although not shown, the wiring harness connection to the dark box 207 is a light-shielding connection substrate 201 which is a substrate having a relay connector, and is connected in a light-shielding manner by sandwiching a light-shielding seal, thereby connecting from a substrate / power supply arranged outside the dark box structure to a unit arranged inside the dark box.

[0038] The reagents for determination are placed on the reagent rack 203 by the reagent door 106. As described below, the reagent rack 203 includes a reagent temperature adjustment mechanism 805 and is temperature-adjusted to a constant temperature. In addition, the sample tube 803 is placed on the rack and is placed on the sample rack mechanism 301 (such as the sample rack 302) by the sample door 107. Figure 3 shown).

[0039] Figure 3 1 is a diagram showing a schematic internal structure of the rapid microorganism detection device 100 .

[0040] exist Figure 3In the figure, the rapid microbial detection device 100 includes a reagent rack 203 for storing reagents, a light detector unit 202 for measuring, a sample rack mechanism 301 for holding a sample tube 803 for containing a sample, a chuck arm 303 for transporting the tube rack inside a dark box 207, and a dispenser 302 for dispensing reagents.

[0041] Highly sensitive detection is generally achieved by using a photomultiplier tube (hereinafter referred to as PMT) as the photodetector unit 202 .

[0042] However, when the sensitivity of a PMT is not required, a semiconductor element such as a photodiode may be used. In this specification, the case of using a PMT is described.

[0043] The microorganism rapid detection device 100 includes a chuck arm 303 for transferring a sample tube 803 disposed in a sample rack mechanism 301 .

[0044] Figure 5 This is a simplified structural diagram of the sample tube transport mechanism. Figure 5 In the process, the sample tube 803 is gripped by the chuck arm 303 and the tube to be measured is transferred to the photodetector unit 202.

[0045] Although not shown, the sample rack mechanism 301 includes a Y drive unit that can be driven in the Y-axis direction, and these drive mechanisms can process a plurality of sample tubes 803. As the drive unit of the sample rack mechanism 301, for example, a drive mechanism based on an actuator or a pulley can be used.

[0046] The chuck arm 303 includes a tube clamping portion 504 for clamping the sample tube 803 and a chuck opening and closing mechanism 503 for opening and closing the tube clamping portion 504. As the chuck opening and closing mechanism 503, for example, an opening and closing mechanism based on the rotation of a rotary solenoid or an opening and closing mechanism based on a push-pull solenoid can be used.

[0047] Although not shown, the chuck gripping portion 504 includes two or more claws and is driven by the chuck opening and closing mechanism 503 to grip the sample tube 803. In addition, the chuck arm 303 includes an X-drive portion 501 that can be driven in the X-axis direction and a Z-drive portion 502 that can be driven in the Z direction, and by driving in the X direction and the Z direction, the sample tube 803 set in the tube rack is transferred to the photodetector unit 202. As the drive system of the chuck arm 303, for example, a drive mechanism based on an actuator or a pulley can be used.

[0048] Figure 6 This is a diagram showing a schematic structure of a reagent dispensing machine.

[0049] exist Figure 6In the embodiment, the reagent is sucked and discharged to the sample tube 803 by the dispensing nozzle 603. The dispensing nozzle 603 is connected to the syringe 604, the electromagnetic valve 606, the electromagnetic valve 607, and the system water 605 through the pipe 608. The dispensing nozzle 603 includes an X-drive unit 602 that can be driven in the X-axis direction and a Z-drive unit 601 that can be driven in the Z direction. By driving in the X direction and the Z direction, the dispensing action from the reagent loaded on the reagent rack 203 to the sample tube 803 set in the light detector unit 202 is implemented.

[0050] As a driving system, for example, a driving mechanism based on an actuator or a pulley can be used. In addition, although the dispensing machine is not shown, the dispensing nozzle 603 moves to the cleaning port, and the internal and external cleaning of the dispensing nozzle 603 is performed using system water 605. The waste liquid after cleaning is discharged into a waste liquid bottle provided in the waste liquid drawer 104 arranged outside the dark box 207 through a pipe.

[0051] Figure 7 2 is a diagram showing a schematic structure of the reagent rack 203 .

[0052] exist Figure 7 In the embodiment, the reagent rack 203 is composed of a support 701 that supports a reagent holder 703 that supports a reagent tube 702, and a reagent temperature adjustment mechanism 805. The temperature of the support 701 is adjusted to a constant temperature by the reagent temperature adjustment mechanism 805. Figure 7 2 shows a configuration example of the temperature adjustment mechanism 805 . The reagent temperature adjustment mechanism 805 includes a Peltier element 710 , a heat sink 707 , a heat sink block 709 , and an air cooling fan 708 .

[0053] The support 701 of the reagent rack 203 is arranged in the dark box 207. On the other hand, a radiator 707 and an air cooling fan 708 for heat dissipation are arranged outside the structure of the dark box 207. A light shielding block 706 is arranged between the inside of the dark box 207 and the outside radiator 707 to shield the dark box 207. The light shielding block 706 includes a light shielding member 705 and a middle plate 704 arranged on the inner side of the dark box 207.

[0054] Figure 4 2 is a diagram showing a schematic structure of the photodetector unit 202 .

[0055] exist Figure 4 In the embodiment, the photodetector unit 202 includes a detector 406, a temperature adjustment block 401 provided to surround the detector 406, a light shielding connection portion 402, and a sample temperature adjustment mechanism (a cooling fan 403, a heat sink 404, and a Peltier element 405).

[0056] The light receiving unit 407 of the detector 406 is disposed in the dark box 207 so as to face the sample tube 803 , and the sample temperature adjustment mechanism is disposed outside the structure of the dark box 207 . Figure 4 4 shows a configuration example of a sample temperature adjustment mechanism, which includes a Peltier element 405, a heat sink 404, and an air cooling fan 403, and controls a temperature adjustment block 401 surrounding a detector 406 to a constant temperature.

[0057] The dark box 207 is surrounded by a housing 108 having an external air intake portion, and the temperature adjustment block 401 is provided across the dark box 207 and the interior of the housing 108 which is the exterior of the dark box 207 .

[0058] Figure 8 2 is a diagram schematically showing a light shielding structure in the photodetector unit 202 .

[0059] exist Figure 8 In the embodiment, the light shielding connection part 402 forms a light shielding structure by sandwiching the light shielding member 801 in the opening part provided in the middle plate 704, and the middle plate 704 constitutes the dark box 207. The light shielding connection part 402 and the connection part of the temperature adjustment block 401 also have a light shielding structure that sandwiches the light shielding member 801. As the light shielding member 801, a resin material, a sealing member, etc. can be used. In addition, in addition to the structure of sandwiching the light shielding member 801, the part for performing the light shielding connection can also be set as a concave-convex structure to perform light shielding.

[0060] Fig. 9 FIG. 4 shows a schematic structure of the light receiving part of the light detector unit 202. The temperature adjustment block 401 includes a sample tube holding part 401h for holding the sample tube 803. Fig. 9 As shown, the sample tube holding part 401h forms a conical inner space (conical part) and holds the sample tube 803 in the inner space. By holding the sample tube 803 in the inner space, the temperature stability of the sample contained in the sample tube 803 is improved.

[0061] The inner wall of the conical part of the sample tube holding part 401h uses mirror reflection to recover light scattered in a direction different from the light receiving part of the detector 406. In order to realize mirror reflection, a member subjected to polishing or electroplating of a metal material is used. By using aluminum or the like as the metal material, stable reflection efficiency can be obtained.

[0062] Furthermore, the shape of the portion serving as the holding tube may be not only a conical shape but also another shape such as a semicircle.

[0063] like Fig. 9As shown, the sample tube 803 provided on the detector 406 is provided above the light receiving portion 407 of the light detector unit 202 for detection. The closer the distance between the sample tube 803 and the light receiving portion 407 of the detector 406 is, the higher the detection sensitivity is. However, the sample tube 803 is made of plastic in most cases and may be charged with static electricity.

[0064] Therefore, when the sample tube 803 and the light receiving unit 407 are brought close to each other, there is a possibility of discharge. Due to the discharge or the like, the detector 406 may not be able to perform accurate measurement.

[0065] Therefore, a glass plate 802 is provided between the detector 406 and the sample tube 803. A material having sufficient transmittance in the visible region, such as a quartz plate, is used as the glass plate 802. A fluorescent filter having a specific wavelength band in the wavelength portion of the luminescent reaction may also be selected.

[0066] Fig.10 This is a flowchart for explaining a typical example of luminescence measurement according to one embodiment of the present invention.

[0067] exist Fig.10 In the embodiment, after the microorganism rapid detection device 100 and the software in the control PC 103 are started, the reagent door 106 is opened (step S01), and the reagent holder 703 provided with the luminescent reagent is set on the bracket 701 (step S02). Then, after the reagent holder 703 is set on the bracket 701, the reagent door 106 is closed (step S03).

[0068] Next, the sample door 107 is opened (step S04), and a tube rack with a tube set therein is set on the sample rack mechanism 301, and the tube is loaded with a measurement sample (step S05). After the tube rack is set on the sample rack mechanism 301, the sample door 107 is closed (step S06). The measurement conditions are input through the control software, and the measurement start button of the control PC 103 is pressed.

[0069] After the measurement starts, the sample tube 803 is transferred from the sample rack mechanism 301 to the photodetector unit 202 by the chuck arm 303 (step S07). The luminescent reagent is dispensed from the reagent tube 702 set in the reagent holder 703 into the transferred sample tube 803 by the dispenser 302 (step S08).

[0070] After the reagent is dispensed into the sample tube 803, light emission is generated, and the number of light emission is measured by the detector 406 (step S09).

[0071] After measuring the luminescence count, the sample tube 803 is transferred to the sample rack mechanism 301 by the chuck arm 303 (step S10). In addition, the dispenser 302 moves to the cleaning port and uses the system water 605 to clean the dispensing nozzle 603 (step S11). When there are multiple samples, the sample tube 803 to be measured next is transferred, and luminescence measurement is performed in sequence (steps S07 to S11).

[0072] After all samples are measured, the reagent door 106 is opened (step S12) and the luminescent reagent is taken out (step S13). After the luminescent reagent is taken out, the reagent door 106 is closed (step S14) and the sample door 107 is opened (step S15). The sample tube rack is taken out (step S16), and then the sample door 107 is closed (step S17) and the process ends.

[0073] The acquired data of the luminescence number is sent to the control PC 103, where the conversion from the luminescence number to the ATP concentration is performed using a pre-created calibration curve. In the software involved in the present invention, there are multiple calibration curves, and the conversion to the ATP concentration is performed using the selected calibration curve, and the measurement data and the calibration curve information are stored in association.

[0074] As described above, the optical measuring device 202 of one embodiment of the present invention includes: a detector 406 having a light receiving portion 407 that receives light generated from a sample tube 803 disposed in a dark box 207 and detects the generated light; a temperature adjustment block 401 disposed around the detector 406, a portion of the temperature adjustment block 401 being disposed in the dark box 207 and another portion being disposed outside the dark box 207; and temperature adjustment mechanisms 403, 404, 405 connected to the portion of the temperature adjustment block 401 disposed outside the dark box 207.

[0075] A portion of the temperature adjustment block 401 covers the light receiving portion 407 of the light detector unit 202 in the dark box 207, and the other portion of the temperature adjustment block 401 is arranged outside the dark box 207. A cooling fan 403, a radiator 404 and a radiator 404 serving as a temperature adjustment mechanism are provided on the portion of the temperature adjustment block 401 arranged outside the dark box 207.

[0076] Therefore, according to one embodiment of the present invention, an optical measuring device 1 can be realized, which can suppress the influence of heat dissipation of a temperature regulating mechanism that regulates the temperature inside a dark box 207 in which a sample is placed, effectively maintain the inside of the dark box 207 at a low temperature while maintaining the light-shielding performance, and can detect the bioluminescence and chemiluminescence of a substance with high sensitivity and high precision.

[0077] In addition, according to one embodiment of the present invention, since the Peltier element 710 that keeps the temperature of the bracket 701 that holds the reagent holder of the reagent tube 702 constant is arranged in the dark box 207, and the radiator 707 and the cooling fan 708 of the heat sink 709 connected to the Peltier element 710 are arranged outside the dark box 207, the reagent in the dark box 207 can be effectively maintained at a constant temperature.

[0078] In addition, in the above embodiment, if Figure 4 , Figure 8 , Fig. 9 As shown in FIG. 1 , the temperature control block 401 surrounds the light receiving portion 407 of the light detector unit 202. Fig.11 As shown, the temperature adjustment block 401 may also be configured not to surround the light receiving portion 407 .

[0079] exist Fig.11 In the illustrated modification, the light receiving section 407 is surrounded by the sample tube support member 804 , the glass plate 802 is supported, and the sample tube 803 is also held. Fig. 9 The schematic structure of the light receiving part of the photodetector is shown in . The temperature adjustment block 401 includes a sample tube holding part 401h for holding the sample tube 803 .

[0080] exist Fig.11 In the example shown, Fig. 9 In the example shown, the sample tube support member 804 is formed with a conical inner space (conical portion), and holds the sample tube 803 in the inner space. By holding the sample tube 803 in the inner space, the temperature stability of the sample contained in the sample tube 803 is improved. Description of symbols

[0081] 100 Microbial rapid detection device, 101 Power cable, 102 Communication cable, 103 Control PC, 104 Waste liquid drawer, 105 System water drawer, 106 Reagent door (external air intake part), 107 Sample door (external air intake part), 108 Shell, 201 Light shielding connection substrate, 202 Photodetector unit (optical measurement device), 203 Reagent rack, 204 Liquid delivery syringe, 205 Bulkhead piping, 206 Front panel, 207 Dark box, 301 Sample rack mechanism, 302 Dispensing machine, 303 Chuck arm, 401 Temperature adjustment block, 401h Sample tube holding part, 402 Light shielding connection part, 403 Air cooling fan, 404 Radiator, 405 Pearl Peltier element, 406 detector, 407 light receiving unit, 501X drive unit, 502Z drive unit, 503 chuck opening and closing mechanism, 504 tube clamping unit, 601Z drive unit, 602X drive unit, 603 dispensing nozzle, 604 syringe, 605 system water, 606 solenoid valve 1, 607 solenoid valve 2, 608 piping, 701 bracket, 702 reagent tube, 703 reagent holder, 704 middle plate, 705 shading member, 706 shading block, 707 radiator, 708 air cooling fan, 709 heat sink, 710 Peltier element, 801 shading member, 802 glass plate, 803 sample tube, 804 sample tube supporting member, 805 reagent temperature adjustment mechanism.

Claims

1. An optical measuring device, characterized in that: include: a detector having a light receiving portion for receiving light generated from a sample tube disposed in the dark box and detecting the generated light; a temperature regulating block, the temperature regulating block being arranged around the detector; as well as a sample temperature regulating mechanism connected to the temperature regulating block, A portion of the temperature adjustment block is disposed in the dark box, and the other portion is disposed outside the dark box. The sample temperature adjustment mechanism is connected to the portion of the temperature adjustment block disposed outside the dark box.

2. The optical measuring device according to claim 1, wherein The temperature adjustment block is provided in the dark box so as to cover the outer periphery of the light receiving unit.

3. The optical measuring device according to claim 2, wherein The temperature adjustment block includes a sample tube holding portion that holds the sample tube in the dark box.

4. The optical measuring device according to claim 1, wherein The dark box is surrounded by a housing having an external air intake portion, and the temperature adjustment block is provided across the dark box and the interior of the housing, which is the exterior of the dark box.

5. The optical measuring device according to claim 1, wherein The dark box is provided with: a conveying mechanism for the sample tube; and A dispensing mechanism for dispensing reagents.

6. The optical measuring device according to claim 1, wherein In the dark box, a reagent temperature adjustment mechanism is provided, a part of which is provided inside the dark box, and the other part is provided outside the dark box. The reagent temperature adjustment mechanism includes a Peltier element, a radiator, a heat sink and a cooling fan, and the radiator and the cooling fan are provided outside the dark box.

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