Container for preparing carbon monoxide delayed encephalopathy animal model

Abstract

The invention discloses a container for preparing a carbon monoxide delayed encephalopathy animal model, and relates to the technical field of biomedical experiment equipment, the container comprises a rectangular box body, the interior of the box body is a central control space, the periphery and the bottom of the box body are fixedly connected plates, the top of the box body is provided with an upper cover, one side of the box body is provided with a turbulent flow fan, and the other side of the box body is provided with an air inlet. The turbulent flow fan is fixedly connected with the box body, a second turbulent flow fan is installed on the side, away from the turbulent flow fan, of the box body, an air inlet valve is arranged on a plate between the turbulent flow fan and the second turbulent flow fan, an exhaust valve is arranged on a plate on the side, away from the air inlet valve, of the box body, and standby air valves are arranged on one side of the turbulent flow fan and one side of the second turbulent flow fan. A standby threaded opening is formed in the bottom of the exhaust valve. Compared with the prior art, the container has the advantages of high-precision control, uniform gas mixing, flexibility, expandability, high efficiency, automation, safety and the like, and provides powerful support for scientific research.

Description

Technical Field

[0001] The invention relates to the technical field of biomedical experimental equipment, in particular to a special container for preparing a Delayed Encephalopathy After Carbon Monoxide Poisoning (DEACMP) animal model. Background Art

[0002] Carbon monoxide delayed encephalopathy is a serious neurological disease with a complex pathogenesis and diverse clinical manifestations. In order to deeply study the pathophysiological process of the disease and explore effective prevention and treatment strategies, it is crucial to construct a stable and reliable animal model. However, the existing animal model preparation containers have many shortcomings in terms of gas mixing uniformity, concentration control accuracy, and automation level, which limits the quality and efficiency of model preparation.

[0003] In the prior art, only the patent with publication number CN113707319A involves the prediction model of delayed encephalopathy caused by carbon monoxide poisoning. However, the article only gives the relevant experimental steps and does not explain what device is used to achieve it. In addition, the test subjects are humans, which has a higher degree of danger. It is better to use an animal model. However, this patent is not suitable for animal model testing. Summary of the invention

[0004] The technical problem to be solved by the present invention is that, in view of the shortcomings of the prior art, the present invention proposes a high-precision, multifunctional carbon monoxide delayed encephalopathy animal model preparation container, which achieves precise control of key parameters such as experimental gas concentration, flow rate, mixing uniformity, etc. through optimized structural design, advanced control technology and flexible experimental configuration, thereby improving the accuracy and reliability of model preparation.

[0005] In order to solve the above technical problems, the technical solution provided by the present invention is: a container for preparing an animal model of delayed encephalopathy caused by carbon monoxide, comprising a box body, wherein the box body is a rectangular box body, the interior of the box body is a hollow space, the four sides and the bottom of the box body are fixedly connected plates, an upper cover is provided on the top of the box body, a spoiler fan is installed on one side of the box body, the spoiler fan and the box body are fixedly connected, a spoiler fan 2 is installed on the side of the box body away from the spoiler fan, an air intake valve is provided on the plate between the spoiler fan and the spoiler fan 2, an exhaust valve is provided on the plate on the side of the box body away from the air intake valve, a spare air valve is provided on one side of the spoiler fan and the spoiler fan 2, and a spare threaded port is provided at the bottom of the exhaust valve.

[0006] As an improvement, the spoiler fan and the spoiler fan 2 are symmetrically distributed along the center line of the box. The spoiler fan is located at the high part of the box plate, and the spoiler fan 2 is located at the low part of the box plate. The two fans are staggered, so that they can provide air circulation for the rat cages in the two groups of rat experiments respectively.

[0007] As an improvement, there are three intake valves, which are evenly spaced along the central axis of the box. The intake valves are located at the bottom of the box. The intake valves are used to provide gas and are connected to the solenoid valve connected to the air inlet pipeline through a single-chip control circuit to ensure a constant gas concentration in the box.

[0008] As an improvement, there are three exhaust valves, which are evenly spaced along the central axis of the box. The air inlet valve is set high up in the box. The high exhaust valve can ensure that the experimental gas can be discharged from the top, and will not fail to reach the top because the air inlet and exhaust are at the bottom.

[0009] As an improvement, the centers of the spare air valves on both sides are collinear and are both located on the central axis of the box. Since the height and position of the spare air valves correspond and are located in the middle of the box, there is no situation where the inlet and exhaust valves are low in and low out.

[0010] As an improvement, a sealing ring is provided at the edge of the upper cover and quick clips are provided around it for easy locking. While the sealing ring ensures airtightness, the quick clips can also be quickly fixed, making it easy to move the box.

[0011] As an improvement, the internal size of the box can stack two rat cages. At the same time, if rat cages are not placed, hollow baffles can be placed, and the double layer ensures the modeling efficiency.

[0012] As an improvement, the spare threaded port is a M20*1.5 standard thread, which is used to connect a gas concentration monitor. The gas concentration monitor is connected to a solenoid valve connected to the air inlet pipeline through a single-chip microcomputer control circuit.

[0013] As an improvement, solenoid valves are installed on both the intake valve and the exhaust valve to facilitate PLC control. The setting of the solenoid valve can facilitate opening and closing while ensuring air tightness.

[0014] As an improvement, one-way valves are installed on the spare gas valves to prevent gas backflow. There are one-way valves on both sides, one side in and one side out, to prevent gas backflow and cause inaccurate results.

[0015] The advantages of the present invention compared with the prior art are:

[0016] High-precision control: Through the application of high-precision flow control valves, pressure sensors, temperature sensors and PLC systems, precise control of key parameters such as experimental gas concentration, flow, pressure, temperature, etc. is achieved, improving the accuracy and reliability of model preparation.

[0017] Uniform gas mixing: The high and low staggered layout of the dual spoiler fans and the precise intake valve design achieve uniform mixing and full distribution of the gas in the box, ensuring uniform exposure of the experimental gas in the animal model.

[0018] Flexibility and scalability: The scalable design of spare gas valves, spare threaded ports and PLC system enables the container to adapt to different experimental needs and future technological upgrades, providing more possibilities and flexibility for scientific research.

[0019] Efficiency and automation: The application of PLC system realizes the automation and intelligence of the experimental process, reduces manual intervention and errors, and improves experimental efficiency and accuracy.

[0020] Safety: The design of the one-way valve and sealing ring ensures the safety and stability of the experimental process and prevents potential safety hazards such as gas backflow and leakage. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a schematic diagram of the structure of a container used for preparing an animal model of delayed encephalopathy induced by carbon monoxide.

[0022] Figure 2 It is a blood gas analysis graph.

[0023] As shown in the figure: 1. Box body, 2. Top cover, 3. Turbine fan, 4. Turbine fan 2, 5. Inlet valve, 6. Exhaust valve, 7. Spare air valve, 8. Spare threaded port. DETAILED DESCRIPTION

[0024] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0025] In the specific implementation of the present invention, the container body of the present invention is a rectangular box body 1, the interior of which is hollow, and the surroundings and the bottom are fixedly connected with high-strength, corrosion-resistant plates to ensure the structural stability and durability of the container. The top of the box body 1 is provided with an upper cover 2, and a high-sealing silicone sealing ring is installed at the edge of the upper cover. Quick clamping and locking devices are provided around, which facilitates experimental operation while ensuring the airtightness of the box body.

[0026] On one side of the box 1, a high-performance spoiler fan 3 is installed, and on the other side, a spoiler fan 2 4 is installed. The two are symmetrically distributed along the center line of the box, but at different heights, forming a well-arranged layout. This design not only promotes the flow and mixing of gas in the box, but also improves the uniform distribution of gas, ensuring that the experimental gas is fully mixed and evenly distributed in the box.

[0027] Three precise air intake valves 5 are provided on the plate between the spoiler fan 3 and the spoiler fan 2 4. They are evenly spaced along the central axis of the box 1 and are located at a lower position of the box. The air intake valve 5 uses a high-precision flow control valve, which can accurately adjust the flow and concentration of the experimental gas to meet the experimental requirements. At the same time, the air intake valve is also provided with a pressure sensor and a temperature sensor. The pressure sensor uses the Honeywell MSP300 series, and the temperature sensor is a conventional thermistor temperature sensor, which monitors the gas pressure and temperature in real time to ensure the stability and accuracy of the experimental conditions.

[0028] On the plate far from the side of the intake valve 5, there are three exhaust valves 6, which are also equally spaced along the central axis, but located at a higher position in the box. The exhaust valve 6 uses a high-performance exhaust device, which can quickly exhaust the exhaust gas and maintain the freshness and renewal speed of the gas in the box. At the same time, the exhaust valve is also provided with a gas concentration monitor interface, which is convenient for real-time monitoring of the gas concentration in the box to ensure the consistency of the experimental conditions.

[0029] In addition, a spare threaded port 8 is provided at the bottom of the exhaust valve 6. The threaded port adopts the M20*1.5 standard, which is convenient for connecting additional gas concentration monitors or other experimental equipment, thereby increasing the flexibility and scalability of the container.

[0030] In order to further realize automatic control, solenoid valves are installed on the inlet valve 5 and the exhaust valve 6. The solenoid valves are 2L170-10 type solenoid valves. These solenoid valves can be accurately controlled by PLC programmable logic controllers. The PLC system can automatically adjust the inlet and outlet flow and concentration of the gas according to the preset experimental parameters and procedures, realizing the automation and intelligence of the experimental process.

[0031] At the same time, a one-way valve is installed on the backup gas valve 7 to prevent gas backflow and ensure the stability and safety of the experimental process. The design of the backup gas valve 7 also takes into account future expansion needs, and can be connected to additional gas input pipelines or equipment to provide more flexibility and possibilities for the experiment.

[0032] In addition, the internal dimensions of the box 1 are reasonably designed, and two rat cages can be stacked to meet the needs of preparing multiple animal models at the same time. This design not only improves the experimental efficiency, but also facilitates the simultaneous observation and comparison of multiple animal models, providing more data support for scientific research.

[0033] Working principle of the present invention: The working principle of the high-precision, multifunctional carbon monoxide delayed encephalopathy animal model preparation container is mainly based on the following key points:

[0034] 1. Gas Control and Mixing

[0035] High precision flow control valve:

[0036] The air inlet valve on the container adopts a high-precision flow control valve, which can accurately adjust the flow and concentration of experimental gases such as carbon monoxide entering the container.

[0037] This precise control ensures that the animal model is exposed to a stable and preset gas environment during preparation.

[0038] Dual spoiler fan design:

[0039] The container is equipped with dual turbulence fans, whose high and low staggered layout promotes the flow and mixing of gas.

[0040] By agitating the gas within the container, the dual turbulence fans ensure uniform distribution of the experimental gas within the container, thereby improving the consistency and accuracy of animal model preparation.

[0041] 2. Automation and Intelligent Control

[0042] PLC system:

[0043] The container realizes automation and intelligent control of the experimental process through the PLC system.

[0044] The PLC system can automatically adjust the gas inlet and outlet flow, concentration and other related parameters according to the preset experimental parameters and procedures.

[0045] Data collection:

[0046] Gas flow, concentration, pressure, temperature and other parameters are collected through sensors and transmitters, and these analog signals are converted into digital signals that can be recognized by PLC.

[0047] Sensors and transmitters are usually connected to the analog input module AI of the PLC.

[0048] Data processing:

[0049] After receiving the digital signal, the PLC processes the data according to the preset program and algorithm.

[0050] For example, the instantaneous flow rate can be calculated using a general formula for the differential pressure signal, or the flow value can be corrected using a specific compensation formula.

[0051] Control strategy:

[0052] According to the experimental parameters and program requirements, the PLC calculates the target values ​​such as gas flow rate and concentration that need to be adjusted.

[0053] By comparing the actual measured value with the target value, the PLC determines the control action that needs to be performed.

[0054] For example, if the actual flow rate is less than the target flow rate, the PLC might output a signal to open a larger solenoid valve to increase the flow rate.

[0055] Output control signal:

[0056] PLC outputs control signals through digital output module DO or analog output module AO.

[0057] These signals are used to control the actions of output devices such as solenoid valves, pneumatic valves, and frequency converters.

[0058] For example, the PLC can output a pulse signal to control the switching time of the solenoid valve, thereby adjusting the flow of the gas.

[0059] This automated control reduces manual intervention and errors, and improves experimental efficiency and accuracy.

[0060] Sensor monitoring:

[0061] The inside of the container is equipped with sensor equipment such as pressure sensors, temperature sensors and gas concentration monitors.

[0062] These sensors can monitor the gas environment parameters in the container in real time to ensure the stability and accuracy of experimental conditions.

[0063] At the same time, sensor data can also provide feedback to the PLC system for more precise adjustment and control.

[0064] 3. Safety and Reliability

[0065] One-way valve and sealing ring:

[0066] The backup gas valve on the container adopts a one-way valve design to prevent safety hazards such as gas backflow and leakage.

[0067] At the same time, highly airtight silicone sealing rings are used to seal the various parts of the container to ensure the airtightness and safety of the container.

[0068] Emergency stop device:

[0069] Although not explicitly mentioned in the specific implementation scheme, in order to ensure the safety of the experimental process, the container can also be equipped with an emergency stop device, and the emergency stop button uses a normally closed contact to ensure that the circuit can be reliably disconnected when the emergency stop button is pressed.

[0070] When an abnormal situation occurs during the experiment, the operator can quickly cut off the gas and power supply through the emergency stop device to prevent the accident from expanding.

[0071] IV. Experimental Procedure and Animal Model Preparation

[0072] Experimental gas input:

[0073] At the beginning of the experiment, the operator inputs carbon monoxide gas with a preset concentration into the container through a high-precision flow control valve.

[0074] At the same time, the PLC system starts working and automatically adjusts the gas flow and concentration according to the preset experimental parameters and procedures.

[0075] Animal Model Exposure:

[0076] The animal model to be prepared, such as a rat, is placed in an animal cage in the container.

[0077] Animals are exposed to a preset experimental gas environment for a certain period of time to simulate the pathophysiological process of carbon monoxide poisoning.

[0078] Data recording and analysis:

[0079] During the experiment, the sensor monitors and records the gas environment parameters in the container, including carbon monoxide concentration and oxygen percentage, in real time.

[0080] After the experiment, the operator can collect relevant data of the animal model, such as behavioral performance, physiological indicators, etc., for analysis and comparison.

[0081] Table 1 Rat blood gas analysis results

[0082]

[0083] These data can be used to evaluate the effectiveness of animal model preparation and the effects of carbon monoxide poisoning on the animal nervous system.

[0084] Reduce animal mortality:

[0085] Optimize air flow design: By installing a spoiler fan 3 and a spoiler fan 2 4, and arranging an air inlet valve 5 and an exhaust valve 6 between and opposite them, uniform gas flow in the box is ensured, effectively avoiding animal death caused by uneven gas concentration.

[0086] Precise control of gas concentration: The solenoid valves installed on the inlet valve 5 and the exhaust valve 6 allow the PLC programmable logic controller to perform precise control, thereby maintaining a stable carbon monoxide concentration in the box and reducing animal deaths caused by concentration fluctuations.

[0087] Spare gas valve and threaded port: The design of the spare gas valve 7 and the spare threaded port 8 makes it possible to adjust the gas concentration or connect a gas concentration monitor in an emergency, further reducing the risk of animal death due to abnormal gas concentration.

[0088] Animal ethics:

[0089] Reduce animal suffering: By optimizing the airflow design and precisely controlling the gas concentration, we can ensure that animals experience minimal pain and discomfort during the experiment.

[0090] Easy to monitor and adjust: After the spare threaded port 8 is connected to the gas concentration monitor, the gas concentration in the box can be monitored in real time to ensure that the experimental conditions meet the requirements of animal ethics.

[0091] Easy to operate and maintain: The sealing ring and clip design on the edge of the upper cover 2 make the container easy to seal and open, which is convenient for experimenters to operate and maintain, and also reduces the harm to animals caused by improper operation.

[0092] Reduce mold making costs:

[0093] Efficient use of space: The internal dimensions of the box 1 can stack two mouse cages, which improves space utilization and reduces the number of containers required for the experiment.

[0094] Simplify the operation process: The automatic control of the solenoid valve and the one-way valve reduces the complexity and time cost of manual operation and improves the experimental efficiency.

[0095] Durable Materials and Design: Fixed-jointed panels and optimized airflow design make the container more durable, reducing the increased costs caused by equipment damage or repairs.

[0096] In summary, this high-precision, multifunctional carbon monoxide delayed encephalopathy animal model preparation container achieves precise control of experimental conditions and high-quality preparation of animal models through precise gas control and mixing, automated and intelligent control, safety and reliability assurance, and standardized experimental process and animal model preparation process. This provides strong support for scientific research and helps to further explore the pathophysiological mechanism and treatment methods of carbon monoxide delayed encephalopathy.

[0097] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features, and in the description of the present invention, "plurality" means two or more, unless otherwise clearly and specifically defined.

[0098] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0099] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0100] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0101] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and intent of the present invention.

Claims

1. A container for preparing an animal model of delayed encephalopathy caused by carbon monoxide, comprising a box (1), characterized in that: The interior of the box (1) is a hollow space, the four sides and the bottom of the box (1) are fixedly connected plates, the top of the box (1) is provided with an upper cover (2), a turbulence fan (3) is installed on one side of the box (1), a turbulence fan 2 (4) is installed on the side of the box (1) away from the turbulence fan (3), an air intake valve (5) is provided on the plate between the turbulence fan (3) and the turbulence fan 2 (4), an exhaust valve (6) is provided on the plate on the side of the box (1) away from the air intake valve (5), a spare air valve (7) is provided on one side of the turbulence fan (3) and the turbulence fan 2 (4), and a spare threaded opening (8) is provided at the bottom of the exhaust valve.

2. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 1, characterized in that: The box body (1) is a rectangular box body, the spoiler fan (3) is fixedly connected to the box body (1), the spoiler fan (3) and the second spoiler fan (4) are symmetrically distributed along the center line of the box body (1), the spoiler fan (3) is arranged near the top of the box body (1) plate, and the second spoiler fan (4) is arranged near the bottom of the box body (1) plate, and the two fans are staggered.

3. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 1, characterized in that: There are three air intake valves (5) which are evenly spaced along the central axis of the box body (1), and the air intake valves are arranged near the bottom of the box body (1).

4. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 3, characterized in that: There are three exhaust valves (6) which are evenly spaced along the central axis of the box body (1), and the intake valve is arranged near the top of the box body (1).

5. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 1, characterized in that: The centers of the spare gas valves (7) on both sides are collinear and are both arranged on the central axis of the box body (1).

6. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 1, characterized in that: A sealing ring is provided at the edge of the upper cover (2), and clips are provided around the edges for easy locking.

7. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 1, characterized in that: The inner dimensions of the box (1) can accommodate two squirrel cages.

8. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 1, characterized in that: The spare threaded port (8) is a M20*1.5 standard thread and is used for connecting a gas concentration monitor.

9. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 4, characterized in that: Solenoid valves are installed on both the air inlet valve (5) and the air outlet valve (6) to facilitate PLC control.

10. The container for preparing an animal model of delayed encephalopathy caused by carbon monoxide according to claim 5, characterized in that: The spare air valves (7) are respectively equipped with one-way valves to prevent the air flow from flowing back.

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