Enzyme catalytic reaction device with adaptive temperature regulation function

Abstract

The present invention relates to the technical field of enzyme catalytic reaction devices, and in particular to an enzyme catalytic reaction device with an adaptive temperature regulation function, including a reactor tank body, a heating jacket, which is sleeved on the outside of the reactor tank body and forms a heating cavity with the reactor tank body, an oil circuit circulation component, and the oil circuit circulation component includes a circulation pipeline, a circulation pump and a heater, and a temperature control unit for controlling the heating temperature of the heater. The present invention obtains the temperature control curve of i enzyme catalytic reactions at time T, and then based on the energy consumption values ​​of different temperature control curves, presets an energy consumption threshold, retains the temperature control curve with an energy consumption value less than or equal to the energy consumption threshold, and then controls the heater based on the temperature control curve. Such a temperature control method overcomes the defects of the prior art that a fixed temperature is used for control, that is, reduces the energy consumption required for reaction heating, improves the quality of the product and the conversion rate of the enzyme catalytic reaction.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme catalysis reaction devices, in particular to an enzyme catalysis reaction device with a self-adaptive temperature regulation function. Background Art

[0002] Enzymes are highly specific and efficient biocatalysts. Most enzymes are proteins produced by living cells. The catalytic conditions of enzymes are mild and can be carried out at room temperature and pressure. Enzyme-catalyzed reactions are called enzymatic reactions, which are faster than corresponding non-catalytic reactions. In the current biotechnology field, enzyme-catalyzed reactions are the core process of biological transformation. Their efficiency and product quality are highly dependent on the precise control of reaction conditions, especially temperature. Traditional enzyme-catalyzed reaction devices mostly use fixed temperature settings, that is, the temperature setting for the reaction is directly carried out at the optimal temperature to ensure enzyme activity.

[0003] A Chinese patent application with publication number CN117448154A discloses an internal circulation catalytic reactor for bio-enzymes. The invention includes a shell, a heating plate, a detection component and a display controller. The inner wall of the shell is evenly equipped with heating plates for controlling the temperature. The detection component is installed on the inner wall of the shell to detect enzyme reaction data; it also includes an internal circulation mechanism, a catalytic reaction component and an air scrubbing component. The catalytic reaction device is used to perform a catalytic reaction on the feed liquid, and the feed liquid is re-transported to the catalytic reaction device through the pushing and stirring action of the internal circulation mechanism to realize internal circulation. During the reaction, the air scrubbing component sprays gas to form an impact force to clean the catalytic reaction component. The internal circulation catalytic reactor for bio-enzymes can better ensure the efficient conduct of bio-enzyme catalytic reactions and reduce the cost required for the reaction.

[0004] As mentioned in the above application, the existing enzyme catalytic reaction device generally adopts a fixed temperature setting, and the fixed temperature is the optimal reaction temperature of the enzyme activity. Then, the device is heated by a heating plate, and the temperature is controlled to rise to the set fixed temperature, and then the temperature is maintained until the experiment is completed. The defects of this heating method are that, on the one hand, the energy consumption is high, and secondly, the temperature cannot be dynamically adjusted based on the change in conversion rate, which leads to more side reactions and affects the quality of the product. Summary of the invention

[0005] In order to solve the above problems, the present invention provides an enzyme catalytic reaction device with a self-adaptive temperature regulation function.

[0006] The present invention adopts the following technical scheme, an enzyme catalytic reaction device with adaptive temperature regulation function, including a reactor tank, and also includes:

[0007] A heating jacket is arranged outside the reactor tank body and forms a heating cavity between the heating jacket and the reactor tank body, and reactants in the reactor tank body are indirectly heated by introducing heat transfer oil into the heating cavity;

[0008] An oil circulation assembly, the oil circulation assembly comprising a circulation pipeline, a circulation pump and a heater, the heater being used to heat the heat transfer oil transported in the oil circulation assembly;

[0009] A temperature control unit is arranged on the outer wall of the reactor tank and is used to control the heating temperature of the heater. The temperature control unit comprises:

[0010] The raw material acquisition module obtains the reactant composition ratio and catalyst type of the current enzyme-catalyzed reaction;

[0011] A data acquisition module collects temperature control parameters of i historical enzyme-catalyzed reactions corresponding to the current enzyme-catalyzed reaction, and obtains the temperature control curve of the i enzyme-catalyzed reactions at time T, wherein the temperature control parameters of the historical enzyme-catalyzed reactions are collected when the conversion rate of the catalytic reaction product meets the standard;

[0012] The analysis and processing module comprehensively analyzes the temperature control curves at i times T and retains M temperature control curves, where 1<M<i;

[0013] A temperature control module controls the operation of a heater of the enzyme catalytic reaction device based on any one of the M temperature control curves;

[0014] As a further description of the above technical solution: the temperature control unit also includes a temperature optimization module, which is used to collect the comprehensive reaction parameters of the enzyme catalytic reaction device in real time, input them into the trained enzyme catalytic reaction heating temperature prediction machine learning model, obtain the predicted heating temperature, collect the real-time heating temperature of the heater, compare it with the predicted heating temperature, obtain the temperature decay value, compare and analyze the temperature decay value with the preset safety time decay gradient threshold, generate a temperature adjustment instruction, and adjust the heating temperature of the heater based on the temperature adjustment instruction.

[0015] As a further description of the above technical solution: both ends of the circulation pipeline are conductively connected to the heating cavity, the circulation pump and the heater are connected in series to the circulation pipeline, the bottom of the reactor tank body is equipped with legs, and the top of the reactor tank body is conductively connected to the reactant inlet and the catalyst inlet.

[0016] As a further description of the above technical solution: it also includes a stirring mechanism, which is used to stir and mix the reactants in the reactor tank to improve the contact efficiency of the enzyme;

[0017] The stirring mechanism comprises a motor, a stirring shaft and a stirring rod which are bolted to the top center of the reactor tank body. The stirring shaft is rotatably connected to the inner center of the reactor tank body, and the stirring rod is installed on the outer wall of the stirring shaft.

[0018] A sampling port is provided on the top of the reactor tank body, a rotating frame is fixed on the outer wall of the sampling port, a cover plate is fixed on the rotating frame, the cover plate is used to close the sampling port, an observation window is provided at the center of the cover plate, a locking mechanism is provided on the outer wall of the sampling port, the locking mechanism is used to lock and fix the cover plate and the sampling port to achieve sealing of the sampling port;

[0019] A total of several locking mechanisms are provided, and the several locking mechanisms are distributed in a circular shape with equal intervals around the outer side of the sampling port. The locking mechanism includes a concave seat, and a T-shaped locking frame is rotatably connected inside the concave seat. A strip notch is provided on the outer wall of the cover plate for use with the T-shaped locking frame, and the top end of the T-shaped locking frame is threadedly connected to a limit nut that can move up and down.

[0020] As a further description of the above technical solution: the method of performing comprehensive analysis on the temperature control curves at i times T and retaining M temperature control curves includes:

[0021] Obtain the energy consumption values ​​corresponding to the temperature control curves at i times T in sequence;

[0022] Preset energy consumption threshold, and compare and analyze the energy consumption value corresponding to each temperature control curve obtained with the energy consumption threshold;

[0023] The temperature control curves whose energy consumption values ​​are less than or equal to the energy consumption threshold are retained.

[0024] As a further description of the above technical solution: the method for obtaining the energy consumption value of the temperature control curve at the time T includes:

[0025] Divide the time T into n intervals, denoted as t 1 to n ;

[0026] Get the power in each interval , , , where is the power in the Zth interval, is the temperature change value of the Zth interval, is the time of the Zth interval, K is the heat transfer coefficient;

[0027] The energy consumption E of the temperature control curve under time T is obtained by calculating the power of each interval:

[0028] .

[0029] As a further description of the above technical solution: the training method of the enzyme catalytic reaction heating temperature prediction machine learning model includes:

[0030] Obtaining comprehensive reaction parameters and heating temperatures of historical enzyme-catalyzed reactions corresponding to M temperature control curves;

[0031] The comprehensive reaction parameters include reactant composition ratio, catalyst type, reaction time and reactant conversion rate;

[0032] Converting the comprehensive reaction parameters and heating temperature into a corresponding set of feature vectors;

[0033] Each group of feature vectors is used as the input of the machine learning model, and the machine learning model takes the heating temperature corresponding to each group of comprehensive reaction parameters as the output, and the heating temperature actually corresponding to each group of comprehensive reaction parameters is used as the prediction target. Minimizing the loss function value of the machine learning model is used as the training goal, and training is stopped when the loss function value of the machine learning model is less than or equal to the preset target loss value.

[0034] As a further description of the above technical solution: the method for obtaining the conversion rate of the reactant includes:

[0035] ;

[0036] In the formula, is the conversion rate, is the initial reactant concentration, is the current reactant concentration, wherein the reactant concentration is acquired in real time through an online conductivity sensor.

[0037] As a further description of the above technical solution: the expression of the temperature decay value is:

[0038] ;

[0039] In the formula, is the temperature decay value, is the predicted heating temperature, Real-time heating temperature for the collected heater.

[0040] As a further description of the above technical solution: the temperature adjustment instruction includes a first adjustment instruction, a second adjustment instruction, a third adjustment instruction and a fourth adjustment instruction, the first adjustment instruction and the second adjustment instruction are temperature reduction instructions, and the reduced temperatures decrease in sequence, the third adjustment instruction and the fourth adjustment instruction are temperature increase instructions, and the increased temperatures increase in sequence;

[0041] The method of comparing and analyzing the temperature decay value with the preset safety time decay gradient threshold and generating a temperature adjustment instruction includes:

[0042] The preset safe time decay gradient threshold is SBw 1 SBw 2 SBw 3 and SBw 4 , and SBw 1 <SBw 2 <0<SBw 3 <SBw 4 ;

[0043] when <SBw 1 When , a first adjustment instruction is generated;

[0044] When SBw 1 ≤ <SBw 2 When , a second adjustment instruction is generated;

[0045] When SBw 2 ≤ <SBw 3 When , no temperature adjustment instruction is generated;

[0046] When SBw 3 ≤ ≤SBw 4 When , a third adjustment instruction is generated;

[0047] when >SBw 4 When a fourth adjustment instruction is generated.

[0048] Beneficial effects:

[0049] The enzyme catalytic reaction device with adaptive temperature regulation function provided by the present invention obtains the reactant component ratio and catalyst type of the current enzyme catalytic reaction, collects the temperature control parameters of i historical enzyme catalytic reactions corresponding to the current enzyme catalytic reaction, obtains the temperature control curves of the i enzyme catalytic reactions at time T, and then presets the energy consumption threshold based on the energy consumption values ​​of different temperature control curves, retains the temperature control curve with an energy consumption value less than or equal to the energy consumption threshold, and then controls the heater based on the temperature control curve. Such a temperature control method overcomes the defect of the prior art that a fixed temperature is used for control, that is, the energy consumption required for reaction heating is reduced, and refined temperature control is achieved, the occurrence of side reactions is reduced, and the quality of the product and the conversion rate of the enzyme catalytic reaction are improved;

[0050] Furthermore, the comprehensive reaction parameters and heating temperatures of the historical enzyme-catalyzed reactions corresponding to M temperature control curves are obtained, and a machine learning model for predicting the heating temperature of the enzyme-catalyzed reaction is trained based on the obtained comprehensive reaction parameters and heating temperatures. Then, the comprehensive reaction parameters of the enzyme-catalyzed reaction device are collected in real time and input into the trained machine learning model for predicting the heating temperature of the enzyme-catalyzed reaction to obtain the predicted heating temperature. The real-time temperature is collected and compared with the predicted temperature for analysis, and the heating temperature is further optimized. This allows the heater of the enzyme-catalyzed reaction device to be controlled based on a predetermined temperature control curve. The heating temperature of the heater can be dynamically adjusted in real time based on the reaction time and the conversion rate of the reactants, thereby automatically optimizing the temperature control curve according to the reaction stage, realizing intelligent and refined temperature control, and improving reaction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] The present invention will be further explained below in conjunction with the accompanying drawings and embodiments:

[0052] Figure 1 Schematic diagram of the structure of the enzyme catalytic reaction device with adaptive temperature regulation function provided in the embodiment of the present invention Figure 1 ;

[0053] Figure 2 Schematic diagram of the structure of the enzyme catalytic reaction device with adaptive temperature regulation function provided in the embodiment of the present invention Figure 2 ;

[0054] Figure 3 The embodiment of the present invention provides Figure 1 A magnified view of area A in;

[0055] Figure 4 A module connection diagram of a temperature control unit provided in an embodiment of the present invention;

[0056] Figure 5 This is an electrical connection diagram of the temperature control unit and the heater provided in an embodiment of the present invention.

[0057] Figure numerals: 1. Reactor tank; 11. Support legs; 13. Catalyst inlet; 14. Reactant inlet; 2. Heating jacket; 21. Heating cavity; 3. Oil circulation assembly; 31. Circulation pipeline; 32. Circulation pump; 33. Heater; 4. Temperature control unit; 5. Sampling port; 51. Rotating frame; 52. Cover plate; 53. Observation window; 54. Locking mechanism; 541. Concave seat; 542. T-type locking frame; 543. Strip notch; 544. Limit nut; 6. Stirring mechanism; 61. Motor; 62. Stirring shaft; 63. Stirring rod. DETAILED DESCRIPTION

[0058] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below with reference to specific diagrams. It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict.

[0059] Example 1

[0060] See also Figure 1-Figure 5 The embodiment of the present invention provides a technical solution: an enzyme catalytic reaction device with an adaptive temperature regulation function, comprising a reactor tank 1, a support leg 11 is installed at the bottom of the reactor tank 1, and a reactant inlet 14 and a catalyst inlet 13 are conductively connected to the top of the reactor tank 1, and further comprising:

[0061] A heating jacket 2 is arranged outside the reactor tank 1 and forms a heating cavity 21 with the reactor tank 1. The reactants in the reactor tank 1 are indirectly heated by passing heat transfer oil into the heating cavity 21.

[0062] The oil circulation assembly 3 includes a circulation pipeline 31, a circulation pump 32 and a heater 33. The heater 33 is used to heat the heat transfer oil transported in the oil circulation assembly 3. Both ends of the circulation pipeline 31 are connected to the heating cavity 21. The circulation pump 32 and the heater 33 are connected in series to the circulation pipeline 31.

[0063] The temperature control unit 4 is arranged on the outer wall of the reactor tank 1 and is used to control the heating temperature of the heater 33. The temperature control unit 4 includes:

[0064] Raw material acquisition module: obtain the reactant composition ratio and catalyst type of the current enzyme-catalyzed reaction;

[0065] It should be noted that the reactant composition ratio and catalyst type of the current enzyme-catalyzed reaction can be directly obtained from production data or a production schedule, and can be directly input into the temperature control unit 4 .

[0066] Data acquisition module; collecting temperature control parameters of i historical enzyme-catalyzed reactions corresponding to the current enzyme-catalyzed reaction, and obtaining the temperature control curve of the i enzyme-catalyzed reactions at time T, wherein the temperature control parameters of the historical enzyme-catalyzed reactions are collected when the conversion rate of the catalytic reaction product meets the standard;

[0067] Among them, the temperature control parameters of i historical enzyme-catalyzed reactions corresponding to the current enzyme-catalyzed reaction are collected, and the reactant composition ratio and catalyst type of the above historical enzyme-catalyzed reactions are the same as the reactant composition ratio and catalyst type of the current enzyme-catalyzed reaction.

[0068] It should be noted that the temperature control curve of i enzyme-catalyzed reactions at time T can be directly retrieved from the control data recording the historical work of the enzyme-catalyzed reaction device, and then directly entered.

[0069] It should be noted that in enzyme-catalyzed reactions, the temperature is usually increased at a certain rate in the early stages of the reaction to promote an increase in the reaction rate and help quickly reach the optimal reaction state. When the conversion rate reaches its peak, the heating temperature is moderately lowered to reduce the risk of side reactions or catalyst deactivation and ensure stable operation of the system. When the reaction is nearing the end, the temperature is moderately lowered to reduce energy consumption and possible side reactions to ensure safety.

[0070] The analysis and processing module comprehensively analyzes the temperature control curves at i times T and retains M temperature control curves, where 1<M<i;

[0071] A method of comprehensively analyzing the temperature control curves at i times T and retaining M temperature control curves includes:

[0072] Obtain the energy consumption values ​​corresponding to the temperature control curves at i times T in sequence;

[0073] Preset energy consumption threshold, and compare and analyze the energy consumption value corresponding to each temperature control curve obtained with the energy consumption threshold;

[0074] The temperature control curves whose energy consumption values ​​are less than or equal to the energy consumption threshold are retained.

[0075] The method for obtaining the energy consumption value of the temperature control curve under time T includes:

[0076] Divide the time T into n intervals, denoted as t 1 to n ;

[0077] Get the power in each interval , , , where is the power in the Zth interval, is the temperature change value of the Zth interval, is the time of the Zth interval, K is the heat transfer coefficient;

[0078] The energy consumption E of the temperature control curve under time T is obtained by calculating the power of each interval:

[0079] .

[0080] The temperature control module controls the operation of the heater 33 of the enzyme catalytic reaction device based on any one of the M temperature control curves.

[0081] In this embodiment, by obtaining the reactant composition ratio and catalyst type of the current enzyme-catalyzed reaction, collecting the temperature control parameters of i historical enzyme-catalyzed reactions corresponding to the current enzyme-catalyzed reaction, obtaining the temperature control curves of the i enzyme-catalyzed reactions at time T, and then based on the energy consumption values ​​of different temperature control curves, preset energy consumption thresholds, retaining the temperature control curves with energy consumption values ​​less than or equal to the energy consumption threshold, and then controlling the heater 33 based on the temperature control curve. This temperature control method overcomes the defects of the prior art of using a fixed temperature for control, that is, reducing the energy consumption required for reaction heating, and realizing refined temperature control, reducing the occurrence of side reactions, and improving the quality of the product and the conversion rate of the enzyme-catalyzed reaction.

[0082] Example 2

[0083] Reference Figure 1-Figure 5 , a temperature optimization module, which collects the comprehensive reaction parameters of the enzyme catalytic reaction device in real time, inputs them into the trained enzyme catalytic reaction heating temperature prediction machine learning model, obtains the predicted heating temperature, collects the real-time heating temperature of the heater 33, compares it with the predicted heating temperature, obtains the temperature decay value, compares and analyzes the temperature decay value with the preset safety time decay gradient threshold, generates a temperature adjustment instruction, and adjusts the heating temperature of the heater 33 based on the temperature adjustment instruction.

[0084] The training method of the machine learning model for predicting the heating temperature of enzyme-catalyzed reactions includes:

[0085] Obtaining comprehensive reaction parameters and heating temperatures of historical enzyme-catalyzed reactions corresponding to M temperature control curves;

[0086] Comprehensive reaction parameters include reactant composition ratio, catalyst type, reaction time and reactant conversion rate;

[0087] Converting the comprehensive reaction parameters and heating temperature into a corresponding set of feature vectors;

[0088] Each set of feature vectors is used as the input of the machine learning model. The machine learning model takes the heating temperature corresponding to each set of comprehensive reaction parameters as the output, and the heating temperature actually corresponding to each set of comprehensive reaction parameters as the prediction target. Minimizing the loss function value of the machine learning model is used as the training goal. Training is stopped when the loss function value of the machine learning model is less than or equal to the preset target loss value.

[0089] The machine learning model may be one of support vector machine regression, random forest regression or neural network regression models.

[0090] The loss function value of the machine learning model is the mean square error, which is one of the commonly used loss functions. Minimization is used as the goal to train the model so that the machine learning model better fits the data, thereby improving the performance and accuracy of the model;

[0091] In the loss function is the loss function value of the machine learning model, is the feature vector group number; is the number of eigenvector groups; For the The set of feature vectors predicts the heating temperature, For the The heating temperature to which the group eigenvector actually corresponds.

[0092] Other model parameters of the machine learning model, target loss value, optimization algorithm, training set test set validation set ratio, and loss function optimization are all achieved through actual engineering implementation and continuous experimental tuning.

[0093] The method for obtaining the conversion rate of the reactants includes:

[0094] ;

[0095] In the formula, is the conversion rate, is the initial reactant concentration, is the current reactant concentration, where the reactant concentration is acquired in real time through an online conductivity sensor.

[0096] The expression of temperature decay value is:

[0097] ;

[0098] In the formula, is the temperature decay value, is the predicted heating temperature, The real-time heating temperature of the heater 33 is collected.

[0099] The temperature adjustment instructions include a first adjustment instruction, a second adjustment instruction, a third adjustment instruction and a fourth adjustment instruction, the first adjustment instruction and the second adjustment instruction are temperature reduction instructions, and the reduced temperatures decrease in sequence, the third adjustment instruction and the fourth adjustment instruction are temperature increase instructions, and the increased temperatures increase in sequence;

[0100] The method of comparing and analyzing the temperature decay value with the preset safety time decay gradient threshold and generating a temperature adjustment instruction includes:

[0101] The preset safe time decay gradient threshold is SBw 1 SBw 2 SBw 3 and SBw 4 , and SBw1 <SBw 2 <0<SBw 3 <SBw 4 ;

[0102] when <SBw 1 When , a first adjustment instruction is generated;

[0103] When SBw 1 ≤ <SBw 2 When , a second adjustment instruction is generated;

[0104] When SBw 2 ≤ <SBw 3 When , no temperature adjustment instruction is generated;

[0105] When SBw 3 ≤ ≤SBw 4 When , a third adjustment instruction is generated;

[0106] when >SBw 4 When a fourth adjustment instruction is generated.

[0107] In this embodiment, on the basis of the above embodiment, the comprehensive reaction parameters and heating temperatures of the historical enzyme-catalyzed reactions corresponding to M temperature control curves are further obtained, and an enzyme-catalyzed reaction heating temperature prediction machine learning model is trained based on the obtained comprehensive reaction parameters and heating temperatures. Then, the comprehensive reaction parameters of the enzyme-catalyzed reaction device are collected in real time and input into the trained enzyme-catalyzed reaction heating temperature prediction machine learning model to obtain the predicted heating temperature. Then, the real-time temperature is collected and compared with the predicted temperature for analysis, and then the heating temperature is further optimized. Thus, when the heater 33 of the enzyme-catalyzed reaction device is controlled based on a predetermined temperature control curve, the heating temperature of the heater 33 can be dynamically adjusted in real time based on the reaction time and the conversion rate of the reactants, thereby automatically optimizing the temperature control curve according to the reaction stage, realizing intelligent and refined temperature control, and improving reaction efficiency.

[0108] Example 3

[0109] See also Figure 1-Figure 3 , based on the above embodiment, this embodiment further adds a stirring mechanism 6;

[0110] The stirring mechanism 6 is used to stir and mix the reactants in the reactor tank 1 to improve the contact efficiency of the enzyme;

[0111] The stirring mechanism 6 includes a motor 61 bolted to the top center of the reactor tank 1 , a stirring shaft 62 and a stirring rod 63 . The stirring shaft 62 is rotatably connected to the inner center of the reactor tank 1 , and the stirring rod 63 is installed on the outer wall of the stirring shaft 62 .

[0112] In this embodiment, when the stirring mechanism 6 is in use, the motor 61 is controlled to work, which can drive the stirring shaft 62 to rotate, and the stirring shaft 62 drives the stirring rod 63 to move, so as to stir and mix the reactants in the reactor tank 1;

[0113] By setting the stirring mechanism 6, the reactants are ensured to be evenly mixed, the contact efficiency of the enzyme is improved, the reaction is promoted, and the substrate precipitation is prevented. Secondly, by continuously stirring the reactants, the uniformity and stability of the heat of the reactants are improved.

[0114] A sampling port 5 is provided on the top of the reactor tank 1, a rotating frame 51 is fixed on the outer wall of the sampling port 5, a cover plate 52 is fixed on the rotating frame 51, the cover plate 52 is used to close the sampling port 5, an observation window 53 is provided at the center of the cover plate 52, and a locking mechanism 54 is provided on the outer wall of the sampling port 5, the locking mechanism 54 is used to lock and fix the cover plate 52 and the sampling port 5 to achieve sealing of the sampling port 5;

[0115] A total of several locking mechanisms 54 are provided, and the several locking mechanisms 54 are distributed in a circular shape with equal intervals around the outer side of the sampling port 5. The locking mechanism 54 includes a concave seat 541, and a T-shaped locking frame 542 is rotatably connected inside the concave seat 541. A strip notch 543 used to cooperate with the T-shaped locking frame 542 is opened on the outer wall of the cover plate 52, and the top of the T-shaped locking frame 542 is threadedly connected to a limit nut 544 that can move up and down.

[0116] In this embodiment, the sampling port 5 is provided for sampling and observation during the enzyme catalytic reaction, and a cover plate 52 is provided to seal the sampling port 5. The cover plate 52 is provided with an observation window 53, which can be used to directly observe the reaction conditions inside the reactor tank 1.

[0117] When in use, the cover plate 52 and the sampling port 5 are locked and fixed by the locking mechanism 54. First, the cover plate 52 is flipped over by the rotating frame 51, and the cover plate 52 is flipped over and engaged with the upper opening of the sampling port 5. Then, the T-shaped locking frame 542 is rotated so that the vertical end of the T-shaped locking frame 542 is rotated into the strip-shaped notch 543. Then, the limiting nut 544 is rotated to move up and down on the vertical end of the T-shaped locking frame 542, and the limiting nut 544 on the T-shaped locking frame 542 is tightened. The vertical end of the T-shaped locking frame 542 is provided with an external thread for matching the limiting nut 544.

[0118] The basic principles, main features and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and the description in the specification are only to illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An enzyme catalytic reaction device with adaptive temperature regulation function, comprising a reactor tank (1), characterized in that: Also includes: A heating jacket (2) is arranged on the outside of the reactor tank (1) and forms a heating cavity (21) between the heating jacket and the reactor tank (1). The reactants in the reactor tank (1) are indirectly heated by passing heat transfer oil into the heating cavity (21); An oil circulation assembly (3), the oil circulation assembly (3) comprising a circulation pipeline (31), a circulation pump (32) and a heater (33), the heater (33) being used to heat the heat transfer oil transported in the oil circulation assembly (3); A temperature control unit (4) is arranged on the outer wall of the reactor tank (1) and is used to control the heating temperature of the heater (33). The temperature control unit (4) comprises: The raw material acquisition module obtains the reactant composition ratio and catalyst type of the current enzyme-catalyzed reaction; A data acquisition module collects temperature control parameters of i historical enzyme-catalyzed reactions corresponding to the current enzyme-catalyzed reaction, and obtains the temperature control curve of the i enzyme-catalyzed reactions at time T, wherein the temperature control parameters of the historical enzyme-catalyzed reactions are collected when the conversion rate of the catalytic reaction product meets the standard; The analysis and processing module comprehensively analyzes the temperature control curves at i times T and retains M temperature control curves, where 1<M<i; A temperature control module controls the operation of a heater (33) of the enzyme catalytic reaction device based on any one of the M temperature control curves; The temperature control unit (4) also includes a temperature optimization module; The temperature optimization module is used to collect the comprehensive reaction parameters of the enzyme catalytic reaction device in real time, input them into the trained enzyme catalytic reaction heating temperature prediction machine learning model, obtain the predicted heating temperature, collect the real-time heating temperature of the heater (33), compare it with the predicted heating temperature, obtain the temperature decay value, compare and analyze the temperature decay value with the preset safety time decay gradient threshold, generate a temperature adjustment instruction, and adjust the heating temperature of the heater (33) based on the temperature adjustment instruction; The method of performing comprehensive analysis on the temperature control curves at i times T and retaining M temperature control curves comprises: Obtain the energy consumption values ​​corresponding to the temperature control curves at i times T in sequence; Preset energy consumption threshold, and compare and analyze the energy consumption value corresponding to each temperature control curve obtained with the energy consumption threshold; Keep the temperature control curve whose energy consumption value is less than or equal to the energy consumption threshold; The method for obtaining the energy consumption value of the temperature control curve at the time T includes: Divide the time T into n intervals, recorded as t1 to t n ; Get the power in each interval , , , where is the power in the Zth interval, is the temperature change value of the Zth interval, is the time of the Zth interval, K is the heat transfer coefficient; The energy consumption E of the temperature control curve under time T is obtained by calculating the power of each interval: 。 2. The enzyme catalytic reaction device with adaptive temperature regulation function according to claim 1, characterized in that: Both ends of the circulation pipeline (31) are conductively connected to the heating cavity (21); the circulation pump (32) and the heater (33) are connected in series to the circulation pipeline (31); a support leg (11) is installed at the bottom of the reactor tank (1); and a reactant inlet (14) and a catalyst inlet (13) are conductively connected to the top of the reactor tank (1).

3. The enzyme catalytic reaction device with adaptive temperature regulation function according to claim 1, characterized in that: It also includes a stirring mechanism (6), wherein the stirring mechanism (6) is used to stir and mix the reactants in the reactor tank (1); The stirring mechanism (6) comprises a motor (61) bolted to the top center of the reactor tank (1), a stirring shaft (62) and a stirring rod (63), wherein the stirring shaft (62) is rotatably connected to the inner center of the reactor tank (1), and the stirring rod (63) is mounted on the outer wall of the stirring shaft (62); A sampling port (5) is provided on the top of the reactor tank (1); a rotating frame (51) is fixed on the outer wall of the sampling port (5); a cover plate (52) is fixed on the rotating frame (51); the cover plate (52) is used to seal the sampling port (5); an observation window (53) is provided at the center of the cover plate (52); a locking mechanism (54) is provided on the outer wall of the sampling port (5); the locking mechanism (54) is used to lock and fix the cover plate (52) and the sampling port (5) to achieve sealing of the sampling port (5); A plurality of locking mechanisms (54) are provided, and the plurality of locking mechanisms (54) are distributed in a circular shape at equal intervals around the outer side of the sampling port (5), the locking mechanism (54) comprising a concave seat (541), a T-shaped locking frame (542) being rotatably connected inside the concave seat (541), a strip-shaped notch (543) for use with the T-shaped locking frame (542) being provided on the outer wall of the cover plate (52), and a limit nut (544) capable of moving up and down being threadedly connected to the top end of the T-shaped locking frame (542).

4. The enzyme catalytic reaction device with adaptive temperature regulation function according to claim 1, characterized in that: The training method of the enzyme catalyzed reaction heating temperature prediction machine learning model includes: Obtaining comprehensive reaction parameters and heating temperatures of historical enzyme-catalyzed reactions corresponding to M temperature control curves; The comprehensive reaction parameters include reactant composition ratio, catalyst type, reaction time and reactant conversion rate; Converting the comprehensive reaction parameters and heating temperature into a corresponding set of feature vectors; Each group of feature vectors is used as the input of the machine learning model, and the machine learning model takes the heating temperature corresponding to each group of comprehensive reaction parameters as the output, and the heating temperature actually corresponding to each group of comprehensive reaction parameters is used as the prediction target. Minimizing the loss function value of the machine learning model is used as the training goal, and training is stopped when the loss function value of the machine learning model is less than or equal to the preset target loss value.

5. The enzyme catalytic reaction device with adaptive temperature regulation function according to claim 4, characterized in that: The method for obtaining the conversion rate of the reactant comprises: ； In the formula, is the conversion rate, is the initial reactant concentration, is the current reactant concentration.

6. The enzyme catalytic reaction device with adaptive temperature regulation function according to claim 1, characterized in that: The expression of the temperature decay value is: ； In the formula, is the temperature decay value, is the predicted heating temperature, The real-time heating temperature of the heater (33) is collected.

7. The enzyme catalytic reaction device with adaptive temperature regulation function according to claim 6, characterized in that: The temperature adjustment instruction includes a first adjustment instruction, a second adjustment instruction, a third adjustment instruction and a fourth adjustment instruction, wherein the first adjustment instruction and the second adjustment instruction are temperature reduction instructions, and the reduced temperatures decrease in sequence, and the third adjustment instruction and the fourth adjustment instruction are temperature increase instructions, and the increased temperatures increase in sequence; The method of comparing and analyzing the temperature decay value with the preset safety time decay gradient threshold and generating a temperature adjustment instruction includes: The preset safety time decay gradient thresholds are SBw1, SBw2, SBw3 and SBw4, and SBw1<SBw2<0<SBw3<SBw4; when When <SBw1, the first adjustment instruction is generated; When SBw1≤ When <SBw2, a second adjustment instruction is generated; When SBw2≤ When <SBw3, no temperature adjustment instruction is generated; When SBw3≤ When ≤SBw4, the third adjustment instruction is generated; when >SBw4, a fourth adjustment instruction is generated.

Images