Improved biochemical incubator

Abstract

The invention belongs to the technical field, and particularly relates to an improved biochemical incubator which comprises a suspension culture assembly, a position adjusting assembly and an ultrasonic adjusting assembly, the suspension culture assembly comprises a spherical culture cavity, the spherical culture cavity is a transparent culture cavity, and multiple groups of stable acoustic potential wells are arranged in the spherical culture cavity and used for suspension culture; the position adjusting assembly is mounted on the outer side of the spherical culture cavity and is used for adjusting the position of the ultrasonic adjusting assembly; the ultrasonic wave adjusting assembly comprises an ultrasonic wave generator, and a plurality of stable acoustic potential wells are formed in the spherical culture cavity. The ultrasonic wave suspension culture device has the beneficial effects that the plurality of stable acoustic potential wells are formed by utilizing ultrasonic waves, the suspension culture of cell tissues is realized, and contact pollution in a traditional culture method is avoided; the four groups of annular mounting frames are perpendicular to the equator plane of the spherical culture cavity, the position of the ultrasonic generator can be flexibly adjusted, and the stability of the acoustic potential well is ensured.

Description

Technical Field

[0001] The invention relates to the technical field, and in particular to an improved biochemical incubator. Background Art

[0002] Acoustic Levitation Cultivation is a technology that uses the physical properties of sound waves to suspend and culture cells or tissues without contact. This technology is mainly based on the sound pressure effect generated when sound waves propagate in the medium. It can allow cells or tissues to suspend freely in three-dimensional space and grow and differentiate without using traditional supports.

[0003] In the related technology, the existing acoustic levitation culture technology forms a stable acoustic potential well (node) in the suspension area through an ultrasonic generator. The suspended sample remains stable in this area and is suitable for the suspension culture of single cells or small-scale tissues. However, the number of suspension points in a single acoustic well is limited, and it is difficult to process multiple samples at the same time. It is more difficult to culture tissue structures that require the construction of complex geometric shapes. At the same time, it is more difficult to adjust the ultrasonic generator and cannot be widely used in biological agriculture. For this reason, we propose an improved biochemical incubator.

[0004] The above information disclosed in this Background section is only for understanding the background of the present inventive concept and therefore it may contain information that does not constitute prior art. Summary of the invention

[0005] In order to overcome the shortcomings of the prior art, the present invention provides an improved biochemical incubator to solve the problems proposed in the above-mentioned background technology, that is, the number of suspension points of a single acoustic trap is limited, it is difficult to process multiple samples at the same time, it is difficult to culture tissue structures that require the construction of complex geometric shapes, and it is difficult to adjust the ultrasonic generator, and it cannot be widely used in biological agriculture.

[0006] The technical solution adopted by the present invention to solve the technical problem is: an improved biochemical incubator, including a suspension culture component, a position adjustment component and an ultrasonic adjustment component.

[0007] The suspension culture component comprises a spherical culture chamber, which is a transparent culture chamber, a material taking port is provided at the upper end of the spherical culture chamber, a material discharge port is provided at the lower end side of the spherical culture chamber, and a cover plate is detachably sealed on the material taking port and the material discharge port, and a plurality of stable acoustic potential wells are provided in the spherical culture chamber for suspending the culture;

[0008] The position adjustment component is installed on the outside of the spherical culture chamber and is used to adjust the position of the ultrasonic adjustment component. The position adjustment component includes an annular mounting frame, and the annular mounting frame is provided with four groups. The annular mounting frame is perpendicular to the equatorial plane of the spherical culture chamber, and the ultrasonic adjustment component is movably installed in the annular mounting frame;

[0009] The ultrasonic adjustment component includes an ultrasonic generator for generating ultrasonic waves to form multiple stable acoustic potential wells in the spherical culture cavity, and to suspend and culture cell tissues at the acoustic potential wells. The ultrasonic generator is arranged on the inner side of the annular mounting frame, and the emission direction of the ultrasonic generator points to the spherical culture cavity.

[0010] As an optimized technical solution, the suspension culture component also includes a fixing ring, which is symmetrically arranged in two groups. The fixing rings use the equatorial plane of the spherical culture cavity as the symmetry axis and are symmetrically fixed on the side of the spherical culture cavity. A rotation groove is opened on the side of the fixing rings that are close to each other. A rotating arc plate is rotatably arranged in the rotation groove. The inner side of the rotating arc plate is fitted on the side of the spherical culture cavity. A clamping plate is symmetrically and vertically fixed at the upper and lower ends of the rotating arc plate. A ball is installed inside the clamping plate. The clamping plate is clamped in the rotation groove of the fixing ring. The clamping plate and the rotating arc plate rotate in the fixing ring through the ball. A connecting plate is integrally formed at the central position of the side of the rotating arc plate away from the spherical culture cavity.

[0011] As an optimized technical solution, the ultrasonic adjustment component also includes a rotating mounting plate, the upper end of the ultrasonic generator is connected to the rotating mounting plate through a connecting rod, the side of the rotating mounting plate is connected to a direction adjustment motor through a connecting shaft, the direction adjustment motor is fixed to the lower end of the connecting seat through a rotating seat, a transmission shaft is penetrated through the side of one end of the connecting seat, the transmission shaft rotates in the inner wall of the connecting seat, one end of the transmission shaft is connected to the output end of the driving motor, a driving gear is fixedly installed on the side of the transmission shaft, the driving gear is meshed with the inner gear ring inside the annular mounting frame, the other side of the connecting seat is connected to the output end of the adjustment motor through a connecting shaft, and the connecting seat and the connecting shaft are arranged to follow.

[0012] As an optimized technical solution, the annular mounting frame is fixedly connected at the upper and lower end poles of the spherical culture chamber, two groups of side through grooves are symmetrically opened on the side of the annular mounting frame, an inner through groove is opened on the inner side of the annular mounting frame, and an inner gear ring is integrally formed on one side of the inner through groove of the annular mounting frame, a transmission shaft is passed through one of the side through grooves, and the end of the transmission shaft away from the annular mounting frame is connected to the output end of the driving motor, and a connecting shaft is passed through the inside of the other side through groove, and the end of the connecting shaft away from the annular mounting frame is connected to the output end of the adjustment motor.

[0013] As an optimized technical solution, the incubator also includes a rotating component for adjusting the positions of the annular mounting frame and the ultrasonic generator, the rotating component includes an upper fixed plate, a rotating motor is fixedly installed at the lower center position of the upper fixed plate, the lower output end of the rotating motor is connected to the upper end pole of the annular mounting frame through a rotating shaft, a plurality of groups of rotating plates are fixedly arranged on the side of the rotating shaft, a connecting rod is vertically fixed at the lower end of the rotating plate away from the rotating shaft, the lower end of the connecting rod is connected and fixed to the upper end of the annular mounting frame and the upper end of the connecting plate, the lower end of the lower end pole of the annular mounting frame is vertically rotatably arranged in a rotating sleeve through a rotating shaft, and the rotating sleeve is fixedly installed at the upper center position of the lower fixed plate.

[0014] As an optimized technical solution, the incubator also includes a sound-absorbing isolation component for reducing the influence of external vibration or noise on the spherical incubation cavity and maintaining the stability of the sound field. The sound-absorbing isolation component is arranged on the outside of the rotating component. The sound-absorbing isolation component includes an isolation box, the inner wall of the isolation box is connected and fixed to the upper fixed plate and the lower fixed plate, the inner wall of the isolation box is made of sound insulation material, a mounting sleeve is fitted on the upper end of the side of the isolation box, and multiple groups of side shock-absorbing pads are symmetrically installed on the side of the mounting sleeve. The side of the side shock-absorbing pad away from the mounting sleeve is fitted with the inner wall of the box body, and a support plate is provided at the lower end of the mounting sleeve. The support plate is fixedly arranged on the side of the isolation box, and handles are fixedly installed on the left and right sides of the lower end of the support plate. Multiple groups of lower end buffer columns are vertically mounted on the lower end of the isolation box, and the lower ends of the lower end buffer columns are connected and fixed to the mounting plate, and the side of the mounting plate is connected and fixed to the inner wall of the box body. The lower end buffer column includes a buffer sleeve and a buffer spring, and multiple groups of high-definition cameras are installed on the side wall and bottom wall of the isolation box.

[0015] As an optimized technical solution, a box door is installed on the upper front end of the box body, a pull rod is installed on the right side of the box door, ventilation holes are symmetrically opened on the upper ends of the left and right sides of the box body, a controller is installed on the front end of the box door, and an operating status indicator light is installed on the lower end of the controller on the box door.

[0016] As an optimized technical solution, a plurality of heat dissipation holes are opened at the front end of the box below the mounting plate, a power interface is installed at the lower end of the right side of the box, a wireless charger is installed at the lower end of the mounting plate inside the box, the wireless charger is an electromagnetic induction wireless charger, and a universal wheel is installed at the lower end of the box.

[0017] The beneficial effects of the present invention are:

[0018] Ultrasound is used to form multiple stable acoustic potential wells to achieve suspended culture of cell tissues and avoid contact contamination in traditional culture methods; four sets of annular mounting frames are perpendicular to the equatorial plane of the spherical culture chamber, and can flexibly adjust the position of the ultrasonic generator to ensure the stability of the acoustic potential well; stable support and flexible angle adjustment are provided to ensure that the spherical culture chamber remains stable during the adjustment process; through precise phase and frequency settings, a stable standing wave field is formed in the spherical culture chamber to ensure that the cell tissues are stably suspended in the acoustic potential well; the angle and position adjustment of the ultrasonic generator is achieved, further improving the stability of the acoustic potential well; the position and angle of the annular mounting frame and the ultrasonic generator can be adjusted to ensure the precise positioning of the acoustic potential well; the influence of external vibration and noise on the spherical culture chamber is reduced to maintain the stability of the sound field; the position of the suspended object is monitored in real time, and the position, angle and frequency of the ultrasonic generator are automatically adjusted to ensure that the suspended object is always in the expected position; real-time adjustment is made through the feedback system to ensure the stability of the suspended object in the acoustic potential well, and it can be quickly restored even under external interference. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of an improved biochemical incubator provided by the present invention;

[0020] Figure 2 It is a schematic structural diagram of a rotating assembly of an improved biochemical incubator provided by the present invention;

[0021] Figure 3 It is a structural schematic diagram of a suspension culture component of an improved biochemical culture box provided by the present invention;

[0022] Figure 4 It is a structural schematic diagram of a fixed ring and a rotating arc plate assembly of an improved biochemical incubator provided by the present invention;

[0023] Figure 5 It is a schematic diagram of the structure of a position adjustment component of an improved biochemical incubator provided by the present invention;

[0024] Figure 6 It is a cross-sectional view of a ring-shaped mounting frame of an improved biochemical incubator provided by the present invention;

[0025] Figure 7 It is a schematic structural diagram of an ultrasonic adjustment component of an improved biochemical incubator provided by the present invention;

[0026] Figure 8 It is a structural schematic diagram of a sound-absorbing and isolating component of an improved biochemical incubator provided by the present invention;

[0027] Fig. 9 The present invention provides an improved biochemical incubator provided by the invention.

[0028] Among them: 1 is a box, 2 is a rotating component, 201 is an upper fixed plate, 202 is a rotating motor, 203 is a rotating plate, 204 is a connecting rod, 205 is a lower fixed plate, 206 is a rotating sleeve, 3 is a suspension culture component, 301 is a spherical culture chamber, 302 is a material extraction port, 303 is a fixed ring, 304 is a connecting plate, 305 is a rotating groove, 306 is a clamping plate, 307 is a ball, 308 is a rotating arc plate, 4 is a position adjustment component, 401 is an annular mounting frame, 402 is a side through groove, 403 is an inner through groove, 404 is an inner gear ring, 5 is an ultrasonic adjustment component, 501 is an ultrasonic Wave generator, 502 is a rotating mounting plate, 503 is a direction adjustment motor, 504 is a connecting seat, 505 is a driving motor, 506 is a driving gear, 507 is a transmission shaft, 508 is an adjustment motor, 6 is a sound-absorbing isolation component, 601 is an isolation box, 602 is a mounting sleeve, 603 is a side shock-absorbing pad, 604 is a support plate, 605 is a handle, 606 is a lower end buffer column, 607 is a mounting plate, 608 is a high-definition camera, 7 is a box door, 8 is a controller, 9 is a pull rod, 10 is an operating status indicator light, 11 is a vent, 12 is a power interface, 13 is a universal wheel, and 14 is a heat dissipation hole. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0030] It should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the inventive product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0031] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] Reference Figure 1 , 2 , 3, 4, 5, 6, 7, 8, 9, an improved biochemical incubator, comprising a suspension culture component 3, a position adjustment component 4 and an ultrasonic adjustment component 5,

[0033] The suspension culture component 3 includes a spherical culture chamber 301, which is a transparent culture chamber. A feed port 302 is provided at the upper end of the spherical culture chamber 301, and a discharge port is provided at the lower end side of the spherical culture chamber 301. The feed port 302 and the discharge port are detachably sealed with a cover plate. A plurality of groups of stable acoustic potential wells are provided in the spherical culture chamber 301 for suspending culture. The culture medium in the spherical culture chamber adopts MS culture medium, and the cultured cell tissue is one of Arabidopsis cells, tobacco cells, and rice cells.

[0034] The position adjustment component 4 is installed on the outside of the spherical culture chamber 301 and is used to adjust the position of the ultrasonic adjustment component 5. The position adjustment component 4 includes an annular mounting frame 401. The annular mounting frame 401 is provided with four groups. The annular mounting frame 401 is perpendicular to the equatorial plane of the spherical culture chamber 301. The ultrasonic adjustment component 5 is movably installed in the annular mounting frame 401. A group of ultrasonic generators 501 are symmetrically arranged at the upper and lower poles of the spherical culture chamber 301. Eight groups of ultrasonic generators 501 are symmetrically arranged at each of the equatorial plane, the 45° north latitude line, and the 45° south latitude line.

[0035] The ultrasonic adjustment component 5 includes an ultrasonic generator 501, which is used to generate ultrasonic waves, form multiple stable acoustic potential wells in the spherical culture chamber 301, and suspend the cell tissue in the acoustic potential wells. The ultrasonic generator 501 is arranged on the inner side of the annular mounting frame 401, and the emission direction of the ultrasonic generator 501 points to the spherical culture chamber 301. Acoustic levitation depends on the standing wave field formed by sound waves at a specific frequency and intensity, in which there are nodes and anti-nodes. The suspended tissue will be maintained at the node because the sound pressure at the node is the lowest and the force is balanced. The formation conditions of the standing wave are determined by factors such as the wavelength and frequency of the sound wave and the distance from the reflecting surface. The position of the suspended object z s Usually located at the nodes of the standing wave field: Among them, λ is the wavelength of the sound wave, n is an integer, representing the nth node. Specifically, the mathematical expression of the standing wave field is:

[0036] P(x,y,z)=P0cos(kx-ωt+φ), where P0 is the sound pressure amplitude, k is the wave number, ω is the angular frequency, and φ is the phase;

[0037] Acoustic potential well: The formation of an acoustic potential well depends on the standing wave field formed by sound waves at a specific frequency and intensity. The mathematical model of an acoustic potential well can be expressed by the potential energy formula:

[0038] Where a is the radius of the suspended particle, ρ0 and ρ are the density of the medium and the particle, respectively, P is the sound pressure, and v is the speed of sound;

[0039] The specific phase value of each ultrasonic generator can be calculated by the following formula:

[0040] Among them, φ i is the phase of the ith ultrasonic generator, d i is the distance from the generator to the target sound trap, and λ is the wavelength of the sound wave.

[0041] As an optimized technical solution, the suspension culture component 3 also includes a fixed ring 303, and the fixed ring 303 is symmetrically arranged in two groups. The fixed ring 303 takes the equatorial plane of the spherical culture chamber 301 as the symmetry axis and is symmetrically fixed on the side of the spherical culture chamber 301. A rotating groove 305 is opened on the side close to each other. A rotating arc plate 308 is rotatably arranged in the rotating groove 305. The inner side of the rotating arc plate 308 is fitted on the side of the spherical culture chamber 301. The upper and lower ends of the rotating arc plate 308 are symmetrically and vertically fixed with a clamping plate 306, and the clamping plate 306 is arranged inside. A ball 307 is installed, and the clamping plate 306 is clamped in the rotating groove 305 of the fixed ring 303. The clamping plate 306 and the rotating arc plate 308 rotate in the fixed ring 303 through the ball 307. The rotating arc plate 308 is integrally formed with a connecting plate 304 at the central position of the side away from the spherical culture chamber 301. The fixed ring 303 cooperates with the rotating arc plate 308 to connect and fix the spherical culture chamber 301. The rotating arc plate 308 and the fixed ring 303 can cooperate with the rotating component 2 to adjust the angle of the annular mounting frame 401 while keeping the spherical culture chamber 301 stable.

[0042] As an optimized technical solution, the ultrasonic adjustment component 5 also includes a rotating mounting plate 502, the upper end of the ultrasonic generator 501 is connected to the rotating mounting plate 502 through a connecting rod, and the side of the rotating mounting plate 502 is connected to a direction adjustment motor 503 through a connecting shaft. The direction adjustment motor 503 is fixedly arranged at the lower end of the connecting seat 504 through a rotating seat, and a transmission shaft 507 is penetrated through the side of one end of the connecting seat 504. The transmission shaft 507 rotates in the inner wall of the connecting seat 504, and one end of the transmission shaft 507 is connected to the output end of the drive motor 505. The side of the transmission shaft 507 is fixed. A driving gear 506 is installed, which is meshed with the inner gear ring 404 inside the annular mounting frame 401. The other side of the connecting seat 504 is connected to the output end of the adjustment motor 508 through a connecting shaft. The connecting seat 504 and the connecting shaft are set to follow. The ultrasonic generator 501 changes the emission angle of the sound wave by adjusting the motor 503. The driving gear 506 is meshed with the inner gear ring 404 inside the annular mounting frame 401, and the position is adjusted on the inner side of the annular mounting frame 401. The driving motor 505 drives the transmission shaft 507 to rotate, which will not affect the state of the connecting seat 504.

[0043] As an optimized technical solution, the annular mounting frame 401 is fixedly connected at the upper and lower ends of the spherical culture chamber 301, and two groups of side through grooves 402 are symmetrically opened on the side of the annular mounting frame 401. An inner through groove 403 is opened on the inner side of the annular mounting frame 401. An inner gear ring 404 is integrally formed on one side of the inner through groove 403 of the annular mounting frame 401. A transmission shaft 507 is penetrated in one of the side through grooves 402. The end of the transmission shaft 507 away from the annular mounting frame 401 is connected to the output end of the driving motor 505, and the other side through groove 402 is penetrated by a connecting shaft, and the connecting shaft is away from the annular One end of the mounting frame 401 is connected to the output end of the adjustment motor 508, and the other side of the connecting seat 504 is connected to a connecting shaft and an adjustment motor 508. The adjustment motor 508 is arranged in the through slot 402 on the other side. A limit block and a limit ball are arranged on the side of the adjustment motor 508 in the side through slot 402 to ensure that the adjustment motor 508 can move in the annular mounting frame 401 while the adjustment motor 508 itself does not rotate. When the adjustment motor 508 is working, it drives the connecting shaft to rotate, thereby adjusting the angle of the connecting seat 504 to ensure that the angle of the ultrasonic generator 501 can form a stable acoustic potential well.

[0044] In this embodiment, the incubator further includes a rotating assembly 2 for adjusting the positions of the annular mounting frame 401 and the ultrasonic generator 501. The rotating assembly 2 includes an upper fixed plate 201. A rotating motor 202 is fixedly installed at the central position of the lower end of the upper fixed plate 201. The lower output end of the rotating motor 202 is connected to the upper end pole of the annular mounting frame 401 through a rotating shaft. A plurality of rotating plates 203 are fixedly arranged on the side of the rotating shaft. A connecting rod 204 is vertically fixedly arranged at the lower end of the rotating plate 203 away from the rotating shaft. The lower end of the connecting rod 204 is connected and fixed to the upper end of the annular mounting frame 401 and the upper end of the connecting plate 304. The annular mounting frame 401 The lower end of the lower end pole is vertically rotated through the rotating shaft and is arranged in the rotating sleeve 206. The rotating sleeve 206 is fixedly installed at the upper center position of the lower fixed plate 205. When the rotating motor 202 rotates, it drives the rotating plate 203 to rotate, and the rotating plate 203 drives the connecting rod 204 to rotate. The lower ends of the connecting rod 204 are respectively connected and fixed to the upper ends of the annular mounting frame 401 and the upper ends of the connecting plate 304, driving the annular mounting frame 401 to rotate, thereby realizing the angle adjustment of the annular mounting frame 401. At the same time, since the connecting plate 304 and the rotating arc plate 308 are integrally formed, the annular mounting frame 401 can be rotated while maintaining the stability of the internal spherical culture chamber 301.

[0045] In this embodiment, the incubator further includes a sound-absorbing isolation component 6, which is used to reduce the influence of external vibration or noise on the spherical culture chamber 301 and maintain the stability of the sound field. The sound-absorbing isolation component 6 is arranged on the outside of the rotating component 2, and the sound-absorbing isolation component 6 includes an isolation box 601. The inner wall of the isolation box 601 is connected and fixed to the upper fixing plate 201 and the lower fixing plate 205. The inner wall of the isolation box 601 is made of sound insulation material. The upper end of the side of the isolation box 601 is fitted with a mounting sleeve 602. The side of the mounting sleeve 602 is symmetrically installed with multiple groups of side shock-absorbing pads 603. The side of the side shock-absorbing pad 603 away from the mounting sleeve 602 is connected to the inner wall of the box body 1. The mounting sleeve 602 is provided with a support plate 604 at the lower end, and the support plate 604 is fixedly arranged on the side of the isolation box 1. The left and right sides of the lower end of the support plate 604 are fixedly installed with handles 605. The lower end of the isolation box 1 is vertically clamped with multiple groups of lower end buffer columns 606. The lower end of the lower end buffer column 606 is connected and fixed to the mounting plate 607. The side of the mounting plate 607 is connected and fixed to the inner wall of the box body 1. The lower end buffer column 606 includes a buffer sleeve and a buffer spring. Multiple groups of high-definition cameras 608 are installed on the side wall and the bottom wall of the isolation box 1. The high-definition cameras 608 are used to monitor the actual position of the suspended matter in real time. α and the expected position z s For comparison, if z α Deviation z s , it means that the sound field or external factors affect the suspension state, and the error after calculation is Δz=z α-z s ,The control system determines the direction and amplitude of adjustment according to the error amount. The adjustment methods include position adjustment, angle adjustment and frequency adjustment;

[0046] Position adjustment: The position of the ultrasonic generator 501 is fine-tuned by driving the motor 505, so that the distance of the sound wave reflection surface changes, thereby changing the position of the standing wave node. This adjustment can be expressed as the movement amount of the reflection surface Δd:

[0047]

[0048] Angle adjustment: If the sound field is disturbed asymmetrically, the platform can evenly distribute the sound wave energy by fine-tuning the angle of the ultrasonic generator 501. The angle adjustment amount θ can be calculated by a simple geometric relationship:

[0049] Where L is the length of the sound wave propagation path;

[0050] Frequency adjustment: In some cases, the wavelength λ can be adjusted by fine-tuning the ultrasonic frequency f, thereby changing the position of the standing wave node:

[0051] Where c is the speed of sound;

[0052] Frequency fine-tuning amount Fine tuning of the frequency will cause a change in the wavelength, thus relocating the suspension to the correct node location;

[0053] After performing the adjustment operation, the sensor continues to monitor the position of the suspended object. If the error Δz has been eliminated, the system will maintain the current state; if there is still a deviation, the system will continue to perform the next round of adjustments until the suspended object returns to the expected position.

[0054] Specifically, a box door 7 is installed on the upper front end of the box body 1, a pull rod 9 is installed on the right side of the box door 7, and vents 11 are symmetrically provided on the upper ends of the left and right sides of the box body 1. A controller 8 is installed on the front end of the box door 7. The controller 8 adjusts the position and angle of the ultrasonic generator 501 according to the data of the high-definition camera 608, and regulates the temperature and humidity conditions in the spherical culture chamber 301 to ensure the normal culture of cell tissues. A working condition indicator light 10 is installed on the lower end of the controller 8 on the box door 7. A plurality of heat dissipation holes 14 are provided at the front end of the box body 1 below the mounting plate 607. A power supply interface 12 is installed on the lower right end of the box body 1. A plurality of sets of universal wheels 13 are symmetrically installed at the lower end. A wireless charger is installed at the lower end of the mounting plate 607 inside the box 1. The wireless charger is an electromagnetic induction wireless charger. A wireless charging frequency different from the ultrasonic frequency is selected. A wireless charging frequency of 100kHz can be used to conduct preliminary tests to evaluate the effect of the wireless charger on the acoustic levitation effect. The spherical culture chamber 301 is correspondingly provided with a temperature control component and a humidity control component commonly used in the field of cell culture technology. Before actual cell culture is carried out, a simulation experiment is required to verify the effect of the phase setting of the acoustic trap and the ultrasonic generator 501. Taking four acoustic traps as an example, the following are the implementation steps:

[0055] 1.1 Initial Setup:

[0056] Starting the ultrasonic generator 501: starting the ultrasonic generator 501 according to a preset phase and frequency to form an initial sound field;

[0057] Sample placement: Lightweight particles (such as polystyrene microspheres) are placed in the suspension area. These particles will serve as markers to verify the formation of acoustic traps in the experiment.

[0058] 1.2 Suspension point position verification:

[0059] Observe the suspension state: use the high-definition camera 608 to observe the suspension state of the particles and confirm whether the particles stay in the expected four acoustic trap positions. If the particles are stably suspended in multiple independent areas, it means that the acoustic trap has been successfully formed;

[0060] 1.3 Phase setting verification:

[0061] Adjust the phase: gradually adjust the phase of the ultrasonic generator 501 in the control system, observe the movement of the particles, confirm the independence and stability of each sound trap by gradually increasing or decreasing the phase difference, and record the changes in the spatial position of the particles under different phase settings.

[0062] 1.4 Interference effect verification:

[0063] Test phase synchronization: Set different phase combinations to observe whether there will be acoustic field interference leading to instability of the acoustic trap. You can deliberately adjust the phase difference to an incorrect value and observe the deviation and instability of the particles to confirm the importance of the correct phase combination.

[0064] 1.5 Dynamic Adjustment Verification:

[0065] Introducing disturbances: artificially introducing slight disturbances (such as slight air flow or vibration) and observing whether the suspension point returns to a stable state under the action of the feedback adjustment system to verify the effect of the dynamic adjustment platform;

[0066] 1.6 Long-term stability verification:

[0067] Long-term suspension test: Keep the system running for a long time, observe the stability of the particles in the acoustic trap, and record whether the suspension point drifts or collapses to verify the long-term stability of the system;

[0068] 2. Data Analysis:

[0069] 2.1 Suspension point position data:

[0070] Position stability: Analyze the position data of the suspension points recorded by the camera and calculate the average position and variance of each suspension point to determine its stability;

[0071] 2.2 Phase influence analysis:

[0072] Phase and position relationship: Draw the relationship curve between phase adjustment and suspension point position, analyze the impact of phase adjustment on suspension point position, and confirm the best phase combination;

[0073] 2.3 Interference effect analysis:

[0074] Interference pattern recognition: By clustering the data of the suspension point positions under different phase combinations, it is possible to identify whether there is an interference pattern. If the interference effect is obvious, the phase setting should be readjusted.

[0075] 2.4 Adjust system performance:

[0076] Feedback response time: measures the time required for the suspension point to return to a stable state after the introduction of a disturbance, in order to evaluate the response speed and efficiency of the dynamic regulation system;

[0077] 3. Experimental results verification:

[0078] 3.1 Formation of four acoustic traps:

[0079] Position stability: The experimental data should show that the particles are stably suspended at the four preset positions (acoustic traps) with small variance, indicating that the formation of the acoustic trap is successful;

[0080] 3.2 Effect of phase setting:

[0081] Phase Adjustment Verification: Data should show that the positional stability of the suspension point is highest when the optimal phase combination is used, and there is no significant interference effect between the acoustic traps.

[0082] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot limit the scope of protection of the present invention in turn. Various combinations, modifications or equivalent substitutions of the technical solutions of the present invention by technicians in this field do not depart from the spirit and scope of the technical solutions of the present invention, and should be included in the scope of the claims of the present invention.

Claims

1. An improved biochemical incubator, comprising a suspension culture component (3), a position adjustment component (4) and an ultrasonic adjustment component (5), characterized in that: The suspension culture component (3) comprises a spherical culture chamber (301), the spherical culture chamber (301) is a transparent culture chamber, a material taking port (302) is provided at the upper end of the spherical culture chamber (301), a material discharge port is provided at the lower end side of the spherical culture chamber (301), and a cover plate is detachably sealed on the material taking port (302) and the material discharge port, and a plurality of groups of stable acoustic potential wells are provided in the spherical culture chamber (301) for suspending culture; The position adjustment component (4) is installed on the outside of the spherical culture chamber (301) and is used to adjust the position of the ultrasonic adjustment component (5). The position adjustment component (4) comprises an annular mounting frame (401). The annular mounting frame (401) is provided with four groups. The annular mounting frame (401) is perpendicular to the equatorial plane of the spherical culture chamber (301). The ultrasonic adjustment component (5) is movably installed in the annular mounting frame (401); The ultrasonic adjustment component (5) comprises an ultrasonic generator (501) for generating ultrasonic waves, forming a plurality of stable acoustic potential wells in the spherical culture chamber (301), and suspending and culturing the cell tissue at the acoustic potential wells. The ultrasonic generator (501) is arranged on the inner side of the annular mounting frame (401), and the emission direction of the ultrasonic generator (501) points to the spherical culture chamber (301).

2. An improved biochemical incubator according to claim 1, characterized in that: The suspension culture component (3) further comprises a fixing ring (303), wherein two groups of the fixing rings (303) are symmetrically arranged, and the fixing rings (303) are symmetrically fixedly arranged on the side of the spherical culture chamber (301) with the equatorial plane of the spherical culture chamber (301) as the symmetry axis, and a rotation groove (305) is provided on the side close to each other, and a rotation arc plate (308) is rotatably arranged in the rotation groove (305), and the inner side of the rotation arc plate (308) is arranged in contact with the side of the spherical culture chamber (301). The upper and lower ends of the rotating arc plate (308) are symmetrically and vertically fixed with a clamping plate (306), and a ball (307) is installed inside the clamping plate (306). The clamping plate (306) is clamped in the rotating groove (305) of the fixed ring (303). The clamping plate (306) and the rotating arc plate (308) rotate in the fixed ring (303) through the ball (307). A connecting plate (304) is integrally formed at the central position of one side of the rotating arc plate (308) away from the spherical culture chamber (301).

3. The improved biochemical incubator according to claim 1, characterized in that: The ultrasonic adjustment component (5) further comprises a rotating mounting plate (502), the upper end of the ultrasonic generator (501) is connected to the rotating mounting plate (502) via a connecting rod, a direction adjustment motor (503) is connected to the side of the rotating mounting plate (502) via a connecting shaft, the direction adjustment motor (503) is fixedly arranged at the lower end of the connecting seat (504) via a rotating seat, a transmission shaft (507) is penetrated through the side of one end of the connecting seat (504), and the transmission shaft (507) is connected to the connecting seat (504) via a connecting shaft. The connecting seat (504) rotates inside the inner wall, one end of the transmission shaft (507) is connected to the output end of the driving motor (505), a driving gear (506) is fixedly installed on the side of the transmission shaft (507), and the driving gear (506) is meshed with the inner gear ring (404) inside the annular mounting frame (401), and the other side of the connecting seat (504) is connected to the output end of the adjustment motor (508) through the connecting shaft, and the connecting seat (504) is arranged to move with the connecting shaft.

4. The improved biochemical incubator according to claim 1, characterized in that: The annular mounting frame (401) is fixedly connected at the upper and lower end poles of the spherical culture chamber (301), and two groups of side through grooves (402) are symmetrically opened on the side of the annular mounting frame (401). An inner through groove (403) is opened on the inner side of the annular mounting frame (401), and an inner gear ring (404) is integrally formed on one side of the inner through groove (403) of the annular mounting frame (401). A transmission shaft (507) is passed through one of the side through grooves (402), and the end of the transmission shaft (507) away from the annular mounting frame (401) is connected to the output end of the driving motor (505), and a connecting shaft is passed through the inside of the other side through groove (402), and the end of the connecting shaft away from the annular mounting frame (401) is connected to the output end of the adjustment motor (508).

5. The improved biochemical incubator according to claim 1, characterized in that: The incubator further comprises a rotating assembly (2) for adjusting the angular position of the annular mounting frame (401) and the ultrasonic generator (501), the rotating assembly (2) comprising an upper fixed plate (201), a rotating motor (202) being fixedly mounted at the center of the lower end of the upper fixed plate (201), the lower output end of the rotating motor (202) being connected to the upper end pole of the annular mounting frame (401) via a rotating shaft, and a plurality of rotating shafts are fixedly mounted on the side of the rotating shaft. A movable plate (203), a connecting rod (204) is vertically fixedly arranged at the lower end of the rotating plate (203) away from the rotating shaft, the lower end of the connecting rod (204) is connected and fixed to the upper end of the annular mounting frame (401) and the upper end of the connecting plate (304), the lower end of the lower end pole of the annular mounting frame (401) is vertically rotated in a rotating sleeve (206) through a rotating shaft, and the rotating sleeve (206) is fixedly installed at the upper center position of the lower fixed plate (205).

6. The improved biochemical incubator according to claim 1, characterized in that: The culture box also includes a sound-absorbing isolation component (6) for reducing the influence of external vibration or noise on the spherical culture chamber and maintaining the stability of the sound field. The sound-absorbing isolation component (6) is arranged on the outside of the rotating component (2). The sound-absorbing isolation component (6) includes an isolation box (601). The inner wall of the isolation box (601) is connected and fixed to the upper fixing plate (201) and the lower fixing plate (205). The inner wall of the isolation box (601) is made of sound insulation material. The upper end of the side of the isolation box (601) is fitted with a mounting sleeve (602). The side of the mounting sleeve (602) is symmetrically mounted with multiple groups of side shock-absorbing pads (603). The side of the side shock-absorbing pad (603) away from the mounting sleeve (602) is in contact with the box body ( 1), the lower end of the mounting sleeve (602) is provided with a support plate (604), the support plate (604) is fixedly arranged on the side of the isolation box (601), and handles (605) are fixedly installed on the left and right sides of the lower end of the support plate (604), and the lower end of the isolation box (601) is vertically fixed with multiple groups of lower end buffer columns (606), the lower ends of the lower end buffer columns (606) are connected and fixed to the mounting plate (607), and the side of the mounting plate (607) is connected and fixed to the inner wall of the box body (1), the lower end buffer column (606) includes a buffer sleeve and a buffer spring, and multiple groups of high-definition cameras (608) are installed on the side wall and bottom wall of the isolation box (601).

7. The improved biochemical incubator according to claim 1, characterized in that: A box door (7) is installed above the front end of the box body (1), a pull rod (9) is installed on the right side of the box door (7), ventilation holes (11) are symmetrically opened on the upper ends of the left and right sides of the box body (1), a controller (8) is installed at the front end of the box door (7), and an operating status indicator light (10) is installed on the lower end of the controller (8) on the box door (7).

8. The improved biochemical incubator according to claim 1, characterized in that: The front end of the box (1) is provided with a plurality of heat dissipation holes (14) below the mounting plate (607); the lower right end of the box (1) is provided with a power interface (12); a wireless charger is provided inside the box (1) at the lower end of the mounting plate (607); the wireless charger is an electromagnetic induction wireless charger; and a universal wheel (13) is provided at the lower end of the box (1).

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