A bacterial strain antagonistic to multiple pathogens of walnut anthracnose and its application

Abstract

The present invention discloses a bacterial strain antagonistic to multiple pathogens of walnut anthracnose and its application. The present invention provides Serratia ( Serratia sp.) AKX‑27 strain, whose deposit number is CGMCC NO.32361. The present invention also provides the use of the strain or its culture solution or its bacterial solution or its fermentation solution in any of the following: 1) inhibiting pathogens of walnut anthracnose; 2) preventing or treating plant walnut anthracnose; 3) preparing products for preventing or treating plant walnut anthracnose. The strain is expected to become a new choice for the prevention and control of walnut anthracnose, and play a key role in the field of green prevention and control of plant diseases, making important contributions to promoting the sustainable development of agricultural production. As a biological control material for walnut anthracnose, the bacteria have good application prospects whether it is the development of new biological agents or biological pesticides.

Description

Technical Field

[0001] The invention belongs to the field of microbiology and relates to a bacterial strain antagonistic to multiple pathogens of walnut anthracnose and application thereof. Background Art

[0002] Walnut anthracnose, as a major disease in the walnut industry, poses a serious threat to the healthy growth of walnut trees. This disease is caused by Colleotrichum gloeosporioides , Colleotrichum mengyinense and Colleotrichumjuglandium It is caused by a variety of fungi, including necrotic spots on the leaves and fruits of walnuts, which not only reduces the quality of the fruit and the yield, but may also lead to the death of the entire tree in extreme cases.

[0003] At present, chemical control is the main means of controlling walnut anthracnose. Commonly used chemical pesticides, such as various fungicides, reduce the disease by inhibiting the growth and reproduction of pathogens. However, long-term reliance on chemical pesticides for control pollutes the environment, destroys the structure and function of soil microbial communities, and affects soil health and ecological balance. The increasing resistance of pathogens to chemical pesticides has led to a weakening of the effectiveness of some commonly used chemical pesticides and reduced control effectiveness. In addition, the problem of chemical pesticide residues has also caused public concern about food safety.

[0004] Therefore, finding strains for controlling walnut anthracnose for biological control has become a research hotspot. Summary of the invention

[0005] The technical problem solved by the invention is to provide a bacterium capable of antagonizing multiple pathogens of walnut anthracnose, thereby overcoming the problems of environmental pollution and enhanced drug resistance of pathogens caused by chemical pesticides in the prevention and treatment process.

[0006] In order to solve the above technical problems, in the first aspect, the present invention provides Serratia ( Serratia sp.) strain AKX-27, whose deposit number is CGMCC NO.32361.

[0007] In a second aspect, the present invention provides the use of the strain or its culture solution or its bacterial solution or its fermentation solution according to the first aspect in any of the following:

[0008] 1) Inhibit the pathogen of walnut anthracnose;

[0009] 2) Prevent or treat walnut anthracnose;

[0010] 3) Preparation of products for preventing or treating walnut anthracnose.

[0011] In the above application, the pathogen of walnut anthracnose is at least one of the following: C. gloeosporioides, C. mengyinense and C. juglandium .

[0012] In the applications described above, the product is a microbial agent or a biological pesticide.

[0013] In the application described above, the bacterial agent is a biocontrol agent.

[0014] In a third aspect, the present invention provides a product, the active ingredient of which is the strain or its culture solution or its bacterial liquid or its fermentation liquid as described in the first aspect.

[0015] Among the products described above, the product has any of the following functions:

[0016] 1) Inhibit the pathogen of walnut anthracnose;

[0017] 2) Prevent or treat walnut anthracnose in plants.

[0018] In the above-mentioned product, the pathogen of walnut anthracnose is at least one of the following: C. gloeosporioides, C. mengyinense and C. juglandium .

[0019] Among the products mentioned above, the product is a fungicide or a biological pesticide.

[0020] In the product described above, the bacterial agent is a biocontrol agent.

[0021] In the above, the pathogenic bacteria that inhibit walnut anthracnose may be pathogenic bacteria that inhibit walnut anthracnose in a high-salt environment.

[0022] In the above, the plant is a dicotyledon or a monocotyledon;

[0023] In the above, the dicotyledonous plant is a plant of the Juglandaceae family.

[0024] In the above text, the Juglandaceae plant is walnut tree.

[0025] In the above, the plant can grow in high-salt soil or saline-alkali land.

[0026] The present invention provides a new antagonistic bacterial strain - Serratia ( Serratia sp.) AKX-27, which can effectively control various pathogens of walnut anthracnose (caused by Colleotrichum gloeosporioides , Colleotrichum mengyinense and ColleotrichumjuglandiumCaused by). This strain was screened from the natural environment and has high antagonistic activity and stability. Under laboratory conditions, the strain showed significant inhibitory effects on multiple pathogens of walnut anthracnose, providing a new and effective means for the biological control of walnut anthracnose. Based on laboratory research results and theoretical analysis, the strain provides a solid data and theoretical basis for the development and optimization of subsequent field trials. Therefore, this strain is expected to become a new choice for the prevention and control of walnut anthracnose, play a key role in the field of green prevention and control of plant diseases, and make important contributions to promoting the sustainable development of agricultural production. As a biological control material for walnut anthracnose, this bacterium has a good application prospect whether it is the development of new biological agents or biological pesticides.

[0027] Collection Instructions

[0028] Strain name: AKX-27

[0029] Latin name: Serratia sp.

[0030] Depository: China National Microbiological Culture Collection Administration General Microbiology Center

[0031] Abbreviation of depository institution: CGMCC

[0032] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing

[0033] Deposit date: October 25, 2024

[0034] CGMCC Registration Number: CGMCC No.32361

[0035] Classification name: Serratia Serratia sp. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 For Serratia Serratia sp.) AKX-27 colony macroscopic morphology and individual morphology of LB plate culture, among which, Figure 1 A is the macroscopic morphology of AKX-27 colonies; Figure 1 B is the individual morphology diagram of AKX-27.

[0037] Figure 2 For Serratia Serratia sp.) Gram staining results of AKX-27, scale bar is 10 µm.

[0038] Figure 3 For Serratia Serratia sp.) Phylogenetic tree diagram of AKX-27.

[0039] Figure 4 For Serratia Serratia sp.) AKX-27 has antagonistic effects on three pathogens of walnut anthracnose, among which Figure 4 A is walnut anthracnose C . gloeosporioides Pathogen blank control; Figure 4 B is the pathogen of walnut anthracnose AKX-27 C . gloeosporioides antagonistic effect; Figure 4 C is the pathogen of walnut anthracnose C . mengyinense Blank control; Figure 4 D is AKX-27, the pathogen of walnut anthracnose C . mengyinense Antagonistic effect; Figure 4 E is the pathogen of walnut anthracnose C . juglandium Blank control; Figure 4 F is the pathogen of walnut anthracnose AKX-27 Colleotrichumjuglandium antagonistic effect.

[0040] Figure 5 For Serratia Serratia sp.) AKX-27 fermentation culture fluid against three pathogens of walnut anthracnose, among which, Figure 5 A is walnut anthracnose C . gloeosporioides Pathogen blank control; Figure 5 B is the AKX-27 fermentation liquid against walnut anthracnose pathogen C . gloeosporioides antagonistic effect; Figure 5 C is the pathogen of walnut anthracnose C . mengyinense Blank control; Figure 5 D is the AKX-27 fermentation liquid against walnut anthracnose pathogen C . mengyinense antagonistic effect; Figure 5 E is the pathogen of walnut anthracnose C . juglandium Blank control; Figure 5 F is the pathogen of walnut anthracnose AKX-27 Colleotrichumjuglandium antagonistic effect.

[0041] Figure 6 The Serratia serrata cultured under different salt concentrations ( Serratia sp.) AKX-27 is the pathogen of walnut anthracnose C . gloeosporioides The antibacterial activity of Figure 6 A is 2% NaCl concentration C . gloeosporioides Pathogen blank control; Figure 6 B is the walnut anthracnose pathogen AKX-27 cultured under 2% NaCl concentration C . gloeosporioides antagonistic effect; Figure 6 C is 5% NaCl concentration C . gloeosporioides Pathogen blank control; Figure 6 D is the pathogen of walnut anthracnose AKX-27 cultured under 5% NaCl concentration C . gloeosporioides antagonistic effect; Figure 6 E is 7% NaCl concentration C . gloeosporioides Pathogen blank control; Figure 6 F is the walnut anthracnose pathogen AKX-27 cultured under 7% NaCl concentration C . gloeosporioides antagonistic effect; Figure 6 G is 10% NaCl concentration C . gloeosporioides Pathogen blank control; Figure 6 H is 10% NaCl concentration cultured AKX-27 walnut anthracnose pathogen C . gloeosporioides antagonistic effect.

[0042] Figure 7 The Serratia serrata cultured under different salt concentrations ( Serratia sp.) AKX-27 is the pathogen of walnut anthracnose C . mengyinense The antibacterial activity of Figure 7 A is 2% NaCl concentration C . mengyinense Pathogen blank control; Figure 7 B is the walnut anthracnose pathogen AKX-27 cultured under 2% NaCl concentration C . mengyinense antagonistic effect; Figure 7 C is 5% NaCl concentration C . mengyinense Pathogen blank control; Figure 7 D is the pathogen of walnut anthracnose AKX-27 cultured under 5% NaCl concentration C . mengyinense antagonistic effect; Figure 7 E is 7% NaCl concentration C . mengyinense Pathogen blank control; Figure 7 F is the walnut anthracnose pathogen AKX-27 cultured under 7% NaCl concentration C . mengyinense antagonistic effect; Figure 7 G is 10% NaCl concentration C . mengyinense Pathogen blank control; Figure 7 H is 10% NaCl concentration cultured AKX-27 walnut anthracnose pathogen C . mengyinense antagonistic effect.

[0043] Figure 8 The Serratia serrata cultured under different salt concentrations ( Serratia sp.) AKX-27 is the pathogen of walnut anthracnose C . juglandium The antibacterial activity of Figure 8 A is 2% NaCl concentration C . juglandium Pathogen blank control; Figure 8 B is the walnut anthracnose pathogen AKX-27 cultured under 2% NaCl concentration C . juglandium antagonistic effect; Figure 8 C is 5% NaCl concentration C . juglandium Pathogen blank control; Figure 8 D is the pathogen of walnut anthracnose AKX-27 cultured under 5% NaCl concentration C . juglandium antagonistic effect; Figure 8 E is 7% NaCl concentration C . juglandium Pathogen blank control; Figure 8 F is the walnut anthracnose pathogen AKX-27 cultured under 7% NaCl concentration C . juglandium antagonistic effect; Figure 8 G is 10% NaCl concentration C . juglandium Pathogen blank control; Figure 8 H is 10% NaCl concentration cultured AKX-27 walnut anthracnose pathogen C . juglandium antagonistic effect. DETAILED DESCRIPTION

[0044] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0045] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0046] Unless otherwise specified, the quantitative tests in the following examples were performed three times and the results were averaged.

[0047] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods.

[0048] The culture medium preparation method used in the present invention is as follows:

[0049] PDA medium: 200 g peeled potatoes, 20 g glucose, 20 g agar, 1000 mL distilled water.

[0050] LB nutrient agar medium: 10 g tryptone, 5 g yeast extract, 10 g NaCl, 15 g agar, and 1000 mL distilled water.

[0051] LB liquid culture medium: 10 g tryptone, 5 g yeast extract powder, 10 g sodium chloride, and 1000 mL distilled water.

[0052] NA medium: peptone 10 g, sodium chloride 5 g, beef extract powder 3 g, agar 15 g, distilled water 1000 mL.

[0053] TSA medium: 15 g tryptone, 5 g soy peptone, 5 g sodium chloride, 15 g agar, and 1000 mL distilled water.

[0054] Walnut Anthracnose Pathogen C . gloeosporioides , C . mengyinense and C . juglandium Both are reported in the following literature: Li, Y., Lin, L., Cao, J., Gan, M., & Fan, X. (2024). Three newspecies of Colletotrichum (Glomerellales, Glomerellaceae) associated withwalnut (Juglansregia) anthracnose from China. MycoKeys, 108 , 147–167.

[0055] Example 1, Serratia ( Serratia Isolation, screening and identification of AKX-27 from

[0056] 1. Isolation and screening of strain AKX-27

[0057] Soil samples were collected from Honghe Walnut Garden, Erlang Village, Cigou Town, Hanbin District, Ankang City, Shaanxi Province. 5 g of rhizosphere soil from walnut anthracnose-infected areas was placed in a conical flask containing 45 mL of sterile water and sterile steel balls. The flask was placed in a shaker at 30 °C and 180 r / min for 60 min to obtain a bacterial suspension. The bacterial suspension was filtered with sterile gauze and fixed to 100 mL (i.e., 10 -1 The soil dilution was diluted by 10 times gradient dilution to obtain 10 -2 and 10 -3 concentration of soil dilution). From 10 -2 and 10 -3 100 μL of soil dilution with different concentrations was taken out respectively, and applied to LB medium with a sterile applicator. After evenly applying, the mixture was sealed and placed in a culture room at 28°C for inverted culture. After continuous observation for 5 days, single colonies with different morphological characteristics were placed on new LB medium by the plate streak method for purification and culture to obtain soil bacterial strains causing walnut anthracnose, which were stored at 4°C for future use.

[0058] Antagonistic bacteria were screened by plate pairing method, and the antagonistic object was selected as Colletotrichum gloeosporioides, which can cause walnut anthracnose. C . gloeosporioides . Use a 0.4 mm diameter punch to punch the walnut anthracnose pathogen C . gloeosporioides Inoculate on PDA medium, and at the same time inoculate the pure culture isolated above on PDA medium, inoculate 4 test strains on each dish, and form a cross at each point. C . gloeosporioides The blank control was cultured at 28℃. When the control grew all over the plate, the strains with antibacterial effect were selected for rescreening.

[0059] The rescreening was completed on the PDA plate. The specific method was as follows: a 4 mm pathogen block was inoculated in the center of the PDA plate, and the antibacterial strains obtained from the above primary screening were inoculated on both sides of the pathogen block 2 cm away using a plate. The three walnut anthracnose pathogens ( C . gloeosporioides , C . mengyinense and C . juglandium ) were used as blank controls, and each treatment was repeated three times. The plates were cultured at 28°C. When the control grew all over the plate, the antagonistic strain AKX-27 with the strongest antibacterial effect was selected.

[0060] 2. Identification of strain AKX-27

[0061] 1) Morphological identification

[0062] The strain AKX-27 was cultured on LB plates, NA plates, and TSA plates.

[0063] The colonies of AKX-27 on LB plates are white, round, convex in the middle, opaque, and have irregular edges. The culture medium is white ( Figure 1 ). The strain AKX-27 can also grow well on NA and TSA plates.

[0064] The Gram reaction of strain AKX-27 was negative ( Figure 2 ), the bacteria are short rod-shaped and mobile.

[0065] 2) Identification of physiological and biochemical characteristics

[0066] The antagonistic bacteria AKX-27 is a facultative anaerobic bacterium; the growth temperature is 20℃-40℃, the optimal culture temperature is 32℃, the optimal culture time is 48h, and it is a heat-resistant bacterium; it can grow well in LB medium with a NaCl concentration of 2%-10%; it has the ability to liquefy gelatin and can hydrolyze starch.

[0067] The physiological and biochemical characteristics of AKX-27 are shown in Table 1.

[0068]

[0069] Note: +, positive reaction or can be utilized, growth; -, negative reaction or cannot grow, utilization.

[0070] 3) Molecular identification

[0071] Pick a small amount of AKX-27 single colony and put it into a 2 mL sterile centrifuge tube containing LB liquid culture medium, place it in a shaker at 30°C, 180 r / min constant temperature and culture for 48 hours, aspirate 200 μL into a 1.5 mL sterile centrifuge tube, bathe in 100°C water, centrifuge for 20 minutes, take the supernatant, which is the DNA of strain AKX-27, and store it at 4°C.

[0072] 16S was used for molecular biological determination. The PCR reaction system (25 μL) included 1 μL of each primer, 12.5 μL of 2x Mix, 2 μL of DNA template, and ddH2O was added to make up to 25 μL. The universal primers used were 27F / 1492R, 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-GGTTACCTTGTTACGACTT-3'. The reaction procedure was: 94°C pre-denaturation for 5 min, 94°C denaturation for 30 s, 55°C annealing for 30 s, 72°C extension for 45 s, 72°C extension for 10 min, and 4°C storage after 30 cycles.

[0073] The 1298 bp PCR product was sent to Beijing Nosai Genome Research Center Co., Ltd. for 16SrDNA sequencing. The nucleotide sequence of 16SrDNA was sequence 1.

[0074] The above sequence 1 was aligned and cut using MEGA 6.0, and phylogenetic analysis was performed using the maximum likelihood method and Bayesian method. The results are as follows Figure 3 As shown, Serratia liquefaciens (ATCC 12926 T) has the highest homology.

[0075] The above molecular biological determination, morphological characteristics and physiological and biochemical characteristics were comprehensively identified. The results showed that strain AKX-27 was a new species of the genus Serratia, which was identified as: Serratia sp.

[0076] The strain AKX-27 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC) on October 25, 2024, at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the deposit number is CGMCC No. 32361, and the classification name is Serratia Serratia sp ..

[0077] Example 2, Serratia ( Serratia Application of AKX-27 in inhibiting three pathogens of walnut anthracnose

[0078] The fungal blocks of the three pathogens of walnut anthracnose mentioned below are the fungal blocks taken when the three pathogens are cultured in PDA culture medium until they fill the entire dish.

[0079] 1. Serratia Serratia Antagonistic effect of AKX-27 on three pathogens of walnut anthracnose

[0080] A single colony of the strain AKX-27 obtained in Example 1 was picked and placed in a 2 mL sterile centrifuge tube containing LB liquid culture medium and a sterile filter paper, and placed in a shaker at 30°C and 180 r / min for 48 h to prepare a filter paper containing the strain AKX-27.

[0081] A 4 mm block of walnut anthracnose pathogen was inoculated in the center of the PDA plate, and the filter paper containing strain AKX-27 was inoculated on both sides of the pathogen block 2 cm away by using the plate confrontation method and plate streaking to serve as the treatment group (AKX-27). C . gloeosporioides , C . mengyinense and C . juglandium) plates were used as blank controls (CK) and recorded as control groups. Each treatment was repeated three times. The plates were cultured at 28°C. When the control cells grew all over the plate, the relative inhibition rate was calculated.

[0082] The results are as follows Figure 4 As shown, it can be seen that AKX-27 has antagonistic effects on three pathogens of walnut anthracnose.

[0083] Serratia Serratia sp.) AKX-27 against three pathogens of walnut anthracnose is shown in Table 2.

[0084] The inhibition rate was calculated using the following method: relative inhibition rate = (colony diameter of the control group - colony diameter of the treatment group) / colony diameter of the control group * 100%

[0085] Colony diameter = mycelium diameter - fungus block diameter, using the cross method.

[0086]

[0087] The above results all indicate that AKX-27 inhibits three pathogens of walnut anthracnose.

[0088] 2. Serratia Serratia sp.) AKX-27 fermentation culture fluid against three pathogens of walnut anthracnose

[0089] A single colony of AKX-27 obtained in Example 1 was picked and placed in a 2 mL sterile centrifuge tube containing LB liquid culture medium, and placed in a shaker at 30°C, 180 r / min constant temperature shaking culture for 48 h, and the OD (600) value was ≈1.2, to prepare an AKX-27 sterile fermentation liquid.

[0090] AKX-27 sterile fermentation liquid and PDA medium were mixed evenly in a volume ratio of 1:10 and poured into plates. After the plates were dried, walnut anthracnose pathogenic bacteria blocks with a diameter of 4 mm were placed on the plates (recorded as the treatment group, AKX-27). Sterile water and PDA medium were mixed in a volume ratio of 1:10 and poured into plates as blank controls (recorded as the control group). Repeat three times. When the control fills the entire plate, the relative inhibition rate is calculated.

[0091] The results are as follows Figure 5 As shown, it can be seen that the AKX-27 sterile fermentation broth has an inhibitory effect on the three pathogens of walnut anthracnose.

[0092] Serratia Serratia sp.) AKX-27 fermentation culture fluid against three walnut anthracnose pathogens are shown in Table 3.

[0093] The inhibition rate was calculated using the following method: relative inhibition rate = (colony diameter of the control group - colony diameter of the treatment group) / colony diameter of the control group * 100%

[0094] Colony diameter = mycelium diameter - fungus block diameter, using the cross method.

[0095]

[0096] The above results showed that the aseptic fermentation broth of AKX-27 inhibited three pathogens of walnut anthracnose.

[0097] 3. Serratia marcescens at different salt concentrations ( Serratia sp.) AKX-27 against three pathogens of walnut anthracnose

[0098] LB liquid culture medium with different salt concentrations (mass volume percentage g / ml, 2%, 5%, 7%, 10%) was prepared, and a single colony of AKX-27 was picked and placed in 2 mL sterile centrifuge tubes containing LB liquid culture medium with different salt concentrations (2%, 5%, 7%, 10%) and sterile filter paper, and placed in a shaker at 30°C, 180r / min for 48 hours to prepare bacterial suspensions of AKX-27 with different salt concentrations.

[0099] The plate confrontation method was used to inoculate three pathogenic bacteria blocks of 4 mm in size in the center of the PDA plate. At the same time, filter paper containing AKX-27 with different salt concentrations (2%, 5%, 7%, and 10%) was inoculated on both sides of the pathogenic bacteria block at 2 cm by streaking the plate (recorded as the treatment group, AKX-27); sterile water with different salt concentrations (2%, 5%, 7%, and 10%) was inoculated on both sides of the pathogenic bacteria block at 2 cm by streaking the plate as a blank control (recorded as the control group), and repeated three times. When the control fills the entire plate, the relative inhibition rate is calculated.

[0100] The results of AKX-27 bacterial solution with different salt concentrations on different pathogens are as follows Figure 6 , Figure 7 and Figure 8 As shown, it can be seen that as the salt concentration increases, the antagonistic effect of AKX-27 decreases, so the higher the salt concentration, the lower the antagonistic activity of AKX-27, but it still has a significant inhibitory effect on the three pathogens of walnut anthracnose.

[0101] Serratia Serratia sp.) against three pathogens of walnut anthracnose as shown in Table 4.

[0102] The inhibition rate was calculated using the following method: relative inhibition rate = (colony diameter of the control group - colony diameter of the treatment group) / colony diameter of the control group * 100%

[0103] Colony diameter = mycelium diameter - fungus block diameter, using the cross method.

[0104]

[0105] The results showed that strain AKX-27, AKX-27 fermentation broth and AKX-27 bacterial broth with different salt concentrations all had significant inhibitory effects on the three pathogens of walnut anthracnose.

[0106] These examples are only used to illustrate the technical solutions of the present invention and are not intended to impose limitations thereon. Although the present invention has been described in detail based on the above examples, professionals in this field can still adjust the technical solutions recorded in the examples or make equivalent substitutions for certain technical elements without departing from the protection scope of the present invention. These adjustments or substitutions will not change the essence of the corresponding technical solutions and will still comply with the protection objectives and scope pursued by the present invention.

[0107] The present invention has been described in detail above. It will be apparent to those skilled in the art that the present invention may be implemented in a wide range under equivalent parameters, concentrations and conditions without departing from the spirit and scope of the present invention and without the need for unnecessary experimentation. Although the present invention provides specific embodiments, it should be understood that further improvements may be made to the present invention. In short, according to the principles of the present invention, this application intends to include any changes, uses or improvements to the present invention, including changes made by conventional techniques known in the art that depart from the scope disclosed in this application. Applications of some of the basic features may be made within the scope of the following appended claims.

Claims

1. Serratia marcescens ( Serratia sp.) strain AKX-27, whose deposit number is CGMCC NO.32361.

2. Use of the strain according to claim 1 or its culture solution or its bacterial solution or its fermentation solution in any of the following: 1) Inhibit the pathogen of walnut anthracnose; 2) Prevent or treat walnut anthracnose; 3) Preparation of products for preventing or treating walnut anthracnose; The pathogen of walnut anthracnose is at least one of the following: C. gloeosporioides, C. mengyinense and C. juglandium .

3. The use according to claim 2, characterized in that: The product is a microbial agent or a biological pesticide.

4. The use according to claim 3, characterized in that: The bacterial agent is a biocontrol agent.

5. A product, the active ingredient of which is the strain according to claim 1 or its culture solution or bacterial liquid or fermentation liquid.

6. The product according to claim 5, characterized in that: The product has any of the following functions: 1) Inhibit the pathogen of walnut anthracnose; 2) Prevent or treat walnut anthracnose; The pathogen of walnut anthracnose is at least one of the following: C. gloeosporioides, C. mengyinense and C. juglandium .

7. The product according to claim 5 or 6, characterized in that: The product is a microbial agent or a biological pesticide.

8. The product according to claim 6, characterized in that: The product is a bacterial agent; The bacterial agent is a biocontrol agent.

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