Composition for warming and invigorating kidney yang and enhancing immunity as well as preparation method and application of composition

Abstract

The invention discloses a composition for warming and invigorating kidney yang and enhancing immunity as well as a preparation method and application of the composition for warming and invigorating kidney yang and enhancing immunity. Comprising the following raw materials in parts by weight: cornus wilsoniana fruit oil, rhus chinensis fruit oil, a willow herb extract, a melastoma dodecandrum lour extract, green tea, cinnamon, Chinese wolfberry fruits, raspberries, mulberries, astragalus membranaceus, rhizoma polygonati, herba epimedii, cistanche deserticola, morinda officinalis and isomaltitol. The composition acts on the injured part of the human body through the specific active ingredients, and has the effects of warming and invigorating kidney yang and improving the immunity of the human body.

Description

Technical Field

[0001] The invention relates to the technical field of health food, and in particular to a composition for warming and nourishing kidney yang and enhancing immunity, and a preparation method and application thereof. Background Art

[0002] Kidney Yang deficiency syndrome refers to a syndrome characterized by kidney Yang deficiency, loss of warmth in the body, and mainly manifested by soreness and coldness in the waist and knees, loss of libido, frequent urination at night, and symptoms of Yang deficiency. Symptoms: soreness and coldness in the waist and knees, fear of cold and cold limbs, especially in the lower limbs, pale or dark complexion, fatigue and weakness; or sexual apathy, impotence, spermatorrhea, premature ejaculation in men, infertility due to uterine coldness, and clear and thin leucorrhea in women; or frequent and clear urination, frequent urination at night, pale tongue with white fur, deep and weak pulse, especially in the radial artery. Kidney Yang is the foundation of the Yang Qi of each organ, and it promotes and warms each organ and tissue. When kidney Yang is sufficient, the body's functions are vigorous. When kidney Yang is deficient, the organs lose warmth and their functions are low, which brings many inconveniences to people's lives and affects their quality of life.

[0003] The immune system is mainly composed of two mechanisms: innate immunity and acquired immunity. The former means that when a virus or bacteria invades the human body, it will respond quickly and produce antibodies against the virus to attack it. The latter means that once infected with a certain virus or bacteria, it will remember the characteristics of the virus or bacteria and prepare for the next invasion. Immune cells are responsible for this innate immunity and acquired immunity. The current preparation composed of Curculigo, Epimedium, Morinda, Angelica, Phellodendron, and Anemarrhena has the functions of warming kidney yang, replenishing kidney essence, connecting the heart and kidney, and regulating, but no mention is made of the regulation of immunity.

[0004] There are not many oral Chinese medicine products for warming and nourishing kidney yang on the market, and a few existing products still have illegal Western medicine ingredients added. After oral administration, Western medicines for warming and nourishing kidney yang are weakened by gastrointestinal digestive juices and other effects, and will irritate the gastrointestinal tract, affect digestive function, and cause adverse reactions to the liver. Therefore, it is very necessary to develop a Chinese medicine warming and nourishing kidney yang composition that has both the effect of warming and nourishing kidney yang and is easy to use, safe, and has no toxic side effects. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the present invention proposes a composition for warming and nourishing kidney yang and enhancing immunity, and a preparation method and application thereof, to solve the problems raised by the above-mentioned background technology.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 10-20 parts of elm truncatum fruit oil, 10-18 parts of rhus chinensis fruit oil, 8-15 parts of angustifolia extract, 7-12 parts of melaleuca extract, 6-11 parts of green tea, 6-10 parts of cinnamon bark, 4-8 parts of wolfberry, 4-8 parts of raspberry, 3-7 parts of mulberry, 3-6 parts of astragalus, 2-5 parts of polygonatum, 2-4 parts of epimedium, 1-4 parts of cistanche, 1-5 parts of morinda officinalis, and 1-3 parts of isomalt.

[0008] Preferably, a composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of Swidawlsonia na (Wanger.) Sojak fruit oil, 15 parts of Rhuschinensis Mill. fruit oil, 12 parts of Epilobium angustifolium extract (a perennial and sturdy herb of the genus Epilobium of the Onagraceae family), 10 parts of Melastoma dodecandrum Lour extract (the fruit of the Melastoma dodecandrum Lour of the Melastomataceae family), 9 parts of green tea, 9 parts of cinnamon, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of Cistanche deserticola, 3 parts of Morinda officinalis, and 2 parts of isomalt.

[0009] Preferably, the preparation method of the angustifolia extract comprises the following steps: drying and crushing the angustifolia, mixing it with ethyl acetate, and performing ultrasonic pretreatment; extracting the pretreated material with supercritical carbon dioxide fluid and peony seed oil at a temperature of 40 to 50°C and a pressure of 20 to 25 MPa for 100 to 120 minutes to obtain an extract; and concentrating the extract under reduced pressure to a relative density of 1.35 to 1.40 to obtain an angustifolia extract.

[0010] Preferably, the mass ratio of angustifolia to ethyl acetate is 1:(8-12), and the weight ratio of the pretreated material to peony seed oil is 1:(0.4-0.9).

[0011] Preferably, the ultrasonic power in the ultrasonic pretreatment is 280-450 W, and the ultrasonic treatment time is 20-60 min.

[0012] Preferably, the flow rate of the supercritical carbon dioxide fluid is 15 to 25 L / hour.

[0013] Preferably, the method for preparing the radix spatholobi extract comprises the following steps:

[0014] S1. Wash fresh radish and place it in water, grind the radish in the water, wherein the ratio of radish to water is 1:(20-25) by mass, and place the water container in an ice water bath to obtain radish grinding liquid;

[0015] S2, adding 0.1-1 mol / L HCl solution to the ground radix schizonepetae to adjust the pH to 4.5-6.5, and then adding 0.1-1.5% of the total weight of α-amylase and 2-5% of saccharifying enzyme for enzymolysis for 4-7 hours to obtain an enzymolysis solution;

[0016] S3, placing the enzymatic hydrolyzate obtained in step S2 in a 95-100°C water bath to inactivate the enzyme for 20-30 minutes;

[0017] S4, placing the enzyme-killed hydrolyzate obtained in step S3 at a preset temperature of 0-4°C for refrigerated centrifugation, maintaining the speed at 5000-7000 r / min, collecting the upper clear liquid, filtering the clear liquid through a 0.3-0.6 μm filter element, and drying to obtain the radix schizonepetae extract.

[0018] Preferably, the α-amylase activity is 20,000 U / g, and the saccharifying enzyme activity is 100,000 U / g.

[0019] Preferably, a method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0020] (1) Grinding: Dry and grind green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche and Morinda officinalis, and separate and sieve through 100-200 mesh to obtain a mixed dry powder for later use;

[0021] (2) Enzymatic hydrolysis: Adding the fruit oil of Elaeagnus glabra, the fruit oil of Rhus chinensis, the extract of Epilobium angustifolium, the extract of Elaeagnus oleifera and isomalt to the mixed dry powder and stirring evenly to obtain a mixed solution; adjusting the pH value of the mixed solution to 6.0-6.5, adding a composite enzyme, and performing enzymatic hydrolysis at a temperature of 35-45° C. for 4-7 hours to obtain an enzymatic hydrolyzate;

[0022] (3) Inactivating the enzyme: placing the enzymatic solution obtained in step (2) at a temperature of 110 to 120° C. to inactivate the enzyme for 12 to 20 minutes;

[0023] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 3000-4500 rpm for 8-15 min and collect the supernatant;

[0024] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0025] Preferably, the compound enzyme is a compound ratio of tannase to xylanase in a mass ratio of 1:(2-4), and the added amount of the compound enzyme is 0.5-1.5% of the total mass of the mixed solution.

[0026] Preferably, the tannase activity is 100,000 U / g, and the xylanase activity is 50,000 U / g.

[0027] Preferably, the composition is used in the preparation of functional foods for warming and nourishing kidney yang and enhancing immunity.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) The main ingredients of the composition for warming and nourishing kidney yang and enhancing immunity are elm oil, rhus chinensis fruit oil, angustifolia extract, and physalis extract. The active ingredients contained in the composition, such as lauric acid, myristic acid, palmitic acid, quercetin, and isorhamnetin, can quickly penetrate into the kidneys, promote the normal operation of internal organs, regulate the balance of the endocrine system and the nervous system, and maintain the stability of the human body.

[0030] (2) Supercritical carbon dioxide fluid and peony seed oil are used to extract the angustifolia extract. Peony seed oil is used as a plant entrainer, which not only significantly improves the extraction efficiency, but also has the advantages of being safe, healthy, environmentally friendly, residue-free, simple and cheap.

[0031] (3) Cold grinding + acid enzymatic hydrolysis technology is used to obtain the extract of the radix schizonepetae. The radix schizonepetae cells are broken by grinding and the active small molecules are released. The solid and liquid phases are separated by centrifugation. The active ingredients such as steroidal triterpenes, saponins, flavonoids, lactones, alkaloids, amino acids, peptides, sugars and polysaccharides are released by enzymatic hydrolysis to improve human immunity. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0033] Example 1

[0034] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 10 parts of safflower oil, 10 parts of rhus chinensis fruit oil, 8 parts of angustifolia extract, 7 parts of kelp extract, 6 parts of green tea, 6 parts of cinnamon bark, 4 parts of wolfberry, 4 parts of raspberry, 3 parts of mulberry, 3 parts of astragalus, 2 parts of polygonatum, 2 parts of epimedium, 1 part of cistanche, 1 part of morinda officinalis, and 1 part of isomalt.

[0035] The preparation method of an epilobium angustifolium extract comprises the following steps: drying and crushing the epilobium angustifolium, mixing the epilobium angustifolium with ethyl acetate at a material-liquid mass ratio of 1:8, and performing ultrasonic pretreatment for 20 minutes at an ultrasonic power of 280W; extracting the pretreated material with a supercritical carbon dioxide fluid at a flow rate of 15L / hour and peony seed oil at a temperature of 40°C and a pressure of 20Mpa for 100 minutes to obtain an extract; and concentrating the extract under reduced pressure to a relative density of 1.35 to obtain an epilobium angustifolium extract; wherein the weight ratio of the pretreated material to the peony seed oil is 1:0.4.

[0036] The preparation method of the radix spatholobi extract comprises the following steps:

[0037] S1, taking fresh radish, washing it and placing it in water, grinding the radish in the water, wherein the ratio of radish to water is 1:20 by mass, and the water container is placed in an ice water bath throughout the process to obtain a radish grinding liquid;

[0038] S2, adding 0.1 mol / L HCl solution to the ground radix schizonepetae to adjust the pH to 4.5, and then adding 0.1% of the total weight of α-amylase and 2% of saccharifying enzyme for enzymolysis for 4 hours to obtain an enzymolysis solution;

[0039] S3, placing the enzymatic hydrolyzate obtained in step S2 in a 95°C water bath to inactivate the enzyme for 20 min;

[0040] S4, placing the enzyme-killed hydrolyzate obtained in step S3 at a preset temperature of 0°C for refrigerated centrifugation, maintaining the speed at 5000 r / min, collecting the upper clear liquid, filtering the clear liquid through a 0.3 μm filter element, and drying to obtain the Herba Lysimachiae extract.

[0041] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0042] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 100 mesh to obtain a mixed dry powder for later use;

[0043] (2) Enzymatic hydrolysis: add tanna scabra fruit oil, rhus chinensis fruit oil, angustifolia extract, melaleuca extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; after adjusting the pH value of the mixed solution to 6.0, add a composite enzyme, and perform enzymatic hydrolysis at a hydrolysis temperature of 35° C. for 4 h to obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tannase and xylanase of 1:2 by mass, and the amount of the composite enzyme added is 0.5% of the total mass of the mixed solution.

[0044] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 110°C to inactivate enzyme for 12 min;

[0045] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 3000 rpm for 8 min and collect the supernatant;

[0046] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0047] Example 2

[0048] 1. A composition for warming and nourishing kidney yang and enhancing immunity, comprising the following raw materials in parts by weight: 20 parts of safflower oil, 18 parts of rhus chinensis fruit oil, 15 parts of angustifolia extract, 12 parts of radix schizonepetae extract, 11 parts of green tea, 10 parts of cinnamon, 8 parts of wolfberry, 8 parts of raspberry, 7 parts of mulberry, 6 parts of astragalus, 5 parts of polygonatum, 4 parts of epimedium, 4 parts of cistanche, 5 parts of morinda officinalis, and 3 parts of isomalt.

[0049] The preparation method of an epilobium angustifolium extract comprises the following steps: drying and crushing the epilobium angustifolium, mixing the epilobium angustifolium with ethyl acetate at a material-liquid mass ratio of 1:12, and performing ultrasonic pretreatment for 60 minutes at an ultrasonic power of 450W; extracting the pretreated material with a supercritical carbon dioxide fluid at a flow rate of 25L / hour and peony seed oil at a temperature of 50°C and a pressure of 25Mpa for 120 minutes to obtain an extract; and decompressing and concentrating the extract to a relative density of 1.40 to obtain an epilobium angustifolium extract; wherein the weight ratio of the pretreated material to the peony seed oil is 1:0.9.

[0050] The preparation method of the radix spatholobi extract comprises the following steps:

[0051] S1, washing fresh radish and placing it in water, grinding the radish in the water, wherein the ratio of radish to water is 1:25 by mass, and the water container is placed in an ice water bath throughout the process to obtain a radish grinding liquid;

[0052] S2, adding 1 mol / L HCl solution to the ground radix schizonepetae to adjust the pH to 6.5, and then adding 1.5% of the total weight of α-amylase and 5% of saccharifying enzyme for enzymolysis for 7 hours to obtain an enzymolysis solution;

[0053] S3, placing the enzymatic hydrolyzate obtained in step S2 in a 100°C water bath to inactivate the enzyme for 30 min;

[0054] S4, placing the enzyme-killed hydrolyzate obtained in step S3 at a preset temperature of 4° C. for refrigerated centrifugation, maintaining the speed at 7000 r / min, collecting the upper clear liquid, filtering the clear liquid through a 0.6 μm filter element, and drying to obtain the Herba Lysimachiae extract.

[0055] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0056] (1) Grinding: Dry and grind green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche and Morinda officinalis, and sieve through 200 mesh to obtain a mixed dry powder for later use;

[0057] (2) Enzymatic hydrolysis: add tanna scabra fruit oil, rhus chinensis fruit oil, angustifolia extract, melaleuca extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.5 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 45° C. for 7 h to obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tannase and xylanase of 1:4 by mass, and the amount of the composite enzyme added is 1.5% of the total mass of the mixed solution;

[0058] (3) Inactivating the enzyme: placing the enzymatic solution obtained in step (2) at a temperature of 120° C. to inactivate the enzyme for 20 min;

[0059] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4500 rpm for 15 min and collect the supernatant;

[0060] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0061] Example 3

[0062] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of truncatum glabra fruit oil, 15 parts of rhus chinensis fruit oil, 12 parts of angustifolia extract, 10 parts of melaleuca extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

[0063] The preparation method of an epilobium angustifolium extract comprises the following steps: drying and crushing the epilobium angustifolium, mixing the epilobium angustifolium with ethyl acetate at a material-liquid mass ratio of 1:10, and performing ultrasonic pretreatment for 40 minutes at an ultrasonic power of 300 W; extracting the pretreated material with a supercritical carbon dioxide fluid with a flow rate of 20 L / hour and peony seed oil at a temperature of 45 DEG C and a pressure of 22 MPa for 110 minutes to obtain an extract; and decompressing and concentrating the extract to a relative density of 1.38 to obtain an epilobium angustifolium extract; wherein the weight ratio of the pretreated material to the peony seed oil is 1:0.7.

[0064] A composition for warming and nourishing kidney yang and enhancing immunity, the preparation method of the extract of Radix Astragali comprises the following steps:

[0065] S1, washing fresh radish and placing it in water, grinding the radish in the water, wherein the ratio of radish to water is 1:22 by mass, and the water container is placed in an ice water bath throughout the process to obtain a radish grinding liquid;

[0066] S2, adding 0.8 mol / L HCl solution to the ground radix schizonepetae to adjust the pH to 5.0, and then adding 1% of the total mass of α-amylase and 3% of saccharifying enzyme for enzymolysis for 5 h to obtain an enzymolysis solution;

[0067] S3, placing the enzymatic solution obtained in step S2 in a 98°C water bath to inactivate the enzyme for 25 min;

[0068] S4, placing the enzyme-killed hydrolyzate obtained in step S3 at a preset temperature of 2° C. for refrigerated centrifugation, maintaining the speed at 6000 r / min, collecting the upper clear liquid, filtering the clear liquid through a 0.5 μm filter element, and drying to obtain the Herba Lysimachiae extract.

[0069] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0070] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 180 mesh to obtain a mixed dry powder for later use;

[0071] (2) Enzymatic hydrolysis: add tanna scabra fruit oil, rhus chinensis fruit oil, angustifolia extract, melaleuca extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.1 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 40° C. for 5 h, and obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tannase and xylanase of 1:3 by mass, and the amount of the composite enzyme added is 1.2% of the total mass of the mixed solution;

[0072] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 115°C to inactivate enzyme for 18 min;

[0073] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4000 rpm for 10 min and collect the supernatant;

[0074] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0075] Comparative Example 1

[0076] The difference between this comparative example and Example 3 is that no elm oil is added to the raw materials, and the other ingredients and steps remain unchanged.

[0077] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of Rhus chinensis fruit oil, 12 parts of angustifolia extract, 10 parts of radix schizonepetae extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

[0078] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0079] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 180 mesh to obtain a mixed dry powder for later use;

[0080] (2) Enzymatic hydrolysis: add Rhus chinensis oil, Epilobium angustifolium extract, Eleutherodactylum extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.1 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 40° C. for 5 h, and obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tanninase and xylanase of 1:3 by mass, and the amount of the composite enzyme added is 1.2% of the total mass of the mixed solution;

[0081] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 115°C to inactivate enzyme for 18 min;

[0082] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4000 rpm for 10 min and collect the supernatant;

[0083] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0084] Comparative Example 2

[0085] The difference between this comparative example and Example 3 is that no Rhus chinensis oil is added to the raw materials, and the other ingredients and steps remain unchanged.

[0086] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of smilax china fruit oil, 12 parts of angustifolia extract, 10 parts of radix schizonepetae extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

[0087] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0088] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 180 mesh to obtain a mixed dry powder for later use;

[0089] (2) Enzymatic hydrolysis: add elm oil, angustifolia extract, radix serrata extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.1 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 40° C. for 5 h, and obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tannin and xylanase of 1:3 by mass, and the amount of the composite enzyme added is 1.2% of the total mass of the mixed solution;

[0090] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 115°C to inactivate enzyme for 18 min;

[0091] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4000 rpm for 10 min and collect the supernatant;

[0092] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0093] Comparative Example 3

[0094] The difference between this comparative example and Example 3 is that the preparation method of the angustifolia extract is changed by replacing the peony seed oil with an ethanol aqueous solution, and the other components and steps remain unchanged.

[0095] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of truncatum glabra fruit oil, 15 parts of rhus chinensis fruit oil, 12 parts of angustifolia extract, 10 parts of melaleuca extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

[0096] The preparation method of an epilobium angustifolium extract comprises the following steps: drying and crushing the epilobium angustifolium, mixing the epilobium angustifolium with ethyl acetate at a material-liquid mass ratio of 1:10, and performing ultrasonic pretreatment for 40 minutes at an ultrasonic power of 300 W; extracting the pretreated material with a supercritical carbon dioxide fluid with a flow rate of 20 L / h and an ethanol aqueous solution with a volume fraction of 85% at a temperature of 45 DEG C and a pressure of 22 Mpa for 110 minutes to obtain an extract; and concentrating the extract under reduced pressure to a relative density of 1.38 to obtain an epilobium angustifolium extract; wherein the weight ratio of the pretreated material to peony seed oil is 1:0.7.

[0097] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0098] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 180 mesh to obtain a mixed dry powder for later use;

[0099] (2) Enzymatic hydrolysis: add tanna scabra fruit oil, rhus chinensis fruit oil, angustifolia extract, melaleuca extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.1 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 40° C. for 5 h, and obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tannase and xylanase of 1:3 by mass, and the amount of the composite enzyme added is 1.2% of the total mass of the mixed solution;

[0100] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 115°C to inactivate enzyme for 18 min;

[0101] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4000 rpm for 10 min and collect the supernatant;

[0102] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0103] Comparative Example 4

[0104] The difference between this comparative example and Example 3 is that the preparation method of the radix schizonepetae extract is changed, the radix schizonepetae grinded liquid is prepared at room temperature of 26° C. and centrifuged, and the other components and steps remain unchanged.

[0105] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of truncatum glabra fruit oil, 15 parts of rhus chinensis fruit oil, 12 parts of angustifolia extract, 10 parts of melaleuca extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

[0106] A composition for warming and nourishing kidney yang and enhancing immunity, the preparation method of the extract of Radix Astragali comprises the following steps:

[0107] S1, washing fresh radish, placing it in water, grinding the radish in the water, wherein the ratio of radish to water is 1:22 by mass, and preparing a radish grinding liquid at a temperature of 26° C.;

[0108] S2, adding 0.8 mol / L HCl solution to the ground radix schizonepetae to adjust the pH to 5.0, and then adding 1% of the total mass of α-amylase and 3% of saccharifying enzyme for enzymolysis for 5 h to obtain an enzymolysis solution;

[0109] S3, placing the enzymatic solution obtained in step S2 in a 98°C water bath to inactivate the enzyme for 25 min;

[0110] S4, placing the enzyme-killed hydrolyzate obtained in step S3 at 26° C. for centrifugation, maintaining the rotation speed at 6000 r / min, collecting the upper clear liquid, filtering the clear liquid through a 0.5 μm filter element, and drying to obtain the Eleutherodactyl extract.

[0111] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0112] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 180 mesh to obtain a mixed dry powder for later use;

[0113] (2) Enzymatic hydrolysis: add tanna scabra fruit oil, rhus chinensis fruit oil, angustifolia extract, melaleuca extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.1 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 40° C. for 5 h, and obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of tannase and xylanase of 1:3 by mass, and the amount of the composite enzyme added is 1.2% of the total mass of the mixed solution;

[0114] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 115°C to inactivate enzyme for 18 min;

[0115] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4000 rpm for 10 min and collect the supernatant;

[0116] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0117] Comparative Example 5

[0118] The difference between this comparative example and Example 3 is that the complex enzyme is changed, and the other components and steps remain unchanged.

[0119] A composition for warming and nourishing kidney yang and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of truncatum glabra fruit oil, 15 parts of rhus chinensis fruit oil, 12 parts of angustifolia extract, 10 parts of melaleuca extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

[0120] A method for preparing a composition for warming and tonifying kidney yang and enhancing immunity comprises the following steps:

[0121] (1) Grinding: drying green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche deserticola and Morinda officinalis, and then grinding them, and sieving through 180 mesh to obtain a mixed dry powder for later use;

[0122] (2) Enzymatic hydrolysis: add elm oil, rhus chinensis fruit oil, angustifolia extract, radix elegans extract and isomalt to the mixed dry powder and stir evenly to obtain a mixed solution; adjust the pH value of the mixed solution to 6.1 and then add a composite enzyme, perform enzymatic hydrolysis at a hydrolysis temperature of 40° C. for 5 h, and obtain an enzymatic hydrolyzate; wherein the composite enzyme is a composite ratio of cellulase and pectinase of 1:3 by mass, and the amount of the composite enzyme added is 1.2% of the total mass of the mixed solution;

[0123] (3) Inactivation of enzyme: The enzymatic hydrolyzate obtained in step (2) was placed at 115°C to inactivate enzyme for 18 min;

[0124] (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 4000 rpm for 10 min and collect the supernatant;

[0125] (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

[0126] Experimental Example 1 Acute toxicity determination

[0127] 80 ICR mice, half male and half female, were randomly divided into 8 groups, 10 mice in each group, and the compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 5 were prepared into 6.0 g (composition) / ml solution, which is the maximum concentration that can be given to mice, to ensure that each group of solutions can pass through the No. 16 mouse gavage needle. Each group of mice fasted but not watered for 18 hours before the experiment, and the dosage of the composition was 0.5 ml / 10 g. Immediately after the composition was given, the poisoning symptoms (mental state, motor function, etc.) and death of the animals were observed and recorded, and the observation period was 7 days.

[0128] The results are shown in Table 1:

[0129] Table 1 Acute toxicity determination

[0130] Group Number of mice that died mortality rate Example 1 Group 0 0％ Example 2 Group 0 0％ Example 3 Group 0 0％ Comparative Example 1 0 0％ Comparative Example 2 0 0％ Comparative Example 3 3 30％ Comparative Example 4 0 0％ Comparative Example 5 0 0％

[0131] Except for the comparative example 3 group, the mortality rate of the other groups was 0%, indicating that the composition of each group in the technical scheme is safe; the comparative example 3 group is a change in the preparation method of the angustifolia extract, replacing the peony seed oil with an ethanol aqueous solution, and the poisoning symptoms mainly include dyspnea, shortness of breath and proneness. It is speculated that during the preparation of the angustifolia extract, the ethanol aqueous solution was not completely evaporated and the residue remained in the composition sample, resulting in the death of some mice.

[0132] Experimental Example 2: Effect of Warming and Replenishing Kidney Yang

[0133] 2.1 Experimental Materials

[0134] The compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 5; hydrocortisone injection (manufacturer: Tianjin Jinyao Pharmaceutical Co., Ltd.); Jisheng Shenqi Pills (manufacturer: Baoding Traditional Chinese Medicine Pharmaceutical Co., Ltd., ingredients: Plantago, Poria, Aconite, Paeonia Suffruticosa, Achyranthes Bidentata, Cinnamon Bark, Dioscorea Opposita, Cornus Officinalis, Rehmannia Glutinosa, Alisma Orientalis).

[0135] 2.2 Reagents and kits

[0136] Glacial acetic acid (AR, Hangzhou Chemical Reagent Co., Ltd.), SOD kit (Nanjing Jiancheng Bioengineering Institute), MDA kit (Nanjing Jiancheng Bioengineering Institute).

[0137] 2.3 Experimental methods

[0138] 110 male ICR mice, weighing 20-25 g, were randomly divided into 11 groups, 10 mice in each group, and the groups were respectively: blank control group, model group, positive drug group (Jisheng Shenqi Pills), experimental group (Example 1-3 groups and Comparative Example 1-5 groups); except the blank control group, the remaining mice were subcutaneously injected with hydrocortisone (20 mg / kg) for 10 consecutive days, and intramuscular injection (20 mg / kg) was changed on the 11th and 12th days, and the mice were free to eat and drink water. After the modeling was completed, the changes in the diet, body weight, fur gloss, mental state, autonomous activity, etc. of the mice were observed to determine whether the model was successful; while modeling, each experimental group was gavaged with the corresponding composition (the total crude drug amount was 5 g / kg), and the positive drug group was gavaged with Jisheng Shenqi Pills (crude drug amount 3 g / kg) for 12 consecutive days.

[0139] 2.4 Index detection

[0140] (1) On the 11th day of the experiment, 1 hour after administration of the combination or Jisheng Shenqi Pills, 0.2 mL of blood was collected from the posterior ocular venous plexus of each group of mice, the blood was kept in a water bath for 30 min, and centrifuged at 3500 rpm for 10 min. The SOD and MDA levels in serum were determined according to the instructions of the mouse SOD and MDA kits. The xanthine oxidase method was used for the detection of SOD, and the thiobarbituric acid (TBA) colorimetric analysis method was used for the detection of MDA.

[0141] (2) On the 12th day of the experiment, 1 hour after administration of the composition or Jisheng Shenqi Pills, the following indicators were observed: ① Body weight, the body weight of each group of mice before and after administration of the composition or Jisheng Shenqi Pills was weighed, and the weight growth rate was calculated as follows = (weight after administration of the composition / Jisheng Shenqi Pills - weight before administration of the composition / Jisheng Shenqi Pills) / weight before administration of the composition / Jisheng Shenqi Pills; ② Low-temperature swimming time, the mice were placed in a square box with a length of 50 cm, a width of 40 cm, and a water depth of 20 cm. The water temperature was controlled at 10±1°C by ice packs, and the swimming survival time from entering the water to drowning was recorded. ③ Organ index, after recording the swimming survival time of the mice, the mice were immediately removed for dissection, and the thymus, bilateral kidneys and testicles (including epididymis) were removed, and the wet weight was weighed to calculate the organ index, which was organ weight / body weight. The removed organs were placed in a 10% formaldehyde solution for preservation.

[0142] 2.5 Test Results

[0143] 2.5.1 Contents of SOD and MDA in serum

[0144] As shown in Table 2:

[0145] Table 2 Contents of SOD and MDA in serum

[0146]

[0147]

[0148] As can be seen from Table 2, compared with the blank control group, the SOD activity in the serum was significantly decreased, and the MDA content was significantly increased, indicating that the model was successfully established. Compared with the model group, the SOD activity in the serum of the mice in Example Groups 1-3 and Comparative Example Groups 1-6 was significantly increased, and the serum MDA content was significantly decreased, especially in Example Group 3. By comparing Example Group 3 with Comparative Example 1 and 2, it can be seen that the fruit oil of Cornus wilsoniana and the fruit oil of Rhus chinensis can increase the SOD activity in the serum, decrease the MDA content, increase the content of oxygen free radicals in the body, improve the antioxidant capacity in the body, and accelerate the metabolism in the body. By comparing Example 3 with Comparative Example 3, it can be seen that using an ethanol aqueous solution as an entrainer has no effect on the SOD content in the body compared with using peony seed oil as an entrainer, but using an ethanol aqueous solution as an entrainer will increase the MDA content, resulting in membrane lipid peroxidation damage to the biological membrane structure, damaging the cell membrane structure and function, changing the membrane permeability, and thus affecting the normal progress of a series of physiological and biochemical reactions. By comparing Example 3 with Comparative Example 4 and 5, it can be seen that the present technical solution has a certain selectivity for Melastoma dodecandrum Lour. extract and complex enzyme, and only by using specific extraction methods and enzyme types can better effects be achieved.

[0149] 2.5.2 Changes in body weight, low-temperature swimming time and organ index

[0150] As shown in Table 3:

[0151] Table 3 Changes in body weight, low-temperature swimming time and organ index

[0152]

[0153]

[0154] As can be seen from Table 3, the effect of Example 3 is the best. From the perspective of the body weight growth rate, compared with the blank control group and the model group, the body weight increased significantly, indicating that the establishment of the yang-deficiency mouse model was successful. Compared with the model group, the body weights of the mice in the positive drug group and the experimental groups increased significantly, and the increase in the body weight of the mice in Example Group 3 was the most obvious; from the perspective of the low-temperature swimming time, compared with the model group, the low-temperature swimming times of the mice in the positive drug group and the experimental groups increased; from the perspective of the thymus, bilateral kidney and bilateral testis + epididymis indices, the thymus indices of the mice in the model group were significantly reduced compared with the blank control group, indicating that the model was successfully established. Compared with the model group, the thymus indices, bilateral kidney indices and testis indices of the mice in the positive drug group and the experimental groups increased; the above results show that the composition of the present invention has the effect of warming yang and tonifying the kidney, replenishing the yang qi in the kidney to achieve the purpose of regulating the functions of the zang-fu organs and enhancing the physique. Only by completely adding the raw materials according to this formula can the best kidney-tonifying effect be achieved.

[0155] Experimental Example 3 Improving immunity

[0156] 3.1 Experimental materials

[0157] The composition prepared in Example 3 was replaced by distilled water as a control group, and 20 mg / kg was administered intragastrically;

[0158] 3.2 Experimental methods

[0159] Fifty healthy secondary female ICR mice weighing 20-25 g were selected and divided into two groups, with 25 mice in each group. The number of antibody-producing cells and the half hemolytic value (HC 50 ) determination; the experimental mice were fed with a special feed for outbred mice, and various indicators were measured after gavage once a day for 30 consecutive days.

[0160] 3.2.1 Determination of the number of antibody-producing cells (Jerne modified slide method)

[0161] Take sheep blood, wash with saline for 3 times, and inject 2% (v / v, prepared with saline) SRBC 0.2mL into each mouse through the peritoneal cavity. Mice immunized with SRBC for 5 days were killed, spleens were taken, and cell suspensions were prepared by grinding. After the agarose was heated and dissolved, it was mixed with an equal amount of double Hanks solution and dispensed into small test tubes, 0.5mL per tube. Then 10% (v / v, prepared with SA solution) SRBC 50μL and spleen cell suspension 20μL were added to the tubes, mixed quickly, and poured onto the glass slide brushed with a thin layer of agarose. After the agar solidified, the glass slide was placed horizontally on the slide rack and placed in a carbon dioxide incubator for incubation for 1.5h, and then complement (1:10) diluted with SA buffer was added to the groove of the slide rack. After continuing to incubate for 1.5h, the number of hemolytic plaques was counted.

[0162] The data obtained are quantitative data. If the number of hemolytic plaques in the combination group is significantly higher than that in the control group, and the difference is significant (P < 0.05), it can be determined that the combination has the effect of increasing the number of antibody-producing cells in mice.

[0163] 3.2.2 Half hemolysis value (HC 50 )

[0164] Take sheep blood, wash with saline for 3 times, and inject 2% (v / v, prepared with saline) SRBC 0.2mL into each mouse for immunization. 5 days later, remove the eyeball and take blood into a centrifuge tube, place it for about 1 hour, peel off the coagulated blood from the tube wall, let the serum fully precipitate, centrifuge at 2000rpm for 10min, and collect the serum. Dilute the serum 400 times with SA buffer, take 1mL and put it into a test tube, add 10% (v / v, prepared with SA buffer) SRBC 0.5mL, complement 1mL (diluted 1:10 with SA buffer) in turn. Set up a control tube without serum (replaced with SA buffer). After keeping warm in 37℃ constant temperature water for 30min, stop the reaction in ice bath. Centrifuge at 2000rpm for 10min, take 1mL of supernatant, and add 3mL of Du's reagent. At the same time, take 0.25 mL of 10% (v / v, prepared with SA buffer) compressed SRBC, add Dulbecco's reagent to 4 mL in another test tube, mix thoroughly, and place for 10 minutes. Then, use the control tube as a blank and measure the optical density of each tube at 540 nm. The amount of hemolysin is measured by the half hemolysis value (HC 50 ) is expressed as follows:

[0165] Sample half hemolysis value = sample optical density value / SRBC half hemolysis optical density value × dilution multiple;

[0166] The data obtained are quantitative data. If the half-value of hemolysis of the combination group is significantly higher than that of the control group, and the difference is significant (P < 0.05), it can be determined that the combination has the effect of increasing the half-value of hemolysis of mice.

[0167] 3.3 Test Results

[0168] The experimental data were tested for variance homogeneity using Stata software. The data that met the "homogeneity of variance" requirement were statistically processed using one-way analysis of variance and pairwise comparison of means between multiple experimental groups and a control group. Appropriate variable transformation was performed on data with non-normal distribution or uneven variance, and the transformed data were processed after meeting the "normal homogeneity of variance" requirement.

[0169] 3.3.1 Effects of the composition on the number of antibody-producing cells in mice, as shown in Table 4:

[0170] Table 4 Effect of the composition on the number of mouse antibody-producing cells

[0171] Group <![CDATA[Number of hemolytic plaques (×10 3 / total spleen)]]> P-value Example 3 Group <![CDATA[201±53 * ]]> 0.021 Control group 162±45 —

[0172] 3.3.2 Effects of the composition on the half hemolysis value of mice are shown in Table 5:

[0173] Table 5 Effect of the composition on the half hemolysis value of mice

[0174] Group Mouse half hemolysis value P-value Example 3 Group <![CDATA[315±72 * ]]> 0.013 Control group 372±81 —

[0175] As shown in Tables 4 and 5, there are significant differences between the Example 3 group and the control group (P < 0.05). The composition prepared in Example 3 can increase the number of antibody-producing cells in mice and increase the half-hemolytic value of mice, indicating that the composition has the function of enhancing immunity.

[0176] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition for warming and tonifying kidney yang and enhancing immunity, characterized in that: The invention comprises the following raw materials in parts by weight: 10-20 parts of elmaria fruit oil, 10-18 parts of rhus chinensis fruit oil, 8-15 parts of angustifolia extract, 7-12 parts of melaleuca extract, 6-11 parts of green tea, 6-10 parts of cinnamon bark, 4-8 parts of wolfberry, 4-8 parts of raspberry, 3-7 parts of mulberry, 3-6 parts of astragalus, 2-5 parts of polygonatum, 2-4 parts of epimedium, 1-4 parts of cistanche, 1-5 parts of morinda officinalis, and 1-3 parts of isomalt.

2. A composition for warming and tonifying kidney yang and enhancing immunity according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15 parts of elm wood oil, 15 parts of rhus chinensis oil, 12 parts of angustifolia extract, 10 parts of chelidonium extract, 9 parts of green tea, 9 parts of cinnamon bark, 7 parts of wolfberry, 6 parts of raspberry, 5 parts of mulberry, 5 parts of astragalus, 4 parts of polygonatum, 3 parts of epimedium, 3 parts of cistanche, 3 parts of morinda officinalis, and 2 parts of isomalt.

3. A composition for warming and tonifying kidney yang and enhancing immunity according to claim 1, characterized in that: The preparation method of an epilobium angustifolium extract comprises the following steps: drying and crushing the epilobium angustifolium, mixing it with ethyl acetate, and performing ultrasonic pretreatment; extracting the pretreated material with supercritical carbon dioxide fluid and peony seed oil at a temperature of 40-50 DEG C and a pressure of 20-25Mpa for 100-120 minutes to obtain an extract; and decompressing and concentrating the extract to a relative density of 1.35-1.40 to obtain an epilobium angustifolium extract.

4. A composition for warming and tonifying kidney yang and enhancing immunity according to claim 3, characterized in that: The mass ratio of angustifolia to ethyl acetate is 1:(8-12), and the weight ratio of the pretreated material to peony seed oil is 1:(0.4-0.9).

5. A composition for warming and tonifying kidney yang and enhancing immunity according to claim 3, characterized in that: In the ultrasonic pretreatment, the ultrasonic power is 280-450W, and the ultrasonic treatment time is 20-60min.

6. A composition for warming and tonifying kidney yang and enhancing immunity according to claim 3, characterized in that: The flow rate of the supercritical carbon dioxide fluid is 15 to 25 L / hour.

7. A composition for warming and tonifying kidney yang and enhancing immunity according to claim 1, characterized in that: The preparation method of the radix spatholobi extract comprises the following steps: S1. Wash fresh radish and place it in water, grind the radish in the water, wherein the ratio of radish to water is 1:(20-25) by mass, and place the water container in an ice water bath to obtain radish grinding liquid; S2, adding 0.1-1 mol / L HCl solution to the ground radix schizonepetae to adjust the pH to 4.5-6.5, and then adding 0.1-1.5% of the total weight of α-amylase and 2-5% of saccharifying enzyme for enzymolysis for 4-7 hours to obtain an enzymolysis solution; S3, placing the enzymatic hydrolyzate obtained in step S2 in a 95-100°C water bath to inactivate the enzyme for 20-30 minutes; S4, placing the enzyme-killed hydrolyzate obtained in step S3 at a preset temperature of 0-4°C for refrigerated centrifugation, maintaining the speed at 5000-7000 r / min, collecting the upper clear liquid, filtering the clear liquid through a 0.3-0.6 μm filter element, and drying to obtain the radix schizonepetae extract.

8. The method for preparing a composition for warming and tonifying kidney yang and enhancing immunity according to claim 1, characterized in that: The following steps are involved: (1) Grinding: Dry and grind green tea, cinnamon bark, wolfberry, raspberry, mulberry, astragalus, polygonatum, epimedium, cistanche and Morinda officinalis, and separate and sieve through 100-200 mesh to obtain a mixed dry powder for later use; (2) Enzymatic hydrolysis: Adding the fruit oil of Elaeagnus glabra, the fruit oil of Rhus chinensis, the extract of Epilobium angustifolium, the extract of Elaeagnus oleifera and isomalt to the mixed dry powder and stirring evenly to obtain a mixed solution; adjusting the pH value of the mixed solution to 6.0-6.5, adding a composite enzyme, and performing enzymatic hydrolysis at a temperature of 35-45° C. for 4-7 hours to obtain an enzymatic hydrolyzate; (3) Inactivating the enzyme: placing the enzymatic solution obtained in step (2) at a temperature of 110 to 120° C. to inactivate the enzyme for 12 to 20 minutes; (4) Filtration: Centrifuge the enzyme-inactivated hydrolyzate at 3000-4500 rpm for 8-15 min and collect the supernatant; (5) Concentration: The supernatant is concentrated under reduced pressure to obtain a composition that warms the kidney yang and enhances immunity.

9. The method for preparing the composition for warming and tonifying kidney yang and enhancing immunity according to claim 8, characterized in that: The compound enzyme is a compound ratio of tanninase and xylanase in a mass ratio of 1:(2-4), and the added amount of the compound enzyme is 0.5-1.5% of the total mass of the mixed solution.

10. Use of the composition according to any one of claims 1 to 7 or the composition prepared by the preparation method according to claim 8 or 9 in the preparation of functional foods for warming and nourishing kidney yang and enhancing immunity.

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