Anti-hair loss shampoo containing leech extract and preparation method of anti-hair loss shampoo

Abstract

The invention discloses a leech extract anti-hair loss shampoo and a preparation method thereof, the raw material components are as follows: 72.4 parts of water, 12 parts of sodium laureth sulfate, 5 parts of cocamidopropyl betaine, 3 parts of ammonium lauryl sulfate, 5 parts of coco-glucoside and 1.6 parts of cocamidopropyl DEA are added into a batching kettle, stirred and heated to 80 DEG C, and stirred and dissolved completely. Stirring and cooling to below 40 DEG C, adding 0.1 part of hydroxypropyl trimethyl ammonium chloride hyaluronic acid, 0.6 part of phenoxyethanol, 0.1 part of essence, 0.1 part of EDTA-disodium and 0.1 part of leech extract, and stirring and dissolving completely; as a natural anticoagulant, hirudin provides sufficient oxygen and nutrition for hair follicles by inhibiting thrombin activity, promoting blood circulation and improving the microcirculation environment of scalp. Besides, the anti-inflammatory and anti-oxidation effects of the hirudin can relieve inflammatory reaction of scalp, protect hair follicle cells from being damaged by free radicals, delay the aging process of the hair follicle cells and protect hair follicles from being damaged by oxidative stress.

Description

Technical Field

[0001] The invention relates to the technical field of anti-hair loss shampoo, in particular to a leech extract anti-hair loss shampoo and a preparation method thereof. Background Art

[0002] Hair loss is one of the most concerned clinical diseases in the world, affecting people of all ages and genders. Female pattern hair loss (FPHL) is the most common type of hair loss in women. Its causes are diverse, mostly caused by the imbalance of hair follicle cycle under the influence of various factors such as environment, genetics, hormones, and aging. In recent years, FPHL has shown a trend of becoming younger. It not only directly affects the appearance of patients, but is also often accompanied by varying degrees of anxiety, depression and other emotions, affecting the mental health of patients. At present, there are many methods and products for the treatment of FPHL, but the effects often vary from person to person, and there are certain adverse reactions. Summary of the invention

[0003] The object of the present invention is to provide a leech extract anti-hair loss shampoo and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0004] To achieve the above object, the present invention provides the following technical solution: a leech extract anti-hair loss shampoo, characterized in that its preparation raw materials include the following components in parts by mass: 70-75 parts of water, 8-14 parts of anionic surfactants, 4-6 parts of zwitterionic surfactants, 2-4 parts of foaming agents, 4-6 parts of detergents, 1-2 parts of cocamide, 0.08-0.15 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.5-0.7 parts of phenoxyethanol, 0.08-0.15 parts of EDTA-disodium, 0.08-0.15 parts of leech extract, and 0.08-0.15 parts of flavor.

[0005] Furthermore, the anionic surfactant is sodium laureth sulfate, the zwitterionic surfactant is cocamidopropyl betaine, the foaming agent is ammonium lauryl sulfate, and the cleaning agent is coconut glucoside.

[0006] Furthermore, the raw material components are 72.4 parts of water, 12 parts of sodium laureth sulfate, 5 parts of cocamidopropyl betaine, 3 parts of ammonium lauryl sulfate, 5 parts of coco-glucoside, 1.6 parts of cocamide DEA, 0.1 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.6 parts of phenoxyethanol, 0.1 parts of essence, 0.1 parts of EDTA-disodium, and 0.1 parts of leech extract.

[0007] The preparation method of leech extract anti-hair loss shampoo comprises the following steps: Step 1, materials and reagents: prepare 0.1 parts of water, sodium laureth sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, cocoyl glucoside, cocamide, hydroxypropyltrimonium chloride hyaluronic acid, phenoxyethanol, essence, disodium EDTA, and leech extract; Step 2, ingredients: add water, sodium laureth sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, coco-glucoside and cocamide DEA into a mixing kettle, stir and heat to 75-85°C; cool to below 40°C, add hydroxypropyltrimonium chloride hyaluronic acid, phenoxyethanol, flavor, disodium EDTA and leech extract, and stir to dissolve completely.

[0008] Compared with the prior art, the present invention has the following beneficial effects: (1) Hirudin is an active ingredient in leech extract. As a natural anticoagulant, it inhibits thrombin activity, promotes blood circulation, and improves the microcirculation environment of the scalp, thereby providing sufficient oxygen and nutrients to the hair follicles. In addition, the anti-inflammatory and antioxidant effects of hirudin can reduce the inflammatory response of the scalp, protect hair follicle cells from damage by free radicals, delay the aging process of hair follicle cells, and protect hair follicles from damage by oxidative stress.

[0009] (2) Leech extract containing hirudin is a natural ingredient with few side effects. No obvious adverse reactions were found in clinical use of leech extract shampoo. Secondly, leech extract shampoo is easy to use and can be easily used at home, which is in line with the fast-paced lifestyle of modern people. In addition, based on the anti-inflammatory and antioxidant properties of hirudin, it not only has the effect of preventing hair loss, but also can improve scalp health and improve hair quality, meeting the comprehensive needs of patients. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 is a preparation flow chart of the present invention; Figure 2 This is a comparison chart of trichoscopic examination report data before and after the treatment of the present invention. DETAILED DESCRIPTION

[0011] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example

[0012] See also Figure 1-2 , the present invention provides a technical solution: a leech extract anti-hair loss shampoo and a preparation method; 1. Materials and Methods 1.1 Materials and reagents Sodium laureth sulfate (BASF (China) Co., Ltd.), cocamidopropyl betaine (Guangzhou Tianci High-tech Materials Co., Ltd.), ammonium lauryl sulfate (BASF (China) Co., Ltd.), coco-glucoside (BASF (China) Co., Ltd.), cocamide DEA (BASF (China) Co., Ltd.), hydroxypropyltrimonium chloride hyaluronic acid (Nanjing Jianweit Biotechnology Co., Ltd.), phenoxyethanol (Clariant Chemical Technology (Shanghai) Co., Ltd.), flavor (Guangzhou Chenyou Flavors & Fragrances Co., Ltd.), disodium EDTA (Nouryon Chemicals (Ningbo) Co., Ltd.), leech extract (Yunnan Meicai Biotechnology Co., Ltd.).

[0013] 1.2 Preparation of shampoo: Mix the following three types of shampoo in proportion by weight: (I) Add 70 parts of water, 8 parts of sodium laureth sulfate, 4 parts of cocamidopropyl betaine, 2 parts of ammonium lauryl sulfate, 4 parts of cocoyl glucoside, and 1 part of cocamide DEA to the mixing kettle, stir and heat to 80°C, and stir to dissolve completely. Stir and cool to below 40°C, add 0.08 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.5 parts of phenoxyethanol, parts of essence (0.1%), 0.08 parts of EDTA-disodium, and 0.08 parts of leech extract, and stir to dissolve completely.

[0014] (ii) 72.4 parts of water, 12 parts of sodium laureth sulfate, 5 parts of cocamidopropyl betaine, 3 parts of ammonium lauryl sulfate, 5 parts of coco-glucoside, 1.6 parts of cocamide DEA, 0.1 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.6 parts of phenoxyethanol, 0.1 parts of flavor, 0.1 parts of disodium EDTA, and 0.1 parts of leech extract.

[0015] (III) 75 parts of water, 14 parts of sodium laureth sulfate, 6 parts of cocamidopropyl betaine, 4 parts of ammonium lauryl sulfate, 6 parts of coco-glucoside, 2 parts of cocamide DEA, 0.15 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.7 parts of phenoxyethanol, 0.15 parts of flavor, 0.15 parts of disodium EDTA, and 0.12 parts of leech extract.

[0016] 1.3 Equipment Digital dermatoscope (FotoFinder bodystudio ATBM, Germany, Ford system); 1.4 Clinical research data 1.4.1 Research subjects (1) Inclusion criteria: Referring to the diagnostic criteria of Clinical Dermatology, a total of 56 patients with female pattern hair loss were included. (2) Exclusion criteria: patients with hair loss caused by drugs, systemic diseases, immune diseases, fungal infections, congenital hair loss, etc.; patients who are pregnant or breastfeeding.

[0017] 1.4.2 Research Methods Hirudin anti-hair loss shampoo was uniformly distributed for 10 weeks of treatment and observation. According to the patient's condition and usage habits, the hair was washed once every 1 to 2 days. The general information of the included patients was collected, and the clinical symptoms were monitored at the 0th, 2nd, 4th, 6th, 8th, and 10th weeks of treatment.

[0018] 1.4.3 Observation indicators Every 2 weeks, doctors observed and recorded the patient's hair loss condition, subjective score sheet, Female Pattern Hair Loss Severity Scale (FPHLSL), dermoscopic score sheet, and Dermatology Life Quality Index (DLQI) score sheet. The dermoscopic hair system examination of the patient was completed by the Dermatology Specialty Hospital of Yunnan Provincial Hospital of Traditional Chinese Medicine; Female pattern hair loss (FPHL), also known as female androgenic alopecia, is the most common form of non-scarring hair loss in women. It is a slowly progressive disease that often occurs after puberty, and is mainly manifested by miniaturization of hair follicles and progressive hair loss. Specifically, it can be evaluated by observing the progressive diffuse decrease in hair density on the top and front of the head. In addition, the Ludwig classification method can also be used to grade the degree of hair loss; The Ludwig classification is a method for assessing the severity of female pattern hair loss, which is divided into the following three levels: Mild hair loss (Grade I): The hair in the crown area of ​​the head begins to thin, but a 1-3 cm wide forehead hairline is still retained.

[0019] Moderate hair loss (Grade II): Hair thinning increases in the same area, and the scalp is noticeably exposed.

[0020] Severe hair loss (Grade III): In the hair loss area of ​​Grades I and II, the hair falls out completely and the scalp is completely exposed.

[0021] 1.4.4 Efficacy criteria The efficacy evaluation standard was formulated with reference to the Guiding Principles for Clinical Research of New Chinese Medicines and based on the Nimodipine method: subjective score reduction index (%) = [(score before treatment - score after treatment) / score before treatment] x 100%.

[0022] 1.4.5 Statistical methods Data processing was performed using SPSS 26.0 statistical software for statistical analysis. The measurement data that conformed to normal distribution were tested with t test and described with (mean ± standard deviation); the count data were expressed as cases (%), and the chi-square test was used for normal distribution, and the rank sum test was used for those that did not conform to normal distribution. The test level was set at a = 0.05, and P < 0.05 was considered statistically significant.

[0023] 2. Treatment outcomes 2.1 General Information The average age of the patients was (30.52±5.59) years old, age range: 20-40 years old, median height 160cm, height range: 150-178, average weight (56.52±9.00)kg, weight range: 41-80kg, average BMI (21.64±2.97), BMI range: 16.22-31.25, most of the patients had a bachelor's degree, most of the patients had a normal body shape, the duration of hair loss was mainly 1 to 5 years, and the majority of patients had the habit of staying up late.

[0024] Table 1 General information

[0025] 2.2 Comparison of various observation indicators before and after treatment After treatment, the patients' subjective scores, FPHLSL scores, and Dermatology Quality of Life Scale scores all showed a gradual downward trend. The scores of various observation indicators were compared within the group with the previous treatment time point, and there were significant statistical differences (P < 0.01), indicating that the treatment had a good clinical effect.

[0026] Table 2 Comparison of various observation indicators before and after treatment (X±s, points) Before treatment 2 weeks of treatment 4 weeks of treatment Treatment for 6 weeks 8 weeks of treatment 10 weeks of treatment Subjective Situation Rating 15.59±5.73 <![CDATA[14.16±5.31 a ]]> <![CDATA[12.95±5.26 b ]]> <![CDATA[11.36±4.78 c ]]> <![CDATA[9.75±4.9 d ]]> <![CDATA[8.57±4.8 e <!-- 4 -->]]> FPHL-SL score 8.84±3.29 <![CDATA[8.46±3.26 a ]]> <![CDATA[8.13±3.33 b ]]> <![CDATA[7.63±3.41 b ]]> <![CDATA[6.95±3.53 d ]]> <![CDATA[6.64±3.61 e ]]> Dermatology life quality scale score 5.46±5.1 <![CDATA[4.64±4.49 a ]]> <![CDATA[4.09±4.06 b ]]> <![CDATA[3.16±3.4 b ]]> <![CDATA[2.5±2.85 d ]]> <![CDATA[1.89±2.27 e ]]> Note: Within the same group, compared with before treatment, aP<0.01; compared with 2 weeks of treatment, bP<0.01; compared with 4 weeks of treatment, cP<0.01; compared with 6 weeks of treatment, dP<0.01; compared with 8 weeks of treatment, eP<0.01.

[0027] 2.3 Subjective rating of efficacy The efficacy was evaluated by subjective scores before and after treatment, and the effective rate showed a gradual upward trend. The effective rate was 5.36% after 2 weeks of treatment and increased to 71.43% after 10 weeks of treatment, with a significant effect.

[0028] Table 4 Subjective scoring of efficacy 2 weeks of treatment 4 weeks of treatment Treatment for 6 weeks 8 weeks of treatment 10 weeks of treatment invalid 53（94.64） 42（75.00） 33（58.93） 20（35.71） 16（28.57） efficient 3（5.36） 14（25.00） 23（41.07） 32（57.14） 29（51.79） Significant effect 0 0 0 4 11 Effectiveness (%) 5.36 25.00 41.07 64.29 71.43 2.4 Comparison of various observation indicators of trichoscope before and after treatment After treatment, the number of hairs per cm², the average hair diameter, and the proportion of hairs per follicle (4 hairs) showed a gradual upward trend. The levels of the above observation indicators were compared with the levels at the previous treatment time point, and the differences were statistically significant (P < 0.05). The proportion of hairs per follicle (1 hair, 2 hairs) and the proportion of vellus hair showed a downward trend. The differences were statistically significant when compared with the levels at the previous treatment time point (P < 0.05). The proportion of hairs per follicle (3 hairs) showed a statistically significant difference between before and after treatment after 4 weeks of treatment (P < 0.05).

[0029] Table 3 Comparison of various observation indicators under the hair microscope before and after treatment (X±s) Before treatment 4 weeks of treatment 8 weeks of treatment 10 weeks of treatment Number of hairs per 1cm² 128.33±34.16 <![CDATA[128.5±34.16 a ]]> <![CDATA[129.13±34.09 c ]]> <![CDATA[129.72±34.24 e ]]> Average hair diameter 53.6±11.33 <![CDATA[53.62±11.33 a ]]> <![CDATA[53.85±11.3 c ]]> <![CDATA[53.86±11.29 e ]]> Number of hair per follicle (1 hair, %) 47.49±17.99 <![CDATA[47.41±17.98 a ]]> <![CDATA[47.13±17.99 c ]]> <![CDATA[47.06±18.03 e ]]> Number of hair per follicle (2, %) 41.89±15.93 <![CDATA[41.78±15.96 a ]]> <![CDATA[41.59±15.94 c ]]> <![CDATA[41.51±15.97 e ]]> Number of hair per follicle (3, %) 9.19±9.49 <![CDATA[9.12±9.43 a ]]> 9.10±9.4 9.11±9.37 Number of hair per follicle (4, %) 1.43±4.42 <![CDATA[1.71±4.4 a ]]> <![CDATA[2.2±4.38 c ]]> <![CDATA[2.34±4.52 e ]]> Vellus hair ratio (%) 22.99±16.96 <![CDATA[22.79±16.82 a ]]> <![CDATA[22.53±16.77 c ]]> <![CDATA[22.09±16.6 e ]]> Note: within the same group, compared with before treatment, aP<0.05; compared with 4 weeks of treatment, cP<0.05; compared with 8 weeks of treatment, eP<0.05.

[0030] 2.5 FPHL-SL Scoring Criteria After treatment, the rate of non-female hair loss assessed by the FPHL-SL scoring standard showed a gradual upward trend, from 1.79% before treatment to 30.36% after 10 weeks of treatment, with significant results.

[0031] Table 5 FPHLSL scoring criteria Before treatment 2 weeks of treatment 4 weeks of treatment Treatment for 6 weeks 8 weeks of treatment 10 weeks of treatment Non-female hair loss 1（1.79） 3（5.36） 4（7.14） 10（17.86） 14（25.00） 17（30.36） Early 32（57.14） 31（55.36） 31（55.36） 27（48.21） 23（41.07） 23（41.07） Confirmation 23（41.07） 22（39.29） 21（37.50） 19（33.93） 19（33.93） 16（28.57） 2.6 Impact on quality of life After treatment, the impact on patients' quality of life gradually eased, and the proportion of unaffected patients increased from 25.00% to 55.36%, and patients' quality of life was significantly improved.

[0032] Table 6 Impact on quality of life Before treatment 2 weeks of treatment 4 weeks of treatment Treatment for 6 weeks 8 weeks of treatment 10 weeks of treatment No impact 14（25.00） 15（26.79） 18（32.14） 26（46.43） 27（48.21） 31（55.36） Mild 22（39.29） 22（39.29） 20（35.71） 16（28.57） 19（33.93） 21（37.50） Moderate 10（17.86） 13（23.21） 13（23.21） 11（19.64） 9（16.07） 4（7.14） Severe 10（17.86） 6（10.71） 5（8.93） 3（5.36） 1（1.79） 0 3. Discussion The average age of the patients included in this example was 30.52 years old, and the age range was 20 to 40 years old, indicating that female pattern hair loss has a higher incidence in young and middle-aged women. The average weight of the patients was 56.52 kg, and the average BMI was 21.64, indicating that most patients were of normal body shape. The duration of hair loss in the subjects was mainly concentrated in 1 to 5 years, and most patients had the habit of staying up late, suggesting that modern lifestyle and stress have a more significant impact on female pattern hair loss.

[0033] The results of this embodiment show that the subjective total score, FPHLSL score and dermatological quality of life scale score of the patients after treatment all show a significant downward trend, which indicates that the leech extract anti-hair loss shampoo has a significant effect in improving hair loss symptoms, promoting hair growth and improving the quality of life of patients. Among them, the significant decrease in the subjective total score indicates that the patient's self-perception is significantly improved after using the leech extract shampoo, and the subjective feelings such as reduced hair loss, improved hair quality, and optimized scalp condition have been improved, which proves the effectiveness of leech shampoo in actual use. The hair mirror data at the 0th, 4th, 8th and 10th weeks of treatment showed that the number of hair per square centimeter, the average diameter of hair and the proportion of 4 hairs in a unit hair follicle increased significantly after treatment, indicating that the leech extract shampoo has a positive effect in increasing hair density and improving hair quality (P<0.05). At the same time, the proportion of 1 or 2 hairs in a unit hair follicle and the proportion of vellus hair decreased significantly, indicating that the health of the hair follicles has improved and more hair follicles have entered the active growth phase (P<0.05). The proportion of 3 hairs in a hair follicle also changed significantly after 4 weeks of treatment compared with before treatment (P<0.05), further supporting the effectiveness of hirudin. These results show that leech extract shampoo can significantly promote hair regeneration and improve hair quality, and enhance patients' self-confidence and mental health.

[0034] Hirudin is an active ingredient in leech extract. As a natural anticoagulant, it inhibits thrombin activity, promotes blood circulation, and may improve the microcirculation environment of the scalp, thereby providing sufficient oxygen and nutrition for hair follicles. In addition, the anti-inflammatory and antioxidant effects of hirudin can reduce the inflammatory response of the scalp, protect hair follicle cells from damage by free radicals, delay the aging process of hair follicle cells, and protect hair follicles from damage by oxidative stress. At present, common hair loss treatments on the market include minoxidil, finasteride and other drug treatments, hair transplant surgery, and various anti-hair loss products. Compared with these traditional methods, Chinese herbal ingredients in anti-hair loss shampoo has a more prominent advantage in clinical applications; leech extract containing hirudin is a natural ingredient with fewer side effects. Compared with the potential side effects of drug treatment, such as scalp irritation of minoxidil and sexual dysfunction of finasteride, no obvious adverse reactions were found in the clinical use of leech extract shampoo. Secondly, leech extract shampoo is easy to use and patients can use it easily at home, which is in line with the fast-paced lifestyle of modern people. In addition, based on the anti-inflammatory and antioxidant properties of hirudin, it not only has the effect of preventing hair loss, but also can improve scalp health and improve hair quality. It has multiple functions and meets the comprehensive needs of patients.

[0035] In summary, leech extract anti-hair loss shampoo has significant clinical effects in treating female pattern hair loss and promoting hair growth, and it is highly safe and easy to use.

[0036] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A leech extract anti-hair loss shampoo, characterized in that: The raw materials for its preparation include the following components in parts by weight: 70-75 parts of water, 8-14 parts of anionic surfactants, 4-6 parts of zwitterionic surfactants, 2-4 parts of foaming agents, 4-6 parts of detergents, 1-2 parts of cocamide, 0.08-0.15 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.5-0.7 parts of phenoxyethanol, 0.08-0.15 parts of EDTA-disodium, and 0.08-0.12 parts of leech extract.

2. The leech extract anti-hair loss shampoo according to claim 1, characterized in that: It also includes 0.08-0.15 parts of flavor.

3. The leech extract anti-hair loss shampoo according to claim 2, characterized in that: The anionic surfactant is sodium laureth sulfate.

4. The leech extract anti-hair loss shampoo according to claim 3, characterized in that: The zwitterionic surfactant is cocamidopropyl betaine.

5. The leech extract anti-hair loss shampoo according to claim 4, characterized in that: The foaming agent is ammonium lauryl sulfate.

6. The leech extract anti-hair loss shampoo according to claim 5, characterized in that: The cleaning agent is coco-glucoside.

7. The leech extract anti-hair loss shampoo according to claim 6, characterized in that: The raw material components are 72.4 parts of water, 12 parts of sodium laureth sulfate, 5 parts of cocamidopropyl betaine, 3 parts of ammonium lauryl sulfate, 5 parts of coco-glucoside, 1.6 parts of cocamide DEA, 0.1 parts of hydroxypropyltrimonium chloride hyaluronic acid, 0.6 parts of phenoxyethanol, 0.1 parts of essence, 0.1 parts of disodium EDTA, and 0.1 parts of leech extract.

8. A method for preparing the leech extract anti-hair loss shampoo according to any one of claims 1 to 7, characterized in that: The following steps are involved: Step 1, materials and reagents: prepare water, sodium laureth sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, cocoyl glucoside, cocamide, hydroxypropyltrimonium chloride hyaluronic acid, phenoxyethanol, essence, disodium EDTA, leech extract; Step 2, ingredients: add water, sodium laureth sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, coco-glucoside and cocamide DEA into a mixing kettle, stir and heat; cool to below 40°C, add hydroxypropyltrimonium chloride hyaluronic acid, phenoxyethanol, flavor, disodium EDTA and leech extract, and stir to dissolve completely.

9. The method for preparing a leech extract anti-hair loss shampoo according to claim 8, characterized in that: The mixing kettle is stirred and heated at a temperature of 75-85°C.

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