Deep-sea multi-sequence in-situ seawater filtering and treating device

By designing a deep-sea multi-sequence in situ seawater filtration and treatment device, and switching sampling channels with multi-channel valves, the problem of low sample quality in deep-sea sampling technology is solved, and efficient sample acquisition and storage in deep-sea environment is achieved, providing sufficient The high-quality samples of the quantity provide important support for the development of deep-sea resources and scientific research.

CN119160958APending Publication Date: 2024-12-20SHENZHEN LANHAI RONGSHENG MARINE TECHNOLOGY DEVELOPMENT CO LTD

Patent Information

Application Number
CN202411115015.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-08-14
Publication Date
2024-12-20

AI Technical Summary

Technical Problem

The existing deep-sea sampling technology leads to low sample quality under temperature and pressure changes, and it is impossible to effectively obtain high-quality deep-sea in situ microbial samples, which limits the development of deep-sea resources and scientific research.

Method used

A deep-sea multi-sequence in-situ seawater filtration and treatment device is designed, including pumps, filters, fixed liquid sample bags, two-position three-way valves, flowmeters, integrated chambers, pressure sensors, underwater control and energy supply units, multi- The channel valve and filter membrane cavity use multi-channel valves to switch sampling channels to achieve rapid and efficient enrichment of multi-time series microorganisms and particulate samples in situ in the deep sea.

Benefits of technology

The device can stably obtain samples of multiple time and stations in a deep-sea environment, avoiding the damage to the samples by environmental changes, ensuring the authenticity of gene information and the in-situ storage of samples, and providing sufficient high-quality samples , providing important support for the development of deep-sea resources and scientific research.

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Abstract

The invention discloses a deep-sea multi-sequence in-situ seawater filtering and processing device which comprises a pump, a filter, a stationary liquid sample bag, a two-position three-way valve, a flow meter, an integrated cavity integrating the two-position three-way valve and the flow meter, a pressure sensor, a pump for collecting data of the flow meter and the pressure sensor and a multi-channel valve, the underwater control and energy supply unit is used for controlling and supplying power by a two-position three-way valve; the output port of the two-position three-way valve is connected with the input port of the flowmeter, the normally open inlet is connected with the seawater inlet through the filter, the normally closed inlet is connected with the stationary liquid sample bag, and the inlet of the pump is connected with the output port of the flowmeter; an input channel is arranged between the pump and the multi-channel valve, two ends of the input channel are respectively connected with an input port of the multi-channel valve and an output port of the pump, and a pressure sensor is arranged on the input channel; a plurality of switchable working channels are arranged between the input port and the output ports of the multi-channel valve, the output ports are communicated with the filter membrane cavities through the filter channels, and the filter membrane cavities are connected with the seawater outlet.
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Description

Technical Field

[0001] The invention relates to deep sea sampling and in-situ preservation, and in particular to a deep sea multi-sequence in-situ seawater filtering and processing device. Background Art

[0002] Deep sea water contains a large number of natural products and microbial resources. Deep-sea microorganisms have an impact on the global ecosystem that cannot be ignored. They have evolved unique survival mechanisms in the extreme environment of the deep sea, including genes and metabolites with special functions. Although high-throughput sequencing technology has provided us with a large amount of genomic data, the low quality of deep-sea samples limits the development of these valuable resources. In addition, the rarity of marine microorganisms also poses a challenge to the study of metagenomics and metatranscriptomes, because these studies require a large number of high-quality deep-sea in situ microbial samples.

[0003] The traditional sampling technology is: the Nice Golden Bottle collects seawater and then filters it on board. This method is time-consuming and labor-intensive, and the collection process is affected by temperature and pressure changes, causing the sample to lose gene expression information. Therefore, it is necessary to develop and apply deep-sea in-situ high-throughput filtration, automated multi-sequence operations, and in-situ sample fixation processing sampling equipment to provide sufficient effective samples for research. Although relevant equipment has been developed both at home and abroad, the existing equipment can only collect one set of samples at a time, and to obtain a sufficient number of samples, the only way is to increase the number of dives. Therefore, it is hoped that multiple samples can be obtained in one dive, which is crucial for deep-sea resource acquisition and scientific research.

[0004] It should be noted that the information disclosed in the above background technology section is only used for understanding the background of the present application, and therefore may include information that does not constitute prior art known to ordinary technicians in the field. Summary of the invention

[0005] The invention provides a deep-sea multi-sequence in-situ seawater filtering and processing device.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme:

[0007] A deep-sea multi-sequence in-situ seawater filtration and treatment device, comprising a pump, a filter, a fixed liquid sample bag, a two-position three-way valve, a flowmeter, an integrated chamber, a pressure sensor, an underwater control and energy supply unit, a multi-channel valve and a filter membrane chamber, wherein: the two-position three-way valve and the flowmeter are integrated and installed in the integrated chamber; the output port of the two-position three-way valve is connected to the input port of the flowmeter, and the inlet of the pump is connected to the output port of the flowmeter; an input channel is provided between the pump and the multi-channel valve, one end of the input channel is communicated with the input port of the multi-channel valve, and the other end of the input channel is communicated with the output port of the pump, and a pressure sensor is connected to the passage of the input channel; the multi-channel valve has multiple output ports, and the input port of the multi-channel valve is connected to the input port of the multi-channel valve. A plurality of working channels are formed between one ends of the plurality of output ports of the multi-channel valve, and the multi-channel valve is controlled to rotate to switch each working channel, and the other end of each output port is connected to the inlet of the corresponding filter membrane cavity through a filter channel, and each filter membrane cavity is connected to the seawater outlet; the normally open inlet of the two-position three-way valve is connected to the seawater inlet through a filter, and the normally closed inlet of the two-position three-way valve is connected to the fixed liquid sample bag; the pump, the two-position three-way valve, the flow meter, the pressure sensor, and the multi-channel valve are respectively connected to the underwater control and power supply unit, and the underwater control and power supply unit is used to collect data from the flow meter and the pressure sensor, and to control and power the pump, the multi-channel valve, and the two-position three-way valve, respectively.

[0008] Preferably, a three-way valve is further included, and the pressure sensor is connected to the passage of the input channel through the three-way valve.

[0009] Preferably, the two-position three-way valve, the flow meter, the pump, the pressure sensor, and the multi-channel valve all adopt an oil compensation mode and are independently packaged.

[0010] Preferably, the filter membrane cavity comprises a plurality of separate filter membrane devices.

[0011] Preferably, it further comprises a first one-way valve and a second one-way valve, the normally closed inlet of the two-position three-way valve is connected to the fixed liquid sample bag through the first one-way valve, and each filter membrane cavity is connected to the seawater outlet through the second one-way valve.

[0012] Preferably, the integrated cavity is filled with oil, the two-position three-way valve and the flowmeter are respectively installed in the lower part and the upper part of the integrated cavity, the output port of the two-position three-way valve, the input port of the flowmeter, the output port of the flowmeter and the normally closed inlet of the two-position three-way valve are all located on the side of the integrated cavity, and the output port of the two-position three-way valve and the input port of the flowmeter are directly connected by a pipeline.

[0013] Preferably, the material of the cavity shell of the integrated cavity is POM plastic, the cavity of the integrated cavity is defined and sealed from the external liquid by an oil sac or an oil film, and the oil sac or the oil film and the oil filled in the integrated cavity provide pressure compensation for the cavity shell of the integrated cavity; an oil filling port is provided at the top of the integrated cavity.

[0014] Preferably, the multi-channel valve comprises a multi-channel valve head and a multi-channel valve oil filling chamber which are interconnected, the multi-channel valve having one multi-channel valve input port, one multi-channel valve communication port and a plurality of output ports; the plurality of output ports of the multi-channel valve are evenly distributed in the circumferential direction of the multi-channel valve head, one of the plurality of output ports is a multi-channel valve cleaning port, and the rest are working output ports of the multi-channel valve, one of the plurality of working channels formed between the input port of the multi-channel valve and one end of the plurality of output ports of the multi-channel valve is a cleaning channel, and the rest are sampling channels, the multi-channel valve oil filling chamber is provided with an underwater driving motor to drive the multi-channel valve to rotate, so as to convert the cleaning channel and each sampling channel on the multi-channel valve.

[0015] The present invention has beneficial effects: the present invention has a small and compact structure, high integration, strong corrosion resistance, supports long-term sampling, can quickly and effectively enrich multi-time series of microbial and particulate samples in deep sea in situ, and can be widely used for deep sea sample collection at different depths. By using a multi-channel valve to switch each sampling channel, the present invention can obtain samples at multiple times and positions in one dive, providing sufficient samples for subsequent research, which has great practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic structural diagram of a deep-sea multi-sequence in-situ seawater filtration and treatment device according to an embodiment of the present invention;

[0017] Figure 2 Schematic diagram of the structure of the integrated chamber 4 in the deep-sea multi-sequence in-situ seawater filtration and treatment device according to the embodiment of the present invention;

[0018] Figure 3 It is a schematic diagram of the structure of a multi-channel valve in a deep-sea multi-sequence in-situ seawater filtration and treatment device according to an embodiment of the present invention.

[0019] The following are the descriptions of the reference numerals:

[0020] 1-filter, 2-fixative liquid sample bag, 3-first one-way valve, 4-integrated chamber, 5-pump, 6-three-way valve, 7-pressure sensor, 8-multi-channel valve, 9-multi-channel valve head, 10-filtration channel, 11-filter membrane chamber, 12-second one-way valve, 13-input port of multi-channel valve, 14-input channel, 15-output port of flow meter, 16-normally closed inlet of two-position three-way valve, 17-normally open inlet of two-position three-way valve, 18-output port of two-position three-way valve, 19-input port of flow meter, 20-oil sac or oil film, 21-chamber housing of integrated chamber, 22-oil filling port, 23-cleaning port of multi-channel valve, 24-working output port of multi-channel valve, 25-oil filling chamber of multi-channel valve, 26-communication port of multi-channel valve. DETAILED DESCRIPTION

[0021] The following is a detailed description of the embodiments of the present invention. It should be emphasized that the following description is only exemplary and is not intended to limit the scope and application of the present invention. In the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other.

[0022] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element. In addition, connection can be used for fixing as well as for coupling or communication.

[0023] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0024] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the embodiments of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0025] like Figure 1-3As shown, a deep-sea multi-sequence in-situ seawater filtration and treatment device according to an embodiment of the present invention comprises a pump 5, a filter 1 for filtering impurities, a fixed liquid sample bag 2, a two-position three-way valve, a flow meter, an integrated chamber 4, a pressure sensor 7, an underwater control and energy supply unit, a multi-channel valve 8 and a filter membrane chamber 11, wherein: the two-position three-way valve and the flow meter are integrated and installed in the integrated chamber 4; the output port 18 of the two-position three-way valve is connected to the input port 19 of the flow meter, and the inlet of the pump 5 is connected to the output port 15 of the flow meter; an input channel 14 is provided between the pump 5 and the multi-channel valve 8, one end of the input channel 14 is communicated with the input port 13 of the multi-channel valve 8, and the other end of the input channel 14 is communicated with the output port of the pump 5, and the input channel 1 4 is connected to a pressure sensor 7; the multi-channel valve 8 has a plurality of output ports, a plurality of working channels are formed between the input port 13 of the multi-channel valve 8 and one end of the plurality of output ports of the multi-channel valve 8, the multi-channel valve 8 is controlled to rotate to switch each working channel, the other end of each output port of the multi-channel valve 8 is communicated with the inlet of the corresponding filter membrane cavity 11 through the filter channel 10, and each filter membrane cavity 11 is connected to the seawater outlet; the normally open inlet 17 of the two-position three-way valve is connected to the seawater inlet through the filter 1, and the normally closed inlet 16 of the two-position three-way valve is connected to the fixed liquid sample bag 2; the pump, the two-position three-way valve, the flow meter, the pressure sensor, and the multi-channel valve are respectively connected to the underwater control and energy supply unit. The underwater control and energy supply unit is used to collect data from the flow meter and the pressure sensor, and the underwater control and energy supply unit is used to control and power the pump, the multi-channel valve, and the two-position three-way valve respectively, so as to complete the forward and reverse rotation control of the multi-channel valve to realize the rotation of the multi-channel valve to switch the multiple working channels formed between the input port 13 of the multi-channel valve 8 and one end of the multiple output ports of the multi-channel valve 8, complete the channel switching of the two-position three-way valve (the two-position three-way valve is powered on to switch the normally open inlet 17 and the normally closed inlet 16), pump the water sample of the pump 5 and monitor the working conditions of each pipeline.

[0026] In this embodiment, the deep-sea multi-sequence in-situ seawater filtration and treatment device further includes a three-way valve 6 , and the pressure sensor 7 is connected to the passage of the input channel 14 through the three-way valve 6 .

[0027] In this embodiment, the two-position three-way valve, the flow meter, the pump, the pressure sensor, and the multi-channel valve all adopt an oil compensation mode and are independently packaged and can operate under deep-sea pressure. Each pipeline has no direct contact with the oil filled in the integrated cavity and there is no risk of contamination.

[0028] In this embodiment, the filter membrane cavity 11 includes a plurality of separate filter membrane devices to facilitate the removal, collection, and replacement of a single filter membrane. More preferably, these filter membrane devices can be stacked to simplify the spatial size of the equipment.

[0029] In this embodiment, the deep-sea multi-sequence in-situ seawater filtration and treatment device also includes a first one-way valve 3 and a second one-way valve 12, the normally closed inlet 16 of the two-position three-way valve is connected to the fixed liquid sample bag 2 through the first one-way valve 3, and each filter membrane cavity 11 is connected to the seawater outlet through the second one-way valve 12.

[0030] In this embodiment, further reference is made to Figure 2 The integrated chamber 4 is filled with oil, the two-position three-way valve and the flow meter are respectively installed at the lower and upper parts of the integrated chamber 4, the output port 18 of the two-position three-way valve, the input port 19 of the flow meter, the output port 15 of the flow meter and the normally closed inlet 16 of the two-position three-way valve are all located on the side of the integrated chamber, and the output port 18 of the two-position three-way valve and the input port 19 of the flow meter are directly connected through a short pipe.

[0031] In this embodiment, further reference is made to Figure 2 The cavity shell 21 of the integrated cavity is made of POM plastic, and the cavity of the integrated cavity is sealed by an oil sac or oil film 20 and external water or other liquid. The oil filled in the oil sac or oil film 20 and the integrated cavity 4 provides pressure compensation for the cavity shell 21 of the integrated cavity to cope with the pressure fluctuation of the deep sea; the top of the integrated cavity is provided with an oil filling port 22. The design of the integrated cavity 4 in the present invention can simplify the electrical output and save space size to meet the requirements of deep-sea equipment.

[0032] In this embodiment, further reference is made to Figure 3The multi-channel valve 8 comprises a multi-channel valve head 9 and a multi-channel valve oil filling chamber 25 which are connected to each other. The multi-channel valve 8 has one multi-channel valve input port 13, a multi-channel valve communication port 26 and a plurality of output ports. As the multi-channel valve 8 rotates, the output ports of the multi-channel valve 8 are respectively connected with the input port 13 of the multi-channel valve 8 to switch the working channels. The plurality of output ports of the multi-channel valve 8 are evenly distributed in the circumferential direction of the multi-channel valve head 9. One of the plurality of output ports is a cleaning port 23 of the multi-channel valve, and the rest are working output ports 24 of the multi-channel valve. Among the plurality of working channels formed between the input port 13 of the multi-channel valve 8 and one end of the plurality of output ports of the multi-channel valve 8, one is a cleaning channel, and the rest are sampling channels. An underwater driving motor (not shown) is provided in the multi-channel valve oil filling chamber 25 to drive the multi-channel valve 8 to rotate, so as to switch the cleaning channel and the sampling channels on the multi-channel valve. In this example, the underwater drive motor is placed in the oil-filling chamber 25 of the multi-channel valve, and pressure compensation is achieved through hydraulic oil to adapt to the deep-sea pressure environment. The communication port 26 of the multi-channel valve is connected to the energy supply unit through a watertight cable and underwater control. The communication port 26 of the multi-channel valve supplies power to the underwater drive motor and the optical sensor (not shown) used for multi-channel valve positioning in the oil-filling chamber 25 of the multi-channel valve. The underwater drive motor drives the multi-channel valve to rotate and convert the cleaning channel and each sampling channel on the multi-channel valve.

[0033] The working process of the deep-sea multi-sequence in-situ seawater filtration and treatment device in the above embodiment is as follows:

[0034] (1) When the deep-sea multi-sequence in-situ seawater filtration and treatment device dives to the working depth along with the deep-sea platform (such as a lander, ROV, etc.), the deep-sea platform sends a command to the underwater control and energy supply unit. At this time, the pump 5 starts to work and pumps seawater into the pipeline. The seawater enters the cleaning channel of the multi-channel valve 8 through the two-position three-way valve, flow meter, and pressure sensor 7 to clean the entire pipeline.

[0035] (2) After cleaning, the underwater control and energy supply unit controls the multi-channel valve 8 to rotate to the target working port. The pump 5 pumps a preset volume of seawater sample into the pipeline, wherein the filter 1 can isolate large particles and macroorganisms outside the pipeline. The seawater sample enters the filter membrane cavity 11 through the sampling channel of the multi-channel valve 8 at a preset flow rate. The filter membrane cavity 11 filters and enriches the deep-sea microorganisms and particles in the seawater sample flowing through on the filter membrane. The second one-way valve 12 provided between the outlet of the filter membrane cavity 11 and the seawater can prevent the backflow of seawater from contaminating the sample on the filter membrane.

[0036] (3) The flow meter detects the flow rate of filtered seawater, and calculates the flow rate of filtered seawater and the total amount of filtered seawater in real time. The pressure sensor 7 detects the fluid pressure of the liquid pipeline to prevent the multi-channel valve 8 from leaking due to excessive pipeline pressure, and at the same time feeds back to the pump 5 to drive the pipeline pressure to be controlled within a reasonable range. When the seawater flow rate is less than the set minimum flow rate, the total amount of seawater reaches the collection requirement, or the pipeline pressure exceeds the preset maximum value, the seawater sample is enriched, the pump 5 is turned off, and the normally closed inlet 16 of the two-position three-way valve is opened. The first one-way valve 3 provided between the fixed liquid sample bag 2 and the normally closed inlet 16 of the two-position three-way valve can prevent the seawater from reflux and contaminating the fixed liquid in the fixed liquid sample bag 2 due to pressure changes. When the pump 5 is turned on, the fixed liquid of the preset volume enters the corresponding filter membrane cavity 11 through the input port 13 of the multi-channel valve and the sampling channel, and the deep-sea sample enriched on the filter membrane is fixed and preserved, avoiding common deep-sea sampling problems such as continued reproduction of microorganisms and denaturation of particles.

[0037] (4) During the operation of the cleaning fluid, seawater sample, and fixed fluid, the flowmeter accurately controls the flow of the fluid. The underwater control and energy supply unit is connected to the flowmeter to obtain flow feedback, and automatically adjusts the rotation speed of the pump 5 to accurately control the flow; at the same time, the flowmeter obtains the total flow of each fluid. When each fluid reaches the preset volume, the underwater control and energy supply unit automatically controls the corresponding fluid operation. After the fixed fluid is pumped in, the pump 5 is turned off, and the multi-channel valve 8 is rotated to switch the internal flow path of the multi-channel valve 8 to the cleaning channel.

[0038] (5) According to the preset sampling time interval, steps (2)-(4) are repeated, so that microorganisms or particulate samples of different time series are enriched in each filter membrane cavity 11 through the working output ports 24 of different multi-channel valves. During the fluid working process, the underwater control and energy supply unit can monitor the pipeline flow, pipeline pressure, ambient temperature, remaining power and other data in real time to ensure the safe and reliable operation of the device.

[0039] Compared with the prior art, the deep-sea multi-sequence in-situ seawater filtration and treatment device provided by the present invention can avoid environmental changes, such as pressure and temperature, which damage the sample, and obtain in-situ deep-sea microorganisms and particulate matter samples in large volumes of water in multiple time series, and complete the in-situ preservation and fixation of the sample. Specifically, the above embodiment of the present invention can enrich a large number of microorganisms or particulate matter on a filter membrane according to the pore size of the filter membrane, so as to meet the high-throughput filtration; the above embodiment can use a fixing liquid to fix the DNA or RNA of microorganisms and particulate matter, meet the needs of in-situ fixation, avoid the influence of environmental factors such as temperature and pressure on the expression of gene information, and most realistically retain the gene expression information of microorganisms and the original properties of particulate matter in the deep-sea environment. Compared with the shortcomings of the current deep-sea microorganism sampling equipment, such as few sequences, low integration, large size, etc., and being easily affected by environmental variables, the independent packaging of each part of the present invention is easy to replace, and the overall integration is high. The use of multi-channel valves instead of traditional multiple two-position two-way valves not only saves the overall space while ensuring the number of channels, but also saves the energy supply demand, so that the present invention can work in the deep sea for a long time and is flexible and controllable. By using a multi-channel valve to switch each sampling channel, samples at multiple times and locations can be obtained in one dive, providing sufficient samples for subsequent research, which has great practicality. At the same time, the various parts of the present invention work flexibly and stably, with high integration, and can quickly and effectively obtain samples in multiple sequences and large throughput seawater.

[0040] The above content is a further detailed description of the present invention in combination with specific / preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, without departing from the concept of the present invention, it can also make several substitutions or modifications to these described embodiments, and these substitutions or modifications should be regarded as belonging to the protection scope of the present invention. In the description of this specification, the description of the reference terms "an embodiment", "some embodiments", "preferred embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily target the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In the absence of mutual contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples. Although the embodiments of the present invention and their advantages have been described in detail, it should be understood that various changes, substitutions and modifications can be made herein without departing from the scope of protection of the patent application.

Claims

1. A deep-sea multi-sequence in-situ seawater filtration and treatment device, characterized in that: It includes a pump, a filter, a fixed liquid sample bag, a two-position three-way valve, a flow meter, an integrated cavity, a pressure sensor, an underwater control and power supply unit, a multi-channel valve and a filter membrane cavity, wherein: The two-position three-way valve and the flow meter are integrated and installed in the integrated cavity; the output port of the two-position three-way valve is connected to the input port of the flow meter, and the inlet of the pump is connected to the output port of the flow meter; An input channel is provided between the pump and the multi-channel valve, one end of the input channel is communicated with the input port of the multi-channel valve, the other end of the input channel is communicated with the output port of the pump, and a pressure sensor is connected to the passage of the input channel; The multi-channel valve has a plurality of output ports, a plurality of working channels are formed between the input port of the multi-channel valve and one end of the plurality of output ports of the multi-channel valve, the multi-channel valve is controlled to rotate to switch each working channel, the other end of each output port is connected to the inlet of the corresponding filter membrane cavity through a filter channel, and each filter membrane cavity is connected to the seawater outlet; The normally open inlet of the two-position three-way valve is connected to the seawater inlet through a filter, and the normally closed inlet of the two-position three-way valve is connected to the fixative sample bag; The pump, the two-position three-way valve, the flow meter, the pressure sensor, and the multi-channel valve are respectively connected to the underwater control and power supply unit, and the underwater control and power supply unit is used to collect data from the flow meter and the pressure sensor, and to control and power the pump, the multi-channel valve, and the two-position three-way valve, respectively.

2. The deep-sea multi-sequence in-situ seawater filtration and treatment device according to claim 1, characterized in that: It also includes a three-way valve, and the pressure sensor is connected to the passage of the input channel through the three-way valve.

3. The deep-sea multi-sequence in-situ seawater filtration and treatment device according to claim 1, characterized in that: The two-position three-way valve, the flow meter, the pump, the pressure sensor, and the multi-channel valve all adopt an oil compensation mode and are independently packaged.

4. The deep-sea multi-sequence in-situ seawater filtration and treatment device according to claim 1, characterized in that: The filter membrane cavity includes a plurality of separate filter membrane devices.

5. The deep-sea multi-sequence in-situ seawater filtration and treatment device according to claim 1, characterized in that: It also includes a first one-way valve and a second one-way valve. The normally closed inlet of the two-position three-way valve is connected to the fixed liquid sample bag through the first one-way valve, and each filter membrane cavity is connected to the seawater outlet through the second one-way valve.

6. The deep sea multi-sequence in-situ seawater filtration and treatment device according to claim 1, characterized in that: The integrated cavity is filled with oil, the two-position three-way valve and the flowmeter are respectively installed at the lower part and the upper part of the integrated cavity, the output port of the two-position three-way valve, the input port of the flowmeter, the output port of the flowmeter and the normally closed inlet of the two-position three-way valve are all located on the side of the integrated cavity, and the output port of the two-position three-way valve and the input port of the flowmeter are directly connected by a pipeline.

7. The deep sea multi-sequence in-situ seawater filtration and treatment device according to claim 6, characterized in that: The material of the cavity shell of the integrated cavity is POM plastic, and the cavity of the integrated cavity is bounded and sealed by an oil sac or an oil film and the external liquid. The oil sac or the oil film and the oil filled in the integrated cavity provide pressure compensation for the cavity shell of the integrated cavity; an oil filling port is provided at the top of the integrated cavity.

8. The deep sea multi-sequence in-situ seawater filtration and treatment device according to claim 1, characterized in that: The multi-channel valve comprises a multi-channel valve head and a multi-channel valve oil filling chamber which are interconnected. The multi-channel valve has one multi-channel valve input port, one multi-channel valve communication port and a plurality of output ports; the plurality of output ports of the multi-channel valve are evenly distributed in the circumferential direction of the multi-channel valve head, one of the plurality of output ports is a multi-channel valve cleaning port, and the rest are working output ports of the multi-channel valve. Among the plurality of working channels formed between the input port of the multi-channel valve and one end of the plurality of output ports of the multi-channel valve, one is a cleaning channel, and the rest are sampling channels. An underwater driving motor is provided in the multi-channel valve oil filling chamber to drive the multi-channel valve to rotate, so as to convert the cleaning channel and each sampling channel on the multi-channel valve.

Citation Information

Patent Citations

  • In-situ automatic gathering and fixing device and method applied to microorganisms at all sea depths

    CN105420097A

  • In-situ multi-channel water body enriching, filtering and fixing device and method

    CN114367144A

  • Marine microorganism large-flux in-situ filtering device based on multi-channel circulating distributor

    CN114540185A

  • Full-deep-sea multi-sequence microorganism in-situ sampling device and method

    CN116396839A

  • Multi-sequence deep-sea microorganism in-situ enrichment and fixed sampling device and method

    CN118222383A

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