Sediment microorganism sample collector

Abstract

The invention discloses a sediment microorganism sample collector which comprises a collection rod assembly and a horizontal device, the collecting rod assembly is composed of an outer rod and a detachable inner container. The outer rod comprises a connecting and fixing part, an openable and closable part and a rotary excavating part; the detachable inner container is assembled in an inner cavity formed in the opening and closing part, and the horizontal device is assembled on the top face of the connecting and fixing part. The detachable inner container is convenient to replace, sterile operation in the sampling process is guaranteed, and it is guaranteed that a complete columnar sample is collected; the horizontal device can accurately judge whether sediment is vertically sampled during sampling, so that the collection accuracy is improved; the detachable inner container is convenient to mount and replace through the openable part, so that the sampling efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of soil sampling, in particular to a mangrove sediment microbial sample collector. Background Art

[0002] Since mangroves grow in intertidal zones and are periodically flooded and washed by seawater, the soil environment has special properties such as high salt, high humidity, low oxygen, and strong reducing properties, which brings many challenges to the sampling of mangrove soil. Traditional soil sampling methods, such as the soil drill method and the ring knife method, have certain limitations in mangrove soil sampling. For example, the soil drill method is easily disturbed by seawater during the sampling process, resulting in inaccurate sampling depth, and the soil drill is prone to rust and damage in a high-salt, high-humidity soil environment; the ring knife method requires cutting and compressing the soil during the sampling process, which can easily destroy the soil structure. It is difficult to obtain complete soil samples for the soft and high-humidity mangrove soil.

[0003] In the "A Soil Microorganism Determination Sampler" disclosed in the publication number CN202110530388.6, the device body includes a cylinder shell, a lifting mechanism and a cone, a semicircular pressing plate is provided at the upper end of the cylinder shell, and a cross plate is fixedly installed at the upper end of the semicircular pressing plate, a cross bar is fixedly installed at the cylinder mouth position of the lower end of the cylinder shell, and the cross bar crosses the inside of the cone, a pressure plate is fixedly installed at the middle position of the cross bar, and a leak hole is provided inside the pressure plate, and three groups of limit assemblies are equidistantly arranged near the middle and lower ends of the inner surface of the cylinder shell, and the three groups of limit assemblies are arranged horizontally; the limit assembly includes an L-shaped splint, a rotating rod and a semicircular spring piece, and the turning point position of the L-shaped splint is movably installed inside the cylinder shell through the rotating rod, and the number of semicircular spring pieces is two groups. The above invention can reduce the loss of collected soil, reduce the impact on the detection effect in the soil, reduce the entanglement of aquatic grasses, facilitate the sampling and collection of the device body, and improve the collection efficiency. However, for sampling environments such as mangroves, the above-mentioned device has a complex structure and is inconvenient to operate. In addition, for relatively soft soil, it is impossible to accurately determine whether the sediment is sampled vertically, and cannot provide more accurate inspection samples. Moreover, when using a collector for sampling, it is impossible to avoid contamination of samples by other microorganisms and cross-contamination between samples. Summary of the invention

[0004] In order to overcome the above defects in the prior art, a sediment microorganism sample collector is provided which has a simple structure and can quickly and efficiently obtain non-polluted sediment samples.

[0005] The technical solution of the present invention is as follows: a sediment microorganism sample collector, including a collection rod assembly and a horizontal device; the collection rod assembly consists of an outer rod and a detachable inner liner; the outer rod includes a connecting and fixing part, an openable and closable part and a rotary digging part; the detachable inner liner is assembled in an inner cavity provided in the openable and closable part, and the horizontal device is assembled on the top surface of the connecting and fixing part; the detachable inner liner is easy to replace, ensuring aseptic operation during sampling and ensuring the collection of complete columnar samples; the horizontal device can accurately determine whether vertical sampling is performed during sediment sampling, thereby improving the collection accuracy; the openable and closable part is convenient for installing and replacing the detachable inner liner, thereby improving the sampling efficiency.

[0006] Preferably, the openable and closable part is composed of two semicircular shells, one side of the two semicircular shells is connected by a hinge, and the other side is equipped with a fixing buckle. The two semicircular shells are connected by a hinge, making the opening and closing operation simple and quick. The detachable liner can be easily installed and disassembled, and the assembly efficiency is improved; and the design of the semicircular shell makes the cleaning work simple. After the collection is completed, the shell can be easily opened to clean and disinfect the detachable liner and shell.

[0007] Preferably, the rotary excavation part and the connection fixing part are respectively fixedly connected to the upper and lower ends of one of the semicircular shells. The fixed connection of the rotary excavation part and the connection fixing part with the semicircular shell makes the entire collector an integral whole, and the operator only needs to control the movement of the collection rod assembly through the horizontal device, without having to operate the rotary excavation part or the connection fixing part separately, which simplifies the operation process and improves the stability of the entire device.

[0008] Preferably, a positioning structure is provided on the top of the removable liner, and a fixing hole for engaging the positioning structure is provided on the top of the closable portion. The positioning structure ensures that the removable liner can be accurately placed in the closable portion, thereby avoiding inaccurate collection or shaking of the removable liner due to positional offset.

[0009] Preferably, a positioning plate for clamping the bottom of the removable liner is provided at the lower end of the openable and closable portion. The positioning plate and the positioning structure together form a device for double fixing up and down, thereby ensuring the stability of the removable liner during the collection process and reducing the risk of falling off or damage caused by shaking or vibration.

[0010] As a preferred embodiment, the inside and outside of the openable and closable part and the detachable inner container are marked with scale lines, which can be used for quantitative analysis of collected sediments and facilitate the recording and comparison of samples.

[0011] Preferably, the openable and closable portion and the detachable inner liner are both provided with air holes for assembling an air plug, and the air holes are provided above the scale line; the air plug makes the air pressure inside and outside the collector consistent, making it convenient for the collector to collect samples.

[0012] Preferably, the removable inner liner has the same shape as the openable and closable part, consisting of two semi-cylindrical outer shells, which facilitates the removal of samples, improves sampling efficiency, and facilitates cleaning of the inside of the removable inner liner to avoid contamination of the sample; the outer diameter of the removable inner liner is smaller than the inner diameter of the openable and closable part, which reduces wear and resistance caused by friction during assembly and disassembly.

[0013] Preferably, one side of the detachable inner liner is equipped with multiple push-type buckles, and the other side is connected by a hinge; the push-type buckles facilitate the quick disassembly of the detachable inner liner, and the multiple push-type buckles improve the overall sealing performance of the collector.

[0014] Preferably, the outer wall of the connecting and fixing part is provided with a rotating handle to facilitate the sampling operation of the operator.

[0015] Compared with the prior art, the present invention has the following beneficial effects: A sediment microbial sample collector can quickly penetrate sediments and effectively collect microbial samples therein through a rotary excavation part; the openable and closable part of the collection rod assembly allows a detachable inner liner to easily enter and exit the outer rod, can be operated by one person, is suitable for sampling areas in intertidal zones that are difficult to reach, is easy to operate in different environments, makes the sample collection process smoother, reduces the collection time, and improves the collection efficiency.

[0016] Furthermore, the detachable inner liner can be used for aseptic sampling to avoid cross-contamination between samples; the detachable inner liner can be reused to save costs.

[0017] Furthermore, the inner and outer sides of the openable and closable part and the detachable inner liner are marked with scale lines, which facilitate the operation of setting the depth and volume of the sample during sampling, thereby improving the accuracy of sampling.

[0018] Furthermore, the columnar samples collected by the removable liner can be stored intact in the laboratory, and the samples can be sterilely separated and stored in the laboratory to ensure that the samples are contaminated.

[0019] Other features and advantages of the present invention will be disclosed in detail in the following specific embodiments and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be further described below in conjunction with the accompanying drawings: Figure 1 It is a schematic diagram of the installation of a sediment microorganism sample collector of the present invention; Figure 2 It is a structural schematic diagram of the outer rod of the present invention; Figure 3 The structure side view of the outer rod of the present invention Figure 1 ; Figure 4 The structure side view of the outer rod of the present invention Figure 2 ; Figure 5 It is a structural cross-sectional view of the openable and closable part of the present invention; Figure 6 The structure of the detachable inner liner of the present invention is shown in FIG. Figure 1 ; Figure 7 The structure of the detachable inner liner of the present invention is shown in FIG. Figure 2 ; Figure 8 It is a structural cross-sectional view of the detachable inner liner of the present invention; The following are the descriptions of the reference numerals: Collection rod assembly 1, horizontal device 2, outer rod 11, detachable inner liner 12, connecting and fixing part 111, openable and closable part 112, rotary digging part 113, hinge 3, fixing buckle 4, press-type buckle 5, first positioning plate 112-2, positioning structure 121, second positioning plate 112-3, air plug 6, air hole 61, rotating handle 7. DETAILED DESCRIPTION

[0021] The technical solutions of the embodiments of the present invention are explained and described below in conjunction with the drawings of the embodiments of the present invention, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work are all within the protection scope of the present invention.

[0022] In the following description, terms such as "inside", "outside", "up", "down", "left", "right", etc. that indicate directions or positional relationships are only used to facilitate the description of the embodiments and simplify the description, and do not indicate or imply that the referred device or element must have a specific direction, be constructed and operate in a specific direction. Therefore, it should not be understood as a limitation of the present invention.

[0023] like Figures 1 to 8 As shown, a sediment microorganism sample collector includes a collection rod assembly 1 and a horizontal device 2; the collection rod assembly 1 is composed of an outer rod 11 and a detachable inner liner 12; the outer rod 11 includes a connecting and fixing portion 111, an openable and closable portion 112, and a rotary digging portion 113; the connecting and fixing portion 111 is used to be firmly connected to the horizontal device 2, and the horizontal device 2 is assembled on the top surface of the connecting and fixing portion 111 to ensure that the entire collector can be stably and accurately positioned to the target sediment area during operation; in this embodiment, the horizontal device 2 is a droplet level, and the droplet level indicates the horizontal state through bubbles or droplets in the liquid filled inside; when the level is placed on a completely horizontal surface, the bubbles or droplets in the liquid will be located in the center to indicate the horizontal state. If the surface is tilted, the position of the bubbles or droplets will change accordingly, thereby indicating the direction and angle of the tilt.

[0024] Among them, a rotary drilling structure is provided on the rotary drilling part 113. Through the design of the rotary drilling part 113, the collector can easily penetrate and stir the sediment, thereby efficiently collecting microbial samples; in this embodiment, the bottom end of the rotary drilling part 113 is a serrated structure, and the side of the rotary drilling part 113 is a hollow spiral structure, so that the rotary drilling part 113 can quickly penetrate various types of ground.

[0025] Among them, the openable and closable part 112 is located below the connecting and fixing part and above the rotary digging part 113, and is the carrier of the removable inner liner 12; the removable inner liner 12 is assembled in the inner cavity provided in the openable and closable part 112, and a positioning structure 121 is provided on the top of the removable inner liner 12, and a first positioning plate 112-2 for clamping the positioning structure 121 is provided on the top of the openable and closable part 112, and a second positioning plate 112-3 is provided at the lower end of the openable and closable part 112, and the upper and lower ends of the removable inner liner 12 are fixed in the openable and closable part 112 by the first positioning plate 112-2 and the second positioning plate 112-3, thereby improving the stability of the removable inner liner 12.

[0026] Furthermore, the closable portion 112 is composed of two semi-cylindrical shells, one side of the two semi-cylindrical shells is connected by a hinge 3, and the other side is equipped with a fixing buckle 4. The rotary digging portion 113 and the connecting and fixing portion 111 are respectively fixedly connected to the upper and lower ends of one of the semi-cylindrical shells. It is convenient to install and remove the removable inner liner 12, improve the sampling efficiency, and facilitate operators to quickly carry out operations in the sampling area; it is also convenient to replace the removable inner liner 12, and it is convenient to take out the removable inner liner 12 after sampling, and to clean and maintain the removable inner liner 12.

[0027] The removable inner liner 12 has the same shape as the openable and closable part 112, and is composed of two semi-cylindrical outer shells; it is convenient to take out the sample; the outer diameter of the removable inner liner 12 is smaller than the inner diameter of the openable and closable part 112. During the assembly and disassembly process, this design makes the operation smoother and reduces the wear and resistance caused by friction. One side of the removable inner liner 12 is equipped with multiple push-type buckles 5, and the other side is connected by a hinge 3; the push-type buckle allows the operator to quickly and easily open or close the removable inner liner 12, which is convenient for taking out the sample and cleaning the inside of the removable inner liner 12 to avoid sample contamination during the next sampling.

[0028] Furthermore, the detachable inner liner 12 is made of high molecular weight polypropylene material, which can be washed, disinfected and sterilized after sampling once, and can be reused, thereby reducing the cost of disposable utensils for long-term sampling.

[0029] Among them, the inside and outside of the openable and closable part 112 and the removable inner liner 12 are marked with scale lines. The scale lines enable the operator to intuitively read the depth information during the collection process. Whether inserting sediment or extracting samples, the depth of collection can be accurately controlled by observing the scale lines to ensure that samples of the target depth are collected. The openable and closable part 112 and the removable inner liner 12 are both provided with air holes 61 for assembling the air plug 6. The air holes 61 are opened above the scale lines. The air plug 6 and the air holes 61 make the air pressure inside and outside the collector consistent, so that the collector will not be unable to collect due to excessive internal pressure during sampling, which facilitates the collector to collect samples.

[0030] Furthermore, a rotating handle 7 is installed on the outer wall of the connecting and fixing portion 111 to facilitate the operator to perform rotary drilling operations.

[0031] Specific sampling implementation method: When the operator arrives at the sampling point, first, it is necessary to open the closable part 112 on the outer rod 11, then put the sterilized removable inner liner 12 into it, and then close the closable part 112 and fix it with the fixing buckle 4. At the sampling point, it is necessary to make careful adjustments to it with the help of the horizontal device 2 to ensure that it is in a vertical state. After adjustment, insert and open the air plug 6 vertically, and then start the rotary digging operation. It is worth noting that the scale lines on the outer rod 11 can clearly show the sampling depth. In actual operation, try to make the sampling depth a few centimeters more than the pre-conceived depth. When the sampling is completed, plug the air plug 6 and lift it up, then open the outer rod 11, and take out the removable inner liner 12 to end the sampling; when arriving at the next sampling point, replace another removable inner liner 12, and follow the above-mentioned operation process.

[0032] When selecting the sampling depth during operation, if the expected depth is 20 cm, then in order to ensure the integrity and effectiveness of the sample, the actual sampling depth is best to reach 22 cm or 23 cm.

[0033] After sampling, return to the laboratory, open the removable liner 12, use a sterilized knife or tweezers to transfer sediment samples of different depths to a sterile sample storage tube, and wait for subsequent experimental processing. The removable liner after use can be cleaned and sterilized at high temperature, waiting for the next sampling. During the sampling process, each sampling point only needs to replace the removable liner 12 once, and the removable liner 12 can be reused, which is easy to operate, ensures the integrity and pollution-free of the sample, and reduces the cost of disposable utensils for long-term sampling.

[0034] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that the present invention includes but is not limited to the contents described in the drawings and the above specific embodiments. Any modification that does not deviate from the functional and structural principles of the present invention will be included in the scope of the claims.

Claims

1. A sediment microbial sample collector, characterized in that: The invention comprises a collecting rod assembly (1) and a horizontal device (2); the collecting rod assembly (1) comprises an outer rod (11) and a detachable inner liner (12); the outer rod (11) comprises a connecting and fixing portion (111), an openable and closable portion (112) and a rotary digging portion (113); the detachable inner liner (12) is mounted in an inner cavity provided in the openable and closable portion (112); and the horizontal device (2) is mounted on the top surface of the connecting and fixing portion (111).

2. A sediment microorganism sample collector according to claim 1, characterized in that: The closable portion (112) is composed of two semi-cylindrical shells, one side of the two semi-cylindrical shells is connected by a hinge (3), and the other side is provided with a fixing buckle (4).

3. A sediment microorganism sample collector according to claim 1, characterized in that: The rotary drilling portion (113) and the connecting and fixing portion (111) are respectively fixedly connected to the upper and lower ends of one of the semi-cylindrical shells.

4. A sediment microorganism sample collector according to claim 1, characterized in that: A positioning structure (121) is provided on the top of the detachable inner liner (12), and a first positioning plate (112-2) for clamping the positioning structure (121) is provided on the top of the openable and closable portion (112).

5. The sediment microorganism sample collector according to claim 1, characterized in that: The lower end of the openable and closable portion (112) is provided with a second positioning plate (112-3) for clamping the bottom end of the detachable inner liner (12).

6. The sediment microorganism sample collector according to claim 1, characterized in that: The inner and outer sides of the openable and closable portion (112) and the detachable inner liner (12) are both marked with scale lines.

7. A sediment microorganism sample collector according to claim 1, characterized in that: The openable and closable portion (112) and the detachable inner liner (12) are both provided with an air hole (61) for assembling the air plug (6), and the air hole (61) is provided above the scale line.

8. The sediment microorganism sample collector according to claim 1, characterized in that: The detachable inner liner (12) has the same shape as the closable portion (112), and is composed of two semi-cylindrical outer shells; the outer diameter of the detachable inner liner (12) is smaller than the inner diameter of the closable portion (112).

9. The sediment microorganism sample collector according to claim 1, characterized in that: One side of the detachable inner liner (12) is equipped with a plurality of press-type buckles (5), and the other side is connected via a hinge (3).

10. The sediment microorganism sample collector according to claim 1, characterized in that: The outer wall of the connecting and fixing portion (111) is provided with a rotating handle (7).

Images