Automatic bacterial liquid coating equipment

Abstract

The invention relates to the technical field of experimental equipment, in particular to automatic bacterial liquid coating equipment which comprises a shell, the interior of the shell is divided into an upper cavity and a lower cavity through a partition plate, a feeding mechanism and a discharging mechanism which are used for containing culture plates are arranged in the lower cavity, and a conveying mechanism used for conveying the culture plates is arranged in the upper cavity. A cover opening and closing mechanism used for taking and placing an upper cover of the culture plate is arranged on one side of the conveying mechanism, a ball adding mechanism used for putting balls into the culture plate is arranged above the conveying mechanism, and a liquid transferring mechanism used for putting reagents into the culture plate is arranged on one side of the ball adding mechanism; one end of the feeding mechanism and one end of the discharging mechanism penetrate through the partition plate and stretch into the upper cavity. By means of the feeding mechanism, the conveying mechanism, the cover opening and closing mechanism, the ball adding mechanism, the liquid transferring mechanism and the discharging mechanism, full-automatic operation is achieved, manual intervention is effectively reduced, bacterial liquid coating can be efficiently completed, and the experiment efficiency is effectively improved.

Description

Technical Field

[0001] The invention relates to the technical field of experimental equipment, in particular to an automatic bacterial liquid coating device. Background Art

[0002] At present, there is no suitable automated flat-plate coating equipment on the market; coating now mainly relies on the traditional manual mode, which consumes a lot of manpower and time costs; in addition, in addition to the above-mentioned problems, the manual mode has a potential risk of sample confusion to a certain extent, which will hinder the advancement of downstream applications, thereby delaying the progress of the experiment and causing unnecessary waste; in view of the shortcomings of the prior art and market demand, the present invention designs a suitable automated flat-plate coating equipment. Summary of the invention

[0003] The purpose of the present invention is to provide an automated bacterial liquid coating device to solve the problems existing in the above-mentioned prior art.

[0004] To achieve the above-mentioned purpose, the present invention provides the following scheme: an automated bacterial liquid coating device, comprising a shell, wherein the shell is divided into an upper chamber and a lower chamber by a partition, the lower chamber is provided with a loading mechanism and a unloading mechanism for placing a culture plate, the upper chamber is provided with a conveying mechanism for conveying the culture plate, one side of the conveying mechanism is provided with a switch cover mechanism for taking and placing the upper cover of the culture plate, the upper part of the conveying mechanism is provided with a round bead adding mechanism for putting round beads into the culture plate, and one side of the round bead adding mechanism is provided with a pipetting mechanism for putting reagents into the culture plate; one end of the loading mechanism and the unloading mechanism penetrates the partition and extends into the upper chamber.

[0005] Preferably, the loading mechanism includes a silo turntable rotatably connected to the lower cavity, a plurality of first silo units for placing culture plates are fixedly connected to the silo turntable, a first electric clamp for grabbing the culture plate is arranged above the first silo unit, the first electric clamp is fixedly connected to a first Z-axis lifting module for driving the first electric clamp to lift and lower, the first Z-axis lifting module is fixedly connected to a first X-axis transport module for driving the first Z-axis lifting module to move horizontally, the first electric clamp, the first Z-axis lifting module and the first X-axis transport module are arranged in the upper cavity, and the first X-axis transport module is fixedly connected to the top surface of the partition.

[0006] Preferably, the conveying mechanism includes a fixed plate fixedly connected to the middle part of the top surface of the partition, the fixed plate is provided with a plurality of culture plate slots for placing culture plates and a plurality of culture plate cover slots for placing culture plate upper covers, a horizontal conveying module is arranged below the fixed plate, and the horizontal conveying module is fixedly connected to one end of the fixed plate with a plurality of lifting plates for moving culture plates and culture plate upper covers.

[0007] Preferably, the cover opening and closing mechanism comprises two sets of picking parts, the picking parts for taking the cover are arranged close to the loading mechanism arranged in the upper cavity, and the picking parts for buckling the cover are arranged close to the unloading mechanism arranged in the upper cavity.

[0008] Preferably, the picking part includes a bracket fixedly connected to the partition, the top of the bracket is fixedly connected to a first Y-axis displacement module, the moving end of the first Y-axis displacement module is fixedly connected to a Z-axis displacement module, and the moving end of the Z-axis displacement module is fixedly connected to an electric suction cup.

[0009] Preferably, the ball adding mechanism includes a hopper arranged above the fixed plate, the hopper is connected to the feed end of the ball brushing motor, the discharge end of the ball brushing motor is connected to a ball pushing plate, the ball pushing plate is connected to a bead filter plate, and the bead filter plate is arranged above the culture plate slot.

[0010] Preferably, the pipetting mechanism includes a support frame fixedly connected to the top surface of the partition, a second Y-axis displacement module is fixedly connected to the top of the support frame, a mobile end of the second Y-axis displacement module is fixedly connected to a pipette, an X-axis displacement module is arranged below the pipette, the mobile end of the X-axis displacement module is fixedly connected to a tip box and a deep-well plate, the tip box and the deep-well plate are arranged below the pipette, and a tip waste box is fixedly connected to one side of the X-axis displacement module.

[0011] Preferably, the unloading mechanism includes a shaker fixedly connected to the bottom of the lower chamber, the top surface of the shaker is fixedly connected to a second closed-loop stepper motor, the output shaft of the second closed-loop stepper motor is transmission-connected to the center of a support plate, and a plurality of second silo units for placing culture plates are fixedly connected to the support plate.

[0012] Preferably, a second electric gripper for grasping the culture plate is arranged above the second silo unit, the second electric gripper is fixedly connected to a second Z-axis lifting module for driving the second electric gripper to lift and lower, the second Z-axis lifting module is fixedly connected to a second X-axis transport module for driving the second Z-axis lifting module to move horizontally, the second electric gripper, the second Z-axis lifting module and the second X-axis transport module are arranged in the upper cavity, and the second X-axis transport module is fixedly connected to the top surface of the partition.

[0013] Preferably, an observation window is embedded in the upper part of one side of the shell, a front cabinet door is arranged below the observation window, the front cabinet door is installed on the shell, and a plurality of side cabinet doors are installed on the other side of the shell.

[0014] The present invention discloses the following technical effects:

[0015] The present invention realizes full-automatic operation through a feeding mechanism, a conveying mechanism, a cover opening and closing mechanism, a ball adding mechanism, a liquid transfer mechanism and a feeding mechanism, effectively reduces manual intervention and effectively improves experimental efficiency; at the same time, the present invention avoids human operation errors and improves the accuracy of experimental results through precise liquid transfer and transportation of culture plates; and effectively reduces the risk of sample contamination caused by environmental or human errors through fully enclosed operation and fully automated processes in a shell; and greatly reduces labor and time costs through automated operation, and can efficiently complete the coating of bacterial liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 This is a schematic diagram of the appearance structure of the present invention;

[0018] Figure 2 It is a schematic diagram of the overall internal structure of the present invention;

[0019] Figure 3 It is a schematic diagram of the internal top view structure of the present invention;

[0020] Figure 4 It is a schematic diagram of the structure of the feeding mechanism of the present invention;

[0021] Figure 5 It is a schematic diagram of the structure of the conveying mechanism of the present invention;

[0022] Figure 6 This is a schematic diagram of the structure of the switch cover mechanism of the present invention;

[0023] Figure 7 This is a schematic diagram of the structure of the ball adding mechanism of the present invention;

[0024] Figure 8 It is a schematic diagram of the structure of the liquid transfer mechanism of the present invention;

[0025] Fig. 9 It is a schematic diagram of the structure of the blanking mechanism of the present invention;

[0026] Among them, 1. feeding mechanism; 2. conveying mechanism; 3. opening and closing cover mechanism; 4. ball adding mechanism; 5. transfer mechanism; 6. unloading mechanism; 7. shell; 8. partition; 9. culture plate; 11. silo turntable; 12. first silo monomer; 13. first closed-loop stepper motor; 14. Z-axis lifting module; 15. X-axis transport module; 16. electric gripper; 21. fixing plate; 22. culture plate slot; 23. lifting plate; 24. culture plate cover slot; 25. horizontal conveying module; 31. Bracket; 32, first Y-axis displacement module; 33, electric suction cup; 34, Z-axis displacement module; 41, hopper; 42, ball brush motor; 43, ball push plate; 44, bead filter plate; 51, support frame; 52, second Y-axis displacement module; 53, pipette; 54, tip box; 55, deep well plate; 56, tip waste box; 57, X-axis displacement module; 61, shaker; 62, support plate; 63, second silo unit; 71, observation window; 72, front cabinet door; 73, side cabinet door. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] Reference Figure 1-Figure 9 The present invention provides an automated bacterial liquid coating device, comprising a shell 7, the shell 7 is divided into an upper chamber and a lower chamber by a partition 8, a loading mechanism 1 and a unloading mechanism 6 for placing a culture plate 9 are arranged in the lower chamber, a conveying mechanism 2 for conveying the culture plate 9 is arranged in the upper chamber, a switch cover mechanism 3 for taking and placing the upper cover of the culture plate 9 is arranged on one side of the conveying mechanism 2, a bead adding mechanism 4 for putting beads into the culture plate 9 is arranged above the conveying mechanism 2, and a pipetting mechanism 5 for putting reagents into the culture plate 9 is arranged on one side of the bead adding mechanism 4; one end of the loading mechanism 1 and the unloading mechanism 6 penetrates the partition 8 and extends into the upper chamber.

[0030] The present invention realizes full-automatic operation through the feeding mechanism 1, the conveying mechanism 2, the cover opening and closing mechanism 3, the ball adding mechanism 4, the pipetting mechanism 5 and the unloading mechanism 6, effectively reduces human intervention and effectively improves the experimental efficiency; at the same time, the present invention avoids human operation errors and improves the accuracy of the experimental results through precise pipetting and transportation of the culture plate 9; and through fully enclosed operation and fully automated process in the shell 7, effectively reduces the risk of sample contamination caused by environmental or human errors; and through automated operation, the manpower and time costs are greatly reduced, and the coating of the bacterial solution can be completed efficiently.

[0031] In order to realize automatic operation, the loading mechanism 1, the conveying mechanism 2, the opening and closing cover mechanism 3, the ball adding mechanism 4, the pipetting mechanism 5 and the unloading mechanism 6 are electrically connected to the main control system, and the loading mechanism 1, the conveying mechanism 2, the opening and closing cover mechanism 3, the ball adding mechanism 4, the pipetting mechanism 5 and the unloading mechanism 6 are coordinated and controlled by the main control system to realize fully automatic operation.

[0032] A further optimized solution is that the loading mechanism includes a silo turntable 11 rotatably connected in the lower cavity, a plurality of first silo monomers 12 for placing culture plates 9 are fixedly connected to the silo turntable 11, a first electric clamp 16 for grabbing the culture plate 9 is arranged above the first silo monomer 12, the first electric clamp 16 is fixedly connected to a first Z-axis lifting module 14 for driving the first electric clamp 16 to rise and fall, the first Z-axis lifting module 14 is fixedly connected to a first X-axis transport module 15 for driving the first Z-axis lifting module 14 to move horizontally, the first electric clamp 16, the first Z-axis lifting module 14, and the first X-axis transport module 15 are arranged in the upper cavity, and the first X-axis transport module 15 is fixedly connected to the top surface of the partition 8.

[0033] In order to ensure that the culture plate 9 can be stably placed in the first silo unit 12, the first silo unit 12 includes a plurality of round rods, which are evenly arranged around the culture plate 9 so that the culture plate 9 can be raised and lowered along the round rods and a plurality of culture plates 9 can be stacked in each first silo unit 12.

[0034] The bottom center transmission of the silo turntable 11 is connected to a first closed-loop stepper motor 13, which drives the silo turntable 11 to rotate, and the silo turntable 11 drives the first silo monomer 12 to move, so that the first silo monomer 12 can be directly below the first electric clamp 16, so that the first electric clamp 16 can effectively clamp the culture plate 9 and transport the culture plate 9 to the conveying mechanism 2.

[0035] A further optimized solution is that the conveying mechanism 2 includes a fixed plate 21 fixedly connected to the middle part of the top surface of the partition 8, and the fixed plate 21 is provided with a plurality of culture plate slots 22 for placing the culture plates 9 and a plurality of culture plate cover slots 24 for placing the upper covers of the culture plates 9, and a horizontal conveying module 25 is arranged below the fixed plate 21, and the horizontal conveying module 25 is fixedly connected to one end of the fixed plate 21 with a plurality of lifting plates 23 for moving the culture plates 9 and the upper covers of the culture plates.

[0036] The first electric clamp 16 places the culture plate 9 into the culture plate slot 22, first lifts the culture plate 9 through the lifting plate 23, and then drives the lifting plate 23 to move through the horizontal conveying module 25, so that the culture plate 9 moves from one culture plate slot 22 to another adjacent culture plate slot 22, and then the next step of work can be carried out on the culture plate 9. The next step of the work process is: open the cover, add beads, add liquid, and close the cover.

[0037] Several culture plate slots 22 are interconnected, and the number of lifting plates 23 below the several culture plate slots 22 is two less than the number of culture plate slots 22, so that the lifting plates 23 can move back and forth in the several culture plate slots 22, so that the lifting plates 23 can drive the culture plates 9 to change positions in sequence.

[0038] Several culture plate cover slots 24 are interconnected, and the number of lifting plates 23 below the several culture plate cover slots 24 is two less than the number of culture plate cover slots 24, so that the lifting plates 23 can move back and forth in the several culture plate cover slots 24, so that the lifting plates 23 can drive the upper covers of the culture plates 9 to change positions in sequence.

[0039] According to a further optimization scheme, the cover opening and closing mechanism 3 includes two sets of picking parts. The picking parts for taking the cover are arranged close to the loading mechanism 1 arranged in the upper cavity, and the picking parts for buckling the cover are arranged close to the unloading mechanism 6 arranged in the upper cavity.

[0040] A further optimized solution is that the picking part includes a bracket 31 fixedly connected to the partition 8, the top of the bracket 31 is fixedly connected to the first Y-axis displacement module 32, the moving end of the first Y-axis displacement module 32 is fixedly connected to the Z-axis displacement module 34, and the moving end of the Z-axis displacement module 34 is fixedly connected to the electric suction cup 33.

[0041] In order to prevent the culture plate 9 from shaking during the process of removing the cover, a clamping cylinder is installed in each culture plate slot 22, and the culture plate 9 is clamped in the culture plate slot 22 by the clamping cylinder. At the same time, in order to detect whether there is a culture plate 9 in each culture plate slot 22, a sensor is also provided in each culture plate slot 22 for detecting whether there is a culture plate 9 in the culture plate slot 22.

[0042] The electric suction cup 33 is driven by the first Y-axis displacement module 32 and the Z-axis displacement module 34 to position and absorb the upper cover of the culture plate 9 , and the upper cover of the culture plate 9 is placed in the culture plate cover groove 24 .

[0043] A further optimized solution is that the ball adding mechanism 4 includes a hopper 41 arranged above the fixed plate 21, the hopper 41 is connected to the feed end of the ball brushing motor 42, the discharge end of the ball brushing motor 42 is connected to the ball pushing plate 43, the ball pushing plate 43 is connected to the bead filter plate 44, and the bead filter plate 44 is arranged above the culture plate slot 22.

[0044] The beads enter the ball brushing motor 42 through the hopper 41. The ball brushing motor 42 generates power through rotation, and brushes the cylinder onto the filter bead plate 44 through the brush. Then, the ball push plate 43 moves, causing the cylinder to fall into the culture plate 9.

[0045] A further optimized solution is that the pipetting mechanism 5 includes a support frame 51 fixedly connected to the top surface of the partition 8, a second Y-axis displacement module 52 is fixedly connected to the top of the support frame 51, a mobile end of the second Y-axis displacement module 52 is fixedly connected to a pipette 53, an X-axis displacement module 57 is arranged below the pipette 53, a mobile end of the X-axis displacement module 57 is fixedly connected to a tip box 54 and a deep well plate 55, the tip box 54 and the deep well plate 55 are arranged below the pipette 53, and a tip waste box 56 is fixedly connected to one side of the X-axis displacement module 57.

[0046] The tip box 54 and the deep well plate 55 are moved by the X-axis displacement module 57, and the pipette 53 is driven by the second Y-axis displacement module 52 to add liquid into the culture plate 9, and then the upper cover of the culture plate 9 is installed on the culture plate 9 by another picking part.

[0047] A further optimized solution is that the unloading mechanism 6 includes a shaker 61 fixedly connected to the bottom of the lower chamber, the top surface of the shaker 61 is fixedly connected to a second closed-loop stepper motor, the output shaft of the second closed-loop stepper motor is transmission-connected to the center of a support plate 62, and a plurality of second silo monomers 63 for placing culture plates 9 are fixedly connected to the support plate 62.

[0048] A further optimized solution is that a second electric gripper for grabbing the culture plate 9 is arranged above the second silo monomer 63, the second electric gripper is fixedly connected to a second Z-axis lifting module for driving the second electric gripper to lift and lower, the second Z-axis lifting module is fixedly connected to a second X-axis transport module for driving the second Z-axis lifting module to move horizontally, the second electric gripper, the second Z-axis lifting module and the second X-axis transport module are arranged in the upper cavity, and the second X-axis transport module is fixedly connected to the top surface of the partition 8.

[0049] Through the second Z-axis lifting module and the second X-axis transport module, the second electric clamp can transport the culture plate 9 in the culture plate slot 22 to the second silo monomer 63. When one second silo monomer 63 is full, the second closed-loop stepper motor is used to drive the support plate 62 to rotate to load the other second silo monomer 63 with culture plates 9. When all the culture plates 9 are placed in the second silo monomer 63, the shaker 61 works to shake the culture plates 9.

[0050] According to a further optimization scheme, an observation window 71 is embedded in the upper part of one side of the shell 7, a front cabinet door 72 is provided below the observation window 71, the front cabinet door 72 is installed on the shell 7, and a plurality of side cabinet doors 73 are installed on the other side of the shell 7.

[0051] In order to facilitate opening and closing of the observation window 71 , a first door is installed on the housing 7 , and the observation window 71 is embedded in the first door.

[0052] In order to facilitate opening the shell 7 to perform maintenance inside the shell 7 , a plurality of second doors are installed on the shell 7 , so that any part of the shell 7 can be opened through the second doors.

[0053] The first door and the second door are sliding doors, lift doors, etc.

[0054] By opening the front cabinet door 72 and the side cabinet door 73 , it is convenient to install the deep well plate 55 and the tip box 54 on the X-axis displacement module 57 , place the glass beads in the hopper 41 , and place the required number of culture plates 9 in the first silo unit 12 .

[0055] The first Z-axis lifting module 14, the first X-axis transport module 15, the first Y-axis displacement module 32, the Z-axis displacement module 34, the second Y-axis displacement module 52, the second Z-axis lifting module and the second X-axis transport module are driven by a brake stepper motor to operate with a precision linear ball screw.

[0056] Working process:

[0057] S1. Sample preparation: After UV disinfection of the inside of the housing 7 for 30 minutes, install the deep well plate 55 and the tip box 54 on the X-axis displacement module 57, place the glass beads in the hopper 41, and place the required number of culture plates 9 in the first silo monomer 12.

[0058] S2. Injection: The first Z-axis lifting module 14 and the first X-axis transport module 15 drive the first electric gripper 16 to grab the culture plate 9 in the first silo monomer 12 and place it in the culture plate slot 22. The culture plate 9 is first lifted by the lifting plate 23, and then the lifting plate 23 is driven to move by the horizontal conveying module 25 to move the culture plate 9 from one culture plate slot 22 to another adjacent culture plate slot 22. The next step of work on the culture plate 9 can be carried out. The next step of the work process is: open the cover, add beads, add liquid, and close the cover.

[0059] S3. Open the cover: The electric suction cup 33 is driven by the first Y-axis displacement module 32 and the Z-axis displacement module 34 to position and absorb the upper cover of the culture plate 9, and the upper cover of the culture plate 9 is placed in the culture plate cover groove 24; at this time, the horizontal conveying module 25 drives the lifting plate 23 to move, so that the culture plate 9 moves from one culture plate groove 22 to another adjacent culture plate groove 22, so that the culture plate 9 is under the filter bead plate 44.

[0060] S4. Add glass beads: the beads enter the ball brushing motor 42 through the hopper 41. The ball brushing motor 42 generates power through rotation, and brushes the cylinder onto the filter bead plate 44 through the brush. Then, the ball push plate 43 moves to make the cylinder fall into the culture plate 9, and 10-15 beads are added to each culture plate 9. At this time, the horizontal conveying module 25 drives the lifting plate 23 to move, so that the culture plate 9 moves from one culture plate slot 22 to another adjacent culture plate slot 22, so that the culture plate 9 is under the pipette 53.

[0061] S5. Add bacterial liquid: The tip box 54 and the deep well plate 55 are moved by the X-axis displacement module 57, and the pipette 53 is driven by the second Y-axis displacement module 52 to add liquid into the culture plate 9, and then the upper cover of the culture plate 9 is installed on the culture plate 9 by another picking part.

[0062] S6. Closing the cover: Another picking part works, and drives the electric suction cup 33 to position and suck the upper cover of the culture plate 9 in the culture plate cover slot 24 through the first Y-axis displacement module 32 and the Z-axis displacement module 34, and buckles the upper cover of the culture plate 9 on the culture plate 9; at this time, the horizontal conveying module 25 drives the lifting plate 23 to move, so that the culture plate 9 moves from one culture plate slot 22 to another adjacent culture plate slot 22, so that the culture plate 9 is in the last culture plate slot 22.

[0063] S7. Sample delivery: The second electric gripper is able to transport the culture plate 9 in the culture plate slot 22 to the second silo monomer 63 through the second Z-axis lifting module and the second X-axis transport module. When one second silo monomer 63 is full, the second closed-loop stepper motor is used to drive the support plate 62 to rotate and load the other second silo monomer 63 with culture plates 9 until all the culture plates 9 are placed in the second silo monomer 63.

[0064] S8. Bacterial solution coating: The shaker 61 works to shake the culture plate 9 in the front, back, left and right directions, so that the beads in the culture plate 9 are evenly coated with the bacterial solution by moving, and the samples are collected manually after completion.

[0065] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0066] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. An automated bacterial liquid coating device, characterized in that: The invention comprises a shell (7), wherein the shell (7) is divided into an upper chamber and a lower chamber by a partition (8), wherein a loading mechanism (1) and a unloading mechanism (6) for placing a culture plate (9) are arranged in the lower chamber, wherein a conveying mechanism (2) for conveying the culture plate (9) is arranged in the upper chamber, wherein a cover opening and closing mechanism (3) for taking and placing the upper cover of the culture plate (9) is arranged on one side of the conveying mechanism (2), wherein a ball adding mechanism (4) for putting round beads into the culture plate (9) is arranged above the conveying mechanism (2), wherein a liquid transfer mechanism (5) for putting reagent into the culture plate (9) is arranged on one side of the ball adding mechanism (4); One end of the loading mechanism (1) and the unloading mechanism (6) penetrates through the partition plate (8) and extends into the upper cavity.

2. The automated bacterial liquid coating equipment according to claim 1, characterized in that: The loading mechanism comprises a silo turntable (11) rotatably connected to the lower chamber, a plurality of first silo units (12) for placing culture plates (9) are fixedly connected to the silo turntable (11), a first electric clamp (16) for grabbing the culture plate (9) is arranged above the first silo unit (12), the first electric clamp (16) is fixedly connected to a first Z-axis lifting module (14) for driving the first electric clamp (16) to rise and fall, the first Z-axis lifting module (14) is fixedly connected to a first X-axis transport module (15) for driving the first Z-axis lifting module (14) to move horizontally, the first electric clamp (16), the first Z-axis lifting module (14) and the first X-axis transport module (15) are arranged in the upper chamber, and the first X-axis transport module (15) is fixedly connected to the top surface of the partition (8).

3. The automated bacterial liquid coating equipment according to claim 1, characterized in that: The conveying mechanism (2) comprises a fixed plate (21) fixedly connected to the middle part of the top surface of the partition (8), the fixed plate (21) is provided with a plurality of culture plate slots (22) for placing culture plates (9) and a plurality of culture plate cover slots (24) for placing culture plate (9) covers, a horizontal conveying module (25) is arranged below the fixed plate (21), and a plurality of lifting plates (23) for moving culture plates (9) and culture plate covers are fixedly connected to one end of the horizontal conveying module (25) facing the fixed plate (21).

4. The automated bacterial liquid coating equipment according to claim 1, characterized in that: The lid opening and closing mechanism (3) comprises two groups of picking parts, wherein the picking parts for taking the lid are arranged close to the loading mechanism (1) arranged in the upper cavity, and the picking parts for locking the lid are arranged close to the unloading mechanism (6) arranged in the upper cavity.

5. The automated bacterial liquid coating device according to claim 4, characterized in that: The picking-up portion comprises a bracket (31) fixedly connected to the partition (8); a first Y-direction displacement module (32) is fixedly connected to the top of the bracket (31); a Z-direction displacement module (34) is fixedly connected to the moving end of the first Y-direction displacement module (32); and an electric suction cup (33) is fixedly connected to the moving end of the Z-direction displacement module (34).

6. The automated bacterial liquid coating device according to claim 3, characterized in that: The ball adding mechanism (4) includes a hopper (41) arranged above the fixed plate (21), the hopper (41) is connected to the feed end of the ball brushing motor (42), the discharge end of the ball brushing motor (42) is connected to the ball pushing plate (43), the ball pushing plate (43) is connected to the bead filter plate (44), and the bead filter plate (44) is arranged above the culture plate slot (22).

7. The automated bacterial liquid coating device according to claim 1, characterized in that: The pipetting mechanism (5) comprises a support frame (51) fixedly connected to the top surface of the partition (8); a second Y-direction displacement module (52) is fixedly connected to the top of the support frame (51); a mobile end of the second Y-direction displacement module (52) is fixedly connected to a pipette (53); an X-direction displacement module (57) is arranged below the pipette (53); a mobile end of the X-direction displacement module (57) is fixedly connected to a tip box (54) and a deep-well plate (55); the tip box (54) and the deep-well plate (55) are arranged below the pipette (53); and a tip waste box (56) is fixedly connected to one side of the X-direction displacement module (57).

8. The automated bacterial liquid coating device according to claim 2, characterized in that: The unloading mechanism (6) includes a shaker (61) fixedly connected to the bottom of the lower chamber, the top surface of the shaker (61) is fixedly connected to a second closed-loop stepper motor, the output shaft of the second closed-loop stepper motor is drivingly connected to the center of a support plate (62), and a plurality of second silo monomers (63) for placing culture plates (9) are fixedly connected to the support plate (62).

9. The automated bacterial liquid coating device according to claim 8, characterized in that: A second electric gripper for grabbing a culture plate (9) is disposed above the second silo monomer (63); the second electric gripper is fixedly connected to a second Z-axis lifting module for driving the second electric gripper to rise and fall; the second Z-axis lifting module is fixedly connected to a second X-axis transport module for driving the second Z-axis lifting module to move horizontally; the second electric gripper, the second Z-axis lifting module, and the second X-axis transport module are disposed in the upper cavity; and the second X-axis transport module is fixedly connected to the top surface of the partition (8).

10. The automated bacterial liquid coating equipment according to claim 1, characterized in that: An observation window (71) is embedded in the upper part of one side of the shell (7), a front cabinet door (72) is arranged below the observation window (71), and the front cabinet door (72) is installed on the shell (7), and a plurality of side cabinet doors (73) are installed on the other side of the shell (7).

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