Biological culture device for biological experiment

By designing an automatically supplied and controlled biological experiment biological aquaculture device, the bacterial contamination problem caused by manual operation is solved, automatic culture fluid addition and liquid management are realized in a sterile environment, and the safety and efficiency of biological cell culture are improved.

CN119161980AInactive Publication Date: 2024-12-20WUXI SANGER BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411315098.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-20
Publication Date
2024-12-20
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing containers storing culture medium need to manually open the sterile container after the culture medium is consumed, which can easily lead to bacterial contamination.

Method used

A biological aquaculture device for biological experiments is designed, including protective sleeves, storage containers, on-off components, hoisting components and feedback components. By automatically supplying and controlling the addition of culture fluid, it avoids manual operation to destroy the sterile environment.

Benefits of technology

It realizes automatic addition of culture medium in a sterile environment to prevent bacterial contamination, and ensures correct positioning and liquid management of the container through limiting and feedback mechanisms, improving the safety and efficiency of biological cells culture.

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Abstract

The invention belongs to the technical field of biological culture, and particularly discloses a biological culture device for biological experiments, which comprises a protective sleeve, a base is fixed at the bottom of the protective sleeve, a storage container is arranged between the inner walls of the protective sleeve, and clamping mechanisms for limiting the storage container are arranged on the inner walls of the two sides of the protective sleeve. A fixing column is mounted in the middle of the inner bottom surface of the protective sleeve, a jacking assembly is arranged in the fixing column, and a plurality of on-off assemblies are arranged on the four sides of the fixing column; according to the device, through the arrangement of the on-off assembly, the jacking assembly, the feedback assembly and the clamping mechanism, the storage container can be positioned when the storage container is put into the protective sleeve, meanwhile, the feedback assembly can be triggered to work when the liquid level of a culture solution in the storage container is low, and the culture solution is supplied to the interior of the storage container; and a series of problems caused by manually opening the storage container from a sterile environment and adding the culture solution are avoided.
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Description

Technical Field

[0001] The invention relates to the technical field of biological breeding, and in particular to a biological breeding device for biological experiments. Background Art

[0002] Biological cells are stem cells. Simply put, they are a type of primitive undifferentiated cells with multidirectional differentiation potential and self-replication ability. They are the original cells that form various tissues and organs of mammals. Stem cells have commonalities in morphology, usually round or oval, small cell size, relatively large nucleus, mostly euchromatin, and have high telomerase activity.

[0003] Currently, when culturing biological cells, in order for the biological cells to better absorb nutrients and facilitate the subsequent extraction of the biological cells, the biological cells need to be placed in a culture medium for culture. However, after the culture medium is consumed, the existing container for storing the culture medium needs to be opened and manually added, which makes it easy to cause bacterial contamination when adding the culture medium. Summary of the invention

[0004] The purpose of the present invention is to provide a biological breeding device for biological experiments, which solves the problem that after the culture fluid is consumed, the sterile culture container needs to be opened and manually added, which easily causes bacterial contamination when adding culture fluid.

[0005] To achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a biological breeding device for biological experiments, comprising a protective cover, a base is fixed at the bottom of the protective cover, a storage container is arranged between the inner walls of the protective cover, and a clamping mechanism for limiting the storage container is arranged on the inner walls of both sides of the protective cover, a fixing column is installed at the middle of the inner bottom surface of the protective cover, a lifting component is arranged inside the fixing column, and a plurality of on-off components are arranged on four sides of the fixing column, a docking groove is opened at the bottom of the storage container, and the fixing column is clamped between the inner walls of the docking groove;

[0006] A fixing sleeve is fixed on the inner wall of one side of the storage container near the top edge, and a feedback component is arranged inside the fixing sleeve. A plurality of guide holes penetrating into the docking groove are equidistantly provided on the inner bottom surface of the storage container along the circumferential direction, and limiting openings are provided on the outer surfaces of both sides of the storage container.

[0007] Preferably, the jacking assembly includes a jacking plate, which is arranged on the top of a fixed column, an adjusting cavity is opened inside the fixed column, a jacking rod is arranged inside the adjusting cavity, the top of the jacking rod slides through the top of the fixed column and is fixed to the bottom of the jacking plate, a conical ring is fixed on the outer surface of the jacking rod, the conical ring is located inside the adjusting cavity, a first spring is fixed on the top of the conical ring, and the top of the first spring is fixed to the inner top surface of the adjusting cavity.

[0008] Preferably, the on-off assembly includes a conical block, a receiving cavity is provided on the outer surface of the fixed column, the conical block is located inside the receiving cavity, a reciprocating cavity is provided inside the fixed column on one side of the receiving cavity, a contact rod is provided inside the reciprocating cavity, one end of the contact rod slides through the inside of the receiving cavity and is fixed on one side of the conical block.

[0009] Preferably, the other end of the contact rod slides through the interior of the adjustment cavity and fits into the conical surface of the conical ring. A fixing ring is installed on the outer surface of the contact rod, and a third spring is fixed on one side of the fixing ring. One end of the third spring is fixed on one inner wall of the reciprocating cavity.

[0010] Preferably, a through hole is provided on the outer surface of the contact rod, an annular collecting chamber is provided inside the protective sleeve below the fixed column, a plurality of drainage holes are provided on the inner top surface of the annular collecting chamber at equal intervals along the circumferential direction, the contact rod passes through the drainage holes correspondingly, the plurality of drainage holes all pass through to the top of the fixed column, the through hole and the drainage hole are connected to each other, and the tops of the plurality of drainage holes are relatively connected to the guide holes.

[0011] Preferably, the locking mechanism includes two spring clamps, and side openings are provided on the inner walls of both sides of the protective sleeve near the top edge. The two spring clamps are correspondingly rotatably connected between the inner walls of the side openings, and two side cavities are provided inside the protective sleeve below the annular collecting cavity, and one end of the two side cavities penetrates to the inner bottom surface of the side openings.

[0012] Preferably, side push rods are provided inside the two side cavities, one ends of the two side push rods extend to the inside of the side opening respectively, and fit together with one side of the bottom of the spring clamp, a hexagonal hole is provided on the inner bottom surface of the adjusting cavity, the bottom of the lifting rod is slidably connected between the inner walls of the hexagonal hole, the hexagonal hole is communicated with the two side cavities, and the other ends of the two side push rods are fixed on the outer surface of the lifting rod respectively.

[0013] Preferably, the feedback component includes a floating plate, which is located below the fixed sleeve, a guide plate is fixed on the top of the floating plate, the top of the guide plate slides and passes through the interior of the fixed sleeve, a push block is fixed on the top of the guide plate, and the push block is located inside the fixed sleeve, a limiting cavity is provided on the inner wall of the storage container, an insertion rod is provided inside the limiting cavity, one end of the insertion rod slides and passes through the outer surface of the storage container, one end of the insertion rod is inclined, and the other end of the insertion rod slides and passes through the interior of the fixed sleeve, a triangular block is fixed to the other end of the insertion rod, the inclined surface of the triangular block is in contact with the outer surface of one side of the push block, a limiting ring is fixed on the outer surface of the insertion rod, and the limiting ring is slidably connected between the inner walls of the limiting cavity.

[0014] Preferably, a flow channel is provided on the inner wall of the protective sleeve, the bottom of the flow channel extends to the interior of the annular collecting chamber, the top of the flow channel extends to the outer surface of the protective sleeve, a feed pipe is fixed to the outer surface of the protective sleeve, the feed pipe is communicated with the top of the flow channel, a hexagonal cavity is provided inside the protective sleeve, a through hole is provided on one side of the hexagonal cavity, one end of the through hole passes through the interior of the protective sleeve, the through hole and the flow channel are communicated with each other, and one end of the insertion rod is opposite to one side of the through hole.

[0015] Preferably, a hexagonal plate is slidably connected between the inner walls of the hexagonal cavity, a guide rod is fixed on one side of the hexagonal plate, one end of the guide rod slides through to the outside of the protective sleeve, two copper sheets are fixed on the outer surface of the guide rod, and two notches are provided on the inner wall of the other side of the hexagonal cavity at the connection of the guide rod, copper blocks are provided inside the two notches, and the two copper blocks correspond to and fit with the two copper sheets, a second spring is fixed between one side of the hexagonal plate and the inner wall of one side of the hexagonal cavity, an alarm is provided on the base, and the alarm is electrically connected to the two copper blocks.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The present invention is provided with an on-off component, a lifting component, a feedback component and a locking mechanism, which is conducive to positioning the storage container when the storage container is placed inside the protective cover. At the same time, when the liquid level of the culture solution inside the storage container is low, the feedback component can be triggered to work, and the culture solution can be supplied to the storage container, thereby avoiding a series of problems caused by manually opening the storage container from a sterile environment and adding the culture solution;

[0018] 2. The present invention is provided with an on-off assembly, which can be triggered after the storage container is placed inside the protective cover, so that the culture fluid is supplied to the storage container. At the same time, the on-off assembly will drive the lifting assembly to operate after being triggered. When the on-off assembly is working, the storage container is first placed inside the protective cover. During the sliding process of the storage container, the inner wall of the docking groove and the inclined surface of the conical block contact each other, thereby pushing the conical block into the storage cavity, thereby driving the contact rod to move so that the through hole and the drainage hole are connected to each other;

[0019] 3. The present invention is provided with a lifting assembly and a locking mechanism, so that the storage container can be limited and fixed after the storage container is placed inside the protective cover. When the lifting assembly is working, when the contact rod moves, one end of the contact rod fits with the inclined surface of the conical ring, so the conical ring is lifted upward, and then the lifting rod is driven to lift upward, so that the lifting plate lifts the storage container upward. When the locking mechanism is working, the lifting rod drives the side push rod to slide upward during the lifting process. During the upward sliding process of the side push rod, the top side fits with one side of the spring clamping plate, so that one side of the spring clamping plate can be pushed into the protective cover, so that it is locked inside the limiting opening on the storage container, and at the same time, the upward lifting force of the lifting plate is cooperated, so that the storage container can be locked in position.

[0020] 4. The present invention is provided with a feedback component, which can cut off the flow channel and stop injecting the culture solution into the storage container when the culture solution inside the storage container is injected to a certain height. At the same time, when the liquid level of the culture solution inside the storage container is low, the feedback component is triggered to open the flow channel to inject the culture solution into the storage container. When the feedback component is working, when the liquid level of the culture solution inside the storage container rises to a certain height, the float plate will be pushed upward, and during the upward sliding process of the float plate, one end of the insertion rod will be pushed to slide into the through hole, thereby cutting off the flow channel. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention provides a schematic diagram of the main stereoscopic structure of a biological breeding device for biological experiments;

[0022] Figure 2 The present invention provides a top-view stereoscopic structural diagram of a protective cover in a biological breeding device for biological experiments;

[0023] Figure 3 The present invention provides a schematic diagram of a three-dimensional structure of a storage container in a biological breeding device for biological experiments from one side;

[0024] Figure 4 The present invention provides a schematic diagram of a three-dimensional structure of a storage container in a biological breeding device for biological experiments from another side;

[0025] Figure 5The present invention provides a schematic diagram of a three-dimensional structure of a protective cover in a biological breeding device for biological experiments;

[0026] Figure 6 The present invention provides a schematic diagram of the other side cross-sectional stereoscopic structure of a protective cover in a biological breeding device for biological experiments;

[0027] Figure 7 For the present invention Figure 4 A partial enlarged view of point A in the middle;

[0028] Figure 8 For the present invention Figure 5 A partial enlarged view of point B in the middle;

[0029] Fig. 9 For the present invention Figure 6 A magnified partial view of point C in the middle.

[0030] In the figure: 1, protective cover; 2, storage container; 3, base; 4, alarm; 5, feed pipe; 6, fixed column; 7, lifting plate; 8, limit opening; 9, docking groove; 10, guide hole; 11, fixed cover; 12, floating plate; 13, plug rod; 14, limit cavity; 15, triangular block; 16, push block; 17, guide plate; 18, limit ring; 19, flow channel; 20, annular collecting cavity; 21, regulating cavity; 22, lifting rod; 23, conical ring ; 24. First spring; 25. Hexagonal hole; 26. Hexagonal cavity; 27. Guide rod; 28. Through hole; 29. ​​Second spring; 30. Notch; 31. Copper block; 32. Copper sheet; 33. Hexagonal plate; 34. Side cavity; 35. Side push rod; 36. Side port; 37. Spring clamp; 38. Reciprocating cavity; 39. Contact rod; 40. Drainage hole; 41. Storage cavity; 42. Conical block; 43. Through hole; 44. Fixing ring; 45. Third spring. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] See also Figure 1-9The present invention provides a technical solution: a biological breeding device for biological experiments, comprising a protective cover 1, a base 3 is fixed at the bottom of the protective cover 1, a storage container 2 is arranged between the inner walls of the protective cover 1, and both inner walls of the protective cover 1 are provided with a clamping mechanism for limiting the storage container 2, a fixed column 6 is installed at the middle of the inner bottom surface of the protective cover 1, a lifting component is arranged inside the fixed column 6, and a plurality of on-off components are arranged on the four sides of the fixed column 6, a docking groove 9 is opened at the bottom of the storage container 2, and the fixed column 6 is clamped between the inner walls of the docking groove 9;

[0033] A fixing sleeve 11 is fixed to the inner wall of one side of the storage container 2 near the top edge, and a feedback component is arranged inside the fixing sleeve 11. A plurality of guide holes 10 penetrating into the docking groove 9 are equidistantly provided on the inner bottom surface of the storage container 2 along the circumferential direction, and limiting openings 8 are provided on the outer surfaces of both sides of the storage container 2.

[0034] The effect achieved is that, by providing an on-off component, it can be triggered after the storage container 2 is placed inside the protective cover 1, so that the culture solution is supplied to the storage container 2. At the same time, the on-off component will also drive the lifting component to move after being triggered. By providing the lifting component and the locking mechanism, the storage container 2 can be limited and fixed after being placed inside the protective cover 1. By providing the feedback component, when the culture solution in the storage container 2 is injected to a certain height, the flow channel 19 can be cut off to stop injecting the culture solution into the storage container 2. At the same time, when the liquid level of the culture solution in the storage container 2 is low, the feedback component is triggered to make the flow channel 19 open to inject the culture solution into the storage container 2, thereby avoiding a series of problems caused by manually opening the storage container 2 from a sterile environment and adding the culture solution.

[0035] like Figure 3 , Figure 5 , Figure 6 and Fig. 9As shown, the switch assembly includes a conical block 42, a receiving cavity 41 is provided on the outer surface of the fixed column 6, the conical block 42 is located inside the receiving cavity 41, and a reciprocating cavity 38 is provided inside the fixed column 6 on one side of the receiving cavity 41. A contact rod 39 is provided inside the reciprocating cavity 38, one end of the contact rod 39 slides through the inside of the receiving cavity 41 and is fixed on one side of the conical block 42, and the other end of the contact rod 39 slides through the inside of the adjustment cavity 21 and fits with the conical surface of the conical ring 23, and a fixing ring 44 is installed on the outer surface of the contact rod 39, and the fixing ring A third spring 45 is fixed on one side of 44, and one end of the third spring 45 is fixed on an inner wall of one side of the reciprocating cavity 38. A through hole 43 is provided on the outer surface of the contact rod 39, and an annular collecting cavity 20 is provided inside the protective sleeve 1 below the fixed column 6. A plurality of drainage holes 40 are provided on the inner top surface of the annular collecting cavity 20 at equal intervals along the circumferential direction. The contact rod 39 passes through the drainage hole 40 correspondingly, and the plurality of drainage holes 40 all pass through the top of the fixed column 6. The through hole 43 is connected to the drainage hole 40, and the tops of the plurality of drainage holes 40 are relatively connected to the guide hole 10.

[0036] The effect achieved is that the feed pipe 5 is connected to the external culture fluid supply pipeline. After the storage container 2 is placed in the protective sleeve 1, the culture fluid is supplied to the inside of the annular collecting chamber 20 through the feed pipe 5. During the sliding down process of the storage container 2, the inner wall of the docking groove 9 contacts the inclined surface of the conical block 42, thereby pushing the conical block 42 into the storage chamber 41, thereby driving the contact rod 39 to move so that the through hole 43 and the drainage hole 40 are connected to each other. At the same time, when the fixed column 6 completely slides into the docking groove 9, the diversion hole 10 and the drainage hole 40 are connected to each other. At this time, the culture fluid flows from the drainage hole 40 on the top surface of the annular collecting chamber 20 into the diversion hole 10, and finally flows into the storage container 2. At this time, the first spring 24 and the third spring 45 are both in a compressed state.

[0037] like Figure 2 , Figure 5 and Fig. 9 As shown, the jacking assembly includes a jacking plate 7, which is arranged on the top of the fixed column 6. An adjusting cavity 21 is opened inside the fixed column 6. A jacking rod 22 is arranged inside the adjusting cavity 21. The top of the jacking rod 22 slides through the top of the fixed column 6 and is fixed to the bottom of the jacking plate 7. A conical ring 23 is fixed on the outer surface of the jacking rod 22. The conical ring 23 is located inside the adjusting cavity 21. A first spring 24 is fixed on the top of the conical ring 23. The top of the first spring 24 is fixed to the inner top surface of the adjusting cavity 21.

[0038] The effect achieved is that when the contact rod 39 moves, one end of the contact rod 39 fits against the inclined surface of the conical ring 23, so the conical ring 23 is lifted upward, thereby driving the lifting rod 22 to lift upward, so that the lifting plate 7 lifts the storage container 2 upward.

[0039] like Figure 3 , Figure 5 and Figure 6 As shown, the locking mechanism includes two spring clamps 37, and side openings 36 are provided on the inner walls of both sides of the protective sleeve 1 near the top edge. The two spring clamps 37 are correspondingly rotatably connected between the inner walls of the side openings 36. The interior of the protective sleeve 1 is located below the annular collecting chamber 20 and has two side chambers 34. One end of the two side chambers 34 penetrates to the inner bottom surface of the side opening 36. Side push rods 35 are provided inside the two side chambers 34. One end of the two side push rods 35 extends to the inside of the side opening 36 and fits with one side of the bottom of the spring clamps 37. A hexagonal hole 25 is provided on the inner bottom surface of the adjusting chamber 21. The bottom of the lifting rod 22 is slidably connected between the inner walls of the hexagonal hole 25. The hexagonal hole 25 is communicated with the two side chambers 34, and the other ends of the two side push rods 35 are correspondingly fixed on the outer surface of the lifting rod 22.

[0040] The effect achieved is that, as the lifting rod 22 is lifted upward, the side push rod 35 is driven to slide upward. During the upward sliding process of the side push rod 35, the top side fits with the side of the spring clamp 37, so that the side of the spring clamp 37 can be pushed toward the inside of the protective sleeve 1, so that it is engaged in the limit opening 8 on the storage container 2, and at the same time, cooperates with the upward lifting force of the lifting plate 7, so that the storage container 2 can be limited and locked.

[0041] like Figure 1 , Figure 4 , Figure 5 , Figure 7 and Figure 8As shown, the feedback assembly includes a floating plate 12, the floating plate 12 is located below the fixed sleeve 11, a guide plate 17 is fixed on the top of the floating plate 12, the top of the guide plate 17 slides through the inside of the fixed sleeve 11, a push block 16 is fixed on the top of the guide plate 17, the push block 16 is located inside the fixed sleeve 11, a limited cavity 14 is provided on the inner wall of the storage container 2, an insertion rod 13 is arranged inside the limited cavity 14, one end of the insertion rod 13 slides through the outer surface of the storage container 2, and one end of the insertion rod 13 The other end of the plug rod 13 slides through the interior of the fixed sleeve 11, and a triangular block 15 is fixed to the other end of the plug rod 13. The inclined surface of the triangular block 15 fits with the outer surface of one side of the push block 16. A limiting ring 18 is fixed to the outer surface of the plug rod 13. The limiting ring 18 is slidably connected between the inner walls of the limiting cavity 14. A flow channel 19 is opened on the inner wall of the protective sleeve 1. The bottom of the flow channel 19 extends to the inside of the annular collecting cavity 20, and the top of the flow channel 19 extends to the outer surface of the protective sleeve 1 The outer surface of the protective sleeve 1 is fixed with a feed pipe 5, and the feed pipe 5 is connected with the top of the flow channel 19. The interior of the protective sleeve 1 is provided with a hexagonal cavity 26, and a through hole 28 is provided on one side of the hexagonal cavity 26. One end of the through hole 28 penetrates into the interior of the protective sleeve 1, and the through hole 28 is connected with the flow channel 19. One end of the insertion rod 13 is opposite to one side of the through hole 28. A hexagonal plate 33 is slidably connected between the inner walls of the hexagonal cavity 26, and a guide rod 27 is fixed on one side of the hexagonal plate 33. One end of the guide rod 27 is fixed to the inner wall of the hexagonal cavity 26. The end slides through the outside of the protective sleeve 1, and two copper sheets 32 are fixed on the outer surface of the guide rod 27. Two notches 30 are provided on the inner wall of the other side of the hexagonal cavity 26 at the connection of the guide rod 27. Copper blocks 31 are provided inside the two notches 30. The two copper blocks 31 correspond to the two copper sheets 32 and fit each other. A second spring 29 is fixed between one side of the hexagonal plate 33 and the inner wall of one side of the hexagonal cavity 26. An alarm 4 is provided on the base 3, and the alarm 4 is electrically connected to the two copper blocks 31.

[0042] The effect achieved is that, in the process of injecting culture solution into the storage container 2, when the liquid level of the culture solution in the storage container 2 rises to a certain height, the floating plate 12 will be pushed upward, and the push block 16 will be driven to move upward during the upward sliding of the floating plate 12. During the upward movement of the push block 16, one side of the push block 16 fits with the inclined surface of the triangular block 15, so the triangular block 15 will be pushed to one side, and then one end of the insertion rod 13 will be pushed to slide into the through hole 28. After one end of the insertion rod 13 completely enters the flow channel 19, the flow channel 19 can be cut off. At the same time, when one end of the insertion rod 13 enters the flow channel 19, the hexagonal The plate 33 is pushed outward, and during the pushing process, the copper block 31 and the copper sheet 32 ​​are fitted together, thereby turning on the alarm 4 and generating an alarm, reminding people that the culture solution inside the storage container 2 has been filled. When the liquid level of the culture solution inside the storage container 2 drops, the upward buoyancy of the float plate 12 is reduced, and the second spring 29 is reset at this time, pushing the rod 13 to the other side until the inclined surface on the rod 13 slides into the interior of the flow channel 19. Since there is always culture solution flowing downward inside the flow channel 19, when the thrust of the downward flow of the culture solution acts on the inclined surface of the rod 13, the rod 13 can be pushed in the opposite direction, thereby making the flow channel 19 conductive.

[0043] Working principle: When using the device, the feed pipe 5 is connected to the external culture fluid supply pipeline. After the storage container 2 is placed inside the protective sleeve 1, the culture fluid is supplied to the inside of the annular collecting chamber 20 through the feed pipe 5. During the sliding process of the storage container 2, the inner wall of the docking groove 9 contacts the inclined surface of the conical block 42, thereby pushing the conical block 42 into the storage chamber 41, thereby driving the contact rod 39 to move so that the through hole 43 and the drainage hole 40 are connected to each other. At the same time, when the fixing column 6 completely slides into the docking groove 9, The guide hole 10 is connected with the drainage hole 40, and the culture fluid flows from the drainage hole 40 on the top surface of the annular collecting chamber 20 into the guide hole 10, and finally flows into the storage container 2. When the contact rod 39 moves, one end of the contact rod 39 fits with the inclined surface of the conical ring 23, so the conical ring 23 is lifted upward, and then the lifting rod 22 is driven to lift upward, so that the lifting plate 7 lifts the storage container 2 upward. Since the lifting rod 22 is driven to slide upward during the upward lifting process, the side push rod 35 is driven to slide upward during the upward sliding process of the side push rod 35. By fitting one side of the top with one side of the spring clamp 37, the one side of the spring clamp 37 can be pushed into the protective sleeve 1 so that it is engaged in the limit opening 8 on the storage container 2. At the same time, with the upward lifting force of the lifting plate 7, the storage container 2 can be limited and locked. In the process of injecting culture solution into the storage container 2, when the liquid level of the culture solution in the storage container 2 rises to a certain height, the floating plate 12 will be pushed upward. In the process of the floating plate 12 sliding upward, the push block 16 will be driven to move upward. The upward movement of the push block 16 During the process, since one side of the push block 16 fits with the inclined surface of the triangular block 15, the triangular block 15 will be pushed to one side, and then the one end of the plug rod 13 will be pushed to slide into the through hole 28. After one end of the plug rod 13 completely enters the flow channel 19, the flow channel 19 can be cut off. At the same time, when one end of the plug rod 13 enters the flow channel 19, the hexagonal plate 33 will be pushed outward. During the pushing process, the copper block 31 and the copper sheet 32 ​​fit each other, and then the alarm 4 is turned on to generate an alarm, reminding people that the culture solution inside the storage container 2 is full.

[0044] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A biological breeding device for biological experiments, characterized in that: The protective sleeve (1) comprises a protective sleeve (1), a base (3) is fixed at the bottom of the protective sleeve (1), a storage container (2) is arranged between the inner walls of the protective sleeve (1), and the inner walls on both sides of the protective sleeve (1) are provided with a snap-fit ​​mechanism for limiting the storage container (2), a fixing column (6) is installed at the middle of the inner bottom surface of the protective sleeve (1), a lifting component is arranged inside the fixing column (6), and a plurality of switch components are arranged on four sides of the fixing column (6), a docking groove (9) is opened at the bottom of the storage container (2), and the fixing column (6) is snap-fitted between the inner walls of the docking groove (9); A fixing sleeve (11) is fixed to the inner wall of one side of the storage container (2) near the top edge, and a feedback component is arranged inside the fixing sleeve (11). The inner bottom surface of the storage container (2) is provided with a plurality of guide holes (10) that penetrate into the docking groove (9) at equal intervals along the circumferential direction, and the outer surfaces of both sides of the storage container (2) are provided with limiting openings (8).

2. The biological breeding device for biological experiments according to claim 1, characterized in that: The lifting assembly comprises a lifting plate (7), wherein the lifting plate (7) is arranged at the top of a fixed column (6), an adjusting cavity (21) is provided inside the fixed column (6), a lifting rod (22) is provided inside the adjusting cavity (21), the top of the lifting rod (22) slides through the top of the fixed column (6) and is fixed to the bottom of the lifting plate (7), a conical ring (23) is fixed to the outer surface of the lifting rod (22), the conical ring (23) is located inside the adjusting cavity (21), a first spring (24) is fixed to the top of the conical ring (23), and the top of the first spring (24) is fixed to the inner top surface of the adjusting cavity (21).

3. The biological breeding device for biological experiments according to claim 2, characterized in that: The switch assembly comprises a conical block (42), a receiving cavity (41) is provided on the outer surface of the fixed column (6), the conical block (42) is located inside the receiving cavity (41), a reciprocating cavity (38) is provided inside the fixed column (6) on one side of the receiving cavity (41), a contact rod (39) is provided inside the reciprocating cavity (38), one end of the contact rod (39) slides through the inside of the receiving cavity (41) and is fixed on one side of the conical block (42).

4. The biological breeding device for biological experiments according to claim 3, characterized in that: The other end of the contact rod (39) slides through the interior of the adjustment cavity (21) and fits with the conical surface of the conical ring (23). A fixing ring (44) is installed on the outer surface of the contact rod (39). A third spring (45) is fixed on one side of the fixing ring (44). One end of the third spring (45) is fixed on one side inner wall of the reciprocating cavity (38).

5. The biological breeding device for biological experiments according to claim 4, characterized in that: The outer surface of the contact rod (39) is provided with a through hole (43), and the interior of the protective sleeve (1) is provided with an annular collecting chamber (20) located below the fixed column (6). The inner top surface of the annular collecting chamber (20) is provided with a plurality of drainage holes (40) equidistantly arranged along the circumferential direction. The contact rod (39) passes through the drainage holes (40) correspondingly, and the plurality of drainage holes (40) all pass through the top of the fixed column (6). The through hole (43) is connected to the drainage hole (40), and the tops of the plurality of drainage holes (40) are relatively connected to the guide hole (10).

6. The biological breeding device for biological experiments according to claim 5, characterized in that: The locking mechanism comprises two spring clamps (37), and side openings (36) are provided on the inner walls of both sides of the protective sleeve (1) near the top edge. The two spring clamps (37) are correspondingly rotatably connected between the inner walls of the side openings (36), and the interior of the protective sleeve (1) is provided with two side cavities (34) located below the annular collecting cavity (20), and one end of the two side cavities (34) penetrates to the inner bottom surface of the side opening (36).

7. The biological breeding device for biological experiments according to claim 6, characterized in that: The two side cavities (34) are each provided with a side push rod (35), one end of each of the two side push rods (35) correspondingly extends to the inside of the side opening (36) and fits with one side of the bottom of the spring clamp (37), the inner bottom surface of the adjustment cavity (21) is provided with a hexagonal hole (25), the bottom of the lifting rod (22) is slidably connected between the inner walls of the hexagonal hole (25), the hexagonal hole (25) and the two side cavities (34) are communicated with each other, and the other ends of the two side push rods (35) are correspondingly fixed on the outer surface of the lifting rod (22).

8. The biological breeding device for biological experiments according to claim 7, characterized in that: The feedback component comprises a floating plate (12), wherein the floating plate (12) is located below the fixed sleeve (11), a guide plate (17) is fixed on the top of the floating plate (12), the top of the guide plate (17) slides through the inside of the fixed sleeve (11), a push block (16) is fixed on the top of the guide plate (17), and the push block (16) is located inside the fixed sleeve (11), a limiting cavity (14) is provided on the inner wall of the storage container (2), and an insertion rod (13) is provided inside the limiting cavity (14), One end of the insertion rod (13) slides through the outer surface of the storage container (2), one end of the insertion rod (13) is inclined, and the other end of the insertion rod (13) slides through the inside of the fixed sleeve (11). A triangular block (15) is fixed to the other end of the insertion rod (13), and the inclined surface of the triangular block (15) is in contact with the outer surface of one side of the push block (16). A limiting ring (18) is fixed to the outer surface of the insertion rod (13), and the limiting ring (18) is slidably connected between the inner walls of the limiting cavity (14).

9. The biological breeding device for biological experiments according to claim 8, characterized in that: The inner wall of the protective sleeve (1) is provided with a flow channel (19), the bottom of the flow channel (19) extends to the interior of the annular collecting chamber (20), the top of the flow channel (19) extends to the outer surface of the protective sleeve (1), a feed pipe (5) is fixed to the outer surface of the protective sleeve (1), the feed pipe (5) and the top of the flow channel (19) are communicated with each other, a hexagonal cavity (26) is provided inside the protective sleeve (1), a through hole (28) is provided on one side of the hexagonal cavity (26), one end of the through hole (28) passes through the interior of the protective sleeve (1), the through hole (28) and the flow channel (19) are communicated with each other, and one end of the insertion rod (13) is opposite to one side of the through hole (28).

10. The biological breeding device for biological experiments according to claim 9, characterized in that: A hexagonal plate (33) is slidably connected between the inner walls of the hexagonal cavity (26); a guide rod (27) is fixed to one side of the hexagonal plate (33); one end of the guide rod (27) slides through the outside of the protective sleeve (1); two copper sheets (32) are fixed to the outer surface of the guide rod (27); two notches (30) are provided on the inner wall of the other side of the hexagonal cavity (26) at the connection with the guide rod (27); copper blocks (31) are provided inside the two notches (30); the two copper blocks (31) are correspondingly fitted with the two copper sheets (32); a second spring (29) is fixed between one side of the hexagonal plate (33) and the inner wall of one side of the hexagonal cavity (26); an alarm (4) is provided on the base (3); the alarm (4) is electrically connected to the two copper blocks (31).