Detection sample dilution system and method and automatic dilution machine

Abstract

The invention relates to a detection sample diluting system and method and an automatic diluting machine. The detection sample diluting method comprises the following steps: extracting a stored diluent into a reaction cup; a certain amount of sample is extracted and injected into a reaction cup, the sample and the diluent are mixed and fully shaken up, and a first mixed solution is obtained; extracting a certain amount of the first mixed solution for temporary storage, extracting the remaining first mixed solution from the reaction cup, and then extracting the temporarily stored first mixed solution back into the reaction cup; injecting a certain amount of diluent into the first mixed solution in the reaction cup, then injecting a certain amount of reagent into the reaction cup, and fully shaking up to obtain a second mixed solution, thereby completing the dilution of the sample. Compared with the prior art, the sample solution can be rapidly diluted, the dilution multiple is flexible and adjustable, the sample testing efficiency is improved, and the sample dilution cost can be reduced.

Description

Technical Field

[0001] The present invention relates to the field of medical testing technology, and in particular to a testing sample dilution system and method, and an automatic diluting machine. Background Art

[0002] In the prior art, in the field of in vitro diagnostic analysis instruments and equipment, when samples react with reagents, according to the requirements of the relevant reagents, the concentration of the samples participating in the reaction must be within a certain range in order to obtain reliable test results. In clinical tests, the sample concentration may exceed the required range of the reagents. In this case, the sample needs to be diluted before reacting with the reagents.

[0003] At present, in the field of in vitro diagnostic analysis instruments and equipment, the conventional methods for diluting samples are manual dilution and specific instrument dilution; manual dilution is that the staff uses tools such as a dispenser to dilute the sample manually, which is relatively complicated to operate and is very easy to introduce errors in manual operation, while wasting manpower and low detection efficiency; specific instrument dilution is to dilute the sample using a diluter, which requires the staff to put the sample into the diluter for dilution, take out the sample after the dilution is completed, and then put it into the detection instrument for detection, which is equivalent to adding an intermediate process, increasing the complexity of the detection, reducing the consistency and efficiency of the detection, and also increasing the workload of the staff and the probability of error. Therefore, it is necessary to solve the problems existing in the prior art. Summary of the invention

[0004] The present invention aims to solve at least one of the above-mentioned technical problems in the prior art to a certain extent. To this end, one object of the present invention is to provide a detection sample dilution system and method, and an automatic diluting machine that can achieve rapid dilution of a sample solution, with a flexible and adjustable dilution multiple, improve sample testing efficiency, and reduce sample dilution costs.

[0005] The technical solution of the present invention to solve the above technical problem is as follows: A method for diluting a detection sample comprises the following steps:

[0006] Draw the stored dilution into the reaction cup;

[0007] A certain amount of sample is drawn and injected into the reaction cup, the sample and the diluent are mixed, and the mixture is shaken thoroughly to obtain a first mixed solution;

[0008] Extracting a certain amount of the first mixed solution for temporary storage, extracting the remaining first mixed solution from the reaction cup, and then extracting the temporarily stored first mixed solution back into the reaction cup;

[0009] A certain amount of diluent is injected into the first mixed solution in the reaction cup and shaken thoroughly to obtain a second mixed solution; then a certain amount of reagent is injected into the reaction cup and shaken thoroughly to perform the test.

[0010] The beneficial effects of the present invention are as follows: in the process of diluting the sample, a part of the mixed liquid in the first mixed liquid is first extracted, and then an equal volume of diluent is added to the first mixed liquid, so as to achieve rapid dilution of the sample solution, and the dilution multiple is flexibly adjustable, which is widely applicable to the dilution of various high-concentration samples and improves the sample testing efficiency; in the process of diluting the sample, the sample dilution only needs to be completed in the reaction cup, and no special dilution cup is required, which reduces the sample dilution cost and also improves the sample testing efficiency.

[0011] The technical solution of the present invention to solve the above technical problem is as follows: a detection sample dilution system, comprising:

[0012] A reaction cup, wherein the reaction cup mixes the sample and the diluent, and shakes well to obtain a first mixed solution; the reaction cup also discharges the remaining first mixed solution; the reaction cup mixes the first mixed solution and the diluent, and shakes well to obtain a second mixed solution;

[0013] a first pump body, the first pump body is also connected to the reaction cup; the first pump body extracts the diluent into the reaction cup; the first pump body also extracts a certain amount of the first mixed liquid in the reaction cup for temporary storage; after the reaction cup discharges the remaining first mixed liquid, a certain amount of the first mixed liquid is re-introduced into the reaction cup; the first pump body also injects a certain amount of diluent into the reaction cup;

[0014] The sampling needle extracts a sample and injects it into the reaction cup; the sampling needle extracts a certain amount of reagent and injects it into the reaction cup.

[0015] The beneficial effects of the present invention are as follows: in the process of diluting the sample, a part of the mixed liquid in the first mixed liquid is first extracted, and then an equal volume of diluent is added to the first mixed liquid, so as to achieve rapid dilution of the sample solution, and the dilution multiple is flexibly adjustable, which is widely applicable to the dilution of various high-concentration samples and improves the sample testing efficiency; in the process of diluting the sample, the sample dilution only needs to be completed in the reaction cup, and no special dilution cup is required, which reduces the sample dilution cost and also improves the sample testing efficiency.

[0016] Based on the above technical solution, the present invention can also be improved as follows.

[0017] Furthermore, it also includes a first tank body, the first tank body stores the diluent, and the input end of the first pump body is connected to the first tank body.

[0018] Furthermore, it also includes a first three-way solenoid valve, wherein a first port of the first three-way solenoid valve is connected to the first tank body through a pipeline, a second port of the first three-way solenoid valve is connected to the first pump body through a pipeline, and a third port of the first three-way solenoid valve is connected to the reaction cup through a pipeline.

[0019] Furthermore, it also includes a second three-way solenoid valve arranged on the pipeline between the first three-way solenoid valve and the reaction cup, the first port of the second three-way solenoid valve is connected to the third port of the first three-way solenoid valve through a pipeline, and the second port of the second three-way solenoid valve is connected to the reaction cup through a pipeline.

[0020] The beneficial effect of adopting the above further solution is: by regulating the first pump body, the first three-way solenoid valve and the second three-way solenoid valve, automatic sample dilution is facilitated, manual intervention is reduced, and sample testing efficiency is improved.

[0021] Furthermore, it also includes:

[0022] a second tank body, wherein the second tank body stores a cleaning liquid;

[0023] The second pump body, the input end of the second pump body is connected with the second tank body through a pipeline, and the output end of the second pump body is connected with the third port of the second three-way solenoid valve.

[0024] The beneficial effect of adopting the above further solution is: by regulating the second pump body and the second three-way solenoid valve, the reaction cup can be easily cleaned, the cleaning is convenient, the labor intensity of detection is reduced, and the sample testing efficiency is improved.

[0025] Furthermore, it also includes:

[0026] a third pump body, wherein the input end of the third pump body is connected to the reaction cup, and the third pump body pumps out the remaining first mixed liquid in the reaction cup;

[0027] A waste liquid barrel is communicated with the output end of the third pump body, and the waste liquid barrel stores the first mixed liquid extracted by the third pump body.

[0028] The beneficial effect of adopting the above further solution is: by regulating the third pump body, it is convenient to suck the waste liquid from the reaction cup, thereby reducing labor intensity and improving sample testing efficiency.

[0029] Furthermore, it also includes:

[0030] a first solenoid valve, wherein the first solenoid valve is disposed on a pipeline between the first three-way solenoid valve and the second three-way solenoid valve, one port of the first solenoid valve is connected to the third port of the first three-way solenoid valve through a pipeline, and the other port of the first solenoid valve is connected to the first port of the second three-way solenoid valve through a pipeline;

[0031] The second solenoid valve is placed on the pipeline between the reaction cup and the third pump body, one port of the first solenoid valve is connected to the reaction cup through the pipeline, and the other port of the second solenoid valve is connected to the third pump body through the pipeline.

[0032] The beneficial effect of adopting the above further solution is that the first solenoid valve and the second solenoid valve are used to facilitate sample detection and subsequent processing.

[0033] The technical solution of the present invention to solve the above technical problems is as follows: an automatic diluting machine, comprising a test sample dilution system, a host computer and a slave computer

[0034] A host computer, wherein the host computer is used to input a multiple of dilution required and generate dilution information;

[0035] A lower computer, wherein the signal input end of the lower computer is connected to the signal output end of the upper computer, and the output end of the lower computer is connected to the detection sample dilution system; the lower computer receives dilution information, and controls the detection sample dilution system to automatically dilute the detection sample according to the dilution information.

[0036] The beneficial effects of the present invention are as follows: by cooperating with the upper computer and the lower computer to detect the sample dilution system, the sample dilution process is simplified, the test efficiency is improved, and the labor cost and other instrument costs are saved. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 A flow chart of a method for diluting a detection sample according to the present invention;

[0038] Figure 2 This is a schematic diagram of a detection sample dilution system of the present invention;

[0039] Figure 3 The module block diagram of an automatic diluting machine of the present invention is shown in FIG.

[0040] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0041] 1. Detection sample dilution system, 101. reaction cup, 102. first pump body, 103. sampling needle, 104. first tank body, 105. first three-way solenoid valve, 106. second three-way solenoid valve, 107. second tank body, 108. second pump body, 109. third pump body, 110. waste liquid barrel, 111. first solenoid valve, 112. second solenoid valve;

[0042] 2. Upper computer, 3. Lower computer. DETAILED DESCRIPTION

[0043] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0044] like Figure 1 The flowchart of the detection sample dilution method of the present invention is shown in FIG. A detection sample dilution method of the present invention comprises the following steps:

[0045] Extracting the stored diluent into the reaction cup 101;

[0046] A certain amount of sample is drawn and injected into the reaction cup 101, the sample and the diluent are mixed, and the mixture is shaken thoroughly to obtain a first mixed solution;

[0047] A certain amount of the first mixed solution is extracted for temporary storage, the remaining first mixed solution is extracted from the reaction cup 101, and the temporarily stored first mixed solution is extracted back into the reaction cup 101;

[0048] A certain amount of diluent is injected into the first mixed solution in the reaction cup 101 and shaken well to obtain a second mixed solution; a certain amount of reagent is then injected into the reaction cup 101 and shaken well to perform the test.

[0049] In actual application, when testing a sample, the concentration of the sample is first diluted, and a dilution multiple needs to be set first. A certain amount of the sample is mixed with the diluent in the reaction cup 101 to obtain a first mixed solution; subsequently, a certain amount of the first mixed solution is mixed with a certain amount of the diluent, wherein the certain amount of the diluent is consistent with the volume of the extracted first mixed solution, so that the volume of the original first mixed solution is consistent with the volume of the second mixed solution, thereby achieving dilution of the sample.

[0050] In this embodiment, during the sample dilution process, part of the mixed liquid in the first mixed liquid is first extracted, and then an equal volume of diluent is added to the first mixed liquid, thereby achieving rapid dilution of the sample solution. The dilution multiple is flexibly adjustable and is widely applicable to the dilution of various high-concentration samples, thereby improving sample testing efficiency. During the sample dilution process, the sample dilution only needs to be completed in the reaction cup 101, without the need for a specific dilution cup, thereby reducing the sample dilution cost and improving the sample testing efficiency.

[0051] like Figure 2 The schematic diagram of the detection sample dilution system of the present invention is shown in FIG. A detection sample dilution system of the present invention comprises:

[0052] A reaction cup 101, wherein the reaction cup 101 mixes the sample and the diluent, and shakes well to obtain a first mixed solution; the reaction cup 101 also discharges the remaining first mixed solution; the reaction cup 101 mixes the first mixed solution and the diluent, and shakes well to obtain a second mixed solution;

[0053] The first pump body 102 is also connected to the reaction cup 101; the first pump body 102 extracts the diluent into the reaction cup 101; the first pump body 102 also extracts a certain amount of the first mixed liquid in the reaction cup 101 for temporary storage; after the reaction cup 101 discharges the remaining first mixed liquid, a certain amount of the first mixed liquid is re-introduced into the reaction cup 101; the first pump body 102 also injects a certain amount of diluent into the reaction cup 101;

[0054] The sampling needle 103 extracts a sample and injects it into the reaction cup 101 ; the sampling needle 103 extracts a certain amount of reagent and injects it into the reaction cup 101 .

[0055] In actual application, the first pump body 102 is specifically a plunger pump. The first pump body 102 is first used to draw the diluent into the reaction cup 101, and the sampling needle 103 draws the sample and injects it into the reaction cup 101, so that the sample is mixed with the diluent to obtain a first mixed liquid. The first pump body 102 also draws a certain amount of the first mixed liquid in the reaction cup 101 for temporary storage. When the reaction cup 101 also discharges the remaining first mixed liquid, the reaction cup 101 discharges the remaining first mixed liquid, and the first pump body 102 re-introduces a certain amount of the first mixed liquid into the reaction cup 101, and then the first pump body 102 also injects a certain amount of diluent into the reaction cup 101; the reaction cup 101 mixes the first mixed liquid and the diluent, and shakes them thoroughly to obtain a second mixed liquid, completing the dilution of the sample, and then injects a certain amount of reagent into the reaction cup 101, and shakes them thoroughly for testing.

[0056] In this embodiment, during the sample dilution process, part of the mixed liquid in the first mixed liquid is first extracted, and then an equal volume of diluent is added to the first mixed liquid, thereby achieving rapid dilution of the sample solution. The dilution multiple is flexibly adjustable and is widely applicable to the dilution of various high-concentration samples, thereby improving sample testing efficiency. During the sample dilution process, the sample dilution only needs to be completed in the reaction cup, without the need for a specific dilution cup, thereby reducing the sample dilution cost and improving the sample testing efficiency.

[0057] Furthermore, the first tank 104 is included. The first tank 104 stores the diluent, and the input end of the first pump 102 is connected to the first tank 104 .

[0058] It also includes a first three-way solenoid valve 105, a first port of the first three-way solenoid valve 105 is connected to the first tank body 104 through a pipeline, a second port of the first three-way solenoid valve 105 is connected to the first pump body 102 through a pipeline, and a third port of the first three-way solenoid valve 105 is connected to the reaction cup 101 through a pipeline.

[0059] It also includes a second three-way solenoid valve 106 arranged on the pipeline between the first three-way solenoid valve 105 and the reaction cup 101, the first port of the second three-way solenoid valve 106 is connected to the third port of the first three-way solenoid valve 105 through a pipeline, and the second port of the second three-way solenoid valve 106 is connected to the reaction cup 101 through a pipeline.

[0060] In actual application, the first pump body 102 is connected to the first tank body 104 and the reaction cup 101 respectively through the first three-way solenoid valve 105 and the second three-way solenoid valve 106;

[0061] Specifically, the first port of the first three-way solenoid valve 105 is connected to its second port, and the first pump body 102 extracts the diluent in the first tank body 104 through the first three-way solenoid valve 105; then the second port of the first three-way solenoid valve 105 is connected to its third port, and the first port of the second three-way solenoid valve 106 is connected to its second port, and the diluent is transported to the reaction cup 101 through the third port of the first three-way solenoid valve 105, the first port of the second three-way solenoid valve 106, and the second port of the second three-way solenoid valve 106; similarly, when the first pump body 102 extracts the first mixed liquid in the reaction cup 101, the first pump body 102 extracts the first mixed liquid of the reaction cup 101 into the pipeline between the second three-way solenoid valve 106 and the reaction cup 101 through the first three-way solenoid valve 105 and the second three-way solenoid valve 106 for temporary storage, and subsequently returns the first mixed liquid in the pipeline to the reaction cup 101.

[0062] In this embodiment, automatic sample dilution is facilitated by regulating the first pump body 102 , the first three-way solenoid valve 105 , and the second three-way solenoid valve 106 , thereby reducing manual intervention and improving sample testing efficiency.

[0063] Furthermore, it also includes:

[0064] A second tank 107, wherein the second tank 107 stores cleaning liquid;

[0065] The second pump body 108 , the input end of the second pump body 108 is connected to the second tank body 107 through a pipeline, and the output end of the second pump body 108 is connected to the third port of the second three-way solenoid valve 106 .

[0066] In actual application, after the sample test, various detection instruments such as the reaction cup 101 need to be cleaned. The third port of the second three-way solenoid valve 106 is connected to its first port, and the second pump body 108 extracts the cleaning liquid in the second tank body 107 and transports the cleaning liquid to the reaction cup 101 through the second three-way solenoid valve 106.

[0067] In this embodiment, the reaction cup 101 is cleaned by regulating the second pump body 108 and the second three-way solenoid valve 106 , which makes cleaning easier, reduces the labor intensity of detection, and improves sample testing efficiency.

[0068] Furthermore, it also includes:

[0069] A third pump body 109, wherein the input end of the third pump body 109 is connected to the reaction cup 101, and the third pump body 109 pumps out the remaining first mixed liquid in the reaction cup 101;

[0070] The waste liquid barrel 110 is connected to the output end of the third pump body 109 , and the waste liquid barrel 110 stores the first mixed liquid pumped out by the third pump body 109 .

[0071] In actual application, the third pump body 109 extracts the remaining first mixed liquid in the reaction cup 101 and transports it to the waste liquid barrel 110. The third pump body 109 can also suck the waste liquid generated after the reaction cup 101 is tested and cleaned into the waste liquid barrel 110.

[0072] In this embodiment, the third pump body 109 is regulated to facilitate the suction of waste liquid from the reaction cup 101, thereby reducing labor intensity and improving sample testing efficiency.

[0073] Furthermore, it also includes:

[0074] A first solenoid valve 111, wherein the first solenoid valve 111 is disposed on a pipeline between the first three-way solenoid valve 105 and the second three-way solenoid valve 106, wherein one port of the first solenoid valve 111 is connected to a third port of the first three-way solenoid valve 105 through a pipeline, and another port of the first solenoid valve 111 is connected to a first port of the second three-way solenoid valve 106 through a pipeline;

[0075] The second solenoid valve 112 is placed on the pipeline between the reaction cup 101 and the third pump body 109, one port of the first solenoid valve 111 is connected to the reaction cup 101 through a pipeline, and the other port of the second solenoid valve 112 is connected to the third pump body 109 through a pipeline.

[0076] In this embodiment, the first solenoid valve 111 is used to facilitate the regulation of the conduction or blockage of the pipeline between the first three-way solenoid valve 105 and the second three-way solenoid valve 106, and the second solenoid valve 112 is used to facilitate the regulation of the conduction or blockage of the pipeline between the reaction cup 101 and the third pump body 109, so as to facilitate sample detection and subsequent processing.

[0077] Finally, to match the above test sample dilution system, such as Figure 3As shown, the present invention also provides an automatic diluting machine, including a test sample dilution system 1, a host computer 2 and a slave computer 3

[0078] Host computer 2, the host computer 2 is used to input the multiple of dilution required and generate dilution information;

[0079] The lower computer 3 has a signal input end connected to a signal output end of the upper computer 2, and an output end of the lower computer 3 connected to the detection sample dilution system 1; the lower computer 3 receives dilution information, and controls the detection sample dilution system 1 to automatically dilute the detection sample according to the dilution information.

[0080] In the actual application process, the lower computer 3 is electrically connected to the first pump body 102, the first three-way solenoid valve 105, the second three-way solenoid valve 106, the second pump body 108, the third pump body 109, the first solenoid valve 111 and the second solenoid valve 112 respectively, and controls the coordinated operation of the first pump body 102, the first three-way solenoid valve 105, the second three-way solenoid valve 106, the second pump body 108, the third pump body 109, the first solenoid valve 111 and the second solenoid valve 112;

[0081] Before the sample test begins, the tester inputs the required dilution multiple into the upper computer 2. During the dilution process, the lower computer 3 operates the first pump body 102, the first three-way solenoid valve 105, the second three-way solenoid valve 106, the second pump body 108, the third pump body 109, the first solenoid valve 111 and the second solenoid valve 112 to control the amount of the first mixed liquid extracted and the amount of the diluent added, and dilutes the sample according to the set dilution multiple to achieve the target dilution multiple.

[0082] In this embodiment, the upper computer 2 and the lower computer 3 cooperate with the detection sample dilution system 1, which simplifies the sample dilution process, improves the test efficiency, and saves labor costs and other instrument costs.

[0083] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for diluting a test sample, characterized in that: The following steps are involved: Draw the stored dilution into the reaction cup; A certain amount of sample is drawn and injected into the reaction cup, the sample and the diluent are mixed, and the mixture is shaken thoroughly to obtain a first mixed solution; Extracting a certain amount of the first mixed solution for temporary storage, extracting the remaining first mixed solution from the reaction cup, and then extracting the temporarily stored first mixed solution back into the reaction cup; A certain amount of diluent is injected into the first mixed solution in the reaction cup and shaken thoroughly to obtain a second mixed solution; then a certain amount of reagent is injected into the reaction cup and shaken thoroughly to perform the test.

2. A detection sample dilution system, characterized in that: include: A reaction cup, wherein the reaction cup mixes the sample and the diluent, and shakes well to obtain a first mixed solution; the reaction cup also discharges the remaining first mixed solution; the reaction cup mixes the first mixed solution and the diluent, and shakes well to obtain a second mixed solution; a first pump body, the first pump body is also connected to the reaction cup; the first pump body extracts the diluent into the reaction cup; the first pump body also extracts a certain amount of the first mixed liquid in the reaction cup for temporary storage; after the reaction cup discharges the remaining first mixed liquid, a certain amount of the first mixed liquid is re-introduced into the reaction cup; the first pump body also injects a certain amount of diluent into the reaction cup; The sampling needle extracts a sample and injects it into the reaction cup; the sampling needle extracts a certain amount of reagent and injects it into the reaction cup.

3. The test sample dilution system according to claim 2, characterized in that: It also includes a first tank body, the first tank body stores the diluent, and the input end of the first pump body is connected to the first tank body.

4. The test sample dilution system according to claim 3, characterized in that: It also includes a first three-way solenoid valve, a first port of the first three-way solenoid valve is connected to the first tank body through a pipeline, a second port of the first three-way solenoid valve is connected to the first pump body through a pipeline, and a third port of the first three-way solenoid valve is connected to the reaction cup through a pipeline.

5. The test sample dilution system according to claim 4, characterized in that: It also includes a second three-way solenoid valve arranged on the pipeline between the first three-way solenoid valve and the reaction cup, the first port of the second three-way solenoid valve is connected to the third port of the first three-way solenoid valve through a pipeline, and the second port of the second three-way solenoid valve is connected to the reaction cup through a pipeline.

6. The test sample dilution system according to claim 5, characterized in that: Also includes: a second tank body, wherein the second tank body stores a cleaning liquid; The second pump body, the input end of the second pump body is connected with the second tank body through a pipeline, and the output end of the second pump body is connected with the third port of the second three-way solenoid valve.

7. The test sample dilution system according to claim 6, characterized in that: Also includes: a third pump body, wherein the input end of the third pump body is connected to the reaction cup, and the third pump body pumps out the remaining first mixed liquid in the reaction cup; A waste liquid barrel is communicated with the output end of the third pump body, and the waste liquid barrel stores the first mixed liquid extracted by the third pump body.

8. The test sample dilution system according to claim 7, characterized in that: Also includes: a first solenoid valve, wherein the first solenoid valve is disposed on a pipeline between the first three-way solenoid valve and the second three-way solenoid valve, one port of the first solenoid valve is connected to the third port of the first three-way solenoid valve through a pipeline, and the other port of the first solenoid valve is connected to the first port of the second three-way solenoid valve through a pipeline; The second solenoid valve is placed on the pipeline between the reaction cup and the third pump body, one port of the first solenoid valve is connected to the reaction cup through the pipeline, and the other port of the second solenoid valve is connected to the third pump body through the pipeline.

9. An automatic diluting machine, characterized in that: It comprises a plurality of test sample dilution systems, a host computer and a slave computer as described in any one of claims 2 to 8; A host computer, wherein the host computer is used to input a multiple of dilution required and generate dilution information; A lower computer, wherein the signal input end of the lower computer is connected to the signal output end of the upper computer, and the output end of the lower computer is connected to the detection sample dilution system; the lower computer receives dilution information, and controls the detection sample dilution system to automatically dilute the detection sample according to the dilution information.

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