Dry-type immunochromatography detection instrument and detection method

Abstract

The invention discloses a dry-type immunochromatography detection instrument and a detection method. The dry-type immunochromatography detection instrument comprises a reagent card insertion hole used for inserting a dry-type immunochromatography reagent card containing at least two specific fluorescence labeled antibodies; the light source module is arranged inside the dry-type immunochromatography detection instrument and comprises light sources with at least two wavelengths; the light source module is used for switching a light source required by detection in a time-sharing manner; the optical acquisition plate is arranged inside the dry-type immunochromatography detection instrument and is used for acquiring and amplifying fluorescence signals of various colors generated by the dry-type immunochromatography reagent card; the display screen is arranged on a shell of the dry-type immunochromatography detection instrument and is used for interaction; the lower computer control panel is arranged inside the dry-type immunochromatography detection instrument, is coupled with the optical acquisition board and is coupled with the display screen; and the lower computer control panel is used for generating a detection result according to the acquired data of the optical acquisition board and displaying the detection result on the display screen. The method has relatively high calibration efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of fluorescence detection, and in particular to a dry immunochromatography detection instrument and a detection method. Background Art

[0002] The working principle of dry fluorescent immunochromatographic detection is mainly based on antigen-antibody reaction and fluorescence detection technology. The sample to be tested is placed on a dry reagent strip containing specific fluorescent labeled antibodies, which can specifically bind to the target antigen in the sample to be tested. Subsequently, the reagent strip is illuminated by a light source, causing the fluorescent labeled antibodies to emit fluorescent signals. These signals are captured by the instrument, processed and analyzed, and finally the detection results of the target antigen are obtained.

[0003] However, when performing dry immunochromatographic detection in the prior art, one detection device can only detect one fluorescent signal on the dry reagent strip. Therefore, the dry immunochromatographic detection provided by the prior art has a low detection efficiency. Summary of the invention

[0004] The invention provides a dry immunochromatography detection instrument and a detection method to solve the problem of low detection efficiency of dry immunochromatography.

[0005] According to one aspect of the present invention, there is provided a dry immunochromatographic detection instrument, comprising:

[0006] A reagent card jack, provided on the housing of the dry immunochromatographic detection instrument, for inserting a dry immunochromatographic reagent card containing at least two specific fluorescently labeled antibodies;

[0007] A light source module is arranged inside the dry immunochromatographic detection instrument, and includes light sources of at least two wavelengths; the light source module is used for time-sharing switching of the light sources required for detection;

[0008] An optical collection board is arranged inside the dry immunochromatographic detection instrument, and is used to collect and amplify the various fluorescent signals generated by the dry immunochromatographic reagent card;

[0009] A display screen, arranged on the housing of the dry immunochromatographic detection instrument, for interaction;

[0010] The lower computer control board is arranged inside the dry immunochromatographic detection instrument, coupled to the optical collection board and the display screen; the lower computer control board is used to generate the detection result according to the collected data of the optical collection board and display it on the display screen.

[0011] Optionally, the number of light sources in the light source module corresponds one-to-one to the number of the specific fluorescent-labeled antibodies in the dry immunochromatography reagent card;

[0012] Alternatively, the light source in one of the light source modules corresponds to the specific fluorescent-labeled antibodies in a plurality of the dry immunochromatography reagent cards.

[0013] Optionally, the dry immunochromatography reagent card comprises: a control area and at least two test areas; the number of the test areas corresponds one-to-one to the number of the specific fluorescent-labeled antibodies.

[0014] Optionally, the optical collection board is provided with a fluorescence signal adaptive circuit, and the fluorescence signal adaptive circuit comprises:

[0015] A photosensitive element, used for converting the fluorescent signal into an electrical signal;

[0016] A first-stage amplification module is coupled to the photosensitive element; the first-stage amplification module is used to perform a first amplification on the electrical signal generated by the photosensitive element;

[0017] A digital potentiometer and a second-stage amplification module, wherein the second-stage amplification module is coupled to the output end of the first-stage amplification module, and the second-stage amplification module is coupled to the digital potentiometer; the digital potentiometer is used to adjust the amplification factor of the electrical signal and is output by the second-stage amplification module.

[0018] Optionally, the first-stage amplification module includes a first operational amplifier, a first resistor and a first capacitor; the non-inverting input terminal and the inverting input terminal of the first operational amplifier are respectively coupled to the two ends of the photosensitive element; the output terminal of the first operational amplifier serves as the output terminal of the first-stage amplification module; the two ends of the first resistor are respectively coupled to the inverting input terminal and the output terminal of the first operational amplifier, and the first capacitor is connected in parallel with the first resistor;

[0019] And / or, the second-stage amplification module includes a second operational amplifier, a second resistor and a second capacitor; the non-phase input terminal of the second operational amplifier is coupled to the output terminal of the first-stage amplification module, the inverting input terminal of the second operational amplifier is coupled to the digital potentiometer, and the output terminal of the second operational amplifier serves as the output terminal of the second-stage amplification module; the two ends of the second resistor are respectively coupled to the inverting input terminal and the output terminal of the second operational amplifier, and the second capacitor is connected in parallel with the second resistor.

[0020] Optionally, the dry immunochromatographic detection instrument further comprises:

[0021] A code reader, which is arranged inside the dry immunochromatographic detection instrument and coupled to the lower computer control board; the code reader is used to read the code pattern printed on the dry immunochromatographic reagent card and transmit the reading result to the lower computer control board;

[0022] And / or, further comprising a temperature control module, the temperature control module being arranged inside the dry immunochromatography detection instrument and coupled to the lower computer control board; the temperature control module being used to control the card tray temperature of the dry immunochromatography reagent card to be constant;

[0023] And / or, it also includes a motor, a conveying device and a limit switch, which are arranged inside the dry immunochromatography detection instrument, the conveying device is used to convey the dry immunochromatography reagent card to a set position, and the limit switch is arranged at the set position of the conveying device; the motor and the limit switch are coupled to the lower computer control board and controlled by the lower computer control board.

[0024] According to another aspect of the present invention, a dry immunochromatographic detection method is provided, characterized in that a dry immunochromatographic detection instrument as described in any embodiment of the present invention is used; the method comprises:

[0025] Exciting and collecting at least two fluorescent signals of the dry immunochromatographic test paper card in a time-sharing manner;

[0026] Detecting the signal intensity of each of the fluorescent signals, and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity;

[0027] Curve fitting and concentration calculation of corresponding items are performed according to each of the signal intensities and the corresponding amplification factors.

[0028] Optionally, the time-sharing excitation and collection of at least two fluorescent signals of the dry immunochromatographic test paper card includes:

[0029] Using a pulse width modulation method to drive the light sources of at least two wavelengths, and controlling the light sources to light up in time;

[0030] After one of the light sources is lit, the optical collection board collects the fluorescence signal on the dry immunochromatographic test paper card corresponding to the lit light source.

[0031] Optionally, before detecting the signal intensity of each of the fluorescent signals and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity, the method further includes:

[0032] storing the raw data of at least two fluorescent signals in designated data channels respectively according to corresponding excitation wavelengths;

[0033] And, detecting the signal intensity of each of the fluorescent signals and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity includes:

[0034] The signal strength of the original data of each data channel is detected in real time. If the signal strength exceeds a preset upper limit, the amplification factor of the corresponding data channel signal is dynamically increased by a multiple of 2; if the signal strength is lower than a lower limit, the amplification factor of the corresponding data channel signal is dynamically reduced by a multiple of 2.

[0035] Optionally, performing curve fitting and concentration calculation of corresponding items according to each of the signal intensities and the corresponding amplification factors includes:

[0036] According to the data of each data channel and the magnification, the data of each data channel is respectively fitted with a polynomial to obtain a data curve; wherein the abscissa of the data curve is the position of the data of each data channel on the dry immunochromatography reagent card, and the ordinate is the data of the data channel;

[0037] Calculate the data peak value and area of ​​each data channel according to the data curve;

[0038] The concentration of the corresponding item is calculated according to the data peak value and area of ​​each data channel.

[0039] The technical solution provided by the embodiment of the present invention, by setting a light source module with a plurality of light sources of different wavelengths, can time-share the input and switching of the light source under the control of the optical collection board, so that the dry immunochromatography reagent card containing a plurality of specific fluorescent-labeled antibodies generates different fluorescent signals according to the light sources of different wavelengths. And through the collection and amplification of each fluorescent signal by the optical collection board, the lower computer control board can generate the test result according to the collected data of the optical collection board, thereby realizing the concentration detection of each target antigen. The present invention can realize the detection of multiple target antigens through the time-shared switching of the light source and irradiation of the dry immunochromatography reagent card containing a plurality of specific fluorescent-labeled antibodies through one dry immunochromatography reagent card, and has a high calibration efficiency. And there is no need to use multiple detection equipment and multiple dry immunochromatography reagent cards, and has a low detection cost.

[0040] It should be understood that the contents described in this section are not intended to identify the key or important features of the embodiments of the present invention, nor are they intended to limit the scope of the present invention. Other features of the present invention will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0042] Figure 1 is an appearance diagram of a dry immunochromatographic detection instrument provided according to an embodiment of the present invention;

[0043] Figure 2 is a module diagram of a dry immunochromatographic detection instrument provided according to an embodiment of the present invention;

[0044] Figure 3 is an appearance diagram of a dry immunochromatography reagent card provided according to an embodiment of the present invention;

[0045] Figure 4 is a circuit diagram of a fluorescence signal adaptive circuit provided according to an embodiment of the present invention

[0046] Figure 5 is a top view of the internal structure of a dry immunochromatographic detection instrument provided according to an embodiment of the present invention;

[0047] Figure 6 is a side view of the internal structure of a dry immunochromatographic detection instrument provided according to an embodiment of the present invention;

[0048] Figure 7 is a module diagram of another dry immunochromatographic detection instrument provided according to an embodiment of the present invention;

[0049] Figure 8 is a flow chart of a dry immunochromatographic detection method provided according to an embodiment of the present invention;

[0050] Fig. 9 is a flow chart of another dry immunochromatographic detection method provided according to an embodiment of the present invention;

[0051] Fig.10 It is a flow chart of another dry immunochromatography detection method provided according to an embodiment of the present invention. DETAILED DESCRIPTION

[0052] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0053] It should be noted that the terms "first", "second", etc. in the specification and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the present invention described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units that are clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0054] The embodiment of the present invention provides a dry immunochromatographic detection instrument. Figure 1 The figure is an appearance diagram of a dry immunochromatographic detection instrument provided by an embodiment of the present invention. Figure 2 A module diagram of a dry immunochromatographic detection instrument provided by an embodiment of the present invention. Figure 1 and Figure 2 The dry immunochromatographic detection instrument comprises: a reagent card jack 5, a light source module 1, an optical collection board 2, a display screen 4 and a lower computer control board 3. The reagent card jack 5 is arranged on the outer shell of the dry immunochromatographic detection instrument, and is used to insert a dry immunochromatographic reagent card 6 containing at least two specific fluorescent labeled antibodies. The light source module 1 is arranged inside the dry immunochromatographic detection instrument, and includes a light source of at least two wavelengths; the light source module 1 is used to time-sharingly switch the light source required for detection. The optical collection board 2 is arranged inside the dry immunochromatographic detection instrument, and the optical collection board 2 is used to collect and amplify the various color fluorescent signals generated by the dry immunochromatographic reagent card 6. The display screen 4 is arranged on the outer shell of the dry immunochromatographic detection instrument for interaction. The lower computer control board 3 is arranged inside the dry immunochromatographic detection instrument, coupled with the optical collection board 2, and coupled with the display screen 4; the lower computer control board 3 is used to generate the detection result according to the collected data of the optical collection board 2, and display it on the display screen 4.

[0055] The dry immunochromatographic reagent card 6 has at least two specific fluorescently labeled antibodies, and when the sample to be tested is placed on the dry immunochromatographic reagent card 6, the specific fluorescently labeled antibodies can specifically bind to the target antigen in the sample. For example, the sample to be tested can be a blood sample, and the target antigen can include viruses, bacteria, hormones, tumor markers, etc. Figure 1 The dry immunochromatographic detection instrument is exemplarily shown to have three reagent card insertion holes 5, and three dry immunochromatographic reagent cards 6 can be detected at the same time.

[0056] The dry immunochromatography reagent card 6 can be used to bind a specific fluorescently labeled antibody to a target antigen. The light source module 1 excites the light source to generate monochromatic light close to the absorption wavelength of the specific fluorescently labeled antibody and irradiates the dry immunochromatography reagent card 6, so that the specific fluorescently labeled antibody after binding to the target antigen can emit a fluorescent signal. These fluorescent signals can be collected and amplified by the optical collection board 2. For example, the optical collection board 2 can convert the fluorescent signal into an electrical signal, and the lower computer control board 3 calculates and analyzes the electrical signal converted by the optical collection board 2 to obtain the concentration of the detected target antigen.

[0057] The dry immunochromatography reagent card 6 provided by the present invention has a plurality of specific fluorescently labeled antibodies, and the light source module 1 can generate light sources of different wavelengths in a time-sharing manner. Therefore, the detection of a plurality of target antigens can be achieved through a dry immunochromatography detection instrument and a dry immunochromatography reagent card 6. Exemplarily, the dry immunochromatography reagent card 6 has 5 specific fluorescently labeled antibodies. When each specific fluorescently labeled antibody can bind to a target antigen, the dry immunochromatography reagent card 6 can be irradiated by switching five light sources of different wavelengths in a time-sharing manner, so that the 5 specific fluorescently labeled antibodies generate 5 different fluorescent signals correspondingly.

[0058] Specifically, the light source module 1 emits a light source of a wavelength corresponding to a specific fluorescent-labeled antibody for irradiation, and the specific fluorescent-labeled antibody generates a corresponding fluorescent signal and is collected by the optical collection board 2. After a short delay, the light source module 1 switches to another light source of a wavelength corresponding to a specific fluorescent-labeled antibody for irradiation, and the specific fluorescent-labeled antibody generates a corresponding fluorescent signal and is collected by the optical collection board 2. The light source module 1 generates five light sources of different wavelengths by time-sharing, so that the dry immunochromatography reagent card 6 generates five different fluorescent signals. The duration of the light source module 1 emitting a light source is extremely short, generally in the millisecond level, so even if the light source module 1 irradiates five times in time-sharing, it can be completed in a very short time.

[0059] After each irradiation of the light source module 1, the optical collection board 2 receives the fluorescent signal generated by this irradiation and amplifies the signal. The amplified signal can be an electrical signal. The lower computer control board 3 analyzes the electrical signal to generate the concentration of the target antigen and displays it on the display screen 4. Exemplarily, the display screen 4 can be a touch screen, and the dry immunochromatography detection instrument can be debugged and test instructions can be issued through the touch screen.

[0060] When the light source module 1 completes the time-sharing irradiation and the optical collection board 2 collects all the fluorescent signals, the dry immunochromatography detection instrument can push out the dry immunochromatography reagent card 6 to complete the detection of the dry immunochromatography reagent card 6. At this time, the test results can be viewed through the display screen 4. The dry immunochromatography detection instrument can also be provided with a printer 12, which can be a thermal printer, and the test results can be printed through the printer 12.

[0061] The technical solution provided by the embodiment of the present invention, by setting a light source module 1 with a plurality of light sources of different wavelengths, can be used for time-sharing input and switching of the light source under the control of the optical collection board 2, so that the dry immunochromatography reagent card 6 containing a plurality of specific fluorescent-labeled antibodies generates different fluorescent signals according to the light sources of different wavelengths. And through the collection and amplification of each fluorescent signal by the optical collection board 2, the lower computer control board 3 can generate the detection result according to the collected data of the optical collection board 2, thereby realizing the concentration detection of each target antigen. The present invention can realize the detection of multiple target antigens through time-sharing switching of the light source and irradiation of the dry immunochromatography reagent card 6 containing a plurality of specific fluorescent-labeled antibodies through one dry immunochromatography reagent card 6, and has a high calibration efficiency. And there is no need to use multiple detection equipment and multiple dry immunochromatography reagent cards 6, which has a low detection cost.

[0062] Based on the above embodiments, optionally, the number of light sources in the light source module corresponds one-to-one to the number of specific fluorescent-labeled antibodies in the dry immunochromatography reagent card; or, the light sources in one light source module correspond to the specific fluorescent-labeled antibodies in multiple dry immunochromatography reagent cards.

[0063] Among them, a specific fluorescently labeled antibody can correspond to one target antigen or multiple target antigens, which can be determined according to the characteristics of the specific fluorescently labeled antibody and the target antigen. For example, when two of the specific fluorescently labeled antibodies in the dry immunochromatography reagent card have the same characteristics, the light source that can be absorbed by these two specific fluorescently labeled antibodies is monochromatic light with the same wavelength. When performing time-sharing irradiation of the light source module 1 and switching the light source corresponding to the two specific fluorescently labeled antibodies, it is necessary to switch to monochromatic light with the same wavelength.

[0064] Figure 3The figure is an appearance diagram of a dry immunochromatography reagent card provided by an embodiment of the present invention. Based on the above embodiments, optionally, the dry immunochromatography reagent card 6 includes: a control area C1 and at least two test areas T; the number of test areas T corresponds to the number of specific fluorescent labeled antibodies.

[0065] After the sample to be tested is placed in the sampling area A1 of the dry immunochromatographic reagent card 6, the sample to be tested will slowly penetrate into the control area C1 and the test area T. When the control area C1 is colored, it means that the test of the test area T of the dry immunochromatographic reagent card 6 is valid. Figure 3 A dry immunochromatographic reagent card having three test areas T is exemplarily shown, where the test area T1 , the test area T2 and the test area T3 can be used to test different target antigens.

[0066] When the dry immunochromatographic reagent card 6 is tested, the light source module 1 irradiates in time according to the wavelength of the light source required for the test area T1, the test area T2 and the test area T3. Exemplarily, the light source module 1 emits a light source with a wavelength corresponding to the test area T1 for irradiation, and stops irradiating after a set time. Afterwards, the light source module 1 emits a light source with a wavelength corresponding to the test area T2 for irradiation, and stops irradiating after a set time. Afterwards, the light source module 1 emits a light source with a wavelength corresponding to the test area T3 for irradiation. If the target antigens tested in each test area T are combined with a specific fluorescent-labeled antibody with the same characteristics. The light source of the same wavelength can also be used to irradiate each test area T in time.

[0067] Figure 4 A circuit diagram of a fluorescence signal adaptive circuit provided in an embodiment of the present invention. Based on the above embodiments, optionally, the optical acquisition board is provided with a fluorescence signal adaptive circuit, and the fluorescence signal adaptive circuit includes: a photosensitive element D1, a first-stage amplification module 21, a digital potentiometer 23 and a second-stage amplification module 22. The photosensitive element D1 is used to convert the fluorescence signal into an electrical signal. The first-stage amplification module 21 is coupled to the photosensitive element D1; the first-stage amplification module 21 is used to perform a first amplification on the electrical signal generated by the photosensitive element D1. The second-stage amplification module 22 is coupled to the output end of the first-stage amplification module 21, and the second-stage amplification module 22 is coupled to the digital potentiometer 23; the digital potentiometer 23 is used to adjust the amplification factor of the electrical signal, and is output by the second-stage amplification module 22.

[0068] Among them, the value of the electrical signal directly converted by the photosensitive element D1 is small, which is not convenient for subsequent processing and calculation. The value of the electrical signal converted by the photosensitive element D1 can be amplified once by the first-stage amplification module 21. When the electrical signal is too large or too small, the optical acquisition board may not be able to sample the voltage signal well, which will cause errors in the detection results. Therefore, the amplification factor can be adjusted by the digital potentiometer. The electrical signal output by the first-stage amplification module 21 is input into the second-stage amplification module 22. The digital potentiometer 23 can adjust the amplification factor of the second-stage amplification module 22 and make the electrical signal meet the acquisition requirements of the built-in circuit of the optical acquisition board. Exemplarily, the electrical signal includes a voltage signal. When the electrical signal is close to the upper limit of the built-in circuit of the optical acquisition board, the amplification factor of the second-stage amplification module 22 can be increased. On the contrary, if the electrical signal is close to the lower limit of the built-in circuit of the optical acquisition board, the amplification factor of the second-stage amplification module 22 can be reduced.

[0069] Continue to refer Figure 4 , based on the above embodiments, optionally, the first-stage amplification module 21 includes a first operational amplifier U1, a first resistor R1 and a first capacitor C1; the positive input terminal and the negative input terminal of the first operational amplifier U1 are respectively coupled to the two ends of the photosensitive element D1; the output terminal of the first operational amplifier U1 serves as the output terminal of the first-stage amplification module 21; the two ends of the first resistor R1 are respectively coupled to the negative input terminal and the output terminal of the first operational amplifier U1, and the first capacitor C1 is connected in parallel with the first resistor R1. And / or, the second-stage amplification module 22 includes a second operational amplifier U2, a second resistor R2 and a second capacitor C2; the positive input terminal of the second operational amplifier U2 is coupled to the output terminal of the first-stage amplification module 21, the negative input terminal of the second operational amplifier U2 is coupled to the digital potentiometer 23, and the output terminal of the second operational amplifier U2 serves as the output terminal of the second-stage amplification module 22; the two ends of the second resistor R2 are respectively coupled to the negative input terminal and the output terminal of the second operational amplifier U2, and the second capacitor C2 is connected in parallel with the second resistor R2.

[0070] Among them, the first resistor R1 and the first capacitor C1 are connected in series between the inverting input terminal and the output terminal of the first operational amplifier U1. The first resistor R1 can be used to stabilize the gain of the first operational amplifier U1, and the first capacitor C1 can be used to compensate for the phase shift that may be generated by the first operational amplifier U1, so that the amplifier works more stably. The electrical signal amplified by the first operational amplifier U1 is input into the non-inverting input terminal of the second operational amplifier U2, and the digital potentiometer 23 is coupled to the inverting input terminal of the second operational amplifier U2. By adjusting the adjustable resistor in the digital potentiometer 23, the amplification factor of the second operational amplifier U2 can be adjusted, thereby continuing to amplify or reduce the electrical signal.

[0071] Figure 5 A top view of the internal structure of a dry immunochromatographic detection instrument provided by an embodiment of the present invention. Figure 5On the basis of the above embodiments, optionally, the dry immunochromatographic detection instrument further comprises: a code reader 7, which is arranged inside the dry immunochromatographic detection instrument and coupled to the lower computer control board; the code reader 7 is used to read the code pattern printed on the dry immunochromatographic reagent card 6 and transmit the reading result to the lower computer control board.

[0072] Among them, a code pattern can be set on the surface of the dry immunochromatography reagent card 6 to store the test information to be detected by the reagent card. Before testing the dry immunochromatography reagent card 6, the code pattern is first read by the code reader 7. Exemplarily, the code pattern can be a two-dimensional code pattern. The lower computer control board inputs the test information of the immunochromatography reagent card 6 into the optical acquisition board, and the optical acquisition board implements the setting of the scanning parameters according to the test information of the immunochromatography reagent card 6, and controls the light source module to generate light sources of different wavelengths in a time-sharing manner.

[0073] Continue to refer Figure 5 On the basis of the above embodiments, optionally, the dry immunochromatography detection instrument further includes a temperature control module, which is arranged inside the dry immunochromatography detection instrument and coupled to the lower computer control board; the temperature control module is used to control the temperature of the tray 8 of the dry immunochromatography reagent card 6 to be constant.

[0074] In order to ensure the activity and stability of the sample to be tested placed in the dry immunochromatography reagent card 6, the dry immunochromatography reagent card 6 can be placed in a dry immunochromatography detection instrument for incubation. The temperature of the card tray 8 can be controlled to be constant by setting a temperature control module, thereby keeping the temperature of the dry immunochromatography reagent card 6 constant. For example, the temperature can be 37 degrees Celsius.

[0075] Figure 6 A side view of the internal structure of a dry immunochromatographic detection instrument provided by an embodiment of the present invention. Figure 5 and Figure 6 On the basis of the above embodiments, optionally, the dry immunochromatographic detection instrument further includes a motor 9, a conveying device 10 and a limit switch 11, which are arranged inside the dry immunochromatographic detection instrument, the conveying device 10 is used to convey the dry immunochromatographic reagent card 6 to a set position, and the limit switch 11 is arranged at the set position of the conveying device 10; the motor 9 and the limit switch 11 are both coupled to the lower computer control board and controlled by the lower computer control board.

[0076] The conveying device 10 includes an idler wheel 101, a conveyor belt 102, a guide rail 103 and a transmission wheel 104. The transmission wheel 104 and the idler wheel 101 are used to support the conveyor belt 102, and the transmission wheel 104 is driven to rotate by the motor 9, thereby driving the conveyor belt 102 to run. The conveyor belt 102 is connected to the card tray 8, and can drive the card tray 8 to move on the guide rail 103.

[0077] When the dry immunochromatographic reagent card 6 is inserted into the dry immunochromatographic detection instrument through the reagent card insertion hole 5, the limit switch 11 can be used to limit the insertion position of the dry immunochromatographic reagent card 6. When the dry immunochromatographic detection instrument is turned on and the insertion position of the dry immunochromatographic reagent card 6 is correct, the motor 9 is operated, and the conveying device 10 drives the dry immunochromatographic reagent card 6 to move to the code reader 7 for reading the code pattern. The code reader 7 transmits the reading result to the lower computer control board, and the optical acquisition board generates test items of the test area T1, the test area T2 and the test area T3 in the dry immunochromatographic reagent card 6 according to the reading result, and generates different control signals according to the test items to control the light source module 1 to generate light sources of different wavelengths.

[0078] Exemplarily, the conveying device 10 aligns the test area T1 of the dry immunochromatography reagent card 6 with the light output end of the light source module 1, and the light source module 1 generates a light source of a certain wavelength according to the test items of the test area T1 and irradiates the test area T1. After the setting time, the light source module 1 is turned off, the conveying device 10 moves the dry immunochromatography reagent card 6 and aligns the test area T2 with the light output end of the light source module 1, and the light source module 1 generates a light source of a certain wavelength according to the test items of the test area T2 and irradiates the test area T2. After the setting time, the light source module 1 is turned off, the conveying device 10 moves the dry immunochromatography reagent card 6 and aligns the test area T3 with the light output end of the light source module 1, and the light source module 1 generates a light source of a certain wavelength according to the test items of the test area T3 and irradiates the test area T3. After the setting time, the light source module 1 is turned off, and the conveying device 10 can partially eject the dry immunochromatography reagent card 6 from the dry immunochromatography detection instrument, which is convenient for relevant personnel to recycle the dry immunochromatography reagent card 6.

[0079] Figure 7 This is a module diagram of another dry immunochromatographic detection instrument provided by an embodiment of the present invention. Figure 7 Optionally, the dry immunochromatographic detection instrument further includes a main control board 13, which is connected between the display screen 4 and the lower computer control board 3, and can be used for interaction and data transmission between the display screen 4 and the lower computer control board 3, and store the detection results of the dry immunochromatographic detection instrument.

[0080] The embodiment of the present invention further provides a dry immunochromatographic detection method, which uses the dry immunochromatographic detection instrument provided by any embodiment of the present invention. Figure 8 A flow chart of a dry immunochromatographic detection method provided by an embodiment of the present invention. Figure 8 , the method comprising:

[0081] S110, exciting and collecting at least two fluorescent signals of the dry immunochromatographic test paper card in a time-sharing manner.

[0082] Among them, the dry immunochromatographic test paper card can have multiple test areas, and the light source module can generate light and illuminate each test area one by one in a time-sharing manner. The wavelength of the light source irradiated into each test area can be the same wavelength or different wavelengths, which can be specifically determined by the test items of the test area. Each test area in the dry immunochromatographic test paper card generates a fluorescent signal according to the light source irradiated into it.

[0083] S120, detecting the signal intensity of each fluorescent signal, and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity.

[0084] Among them, electrical signals of different sizes can be generated according to the signal strength of each fluorescent signal, and the electrical signal can be a voltage signal. When the voltage signal is too large or too small, the optical acquisition board may not be able to sample the voltage signal well, resulting in errors in the detection results. Therefore, it is necessary to dynamically adjust the amplification factor of the fluorescent signal. Exemplarily, when the signal intensity is close to the upper limit of the built-in circuit of the optical acquisition board, the amplification factor of the fluorescent signal is dynamically set by the fluorescent signal adaptive circuit, and the amplification factor of the fluorescent signal is increased. Conversely, if the signal intensity of the data of each channel is low, the amplification factor of the fluorescent signal is dynamically set by the fluorescent signal adaptive circuit, and the amplification factor of the fluorescent signal is reduced.

[0085] S130, performing curve fitting and concentration calculation of corresponding items according to each signal intensity and each corresponding amplification factor.

[0086] The concentration of the corresponding item can be calculated by each signal intensity and each corresponding magnification. Specifically, a curve is fitted by each signal intensity and each corresponding magnification, and the abscissa of the curve can be used to represent the position of the tested test area, and the ordinate of the curve can be used to represent the signal intensity at the position. The concentration of the corresponding item can be calculated by the maximum signal intensity of the fluorescent signal in the test area and the area of ​​the luminescent area with the fluorescent signal.

[0087] The technical solution provided by the embodiment of the present invention can generate light sources of different wavelengths by time-sharing excitation, so that the dry immunochromatographic test paper card can generate different fluorescent signals according to the light sources of different wavelengths, thereby realizing the detection of multiple detection items in the dry immunochromatographic test paper card. The present invention can detect multiple target antigens through a dry immunochromatographic reagent card by time-sharing switching of the light source and irradiating the dry immunochromatographic reagent card containing multiple specific fluorescent labeled antibodies, and has a high calibration efficiency. And there is no need to use multiple detection equipment and multiple dry immunochromatographic reagent cards, which has a lower detection cost.

[0088] Fig. 9 A flow chart of another dry immunochromatographic detection method provided by an embodiment of the present invention. Fig. 9On the basis of the above embodiments, optionally, S110, time-sharing excitation and collection of at least two fluorescent signals of the dry immunochromatographic test paper card includes:

[0089] S111, using a pulse width modulation method to drive light sources of at least two wavelengths, and controlling the light sources to light up in a time-sharing manner.

[0090] The optical acquisition board can control the light source module according to the pulse width modulation method (PWM), and the frequency range of the pulse width modulation can be 20Hz-2KHz. For example, the light source module can include 8 different wavelengths of monochromatic light, and the optical acquisition board can achieve time-sharing drive and control of the 8 different wavelengths of monochromatic light by adjusting the duty cycle of the pulse width.

[0091] S112. After a light source is turned on, the optical collection board collects the fluorescence signal of the corresponding light source on the dry immunochromatographic test paper card.

[0092] When a light source is turned on, the collection area illuminated by the collection dry immunochromatographic test paper card will generate a fluorescent signal according to the light source. The optical collection board can collect the fluorescent signal.

[0093] Based on the above embodiments, optionally, before detecting the signal intensity of each fluorescent signal and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity, it also includes: storing the original data of at least two fluorescent signals in designated data channels according to the corresponding excitation wavelengths.

[0094] When there are a lot of fluorescence signal data, in order to prevent data confusion, the original data of each fluorescence signal can be stored independently after being matched with the excitation wavelength, and the original data of each fluorescence signal can correspond to a data channel.

[0095] On the basis of the above embodiments, optionally, the signal strength of each fluorescent signal is detected, and the amplification factor of the fluorescent signal is dynamically adjusted according to the signal strength, including: real-time detection of the signal strength of the original data of each data channel, if the signal strength exceeds a preset upper limit, dynamically increasing the amplification factor of the corresponding data channel signal by a multiple of 2; if the signal strength is lower than a lower limit, dynamically reducing the amplification factor of the corresponding data channel signal by a multiple of 2.

[0096] Exemplarily, the embodiment of the present invention can adjust the data channel signal amplification factor through a digital potentiometer. Each time the digital potentiometer is adjusted, the corresponding amplification factor can be increased or decreased by one time. When the signal strength exceeds the preset upper limit, it means that the signal exceeds the acquisition requirement of the built-in circuit of the optical acquisition board. At this time, the optical acquisition board cannot collect the signal well. Therefore, the amplification factor of the corresponding data channel signal can be dynamically increased by a multiple of 2 through the digital potentiometer, and the signal meets the acquisition requirement of the built-in circuit of the optical acquisition board. When the signal strength is lower than the preset lower limit, it means that the built-in circuit of the optical acquisition board cannot effectively collect the signal. Therefore, the amplification factor of the corresponding data channel signal can be dynamically reduced by a multiple of 2 through the digital potentiometer, and the signal meets the acquisition requirement of the built-in circuit of the optical acquisition board.

[0097] Fig.10 A flow chart of another dry immunochromatographic detection method provided by an embodiment of the present invention. Fig.10 Based on the above embodiments, optionally, S130, performing curve fitting and concentration calculation of corresponding items according to each signal intensity and each corresponding magnification factor, includes:

[0098] S131, according to the data of each data channel and the magnification, respectively perform polynomial fitting on the data of each data channel to obtain a data curve, wherein the abscissa of the data curve is the position of the data of each data channel on the dry immunochromatography reagent card, and the ordinate is the data of the data channel.

[0099] S132. Calculate the data peak value and area of ​​each data channel according to the data curve.

[0100] S133, calculating the concentration of the corresponding item according to the data peak value and area of ​​each data channel.

[0101] Among them, when performing the detection of the dry immunochromatographic reagent card, each test area needs to be illuminated in a time-sharing manner. Therefore, each time the illumination is performed, a test area will generate a set of fluorescent signals. After processing the signal and performing polynomial fitting, a data curve can be obtained. The data peak can be used to reflect the maximum intensity of the fluorescent signal, and the area can be used to reflect the area of ​​the fluorescent signal emitted under the illumination. According to the data peak and area of ​​this data channel, the concentration of the item detected in the test area can be calculated. By calculating the concentration of the items detected in each test area separately, the concentration of the items detected in all test areas can be obtained, and the detection of all test areas in the dry immunochromatographic reagent card can be completed.

[0102] It should be understood that the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps described in the present invention can be executed in parallel, sequentially or in different orders, as long as the desired results of the technical solution of the present invention can be achieved, and this document does not limit this.

[0103] The above specific implementations do not constitute a limitation on the protection scope of the present invention. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions can be made according to design requirements and other factors. Any modification, equivalent substitution and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A dry immunochromatographic detection instrument, characterized in that: include: A reagent card jack, provided on the housing of the dry immunochromatographic detection instrument, for inserting a dry immunochromatographic reagent card containing at least two specific fluorescently labeled antibodies; A light source module is arranged inside the dry immunochromatographic detection instrument, and includes light sources of at least two wavelengths; the light source module is used for time-sharing switching of the light sources required for detection; An optical collection board is arranged inside the dry immunochromatographic detection instrument, and is used to collect and amplify the various fluorescent signals generated by the dry immunochromatographic reagent card; A display screen, arranged on the housing of the dry immunochromatographic detection instrument, for interaction; The lower computer control board is arranged inside the dry immunochromatographic detection instrument, coupled to the optical collection board and the display screen; the lower computer control board is used to generate the detection result according to the collected data of the optical collection board and display it on the display screen.

2. The dry immunochromatographic detection instrument according to claim 1, characterized in that: The number of light sources in the light source module corresponds one-to-one to the number of the specific fluorescent-labeled antibodies in the dry immunochromatography reagent card; Alternatively, the light source in one of the light source modules corresponds to the specific fluorescent-labeled antibodies in a plurality of the dry immunochromatography reagent cards.

3. The dry immunochromatographic detection instrument according to claim 2, characterized in that: The dry immunochromatographic reagent card comprises: a control area and at least two test areas; the number of the test areas corresponds one-to-one to the number of the specific fluorescent-labeled antibodies.

4. The dry immunochromatographic detection instrument according to claim 1, characterized in that: The optical collection board is provided with a fluorescence signal adaptive circuit, and the fluorescence signal adaptive circuit comprises: A photosensitive element, used for converting the fluorescent signal into an electrical signal; A first-stage amplification module is coupled to the photosensitive element; the first-stage amplification module is used to perform a first amplification on the electrical signal generated by the photosensitive element; A digital potentiometer and a second-stage amplification module, wherein the second-stage amplification module is coupled to the output end of the first-stage amplification module, and the second-stage amplification module is coupled to the digital potentiometer; the digital potentiometer is used to adjust the amplification factor of the electrical signal and is output by the second-stage amplification module.

5. The dry immunochromatographic detection instrument according to claim 4, characterized in that: The first-stage amplification module includes a first operational amplifier, a first resistor and a first capacitor; the non-inverting input terminal and the inverting input terminal of the first operational amplifier are respectively coupled to the two ends of the photosensitive element; the output terminal of the first operational amplifier serves as the output terminal of the first-stage amplification module; the two ends of the first resistor are respectively coupled to the inverting input terminal and the output terminal of the first operational amplifier, and the first capacitor is connected in parallel with the first resistor; And / or, the second-stage amplification module includes a second operational amplifier, a second resistor and a second capacitor; the non-phase input terminal of the second operational amplifier is coupled to the output terminal of the first-stage amplification module, the inverting input terminal of the second operational amplifier is coupled to the digital potentiometer, and the output terminal of the second operational amplifier serves as the output terminal of the second-stage amplification module; the two ends of the second resistor are respectively coupled to the inverting input terminal and the output terminal of the second operational amplifier, and the second capacitor is connected in parallel with the second resistor.

6. The dry immunochromatographic detection instrument according to claim 1, characterized in that: Also includes: A code reader, which is arranged inside the dry immunochromatographic detection instrument and coupled to the lower computer control board; the code reader is used to read the code pattern printed on the dry immunochromatographic reagent card and transmit the reading result to the lower computer control board; And / or, further comprising a temperature control module, the temperature control module being arranged inside the dry immunochromatography detection instrument and coupled to the lower computer control board; the temperature control module being used to control the card tray temperature of the dry immunochromatography reagent card to be constant; And / or, it also includes a motor, a conveying device and a limit switch, which are arranged inside the dry immunochromatography detection instrument, the conveying device is used to convey the dry immunochromatography reagent card to a set position, and the limit switch is arranged at the set position of the conveying device; the motor and the limit switch are coupled to the lower computer control board and controlled by the lower computer control board.

7. A dry immunochromatographic detection method, characterized in that: Using the dry immunochromatographic detection instrument according to any one of claims 1 to 6; the method comprises: Exciting and collecting at least two fluorescent signals of the dry immunochromatographic test paper card in a time-sharing manner; Detecting the signal intensity of each of the fluorescent signals, and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity; Curve fitting and concentration calculation of corresponding items are performed according to each of the signal intensities and the corresponding amplification factors.

8. The dry immunochromatographic detection method according to claim 7, characterized in that: The time-sharing excitation and collection of at least two fluorescent signals of the dry immunochromatographic test paper card includes: Using a pulse width modulation method to drive the light sources of at least two wavelengths, and controlling the light sources to light up in time; After one of the light sources is lit, the optical collection board collects the fluorescence signal on the dry immunochromatographic test paper card corresponding to the lit light source.

9. The dry immunochromatographic detection method according to claim 7, characterized in that: Before detecting the signal intensity of each of the fluorescent signals and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity, the method further includes: storing the raw data of at least two fluorescent signals in designated data channels respectively according to corresponding excitation wavelengths; And, detecting the signal intensity of each of the fluorescent signals and dynamically adjusting the amplification factor of the fluorescent signal according to the signal intensity includes: The signal strength of the original data of each data channel is detected in real time. If the signal strength exceeds a preset upper limit, the amplification factor of the corresponding data channel signal is dynamically increased by a multiple of 2; if the signal strength is lower than a lower limit, the amplification factor of the corresponding data channel signal is dynamically reduced by a multiple of 2.

10. The dry immunochromatographic detection method according to claim 9, characterized in that: The curve fitting and concentration calculation of the corresponding items according to the signal intensities and the corresponding magnifications include: According to the data of each data channel and the magnification, the data of each data channel is respectively fitted with a polynomial to obtain a data curve; wherein the abscissa of the data curve is the position of the data of each data channel on the dry immunochromatography reagent card, and the ordinate is the data of the data channel; Calculate the data peak value and area of ​​each data channel according to the data curve; The concentration of the corresponding item is calculated according to the data peak value and area of ​​each data channel.

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