Cosmetic composition capable of resisting oxidation free radicals, application of cosmetic composition and cosmetic

Abstract

According to the antioxidant free radical cosmetic composition and the application thereof and the cosmetic, the antioxidant free radical cosmetic composition is prepared from vitamin C, glutathione and tocopheryl nicotinate according to the mass ratio of (20-35): (3-5): 1, the composition of vitamin C, glutathione and tocopheryl nicotinate is provided, and the proportion is screened and optimized, so that the antioxidant free radical cosmetic composition is obtained. The synergistic effect of the three components in the aspect of improving the antioxidant capacity of the skin is researched, and the synergistic effect can be realized through various mechanisms, including improving the concentration of antioxidants in the skin, prolonging the activity time of the antioxidants and playing a complementary role in different skin levels, so that the optimal synergistic antioxidant effect of the components is realized; the skin care effect is fully exerted, a scientific basis is provided for the application of the composition in a skin care product formula, and the composition has very strong practicability and wide applicability.

Description

Technical Field

[0001] The invention relates to a cosmetic composition, in particular to an antioxidant free radical cosmetic composition and application thereof, and cosmetics, belonging to the technical field of cosmetics. Background Art

[0002] As the largest organ in the human body, the skin not only protects the body from external environmental damage, but also is an important manifestation of beauty. With people's constant pursuit of health and beauty, the skin care market has ushered in rapid development. However, the challenges facing skin health are also increasing, one of which is oxidative stress. Ultraviolet radiation, environmental pollution, life stress and other factors can lead to excessive production of free radicals in the skin, which in turn cause skin aging, pigmentation, skin cancer and other problems.

[0003] In order to resist oxidative stress, various antioxidants are widely used in the field of skin care. Common ingredients include: vitamin C and its derivatives, vitamin E and its derivatives, glutathione, niacin and niacinamide. These four ingredients have different antioxidant mechanisms: Vitamin C is a water-soluble antioxidant that mainly captures water-soluble free radicals, reduces oxidative stress, promotes collagen synthesis, and inhibits melanin deposition; Vitamin E is a fat-soluble antioxidant that protects the lipids of cell membranes and organelles from oxidative damage mainly by blocking free radical chain reactions. It can also regenerate oxidized vitamin C and enhance its antioxidant effect. Glutathione helps maintain redox balance in cells, protects cells from oxidative damage, and can regenerate oxidized vitamin C and vitamin E; Niacin and niacinamide mainly promote cellular energy metabolism, reduce oxidative stress, regulate the skin barrier, enhance water retention capacity, reduce fine lines and spots, and improve uneven skin tone.

[0004] It can be seen that these ingredients have different focuses in their antioxidant free radical effects. Vitamin C mainly acts on the aqueous phase area within the cell, vitamin E mainly acts on the cell membrane and lipid area, glutathione plays an important role in redox within the cell, while niacin and niacinamide can promote cellular energy metabolism.

[0005] Existing skin care products on the market often use these four components, either one or more, but they all use the unique efficacy of each component. There is a lack of corresponding research on the use of multiple components, especially the synergistic effect. Summary of the invention

[0006] To address the deficiencies of the prior art, the present invention aims to provide a cosmetic composition for resisting oxidative free radicals and its application and cosmetics.

[0007] In order to achieve the above object, the present invention adopts the following technical solution: A cosmetic composition for resisting oxidative free radicals comprises vitamin C, glutathione and tocopherol nicotinate in a mass ratio of (20-35):(3-5):1.

[0008] The mass ratio of the above vitamin C, glutathione and tocopherol nicotinate is 35:5:1.

[0009] The above-mentioned cosmetic composition is used for preparing cosmetics.

[0010] Furthermore, the mass content of the above-mentioned cosmetic composition in cosmetics is 0.01-0.03%.

[0011] Furthermore, the mass content of the above-mentioned cosmetic composition in the cosmetics is 0.025%.

[0012] A cosmetic comprising 0.01-0.03wt% of the above cosmetic composition.

[0013] Furthermore, the mass content of the above-mentioned cosmetic composition is 0.025%.

[0014] The present invention is beneficial in that: The invention discloses an antioxidant free radical cosmetic composition and its application, and cosmetics. The composition is composed of vitamin C, glutathione and tocopherol nicotinate, wherein tocopherol nicotinate is an ester of two vitamins, tocopherol and nicotinic acid, and not only has the physiological effects of tocopherol and nicotinic acid, but also optimizes the instability or irritation of each vitamin when used alone. The synergistic effect of the composition in improving the antioxidant capacity of the skin is studied through screening and optimization of the ratio of the three. The synergistic effect can be achieved through multiple mechanisms, including increasing the concentration of antioxidants in the skin, prolonging their active time, and playing a complementary role in different skin layers, thereby achieving the best synergistic antioxidant effect of the ingredients, giving full play to its skin care effect, and improving the scientific basis for its application in the formulation of skin care products. The composition has strong practicality and wide applicability. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a bar graph showing the antioxidant effects of each group of zebrafish.

[0016] Figure 2 The following are typical graphs of antioxidant activity of each experimental group. DETAILED DESCRIPTION

[0017] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] In the following examples, vitamin C is abbreviated as VC, glutathione is abbreviated as GSH, and tocopherol nicotinate is abbreviated as VEN.

[0019] A. Prepare the components according to the mass ratio in Table 1:

[0020] Table 1 B. Take 0.025g of each component in Table 1 above and prepare the sample set according to Table 2:

[0021] Table 2 C. Zebrafish antioxidant freedom test: C1. Experimental method: Zebrafish antioxidant test method, "Zebrafish antioxidant evaluation test standard operating procedures" C2. Test system and sample size: 1. Melanin allele mutant zebrafish (Albino) Zebrafish age: 2 days post fertilization (2dpf).

[0022] 2. Sample size for each experiment: 15 (N=10).

[0023] C3. Test principle: CellROX® reagent is a DNA dye with weak fluorescence. It can be oxidized by oxygen free radicals (ROS) in cells. The oxidation products bind to DNA and produce bright green fluorescence.

[0024] CellROX® is soluble in organic solvents (such as dimethyl sulfoxide), and the main component of zebrafish yolk sac is fat; therefore, CellROX® has a strong permeability in the yolk sac, and staining is obvious in this area.

[0025] Menadione can produce active oxygen free radicals. When the amount of free radicals produced is greater than the body's clearance capacity, an oxidative stress reaction occurs. The fluorescence intensity of the zebrafish yolk sac is used to evaluate whether the sample has an antioxidant effect.

[0026] C4. Test steps: 1. Randomly select zebrafish and place them in a 6-well plate, with 15 zebrafish in each well.

[0027] 2. The inducer menadione (concentration 1.5 μM / L) was administered in water to establish a zebrafish oxidative stress model (model control group), with a capacity of 3 mL per well.

[0028] 3. Establish a normal zebrafish control group in pure water with a volume of 3 mL per well.

[0029] 4. In each sample group in Table 2, a zebrafish sample group (A / B / C / D / E / F) was established, with a volume of 3 mL per well.

[0030] 5. Incubate at 28°C in dark for 22 hours.

[0031] 6. The zebrafish were stained with a specific ROS fluorescent reagent. After staining, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The data were analyzed and collected using advanced image processing software (Zebrafish Intelligent Image Analysis Software, Version 1.31). The fluorescence intensity (S) of the zebrafish yolk sac was analyzed and determined according to the following formula to determine whether the sample group had an antioxidant effect.

[0032] The normal control group, also known as the blank control, is a condition where no stress is established, in order to exclude the influence of the zebrafish itself.

[0033] The model control group was a zebrafish oxidative stress model established by adding the inducer menadione without adding any test samples.

[0034] 7. Judgment basis: Statistical analysis p<0.05, which is considered to be a significant difference.

[0035] C5. Test results As shown in Table 3 and Appendix Figure 1 As shown:

[0036] Table 3 From Table 3 above and attached Figure 1 It can be seen that sample groups AF all have the effect of resisting oxidative free radicals, but the effects vary greatly.

[0037] At the same dosage, compared with samples A, B, and C, sample D obtained by combining the three has a more significant and effective antioxidant free radical effect.

[0038] At the same dosage, the antioxidant effects of samples D, E, and F obtained by mixing VC, GSH, and VEN in different proportions are best in sample D, followed by sample E, but the antioxidant effect of sample F is significantly weaker than that of samples D and E, and weaker than that of samples A, B, and C. This indicates that the proportion of VC, GSH, and VEN that can achieve synergistic effects is within a certain range, and beyond this range, there is no systematic synergy.

[0039] C6. Mechanistic explanation of synergistic effect VC, GSH and VEN not only have the ability to resist oxidative free radicals themselves, but there is also a certain reducing effect between the three. For example, GSH can reduce the oxidized VC in the cytoplasm and continue to help VC continue to exert its antioxidant activity, while VEN will decompose into nicotinamide and tocopherol in the body. Tocopherol is an important antioxidant, and nicotinamide, as a precursor of NADH and NADPH, can significantly increase the content of NADPH, thereby reducing the oxidized GSH and exerting its antioxidant effect again.

[0040] D. Cosmetic preparation The cosmetics were prepared with component D, and the formula is shown in Table 4 below.

[0041]

[0042] Table 4

[0043] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the above embodiments do not limit the present invention in any form, and any technical solution obtained by equivalent replacement or equivalent transformation falls within the protection scope of the present invention.

Claims

1. A cosmetic composition for resisting oxidative free radicals, characterized in that: It includes vitamin C, glutathione and tocopherol nicotinate, and the mass ratio thereof is (20-35):(3-5):

1.

2. The cosmetic composition according to claim 1, characterized in that The mass ratio of vitamin C, glutathione and tocopherol nicotinate is 35:5:

1.

3. The cosmetic composition according to claim 1, characterized in that Used in the preparation of cosmetics.

4. The cosmetic composition according to claim 3, characterized in that The mass content of the cosmetic composition in cosmetics is 0.01-0.03%.

5. The cosmetic composition according to claim 4, characterized in that The mass content of the cosmetic composition in the cosmetics is 0.025%.

6. A cosmetic, characterized in that: A cosmetic composition comprising 0.01-0.03 wt % of the cosmetic composition according to any one of claims 1-4.

7. The cosmetic according to claim 6, characterized in that: The mass content of the cosmetic composition is 0.025%.

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