Multi-union bioreactor for adherent culture of cells
By setting up a rotation and culture mechanism in a multi-connected bioreactor where cells can be cultured adherently, the cultivation of multiple groups of cells under the same temperature and humidity environment is solved, and the problem that multiple cells cannot be cultured efficiently in the prior art is solved, and the improvement of the problem that various cells cannot be cultivated at the same time is improved. Cultivation efficiency.
Patent Information
- Application Number
- CN202411445690.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-16
- Publication Date
- 2024-12-24
AI Technical Summary
Existing multi-linked bioreactors that can be cultured adherently cannot culture multiple different cells in the same environment, resulting in inefficient culture.
By setting up a rotating mechanism and a culture mechanism, multiple groups of cells can be cultured under the same temperature and humidity environment, including heating mechanism, rotating mechanism, driving mechanism and fixing mechanism, ensuring that the cells are cultured under the same conditions.
The efficiency of cell culture is improved, allowing multiple groups of cells to be cultured under the same temperature and humidity environment, and the culture efficiency is improved.
Smart Images
Figure CN119177169A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cell culture, in particular to a multi-connected bioreactor in which cells can be cultured by adhering to a wall. Background Art
[0002] In recent decades, due to the rapid increase in human demand for biological products such as growth hormone, interferon, monoclonal antibodies, vaccines and interleukins, cell culture through large-scale in vitro culture technology is an effective method for producing biological products. The so-called large-scale cell culture technology refers to the technology of culturing animal cells in large quantities at high density in bioreactors under artificial conditions for the production of biological products. At present, the animal cells that can be cultured on a large scale include chicken embryos, pig kidneys, monkey kidneys, hamster kidneys and other primary cells and human diploid cells, CHO cells, BHK-rotating chambers, Vero cells, etc., and have successfully produced products including rabies vaccines, foot-and-mouth disease vaccines, hepatitis A vaccines, hepatitis B vaccines, erythropoietin, monoclonal antibodies, etc.
[0003] In the existing multi-connected bioreactors in which cells can be cultured adherently, for example, a multi-connected bioreactor in which cells can be cultured adherently is disclosed in the invention patent with application number 201711246119.7. This modular bioreactor suitable for culturing multiple cell types is used for large-scale high-density cell culture and target product expression in the biopharmaceutical industry. According to the different types of cultured cells, the relevant module components in the bioreactor are replaced to meet the culture requirements of different cell types.
[0004] However, during the cell culture process, it is impossible to culture a variety of different cells under the same environment, which reduces the culture efficiency. Summary of the invention
[0005] To solve the above technical problems, the present invention provides a multi-bioreactor for cell adherence culture, which is equipped with a rotating mechanism and a culture mechanism so that multiple groups of cells can be cultured in the same environment with the same temperature and humidity during the cell culture process, thereby improving the culture efficiency.
[0006] A multi-connected bioreactor for cell adherent culture of the present invention comprises a plurality of culture mechanisms; a heating mechanism, a rotating mechanism, a driving mechanism and a plurality of fixing mechanisms, wherein the rotating mechanism is mounted on the heating mechanism, the plurality of culture mechanisms are mounted on the rotating mechanism, the driving mechanism is mounted on the rotating mechanism, and the fixing mechanism is mounted on the rotating mechanism; The heating mechanism performs heating, the rotating mechanism performs rotation, the culturing mechanism performs culturing, the driving mechanism drives the culturing mechanism, and the fixing mechanism fixes the culturing mechanism; the culturing mechanism is fixed by the fixing mechanism, the culturing mechanism is rotated by opening the rotating mechanism, and the culturing mechanism is driven by the driving mechanism while the rotating mechanism rotates, thereby stirring the material, and the material is heated and kept warm by opening the heating mechanism, so that during the cell culturing process, multiple groups of cells can be cultured in the same environment with the same temperature and humidity, thereby improving the culturing efficiency.
[0007] Preferably, the heating mechanism includes a heating box, a heat preservation cover and a heating wire, the heat preservation cover is hingedly mounted on the upper end of the heating box, and the heating wire is installed in the heating box; the heating wire heats the heating box by energizing the heating wire, and the heat preservation cover keeps the heating box warm.
[0008] Preferably, the rotating mechanism includes a rotating bin and a motor. The rotating bin is rotatably installed in the heating box. The motor is installed in the heating box, and the output end of the motor is connected to the input end of the rotating bin. The rotating bin is driven to rotate by turning on the motor.
[0009] Preferably, the culture mechanism includes a culture tube, a bottom plate, a spring and a stirring blade. The culture tube is placed in a rotating bin, the bottom plate is slidably installed on the lower end of the culture tube through a spring, and the stirring blade is rotatably installed on the lower end of the bottom plate; the bottom plate is pressed against the spring to seal the inside of the culture tube, and the material is stirred by the rotation of the stirring blade.
[0010] Preferably, the driving mechanism includes a gear ring, multiple groups of gear shafts and multiple groups of clamps. The gear ring is installed in the heating box, and the multiple groups of gear shafts are rotatably installed on the rotating bin, and each group of gear shafts is meshed with the gear ring, and the output end of each group of gear shafts and the input end of each group of stirring blades are connected with a group of clamps; the gear shaft is driven to rotate by the rotation of the rotating bin, and the gear shaft rotates and meshes with the gear ring to cause the gear shaft to rotate. The gear shaft rotates and drives the clamp to rotate, and the stirring blade is driven to rotate by clamping the two groups of clamps.
[0011] Preferably, the fixing mechanism includes a protrusion, a clamping plate and a second spring, the protrusion is mounted on the culture tube, the clamping plate is rotatably mounted on the rotating bin, and the clamping plate is connected to the rotating bin via the second spring; the clamping plate is supported by the second spring so that the clamping plate clamps and fixes the protrusion.
[0012] Compared with the prior art, the present invention has the following beneficial effects: the culture mechanism is fixed by a fixing mechanism, the culture mechanism is rotated by opening a rotating mechanism, the culture mechanism is driven by a driving mechanism while the rotating mechanism rotates, thereby stirring the material, and the material is heated and kept warm by opening a heating mechanism, so that during the cell culture process, multiple groups of cells can be cultured in the same environment with the same temperature and humidity, thereby improving the culture efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 It is a schematic diagram of the axonometric structure of the present invention; Figure 2 It is a right-side sectional axonometric structural schematic diagram of the present invention; Figure 3 It is a schematic diagram of the axonometric structure of the present invention in top view; Figure 4 The fixing mechanism of the present invention is Figure 2 A right-view axonometric structural diagram of the middle A section; Figure 5 The culture mechanism of the present invention is Figure 2 A schematic diagram of the axonometric structure of the right-view section of the middle B part; Markings in the attached drawings: 1. heating mechanism; 11. heating box; 12. insulation cover; 13. heating wire; 2. rotating mechanism; 21. rotating bin; 22. motor; 3. culture mechanism; 31. culture tube; 32. bottom plate; 33. spring one; 34. stirring blade; 4. driving mechanism; 41. gear ring; 42. gear shaft; 43. clamping block; 5. fixing mechanism; 51. protrusion; 52. clamping plate; 53. spring two. DETAILED DESCRIPTION
[0014] In order to facilitate understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive. Example 1
[0015] like Figures 1 to 5 As shown, a multi-connected bioreactor in which cells can be cultured adherently comprises a plurality of culture mechanisms 3, a heating mechanism 1, a rotating mechanism 2, a driving mechanism 4 and a plurality of fixing mechanisms 5, wherein the rotating mechanism 2 is mounted on the heating mechanism 1, the plurality of culture mechanisms 3 are mounted on the rotating mechanism 2, the driving mechanism 4 is mounted on the rotating mechanism 2, and the fixing mechanism 5 is mounted on the rotating mechanism 2; The heating mechanism 1 performs heating, the rotating mechanism 2 performs rotation, the cultivating mechanism 3 performs cultivating, the driving mechanism 4 drives the cultivating mechanism 3, and the fixing mechanism 5 fixes the cultivating mechanism 3; The heating mechanism 1 comprises a heating box 11, a heat preservation cover 12 and a heating wire 13. The heat preservation cover 12 is hingedly mounted on the upper end of the heating box 11, and the heating wire 13 is installed in the heating box 11; The rotating mechanism 2 includes a rotating chamber 21 and a motor 22. The rotating chamber 21 is rotatably mounted in the heating box 11. The motor 22 is mounted in the heating box 11, and the output end of the motor 22 is connected to the input end of the rotating chamber 21. The culture mechanism 3 includes a culture tube 31, a bottom plate 32, a spring 33 and a stirring blade 34. The culture tube 31 is placed in the rotating bin 21. The bottom plate 32 is slidably mounted on the lower end of the culture tube 31 through the spring 33. The stirring blade 34 is rotatably mounted on the lower end of the bottom plate 32. The driving mechanism 4 includes a gear ring 41, multiple sets of gear shafts 42 and multiple sets of clamping blocks 43. The gear ring 41 is installed in the heating box 11, and the multiple sets of gear shafts 42 are rotatably installed on the rotating bin 21, and each set of gear shafts 42 is meshed with the gear ring 41, and the output end of each set of gear shafts 42 and the input end of each set of stirring blades 34 are connected to a set of clamping blocks 43; The fixing mechanism 5 includes a protrusion 51, a clamping plate 52 and a second spring 53. The protrusion 51 is mounted on the culture tube 31, the clamping plate 52 is rotatably mounted on the rotating bin 21, and the clamping plate 52 is connected to the rotating bin 21 through the second spring 53. The clamping plate 52 is supported by the spring 2 53 so that the clamping plate 52 clamps and fixes the protrusion 51, and the rotating bin 21 is driven by turning on the motor 22 to rotate the rotating bin 21, and the rotation of the rotating bin 21 drives the gear shaft 42 to rotate, and the gear shaft 42 meshes with the gear ring 41 while rotating to make the gear shaft 42 rotate, and the block 43 is driven to rotate while the gear shaft 42 rotates, and the stirring blade 34 is driven to rotate by clamping the two groups of blocks 43, and the stirring blade 34 is rotated, and the bottom plate 32 is pressed by the spring 1 33 to seal the inside of the culture tube 31, and the material is stirred by the rotation of the stirring blade 34, and at the same time, the heating wire 13 is energized to heat the inside of the heating box 11, and the heating box 11 is kept warm by the insulation cover 12, so that during the cell culture process, multiple groups of cells can be cultured in the same environment with the same temperature and humidity, thereby improving the culture efficiency.
[0016] like Figures 1 to 5As shown, a multi-connected bioreactor in which cells can be cultured adherently to the wall of the present invention, when working, the clamping plate 52 is supported by the spring 2 53 so that the clamping plate 52 clamps and fixes the protrusion 51, and the rotating bin 21 is driven by turning on the motor 22 to rotate the rotating bin 21, and the gear shaft 42 is rotated by the rotation of the rotating bin 21. While the gear shaft 42 rotates, it meshes with the gear ring 41 to make the gear shaft 42 rotate, and while the gear shaft 42 rotates, it drives the clamping block 43 to rotate, and the stirring blade 34 is driven to rotate by clamping the two groups of clamping blocks 43, and the stirring blade 34 is rotated, and the bottom plate 32 is pressed by the spring 1 33 to seal the inside of the culture tube 31, and the material is stirred by the rotation of the stirring blade 34, and at the same time, the heating wire 13 is energized to heat the inside of the heating box 11, and the heating box 11 is kept warm by the insulation cover 12.
[0017] The motor 22 of the present invention is purchased on the market, and technicians in the industry only need to install and operate it according to the accompanying instruction manual, without the need for technicians in the field to make creative efforts.
[0018] The main function achieved by the present invention is: during the cell culture process, by setting a rotating mechanism and a culture mechanism, multiple groups of cells can be cultured in the same environment with the same temperature and humidity during the cell culture process, thereby improving the culture efficiency.
[0019] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A multi-connected bioreactor for cell adherent culture, comprising a plurality of culture mechanisms (3); characterized in that: It also includes a heating mechanism (1), a rotating mechanism (2), a driving mechanism (4) and a plurality of fixing mechanisms (5), wherein the rotating mechanism (2) is mounted on the heating mechanism (1), the plurality of culture mechanisms (3) are mounted on the rotating mechanism (2), the driving mechanism (4) is mounted on the rotating mechanism (2), and the fixing mechanism (5) is mounted on the rotating mechanism (2); The heating mechanism (1) performs heating, the rotating mechanism (2) performs rotation, the cultivating mechanism (3) performs cultivating, the driving mechanism (4) drives the cultivating mechanism (3), and the fixing mechanism (5) fixes the cultivating mechanism (3).
2. A multiple bioreactor for cell adherent culture as claimed in claim 1, characterized in that: The heating mechanism (1) comprises a heating box (11), a heat-insulating cover (12) and a heating wire (13); the heat-insulating cover (12) is hingedly mounted on the upper end of the heating box (11), and the heating wire (13) is mounted inside the heating box (11).
3. A multiple bioreactor for cell adherent culture as claimed in claim 2, characterized in that: The rotating mechanism (2) comprises a rotating bin (21) and a motor (22); the rotating bin (21) is rotatably mounted in the heating box (11); the motor (22) is mounted in the heating box (11); and the output end of the motor (22) is connected to the input end of the rotating bin (21).
4. A multiple bioreactor for cell adherent culture as claimed in claim 3, characterized in that: The culture mechanism (3) comprises a culture tube (31), a bottom plate (32), a spring (33) and a stirring blade (34); the culture tube (31) is placed in the rotating bin (21); the bottom plate (32) is slidably mounted on the lower end of the culture tube (31) via the spring (33); and the stirring blade (34) is rotatably mounted on the lower end of the bottom plate (32).
5. The multiple bioreactor for cell adherent culture according to claim 3, characterized in that: The driving mechanism (4) comprises a gear ring (41), a plurality of sets of gear shafts (42) and a plurality of sets of clamping blocks (43); the gear ring (41) is mounted in the heating box (11); the plurality of sets of gear shafts (42) are rotatably mounted on the rotating bin (21); each set of gear shafts (42) is meshed with the gear ring (41); and the output end of each set of gear shafts (42) and the input end of each set of stirring blades (34) are connected to a set of clamping blocks (43).
6. The multiple bioreactor for cell adherent culture according to claim 4, characterized in that: The fixing mechanism (5) comprises a protrusion (51), a clamping plate (52) and a second spring (53); the protrusion (51) is mounted on the culture tube (31); the clamping plate (52) is rotatably mounted on the rotating bin (21); and the clamping plate (52) is connected to the rotating bin (21) via the second spring (53).