Method for increasing deep inert organic carbon in soil based on deep-habitat earthworms

Abstract

The invention provides a method for increasing deep inert organic carbon in soil based on deep-dwelling earthworms, which comprises the following steps: carrying out ground weeding and land leveling treatment on a land parcel to obtain a pretreated land parcel; and performing field irrigation on the pretreated land parcel to obtain an irrigated land parcel. Deep-dwelling earthworms are put on the soil surface of the irrigated land parcel, and the total organic carbon content and the inert organic carbon content of the soil are increased through the deep-dwelling earthworms. Soil carbon sequestration result analysis shows that the soil organic carbon reserve is increased by 21.0% and the soil inert organic carbon reserve is increased by 29.3% when the deep-dwelling earthworms are independently used for improving the plot soil. The core of carbon sequestration is to increase the content of inert organic carbon in the soil of 40-80cm underground soil layer, and compared with active organic carbon, the inert organic carbon has longer solid storage time in the soil. In addition, the deep soil is not easy to disturb, and the preservation time of the organic carbon in the soil is further prolonged. The method also has remarkable environmental friendliness, and effective utilization of wastes can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of biological carbon fixation, and in particular to a method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms. Background Art

[0002] As an important means to mitigate global climate change, soil carbon sequestration technology has attracted increasing attention for its application and development. However, the current soil carbon sequestration strategy has encountered some problems in practice, which are mainly reflected in the depth of carbon sequestration, organic carbon activity and economic benefits.

[0003] First, from the perspective of carbon sequestration depth, most of the current soil carbon sequestration technologies are mainly concentrated in the 0-30cm soil surface layer. Although carbon sequestration in this soil layer is easy to operate, the soil is disturbed by natural and human factors such as farming and soil erosion, resulting in poor carbon sequestration effects. In contrast, deep soil layers of 40-80cm have good carbon sequestration effects due to their stability and less disturbed environmental conditions. Therefore, future soil carbon sequestration technologies should pay more attention to the carbon sequestration potential of deep soils to improve the stability and durability of carbon sequestration.

[0004] Secondly, from the perspective of the activity characteristics of organic carbon in carbon fixation, although soil carbon fixation technologies such as returning organic matter to the field can significantly increase the content of soil organic carbon, the content of active components in these organic carbons is extremely high. This means that although these active organic carbons can rapidly increase soil carbon reserves in the short term, in the long term, due to the easy decomposition of active organic carbon, the carbon fixation effect of active organic carbon is not ideal. Therefore, future soil carbon fixation technologies should turn to more stable inert organic carbon fixation to achieve long-term carbon fixation effects.

[0005] In summary, future soil carbon sequestration technology needs to balance and optimize the three aspects of soil depth, organic carbon activity and economic benefits. By exploring deep soil carbon sequestration, inert organic carbon sequestration and economically beneficial carbon sequestration models, the important role of soil carbon sequestration in mitigating global climate change can be better played. Summary of the invention

[0006] In order to overcome the problems existing in the related art, the purpose of the present invention is to provide a method for increasing the inert organic carbon in the deep soil layer based on deep-dwelling earthworms, which can increase the inert organic carbon content in the 40-80 cm soil layer underground. Inert organic carbon is an ideal carbon fixation material and can effectively improve the carbon storage capacity of the soil. In addition, the deep soil is not easily disturbed, which is conducive to further extending the storage time of organic carbon in the soil.

[0007] Methods for increasing inert organic carbon in the deep soil layer based on deep-dwelling earthworms include:

[0008] Weeding and leveling the land on the plot to obtain a pre-treated plot;

[0009] Performing field irrigation on the pretreated plot to obtain an irrigated plot;

[0010] Deep-dwelling earthworms are placed on the soil surface of the irrigated plot, and the total organic carbon content and inert organic carbon content of the soil are increased by the deep-dwelling earthworms.

[0011] In a preferred technical solution of the present invention, before placing deep-dwelling earthworms on the soil surface of the irrigated land, the method further comprises:

[0012] Dividing the irrigated plot into N rows, where N≥2;

[0013] Honeysuckle trees were transplanted into the area corresponding to the N rows of the irrigated plot.

[0014] In a preferred technical solution of the present invention, the step of transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot includes:

[0015] Set the planting row spacing of honeysuckle trees to 2 meters;

[0016] Set the column spacing of honeysuckle trees in the same row to 2 meters;

[0017] Each honeysuckle tree occupies an area of ​​4 square meters;

[0018] A plurality of honeysuckle trees are planted in the irrigated plot according to the planting row spacing, the column spacing and the floor area.

[0019] In a preferred technical solution of the present invention, the step of placing deep-dwelling earthworms on the soil surface of the irrigated plot includes:

[0020] The earthworms were placed on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the placement environment is a seedling planting temperature less than or equal to 25°C, and the light intensity less than or equal to 5000lux.

[0021] In a preferred technical solution of the present invention, the method of irrigating the pretreated plots in the field to obtain irrigated plots includes:

[0022] The pretreated plots were irrigated and sprayed for 7 consecutive days to maintain 70% of the field water capacity to obtain irrigated plots.

[0023] In a preferred technical solution of the present invention, after increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises:

[0024] Use a soil drill to collect the soil layer 0-80 cm below the ground after the deep-dwelling earthworms are released;

[0025] Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm;

[0026] The total organic carbon content and inert organic carbon content in the soil were tested layer by layer.

[0027] In a preferred technical solution of the present invention, after obtaining the irrigated land, the method further comprises:

[0028] A water channel is set around the irrigated plot as a barrier to prevent the deep-dwelling earthworms from escaping;

[0029] A sprinkler belt is installed on the irrigated plot, and the sprinkler belt is used for daily watering.

[0030] In a preferred technical solution of the present invention, the ridge height of the water channel is 20 cm, and the single hole flow rate of the sprinkler belt is 2 L / h.

[0031] In a preferred technical solution of the present invention, before placing deep-dwelling earthworms on the soil surface of the irrigated land, the method further comprises:

[0032] The electric shock method is used to collect mature earthworms with rings; the input voltage of the earthworm trap used in the electric shock method is 12V and the output voltage is 3600V.

[0033] In a preferred technical solution of the present invention, after placing deep-dwelling earthworms on the soil surface of the irrigated land, the method further comprises:

[0034] After the deep-dwelling earthworms were placed, the plots were watered every 15 days;

[0035] Detecting the field water holding capacity of the soil, and if the field water holding capacity of the soil is equal to 100%, stopping watering;

[0036] Fertilizer should be applied every 7-15 days.

[0037] The beneficial effects of the present invention are:

[0038] The method for increasing the deep inert organic carbon in the soil based on deep-dwelling earthworms provided by the present invention includes weeding the ground and leveling the land to obtain a pre-treated plot. The pre-treated plot is irrigated in the field to obtain an irrigated plot. Deep-dwelling earthworms are placed on the soil surface of the irrigated plot, and the total organic carbon content and the inert organic carbon content in the soil are increased by deep-dwelling earthworms. Analysis of the results of soil carbon fixation showed that the soil of the plot was improved by deep-dwelling earthworms alone, and the soil organic carbon reserves increased by 21.0%, and the soil inert organic carbon reserves increased by 29.3%. The core of carbon fixation in the present invention is to increase the content of soil inert organic carbon in the 40-80cm soil layer underground. Compared with active organic carbon, inert organic carbon is more difficult to be eaten and decomposed by soil animals and microorganisms, and the sequestration time in the soil is longer, and can even reach 2-11 times that of active organic carbon. This characteristic makes inert organic carbon an ideal carbon fixation material, which can effectively improve the carbon storage capacity of the soil. In addition, deep soil is not easily disturbed, further extending the storage time of organic carbon in the soil. The present invention significantly prolongs the storage time of organic carbon in the soil and is also significantly environmentally friendly. During implementation, the soil in which deep-dwelling earthworms are placed can absorb agricultural waste such as cow dung, thereby achieving effective utilization of waste. This not only helps to reduce carbon emissions from agricultural waste and reduce environmental pollution, but also provides an effective waste treatment method for agricultural production, thereby promoting sustainable agricultural development. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic diagram of the present invention for achieving deep soil carbon fixation through deep-dwelling earthworms and honeysuckle trees;

[0040] Figure 2 This is a flow chart of the present invention for realizing deep soil carbon fixation using deep-dwelling earthworms alone;

[0041] Figure 3 This is a flow chart of the present invention for combining deep-dwelling earthworms and honeysuckle trees to achieve deep soil carbon fixation;

[0042] Figure 4 is the total organic carbon content of soil at different depths of the present invention;

[0043] Figure 5 is the soil inert organic carbon content of soil layers at different depths of the present invention;

[0044] Figure 6 It is a regression analysis diagram of the soil inert organic carbon content and the soil total organic carbon content of the present invention;

[0045] Figure 7 is a graph showing the experimental results of soil total organic carbon storage in different plots of the present invention;

[0046] Figure 8It is a graph showing the experimental results of soil inert organic carbon storage in different plots of land according to the present invention. DETAILED DESCRIPTION

[0047] The preferred embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the preferred embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0048] Example 1

[0049] like Figure 2 As shown, this embodiment provides a method for increasing the inert organic carbon in the deep soil layer based on deep-dwelling earthworms, comprising:

[0050] S1: Weeding and leveling the land to obtain a pre-treated land.

[0051] S2: performing field irrigation on the pretreated plot to obtain an irrigated plot.

[0052] S3: placing deep-dwelling earthworms on the soil surface of the irrigated plot, and increasing the total organic carbon content and inert organic carbon content of the soil through the deep-dwelling earthworms.

[0053] The plot is weeded and leveled to make the plot flat, and the pre-treated plot is irrigated and sprayed for 7 consecutive days to maintain 70% of the field water holding capacity to obtain an irrigated plot. The ground flatness is not higher than 5 cm, and a canal is set around the irrigated plot as an isolation belt to prevent deep-dwelling earthworms from escaping. A sprinkler belt is installed on the irrigated plot, and the sprinkler belt is used for daily watering. The ridge height of the canal is 20 cm, and the single hole flow rate of the sprinkler belt is 2 L / h.

[0054] The step of placing deep-dwelling earthworms on the soil surface of the irrigated plot comprises:

[0055] The earthworms were placed on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the placement environment is a seedling planting temperature less than or equal to 25°C, and the light intensity less than or equal to 5000lux.

[0056] Preferably, the deep-dwelling earthworm is the ginseng earthworm, and adult earthworms with reproductive rings and body color ranging from brown to purple-gray are used for testing, and old species with black body color are not used. The diet of deep-dwelling earthworms is mainly herbivorous and supplemented by soil diet. They live in the soil at a depth of 40-80cm underground, and dig vertical caves to the surface at night to feed on organic matter. In order to achieve long-term carbon fixation, earthworms need to be able to reproduce. The reproductive ring means that the earthworms are sexually mature and can mate and reproduce, so the ginseng earthworm with a reproductive ring is used. On the one hand, the ginseng earthworm can migrate above-ground organic matter to deep soil, thereby achieving deep soil carbon fixation. On the other hand, the ginseng earthworm feeds on organic matter and wraps its excrement in soil aggregates to increase the inert organic carbon in the soil.

[0057] In order to achieve long-term carbon fixation, earthworms need to reproduce continuously. The reproductive ring means that the earthworms are sexually mature and can mate and reproduce. The selection of earthworms has the following advantages: on the one hand, earthworms can transfer above-ground organic matter to deep soil, thereby achieving deep soil carbon fixation. On the other hand, earthworms feed on organic matter and wrap their excrement in soil aggregates, thereby increasing the inert organic carbon in the soil.

[0058] Before placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises:

[0059] The electric shock method is used to collect mature earthworms with rings; the input voltage of the earthworm trap used in the electric shock method is 12V and the output voltage is 3600V.

[0060] In September every year, a 12V input voltage and 3600V output voltage earthworm trap is used to collect mature earthworms with obvious rings. Some of the earthworms are applied to the soil surface of the irrigated plots, and the remaining earthworms are cleaned and dried to prepare dried earthworm medicinal materials.

[0061] After placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises:

[0062] After the deep-dwelling earthworms were placed, the plots were watered every 15 days;

[0063] Detecting the field water holding capacity of the soil, and if the field water holding capacity of the soil is equal to 100%, stopping watering;

[0064] Fertilizer should be applied every 7-15 days.

[0065] The fertilizer in this embodiment is cow dung, and the amount of cow dung placed is 4kg / m 2 / moon.

[0066] After increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises:

[0067] S41: Use a soil auger to collect the soil layer of 0-80 cm underground in the plot after the deep-dwelling earthworms are released.

[0068] S42: Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm.

[0069] S43: Detect the total organic carbon content and inert organic carbon content in the soil layer by layer.

[0070] like Figure 4 As shown, for the earthworm plots that used deep-dwelling earthworms alone to achieve deep soil carbon fixation, the total soil organic carbon content in the soil layer with a depth of 0-20 cm was 8.3 g / kg. In comparison, the total soil organic carbon content of the blank plots that did not apply deep-dwelling earthworms was 4.2 g / kg. The total soil organic carbon content of the earthworm plots was twice that of the blank plots.

[0071] like Figure 5 As shown, for the earthworm plots that use deep-dwelling earthworms alone to achieve deep soil carbon fixation, the soil inert organic carbon content of the soil layer with a depth of 0-20 cm is 5g / kg. In comparison, the soil inert organic carbon content of the blank plots without deep-dwelling earthworms is 2.8g / kg. In this depth range, the soil inert organic carbon content of the earthworm plots is 78.6% higher than that of the blank plots. For the earthworm plots that use deep-dwelling earthworms alone to achieve deep soil carbon fixation, the soil inert organic carbon content of the soil with a depth of 20-40 cm is 5.6g / kg. In comparison, the soil inert organic carbon content of the blank plots without deep-dwelling earthworms is 3.4g / kg. In this depth range, the soil inert organic carbon content of the earthworm plots is 64.7% higher than that of the blank plots. For the earthworm plots that used deep-dwelling earthworms alone to achieve deep soil carbon fixation, the soil inert organic carbon content of the soil at a depth of 40-60 cm was 2.5 g / kg. In comparison, the soil inert organic carbon content of the blank plots that did not apply deep-dwelling earthworms was 2.3 g / kg. Within this depth range, the soil inert organic carbon content of the earthworm plots was 8.7% higher than that of the blank plots.

[0072] like Figure 6 As shown in the figure, the total organic carbon in the soil increases with the increase of inert organic carbon. The inert organic carbon content in the soil is taken as the independent variable x, and the total organic carbon content in the soil is taken as the dependent variable y. The relationship between y and x satisfies the following: y = 1.4072x-0.1035, and the determination coefficient R 2 It is 0.8854. The determination coefficient indicates how well the model fits the data. The larger the determination coefficient, the better the simulation fits the data.

[0073] like Figure 7 As shown in Figure 2, the total soil organic carbon storage in the deep-dwelling earthworm plot alone increased by 21.0%. Figure 8 As shown, the soil inert organic carbon storage in the deep-dwelling earthworm plot alone increased by 29.3%.

[0074] The method of increasing the inert organic carbon in the deep soil layer based on deep-dwelling earthworms of the present invention is not only low in implementation cost, but also environmentally friendly and can bring significant economic benefits. The main purpose of using the above method is to avoid artificial or natural disturbances that accelerate the decomposition and mineralization of organic carbon, and to significantly prolong the storage time of organic carbon in the soil in view of the fact that active organic carbon is not easy to preserve in the soil.

[0075] The method of increasing the inert organic carbon in the deep soil layer based on deep-dwelling earthworms in this embodiment has broad application prospects and is environmentally friendly while fixing carbon, thereby providing a new solution for addressing global climate change and promoting sustainable agricultural development.

[0076] The method for increasing the deep inert organic carbon in the soil based on deep-dwelling earthworms provided in this embodiment includes weeding the ground and leveling the land to obtain a pre-treated plot. The pre-treated plot is irrigated in the field to obtain an irrigated plot. Deep-dwelling earthworms are placed on the soil surface of the irrigated plot to increase the total organic carbon content and the inert organic carbon content of the soil by deep-dwelling earthworms. Analysis of the results of soil carbon fixation showed that the soil of the plot was improved by deep-dwelling earthworms alone, and the soil organic carbon reserves increased by 21.0%, and the soil inert organic carbon reserves increased by 29.3%. The core of carbon fixation in the present invention is to increase the content of soil inert organic carbon in the 40-80cm soil layer underground. Compared with active organic carbon, inert organic carbon is more difficult to be decomposed by soil animals and microorganisms, and the sequestration time in the soil is longer, and can even reach 2-11 times that of active organic carbon. This characteristic makes inert organic carbon an ideal carbon fixation material, which can effectively improve the carbon storage capacity of the soil. In addition, deep soil is not easily disturbed, which further prolongs the storage time of organic carbon in the soil. The present invention significantly prolongs the storage time of organic carbon in the soil and is also significantly environmentally friendly. During implementation, the soil in which deep-dwelling earthworms are placed can absorb agricultural waste such as cow dung, thereby achieving effective utilization of waste. This not only helps to reduce carbon emissions from agricultural waste and reduce environmental pollution, but also provides an effective waste treatment method for agricultural production, thereby promoting sustainable agricultural development.

[0077] Example 2

[0078] like Figure 3 As shown, this embodiment provides a method for increasing the inert organic carbon in the deep soil layer based on deep-dwelling earthworms, comprising:

[0079] S1: Weeding and leveling the land to obtain a pre-treated land.

[0080] S2: performing field irrigation on the pretreated plot to obtain an irrigated plot.

[0081] S3: Divide the irrigated plot into N rows, where N≥2.

[0082] S4: Transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot.

[0083] S5: placing deep-dwelling earthworms onto the soil surface of the irrigated plot, and increasing the total organic carbon content and inert organic carbon content of the soil through the deep-dwelling earthworms.

[0084] The step of transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot comprises:

[0085] S41: Set the planting row spacing of honeysuckle trees to 2 meters.

[0086] S42: Set the column spacing of honeysuckle trees in the same row to 2 meters.

[0087] S43: Each honeysuckle tree occupies an area of ​​4 square meters.

[0088] S44: Planting a plurality of honeysuckle trees in the irrigated plot according to the planting row spacing, the column spacing and the land area.

[0089] like Figure 1 As shown, the row spacing and column spacing of the honeysuckle trees are both 2 meters, the height of the honeysuckle trees is greater than 1 meter, the shade area is greater than 0.5 square meters, and the planting density is one tree per 4 square meters. Honeysuckle is collected manually once in May every year, the collected honeysuckle is dried, and then the dried honeysuckle is used as a tea material. The ginseng-shaped far-blind earthworms with mature rings are collected once in September every year, some of the ginseng-shaped far-blind earthworms are applied to the soil surface, and the other part of the ginseng-shaped far-blind earthworms are dried to clean the intestines and prepare dry earthworm medicinal materials.

[0090] The step of placing deep-dwelling earthworms on the soil surface of the irrigated plot comprises:

[0091] The earthworms were placed on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the placement environment is a seedling planting temperature less than or equal to 25°C, and the light intensity less than or equal to 5000lux.

[0092] After increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises:

[0093] S61: Use a soil auger to collect the soil layer of 0-80 cm underground in the plot after the deep-dwelling earthworms are released.

[0094] S62: Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm.

[0095] S63: Detect the total organic carbon content and inert organic carbon content in the soil layer by layer.

[0096] like Figure 4 As shown in the figure, by breeding deep-dwelling earthworms and planting honeysuckle, the total organic carbon content in the deep soil layer of 40-80 cm increased by 12.8-14.6%, and the organic carbon content in all soil layers increased by 9.31%-105.5%. Figure 5 As shown in the figure, by breeding deep-dwelling earthworms and planting honeysuckle, the inert organic carbon content in the deep soil layer of 40-80 cm increased by 13.8-22.1%, and the organic carbon content in all soil layers increased by 13.8-94.1%. Figure 6 As shown in Figure 2, total organic carbon in soil increases with increasing inert organic carbon. Figure 7 As shown in the study, soil organic carbon storage increased by 36.2% when deep-dwelling earthworms were cultivated and honeysuckle trees were planted. Figure 8 As shown, breeding deep-dwelling earthworms and planting honeysuckle trees increased the soil inert organic carbon storage by 31.2%.

[0097] In this embodiment, deep-dwelling earthworms are applied to the soil surface on the basis of planting honeysuckle trees. The litter and root secretions of the honeysuckle trees increase the input of exogenous organic matter, thereby improving the content of soil organic carbon as a whole. Figure 5 When the soil depth is 0-20cm and 20-40cm, the soil inert organic carbon content of the plots improved with earthworms alone is higher than that of the plots improved with a combination of earthworms and honeysuckle. The reason is that the soil layers corresponding to the above two soil depth ranges are the main distribution areas of the roots of honeysuckle trees, and the rich rhizosphere microorganisms have a significant activation effect on soil organic carbon.

[0098] It should be noted that, in this article, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, device, article or method including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, device, article or method. In the absence of further restrictions, an element defined by the sentence "includes a ..." does not exclude the presence of other identical elements in the process, device, article or method including the element.

[0099] The above description is only a preferred embodiment of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms, characterized in that: include: Weeding and leveling the land on the plot to obtain a pre-treated plot; Performing field irrigation on the pretreated plot to obtain an irrigated plot; Deep-dwelling earthworms are placed on the soil surface of the irrigated plot, and the total organic carbon content and inert organic carbon content of the soil are increased by the deep-dwelling earthworms.

2. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: Before placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises: Dividing the irrigated plot into N rows, where N≥2; Honeysuckle trees were transplanted into the area corresponding to the N rows of the irrigated plot.

3. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 2, characterized in that: The step of transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot comprises: Set the planting row spacing of honeysuckle trees to 2 meters; Set the column spacing of honeysuckle trees in the same row to 2 meters; Each honeysuckle tree occupies an area of ​​4 square meters; A plurality of honeysuckle trees are planted in the irrigated plot according to the planting row spacing, the column spacing and the floor area.

4. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: The step of placing deep-dwelling earthworms on the soil surface of the irrigated plot comprises: The earthworms were placed on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the placement environment is a seedling planting temperature less than or equal to 25°C, and the light intensity less than or equal to 5000lux.

5. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: The method of performing field irrigation on the pretreated plot to obtain an irrigated plot comprises: The pretreated plots were irrigated and sprayed for 7 consecutive days to maintain 70% of the field water capacity to obtain irrigated plots.

6. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: After increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises: Use a soil drill to collect the soil layer 0-80 cm below the ground after the deep-dwelling earthworms are released; Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm; The total organic carbon content and inert organic carbon content in the soil were tested layer by layer.

7. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: After obtaining the irrigated land, the method further comprises: A water channel is set around the irrigated plot as a barrier to prevent the deep-dwelling earthworms from escaping; A sprinkler belt is installed on the irrigated plot, and the sprinkler belt is used for daily watering.

8. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 7, characterized in that: The ridge height of the water channel is 20 cm, and the single hole flow rate of the sprinkler belt is 2 L / h.

9. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: Before placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises: The electric shock method is used to collect mature earthworms with rings; the input voltage of the earthworm trap used in the electric shock method is 12V and the output voltage is 3600V.

10. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: After placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises: After the deep-dwelling earthworms were placed, the plots were watered every 15 days; Detecting the field water holding capacity of the soil, and if the field water holding capacity of the soil is equal to 100%, stopping watering; Fertilizer should be applied every 7-15 days.

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