Culture medium for improving survival rate of lucid ganoderma and application thereof

Abstract

The invention discloses a culture medium for increasing the survival rate of ganoderma lucidum and application thereof, and belongs to the technical field of edible fungus cultivation. The culture medium comprises the following components in parts by weight: 65-70 parts of corn straws, 10-15 parts of peanut shells, 10-15 parts of lucid ganoderma residues, 1-2 parts of gypsum powder, 1-2 parts of daminozide, 0.5-1 part of phenylalanine and 1-2 parts of light calcium carbonate, and the water content is 55-65%. According to the culture medium, the corn straw, the peanut shells and the lucid ganoderma residues are used for replacing wood to serve as a substrate, waste of tree resources is avoided, the crop shells, the crop straw and the lucid ganoderma residues can be fully utilized, adverse effects on the environment are avoided, and waste of the resources is further avoided. Meanwhile, the daminozide and the phenylalanine are added to provide nutrition for the ganoderma lucidum and promote growth, the seedling emergence number, the dry weight of thalli and the survival rate of the ganoderma lucidum are remarkably increased, and the survival rate can reach 100%.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungus cultivation, in particular to a culture medium for improving the survival rate of ganoderma lucidum and an application thereof. Background Art

[0002] Ganoderma lucidum is a traditional precious Chinese medicinal material. It is a basidiomycete, Polyporaceae, and is known as the "immortal grass". Its appearance is umbrella-shaped, semicircular or nearly circular. The main active ingredients of Ganoderma lucidum include Ganoderma polysaccharides, triterpenoids, alkaloids, nucleosides, etc. It has multiple pharmacological effects such as anti-tumor, anti-oxidation, immunomodulation, and hypoglycemic. As people understand the health benefits of Ganoderma lucidum, the market demand for Ganoderma lucidum products has increased, and the field cultivation area of ​​Ganoderma lucidum has also been expanding.

[0003] The traditional culture medium formula used for artificial cultivation of Ganoderma lucidum is: 75% sawdust, 20% wheat bran, 3% corn flour, 1% gypsum powder, and 1% light calcium carbonate. Some people also use logs or logs instead of sawdust for cultivation. These cultivation methods all use sawdust as the main raw material, which has a large demand for wood and destroys forest resources. Based on this, there is an urgent need to provide a new culture medium that does not contain sawdust in the raw materials, so as to improve the survival rate of Ganoderma lucidum while protecting resources and reducing costs. Summary of the invention

[0004] The purpose of the present invention is to provide a culture medium for improving the survival rate of Ganoderma lucidum and its application, so as to solve the problems existing in the above-mentioned prior art. The culture medium provided by the present invention significantly improves the number of Ganoderma lucidum seedlings, dry weight of fungi and survival rate, and has great application value.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The invention provides a culture medium for improving the survival rate of ganoderma lucidum, comprising the following components by weight: 65-70 parts of corn stalks, 10-15 parts of peanut shells, 10-15 parts of ganoderma lucidum residues, 1-2 parts of gypsum powder, 1-2 parts of butyrylhydrazide, 0.5-1 parts of phenylalanine and 1-2 parts of light calcium carbonate.

[0007] Furthermore, the water content of the culture medium is 55-65%.

[0008] The present invention also provides a method for preparing the culture medium, comprising the following steps:

[0009] The corn stalks, peanut shells and ganoderma lucidum residues are crushed, and then uniformly mixed with gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain a culture medium;

[0010] The culture medium is put into a bag and sterilized.

[0011] Furthermore, the corn stalks, peanut shells and ganoderma lucidum residues are crushed to 20 meshes.

[0012] Furthermore, the water content of the culture medium is 55-65%.

[0013] Furthermore, the sterilization condition is 121° C. for 4-5 hours.

[0014] The invention also provides application of the culture medium in the cultivation of ganoderma lucidum.

[0015] The invention also provides application of the culture medium in improving the survival rate of ganoderma lucidum.

[0016] The present invention discloses the following technical effects:

[0017] The culture medium for improving the survival rate of ganoderma lucidum provided by the present invention uses corn stalks, peanut shells and ganoderma lucidum residues instead of wood as a substrate, which not only avoids the waste of tree resources, but also can make full use of crop shells, crop stalks and ganoderma lucidum residues without discarding them, thereby avoiding adverse effects on the environment and further avoiding waste of resources. At the same time, daminozide and phenylalanine are added to provide nutrition for ganoderma lucidum, promote growth, significantly improve the number of ganoderma lucidum seedlings, dry weight of bacteria and survival rate, and the survival rate can reach 100%.

[0018] The culture medium for improving the survival rate of ganoderma lucidum provided by the invention has simple and easy-to-obtain raw materials and a simple production process. DETAILED DESCRIPTION

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0021] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0022] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0023] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0024] Daminozide (CAS: 1596-84-5), phenylalanine (CAS: 673-31-4), and light calcium carbonate (CAS: 471-34-1) used in the following examples of the present invention were purchased from Chengdu Zero Six Biotechnology Co., Ltd.

[0025] Unless otherwise specified, the test materials used in the present invention are all common commercial products and can be purchased in the market.

[0026] In the raw material formula of the Ganoderma lucidum culture medium described in the present invention, corn stalks, peanut shells and Ganoderma lucidum residues are used as the base, wherein the Ganoderma lucidum residues refer to the residues remaining after the Ganoderma lucidum is extracted. The existing Ganoderma lucidum residues are discarded as waste, and the various Ganoderma lucidum active ingredients contained in the Ganoderma lucidum residues are far higher than any kind of wood; that is, the technical solution described in the present invention uses corn stalks, peanut shells and Ganoderma lucidum residues instead of wood as the base, which not only avoids the waste of tree resources, but also can make full use of crop shells, crop stalks and Ganoderma lucidum residues without discarding them, thereby avoiding adverse effects on the environment and further avoiding waste of resources.

[0027] Example 1

[0028] The culture medium for improving the survival rate of ganoderma lucidum comprises the following components in parts by weight: 70 parts of corn stalks, 12 parts of peanut shells, 13 parts of ganoderma lucidum residues, 1 part of gypsum powder, 2 parts of butyrylhydrazide, 1 part of phenylalanine and 2 parts of light calcium carbonate.

[0029] Preparation method:

[0030] Material crushing: crush corn stalks, peanut shells and ganoderma lucidum residue into 20 meshes respectively to obtain corn stalk powder, peanut shell powder and ganoderma lucidum residue powder;

[0031] Material mixing: Evenly mix corn straw powder, peanut shell powder, ganoderma lucidum residue powder, gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain culture medium. The water content of the culture medium is controlled at 60%.

[0032] Bag sterilization: Put the prepared culture medium into the edible fungus cultivation bag, seal it, put it into the high pressure sterilizer, sterilize it at 121℃ for 4h, and take it out after natural cooling for use.

[0033] Example 2

[0034] The culture medium for improving the survival rate of ganoderma lucidum comprises the following components in parts by weight: 65 parts of corn stalks, 15 parts of peanut shells, 15 parts of ganoderma lucidum residues, 2 parts of gypsum powder, 2 parts of butyrylhydrazide, 0.5 parts of phenylalanine and 1 part of light calcium carbonate.

[0035] Preparation method:

[0036] Material crushing: crush corn stalks, peanut shells and ganoderma lucidum residue into 20 meshes respectively to obtain corn stalk powder, peanut shell powder and ganoderma lucidum residue powder;

[0037] Material mixing: Evenly mix corn straw powder, peanut shell powder, ganoderma lucidum residue powder, gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain culture medium. The water content of the culture medium is controlled at 55%.

[0038] Bag sterilization: Put the prepared culture medium into the edible fungus cultivation bag, seal it, put it into the high pressure sterilizer, sterilize it at 121℃ for 4.5h, and take it out after natural cooling for use.

[0039] Example 3

[0040] The culture medium for improving the survival rate of ganoderma lucidum comprises the following components in parts by weight: 69 parts of corn stalks, 10 parts of peanut shells, 12 parts of ganoderma lucidum residues, 2 parts of gypsum powder, 2 parts of butyrylhydrazide, 1 part of phenylalanine and 2 parts of light calcium carbonate.

[0041] Preparation method:

[0042] Material crushing: crush corn stalks, peanut shells and ganoderma lucidum residue into 20 meshes respectively to obtain corn stalk powder, peanut shell powder and ganoderma lucidum residue powder;

[0043] Material mixing: Evenly mix corn straw powder, peanut shell powder, ganoderma lucidum residue powder, gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain culture medium. The water content of the culture medium is controlled at 65%.

[0044] Bag sterilization: Put the prepared culture medium into the edible fungus cultivation bag, seal it, put it into the high pressure sterilizer, sterilize it at 121℃ for 5h, and take it out after natural cooling for use.

[0045] Example 4

[0046] The culture medium for improving the survival rate of ganoderma lucidum comprises the following components in parts by weight: 67 parts of corn stalks, 14 parts of peanut shells, 10 parts of ganoderma lucidum residues, 2 parts of gypsum powder, 1 part of butyrylhydrazide, 0.5 part of phenylalanine and 1 part of light calcium carbonate.

[0047] Preparation method:

[0048] Material crushing: crush corn stalks, peanut shells and ganoderma lucidum residue into 20 meshes respectively to obtain corn stalk powder, peanut shell powder and ganoderma lucidum residue powder;

[0049] Material mixing: Evenly mix corn straw powder, peanut shell powder, ganoderma lucidum residue powder, gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain culture medium. The water content of the culture medium is controlled at 60%.

[0050] Bag sterilization: Put the prepared culture medium into the edible fungus cultivation bag, seal it, put it into the high pressure sterilizer, sterilize it at 121℃ for 4.5h, and take it out after natural cooling for use.

[0051] Comparative Example 1

[0052] The culture medium for improving the survival rate of ganoderma lucidum comprises the following components by weight: 70 parts of sawdust, 12 parts of peanut shells, 13 parts of ganoderma lucidum residues, 1 part of gypsum powder, 2 parts of butyrylhydrazide, 1 part of phenylalanine and 2 parts of light calcium carbonate (the only difference from Example 1 is that corn stalks are replaced with sawdust).

[0053] Preparation method:

[0054] Material crushing: crush sawdust, peanut shells and ganoderma lucidum residue into 20 meshes respectively to obtain sawdust powder, peanut shell powder and ganoderma lucidum residue powder;

[0055] Material mixing: Evenly mix sawdust powder, peanut shell powder, ganoderma lucidum residue powder, gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain culture medium. The water content of the culture medium is controlled at 60%.

[0056] Bag sterilization: Put the prepared culture medium into the edible fungus cultivation bag, seal it, put it into the high pressure sterilizer, sterilize it at 121℃ for 4h, and take it out after natural cooling for use.

[0057] Comparative Example 2

[0058] The culture medium for improving the survival rate of Ganoderma lucidum comprises the following components by weight: 70 parts of corn stalks, 12 parts of peanut shells, 13 parts of Ganoderma lucidum residues, 1 part of gypsum powder, 3 parts of NAA (naphthaleneacetic acid) and 2 parts of light calcium carbonate (the only difference from Example 1 is that butyrylhydrazide and phenylalanine are replaced by NAA).

[0059] Preparation method:

[0060] Material crushing: crush corn stalks, peanut shells and ganoderma lucidum residue into 20 meshes respectively to obtain corn stalk powder, peanut shell powder and ganoderma lucidum residue powder;

[0061] Material mixing: Evenly mix corn straw powder, peanut shell powder, ganoderma lucidum residue powder, gypsum powder, NAA and light calcium carbonate to obtain culture medium. The water content of the culture medium is controlled at 60%.

[0062] Bag sterilization: Put the prepared culture medium into the edible fungus cultivation bag, seal it, put it into the high pressure sterilizer, sterilize it at 121℃ for 4h, and take it out after natural cooling for use.

[0063] Comparative Example 3

[0064] The culture medium is prepared according to the culture medium formula selected from patent CN118511787A - a culture medium and a cultivation method for Ganoderma lucidum: 75% corn stalks, 22% sawdust, 1% lime powder, 1% gypsum and 1% ammonium sulfate (water content 65wt%).

[0065] Application Examples

[0066] Preparation of strains: Ganoderma lucidum mycelium is inoculated into PDA culture medium for activation, and cultured in a 25-28°C incubator until the mycelium fills the test tube.

[0067] Inoculation: 7 experimental groups were set up: Example 1-4 groups and Comparative Example 1-3 groups.

[0068] In the clean bench, the Ganoderma lucidum strains were rapidly inoculated into the sterilized cultivation bags of each group under aseptic conditions, sealed, and transferred to a dark culture room at 28°C and a relative humidity of about 60% for cultivation. The cultivation time was 60-65 days, and mushroom bags full of mycelium were obtained.

[0069] After-ripening of mycelium: Peel off the cultivation bag from the bag full of mycelium, place it in a greenhouse or under a forest with disinfected ground, cover the surface of the bag with a layer of plastic film, control the room temperature at 25-28℃, and maintain it for 5-7 days. During this period, lift the film twice a day for ventilation, and each time it is lifted for 10 minutes.

[0070] Covering with soil: After the after-ripening process, cover the surface of the mushroom bag with soil, with a thickness of 2-3cm. After covering with soil, irrigate it thoroughly, and then water it every 3-4 days to make the surface of the soil moist.

[0071] Management during the fruiting period of Ganoderma lucidum: The temperature of the mushroom room is 28-35℃, the air humidity is 80-90%, and before the mushroom buds of the mushroom bags covered with nutrient soil emerge, water them every 3-4 days to make the soil surface moist; when more than 50% of the mushroom bags have formed mushroom buds, water them thoroughly every 3 days until harvesting; stop spraying water for 5-7 days after each batch of harvesting to promote the recovery of mycelium growth. At the same time, observe and record the fruiting of Ganoderma lucidum in each group and calculate the survival rate.

[0072] Harvesting: When the light white or light yellow color on the edge of the Ganoderma lucidum cap disappears and the cap turns brown, it enters the spore powder ejection stage. After the spore powder ejection is completed, harvest the Ganoderma lucidum fruiting body, dry it at 60℃ and store it in a dry and ventilated place.

[0073] result

[0074] 1. Survival rate

[0075] Table 1 Survival rate results of each group

[0076]

[0077]

[0078] As can be seen from the results in Table 1, the survival rates of the groups of Examples 1-4 are all above 97%, among which the survival rate of Example 1 can reach 100%, and the number of seedlings is significantly more than that of the groups of Comparative Examples 1-3. Due to the change in components, the number of seedlings and the survival rate of Comparative Example 1-2 decreased significantly, and due to the different culture medium formula of Comparative Example 3 from that of Examples 1-4, the number of seedlings and the survival rate were also far less than those of Examples 1-4.

[0079] 2. Dry weight

[0080] The dry weight of individual Ganoderma lucidum cells in each group was counted and the average value was taken. The results are shown in Table 2.

[0081] Table 2 Dry weight of Ganoderma lucidum in each group

[0082] Group Dry weight / g Example 1 129.34 Example 2 130.41 Example 3 128.76 Example 4 129.03 Comparative Example 1 100.65 Comparative Example 2 109.62 Comparative Example 3 105.36

[0083] From the results in Table 2, it can be seen that while the number of seedlings and the survival rate were increased, the dry weight of the fungi in Example 1-4 groups did not decrease, and was still significantly higher than that in Comparative Example 1-3 groups.

[0084] In summary, the present invention provides a culture medium for improving the survival rate of ganoderma lucidum, which uses corn stalks, peanut shells and ganoderma lucidum residues instead of wood as a substrate, which not only avoids the waste of tree resources, but also makes full use of crop shells, crop stalks and ganoderma lucidum residues without discarding them, thereby avoiding adverse effects on the environment and further avoiding waste of resources. At the same time, adding danehydrazide and phenylalanine provides nutrition for ganoderma lucidum, promotes growth, and significantly improves the number of ganoderma lucidum seedlings, dry weight of bacteria and survival rate, and the survival rate can reach 100%.

[0085] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A culture medium for improving the survival rate of Ganoderma lucidum, characterized in that: The invention comprises the following components in parts by weight: 65-70 parts of corn stalks, 10-15 parts of peanut shells, 10-15 parts of ganoderma lucidum residues, 1-2 parts of gypsum powder, 1-2 parts of butyrylhydrazide, 0.5-1 parts of phenylalanine and 1-2 parts of light calcium carbonate.

2. The culture medium according to claim 1, characterized in that The water content of the culture medium is 55-65%.

3. The method for preparing the culture medium according to claim 1, characterized in that: The following steps are involved: The corn stalks, peanut shells and ganoderma lucidum residues are crushed, and then uniformly mixed with gypsum powder, butyrylhydrazide, phenylalanine and light calcium carbonate to obtain a culture medium; The culture medium is put into a bag and sterilized.

4. The preparation method according to claim 3, characterized in that: The corn stalks, peanut shells and ganoderma lucidum residues are crushed to 20 meshes.

5. The preparation method according to claim 3, characterized in that: The water content of the culture medium is 55-65%.

6. The preparation method according to claim 3, characterized in that: The sterilization condition is 121° C. for 4-5 hours.

7. Use of the culture medium according to any one of claims 1 to 2 in the cultivation of Ganoderma lucidum.

8. Use of the culture medium as claimed in any one of claims 1 to 2 in improving the survival rate of Ganoderma lucidum.

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