Solid beverage containing casein peptide and probiotics as well as preparation method and application of solid beverage

Abstract

The invention discloses a solid beverage containing casein peptide and probiotics and a preparation method of the solid beverage, and relates to the technical field of foods. The solid beverage comprises composite probiotic powder, casein peptide, gamma-aminobutyric acid, oligosaccharide, skim milk powder and fruit concentrated powder. The solid beverage not only effectively regulates intestinal flora, promotes food digestion, can obviously improve intestinal health, but also can further improve sleep quality.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and in particular relates to a solid beverage containing casein peptide and probiotics, and a preparation method and application thereof. Background Art

[0002] The overall sleep quality of Chinese residents is poor. On average, they fall asleep after midnight, and their sleep duration at night is generally short, with an average sleep duration of 6.75 hours and an average awakening frequency of 1.4 times. With the accelerated pace of life, rapid technological development, and increased work pressure, poor sleep quality and inability to fall asleep have become common problems that plague people.

[0003] There is a close relationship between sleep quality and intestinal flora. Intestinal flora refers to the microbial community in the human intestine, including bacteria, fungi, viruses, etc. They are closely related to our health and immune system. Studies have shown that an imbalance in the flora may lead to sleep problems, such as insomnia or sleep disorders. This is because the disorder of intestinal flora may cause inflammatory responses and immune system disorders, thereby affecting sleep quality. In addition, intestinal flora is also related to emotions and stress, which can also affect sleep. When we are under stress and anxiety, it may affect the balance of intestinal flora. And the disorder of flora may further affect sleep quality.

[0004] Due to the fast pace of modern society and changes in eating habits, the number of patients with intestinal dysfunction has gradually increased, which has adverse effects on the human body's internal environment and endocrine system, further inducing gastrointestinal-related diseases and even causing the appearance and aggravation of insomnia. While taking drugs to treat intestinal dysfunction to relieve gastrointestinal diseases, it may also cause side effects such as high blood pressure, severe emotional disorders, obvious myocardial pathological changes and increased mortality.

[0005] Casein peptides are a class of peptides with certain activity obtained by enzymatic hydrolysis, separation and purification of casein. Casein in cow's milk contains casein peptides that can promote sleep, also known as casein sleep peptides. Many studies have shown that casein peptides in milk have a significant effect on various aspects of people's sleep.

[0006] Probiotics are a type of active microorganisms that are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host. They promote nutrient absorption and maintain intestinal health by regulating the host mucosal and systemic immune functions or by regulating the balance of intestinal flora, thus producing single microorganisms or mixed microorganisms with clear composition that are beneficial to health. Summary of the invention

[0007] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a solid beverage containing casein peptides and probiotics that can effectively improve intestinal health and improve sleep quality.

[0008] To achieve the above object, the technical solution adopted by the present invention is: The invention provides a solid beverage containing casein peptide and probiotics. The solid beverage comprises: composite probiotic powder, casein peptide, gamma-aminobutyric acid, oligosaccharide, skim milk powder and fruit concentrated powder.

[0009] Preferably, the solid beverage containing casein peptides and probiotics comprises the following components by weight: 0.5-2.0 parts of composite probiotic powder, 1-3 parts of casein peptides, 5-20 parts of γ-aminobutyric acid, 30-60 parts of oligosaccharides, 5-10 parts of skim milk powder, and 8-18 parts of fruit concentrate powder.

[0010] Further preferably, the solid beverage containing casein peptides and probiotics comprises the following components in parts by weight: 1 part of compound probiotic powder, 3 parts of casein peptides, 20 parts of γ-aminobutyric acid, 51 parts of oligosaccharides, 10 parts of skim milk powder, and 15 parts of fruit concentrate powder.

[0011] The composite probiotic powder includes Lactobacillus rhamnosus LRa66, Lactobacillus fermentum LF61, Bifidobacterium animalis lactis subspecies BLa36, Lactobacillus paracasei LC37, and Lactobacillus plantarum N13; Furthermore, the viable count of Lactobacillus rhamnosus LRa66 is 600-800 billion CFU / g; Furthermore, the viable count of the fermented mucus Lactobacillus LF61 is 600-800 billion CFU / g; Furthermore, the viable count of the animal Bifidobacterium lactis subspecies BLa36 is 600-800 billion CFU / g; Furthermore, the viable count of Lactobacillus paracasei LC37 is 600-800 billion CFU / g; Furthermore, the viable bacterial count of Lactobacillus plantarum N13 is 600-800 billion CFU / g.

[0012] Furthermore, the mass ratio of Lactobacillus rhamnosus LRa66, Lactobacillus fermentative mucus LF61, Bifidobacterium animalis subspecies lactis BLa36, Lactobacillus paracasei LC37, and Lactobacillus plantarum N13 is (1-5): (1-5): (1-5): (1-5): 1.

[0013] Further preferably, the mass ratio of Lactobacillus rhamnosus LRa66, Lactobacillus fermentans LF61, Bifidobacterium animalis subspecies lactis BLa36, Lactobacillus paracasei LC37, and Lactobacillus plantarum N13 is 3:2:2:2:1.

[0014] The method for producing casein peptides comprises the following steps: Add water to casein at a mass ratio of 1:6-1:12 and stir, raise the temperature to 37-50°C, add protease with a casein mass of 0.05%-3.0% and digestive enzyme with a casein mass of 0.5%-4.0% in stages, perform two-step enzymolysis for a total of 3-8 hours, raise the temperature to inactivate the enzyme, and end the enzymolysis. The enzymolysis solution is filtered, concentrated and spray-dried to obtain casein peptides.

[0015] Furthermore, the source of the casein is cow's milk.

[0016] Furthermore, the protease includes one or more of papain, alkaline protease or chymotrypsin.

[0017] Furthermore, the digestive enzyme includes one or more of pepsin and pancreatin.

[0018] Preferably, the protease comprises papain or chymotrypsin, and the digestive enzyme is pepsin combined with pancreatic enzyme.

[0019] Furthermore, the mass of the protease added in the step is 0.1%-2.0% of the mass of the casein added.

[0020] Furthermore, the mass of the digestive enzyme added in the step is 0.5%-3.0% of the mass of the casein added.

[0021] Furthermore, the two-step enzymatic hydrolysis step specifically refers to: the first step of protease hydrolysis for 1-4 hours, and the second step of digestive enzyme hydrolysis for 2-4 hours.

[0022] Furthermore, the conditions for heating to inactivate the enzyme in the step are: heating to 100° C. and boiling for 15 minutes.

[0023] The gamma-aminobutyric acid is produced by using sodium L-glutamate as raw material through the steps of Lactobacillus shilgardii fermentation, heating sterilization, cooling, activated carbon treatment, filtering, adding blending auxiliary materials (starch), spray drying and the like.

[0024] The oligosaccharides include one or more of fructooligosaccharides, galacto-oligosaccharides, xylooligosaccharides and isomaltooligosaccharides.

[0025] More preferably, the oligosaccharide is a combination of fructo-oligosaccharide and galacto-oligosaccharide.

[0026] Furthermore, the mass ratio of the oligofructose to the oligogalactose is (1-3):1.

[0027] Furthermore, the mass ratio of the oligofructose to the oligogalactose is 3:1.

[0028] The fruit concentrated powder is one or more of blueberry concentrated powder, cranberry concentrated powder and passion fruit concentrated powder.

[0029] Further preferably, the fruit concentrate powder is obtained by spray-drying fruit concentrate by adding maltodextrin as a carrier.

[0030] More preferably, the proportion of fruit concentrated juice in the fruit concentrated powder is greater than 65%.

[0031] More preferably, the fruit concentrated powder is blueberry concentrated powder.

[0032] The present invention also provides a method for preparing the solid beverage containing casein peptides and probiotics, comprising the following steps: (1) Grinding the gamma-aminobutyric acid and mixing it with casein peptide, oligosaccharide, skim milk powder and fruit concentrate powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, and the drying is stopped. The composite probiotic powder is then added and mixed evenly to obtain a solid beverage containing casein peptides and probiotics.

[0033] The present invention also provides the use of the solid beverage containing casein peptides and probiotics in improving intestinal health and improving sleep quality.

[0034] Compared with the prior art, the beneficial effects of the present invention are: The present invention discloses a solid beverage containing casein peptides and probiotics. The casein peptides and probiotics interact with each other and synergize to enhance the effect, which can not only effectively regulate intestinal flora and promote food digestion, but also significantly improve intestinal health and further improve sleep quality. DETAILED DESCRIPTION

[0035] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Example 1

[0036] A solid beverage containing casein peptides and probiotics comprises the following components in parts by weight: 0.3 parts of Lactobacillus rhamnosus LRa66, 0.2 parts of Lactobacillus fermented mucus LF61, 0.2 parts of Bifidobacterium animalis lactis subsp. BLa36, 0.2 parts of Lactobacillus paracasei LC37, 0.2 parts of Lactobacillus plantarum N13, 3 parts of casein peptide, 20 parts of gamma-aminobutyric acid, 38.25 parts of fructooligosaccharides, 12.75 parts of galacto-oligosaccharides, 10 parts of skim milk powder, and 15 parts of blueberry concentrated powder.

[0037] The preparation method comprises the following steps: (1) Grinding the gamma-aminobutyric acid and mixing it with casein peptide, oligofructose, oligogalactose, skim milk powder, and blueberry concentrated powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, and the drying is stopped. The composite probiotic powder is then added and mixed evenly to obtain a solid beverage containing casein peptides and probiotics. Example 2

[0038] A solid beverage containing casein peptides and probiotics comprises the following components in parts by weight: 0.3 parts of Lactobacillus rhamnosus LRa66, 0.2 parts of Lactobacillus fermented mucus LF61, 0.2 parts of Bifidobacterium animalis lactis subsp. BLa36, 0.2 parts of Lactobacillus paracasei LC37, 0.2 parts of Lactobacillus plantarum N13, 2 parts of casein peptide, 20 parts of gamma-aminobutyric acid, 38.25 parts of fructooligosaccharides, 12.75 parts of galacto-oligosaccharides, 11 parts of skim milk powder, and 15 parts of blueberry concentrated powder.

[0039] The preparation method comprises the following steps: (1) Grinding the gamma-aminobutyric acid and mixing it with casein peptide, oligofructose, oligogalactose, skim milk powder, and blueberry concentrated powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, and the drying is stopped. The composite probiotic powder is then added and mixed evenly to obtain a solid beverage containing casein peptides and probiotics. Example 3

[0040] A solid beverage containing casein peptides and probiotics comprises the following components in parts by weight: 0.3 parts of Lactobacillus rhamnosus LRa66, 0.2 parts of Lactobacillus fermented mucus LF61, 0.2 parts of Bifidobacterium animalis lactis subsp. BLa36, 0.2 parts of Lactobacillus paracasei LC37, 0.2 parts of Lactobacillus plantarum N13, 1 part of casein peptide, 20 parts of gamma-aminobutyric acid, 38.25 parts of fructooligosaccharides, 12.75 parts of galacto-oligosaccharides, 12 parts of skim milk powder, and 15 parts of blueberry concentrated powder.

[0041] The preparation method comprises the following steps: (1) Grinding the gamma-aminobutyric acid and mixing it with casein peptide, oligofructose, oligogalactose, skim milk powder, and blueberry concentrated powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, and the drying is stopped. The composite probiotic powder is then added and mixed evenly to obtain a solid beverage containing casein peptides and probiotics.

[0042] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that no composite probiotic powder is added in Comparative Example 1, and the solid beverage of Comparative Example 1 comprises the following components in parts by weight: 3 parts of casein peptide, 20 parts of gamma-aminobutyric acid, 38.25 parts of fructooligosaccharides, 12.75 parts of galacto-oligosaccharides, 11 parts of skimmed milk powder, and 15 parts of blueberry concentrated powder.

[0043] The preparation method comprises the following steps: (1) Grinding the gamma-aminobutyric acid and mixing it with casein peptide, oligofructose, oligogalactose, skim milk powder, and blueberry concentrated powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, and the drying is stopped to obtain a solid beverage.

[0044] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that no casein peptide is added in Comparative Example 2, and the solid beverage of Comparative Example 2 comprises the following components in parts by weight: 0.3 parts of Lactobacillus rhamnosus LRa66, 0.2 parts of Lactobacillus fermentum LF61, 0.2 parts of Bifidobacterium animalis lactis subsp. BLa36, 0.2 parts of Lactobacillus paracasei LC37, 0.2 parts of Lactobacillus plantarum N13, 20 parts of gamma-aminobutyric acid, 38.25 parts of fructooligosaccharides, 12.75 parts of galacto-oligosaccharides, 13 parts of skim milk powder, and 15 parts of blueberry concentrated powder.

[0045] The preparation method comprises the following steps: (1) Grinding gamma-aminobutyric acid and mixing it with oligofructose, galacto-oligosaccharide, skim milk powder, and blueberry concentrated powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, the drying is stopped, and the composite probiotic powder is added and mixed evenly to obtain a solid beverage.

[0046] Test case: Sleep health and intestinal health test 150 people aged 25-45 with both sleep quality problems and intestinal problems were selected as volunteers. 120 volunteers were divided into 5 groups, and they tried the solid beverages of Examples 1-3 and Comparative Examples 1-2 respectively. Each person consumed 1 bag of solid beverage (specification 3g / bag) 30 minutes before going to bed every day for 1 month, and recorded and evaluated the sleep regulation and intestinal regulation, which were divided into no obvious improvement, improvement, and good improvement. During the test, the eating and resting habits of all volunteers remained consistent. The test results are shown in Table 1: Table 1 Sleep health and intestinal health test results

[0047] As can be seen from Table 1, the solid beverage casein peptide prepared by the present invention interacts with probiotics and synergizes to enhance the effect, which not only effectively regulates the intestinal flora, promotes the digestion of food, can significantly improve intestinal health, but also can further improve sleep quality.

[0048] It should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A solid beverage containing casein peptides and probiotics, characterized in that: The solid beverage comprises: compound probiotic powder, casein peptide, gamma-aminobutyric acid, oligosaccharide, skim milk powder and fruit concentrated powder.

2. The solid beverage containing casein peptide and probiotics according to claim 1, characterized in that: The solid beverage comprises the following components by weight: 0.5-2.0 parts of compound probiotic powder, 1-3 parts of casein peptide, 5-20 parts of gamma-aminobutyric acid, 30-60 parts of oligosaccharide, 5-10 parts of skim milk powder and 8-18 parts of fruit concentrate powder.

3. The solid beverage containing casein peptide and probiotics according to claim 2, characterized in that: The solid beverage containing casein peptide and probiotics comprises the following components by weight: 1 part of compound probiotic powder, 3 parts of casein peptide, 20 parts of gamma-aminobutyric acid, 51 parts of oligosaccharide, 10 parts of skim milk powder and 15 parts of fruit concentrate powder.

4. The solid beverage containing casein peptide and probiotics according to claim 3, characterized in that: The composite probiotic powder includes rhamnosus Lactobacillus LRa66, fermented mucus Lactobacillus LF61, animal bifidobacterium lactis subspecies BLa36, Lactobacillus paracasei LC37, and Lactobacillus plantarum N13. The number of live bacteria of rhamnosus Lactobacillus LRa66 is 600-800 billion CFU / g, the number of live bacteria of fermented mucus Lactobacillus LF61 is 600-800 billion CFU / g, the number of live bacteria of animal bifidobacterium lactis subspecies BLa36 is 600-800 billion CFU / g, the number of live bacteria of Lactobacillus paracasei LC37 is 600-800 billion CFU / g, and the number of live bacteria of Lactobacillus plantarum N13 is 600-800 billion CFU / g.

5. The solid beverage containing casein peptide and probiotics according to claim 4, characterized in that: The mass ratio of the Lactobacillus rhamnosus LRa66, the Lactobacillus fermentans LF61, the Bifidobacterium animalis lactis subspecies BLa36, the Lactobacillus paracasei LC37, and the Lactobacillus plantarum N13 is 3:2:2:2:

1.

6. The solid beverage containing casein peptides and probiotics according to claims 1-5, characterized in that: The oligosaccharides include one or more of fructooligosaccharides, galacto-oligosaccharides, xylooligosaccharides and isomaltooligosaccharides.

7. The solid beverage containing casein peptide and probiotics according to claim 6, characterized in that: The oligosaccharide is a combination of fructo-oligosaccharide and galacto-oligosaccharide, and the mass ratio of the fructo-oligosaccharide to the galacto-oligosaccharide is 3:

1.

8. The solid beverage containing casein peptide and probiotics and the preparation method thereof according to claims 1-7, characterized in that: The steps include: (1) Grinding the gamma-aminobutyric acid and mixing it with casein peptide, oligosaccharide, skim milk powder and fruit concentrate powder to obtain a mixed material; (2) The mixed material is placed in a boiling drying device for drying until the moisture content of the mixed material is less than 5%, and then the drying is stopped. The composite probiotic powder is then added and mixed evenly to obtain a solid beverage containing casein peptides and probiotics.

9. The solid beverage containing casein peptide and probiotics according to claim 8, characterized in that: Application in improving intestinal health and improving sleep quality.

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