Compound bacteria fermented compound traditional Chinese medicine scalp maintenance composition and application thereof

Abstract

The application discloses a compound bacteria fermentation compound traditional Chinese medicine scalp maintenance composition and application thereof, and belongs to the technical field of daily washing and protecting articles, and a preparation method thereof comprises the following steps: preparing a fermentation culture medium, preparing a lactobacillus liquid, preparing a yeast liquid, preparing a mixed bacteria liquid, fermenting, and mixing; the mixed material is mixed, homogenized and then the compound bacteria fermentation compound traditional Chinese medicine scalp maintenance composition is obtained, wherein the mixed material is the fermentation liquid, sodium hyaluronate, glycerol, phenoxyethanol, ethylhexylglycerin, butanediol, a stabilizer, dodecyl betaine and deionized water; the compound bacteria fermentation compound traditional Chinese medicine scalp maintenance composition prepared by the application has good storage resistance, the loss of important volatile substances can be reduced in the preparation, so that the natural active ingredients in traditional Chinese medicine can be maximized, further, the growth of scalp hair follicles can be promoted, scalp inflammation can be inhibited, hair glossiness can be increased, the permeability of active ingredients can be increased, and the effect of preventing hair loss can be improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hair care products, in particular to a compound bacteria fermented compound traditional Chinese medicine scalp care composition and application thereof. BACKGROUND

[0002] With the influence of the living environment, and the increase of life pressure, physical sub-health and other conditions, hair problems are also increasing, and problems such as dry and rough hair, excessive oil secretion on the scalp, dandruff, and sensitive scalp are prone to occur. The World Health Organization also lists "shiny hair and no dandruff" as one of the standards of human health. With consumers paying more and more attention to scalp care, various functional products are continuously developed and gradually refined. From the initial oil control, dandruff removal, care, and moisturizing functions, further extensions to hair blackening, hair loss prevention, and hair growth functions have been made.

[0003] For scalp and hair care products with repair function, the original hair care products mainly contain chemical synthetic substances, but long-term use can cause various side effects such as dry and yellowish hair, hair breakage, and hair loss. Therefore, more and more manufacturers have begun to produce plant care products by adding various herbal plants to solve the side effects caused by chemical synthetic substances. However, the care effect is not significant. Research has found that the plant ingredients in existing plant care products are mainly obtained by conventional pulverization of traditional Chinese medicinal materials, which contain a large amount of macromolecular or high polymer ingredients such as proteins, making it difficult to achieve good absorption and hindering the penetration of active substances. Today, some care products also use microbial fermentation of traditional Chinese medicine to increase the content of effective ingredients in traditional Chinese medicine. However, there are the following problems in the fermentation process of traditional Chinese medicine: first, the fermented traditional Chinese medicine is more susceptible to microbial contamination, resulting in poor storage resistance; second, traditional Chinese medicine contains volatile substances that are lost during fermentation, affecting the care effect. SUMMARY

[0004] In view of the deficiencies in the prior art, the present application provides a compound bacteria fermented compound traditional Chinese medicine scalp care composition and application thereof. The compound bacteria fermented compound traditional Chinese medicine scalp care composition of the present application has good storage resistance, and can reduce the loss of important volatile substances during preparation, thereby maximizing the effects of the rich natural active ingredients in traditional Chinese medicine. Furthermore, it can promote the growth of scalp hair follicles, inhibit scalp inflammation, increase hair gloss, increase the permeability of active ingredients, and also improve the effect of preventing hair loss.

[0005] To solve the above technical problems, the technical solutions adopted by the present application are as follows:

[0006] The application discloses a composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition and a preparation method thereof.

[0007] The preparation of the fermentation medium comprises the following steps: preparing a fermentation medium, preparing a lactobacillus liquid, preparing a yeast liquid, preparing a mixed bacteria liquid, fermenting, and mixing.

[0008] The mass ratio of the rehmannia glutinosa powder, the ligustri lucidi fructus powder, the polygonatum sibiricum powder, the biota orientalis powder and the chuanxiong rhizome powder is 20-30:10-25:10-20:20-40:8-20.

[0009] The mass-volume ratio of the mixed traditional Chinese medicine powder, the anhydrous ethanol and the deionized water is 100g:750-800mL:80-120mL.

[0010] The mass-volume ratio of the filtrate and the crosslinked microspheres is 800-850mL:7-8g.

[0011] The mass-volume ratio of the concentrated liquid and the glucose is 100mL:2.5-3.5g.

[0012] The preparation method of the crosslinked microspheres comprises the following steps: crosslinking and mixing.

[0013] The cross-linking, the sodium hyaluronate, the sodium hydroxide aqueous solution is mixed uniformly, sodium hyaluronate gel is obtained;The oil phase is stirred at the stirring speed of 400-500 rpm while the water phase is added dropwise into the oil phase, and then the high-pressure homogenizer is converted to homogenize, the pressure of the high-pressure homogenizer is controlled to be 120-150 MPa, and the homogenization times are 3-4 times, the homogenate is obtained;The homogenate is mixed with 1, 4-butanediol diglycidyl ether, and then stirred at the stirring speed of 300-500 rpm for 4-5 h, and then placed for 13-15 h, and then converted into a centrifuge, the rotation speed of the centrifuge is controlled to be 10000-11000 rpm, and the time is 9-10 min, and then the precipitate is taken, the precipitate is washed with ethyl acetate for 3-4 times, and then washed with anhydrous ethanol for 3-4 times, and then converted into an oven for drying, the temperature of the oven is controlled to be 60-70 DEG C, and the time is 14-16 h, and then sodium hyaluronate microspheres are obtained;

[0014] In the cross-linking, the molecular weight of the sodium hyaluronate is 1.5-2.5 million Da;

[0015] The sodium hyaluronate and the sodium hydroxide aqueous solution are in the mass-volume ratio of 10 g: 50-100 mL;

[0016] The mass concentration of the sodium hydroxide aqueous solution is 2%;

[0017] The mass-volume ratio of the Tween 80 and the liquid paraffin is 7 g: 370-400 mL;

[0018] The volume ratio of the oil phase and the water phase is 380-400: 510-560;

[0019] The dropwise time of the water phase is 40-50 min;

[0020] The volume ratio of the homogenate and 1, 4-butanediol diglycidyl ether is 900-950: 7-8;

[0021] The mixing, the kaolin and the sodium hyaluronate are added into the ball mill for ball milling, the ball-to-material ratio of the ball mill is controlled to be 10-12: 1, the rotation speed is 350-400 rpm, and the ball milling time is 1-1.5 h, and then the modified kaolin is obtained;The modified kaolin, the sodium hyaluronate microspheres and the deionized water are mixed, and then stirred at the stirring speed of 200-300 rpm for 2-3 h, and then converted into a centrifuge, the rotation speed of the centrifuge is controlled to be 8000-9000 rpm, and the time is 9-10 min, and then the precipitate is taken, washed with deionized water for 3-4 times, and then converted into an oven for drying, the temperature of the oven is controlled to be 60-80 DEG C, and the time is 28-30 h, and then the cross-linked microspheres are obtained;

[0022] The mass ratio of kaolin clay and sodium hyaluronate in the mixing is 30:10-12;

[0023] The mass-volume ratio of modified kaolin clay, sodium hyaluronate microspheres and deionized water is 20-22g:35-40g:900-1000mL;

[0024] The molecular weight of the sodium hyaluronate is 200,000 Da;

[0025] The preparation of the lactobacillus liquid, the lactobacillus is inoculated into the MRS culture medium for single strain enrichment culture, and is incubated at 35-37℃ for 40-48h to obtain a primary seed liquid, then the primary seed liquid is inoculated into the MRS culture medium at an inoculation amount of 2.5-3% (volume fraction), and is incubated at 35-37℃ for 20-25h to obtain a secondary seed liquid, i.e. the lactobacillus liquid;

[0026] The preparation method of the MRS culture medium is that the proteose peptone, beef extract, yeast extract, diammonium hydrogen citrate, glucose, sodium acetate, magnesium sulfate, manganese sulfate and distilled water are uniformly mixed, then the pH is adjusted to 6.2-6.6, and sterilized at 121℃ for 20-30min to obtain the MRS culture medium;

[0027] In the preparation of the MRS culture medium, the mass-volume ratio of proteose peptone, beef extract, yeast extract, diammonium hydrogen citrate, glucose, sodium acetate, magnesium sulfate, manganese sulfate and distilled water is 10-12g:5-6g:4-4.5g:2-2.1g:20-25g:5-6g:0.2-0.25g:0.05-0.06:100-130mL;

[0028] The bacterial content of the lactobacillus liquid is 1×10 9 -7×10 9 CFU / mL;

[0029] The preparation of the yeast liquid, the yeast is inoculated into the YPD culture medium for single strain enrichment culture, and is incubated at 28-30℃ for 20-24h to obtain a primary seed liquid, then the primary seed liquid is inoculated into the YPD culture medium at an inoculation amount of 2.5-3% (volume fraction), and is incubated at 28-30℃ for 35-36h to obtain a secondary seed liquid, i.e. the yeast liquid;

[0030] The preparation method of the YDP culture medium is that the yeast extract, proteose peptone, glucose and distilled water are uniformly mixed, the pH is adjusted to 6.2-6.6, and sterilized at 121℃ for 20-30min to obtain the YDP culture medium;

[0031] The mass-volume ratio of the yeast extract, the peptone, the glucose, and the distilled water in the YDP culture medium is 10-12g:20-22g:20-23g:100-120mL;

[0032] The bacterial content of the yeast bacterial liquid is 6×10 8 -1×10 9 CFU / mL;

[0033] The mixed bacterial liquid is prepared by mixing the lactobacillus bacterial liquid and the yeast bacterial liquid in a volume ratio of 1:1-1.5.

[0034] The fermentation liquid is obtained by inoculating the mixed bacterial liquid into the fermentation culture medium at an inoculation amount of 2-3% (volume fraction) and fermenting at 36-38℃ for 60-70h.

[0035] The compound bacterial fermentation compound traditional Chinese medicine scalp maintenance composition is obtained by mixing the fermentation liquid, the sodium hyaluronate, the glycerol, the phenoxyethanol, the ethylhexylglycerin, the butanediol, the stabilizer, the dodecyl betaine, and the deionized water and then transferring into a high-pressure homogenizer for homogenization, wherein the pressure of the high-pressure homogenizer is controlled to be 120-150MPa, and the homogenization times are 3-4 times.

[0036] In the mixing, the mass ratio of the fermentation liquid, the sodium hyaluronate, the glycerol, the phenoxyethanol, the ethylhexylglycerin, the butanediol, the stabilizer, the dodecyl betaine, and the deionized water is 20-30:1.5-2:3-5:0.5-1:0.01-1:4-5:3-3.5:1.5-2:75-80.

[0037] The molecular weight of the sodium hyaluronate is 100,000Da.

[0038] The stabilizer is prepared by adding the betaine, the acetylated sodium hyaluronate, and the kaolin into a ball mill for ball milling, wherein the ball-to-material ratio of the ball mill is controlled to be 10-12:1, the rotating speed is controlled to be 350-400rpm, and the ball milling time is controlled to be 2-2.5h.

[0039] In the preparation of the stabilizer, the mass ratio of the betaine, the acetylated sodium hyaluronate, and the kaolin is 20-25:12-15:90-100.

[0040] The acetyl substitution degree of the acetylated sodium hyaluronate is 3.

[0041] The application of the compound bacterial fermentation compound traditional Chinese medicine scalp maintenance composition.

[0042] Compared with the prior art, the application has the following beneficial effects:

[0043] (1) The composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition in the preparation, cross-linked microspheres are added in the preparation of fermentation medium step, specifically, cross-linked microspheres are added in the traditional Chinese medicine extract in the preparation of fermentation medium step, then concentrated, the cross-linked microspheres are prepared, first, sodium hyaluronate microspheres are prepared, then the modified kaolin is obtained by using small molecule sodium hyaluronate to intercalate and modify the surface of kaolin, then the modified kaolin is mixed with sodium hyaluronate microspheres, the modified kaolin is adsorbed in the cross-linked sodium hyaluronate microspheres by hydrogen bond to obtain cross-linked microspheres, so as to further increase the adsorption capacity of cross-linked sodium hyaluronate microspheres, then the cross-linked microspheres are added in the traditional Chinese medicine extract, the cross-linked microspheres can adsorb and release the volatile substances in the traditional Chinese medicine extract, so as to avoid the loss of volatile substances, improve the moisturizing effect, traditional Chinese medicine active substance content and hair follicle growth effect of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition, in addition, the cross-linked microspheres can also protect the volatile substances, so as to improve the storage resistance of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition; the stabilizer is added in the mixing, the preparation method of the stabilizer is that betaine, acetylated sodium hyaluronate and kaolin are mixed and ball milled, the betaine and acetylated sodium hyaluronate intercalate and modify the surface of kaolin during the ball milling process, the presence of betaine can promote the dispersion of kaolin and improve the antibacterial ability of kaolin, the molecular weight of acetylated sodium hyaluronate is smaller than that of sodium hyaluronate, and the water absorption is strong, after intercalating in kaolin, the moisture retention capacity of kaolin can be improved, the discomfort caused by the use of kaolin can be avoided, and the presence of kaolin can also stabilize, adsorb and release the active substances in traditional Chinese medicine, so as to improve the storage resistance of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition;

[0044] (2) Chinese medicine has a long history in preventing and treating hair loss, and has important significance in traditional medicine. Chinese medicine believes that the prevention and treatment of hair loss can be divided into five points, including: first, kidney deficiency: kidney is the main bone marrow, and its hair is in hair, so kidney deficiency can easily lead to hair loss; second, yin deficiency and fire: yin deficiency leads to fire, which can easily lead to blood heat and dryness, thereby consuming yin blood, so that the hair cannot be nourished, thereby causing hair loss; third, spleen deficiency: spleen deficiency can easily lead to the decline of the function of transporting and transforming water and nutrient essence, thereby leading to the lack of hair nourishment and causing hair loss; fourth, blood stasis: blood stasis can lead to blood stasis, and local blood circulation can easily cause hair loss; fifth, deficiency of qi and blood: hair is the remainder of blood, and if qi and blood are deficient, it can easily lead to dry and even falling hair. The composite bacteria fermented compound traditional Chinese medicine scalp care composition of the present application is mainly composed of tonifying, heat-clearing and blood-activating and stasis-removing drugs. The representative tonifying drug is rhizoma polygonati, radix rehmanniae preparata and ligustrum lucidum. The heat-clearing drug is biota leaf. The blood-activating and stasis-removing drug is chuanxiong. Among them, radix rehmanniae preparata is sweet and slightly warm, and belongs to liver and kidney channels, and contains benzene styrene glycoside such as verbascoside, monosaccharide, amino acid, vitamin A and other substances. Ligustrum lucidum is sweet and bitter, and belongs to liver and kidney channels, and contains triterpenoids, iridoid glycosides, flavonoids, fatty acids, volatile oil and polysaccharides. Biota leaf is bitter and slightly cold, and belongs to lung, liver, spleen and large intestine channels, and contains flavonoids such as quercetin, quercetin and kaempferol, volatile oil such as cypressene, and acetate pine oil, and tannins. Chuanxiong is pungent and warm, and belongs to liver, gallbladder and pericardium channels, and contains alkaloids, volatile oil, phenolic substances, endolipin, vitamin A, folic acid, sucrose, sterols and fatty oil. Rhizoma polygonati is sweet and flat, and belongs to spleen, lung and kidney channels, and contains polysaccharides, saponins and flavonoids.

[0045] (3) The composite bacteria fermented compound traditional Chinese medicine scalp care composition of the present application has no irritation to the skin, and no erythema and other allergic reactions are found on the skin surface after use in mice;

[0046] (4) The composite bacteria fermented compound traditional Chinese medicine scalp care composition of the present application can reduce the loss of important volatile substances in the preparation process, so that the rich natural active ingredients in traditional Chinese medicine can maximize the effect, further promote the growth of scalp hair follicles, inhibit scalp inflammation, increase hair gloss, increase the permeability of active ingredients, and improve the effect of preventing hair loss. The composite bacteria fermented compound traditional Chinese medicine scalp care composition of the present application can significantly improve the dry and rough hair, and the hair follicle hair growth rate can reach 180-220%;

[0047] (5) The composite bacteria fermented compound traditional Chinese medicine scalp care composition of the present application contains 0.64-0.70wt% of total flavonoids, 11.58-13.40wt% of total polyphenols and 10.36-12.42wt% of total sugar;

[0048] (6) The composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition of the present application has good storage resistance, after being placed in the open air at room temperature for 30 days, the total flavonoid content is 0.63-0.65 wt%, the total polyphenol content is 11.26-12.87 wt%, and the total sugar content is 10.12-12.14 wt%. DETAILED DESCRIPTION

[0049] In order to have a clearer understanding of the technical features, objects and effects of the present application, the specific embodiments of the present application are described below.

[0050] Example 1

[0051] A composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition, the preparation method thereof comprises the following steps:

[0052] 1. preparing a fermentation medium: selecting fresh, disease-free and pest-free rehmannia glutinosa, ligustrum lucidum, polygonatum, biota leaf and chuanqiong, cleaning them respectively, and then crushing them to 120 mesh to obtain rehmannia glutinosa powder, ligustrum lucidum powder, polygonatum powder, biota leaf powder and chuanqiong powder, then mixing the rehmannia glutinosa powder, ligustrum lucidum powder, polygonatum powder, biota leaf powder and chuanqiong powder uniformly according to a mass ratio of 20:10:10:20:8 to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, anhydrous ethanol and deionized water according to a mass-volume ratio of 100g:750mL:80mL, then soaking at room temperature for 3h, performing ultrasonic reflux extraction, controlling the ultrasonic reflux extraction temperature to be 65℃, the ultrasonic power to be 90W, and the time to be 1h, then filtering, taking the filtrate, adding cross-linked microspheres to the filtrate, controlling the mass-volume ratio of the filtrate and the cross-linked microspheres to be 800mL:7g, obtaining a mixed solution, then concentrating the mixed solution to 20% of the original volume by a rotary evaporator, then adjusting the pH to 6 to obtain a concentrated solution, mixing the concentrated solution and glucose according to a mass-volume ratio of 100mL:2.5g, sterilizing at 121℃ for 20min, naturally cooling to room temperature, and obtaining the fermentation medium;

[0053] The preparation method of the cross-linked microspheres is as follows:

[0054] (1) Crosslinking: mix sodium hyaluronate and a 2% sodium hydroxide aqueous solution in a mass-volume ratio of 10 g:50 mL to obtain a sodium hyaluronate gel; mix Tween 80 and liquid paraffin in a mass-volume ratio of 7 g:370 mL to obtain an oil phase; while stirring the oil phase at a stirring speed of 400 rpm, add the water phase to the oil phase, control the volume ratio of the oil phase to the water phase to be 380:510, and the dropwise addition time of the water phase is 40 min; after the dropwise addition is completed, transfer into a high-pressure homogenizer for homogenization, control the pressure of the high-pressure homogenizer to be 120 MPa, and the homogenization times is 3 times, to obtain a homogenized liquid; mix the homogenized liquid and 1,4-butanediol diglycidyl ether in a volume ratio of 900:7, stir at a stirring speed of 300 rpm for 4 h, stand for 13 h, transfer into a centrifuge, control the rotation speed of the centrifuge to be 10000 rpm, and the time is 9 min, take the precipitate, wash the precipitate with ethyl acetate for 3 times, wash the precipitate with anhydrous ethanol for 3 times, transfer into an oven for drying, control the temperature of the oven to be 60℃, and the time is 14 h, to obtain sodium hyaluronate microspheres;

[0055] The molecular weight of the sodium hyaluronate is 1.5 million Da;

[0056] (2) Mixing: add kaolin and sodium hyaluronate into a ball mill in a mass ratio of 30:10 for ball milling, control the ball-to-material ratio of the ball mill to be 10:1, and the rotation speed is 350 rpm, and ball mill for 1 h to obtain modified kaolin; mix the modified kaolin, sodium hyaluronate microspheres, and deionized water in a mass-volume ratio of 20 g:35 g:900 mL, stir at a stirring speed of 200 rpm for 2 h, then transfer into a centrifuge, control the rotation speed of the centrifuge to be 8000 rpm, and the time is 9 min, take the precipitate, wash with deionized water for 3 times, transfer into an oven for drying, control the temperature of the oven to be 70℃, and the time is 28 h, to obtain crosslinked microspheres;

[0057] The molecular weight of the sodium hyaluronate is 200 thousand Da;

[0058] 2. Preparation of lactobacillus bacterial liquid: inoculate lactobacillus into MRS culture medium for single-strain strain enrichment culture, stand for culture at 35℃ for 40 h to obtain a primary seed liquid, then inoculate the primary seed liquid into MRS culture medium at an inoculation amount of 2.5% (volume fraction), stand for culture at 35℃ for 20 h to obtain a secondary seed liquid, i.e. lactobacillus bacterial liquid;

[0059] The preparation method of the MRS culture medium is as follows: 10 g of peptone, 5 g of beef extract, 4 g of yeast extract, 2 g of diammonium hydrogen citrate, 20 g of glucose, 5 g of sodium acetate, 0.2 g of magnesium sulfate, 0.05-0.06 g of manganese sulfate, and 100 mL of distilled water are uniformly mixed, then the pH is adjusted to 6.2, sterilization is carried out at 121 DEG C for 30 min, and the MRS culture medium is obtained;

[0060] The bacterial content of the lactobacillus bacterial solution is 1x10 9 CFU / mL;

[0061] 3. The yeast bacterial solution is prepared as follows: the yeast bacteria are inoculated into the YPD culture medium for single-strain strain enrichment culture, and static culture is carried out at 28 DEG C for 20 h to obtain a primary seed solution; then the primary seed solution is inoculated into the YPD culture medium at an inoculation amount of 2.5% (volume fraction), and static culture is carried out at 28 DEG C for 35 h to obtain a secondary seed solution, i.e. the yeast bacterial solution;

[0062] The preparation method of the YDP culture medium is as follows: 10 g of yeast extract, 20 g of peptone, 20 g of glucose, and 100 mL of distilled water are uniformly mixed, the pH is adjusted to 6.2, sterilization is carried out at 121 DEG C for 30 min, and the YDP culture medium is obtained;

[0063] The bacterial content of the yeast bacterial solution is 6x10 8 CFU / mL;

[0064] 4. The mixed bacterial solution is prepared as follows: the lactobacillus bacterial solution and the yeast bacterial solution are uniformly mixed at a volume ratio of 1:1 to obtain the mixed bacterial solution;

[0065] 5. Fermentation: the mixed bacterial solution is inoculated into the fermentation culture medium at an inoculation amount of 2% (volume fraction), and fermentation is carried out at 36 DEG C for 60 h to obtain a fermentation liquor;

[0066] 6. Mixing: the fermentation liquor, sodium hyaluronate, glycerol, phenoxyethanol, ethylhexylglycerin, butanediol, a stabilizer, dodecyl betaine, and deionized water are mixed at a mass ratio of 20:1.5:3:0.5:0.01:4:3:1.5:75, and then transferred into a high-pressure homogenizer for homogenization, wherein the pressure of the high-pressure homogenizer is controlled at 120 MPa, and the homogenization frequency is 3 times, to obtain a composite bacterial fermentation compound traditional Chinese medicine scalp maintenance composition;

[0067] The molecular weight of the sodium hyaluronate is 100,000 Da;

[0068] The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate, and kaolin are added into a ball mill for ball milling, the ball-to-material ratio of the ball mill is controlled at 10:1, the rotating speed is controlled at 350 rpm, and the ball milling time is 2 h, to obtain the stabilizer;

[0069] The acetyl degree of the acetylated sodium hyaluronate is 3.

[0070] The present embodiment also provides an application of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition.

[0071] Example 2

[0072] A composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition, a preparation method thereof comprises the following steps:

[0073] 1. Preparation of fermentation medium: select fresh, no disease and insect pests of rehmannia glutinosa, ligustrum lucidum, polygonatum, biota leaf, chuanqiong, respectively clean after crushing to 120 mesh, get rehmannia glutinosa powder, ligustrum lucidum powder, polygonatum powder, biota leaf powder, chuanqiong powder, then mix rehmannia glutinosa powder, ligustrum lucidum powder, polygonatum powder, biota leaf powder, chuanqiong powder according to the quality ratio of 25:20:15:30:15, get mixed traditional Chinese medicine powder, mix mixed traditional Chinese medicine powder, anhydrous ethanol, deionized water according to the quality volume ratio of 100g:780ml:100ml, soak for 3.5h at room temperature, ultrasonic reflux extraction, control the temperature of ultrasonic reflux extraction is 68℃, ultrasonic power is 100w, time is 1.5h, then filter, take the filtrate, add crosslinked microspheres to the filtrate, control the quality volume ratio of the filtrate and crosslinked microspheres is 820ml:7.5g, get mixed liquid, then concentrate the mixed liquid to 23% of the original volume by rotary evaporator, then adjust the pH to 6.5, get concentrated solution, mix the concentrated solution and glucose according to the quality volume ratio of 100ml:3g, mix evenly, sterilize at 121℃ for 25min, cool to room temperature naturally, get fermentation medium;

[0074] The preparation method of the crosslinked microspheres is:

[0075] (1) Crosslinking: mix sodium hyaluronate and a 2% sodium hydroxide aqueous solution in a mass-volume ratio of 10 g:80 mL to obtain a sodium hyaluronate gel; mix Tween 80 and liquid paraffin in a mass-volume ratio of 7 g:380 mL to obtain an oil phase; while stirring the oil phase at a stirring speed of 450 rpm, add the water phase to the oil phase, control the volume ratio of the oil phase to the water phase to be 390:550, and control the dropwise addition time of the water phase to be 45 min; after the dropwise addition is completed, transfer the mixture into a high-pressure homogenizer to homogenize, control the pressure of the high-pressure homogenizer to be 140 MPa, and control the homogenization times to be 3 times to obtain a homogenized liquid; mix the homogenized liquid and 1,4-butanediol diglycidyl ether in a volume ratio of 920:7.5, stir at a stirring speed of 400 rpm for 4.5 h, stand for 14 h, transfer into a centrifuge, control the rotation speed of the centrifuge to be 10,000 rpm, and control the time to be 10 min, take the precipitate, wash the precipitate with ethyl acetate for 3 times, wash the precipitate with anhydrous ethanol for 3 times, transfer into an oven to dry, control the temperature of the oven to be 65 °C, and control the time to be 15 h to obtain sodium hyaluronate microspheres;

[0076] The molecular weight of the sodium hyaluronate is 2 million Da;

[0077] (2) Mixing: put kaolin and sodium hyaluronate into a ball mill in a mass ratio of 30:11 to perform ball milling, control the ball-to-material ratio of the ball mill to be 11:1, control the rotation speed to be 380 rpm, and control the ball milling time to be 1 h to obtain modified kaolin; mix the modified kaolin, the sodium hyaluronate microspheres, and deionized water in a mass-volume ratio of 21 g:38 g:950 mL, stir at a stirring speed of 200 rpm for 2.5 h, then transfer into a centrifuge, control the rotation speed of the centrifuge to be 8,500 rpm, control the time to be 10 min, take the precipitate, wash the precipitate with deionized water for 3 times, transfer into an oven to dry, control the temperature of the oven to be 75 °C, and control the time to be 29 h to obtain crosslinked microspheres;

[0078] The molecular weight of the sodium hyaluronate is 200,000 Da;

[0079] 2. Prepare a lactobacillus bacterial solution: inoculate lactobacillus into MRS culture medium to perform single-strain strain enrichment culture, stand for culture at 36 °C for 45 h to obtain a primary seed solution, then inoculate the primary seed solution into MRS culture medium in an inoculation amount of 2.5% (volume fraction), stand for culture at 36 °C for 22 h to obtain a secondary seed solution, i.e., a lactobacillus bacterial solution;

[0080] The preparation method of the MRS culture medium is as follows: 11 g of peptone, 5.5 g of beef extract, 4.2 g of yeast extract, 2 g of diammonium hydrogen citrate, 22 g of glucose, 5.5 g of sodium acetate, 0.22 g of magnesium sulfate, 0.05 g of manganese sulfate, and 120 mL of distilled water are uniformly mixed, then the pH is adjusted to 6.5, and sterilization is performed at 121 DEG C for 30 min to obtain the MRS culture medium;

[0081] The bacterial content of the lactobacillus bacterial solution is 5x10 9 CFU / mL;

[0082] 3. The yeast bacterial solution is prepared as follows: the yeast bacteria are inoculated into the YPD culture medium for single-strain strain enrichment culture, and static culture is performed at 29 DEG C for 22 h to obtain a primary seed solution, then the primary seed solution is inoculated into the YPD culture medium at an inoculation amount of 2.8% (volume fraction), and static culture is performed at 29 DEG C for 35.5 h to obtain a secondary seed solution, i.e., the yeast bacterial solution;

[0083] The preparation method of the YDP culture medium is as follows: 11 g of yeast extract, 21 g of peptone, 22 g of glucose, and 110 mL of distilled water are uniformly mixed, the pH is adjusted to 6.5, and sterilization is performed at 121 DEG C for 30 min to obtain the YDP culture medium;

[0084] The bacterial content of the yeast bacterial solution is 9x10 8 CFU / mL;

[0085] 4. The mixed bacterial solution is prepared as follows: the lactobacillus bacterial solution and the yeast bacterial solution are uniformly mixed at a volume ratio of 1:1.2 to obtain the mixed bacterial solution;

[0086] 5. Fermentation: the mixed bacterial solution is inoculated into the fermentation culture medium at an inoculation amount of 2.5% (volume fraction), and fermentation is performed at 37 DEG C for 65 h to obtain a fermentation liquor;

[0087] 6. Mixing: the fermentation liquor, sodium hyaluronate, glycerol, phenoxyethanol, ethylhexylglycerin, butanediol, a stabilizer, dodecyl betaine, and deionized water are mixed at a mass ratio of 25:1.8:4:0.8:0.4:4.5:3.2:1.8:78, and then transferred into a high-pressure homogenizer for homogenization, wherein the pressure of the high-pressure homogenizer is controlled to be 140 MPa, and the homogenization frequency is 3 times to obtain the composite bacterial fermentation compound traditional Chinese medicine scalp maintenance composition;

[0088] The molecular weight of the sodium hyaluronate is 100,000 Da;

[0089] The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate, and kaolin are added into a ball mill for ball milling, the ball-to-material ratio of the ball mill is controlled to be 11:1, the rotating speed is controlled to be 380 rpm, and the ball milling time is 2 h to obtain the stabilizer.

[0090] The acetyl degree of the acetylated sodium hyaluronate is 3.

[0091] The present embodiment also provides an application of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition.

[0092] Example 3

[0093] A composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition, a preparation method thereof comprises:

[0094] 1. Preparation of fermentation medium: select fresh, no disease and insect pests of rehmannia glutinosa, ligustrum lucidum, polygonatum, biota leaf, chuanqiong, respectively clean after crushing to 150 mesh, get rehmannia glutinosa powder, ligustrum lucidum powder, polygonatum powder, biota leaf powder, chuanqiong powder, then mix rehmannia glutinosa powder, ligustrum lucidum powder, polygonatum powder, biota leaf powder, chuanqiong powder according to the quality ratio of 30:25:20:40:20, get mixed traditional Chinese medicine powder, mix mixed traditional Chinese medicine powder, anhydrous ethanol, deionized water according to the quality volume ratio of 100g:800mL:120mL, soak for 4h at room temperature, ultrasonic reflux extraction, control the temperature of ultrasonic reflux extraction is 70℃, ultrasonic power is 120w, time is 1.5h, then filter, take the filtrate, add crosslinked microspheres to the filtrate, control the quality volume ratio of the filtrate and crosslinked microspheres is 850mL:8g, get mixed liquid, then concentrate the mixed liquid to 25% of the original volume by rotary evaporator, then adjust the pH to 7, get concentrated solution, mix the concentrated solution and glucose according to the quality volume ratio of 100mL:3.5g, mix evenly, sterilize at 100℃ for 30min, cool to room temperature naturally, get fermentation medium;

[0095] The preparation method of the crosslinked microspheres is:

[0096] (1) Crosslinking: mix sodium hyaluronate and a 2% sodium hydroxide aqueous solution in a mass-volume ratio of 10 g: 100 mL to obtain a sodium hyaluronate gel; mix Tween 80 and liquid paraffin in a mass-volume ratio of 7 g: 400 mL to obtain an oil phase; while stirring the oil phase at a stirring speed of 500 rpm, add the water phase to the oil phase, control the volume ratio of the oil phase to the water phase to be 400: 560, and the dropwise addition time of the water phase is 50 min; after the dropwise addition is completed, transfer into a high-pressure homogenizer for homogenization, control the pressure of the high-pressure homogenizer to be 150 MPa, and the homogenization times is 4 times to obtain a homogenized liquid; mix the homogenized liquid with 1,4-butanediol diglycidyl ether in a volume ratio of 950: 8, stir at a stirring speed of 500 rpm for 5 h, stand for 15 h, transfer into a centrifuge, control the rotation speed of the centrifuge to be 11000 rpm, and the time is 10 min, take the precipitate, wash the precipitate with ethyl acetate 4 times, wash the precipitate with anhydrous ethanol 4 times, transfer into an oven for drying, control the temperature of the oven to be 70℃, and the time is 16 h to obtain sodium hyaluronate microspheres;

[0097] The molecular weight of the sodium hyaluronate is 2 million Da;

[0098] (2) Mixing: add kaolin and sodium hyaluronate into a ball mill in a mass ratio of 30: 12 for ball milling, control the ball-to-material ratio of the ball mill to be 12: 1, and the rotation speed is 400 rpm, ball mill for 1.5 h to obtain modified kaolin; mix the modified kaolin, sodium hyaluronate microspheres, and deionized water in a mass-volume ratio of 22 g: 40 g: 1000 mL, stir at a stirring speed of 300 rpm for 3 h, then transfer into a centrifuge, control the rotation speed of the centrifuge to be 9000 rpm, and the time is 10 min, take the precipitate, wash 4 times with deionized water, transfer into an oven for drying, control the temperature of the oven to be 80℃, and the time is 30 h to obtain crosslinked microspheres;

[0099] The molecular weight of the sodium hyaluronate is 200 thousand Da;

[0100] 2. Preparation of lactobacillus bacterial liquid: inoculate lactobacillus into MRS culture medium for single strain enrichment culture, stand for culture at 37℃ for 48 h to obtain a primary seed liquid, then inoculate the primary seed liquid into MRS culture medium at an inoculation amount of 3% (volume fraction), stand for culture at 37℃ for 25 h to obtain a secondary seed liquid, i.e. lactobacillus bacterial liquid;

[0101] The preparation method of the MRS culture medium is as follows: 12 g of peptone, 6 g of beef extract, 4.5 g of yeast extract, 2.1 g of diammonium hydrogen citrate, 25 g of glucose, 6 g of sodium acetate, 0.25 g of magnesium sulfate, 0.06 g of manganese sulfate, and 130 mL of distilled water are uniformly mixed, then the pH is adjusted to 6.6, sterilization is performed at 121 DEG C for 30 min, and the MRS culture medium is obtained;

[0102] The bacterial content of the lactobacillus bacterial solution is 7 x 10 9 CFU / mL;

[0103] 3. The yeast bacterial solution is prepared as follows: the yeast bacteria are inoculated into YPD culture medium for single-strain strain enrichment culture, and static culture is performed at 30 DEG C for 24 h to obtain a primary seed solution; then the primary seed solution is inoculated into YPD culture medium at a inoculation amount of 3% (volume fraction), and static culture is performed at 30 DEG C for 36 h to obtain a secondary seed solution, i.e., the yeast bacterial solution;

[0104] The preparation method of the YDP culture medium is as follows: 12 g of yeast extract, 22 g of peptone, 23 g of glucose, and 120 mL of distilled water are uniformly mixed, the pH is adjusted to 6.6, sterilization is performed at 121 DEG C for 30 min, and the YDP culture medium is obtained;

[0105] The bacterial content of the yeast bacterial solution is 1 x 10 9 CFU / mL;

[0106] 4. The mixed bacterial solution is prepared as follows: the lactobacillus bacterial solution and the yeast bacterial solution are uniformly mixed at a volume ratio of 1:1.5 to obtain the mixed bacterial solution;

[0107] 5. Fermentation: the mixed bacterial solution is inoculated into fermentation culture medium at a inoculation amount of 3% (volume fraction), and fermentation is performed at 38 DEG C for 70 h to obtain a fermentation liquor;

[0108] 6. Mixing: the fermentation liquor, sodium hyaluronate, glycerol, phenoxyethanol, ethylhexylglycerin, butanediol, a stabilizer, dodecyl betaine, and deionized water are mixed at a mass ratio of 30:2:5:1:1:5:3.5:2:80, and then transferred into a high-pressure homogenizer for homogenization, the pressure of the high-pressure homogenizer is controlled to be 150 MPa, and the homogenization frequency is 4 times to obtain the compound bacterial fermentation compound traditional Chinese medicine scalp maintenance composition;

[0109] The molecular weight of the sodium hyaluronate is 100,000 Da;

[0110] The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate, and kaolin are added into a ball mill for ball milling, the ball-to-material ratio of the ball mill is controlled to be 12:1, the rotating speed is controlled to be 400 rpm, and the ball milling time is 2.5 h to obtain the stabilizer;

[0111] The acetyl substitution degree of the acetylated sodium hyaluronate is 3.

[0112] The present embodiment also provides an application of the aforementioned composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition.

[0113] Comparative Example 1

[0114] The present comparative example is based on the preparation method of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition of Example 2, and in the step of preparing the fermentation medium in the first step, the cross-linked microspheres are omitted; the rest of the operations are the same as those of Example 2.

[0115] The present comparative example also provides an application of the aforementioned composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition.

[0116] Comparative Example 2

[0117] The present comparative example is based on the preparation method of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition of Example 2, and in the step of mixing in the sixth step, the stabilizer is omitted; the rest of the operations are the same as those of Example 2.

[0118] The present comparative example also provides an application of the aforementioned composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition.

[0119] Test Example 1: Skin irritation test

[0120] 60 mice with a body weight of 20-25 g were selected and evenly divided into 6 groups, 10 mice in each group. The back hair of the mice was scraped clean, and the composite bacteria fermented compound traditional Chinese medicine scalp maintenance compositions prepared in Examples 1-3 and Comparative Examples 1-2 were applied to the backs of the mice in groups 1-5, respectively. The mice in group 6 were used as a blank control and were not applied. After 4 hours, the backs of the mice in groups 1-5 were washed clean, the skin conditions at the applied sites were observed, and the results were recorded as follows:

[0121]

[0122] Test Example 2: Moisturizing test

[0123] 75 adult women with dry and itchy hair between the ages of 25-45 were selected and divided into 5 groups, 15 women in each group. The composite bacteria fermented compound traditional Chinese medicine scalp maintenance compositions of Examples 1-3 and Comparative Examples 1-2 were applied to the women in groups 1-5, respectively, once a day, and the test lasted for 30 days.

[0124] In the test, the shampooing time and frequency of all the volunteers were kept consistent, and after shampooing, the compound traditional Chinese medicine scalp maintenance composition serum was applied to the scalp and hair and massaged for 2 minutes.

[0125] After the test, the improvement degree of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition on the dry and rough hair was investigated by questionnaire, and the investigation results were as follows:

[0126]

[0127] Test example 3: Detection of active ingredients of traditional Chinese medicine

[0128] The total flavonoids, total polyphenols and total sugar contents in the composite bacteria fermented compound traditional Chinese medicine scalp maintenance compositions of examples 1-3 and comparative examples 1-2 were detected, and the detection methods and detection results were as follows:

[0129] The detection method of the active ingredients of traditional Chinese medicine-total flavonoids: according to the first part of Chinese Pharmacopoeia P30, the total flavonoids were detected by referring to the total flavonoids detection method in hawthorn leaves of Pharmacopoeia;

[0130] The detection method of the active ingredients of traditional Chinese medicine-total polyphenols: according to GB / T 8313-2008 (second method) for detection;

[0131] The detection method of the active ingredients of traditional Chinese medicine-total sugar content: the total sugar content in the sample was detected by the sulfanilic acid method.

[0132]

[0133] Test example 4: hair follicle growth test

[0134] The hair follicle growth effect of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance compositions of examples 1-3 and comparative examples 1-2 was detected, and the detection methods and detection results were as follows:

[0135] The hair follicles of adult humans with hair loss were selected for the test, and the intact and undamaged hair follicles in the growth period were selected under a dissecting microscope. The selected free hair follicles were carefully moved into a 24-well culture plate, one hair follicle per well, 0.5 mL of William's E serum-free medium was added, and the suspension culture was carried out in a constant temperature incubator at 37°C and 5% CO2. After 24 h, 60 hair follicles with obvious growth (>0.1 mm) were selected, and the length of each hair follicle was measured as the initial hair follicle length. Then, the 60 hair follicles were evenly divided into 6 groups, 10 in each group, and labeled as groups 1-6. The composite bacteria fermented compound traditional Chinese medicine scalp maintenance compositions of examples 1-3 and comparative examples 1-2 were added to groups 1-5 for continuous culture without medium replacement, and group 6 was directly cultured without the addition of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition without medium replacement.

[0136] After 15 days of continuous culture, the length of each hair follicle was measured as the final hair follicle length, and then the hair follicle growth rate was calculated. The calculation formula of the hair follicle growth rate was as follows:

[0137] Hair follicle hair growth rate = (final hair follicle length - initial hair follicle length) / initial hair follicle length * 100%;

[0138] Then the average value of the hair follicle hair growth rate of each group is calculated, and the calculation results are as follows:

[0139]

[0140] Storage resistance test

[0141] After the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition of examples 1-3 and comparative examples 1-2 is placed open at room temperature for 30 days, the important active content is detected according to the method of test example 3, and the detection results are as follows:

[0142]

[0143] From the results of test example 1-5, it can be seen that by adding cross-linked microspheres in the preparation of the fermentation medium, the moisturizing effect, the active content of traditional Chinese medicine, the hair follicle growth effect and the storage resistance of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition prepared can be improved.

[0144] By adding a stabilizer in the mixture, the moisturizing effect and storage resistance of the composite bacteria fermented compound traditional Chinese medicine scalp maintenance composition prepared can be improved.

[0145] Unless otherwise specified, the percentages used in the present application are mass percentages.

[0146] Finally, it should be noted that: the above only describes the preferred embodiments of the present application and is not used to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, the technical solutions recorded in the foregoing embodiments can still be modified, or some technical features can be replaced equivalently. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A compound herbal scalp care composition fermented with compound bacteria, characterized in that, Its preparation method consists of the following steps: preparing fermentation culture medium, preparing lactobacillus bacterial solution, preparing yeast bacterial solution, preparing mixed bacterial solution, fermentation, and mixing. The fermentation medium is prepared by selecting fresh, disease-free Rehmannia glutinosa, Ligustrum lucidum, Polygonatum sibiricum, Platycladus orientalis leaves, and Ligusticum chuanxiong. These herbs are cleaned and pulverized to obtain Rehmannia glutinosa powder, Ligustrum lucidum powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Ligusticum chuanxiong powder. These powders are then mixed evenly to obtain a mixed herbal powder. The mixed herbal powder is then mixed with anhydrous ethanol and deionized water, soaked at room temperature, and subjected to ultrasonic reflux extraction. The mixture is then filtered, and the filtrate is collected. Cross-linked microspheres are added to the filtrate to obtain a mixed solution. This solution is then concentrated to 20-25% of its original volume using a rotary evaporator. The pH is adjusted to 6-7 to obtain a concentrated solution. This concentrated solution is then mixed evenly with glucose, sterilized, and naturally cooled to room temperature to obtain the fermentation medium. In the preparation of the fermentation medium, the mass ratio of Rehmannia glutinosa powder, Ligustrum lucidum powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Ligusticum chuanxiong powder is 20-30:10-25:10-20:20-40:8-20; The mass-to-volume ratio of the mixed Chinese herbal powder, anhydrous ethanol, and deionized water is 100g:750-800mL:80-120mL; The mass-to-volume ratio of filtrate to cross-linked microspheres is 800-850 mL: 7-8 g; The concentrate and glucose were mixed at a mass-to-volume ratio of 100 mL: 2.5-3.5 g. The ultrasonic reflux extraction temperature is 65-70℃, the ultrasonic power is 90-120W, and the time is 1-1.5h. The method for preparing the cross-linked microspheres comprises the following steps: cross-linking and mixing; The crosslinking process involves uniformly mixing sodium hyaluronate and sodium hydroxide aqueous solution to obtain sodium hyaluronate gel; uniformly mixing Tween 80 and liquid paraffin to obtain an oil phase; while stirring the oil phase, adding the aqueous phase dropwise to the oil phase; after the addition is complete, transferring the mixture to a high-pressure homogenizer for homogenization to obtain a homogenized liquid; mixing the homogenized liquid with 1,4-butanediol diglycidyl ether, stirring, allowing it to stand, centrifuging, collecting the precipitate, washing and drying the precipitate to obtain sodium hyaluronate microspheres. In the crosslinking process, the molecular weight of the sodium hyaluronate is 1.5 million to 2.5 million Da; Sodium hyaluronate and sodium hydroxide aqueous solution are prepared in a mass-to-volume ratio of 10g:50-100mL. The mass concentration of the sodium hydroxide aqueous solution is 2%; The mass-to-volume ratio of Tween 80 and liquid paraffin is 7g:370-400mL; The volume ratio of oil phase to water phase is 380-400:510-560; The aqueous phase is added over a period of 40-50 minutes. The volume ratio of the homogenized liquid to 1,4-butanediol diglycidyl ether was 900-950:7-8. The mixing process involves ball milling kaolin and sodium hyaluronate to obtain modified kaolin; then mixing the modified kaolin, sodium hyaluronate microspheres, and deionized water, stirring, centrifuging, collecting the precipitate, washing and drying the precipitate to obtain cross-linked microspheres. In the mixture, the mass ratio of kaolin to sodium hyaluronate is 30:10-12; The mass-to-volume ratio of modified kaolin, sodium hyaluronate microspheres, and deionized water is 20-22g:35-40g:900-1000mL; The molecular weight of the sodium hyaluronate is 200,000 Da; The preparation of the mixed bacterial solution involves mixing lactobacillus bacterial solution and yeast bacterial solution at a volume ratio of 1:1-1.5 to obtain a mixed bacterial solution. The bacterial count of the Lactobacillus culture is 1×10⁻⁶. 9 -7×10 9 CFU / mL; The yeast culture solution has a bacterial count of 6 × 10⁻⁶. 8 -1×10 9 CFU / mL; The fermentation process involves inoculating the mixed bacterial solution into the fermentation medium at an inoculation rate of 2-3%, and fermenting at 36-38℃ for 60-70 hours to obtain the fermentation broth. The mixture is prepared by mixing fermentation broth, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine, and deionized water, and then homogenizing the mixture to obtain a compound bacterial fermentation compound Chinese medicine scalp care composition. In the mixture, the mass ratio of fermentation broth, sodium hyaluronate, glycerol, phenoxyethanol, ethylhexylglycerol, butylene glycol, stabilizer, dodecyl betaine, and deionized water is 20-30:1.5-2:3-5:0.5-1:0.01-1:4-5:3-3.5:1.5-2:75-80. The molecular weight of the sodium hyaluronate is 100,000 Da; In the preparation of the stabilizer, the mass ratio of betaine, acetylated sodium hyaluronate, and kaolin is 20-25:12-15:90-100. The degree of acetyl substitution of the acetylated sodium hyaluronate is 3; The stabilizer is prepared by mixing betaine, acetylated sodium hyaluronate, and kaolin, and then ball milling the mixture to obtain the stabilizer.

2. The application of the compound herbal scalp care composition fermented by compound bacteria as described in claim 1.

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