Composition containing ergothioneine and having effects of relieving itching, relieving and repairing and application of composition

Abstract

The invention belongs to the technical field of cosmetics, and provides a composition containing ergothioneine and having the effects of relieving itching, relieving and repairing and application of the composition. The composition is prepared from the following components in percentage by mass: 0.01 to 1 percent of ergothioneine, 0.1 to 4 percent of alpha-glucan polysaccharide, 0.001 to 1 percent of mussel extract and 0.01 to 1 percent of sodium hyaluronate. The active ingredients in the composition cooperate with one another, so that the quick and long-acting itching relieving effect can be achieved, the stability of the active ingredients can be improved to a certain extent, and the effects of the active ingredients are fully exerted.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and provides a composition containing ergothioneine and having antipruritic, soothing and repairing effects and application thereof. Background Art

[0002] When the skin is stimulated by physical or chemical factors such as mosquito bites, itchy skin, redness and sensitivity, or scratching and damage, subjective symptoms such as burning, stinging, itching, and tightness are prone to occur, often accompanied by objective signs such as adverse erythema, scaling, and capillary dilation. In severe cases, redness, swelling, and rashes may occur, accompanied by the trouble of uneven skin color. When the inflammation fades, it is easy to leave marks or spots. For such skin problems, on the premise of basic hydration and moisturizing, it is necessary to strengthen the skin barrier function and thicken the sebum layer, thereby enhancing the skin's resistance and reducing the stimulation of external substances to the skin.

[0003] Especially after mosquito bites, since the mosquito saliva contains a variety of anticoagulant chemicals, red pinpoint-sized petechiae or ecchymoses will appear on the bitten skin. In addition, there are various allergens such as formic acid, natural antigen protein, adenosine deaminase, pathogenic bacteria, etc. in the mosquito saliva. For our body, these are all foreign substances, so the immune system quickly attacks and releases histamine to remove these foreign substances. Histamine will dilate the capillaries, fill the cells with fluid, and cause the skin to become red and swollen. Histamine will also stimulate nerve cells, so there will be an itchy feeling at the bite site.

[0004] Related studies have shown that ergothioneine has the effect of regulating histamine or antihistamine; it can be used as an antipruritic active ingredient. Chinese invention patent application CN117357421A also discloses a composition including ergothioneine and dipeptide-1 or its derivatives, which can effectively reduce the redness, swelling, heat, itching, pain and discomfort of the skin, inhibit inflammatory response, and promote barrier repair; and discloses the use of ergothioneine as a promoter of pro-opiomelanocortin (POMC) expression or β-endorphin production in anti-inflammatory, soothing, skin barrier repair, wound healing, hair growth promotion, anti-aging, and pain relief.

[0005] As one of the "three factors (ectoine, ergothioneine, and bosoepine)", ergothioneine is widely used in whitening and anti-aging products due to its excellent antioxidant properties, but relatively few studies have been conducted on its anti-inflammatory and antipruritic effects. In addition, consumers will choose products based on the addition of certain ingredients, but they cannot verify the stability of the effective ingredients. If the effective raw materials are not stable in the formula, the efficacy of the product cannot be guaranteed. Therefore, while improving the anti-inflammatory, antipruritic, and soothing effects of the product, the stability of the preparation should not be ignored. Summary of the invention

[0006] In view of the problems existing in the prior art, the present invention provides a composition containing ergothioneine with antipruritic, soothing and repairing effects and its application. By combining the active ingredient ergothioneine with other components, the obtained composition has significant anti-inflammatory, antipruritic and soothing effects; in addition, the inventors of the present invention also found during the research process that the presence of alpha-glucan polysaccharide and sodium hyaluronate in the composition and the further addition of hydroxytyrosol can improve the stability of the active ingredient, so that its efficacy can be fully exerted.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0008] The invention provides a composition containing ergothioneine and having antipruritic, soothing and repairing effects. The composition comprises, by mass percentage, 0.01-1% of ergothioneine, 0.1-4% of alpha-glucan polysaccharide, 0.001-1% of mussel extract and 0.01-1% of sodium hyaluronate.

[0009] Preferably, the composition comprises, by mass percentage, 0.1-0.5% ergothioneine, 0.1-2% α-glucan polysaccharide, 0.005-0.05% mussel extract and 0.01-0.05% sodium hyaluronate.

[0010] More preferably, the composition comprises, by mass percentage, 0.3-0.5% ergothioneine, 1-2% α-glucan polysaccharide, 0.01-0.04% mussel extract and 0.02-0.04% sodium hyaluronate.

[0011] In the present invention, the molecular weight of the sodium hyaluronate is ≤100 kDa, preferably ≤10 kDa.

[0012] In the composition of the present invention, ergothioneine can penetrate into the mitochondria of cells, reduce the damage caused by inflammation and oxidative stress, and promote skin repair. Alpha-glucan polysaccharides stimulate the activity of immune cells such as macrophages and natural killer cells, increase the immune function of the skin, enable the skin immune system to better cope with the irritants that cause itching, improve the resistance of the skin itself, play a calming and soothing effect, and can also affect the nerve endings in the skin, regulate the conduction of nerve signals, and reduce the transmission of itching signals. Mussel extract can reduce inflammatory symptoms by inhibiting the generation and release of inflammatory mediators, thereby alleviating itching, and also helps to strengthen the barrier function of the skin, so that the skin can better resist the invasion of external irritants. Alpha-glucan polysaccharides and mussel extracts all have synergistic effects with ergothioneine, and are effective in anti-inflammatory, soothing and repairing. Sodium hyaluronate has moisturizing, anti-inflammatory and soothing effects in skin tissue. At the same time, in promoting wound healing, it is believed that it acts as a protective transport vehicle, bringing peptide growth factors and other structural proteins to the site of action, and then enzymatically degrading and releasing active proteins to promote tissue repair. It can also promote skin repair in combination with ergothioneine; it can also play a role in thickening to stabilize the preparation.

[0013] As a preferred technical solution of the present invention, the composition further comprises: one or more of hydroxytyrosol, menthone glycerol ketal and methyl diisopropyl propionamide.

[0014] Among them, hydroxytyrosol protects skin cells from damage by ultraviolet rays, inhibits the release of histamine, reduces the mRNA expression of inflammatory factors TNF-α and IL-1β, and has an anti-inflammatory effect; it can be used as an acne prevention and treatment ingredient, and can inhibit the release of histamine, and has a good soothing and anti-allergic effect, thereby improving problems such as red blood streaks, irritation and peeling, inflammatory acne, and post-inflammatory pigmentation caused by inflammation, and has a synergistic effect with ergothioneine in anti-inflammatory aspects; menthone glycerol acetal and methyl diisopropylpropionamide, as cooling and soothing ingredients, have a synergistic effect with ergothioneine.

[0015] Further preferably, the composition further comprises, by mass percentage, 0.01-1% of hydroxytyrosol, 0.01-0.8% of menthone glycerol ketal and 0.01-0.5% of methyl diisopropyl propionamide.

[0016] Still more preferably, the composition further comprises, by mass percentage, 0.05-0.5% of hydroxytyrosol, 0.01-0.1% of menthone glycerol ketal and 0.01-0.1% of methyl diisopropyl propionamide.

[0017] In order to improve the stability of the preparation containing any of the above-mentioned compositions, the composition further comprises: one or more of p-hydroxyacetophenone, 1,2-hexanediol, PEG-40 hydrogenated castor oil, polysorbate and butylene glycol.

[0018] Among them, p-hydroxyacetophenone and 1,2-hexanediol have a preservative effect; PEG-40 hydrogenated castor oil, polysorbate and butylene glycol cooperate synergistically to play an emulsifying and solubilizing role, so that the stability of the composition preparation is guaranteed.

[0019] More preferably, the composition comprises, by mass percentage, 0.1-1% p-hydroxyacetophenone, 0.1-5% 1,2-hexanediol, 0.1-2% PEG-40 hydrogenated castor oil, 0.1-1% polysorbate and 0.1-5% butylene glycol.

[0020] The present invention also provides the use of any of the above-mentioned compositions in the preparation of skin care products with antipruritic, soothing and repairing effects.

[0021] Furthermore, the skin care product includes any one of moisturizing water, lotion, essence, moisturizing cream, facial mask liquid and spray.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The composition of the present invention has significant efficacy under the coordination of various active ingredients, and can achieve itching relief within 5 minutes and long-term soothing for 8 hours; wherein ergothioneine, α-glucan polysaccharide and mussel extract have synergistic effects in anti-inflammatory, antipruritic, cooling and soothing, and sodium hyaluronate not only moisturizes and soothes, but also brings anti-inflammatory and repairing effects to the composition; under the action of hydroxytyrosol and ergothioneine, its anti-inflammatory effect is further improved; under the coordination of menthone glycerol ketal and methyl diisopropyl propionamide with ergothioneine, the soothing effect is further improved;

[0024] (2) If the active ingredient is unstable, the long-term placement of the efficacy component gradually decreases, resulting in the product efficacy not being guaranteed for a long time. The composition of the present invention contains ergothioneine, which has a strong effect, but it will degrade under conditions such as light and heat, and the smell of trimethylamine will appear, affecting the efficacy and quality of the product. The inventors found that in the composition of the present invention, the presence of α-glucan polysaccharide and sodium hyaluronate can effectively guarantee the stability of ergothioneine; in addition, by further adding hydroxytyrosol, its light and heat stability is also improved to a certain extent, and the comprehensive performance of the composition of the present invention is more excellent;

[0025] (3) The composition is fragrance-free, hormone-free, mild and non-irritating, and the preparation process is simple and efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The test results of the transepidermal water loss value of the antipruritic spray prepared in Example 1 ( * p<0.05, compared with 0min / 0h, there is a significant difference);

[0027] Figure 2 The test results of the antipruritic spray skin hemoglobin content prepared in Example 1 ( * p<0.05, compared with 0min / 0h, there is a significant difference);

[0028] Figure 3 The a value test result of the skin red zone of the antipruritic spray prepared in Example 1 ( * p<0.05, compared with 0min / 0h, there is a significant difference);

[0029] Figure 4 The test results of the lactic acid stinging level of the antipruritic spray prepared in Example 1 ( * p<0.05, compared with 0h, there is a significant difference);

[0030] Figure 5 This is a comparison chart of the effects before and after using the antipruritic spray of Example 1 in a mosquito bite test;

[0031] Figure 6 The comparison chart of the antipruritic spray effects of Example 1 and Comparative Examples 5-9 was used in the antipruritic spray population efficacy test. DETAILED DESCRIPTION

[0032] The embodiments of the present invention will be described in detail below in conjunction with examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. In the examples, if no specific experimental steps or conditions are indicated, the operation or conditions of the conventional experimental steps described in the literature in this area can be carried out. If the manufacturer is not indicated for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially, and the sources of the raw materials used are shown in Table 1.

[0033] Table 1

[0034]

[0035]

[0036] Examples 1-5

[0037] The raw materials were mixed and homogenized according to the formula in Table 2 to obtain the antipruritic spray.

[0038] Table 2 Composition formula table (unit: wt%)

[0039]

[0040] Comparative Examples 1-6

[0041] The raw materials were mixed and homogenized according to the formula in Table 3 to obtain the antipruritic spray.

[0042] Table 3 Composition formula table (unit: wt%)

[0043]

[0044]

[0045] Comparative Examples 7-9

[0046] The raw materials were mixed and homogenized according to the formula in Table 4 to obtain the antipruritic spray.

[0047] Table 4 Composition formula table (unit: wt%)

[0048]

[0049] Test Example 1: Anti-itching Spray Efficacy Test

[0050] The anti-inflammatory and soothing effects of the antipruritic sprays prepared in the above-mentioned comparative examples were tested.

[0051] 65 subjects aged 20-55 years old were randomly selected, with a male-female ratio of 1:1, and randomly divided into 20 groups, each with 5 people. The spray prepared in each comparative example was tested for transepidermal water loss value, skin hemoglobin content, skin red zone a value and lactic acid stinging level, and the average value of the test results of 5 people was taken. Among them, the transepidermal water loss value, skin hemoglobin content, skin red zone a value and lactic acid stinging level of the spray of Example 1 measured at different time points are shown in Figure 1-4 .

[0052] according to Figure 1-4 The results show that compared with 0min, after using the antipruritic spray of the embodiment of the present invention for 5min, 15min and 30min, the transepidermal water loss values ​​of the five subjects decreased significantly; compared with 0min / 0h, after using the antipruritic spray of the embodiment of the present invention for 5min, 15min, 30min and 8h, the skin hemoglobin content and the skin red zone a value of the five subjects decreased significantly; compared with 0h, after using the antipruritic spray of the embodiment of the present invention for 5min, 15min, 30min and 8h, the skin hemoglobin content of the five subjects decreased significantly; compared with 0min / 0h, after using the antipruritic spray of the embodiment of the present invention for 8h, the stinging score and the total sensation score of the five subjects decreased significantly.

[0053] Compared with 0min / 0h, the statistical results of transepidermal water loss, skin hemoglobin content and skin red zone a value after 5min, 15min, 30min and 8h, and the change rate of lactic acid stinging level after 8h are shown in Tables 6-9.

[0054] Table 6 Transepidermal water loss value change rate results (%)

[0055]

[0056] Note: Transepidermal water loss value change rate = (Xmin-0min) / 0min×100% (positive value indicates increase, negative value indicates decrease).

[0057] Table 7 Skin hemoglobin content test results

[0058]

[0059] Note: Change rate of skin hemoglobin content = (Xmin-0min) / 0min×100% or (Xh-0h) / 0h×100% (positive value indicates increase, negative value indicates decrease).

[0060] Table 8 Skin red zone a value test results

[0061]

[0062]

[0063] Note: The rate of change of the a value of the skin red zone = (Xmin-0min) / 0min×100% or (Xh-0h) / 0h×100% (positive value indicates increase, negative value indicates decrease).

[0064] Table 9 Lactic acid stinging grade results

[0065] Group Pain score Overall feeling score Example 1 -41.18％ -47.83％ Example 2 -40.49％ -45.52％ Example 3 -38.24％ -44.33％ Example 4 -40.35％ -42.44％ Example 5 -40.21％ -41.38％ Comparative Example 1 -35.36％ -40.21％ Comparative Example 2 -25.42％ -30.16％ Comparative Example 3 -30.25％ -36.31％ Comparative Example 4 -24.22％ -35.12％ Comparative Example 5 -37.15％ -40.21％ Comparative Example 6 -28.76％ -32.95％ Comparative Example 7 -30.56％ -40.21％ Comparative Example 8 -35.33％ -37.42％ Comparative Example 9 -33.19％ -36.03％

[0066] Note: Lactic acid stinging level change rate = (Xh-0h) / 0h×100% (positive value indicates increase, negative value indicates decrease).

[0067] According to the above data, it can be concluded that: Comparative Examples 1-6 and Example 5 and Example 1 show that α-glucan polysaccharide, mussel extract, hydroxytyrosol and ergothioneine cooperate synergistically to form a skin barrier and anti-inflammatory and soothing effects; and in the presence of hydroxytyrosol, menthone glycerol ketal and methyl isopropyl propionamide, the corresponding effect is further improved; in addition, according to Comparative Examples 7 and 8 compared with Example 1, menthone glycerol ketal and methyl isopropyl propionamide have an enhancing effect in forming a skin barrier, anti-inflammatory and soothing, and relieving lactic acid stinging; according to Comparative Example 9 compared with Example 1, the combination of the two and ergothioneine also have an enhancing effect in relieving lactic acid stinging.

[0068] Test Example 2: Mosquito bite test

[0069] 25 individuals bitten by mosquitoes were recruited and randomly divided into 5 groups. The antipruritic sprays of Example 1 and Comparative Examples 1-4 of the present invention were sprayed or applied on the mosquito bite sites. The antipruritic time (min) was recorded. The results are shown in Table 10. Photos were taken to compare the skin conditions before and after use (30 minutes after use). The typical case (skin conditions before and after applying the antipruritic spray of Example 1) is shown in Table 10. Figure 5 .

[0070] Table 10 Mosquito bite test results

[0071] Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 1 3 13 10 15 10 2 4.5 15 11 13 9 3 3.5 12 9 12 10 4 4 12 12 15 8 5 4 13 14 14 9 Average time 3.8 13 11.2 13.8 9.2

[0072] Test Example 3: Antipruritic Spray Population Efficacy Test

[0073] 10 individuals who react to the itching caused by histamine were recruited, and two forearms were used as test points. Each test site was wet-compressed with 0.2% histamine (solvent was saline) for 10-15 minutes, and redness, swelling and itching of the test points were induced by the experiment. After using the antipruritic spray of Example 1 and Comparative Examples 5-9 for 10-15 minutes, photos were taken for comparison. Typical cases are shown in Figure 6 Shown

[0074] Comparative Examples 10-12

[0075] The raw materials were mixed and homogenized according to the formula in Table 11 to obtain an antipruritic spray.

[0076] Table 11 Composition formula table (unit: wt%)

[0077]

[0078]

[0079] Test Example 4: Antipruritic Spray Stability Test

[0080] (1) Light stability test:

[0081] A transparent glass bottle with a cylindrical diameter of about 5 cm was used. The composition samples prepared in Example 1 and Comparative Examples 10-12 were placed in the glass bottles and the bottle caps were tightly closed. The bottles were continuously illuminated for 14 days in a lighting test light box. The test light box was equipped with a group of lamp tubes. The samples were about 30-40 cm away from the lamp tubes. A lamp tube suitable for this purpose is POLARIT (daylight type). The 40-watt (Thorn-EMI) lamp tube is 132 cm long. A group of 12 lamp tubes provided light intensity and spectral distribution simulating sunlight.

[0082] (2) High temperature stability test:

[0083] The composition samples prepared in Example 1 and Comparative Examples 10-12 were poured into glass bottles, the bottle caps were tightly closed, and the bottles were placed in an Incucell incubator at 50° C. for 14 days to observe the changes in the products.

[0084] The composition samples prepared in Example 1 and Comparative Examples 10-12 and their ergothioneine content after light and high temperature treatment were tested by ultra-high performance liquid chromatography (test standard: NY / T 3872-2021), and the test results are shown in Table 12.

[0085] Table 12 Antipruritic spray stability test results

[0086]

[0087] As can be seen from the above table, the α-glucan polysaccharide, sodium hyaluronate and hydroxytyrosol in the composition of the present invention not only have corresponding effects, but also play a certain role in the stability of ergothioneine.

[0088] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing ergothioneine having an antipruritic, soothing and repairing effect, characterized in that: The composition comprises, by mass percentage, 0.01-1% of ergothioneine, 0.1-4% of α-glucan polysaccharide, 0.001-1% of mussel extract and 0.01-1% of sodium hyaluronate.

2. The composition according to claim 1, characterized in that The composition comprises, by mass percentage, 0.1-0.5% of ergothioneine, 0.1-2% of α-glucan polysaccharide, 0.005-0.05% of mussel extract and 0.1-0.5% of sodium hyaluronate.

3. The composition according to claim 2, characterized in that The composition comprises, by mass percentage, 0.3-0.5% of ergothioneine, 1-2% of α-glucan polysaccharide, 0.01-0.04% of mussel extract and 0.2-0.4% of sodium hyaluronate.

4. The composition according to claim 1, characterized in that The composition further comprises: one or more of hydroxytyrosol, menthone glycerol ketal and methyl diisopropyl propionamide.

5. The composition according to claim 4, characterized in that The composition further comprises, by mass percentage, 0.01-1% of hydroxytyrosol, 0.01-0.8% of menthone glycerol ketal and 0.01-0.5% of methyl diisopropyl propionamide.

6. The composition according to claim 5, characterized in that The composition further comprises, by mass percentage, 0.05-0.5% of hydroxytyrosol, 0.01-0.1% of menthone glycerol ketal and 0.01-0.1% of methyl diisopropyl propionamide.

7. The composition according to any one of claims 1 to 6, characterized in that The composition further comprises: one or more of p-hydroxyacetophenone, 1,2-hexanediol, PEG-40 hydrogenated castor oil, polysorbate and butylene glycol.

8. The composition according to claim 7, characterized in that The composition comprises, by mass percentage, 0.1-1% of p-hydroxyacetophenone, 0.1-5% of 1,2-hexanediol, 0.1-2% of PEG-40 hydrogenated castor oil, 0.1-1% of polysorbate and 0.1-5% of butanediol.

9. Use of the composition according to any one of claims 1 to 8 in the preparation of skin care products with antipruritic, soothing and repairing effects.

10. The use according to claim 9, characterized in that: The skin care product includes any one of moisturizing water, emulsion, essence, moisturizing cream, facial mask liquid and spray.

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