A method for improving the purity of active collagen by using ultrasonic-assisted extraction technology

Abstract

The present invention relates to the technical field of collagen detection, and particularly to a method for improving the purity of active collagen by using ultrasonic-assisted extraction technology. The present invention determines whether the bubble release fluid space of the collagen solution is uniform according to the fluctuation degree of the liquid level height of the collagen solution, determines the abnormal interval segment inside the fluid according to the curve segment coincidence degree of the absorption spectral curves of different layer samples in several wavelength interval segments, determines whether there is an obvious fluid stratification and polymerization phenomenon in the collagen solution by calculating the fluid stratification polymerization characterization value. Finally, the ultrasonic extraction process of the collagen solution is adjusted according to the overall curve coincidence degree of the absorption spectral curves of different layer samples after stirring, excluding the interference of the stratification phenomenon of the active collagen solution caused by the difference in the hydrodynamic state inside on the purity detection of the collagen solution, and effectively adjusting the extraction process of the collagen solution according to the accurate result of the purity detection of the collagen solution.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen detection, and particularly relates to a method for improving the purity of active collagen by using ultrasonic-assisted extraction technology. Background Art

[0002] As an important biomaterial, the research and application of active collagen are developing rapidly. The preparation process of active collagen mainly involves extraction methods such as hot water extraction, acid-base hydrolysis, and enzymatic hydrolysis. With the development of extraction technology and consumers' pursuit of high-quality life, active collagen has broad application prospects in the fields of medical aesthetics, beauty, and health care.

[0003] Active collagen solution has a wide range of applications in the fields of medicine, beauty, and skin care. In order to ensure that the extracted active collagen solution has a higher purity performance, the existing ultrasonic-assisted extraction technology can significantly increase the yield of target components. The triple helix structure of active collagen molecules causes internal differences in the hydrodynamic properties of active collagen solution, which affects the accurate detection of the purity of collagen solution. Therefore, it is particularly important to detect and analyze the parameters with data representativeness for the purity performance during the extraction process of active collagen solution.

[0004] For example, Chinese Patent Publication No.: CN117951484A, which discloses a method and system for detecting the quality of collagen tripeptide. The method first divides the product sampling data into product group data, performs bilateral filtering on each product group data to obtain each segmented filtering data, and then obtains the product filtering data. During the process of calculating the adjusted weight of each reference data point in the bilateral filtering, the initial weight is adjusted according to the data reliability value of the reference data point to obtain the adjusted weight of the reference data point. According to the absorption spectrum filtering curves of all sampling samples, the quality detection result of collagen tripeptide is obtained.

[0005] The following problems still exist in the prior art:

[0006] The prior art does not consider the interference of the layering phenomenon caused by the internal hydrodynamic state differences in the extracted active collagen solution on the accuracy of collagen solution purity detection, and the distorted collagen solution purity detection result affects the effectiveness of adjusting the collagen solution during the extraction process. Summary of the Invention

[0007] Therefore, the present invention provides a method for improving the purity of active collagen by using ultrasonic-assisted extraction technology to overcome the problems that the prior art cannot eliminate the interference of the layering phenomenon of active collagen solution caused by internal hydrodynamic state differences on the collagen solution purity detection, and cannot effectively adjust the extraction process of collagen solution.

[0008] To achieve the above object, the present invention provides a method for improving the purity of active collagen by using ultrasonic-assisted extraction technology, including:

[0009] Filling the collagen solution extracted by ultrasound into a number of packaging bottles, and determining whether the bubble release fluid space of the collagen solution is uniform based on the degree of fluctuation of the liquid level height of the collagen solution in the packaging bottles;

[0010] In response to the non-uniformity of the bubble release fluid space of the collagen solution, obtaining the sampling information after a preset standing time of the collagen solution, where the sampling information includes the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample;

[0011] Determining the curve segment coincidence degree of the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample in a number of wavelength interval segments, and determining the wavelength interval segment where the curve segment corresponding to the curve segment coincidence degree that does not meet the preset standard is located as the abnormal interval segment inside the fluid;

[0012] Calculating a fluid stratification aggregation characterization value based on the number of the abnormal interval segments inside the fluid and the curve coincidence degree corresponding to each abnormal interval segment inside the fluid to determine whether there is an obvious fluid stratification aggregation phenomenon in the collagen solution;

[0013] In response to the obvious fluid stratification aggregation phenomenon in the collagen solution, obtaining the overall curve coincidence degree of the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample after stirring for a preset time of the collagen solution, and determining whether the stratification aggregation phenomenon of the collagen solution is abnormal according to the overall curve coincidence degree.

[0014] Further, the process of determining the degree of fluctuation of the liquid level height of the collagen solution in the packaging bottle is as follows:

[0015] Obtaining the liquid level height of the collagen solution in a number of packaging bottles, and calculating the standard deviation of the liquid level height according to the liquid level height of the collagen solution in the number of packaging bottles.

[0016] Further, the process of determining whether the bubble release fluid space of the collagen solution is uniform includes:

[0017] Comparing the standard deviation of the liquid level height with a preset standard deviation threshold of the liquid level height;

[0018] If the standard deviation of the liquid level height is greater than the standard deviation threshold of the liquid level height, it is determined that the bubble release fluid space of the collagen solution is non-uniform.

[0019] Further, the process of obtaining the sampling information includes:

[0020] Collect the upper-layer collagen solution sample and the lower-layer collagen solution sample of the collagen solution after the preset static time;

[0021] Use a visible spectrometer to measure the absorption spectra of the upper-layer collagen solution sample and the lower-layer collagen solution sample;

[0022] With the wavelength of the light emitted by the visible spectrometer as the horizontal axis and the absorbance as the vertical axis, determine the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample.

[0023] Further, the steps for determining the overlap degree of the curve segments include:

[0024] Divide the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample into several wavelength interval segments in the direction of the horizontal axis at the same preset wavelength interval;

[0025] Determine the absorption spectral curve segment of the upper-layer collagen solution sample and the absorption spectral curve segment of the lower-layer collagen solution sample within each wavelength interval segment;

[0026] Calculate the overlap degree of the absorption spectral curve segment of the upper-layer collagen solution sample and the absorption spectral curve segment of the lower-layer collagen solution sample within the wavelength interval segment, and determine the overlap degree as the overlap degree of the curve segments.

[0027] Further, the process for determining the abnormal interval segment inside the fluid is:

[0028] Compare the overlap degree of the curve segments with a preset reference value of the overlap degree of the curve segments. If the overlap degree of the curve segments is less than or equal to the reference value of the overlap degree of the curve segments, determine that the wavelength interval segment where the curve segment corresponding to the overlap degree of the curve segments is located is the abnormal interval segment inside the fluid.

[0029] Further, calculate the fluid stratification polymerization characterization value according to the following formula:

[0030]

[0031] where M is the fluid stratification polymerization characterization value, k is the ratio of the number of abnormal interval segments inside the fluid to the number of wavelength interval segments, k0 is a preset reference value of the quantity ratio, R av is the average value of the curve overlap degrees corresponding to each abnormal interval segment inside the fluid, R0 is a preset reference value of the average value of the curve overlap degrees, α is a preset weight coefficient of the quantity ratio, β is a preset weight coefficient of the curve overlap degree, and e is a constant.

[0032] Further, the process for determining whether there is an obvious fluid stratification polymerization phenomenon in the collagen solution includes:

[0033] Compare the layered polymerization characterization value of the fluid with a preset threshold value of the layered polymerization characterization value of the fluid;

[0034] If the layered polymerization characterization value of the fluid is greater than the threshold value of the layered polymerization characterization value of the fluid, it is determined that there is an obvious phenomenon of layered polymerization of the collagen solution.

[0035] Further, the process of obtaining the overall coincidence degree of the curve includes:

[0036] Collect the upper-layer collagen solution sample and the lower-layer collagen solution sample of the collagen solution after stirring for a preset duration;

[0037] Use a visible spectrometer to measure the absorption spectra of the upper-layer collagen solution sample and the lower-layer collagen solution sample, and determine the absorption spectrum curves of the upper-layer collagen solution sample and the lower-layer collagen solution sample;

[0038] Calculate the coincidence degree of the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample, and determine the coincidence degree as the overall coincidence degree of the curve.

[0039] Further, the process of adjusting the ultrasonic power and the ultrasonic duration during the ultrasonic extraction of the collagen solution includes:

[0040] Compare the overall coincidence degree of the curve with a preset reference value of the overall coincidence degree of the curve;

[0041] If the overall coincidence degree of the curve is greater than or equal to the reference value of the overall coincidence degree of the curve, it is determined that the ultrasonic extraction of the collagen solution is not adjusted;

[0042] If the overall coincidence degree of the curve is less than the reference value of the overall coincidence degree of the curve, adjust the ultrasonic power and the ultrasonic duration during the ultrasonic extraction of the collagen solution;

[0043] Wherein, the ultrasonic power has a negative correlation with the overall coincidence degree of the curve, and the ultrasonic duration has a negative correlation with the overall coincidence degree of the curve.

[0044] Compared with the prior art, the beneficial effects of the present invention are as follows. The present invention determines whether the bubble release fluid space of the collagen solution is uniform according to the fluctuation degree of the liquid level height of the collagen solution in several packaging bottles, determines the curve segment coincidence degree of the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample in several wavelength intervals according to the sampling information of the collagen solution after a preset standing time to determine the abnormal interval segment inside the fluid, calculates the fluid stratification and aggregation characterization value according to the number of abnormal interval segments inside the fluid and the corresponding curve coincidence degree to determine whether there is an obvious fluid stratification and aggregation phenomenon in the collagen solution. Finally, the ultrasonic extraction process of the collagen solution is adjusted according to the overall curve coincidence degree of the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample of the collagen solution after stirring for a preset time. Furthermore, it realizes determining the hydrodynamic state of the collagen solution according to the release situation of the bubbles inside the collagen solution, excluding the interference of the stratification phenomenon of the active collagen solution caused by the internal difference in the hydrodynamic state on the purity detection of the collagen solution, and effectively adjusting the extraction process of the collagen solution according to the accurate result of the purity detection of the collagen solution.

[0045] In particular, the present invention determines whether the bubble release fluid space of the collagen solution is uniform according to the fluctuation degree of the liquid level height of the collagen solution filled in several packaging bottles. Those skilled in the art can understand that the triple helix structure of the active collagen molecule causes the collagen solution to be in a viscous state. The hydrodynamic state generated by the viscous liquid fluid during the filling process has internal differences, resulting in uneven distribution of the bubbles generated in the collagen solution during the filling process. The different rates of bubble precipitation from the collagen solution due to the uneven position distribution of the bubbles lead to different degrees of liquid level drop of the collagen solution after the bubbles precipitate. The greater the fluctuation degree of the liquid level height of the collagen solution after filling, the greater the internal difference in the hydrodynamic state of the collagen solution. Furthermore, it realizes determining the hydrodynamic state of the collagen solution according to the release situation of the bubbles inside the collagen solution, improving the detection accuracy.

[0046] In particular, after the collagen solution of the present invention is allowed to stand for a preset standing time, the curve segment coincidence degree of the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample in several wavelength intervals is determined. Those skilled in the art can understand that if there are impurities in the collagen solution or the aggregation distribution of protein molecules is uneven, during the standing process, the impurity concentration or protein molecule concentration of the upper-layer collagen solution sample and the lower-layer collagen solution sample of the collagen solution will be different. The impurities or collagen molecules in different aggregation states affect the absorbance of the collagen solution sample at specific wavelengths. By calculating the curve segment coincidence degree of the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample in several wavelength intervals, the present invention can clearly determine the purity difference between the upper-layer collagen solution sample and the lower-layer collagen solution sample. Furthermore, accurate detection of collagen solutions with different hydrodynamic states is achieved.

[0047] In particular, the present invention calculates the fluid stratification polymerization characterization value through the number of abnormal intervals inside the fluid and the curve coincidence degree corresponding to each abnormal interval inside the fluid. During the actual comparison of the curve segment coincidence degree of the absorption spectral curves of the collagen solution samples, the more the number of wavelength intervals where the curve segment coincidence degree does not meet the preset standard, the greater the difference degree between the upper-layer collagen solution sample and the lower-layer collagen solution sample. Similarly, the smaller the average value of the curve coincidence degrees corresponding to several abnormal intervals inside the fluid, the greater the difference degree between the upper-layer collagen solution sample and the lower-layer collagen solution sample. Furthermore, the stratification difference degree of the collagen solution samples is visually quantified, the detection accuracy of collagen solutions with different hydrodynamic states is improved, and the interference of the stratification phenomenon of the active collagen solution caused by the difference in hydrodynamic state inside on the purity detection of the collagen solution is excluded.

[0048] In particular, the present invention adjusts the ultrasonic extraction process of the collagen solution according to the overall curve coincidence degree between the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample after stirring the collagen solution for a predetermined duration. Those skilled in the art can understand that if the stratification difference of the collagen solution sample is caused by different aggregation concentrations of protein molecules due to the difference in the hydrodynamic state of the collagen solution, by stirring the collagen solution, the stratification difference of the collagen solution sample can be significantly reduced, that is, the overall curve coincidence degree between the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample is relatively high, then the stratification and aggregation phenomenon of the collagen solution is not caused by impurities, and there is no need to adjust the ultrasonic extraction of the collagen solution. If the stratification difference of the collagen solution sample is caused by impurities in the collagen solution, by stirring the collagen solution, the stratification difference of the collagen solution sample cannot be significantly reduced, that is, the overall curve coincidence degree between the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample is relatively low. It is necessary to increase the ultrasonic power during the ultrasonic extraction process of the collagen solution and extend the ultrasonic duration during the ultrasonic extraction process of the collagen solution to improve the purity of the collagen solution during the extraction process. Furthermore, it is necessary to exclude the interference of the stratification phenomenon of the active collagen solution caused by the difference in the hydrodynamic state inside on the purity detection of the collagen solution, and effectively adjust the extraction process of the collagen solution according to the accurate results of the purity detection of the collagen solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 FIG. is a flowchart of the method steps for improving the purity of active collagen by using ultrasonic-assisted extraction technology in an embodiment of the present invention;

[0050] Figure 2 FIG. is a flowchart of the steps for obtaining sampling information in an embodiment of the present invention;

[0051] Figure 3 FIG. is a flowchart of the steps for determining the curve segment coincidence degree in an embodiment of the present invention;

[0052] Figure 4 FIG. is a logic flowchart for determining whether there is an obvious fluid stratification and aggregation phenomenon in the collagen solution in an embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0053] In order to make the objectives and advantages of the present invention clearer and more understandable, the present invention will be further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0054] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of the present invention and do not limit the protection scope of the present invention.

[0055] It should be noted that in the description of the present invention, the terms indicating the direction or positional relationship such as "upper", "lower", "inner", "outer", etc. are based on the direction or positional relationship shown in the drawings. This is only for the convenience of description, rather than indicating or implying that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention.

[0056] Please refer to Figure 1 As shown, it is a method step diagram for improving the purity of active collagen by using ultrasonic-assisted extraction technology in an embodiment of the present invention. A method for improving the purity of active collagen by using ultrasonic-assisted extraction technology of the present invention includes:

[0057] Step S100: Fill the collagen solution extracted by ultrasound into a number of packaging bottles, and determine whether the bubble release fluid space of the collagen solution is uniform based on the fluctuation degree of the liquid level height of the collagen solution in the packaging bottles;

[0058] Step S200: In response to the non-uniform bubble release fluid space of the collagen solution, obtain the sampling information after a preset standing time of the collagen solution. The sampling information includes the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample;

[0059] Step S300: Determine the curve segment coincidence degree of the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample in a number of wavelength interval segments, and determine the wavelength interval segment where the curve segment corresponding to the curve segment coincidence degree that does not meet the preset standard is located as the abnormal interval segment inside the fluid;

[0060] Step S400: Calculate the fluid stratification aggregation characterization value based on the number of the abnormal interval segments inside the fluid and the curve coincidence degree corresponding to each abnormal interval segment inside the fluid, so as to determine whether there is an obvious fluid stratification aggregation phenomenon in the collagen solution;

[0061] Step S500: In response to the obvious fluid stratification aggregation phenomenon in the collagen solution, obtain the overall curve coincidence degree of the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample after stirring for a preset time of the collagen solution, and determine whether the stratification aggregation phenomenon of the collagen solution is abnormal according to the overall curve coincidence degree.

[0062] Specifically, those skilled in the art can understand that the ultrasonic-assisted extraction technology can improve the extraction efficiency and purity of collagen in the extraction of active collagen. By mixing the processed raw materials with an appropriate amount of solvent and performing ultrasonic treatment on the mixed solvent at a preset ultrasonic power for a preset ultrasonic duration to promote the release of collagen, and separating the solvent after ultrasonic treatment and enzymatic hydrolysis by centrifugation to obtain a collagen solution. The ultrasonic power and ultrasonic duration are parameters commonly used by those skilled in the art in the ultrasonic-assisted extraction technology and will not be elaborated here.

[0063] Specifically, in the embodiments of the present invention, the liquid level height of the collagen solution filled in several packaging bottles can be obtained through a vision system. The vision system with a high-definition camera is widely used in the detection of liquid level height in the food and pharmaceutical industries. This is prior art and will not be elaborated here.

[0064] In the embodiments of the present invention, the preset standing duration of the collagen solution is preset by those skilled in the art according to the standing duration required for the internal substances of the collagen solution to be layered. Preferably, the value range of the preset standing duration can be [1, 6], and the interval unit is h.

[0065] Specifically, in the embodiments of the present invention, the Euclidean distance between corresponding points on two curves can be calculated through existing algorithms, and then the distances are summed up. The smaller the obtained value, the higher the degree of coincidence between the two curves. Based on this, the curve segment coincidence degree of the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample in several wavelength intervals and the overall curve coincidence degree of the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample can be calculated. The calculation of the curve coincidence degree is a commonly used technical means in curve analysis and will not be elaborated here.

[0066] Specifically, the process of determining the fluctuation degree of the liquid level height of the collagen solution in the packaging bottle is as follows:

[0067] Obtain the liquid level height of the collagen solution in several packaging bottles, and calculate the liquid level height standard deviation h based on the liquid level height of the collagen solution in several packaging bottles c 。

[0068] Specifically, the process of determining whether the bubble release fluid space of the collagen solution is uniform includes:

[0069] Compare the liquid level height standard deviation h c with a preset liquid level height standard deviation threshold h c0 for comparison;

[0070] If the liquid level height standard deviation h c is less than or equal to the liquid level height standard deviation threshold hc0 , it is determined that the bubble release fluid space of the collagen solution is uniform;

[0071] If the standard deviation of the liquid level height h c is greater than the standard deviation threshold of the liquid level height h c0 , it is determined that the bubble release fluid space of the collagen solution is non-uniform.

[0072] Preferably, in the embodiments of the present invention, the standard deviation threshold of the liquid level height h c0 is determined according to the height value H of the packaging bottle, and h c0 = δ×H, where δ is the value factor of the standard deviation threshold of the liquid level height, and the value range of δ is [0.08, 0.1].

[0073] Specifically, the present invention determines whether the bubble release fluid space of the collagen solution is uniform according to the fluctuation degree of the liquid level height of the collagen solution filled in several packaging bottles. Those skilled in the art can understand that the triple helix structure of active collagen molecules causes the collagen solution to be in a viscous state, and there are internal differences in the hydrodynamic state generated by the viscous liquid fluid under impact during the filling process, resulting in uneven distribution of the bubbles generated in the collagen solution during the filling process. The different rates of bubble precipitation from the collagen solution with uneven position distribution lead to different degrees of liquid level drop of the collagen solution after bubble precipitation. The greater the fluctuation degree of the liquid level height of the filled collagen solution, the greater the internal difference in the hydrodynamic state of the collagen solution. Furthermore, it realizes determining the hydrodynamic state of the collagen solution according to the release situation of the internal bubbles of the collagen solution, improving the detection accuracy.

[0074] Specifically, please refer to Figure 2 shown in the figure, which is a step diagram for obtaining sampling information in the embodiments of the present invention. The process of obtaining the sampling information includes:

[0075] Step S201, collecting the upper-layer collagen solution sample and the lower-layer collagen solution sample of the collagen solution after a preset standing time;

[0076] Step S202, using a visible spectrometer to measure the absorption spectra of the upper-layer collagen solution sample and the lower-layer collagen solution sample;

[0077] Step S203, taking the wavelength of the light emitted by the visible spectrometer as the horizontal axis and the absorbance as the vertical axis to determine the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample.

[0078] Specifically, in the embodiments of the present invention, the absorption of the collagen solution to light of a specific wavelength can be measured by an ultraviolet-visible spectrometer, which is prior art and will not be elaborated here.

[0079] Specifically, please refer to Figure 3 As shown, it is a step diagram for determining the curve segment coincidence degree in an embodiment of the present invention. The steps for determining the curve segment coincidence degree include:

[0080] Step S301: Divide the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample into several wavelength interval segments in the horizontal axis direction at the same preset wavelength interval;

[0081] Step S302: Determine the absorption spectrum curve segment of the upper-layer collagen solution sample and the absorption spectrum curve segment of the lower-layer collagen solution sample within each wavelength interval segment;

[0082] Step S303: Calculate the coincidence degree between the absorption spectrum curve segment of the upper-layer collagen solution sample and the absorption spectrum curve segment of the lower-layer collagen solution sample within the wavelength interval segment, and determine the coincidence degree as the curve segment coincidence degree.

[0083] Preferably, in the embodiment of the present invention, the value range of the preset wavelength interval is [20, 30], and the interval unit is nm.

[0084] Specifically, in the embodiment of the present invention, the Euclidean distance between corresponding points on the two curves can be calculated through existing algorithms, and then the sum of all distances is calculated. The smaller the obtained value, the higher the curve segment coincidence degree. The calculation of the curve segment coincidence degree is a commonly used technical means in curve analysis and will not be elaborated here.

[0085] Specifically, after the collagen solution is left standing for a preset standing time in the present invention, the curve segment coincidence degree between the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample within several wavelength interval segments is determined. Those skilled in the art can understand that if there are impurities inside the collagen solution or the aggregation distribution of protein molecules is uneven, during the standing process, the impurity concentration or protein molecule concentration of the upper-layer collagen solution sample and the lower-layer collagen solution sample of the collagen solution will show differences. The impurities or collagen molecules in different aggregation states affect the absorbance of the collagen solution sample at a specific wavelength. By calculating the curve segment coincidence degree between the absorption spectrum curve of the upper-layer collagen solution sample and the absorption spectrum curve of the lower-layer collagen solution sample within several wavelength interval segments in the present invention, the purity difference between the upper-layer collagen solution sample and the lower-layer collagen solution sample can be clearly determined. Furthermore, accurate detection of collagen solutions with different hydrodynamic states is achieved.

[0086] Specifically, the process of determining the abnormal interval segment inside the fluid is as follows:

[0087] Compare the curve segment coincidence degree R with the preset curve segment coincidence degree reference value Ra Make a comparison. If the coincidence degree R of the curve segment is less than or equal to the reference value R of the coincidence degree of the curve segment a , then it is determined that the wavelength interval segment where the curve segment corresponding to the coincidence degree of the curve segment is located is the abnormal interval segment inside the fluid.

[0088] Preferably, in the embodiment of the present invention, the preset reference value R of the coincidence degree of the curve segment a has a value range of [88%, 93%].

[0089] Specifically, the fluid stratification aggregation characterization value is calculated according to the following formula:

[0090]

[0091] where M is the fluid stratification aggregation characterization value, k is the ratio of the number of abnormal interval segments inside the fluid to the number of wavelength interval segments, k0 is the preset reference value of the quantity ratio, R av is the average value of the curve coincidence degrees corresponding to each abnormal interval segment inside the fluid, R0 is the preset reference value of the average value of the curve coincidence degree, α is the preset weight coefficient of the quantity ratio, β is the preset weight coefficient of the curve coincidence degree, α + β = 1, and e is a constant.

[0092] Preferably, in the embodiment of the present invention, k = n1 / n2, where n1 is the number of abnormal interval segments inside the fluid, n2 is the number of wavelength interval segments, the value range of the preset reference value k0 of the quantity ratio is [0.15, 0.2], and the value range of the preset reference value R0 of the average value of the curve coincidence degree is [0.9R a , 0.95R a .

[0093] The weight coefficient α of the quantity ratio and the weight coefficient β of the curve coincidence degree are selected by those skilled in the art according to the ratio of the number of abnormal interval segments inside the fluid to the number of wavelength interval segments in the historical data and the influence degree of the curve coincidence degree on the calculation result. Preferably, the weight coefficient α of the quantity ratio can be set to 0.4, and the weight coefficient β of the curve coincidence degree can be set to 0.6.

[0094] Specifically, the present invention calculates the fluid stratification aggregation characterization value through the number of abnormal interval segments inside the fluid and the curve coincidence degree corresponding to each abnormal interval segment inside the fluid. During the actual comparison of the curve segment coincidence degree of the absorption spectrum curve of the collagen solution sample, the greater the number of wavelength interval segments where the curve segment coincidence degree does not meet the preset standard, the greater the difference degree between the upper collagen solution sample and the lower collagen solution sample. Similarly, the smaller the average value of the curve coincidence degrees corresponding to several abnormal interval segments inside the fluid, the greater the difference degree between the upper collagen solution sample and the lower collagen solution sample. Furthermore, the stratification difference degree of the collagen solution sample is intuitively quantified, the detection accuracy of the collagen solution with different hydrodynamic states is improved, and the interference of the stratification phenomenon of the active collagen solution caused by the difference in the hydrodynamic state inside on the purity detection of the collagen solution is excluded.

[0095] Specifically, please refer to Figure 4 shown, which is the logic flow chart for determining whether there is an obvious fluid stratification aggregation phenomenon in the embodiment of the present invention. The process of determining whether there is an obvious fluid stratification aggregation phenomenon in the collagen solution includes:

[0096] Compare the fluid stratification aggregation characterization value M with the preset fluid stratification aggregation characterization value threshold M0;

[0097] If the fluid stratification aggregation characterization value M is less than or equal to the fluid stratification aggregation characterization value threshold M0, it is determined that there is no obvious fluid stratification aggregation phenomenon in the collagen solution;

[0098] If the fluid stratification aggregation characterization value M is greater than the fluid stratification aggregation characterization value threshold M0, it is determined that there is an obvious fluid stratification aggregation phenomenon in the collagen solution.

[0099] Preferably, in the embodiment of the present invention, the value range of the fluid stratification aggregation characterization value threshold M0 is [2.9, 2.95].

[0100] Specifically, the process of obtaining the overall curve coincidence degree includes:

[0101] Collect the upper collagen solution sample and the lower collagen solution sample of the collagen solution after stirring for a predetermined time;

[0102] Use a visible spectrometer to measure the absorption spectra of the upper collagen solution sample and the lower collagen solution sample, and determine the absorption spectrum curve of the upper collagen solution sample and the absorption spectrum curve of the lower collagen solution sample;

[0103] Calculate the degree of overlap between the absorption spectral curve of the upper-layer collagen solution sample and the absorption spectral curve of the lower-layer collagen solution sample, and determine the degree of overlap as the overall curve overlap degree.

[0104] Specifically, the Euclidean distance between corresponding points on the two curves can be calculated through existing algorithms, and then the sum of all distances is obtained. The smaller the obtained value, the higher the overall curve overlap degree. The calculation of the overall curve overlap degree is a commonly used technical means in curve analysis and will not be elaborated here.

[0105] Specifically, the process of adjusting the ultrasonic power and ultrasonic duration during the ultrasonic extraction of the collagen solution includes:

[0106] Compare the overall curve overlap degree R’ with a preset reference value R of the overall curve overlap degree b ;

[0107] If the overall curve overlap degree R’ is greater than or equal to the reference value R of the overall curve overlap degree b , it is determined that no adjustment is made to the ultrasonic extraction of the collagen solution;

[0108] If the overall curve overlap degree R’ is less than the reference value R of the overall curve overlap degree b , the ultrasonic power and ultrasonic duration during the ultrasonic extraction of the collagen solution are adjusted;

[0109] Among them, the ultrasonic power has a negative correlation with the overall curve overlap degree, and the ultrasonic duration has a negative correlation with the overall curve overlap degree.

[0110] Preferably, in the embodiment of the present invention, the reference value R of the overall curve overlap degree b ranges from [90%, 93%]. The ultrasonic power and ultrasonic duration are parameters commonly used by those skilled in the art in ultrasonic-assisted extraction technology. Those skilled in the art can initially set the ultrasonic power according to the type of collagen solution to be extracted and make adjustments based on the initially set ultrasonic power. For example, in the extraction process of collagen solution from sheepskin, the initially set ultrasonic power can range from [210, 230], with the unit of the interval being W. Similarly, those skilled in the art can also initially set the ultrasonic duration according to the type of collagen solution to be extracted and make adjustments based on the initially set ultrasonic duration. For example, in the extraction process of collagen solution from sheepskin, the initially set ultrasonic duration ranges from [20, 30], with the unit of the interval being min.

[0111] Specifically, the present invention adjusts the ultrasonic extraction process of the collagen solution according to the overall curve coincidence degree of the absorption spectral curves of the upper-layer collagen solution sample and the lower-layer collagen solution sample after the collagen solution is stirred for a predetermined duration. Those skilled in the art can understand that if the stratification difference of the collagen solution sample is caused by different aggregation concentrations of protein molecules due to the difference in the hydrodynamic state of the collagen solution, by stirring the collagen solution, the stratification difference of the collagen solution sample can be significantly reduced, that is, the overall curve coincidence degree of the absorption spectral curves of the upper-layer collagen solution sample and the lower-layer collagen solution sample is relatively high, then the stratification and aggregation phenomenon of the collagen solution is not caused by impurities, and there is no need to adjust the ultrasonic extraction of the collagen solution. If the stratification difference of the collagen solution sample is caused by impurities in the collagen solution, by stirring the collagen solution, the stratification difference of the collagen solution sample cannot be significantly reduced, that is, the overall curve coincidence degree of the absorption spectral curves of the upper-layer collagen solution sample and the lower-layer collagen solution sample is relatively low. It is necessary to increase the ultrasonic power during the ultrasonic extraction process of the collagen solution and extend the ultrasonic duration during the ultrasonic extraction process of the collagen solution to improve the purity of the collagen solution during the extraction process. Furthermore, it is necessary to exclude the interference of the stratification phenomenon of the active collagen solution caused by the difference in the hydrodynamic state inside on the purity detection of the collagen solution, and effectively adjust the extraction process of the collagen solution according to the accurate result of the purity detection of the collagen solution.

[0112] So far, the technical solution of the present invention has been described in combination with the preferred embodiments shown in the drawings. However, those skilled in the art can easily understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

[0113] The above are only the preferred embodiments of the present invention and are not used to limit the present invention; for those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for improving the purity of active collagen using ultrasound-assisted extraction technology, characterized in that: include: Filling the collagen solution extracted by ultrasound into several packaging bottles, and judging whether the bubble release fluid space of the collagen solution is uniform based on the fluctuation degree of the liquid level of the collagen solution in the packaging bottles; In response to the unevenness of the bubble-released fluid space of the collagen liquid, sampling information of the collagen liquid after a preset standing time is obtained, wherein the sampling information includes an absorption spectrum curve of an upper layer collagen liquid sample and an absorption spectrum curve of a lower layer collagen liquid sample; Determine the curve segment overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in a plurality of wavelength intervals, and determine the wavelength interval where the curve segment corresponding to the curve segment overlap that does not meet the preset standard is located as an abnormal interval in the fluid; Calculating the fluid stratification and aggregation characterization value based on the number of abnormal intervals in the fluid and the overlap of curves corresponding to the abnormal intervals in each fluid, so as to determine whether the collagen liquid has obvious fluid stratification and aggregation phenomenon; The fluid stratification aggregation characterization value is calculated according to the following formula; Wherein, M is the fluid stratification aggregation characterization value, k is the ratio of the number of abnormal intervals in the fluid to the number of wavelength intervals, k0 is the preset reference value of the number ratio, R av is the average value of the curve coincidence corresponding to the abnormal interval segment inside each fluid, R0 is the preset reference value of the curve coincidence average value, α is the preset quantity ratio weight coefficient, β is the preset curve coincidence weight coefficient, and e is a constant; In response to the obvious fluid stratification and polymerization phenomenon of the collagen liquid, the overall overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after the collagen liquid is stirred for a predetermined time is obtained, and the ultrasonic power and the duration of the ultrasound during the ultrasonic extraction of the collagen liquid are adjusted according to the overall overlap of the curves; Comparing the overall curve coincidence with a preset reference value of the overall curve coincidence; If the overall coincidence of the curves is greater than or equal to the overall coincidence reference value of the curves, it is determined that no adjustment is made to the ultrasonic extraction of the collagen solution; If the overall overlap of the curves is less than the overall overlap reference value of the curves, adjusting the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution; Therein, the ultrasonic power is negatively correlated with the overall coincidence of the curve, and the ultrasonic duration is negatively correlated with the overall coincidence of the curve.

2. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 1, characterized in that: The process of determining the degree of fluctuation of the liquid level of the collagen solution in the packaging bottle is as follows: The liquid level heights of the collagen liquid in the several packaging bottles are obtained, and the standard deviation of the liquid level heights is calculated according to the liquid level heights of the collagen liquid in the several packaging bottles.

3. The method for improving the purity of active collagen by using ultrasound-assisted extraction technology according to claim 2, characterized in that: The process of determining whether the bubble-released fluid space of the collagen solution is uniform includes: Comparing the liquid level standard deviation with a preset liquid level standard deviation threshold; If the liquid level standard deviation is greater than the liquid level standard deviation threshold, it is determined that the bubble release fluid space of the collagen liquid is uneven.

4. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 3, characterized in that: The process of obtaining the sampling information includes: Collecting an upper layer collagen liquid sample and a lower layer collagen liquid sample of the collagen liquid after the preset standing time; Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample; The absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample are determined with the wavelength of light emitted by the visible spectrometer as the horizontal axis and the absorbance as the vertical axis.

5. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 4, characterized in that: The step of determining the curve segment overlap comprises: Dividing the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample into a plurality of wavelength intervals in the direction of the horizontal axis at the same preset wavelength interval; Determine the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample in each wavelength interval segment; The degree of overlap between the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample within the wavelength interval is calculated, and the degree of overlap is determined as the curve segment overlap degree.

6. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 5, characterized in that: The process of determining the abnormal interval section inside the fluid is as follows: The curve segment coincidence degree is compared with a preset curve segment coincidence degree reference value. If the curve segment coincidence degree is less than or equal to the curve segment coincidence degree reference value, it is determined that the wavelength interval segment where the curve segment corresponding to the curve segment coincidence degree is located is an abnormal interval segment inside the fluid.

7. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 1, characterized in that: The process of determining whether the collagen solution has obvious fluid stratification and aggregation phenomenon includes: Comparing the fluid stratified aggregation characterization value with a preset fluid stratified aggregation characterization value threshold; If the fluid stratification and aggregation characterization value is greater than the fluid stratification and aggregation characterization value threshold, it is determined that the collagen solution has obvious fluid stratification and aggregation phenomenon.

8. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 7, characterized in that: The process of obtaining the overall coincidence of the curves includes: collecting an upper collagen liquid sample and a lower collagen liquid sample of the collagen liquid after stirring for a predetermined time; Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample, and determine the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample; The degree of overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample is calculated, and the degree of overlap is determined as the overall overlap of the curves.

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