A device for accelerating cell resuscitation in batches

Abstract

The present invention relates to the technical field of cell resuscitation devices, and particularly relates to a device for batch accelerating cell resuscitation, which includes a mounting base. A resuscitation box body is arranged on the mounting base. A batch resuscitation mechanism for simultaneously performing water bath heating on a plurality of cryotube bodies is arranged inside the resuscitation box body. A water bath heating mechanism is arranged on the inner side wall of the resuscitation box body. An automatic feeding mechanism is arranged at the upper end of the batch resuscitation mechanism. A resuscitation auxiliary mechanism for disinfecting the outer surface of the cryotube is arranged at the upper end of the resuscitation box body. The automatic feeding mechanism includes a rectangular convex block movably mounted on a mounting disc. By setting the automatic feeding mechanism, through the mutual cooperation between the feeding funnel and the L-shaped pushing plate, the cryotube body is always in a horizontal state during the feeding process, and the liquid nitrogen in the externally rotated cryotube can automatically flow out before water bath heating, which can prevent the cryotube from exploding to a certain extent during the water bath heating process.

Description

Technical Field

[0001] The present invention relates to the technical field of cell recovery devices, and in particular to a device for batch accelerating cell recovery. Background Art

[0002] Cell recovery is a process of restoring cryopreserved in vitro cultures or bioactive materials to normal temperature at a certain rewarming rate. Whether it is microorganisms, animal cells, plant cells or in vitro cultured organs, they can all be cryopreserved using liquid nitrogen first and then recovered under appropriate temperature conditions.

[0003] However, the recovery devices in the prior art have a simple structure. Most of them are composed of a water bath heating component and a clamping component. The residual liquid nitrogen in the externally rotating cryotube cannot be discharged before water bath heating, and it is very easy to cause the explosion of the cryotube due to the excessive temperature difference inside and outside during heating. Moreover, the cryotube cannot be protected necessarily. If one of the cryotubes explodes due to the excessive temperature difference inside and outside during water bath heating, it is very easy to damage other cryotubes, resulting in unnecessary losses. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the present invention provides a device for batch accelerating cell recovery, which solves the technical problems that the recovery device in the prior art has a simple structure, the residual liquid nitrogen in the externally rotating cryotube cannot be discharged before water bath heating, and the cryotube is prone to explosion during heating, and has the advantages of being able to discharge liquid nitrogen before water bath heating and effectively preventing the explosion of the cryotube.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: A device for accelerating cell resuscitation in batches, including a mounting base, on which a resuscitation box body is provided. Inside the resuscitation box body, a cryopreservation tube body is placed. Inside the resuscitation box body, there is a batch resuscitation mechanism for performing water bath heating on multiple cryopreservation tube bodies simultaneously. On the inner side wall of the resuscitation box body, a water bath heating mechanism is provided. At the upper end of the batch resuscitation mechanism, an automatic feeding mechanism is provided. At the upper end of the resuscitation box body, a resuscitation auxiliary mechanism for disinfecting the outer surface of the cryopreservation tube is provided. During cell resuscitation, the cryopreservation tube body will enter the inside of the batch resuscitation mechanism under the action of the automatic feeding mechanism. Next, the batch resuscitation mechanism will transport the cryopreservation tube body into the inside of the water bath heating mechanism for heating and melting. The automatic feeding mechanism includes a rectangular convex block movably installed on a mounting disc. Inside the rectangular convex block, a pushing chute is opened. A feeding funnel is fixedly installed on the rectangular convex block. An L-shaped pushing plate is movably installed inside the pushing chute. A driving gear is movably installed on the rectangular convex block. After the cryopreservation tube body enters the inside of the pushing chute, the L-shaped pushing plate will horizontally push the cryopreservation tube body into the inside of the storage bracket. An electromagnet assembly is fixedly installed on the rectangular convex block. A tube body limiting rod is movably installed on the side of the feeding funnel. One end of the tube body limiting rod is located inside the feeding funnel, and the other end of the tube body limiting rod is located outside the feeding funnel and fixedly installed with a permanent magnet block. A return spring is sleeved outside the tube body limiting rod. The magnetic poles of the side of the permanent magnet block facing the electromagnet assembly are the same. When the electromagnet assembly is energized to generate magnetism, it will cause the tube body limiting rod to move towards the inside of the feeding funnel.

[0006] Preferably, the batch resuscitation mechanism includes an electric push rod fixedly installed inside the resuscitation box body. At the upper end of the electric push rod, a mounting disc is movably installed. On the mounting disc, there is a driving component for rotating the rectangular convex block. A number of rotating convex blocks are arranged at equal intervals along the circumferential direction on the mounting disc. On the rotating convex block, a storage bracket for placing the cryopreservation tube body is fixedly installed. An inner limiting rubber ring is fixedly installed inside the storage bracket. A fixed baffle is provided on the mounting disc. When there is a cryopreservation tube body on the storage bracket, the rotating convex block will rotate to a state parallel to the fixed baffle. After the staff takes out the cryopreservation tube body, the rotating convex block will rotate to a state perpendicular to the fixed baffle.

[0007] Preferably, inside the upper end of the electric push rod, there is a driving motor for driving the mounting disc to rotate reciprocally. When the driving motor is energized and operates, it will cause the mounting disc to rotate reciprocally at a certain angle.

[0008] Preferably, the water bath heating mechanism comprises a water bath box which can be detachably mounted inside the resuscitation box, a plurality of partition baffles are arranged at equal intervals inside the water bath box, independent water bath cavities are formed between adjacent partition baffles, circular through holes are penetrated through the partition baffles, a splash-proof shield is fixedly mounted on the outside of the storage bracket, the water bath box is in a circular shape, filled with clean water, the water level is aligned with the middle of the circular through hole, a heating component is provided at the bottom, and the clean water can be quickly heated according to the needs of the staff during use.

[0009] Preferably, the cross-sectional diameter of the splash shield decreases from top to bottom, and the lower end of the splash shield is sleeved on the outside of the storage bracket, which can effectively prevent a large amount of water droplets from splashing onto the tube mouth of the cryotube during the water bath process and causing contamination.

[0010] Preferably, a fixed convex strip is provided at the upper end of the L-shaped push plate, and an avoidance square groove matching the fixed convex strip is opened on the side of the rectangular convex block, and a touch switch is provided inside the avoidance square groove. When the fixed convex strip is located inside the avoidance square groove, the touch switch will be turned on, and when the fixed convex strip is moved out of the avoidance square groove, the touch switch will be automatically disconnected.

[0011] Preferably, the L-shaped pushing plate is meshed with a driving gear, and when the driving gear rotates, the L-shaped pushing plate can move horizontally along the pushing chute, thereby pushing the cryogenic tube outward.

[0012] Preferably, the touch switch is electrically connected to the electromagnet assembly via a wire. When the touch switch is turned on, the electromagnet assembly is energized to generate magnetism, and when the touch switch is turned off, the electromagnet assembly is non-magnetic.

[0013] Preferably, the resuscitation auxiliary mechanism includes a fixed bracket fixedly mounted on the resuscitation box, a movable disc is rotatably connected inside the fixed bracket, a rectangular through groove for avoiding the feeding funnel is provided on the movable disc, an annular enclosure is fixedly mounted on the fixed bracket, a square hole for spraying liquid is provided on the outer side of the annular enclosure, a liquid adding pipe is fixedly connected on the annular enclosure, when the feeding funnel moves up and down with the mounting disc, the movable disc will not interfere with the movement of the feeding funnel due to the presence of the rectangular through groove.

[0014] Preferably, a plurality of the liquid spraying square holes are provided at equal intervals, and a liquid adding pipe is connected to the ethanol storage tank. The alcohol solution in the ethanol storage tank will enter the interior of the annular enclosure through the liquid adding pipe and be sprayed out through the liquid spraying square holes.

[0015] By means of the above technical solution, the present invention provides a device for accelerating cell recovery in batches, which has at least the following beneficial effects:

[0016] 1. The present invention sets up an automatic feeding mechanism. By the mutual cooperation between the feeding funnel and the L-shaped pushing plate, the cryogenic storage tube body is always in a horizontal state during the feeding process, enabling the liquid nitrogen in the externally rotating cryogenic storage tube to flow out automatically before water bath heating, which can prevent the explosion of the cryogenic storage tube to a certain extent during the water bath heating process.

[0017] 2. The present invention sets up an automatic feeding mechanism. By the mutual cooperation between the rectangular convex block and the driving component, it can automatically place a plurality of cryogenic storage tube bodies into the interior of the storage bracket in sequence, which can effectively reduce the contact time between the staff and the cryogenic storage tube, improve the efficiency, and prevent the staff from being frostbitten by liquid nitrogen.

[0018] 3. The present invention sets up an automatic feeding mechanism. By the mutual cooperation between the electromagnet component and the tube body limiting rod, it can automatically press and limit the remaining cryogenic storage tubes during the horizontal pushing process, avoiding the premature dropping of the cryogenic storage tubes and causing movement interference to the horizontal movement of the L-shaped pushing plate.

[0019] 4. The present invention sets up a batch resuscitation mechanism. By the mutual cooperation between the mounting disc and several rotating convex blocks, it can perform water bath heating on a plurality of cryogenic storage tubes at one time, realizing batch processing and effectively improving the efficiency of cell resuscitation.

[0020] 5. The present invention sets up a batch resuscitation mechanism. By the mutual cooperation between the electric push rod and the mounting disc, it can automatically shake the cryogenic storage tubes reciprocally during water bath heating to ensure uniform heating. And after heating is completed, it will quickly remove the cryogenic storage tubes from the water, which can effectively avoid the increase in cell death rate caused by overheating.

[0021] 6. The present invention sets up a water bath heating mechanism. By the mutual cooperation between the partition baffle and the water bath tank, each cryogenic storage tube can be in an independent water bath cavity during water bath heating. In this way, even if a cryogenic storage tube explodes due to incomplete discharge of liquid nitrogen, it will not damage other cryogenic storage tubes.

[0022] 7. The present invention sets up a water bath heating mechanism. By the mutual cooperation between the splash-proof cover and the partition baffle, the partition baffle divides the annular water surface into multiple interconnected parts, which can effectively reduce the fluctuation of the water surface caused by shaking the cryogenic storage tubes. And the splash-proof cover can shield and protect the nozzle of the cryogenic storage tube, effectively preventing the water in the water bath tank from contaminating the nozzle of the cryogenic storage tube during the water bath process.

[0023] 8. The present invention sets up a resuscitation assistance mechanism. By the mutual cooperation between the annular enclosure and the liquid spraying square holes, it can automatically disinfect the outer surface of the cryogenic storage tube body after water bath heating, prevent the contamination of the operating table after the cryogenic storage tube is transferred to the sterile operating table, and also increase the success rate of cell resuscitation. Description of the Drawings

[0024] The accompanying drawings described herein are used to provide a further understanding of the present application and form a part of the present application. The schematic embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation of the present application. In the drawings:

[0025] Figure 1 is a three-dimensional view of the overall structure of the present invention;

[0026] Figure 2 is a schematic structural view of the interior of the recovery box body in the present invention;

[0027] Figure 3 is a schematic structural view of the batch recovery mechanism in the present invention;

[0028] Figure 4 is a schematic structural view of the automatic feeding mechanism in the present invention;

[0029] Figure 5 is a schematic structural view of the water bath heating mechanism in the present invention;

[0030] Figure 6 is a schematic structural view of the partition baffle in the present invention;

[0031] Figure 7 is a schematic structural view of the splash guard in the present invention;

[0032] Figure 8 is a schematic structural view of the L-shaped pusher plate in the present invention;

[0033] Figure 9 is a schematic structural view of the pusher chute in the present invention;

[0034] Figure 10 is a schematic structural view of the drive gear in the present invention;

[0035] Figure 11 is a schematic structural view of the tube body limiting rod in the present invention;

[0036] Figure 12 is a schematic structural view of the recovery assistance mechanism in the present invention.

[0037] In the figure: 1, mounting base; 2, recovery box body; 3, batch recovery mechanism; 301, electric push rod; 302, mounting disc; 303, rotating bump; 304, storage bracket; 305, limit rubber ring; 4, cryopreservation tube body; 5, water bath heating mechanism; 501, water bath box; 502, partition baffle; 503, water bath cavity; 504, circular through hole; 505, splash-proof cover; 6, automatic feeding mechanism; 601, rectangular bump; 602, feeding funnel; 603, driving assembly; 604, pushing chute; 605, L-shaped pushing plate; 606, driving gear; 607, electromagnet assembly; 608, tube body limiting rod; 609, permanent magnet block; 610, fixed rib; 7, recovery auxiliary mechanism; 701, fixed bracket; 702, movable disc; 703, rectangular through slot; 704, annular enclosing plate; 705, liquid spraying square hole; 706, liquid adding pipeline. Specific implementation manner

[0038] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0039] Embodiment 1

[0040] The recovery device in the prior art has a simple structure, and most of them are composed of a water bath heating component and a clamping component. Before water bath heating, the residual liquid nitrogen in the externally rotating cryopreservation tube cannot be discharged, and it is very easy for the cryopreservation tube to explode due to excessive internal and external temperature differences during heating. In order to solve this technical defect existing in the prior art, as Figures 1 - 4 and Figures 8 - 11 shown, this embodiment proposes a batch cell recovery acceleration device, which can automatically place a plurality of cryopreservation tube bodies 4 into the inside of the storage bracket 304 in sequence, which can effectively reduce the contact time between the staff and the cryopreservation tube, improve the efficiency, and prevent the staff from being frostbitten by liquid nitrogen. A recovery box body 2 is arranged on the mounting base 1 of the device, the cryopreservation tube body 4 is placed inside the recovery box body 2, a batch recovery mechanism 3 for water bath heating of a plurality of cryopreservation tube bodies 4 at the same time is arranged inside the recovery box body 2, a water bath heating mechanism 5 is arranged on the inner side wall of the recovery box body 2, an automatic feeding mechanism 6 is arranged at the upper end of the batch recovery mechanism 3, and a recovery auxiliary mechanism 7 for disinfecting the outer surface of the cryopreservation tube is arranged at the upper end of the recovery box body 2. During cell recovery, the cryopreservation tube body 4 will enter the inside of the batch recovery mechanism 3 under the action of the automatic feeding mechanism 6. Next, the batch recovery mechanism 3 will transport the cryopreservation tube body 4 into the inside of the water bath heating mechanism 5 for heating and melting.

[0041] In order to automatically discharge the residual liquid nitrogen in the cryotube before the water bath, an automatic feeding mechanism 6 is provided in this embodiment. Specifically, the automatic feeding mechanism 6 includes a rectangular convex block 601 movably installed on the mounting disc 302. A pushing chute 604 is provided inside the rectangular convex block 601. A feeding funnel 602 is fixedly installed on the rectangular convex block 601. An L-shaped pushing plate 605 is movably installed inside the pushing chute 604. A driving gear 606 is movably installed on the rectangular convex block 601. After the cryotube body 4 enters the inside of the pushing chute 604, the L-shaped pushing plate 605 will horizontally push the cryotube body 4 into the inside of the storage bracket 304. The L-shaped pushing plate 605 meshes with the driving gear 606. When the driving gear 606 rotates, it can make the L-shaped pushing plate 605 horizontally move along the pushing chute 604, thereby pushing the cryotube outwards. An electromagnet assembly 607 is fixedly installed on the rectangular convex block 601. A tube body limiting rod 608 is movably installed on the side of the feeding funnel 602. One end of the tube body limiting rod 608 is located inside the feeding funnel 602, and the other end of the tube body limiting rod 608 is located outside the feeding funnel 602 and is fixedly installed with a permanent magnet block 609. A return spring is sleeved outside the tube body limiting rod 608. The magnetic poles of the surface of the permanent magnet block 609 facing the electromagnet assembly 607 are the same. When the electromagnet assembly 607 is energized to generate magnetism, it will make the tube body limiting rod 608 move towards the inside of the feeding funnel 602. A fixed convex strip 610 is provided at the upper end of the L-shaped pushing plate 605. An avoidance square groove matching the fixed convex strip 610 is provided on the side of the rectangular convex block 601. A touch switch is provided inside the avoidance square groove. When the fixed convex strip 601 is located inside the avoidance square groove, the touch switch will be turned on. When the fixed convex strip 601 moves out of the inside of the avoidance square groove, the touch switch will automatically turn off. The touch switch is electrically connected to the electromagnet assembly 607 through a wire. When the touch switch is turned on, the electromagnet assembly 607 will be energized to generate magnetism. When the touch switch is turned off, the electromagnet assembly 607 has no magnetism.

[0042] According to the above content, during cell recovery processing, the staff will sequentially place a plurality of cryotubes into the inside of the feeding funnel 602. At this time, the cryotube placed first will fall into the inside of the pushing chute 604.

[0043] Next, as Figure 3 shown, the L-shaped pushing plate 605 will horizontally move along the pushing chute 604 under the action of the driving gear 606. When the L-shaped pushing plate 605 horizontally moves, it will push the cryotube into the inside of the limit rubber ring 305.

[0044] Moreover, during the movement of the L-shaped pusher plate 605, the fixed convex strip 610 will enter the inside of the avoidance square groove. At this time, the touch switch will be in the on state. Next, the electromagnet assembly 607 will generate magnetism to make the tube limiting rod 608 move towards the inside of the feeding funnel 602, so as to tightly limit the cryogenic tube above the pushing chute 604 and prevent the cryogenic tube above from interfering with the movement of the L-shaped pusher plate 605 during the pushing process.

[0045] After one-time pushing is completed, the L-shaped pusher plate 605 will move in the reverse direction under the action of the driving gear 606. When the fixed convex strip 610 completely moves out of the inside of the avoidance square groove, the electromagnet assembly 607 will lose magnetism. Next, the tube limiting rod 608 will move outwards under the action of the return spring, so as to release the limiting effect on the cryogenic tube. Subsequently, the cryogenic tube located above the pushing chute 604 will fall into the inside of the pushing chute 604.

[0046] Next, the rectangular convex block 601 will rotate a certain angle under the action of the driving assembly 603 to align the opening of the pushing chute 604 with the next storage bracket 304. Subsequently, the above-mentioned pushing process is repeated until all the cryogenic tubes inside the feeding funnel 602 are inserted into the corresponding storage brackets 304.

[0047] In this embodiment, by setting the automatic feeding mechanism 6 and using the mutual cooperation between the rectangular convex block 601 and the driving assembly 603, multiple cryogenic tube bodies 4 can be automatically placed into the storage brackets 304 in sequence, which can effectively reduce the contact time between the staff and the cryogenic tubes, improve the efficiency, and prevent the staff from being frostbitten by liquid nitrogen. Moreover, in this embodiment, by setting the automatic feeding mechanism 6 and using the mutual cooperation between the feeding funnel 602 and the L-shaped pusher plate 605, the cryogenic tube body 4 is always in a horizontal state during the feeding process, which can enable the liquid nitrogen in the externally rotated cryogenic tube to flow out automatically before water bath heating, and can prevent the cryogenic tube from exploding to a certain extent during the water bath heating process. In addition, in this embodiment, by setting the automatic feeding mechanism 6 and using the mutual cooperation between the electromagnet assembly 607 and the tube limiting rod 608, the remaining cryogenic tubes can be automatically tightly limited during the horizontal pushing process, avoiding the premature dropping of the cryogenic tubes and causing movement interference to the horizontal movement of the L-shaped pusher plate 605.

[0048] Embodiment Two

[0049] In order to improve the efficiency of cell resuscitation as much as possible and achieve batch processing, on the basis of Embodiment One, as Figures 1 - 4 and Figure 7As shown in the figure, in this embodiment, a batch resuscitation mechanism 3 is provided. Specifically, the batch resuscitation mechanism 3 includes an electric push rod 301 fixedly installed inside the resuscitation box body 2. The upper end of the electric push rod 301 is movably installed with an installation disc 302. Inside the upper end of the electric push rod 301, there is a driving motor for driving the installation disc 302 to rotate reciprocally. When the driving motor is energized and operates, the installation disc 302 will rotate reciprocally at a certain angle. On the installation disc 302, there is a driving assembly 603 for rotating the rectangular convex block 601. Along the circumferential direction of the installation disc 302, a number of rotating convex blocks 303 are arranged at equal intervals. On the rotating convex block 303, a storage bracket 304 for placing the cryopreservation tube body 4 is fixedly installed. Inside the storage bracket 304, a limiting rubber ring 305 is fixedly installed. On the installation disc 302, there is a fixed baffle. When there is a cryopreservation tube body 4 on the storage bracket 304, the rotating convex block 303 will rotate to a state parallel to the fixed baffle. After the staff takes out the cryopreservation tube body 4, the rotating convex block 303 will rotate to a state perpendicular to the fixed baffle.

[0050] According to the above content, as Figure 3 shown, initially, the rotating convex block 303 will be in a vertical state. Next, the cryopreservation tube body 4 will be horizontally inserted into the inside of the storage bracket 304 under the action of the automatic feeding mechanism 6. Subsequently, the rotating convex block 303 will automatically rotate to a state parallel to the fixed baffle.

[0051] Subsequently, the electric push rod 301 will automatically contract to move the installation disc 302 vertically downward. When the installation disc 302 moves downward, it will cause the cryopreservation tube body 4 to enter the inside of the water bath tank 501. At the same time, the installation disc 302 will reciprocally rotate (the reciprocating rotation angle is 25 degrees) under the action of the driving motor, so that the cryopreservation tube body 4 is in a reciprocating swing state to ensure uniform heating.

[0052] After water bath heating for a certain period of time, the installation disc 302 will move upward under the action of the electric push rod 301, so that the cryopreservation tube body 4 can be quickly removed from the water bath tank 501 to avoid an increase in cell death rate due to too long water bath time.

[0053] In this embodiment, by setting the batch resuscitation mechanism 3 and using the mutual cooperation between the installation disc 302 and a number of rotating convex blocks 303, multiple cryopreservation tubes can be subjected to water bath heating at one time, realizing batch processing, and effectively improving the efficiency of cell resuscitation. In addition, in this embodiment, by setting the batch resuscitation mechanism 3 and using the mutual cooperation between the electric push rod 301 and the installation disc 302, the cryopreservation tube can be automatically shaken reciprocally during water bath heating to ensure uniform heating. And after heating is completed, the cryopreservation tube will be quickly removed from the water, which can effectively avoid an increase in cell death rate due to overheating.

[0054] Embodiment Three

[0055] In order to prevent the cryotube from being contaminated during water bath heating, on the basis of the above embodiments, as Figure 2 well as Figures 5 - 7 shown, in this embodiment, a water bath heating mechanism 5 is provided. Specifically, the water bath heating mechanism 5 includes a water bath box 501 detachably installed inside the recovery box body 2. A number of partition baffles 502 are equidistantly arranged inside the water bath box 501. Independent water bath cavities 503 are formed between adjacent partition baffles 502. Circular through holes 504 are penetrated through the partition baffles 502. A splash-proof shield 505 is fixedly installed on the outside of the storage bracket 304. The water bath box 501 is annular, filled with clear water, the water surface height is aligned with the middle of the circular through holes 504, and a heating component is provided at the bottom, which can quickly heat the clear water according to the needs of the staff during use. The cross-sectional diameter of the splash-proof shield 505 decreases from top to bottom. The lower end of the splash-proof shield 505 is sleeved on the outside of the storage bracket 304, which can effectively prevent a large amount of water droplets from splashing onto the tube orifice of the cryotube during the water bath process and causing contamination.

[0056] According to the above content, during cell recovery processing, the cryotube body 4 will move into the interior of the water bath box 501 under the action of the electric push rod 301, and then, the cryotube body 4 will reciprocally shake following the installation disc 302.

[0057] As Figure 5 shown, after the cryotube enters the interior of the water bath box 501, each cryotube will be located inside a water bath cavity 503. Next, the cryotube will be evenly heated inside the water bath cavity 503 and quickly complete melting.

[0058] Moreover, as Figure 7 shown, a splash-proof shield 505 is sleeved on the outside of the storage bracket 304, which can effectively prevent the tube orifice of the cryotube from contacting water, thereby avoiding contamination at the tube orifice.

[0059] In this embodiment, by setting the water bath heating mechanism 5 and using the mutual cooperation between the partition baffle 502 and the water bath box 501, each cryotube can be separately located in an independent water bath cavity 503 during water bath heating. In this way, even if a cryotube explodes due to incomplete discharge of liquid nitrogen, it will not cause damage to other cryotubes; moreover, in this embodiment, by setting the water bath heating mechanism 5 and using the mutual cooperation between the splash-proof shield 505 and the partition baffle 502, the partition baffle 502 divides the annular water surface into multiple interconnected parts, which can effectively reduce the fluctuation of the water surface caused by shaking the cryotube. And the splash-proof shield 505 can shield and protect the tube orifice of the cryotube, which can effectively prevent the tube orifice of the cryotube from being contaminated by the water in the water bath box 501 during the water bath process.

[0060] Embodiment Four

[0061] In order to prevent the cryopreservation tube heated in a water bath from contaminating the sterile operating table, on the basis of the above-mentioned embodiment, a resuscitation auxiliary mechanism 7 is provided in this embodiment. Specifically, the resuscitation auxiliary mechanism 7 includes a fixed bracket 701 fixedly installed on the resuscitation box body 2. An activity disc 702 is rotatably connected inside the fixed bracket 701. A rectangular through groove 703 for avoiding the feeding funnel 602 is formed on the activity disc 702. An annular enclosing plate 704 is fixedly installed on the fixed bracket 701. Liquid spraying square holes 705 are formed on the outer side of the annular enclosing plate 704. A liquid adding pipeline 706 is fixedly connected to the annular enclosing plate 704. When the feeding funnel 602 moves up and down following the installation disc 302, due to the existence of the rectangular through groove 703, the activity disc 702 will not interfere with the movement of the feeding funnel 602. A plurality of liquid spraying square holes 705 are arranged at equal intervals. The liquid adding pipeline 706 is connected to an ethanol storage tank. The alcohol solution in the ethanol storage tank will enter the inside of the annular enclosing plate 704 through the liquid adding pipeline 706 and be sprayed out through the liquid spraying square holes 705.

[0062] According to the above content, after the water bath heating is completed, the installation disc 302 will move upward under the action of the electric push rod 301, so as to transfer the cryopreservation tube body 4 to the upper end of the resuscitation box body 2.

[0063] When the cryopreservation tube moves above the resuscitation box body 2, the ethanol solution (seventy percent ethanol) inside the liquid adding pipeline 706 will be sprayed out through a plurality of liquid spraying square holes 705, so as to automatically disinfect the outside of the cryopreservation tube.

[0064] In this embodiment, by setting the resuscitation auxiliary mechanism 7 and using the mutual cooperation between the annular enclosing plate 704 and the liquid spraying square holes 705, the outer surface of the cryopreservation tube body 4 can be automatically disinfected after the water bath heating, preventing the cryopreservation tube from contaminating the operating table after being transferred to the sterile operating table, and also increasing the success rate of cell resuscitation.

[0065] The control mode of the present invention is automatically controlled by a controller. The control circuit of the controller can be realized by simple programming by those skilled in the art. The provision of power also belongs to the common knowledge in this field. And the present invention is mainly used to protect mechanical devices, so the control mode and circuit connection of the present invention will not be explained in detail.

[0066] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0067] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A batch accelerated cell recovery device, comprising a mounting base (1), a recovery box (2) being arranged on the mounting base (1), a cryotube body (4) being placed inside the recovery box (2), characterized in that: The resuscitation box (2) is provided with a batch resuscitation mechanism (3) for simultaneously heating a plurality of cryotube bodies (4) in a water bath, the inner side wall of the resuscitation box (2) is provided with a water bath heating mechanism (5), the upper end of the batch resuscitation mechanism (3) is provided with an automatic feeding mechanism (6), and the upper end of the resuscitation box (2) is provided with a resuscitation auxiliary mechanism (7) for disinfecting the outer surface of the cryotube; The batch recovery mechanism (3) comprises an electric push rod (301) fixedly mounted inside the recovery box (2), a mounting disc (302) being movably mounted on the upper end of the electric push rod (301), an automatic feeding mechanism (6) comprising a rectangular protrusion (601) movably mounted on the mounting disc (302), a driving assembly (603) for rotating the rectangular protrusion (601) being provided on the mounting disc (302), a material pushing chute (604) being provided inside the rectangular protrusion (601), a material feeding funnel (602) being fixedly mounted on the rectangular protrusion (601), and a material pushing chute (604) being provided inside the rectangular protrusion (601). 04) is movably mounted with an L-shaped push plate (605), a driving gear (606) is movably mounted on the rectangular protrusion (601), an electromagnet assembly (607) is fixedly mounted on the rectangular protrusion (601), a tube body limiting rod (608) is movably mounted on the side of the feeding funnel (602), one end of the tube body limiting rod (608) is located inside the feeding funnel (602), the other end of the tube body limiting rod (608) is located outside the feeding funnel (602) and is fixedly mounted with a permanent magnet block (609), and a reset spring is sleeved on the outside of the tube body limiting rod (608); The mounting disc (302) is provided with a plurality of rotating protrusions (303) at equal intervals in the circumferential direction, a storage bracket (304) for placing the cryotube body (4) is fixedly mounted on the rotating protrusion (303), a limiting rubber ring (305) is fixedly mounted on the inner side of the storage bracket (304), and a fixed baffle is provided on the mounting disc (302); The L-shaped push plate (605) is meshed with the driving gear (606).

2. The device for accelerating cell recovery in batches according to claim 1, characterized in that: A driving motor for driving the mounting disc (302) to reciprocate is provided inside the upper end of the electric push rod (301).

3. The device for accelerating cell recovery in batches according to claim 1, characterized in that: The water bath heating mechanism (5) comprises a water bath box (501) which is detachably mounted inside the resuscitation box (2); a plurality of partition baffles (502) are arranged at equal intervals inside the water bath box (501); mutually independent water bath cavities (503) are formed between adjacent partition baffles (502); circular through holes (504) are formed through the partition baffles (502); and a splash shield (505) is fixedly mounted on the outer side of the storage bracket (304).

4. The device for accelerating cell recovery in batches according to claim 3, characterized in that: The cross-sectional diameter of the splash shield (505) decreases from top to bottom, and the lower end of the splash shield (505) is sleeved on the outside of the storage bracket (304).

5. The device for accelerating cell recovery in batches according to claim 1, characterized in that: The upper end of the L-shaped push plate (605) is provided with a fixed convex strip (610), and the side surface of the rectangular convex block (601) is provided with an avoidance square groove matching the fixed convex strip (610), and a touch switch is provided inside the avoidance square groove.

6. The device for accelerating cell recovery in batches according to claim 5, characterized in that: The touch switch is electrically connected to the electromagnet assembly (607) via a wire.

7. The device for accelerating cell recovery in batches according to claim 1, characterized in that: The resuscitation auxiliary mechanism (7) comprises a fixed bracket (701) fixedly mounted on the resuscitation box (2); a movable disc (702) is rotatably connected to the interior of the fixed bracket (701); a rectangular through slot (703) for avoiding the feeding funnel (602) is provided on the movable disc (702); an annular enclosure (704) is fixedly mounted on the fixed bracket (701); a liquid injection square hole (705) is provided on the outer side of the annular enclosure (704); and a liquid feeding pipe (706) is fixedly connected to the annular enclosure (704).

8. The device for accelerating cell recovery in batches according to claim 7, characterized in that: A plurality of liquid spraying square holes (705) are provided at equal intervals, and a liquid adding pipeline (706) is connected to an ethanol storage tank.

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