PCR eight-connection tube detection device and method

Abstract

The invention provides a PCR eight-connection tube detection device and method, the upper end of a light chopper is a conical table, two light shielding plates are arranged in the light chopper, and a detection blind area of the side wall of a PCR tube is in an open and exposed state in a cavity by utilizing the shaping effect of the conical table; a fan-shaped area formed by the two shading plates in the cavity enables one side of the PCR tube and an inner cavity of the connector to form a light path, a light path of the light source device penetrates through a local tube wall on one side of the PCR tube, enters the fan-shaped area and then acts on the sensing head through the inner cavity of the connector, so that the light transmission intensity of the local tube wall is received by the illumination intensity sensor, and the light intensity of the PCR tube is detected. The direction of the connecting pipe is twisted, so that local light-transmitting light rays of the pipe walls of all the positions of the PCR pipe sequentially enter the fan-shaped areas and are independently detected by the sensor, the wall thicknesses of all the positions of the PCR pipe and the uniformity of the wall thicknesses can be accurately detected, comprehensive wall thickness detection can be carried out on the specific connecting pipe structure of the PCR pipe body in the mode, the detection blind area is eliminated, the measurement range is widened, and the measurement precision is improved. And normal traceability, troubleshooting, optimization and adjustment of the PCR experiment are ensured.

Description

Technical Field

[0001] The invention belongs to medical device detection technology, and specifically relates to a PCR eight-tube detection device and method. Background Art

[0002] PCR, or polymerase chain reaction, is a detection technology for amplifying nucleic acids in vitro that was developed in the mid-1980s. PCR technology is a commonly used gene detection technology in molecular biology and is widely used in criminal investigation, paleontology, genetic diseases, prenatal diagnosis, organ transplantation, infectious pathogen detection, tumor gene detection and other fields. The working principle of PCR is that under high temperature conditions (95°C), the DNA double strands dissociate into single strands. When the temperature drops (60°C), the target DNA is quickly replicated under the joint action of DNA polymerase and specific primers. After multiple repeated cycles (generally 40 times), the target DNA is amplified to 100,000 or even 1 million times for target DNA observation and judgment. The thickness, uniformity, smoothness, and light transmittance of the PCR tube wall will affect the heat transfer and heat transfer speed, and thus affect the detection of PCR. The ultra-thin and uniform tube wall of PCR can achieve fast and accurate heat conduction, thereby achieving precise temperature control in the tube, so as to achieve the most suitable environmental temperature required for each stage of denaturation, complementation, and extension in the PCR experiment. It is an indispensable and important container device in PCR technology.

[0003] Since PCR experiments are sensitive to the ambient temperature of the reaction, the wall thickness of the PCR tube plays a decisive role in affecting the temperature of the reaction reagents in the PCR tube. Once the wall of the PCR tube is rough, the wall thickness is uneven, or the wall is too thick, it will affect the adhesion and uneven distribution of the reaction reagents in the PCR tube, and the temperature in the reaction tube will not reach the optimal temperature for the reagent amplification reaction, affecting the normal progress of the entire PCR expansion reaction. The existing technology controls the quality of the PCR tube wall, only ensuring the wall thickness accuracy and uniformity as much as possible at the process optimization level in the production stage, but has not produced targeted detection technology or detection equipment to ensure whether the wall thickness parameters, uniformity and smoothness of the tube wall meet the standards. Therefore, once there is a flaw in the production process of the PCR tube, causing tube wall errors and interfering with the experimental results of the PCR reaction during subsequent use, the lack of a mature wall thickness detection device makes it impossible to accurately trace and investigate the cause of the erroneous experimental results, making it impossible for the PCR experiment to be optimized and adjusted normally; in addition, conventional naked eye observation and caliper measurement methods have the defects of human observation errors, small measurement range, and low measurement accuracy, and are not suitable for the detection of PCR tube wall thickness; and because the PCR tube body is usually manufactured in the form of eight connected tubes, the wall thickness at the side wall between the connected tubes is blocked by the adjacent tube body and causes a detection blind area, so it is not easy to directly measure the detection blind area, so that conventional detection technology and detection devices are not applicable to the unique connected tube structure of the PCR tube body. Therefore, it is necessary to design a new detection device to solve the above problems. Summary of the invention

[0004] In response to the above-mentioned problems in the prior art, the present invention provides a PCR eight-tube detection device and method, which can effectively perform comprehensive wall thickness detection on the unique connecting tube structure of the PCR tube body, and effectively measure the wall thickness at the side walls between the connecting tubes, thereby eliminating the detection blind spots and improving the measurement range and accuracy, thereby ensuring the normal traceability, investigation and optimization adjustment of the PCR experiment.

[0005] The present invention is implemented by the following technical scheme: a PCR eight-tube detection device, comprising a light shield, wherein the light shield is a hollow container structure, the lower end of the light shield is closed, the upper end of the light shield is a conical table, the table surface of the conical table is provided with a mounting hole, the aperture of the mounting hole is adapted to the diameter of the cylindrical tube body in the PCR tube, and the inner cavity of the PCR tube is used to place a light source device;

[0006] A light-collecting hole is provided on the side wall of the sunshade, a connector is connected to the outside of the light-collecting hole, an inner cavity of the connector is communicated with the inner cavity of the sunshade through the light-collecting hole, a clamping hole is provided on the outer end of the connector, a sensor head is clamped in the clamping hole, and the clamping hole is sealed and connected to the sensor head, the sensor head is the sensing end of the light intensity sensor, and the output end of the light intensity sensor is connected to the display device;

[0007] The inner wall of the sunshade is provided with two vertically arranged sunshades, the inner ends of the two sunshades point to the axis of the sunshade, the outer ends of the two sunshades are connected to the inner wall of the sunshade and are distributed on both sides of the light hole, and the top view cross-section of the connection between the two sunshades and the inner wall of the sunshade presents a fan-shaped structure.

[0008] Furthermore, a limiting seat is spaced apart just above the mounting hole, the limiting seat is fixed by a bracket, a limiting hole is penetrated through the limiting seat body, a limiting nut is fixed on the limiting seat, a limiting bolt is threadedly fitted in the limiting nut, the limiting bolt is coaxial with the mounting hole, and the tail end of the limiting bolt is used to resist the PCR tube fitted in the mounting hole or the light source device in the tube.

[0009] Furthermore, a limiting sleeve is sleeved on the rear end of the limiting bolt, and a sliding piece made of metal is attached between the limiting sleeve and the rear end surface of the limiting bolt.

[0010] Furthermore, the inner cavity of the shading device is provided with an inclined light guide plate, the difference between the inclination angle of the light guide plate and the inclination angle of the inclined wall of the conical platform is ≤15°, the side edges of the light guide plate are respectively sealed with the two shading plate bodies, and the bottom edge of the light guide plate is sealed with the lower edge of the light-collecting hole; the inner wall of the conical platform facing the light guide plate and the plate surface of the light guide plate facing the sensor head are both provided with a reflective coating.

[0011] Furthermore, the inclination of the light guide plate is equal to the inclination of the inclined wall of the conical platform, and the reflective coating material is a reflective heat-insulating coating.

[0012] Furthermore, when the PCR tube link composed of the plurality of PCR tubes is in the maximum bending state, the side wall of any PCR tube forms an angle β with the adjacent side wall of the PCR tube separated by one PCR tube, and the cone angle α of the cone stage and the angle β meet the condition that α≤β.

[0013] Furthermore, the distance a between the axis of the shading device and the inner end surface of the shading plate, and the radius b of the cylindrical tube body in the PCR tube meet the following conditions: 0≤ab≤1mm.

[0014] Furthermore, a filling plate is provided between the inner end surfaces of the two shading plates, and the upper end surface of the filling plate is attached to or maintains a gap with the bottom of the PCR tube.

[0015] Furthermore, a layer of expanded polytetrafluoroethylene sealing tape is adhered to one or more surfaces of the inner wall of the mounting hole, the inner end surface of the shading plate, and the upper end surface of the filling plate.

[0016] Furthermore, the shading plate, the plate surface of the filling plate facing the sensor head, and the inner wall of the connector are all provided with a reflective coating.

[0017] Furthermore, the shade is supported on the base plate by a plurality of pillars, and the light source device is an LED lamp bead.

[0018] The present invention also provides a PCR eight-tube detection method, comprising the following steps:

[0019] S1: Select any PCR tube in the PCR tube chain as the PCR tube to be tested, place a light source device in the tube of the PCR tube to be tested, insert the PCR tube to be tested into the mounting hole on the top of the light shield, so that the PCR tubes adjacent to the PCR tube to be tested on the left and right are squeezed into an inclined state by the inclined surface of the conical stage, and the tube walls on both sides of the PCR tube to be tested enter the inner cavity of the light shield in an open state;

[0020] S2: Turn on the light source device in the PCR tube to be tested. The light source device outputs a transmitted light that passes through the side wall of the PCR tube to be tested, passes through the inner cavity of the connector and is received by the sensor head. The light intensity sensor then feeds back the light intensity parameter to the display device for display and recording.

[0021] S3: Twist the PCR tube to make it rotate around the axis of the PCR tube to be tested, so that the side walls of the PCR tube to be tested successively enter the fan-shaped area clamped by the two shading plates, and make the local transmitted light of the tube wall successively enter the fan-shaped area and be collected by the sensor head. The reading and display device displays the change state of the light intensity. The change value A of the upper and lower limits of the light intensity is set as the critical value, and the following judgment measures are taken according to the critical value:

[0022] When the light intensity change value displayed by the display device is ≤ the critical value A, the wall thickness of each part of the PCR tube to be tested meets the uniformity requirement;

[0023] When the light intensity change value displayed by the display device is greater than the critical value A, the wall thickness uniformity of the PCR tube to be tested does not meet the requirements and is discarded.

[0024] Furthermore, the critical value A=75 Lux.

[0025] The beneficial effects of the present invention are as follows: by utilizing the shaping effect of the conical platform structure at the top of the shading device and the bendability of the PCR connecting tube, after any PCR tube in the PCR connecting tube is inserted into the clamping hole of the conical platform, the tube body of the adjacent connecting tube is squeezed into a curved shape by the inclined wall of the conical platform and adheres to the inclined wall of the conical platform, so that the tube body of the PCR tube entering the clamping hole is alone in the optically closed cavity inside the shading device, and the detection blind area of ​​its side wall is in an open and exposed state in the cavity; then, in the fan-shaped area formed by the two shading plates in the cavity, one side of the PCR tube forms a light path with the inner cavity of the connector alone, and at this time, the light source device in the PCR tube is turned on, and the light path passes through the local area on one side of the PCR tube. The tube wall enters the fan-shaped area guided by the shading plate, and then acts on the sensor head through the inner cavity of the connector, so that the light transmittance of the local tube wall is received by the light intensity sensor. At this time, by twisting the direction of the connecting tube, the local light transmittance of the tube wall of the PCR tube can enter the fan-shaped area one after another and be detected separately by the sensor, and then the numerical changes of the reading and measurement display device can be accurately detected, so as to accurately detect the wall thickness and uniformity of each part of the PCR tube. This method can perform comprehensive wall thickness detection on the unique connecting tube structure of the PCR tube body, and effectively measure the wall thickness at the side wall between the connecting tubes, eliminate the detection blind area and improve the measurement range and accuracy, so as to ensure the normal traceability, investigation and optimization adjustment of the PCR experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of an embodiment of the present invention;

[0027] Figure 2 A partial side view of an embodiment of the present invention;

[0028] Figure 3 A partial top view of an embodiment of the present invention;

[0029] Figure 4 A schematic diagram of the three-dimensional structure of a light shield in one embodiment of the present invention;

[0030] Figure 5 This is a schematic diagram of the bending state of the PCR tube in one embodiment of the present invention;

[0031] Figure 6 FIG. 1 is a schematic diagram of a light path trajectory of a light source device in an embodiment of the present invention.

[0032] In the figure: 10-shade, 10a-lighting hole, 10b-reflective coating, 11-conical platform, 11a-mounting hole, 12-connector, 12a-clamping hole, 13-shading plate, 14-light guide plate, 15-filling plate, 16-expanded polytetrafluoroethylene sealing tape, 20-light intensity sensor, 21-sensing head, 30-limiting seat, 30a-limiting hole, 31-bracket, 32-limiting nut, 33-limiting bolt, 34-limiting sleeve, 40-bottom plate, 41-pillar, 5-PCR connecting tube, 50-PCR tube, 51-light source device, 52-wire. DETAILED DESCRIPTION

[0033] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0034] like Figure 1-Figure 4 As shown, a PCR eight-tube detection device includes a light shield 10, wherein the light shield 10 is a hollow container structure, and the lower end of the light shield 10 is closed, so as to provide an independent optical detection environment in the inner cavity of the light shield 10 to eliminate the interference of external ambient light; the upper end of the light shield 10 is a conical platform 11, and the table surface of the conical platform 11 is provided with a mounting hole 11a, and the aperture of the mounting hole 11a is adapted to the diameter of the cylindrical tube body in the PCR tube 50; because the connecting ribs between the PCR tubes 5 have a certain elastic bending performance, the PCR tubes 5 as a whole can be bent to form an arc array body (such as Figure 5 As shown in the figure, when any PCR tube 50 in the PCR tube link 5 is inserted into the mounting hole 11a, the inclined wall of the conical platform 11 can guide the adjacent PCR tube 50 to form an inclined posture, so that the PCR tube link 5 is bent to a certain extent, so that the detection blind area at the side wall of the PCR tube 50 is exposed, which is conducive to the subsequent comprehensive detection work;

[0035] A light-collecting hole 10a is provided on the side wall of the sunshade 10. A connector 12 is connected to the outside of the light-collecting hole 10a. The inner cavity of the connector 12 is connected to the inner cavity of the sunshade 10 through the light-collecting hole 10a. A clamping hole 12a is provided on the outer end of the connector 12. A sensor head 21 is clamped in the clamping hole 12a to form a sealed connection. The sensor head 21 is the sensing end of the light intensity sensor 20. The output end of the light intensity sensor 20 is connected to a display device. When the light source in the PCR tube 50 outputs light and passes through the wall of the PCR tube 50, the light intensity is attenuated. The corresponding light intensity is generated by the thickness parameter of the wall of the PCR tube 50 and output to the sensor head 21 through the inner cavity of the connector 12. It is collected by the light intensity sensor 20, and then the corresponding illumination parameter is displayed by the display device for the inspection personnel to read and measure, so as to judge whether the wall thickness parameter corresponding to the illumination parameter meets the standard.

[0036] The inner wall of the shade 10 is provided with two vertically arranged shading plates 13, the inner ends of the two shading plates 13 are directed to the axis of the shade 10, the outer ends of the two shading plates 13 are connected to the inner wall of the shade 10, and are distributed on both sides of the light-collecting hole 10a. The top view of the two shading plates 13 connected to the inner wall of the shade 10 is a fan-shaped structure (such as Figure 3 Therefore, only the light in the sector area surrounded by the shading plate 13 will be detected by the sensor head 21, so that the PCR tube 50 is on the left side of the sector area (direction according to Figure 1-Figure 3 ) The transmitted light of the local tube wall is smoothly transmitted to the sensor head 21, while the light transmitted by the remaining part of the tube wall of the PCR tube 50 outside the fan-shaped area is blocked by the shading plate 13, thereby eliminating the interference of the transmitted light of the remaining tube wall, so that the sensor head 21 can specifically collect the local transmitted light of the PCR tube 50, thereby achieving a targeted detection effect of the local wall thickness.

[0037] In this embodiment, a limiting seat 30 is spaced apart just above the mounting hole 11a. The limiting seat 30 is fixed by a bracket 31. A limiting hole 30a is penetrated through the limiting seat 30. A limiting nut 32 is fixed on the limiting seat 30. A limiting bolt 33 is sleeved in the limiting nut 32 in a threaded manner. The limiting bolt 33 is coaxial with the mounting hole 11a. The tail end of the limiting bolt 33 is used to resist the PCR tube 50 sleeved in the mounting hole 11a and the light source device 51 in the tube. Since the bending state of the PCR connecting tube 5 has a certain resilience, the PCR tube 5 is prevented from bending due to the resisting effect of the limiting bolt 33 in the upper limiting seat 30. 0 is firmly embedded in the closed cavity of the light shield 10, and at the same time, the adjacent PCR tube 50 can be stably attached to the surface of the conical table 11, which is beneficial to the subsequent detection work; at the same time, a light source device 51 for light transmission detection is placed in the PCR tube 50, and its power supply wire needs to be led out from the tube mouth of the PCR tube 50 to the outside. When the tube mouth of the PCR tube 50 is pressed by the limiting bolt 33, the limiting bolt 33 also presses the wire of the light source device 51, so as to prevent the displacement of the light source device 51 caused by the traction of the wire, ensure that the light source device 51 is always in the PCR tube 50, and further facilitate the smooth progress of the light transmission detection work.

[0038] In this embodiment, a limiting sleeve 34 is sleeved on the rear end of the limiting bolt 33, and a metal slide is attached between the limiting sleeve 34 and the rear end surface of the limiting bolt 33. When the inspector rotates the PCR tube 5 to scan the tube wall, the PCR tube 50 is in a rotating state, and the upper limiting sleeve 34 is promptly driven to rotate synchronously. The setting of the internal slide can prevent the limiting bolt 33 from loosening during rotation, ensuring that the PCR tube 5 can be continuously pre-tightened, attached and fixed by the limiting bolt 33 in the rotating state.

[0039] In this embodiment, the inner cavity of the light shield 10 is provided with an inclined light guide plate 14, the difference between the inclination angle of the light guide plate 14 and the inclination angle of the inclined wall of the conical platform 11 is ≤15°, the two side edges of the light guide plate 14 are respectively sealed with the two light shielding plates 13, and the bottom edge of the light guide plate 14 is sealed with the lower edge of the light hole 10a; the inner wall of the conical platform 11 facing the light guide plate 14 and the plate surface of the light guide plate 14 facing the sensor head 21 are both provided with a reflective coating 10b;

[0040] Since the setting of the inclined wall of the conical platform 11 can expose the detection blind area of ​​the side wall of the PCR tube 50, but the inclined wall of the conical platform 11 itself will become an obstacle to the light transmitted from the side wall of the PCR tube 50, by setting the light guide plate 14 and the reflective structure under the conical platform 11, the transmitted light from the side wall of the PCR tube 50 can be successfully introduced into the sensor head 21; therefore, when the lateral transmitted light passing through the side wall of the PCR tube 50 is emitted to the inner wall of the conical platform 11, it is reflected by the reflective coating 10b to the light guide plate 14 below. Since the design inclination angle of the light guide plate 14 is close to the inclined wall of the conical platform 11, the light can be emitted into the inner cavity of the connector 12 again in a direction close to the lateral direction, and successfully enter the sensor head 21 to be collected and detected.

[0041] Furthermore, due to the structural arrangement of the inner wall of the conical table 11 and the light guide plate 14, the transmitted light beam can smoothly reach the sensor head 21 after only two reflections, thereby minimizing the number of reflections and reducing the energy loss caused by multiple reflections. At the same time, the reflective coating 10b design of the inner wall of the conical table 11 and the light guide plate 14 can further ensure sufficient light beam reflectivity and eliminate the loss interference caused by the light beam being converted into heat, so that the intensity of the terminal light beam entering the sensor head 21 is as close as possible to the original transmitted light beam that penetrates the side wall of the PCR tube 50, thereby ensuring that the detection parameters of the sensor head 21 can fully restore the actual state of the PCR tube 50 and ensure the accuracy and authenticity of the detection results.

[0042] In this embodiment, the slope of the light guide plate 14 is equal to the slope of the inclined wall of the conical table 11, so that the initial transmitted light beam continues to maintain the original angle to enter the sensor head 21 after secondary reflection through the inclined wall of the conical table 11 and the light guide plate 14, further reducing the probability of multiple reflections and ensuring that most of the transmitted light beam can successfully enter the sensor head 21.

[0043] In this embodiment, the reflective coating 10b is made of reflective thermal insulation paint. The characteristics of the reflective thermal insulation paint are used to further ensure the light reflection efficiency and reduce the degree of light-to-heat conversion, so that the light reflected by the inclined wall of the conical table 11 and the light guide plate 14 is as close to the original light intensity as possible, thereby ensuring the accuracy and authenticity of the test results.

[0044] In this embodiment, if Figure 5As shown, when the PCR tube link 5 composed of a plurality of PCR tubes 50 is in the maximum bending state, the side wall of any PCR tube 50 forms an angle β with the adjacent side wall of the PCR tube 50 separated by one PCR tube 50, and the cone angle α of the conical platform 11 and the angle β meet the condition that α≤β, thereby ensuring that the cone angle of the conical platform 11 does not exceed the bending limit angle of the PCR tube link 5, preventing the PCR tube link 5 from being damaged by excessive twisting, and allowing the PCR tube link 5 to be attached to the inclined wall of the conical platform 11 at a reasonable bending angle.

[0045] Preferably, since the PCR tube link 5 is shipped in the form of eight tube links, its bending limit is the circular array form of eight tube bodies. Therefore, the angle between the axes of two adjacent PCR tubes 50 is 360° / 8=45°, wherein the angle between the axis of any tube body and the tube body of the PCR tube 50 one tube apart is 90°. Since the cylindrical tube wall of the PCR tube 50 is flush with its axis, it can be seen that the limit value of the angle β is 90°. Therefore, the cone angle α can be taken as 90°, thereby ensuring that the PCR tube link 5 can be fully bent within the allowable degree, so that the blind area of ​​the side wall of the PCR tube 50 to be tested is fully expanded and exposed.

[0046] In the present embodiment, the distance a between the axis of the light shielding device 10 and the inner end surface of the light shielding plate 13 and the radius b of the cylindrical tube body in the PCR tube 50 meet the following conditions: 0≤ab≤1mm, which can ensure that the gap between the PCR tube 50 and the light shielding plate 13 is small enough to prevent excessive light leakage from the gap from interfering with the detection result; preferably, in the present embodiment, ab=0, so that the distance between the axis of the light shielding device 10 and the inner end surface of the light shielding plate 13 is exactly equal to the radius of the PCR tube 50, so that the tube wall of the PCR tube 50 is attached to the inner end surface of the light shielding plate 13 (at this time, the process ensures the smoothness of the inner end surface of the light shielding plate 13, which can prevent the PCR tube 50 from being worn), and in this attached state, the light leakage interference at the gap of the light shielding plate 13 is completely eliminated. At the same time, since the lower end of the PCR tube 50 is a conical end, the light shielding plate 13 is provided with an inclined surface matching the conical end, so that the end surface of the light shielding plate 13 is closely attached to the side wall of the PCR tube 50, ensuring the complete sealing of the fan-shaped area where the local side wall of the PCR tube 50 is located, thereby obtaining accurate detection results.

[0047] In this embodiment, a filling plate 15 is provided between the inner end surfaces of the two shading plates 13 to ensure that no light leakage occurs in the gap between the shading plates 13; the upper end surface of the filling plate 15 is attached to the bottom of the PCR tube 50 to further ensure that no light leakage occurs between the filling plate 15 and the bottom of the PCR tube 50 (at this time, the process ensures the smoothness of the upper end surface of the filling plate 15, which can prevent the PCR tube 50 from being worn), thereby further ensuring the complete sealing of the fan-shaped area where the local side wall of the PCR tube 50 is located, thereby obtaining accurate detection results.

[0048] Preferably, in this embodiment or other embodiments, a layer of expanded polytetrafluoroethylene sealing tape 16 may be adhered to the inner wall of the mounting hole 11a, the inner end surface of the light shielding plate 13 and the upper end surface of the filling plate 15. The expanded polytetrafluoroethylene sealing tape 16 has the advantages of flexibility, compressibility and lubricity. When the PCR tube 50 to be tested is inserted into the mounting hole 11a of the light shielding device 10 for detection, the elastic compression of the expanded polytetrafluoroethylene sealing tape 16 on the inner wall of the mounting hole 11a, the inner end surface of the light shielding plate 13 and the upper end surface of the filling plate 15 can make it closely adhere to the outer wall surface of the PCR tube 50 to be tested, further ensuring the optical sealing of the sector area, preventing light leakage interference and improving the detection accuracy; at the same time, when the PCR tube 50 to be tested is rotated to perform scanning detection of each local side wall, the lubricating contact of the expanded polytetrafluoroethylene sealing tape 16 can effectively reduce the friction loss of the outer wall surface of the PCR tube 50 to be tested, ensure that the physical state of the outer wall surface of the PCR tube 50 to be tested is not affected, and further ensure the optical detection accuracy.

[0049] In this embodiment, the shading plate 13, the plate surface of the filling plate 15 facing the sensor head 21, and the inner wall of the connector 12 are all provided with a reflective coating 10b. Since the light is transmitted through the microstructure of the wall of the PCR tube 50, a small amount of scattered light in different directions will be generated. The light-to-heat energy conversion caused by the scattered light entering the side walls at various locations will cause the uncontrollability of the light intensity in the inner cavity. The reflective coating 10b coated on the shading plate 13, the filling plate 15 and the inner wall of the connector 12 makes most of the scattered light reflected by the reflective coating 10b, further reducing the degree of light-to-heat conversion in the inner cavity space and the uncontrollability of the light intensity, and finally being received by the sensor head 21, thereby ensuring the controllability of the optical detection object and the accuracy of the detection result.

[0050] In this embodiment, the shade 10 is supported on the base plate 40 by a plurality of pillars 41, so that after the light intensity sensor 20 is installed on one side of the shade 10, the base plate 40 can maintain overall balance to prevent tipping over; the light source device 51 is an LED lamp bead, and the small size of the LED lamp bead can facilitate complete loading into the narrow space inside the PCR tube 50.

[0051] This embodiment also provides a PCR eight-tube detection method, comprising the following steps:

[0052] S1: Select any PCR tube 50 in the PCR tube link 5 as the tube body to be tested, place an LED light source device 51 in the tube body to be tested, and pull the LED wire out of the tube body, insert the tube body to be tested into the mounting hole 11a at the top of the light shield 10, so that the inclined surfaces of the conical platform 11 of the PCR tube 50 wall adjacent to the left and right of the tube body to be tested are squeezed into an inclined state. At this time, the connecting ribs between the tubes are in a state of reboundable bending deformation, and the upper limiting bolt 33 is screwed in, so that the limiting sleeve 34 is against the tube mouth of the PCR tube 50 to be tested, so that the PCR tube 50 to be tested is stably fixed in the mounting hole 11a, and the bending state of the PCR tube link 5 is maintained, forming a state as shown in FIG. Figure 1 In the state shown, at this time, the tube walls of the PCR tube 50 to be tested are in an open state and enter the inner cavity of the light shielding device 10, so as to facilitate the optical detection work;

[0053] S2: Figure 6 As shown, the power supply assembly connected by the wire turns on the LED light source device 51 in the PCR tube 50. Due to the shaping effect of the inclined wall of the conical platform 11 on the PCR tube 5, the blind areas on both sides of the PCR tube 50 to be tested are not blocked. A large amount of transverse transmission light output by the optical device passes through the side wall of the PCR tube 50 and is emitted to the inclined wall of the conical platform 11. The reflective coating 10b on the inner side of the inclined wall reflects the transverse transmission light to form a vertical reflection light facing downward, which is received by the light guide plate 14 set at the same inclination angle below, and is reflected by the reflective coating 10b on the surface of the light guide plate 14 to form a transverse reflection light toward the left side. After passing through the inner cavity of the connector 12, it is received by the sensor head 21, and the light intensity sensor 20 feeds back the light intensity parameter to the display device for display, which is recorded by the inspected personnel. At this time, the measured light intensity is 210Lux.

[0054] S3: The tester holds the PCR tube 5 and twists it, so that the PCR tube 5 rotates around the axis of the PCR tube 50 to be tested (direction as shown in FIG. Figure 6 As shown by the curved arrows), the side walls of the PCR tube 50 to be tested successively enter the fan-shaped area clamped by the two light shielding plates 13 (as shown in FIG. Figure 3 As shown), the light source device 51 is kept in the emitting state so that the local transmitted light of each tube wall successively enters the fan-shaped area and is collected by the sensor head 21, and the torsion angle is ensured to reach 360° to ensure that each tube wall can be subjected to comprehensive light transmission detection; at this time, due to the error in the wall thickness of the PCR tube 50, the intensity of the transmitted light changes, and the detection personnel read the change state of the light intensity displayed on the display device, and the upper and lower limits of the reading value are 170Lux~240Lux, and the change value is 70Lux, which means that the wall thickness of each part of the PCR tube 50 meets the uniformity requirement, and the PCR tube 50 is disassembled and another PCR tube 50 is placed in the installation hole 11a, and the above detection work is repeated;

[0055] Since the variation value of 70 Lux is the measured value of most standard PCR tubes 50, a small margin is set as the critical value, i.e., 75 Lux. In other embodiments, when the light intensity variation value displayed by the display device is greater than 75 Lux, it means that the manufacturing error of the wall thickness of each part of the PCR tube 50 is too large, causing the floating value of the intensity of the transmitted light to be too large. It can be determined that the uniformity of the wall thickness of the PCR tube 50 does not meet the requirements, and the PCR tube 5 is discarded.

[0056] By checking the PCR tube 5 in the above manner, the wall thickness and uniformity of each part of the PCR tube 50 can be accurately detected. This method can perform a comprehensive wall thickness detection on the unique tube structure of the PCR tube 50 body, and effectively measure the wall thickness at the side wall between the tubes, eliminating the detection blind spot and improving the measurement range and accuracy, ensuring the normal traceability, troubleshooting and optimization adjustment of the PCR experiment.

[0057] The above description is only a preferred embodiment of the present invention and does not constitute a formal limitation on the present invention. It should be understood that for ordinary technicians in this field, the embodiments may be equivalently replaced by other forms, which should be included in the protection scope of the present invention within the scope of the features defined in the claims.

Claims

1. A PCR eight-tube detection device, comprising a light shield, characterized in that: The light shield is a hollow container structure, the lower end of the light shield is closed, the upper end of the light shield is a conical table, the table top of the conical table is provided with a mounting hole, the aperture of the mounting hole is adapted to the diameter of the cylindrical tube body in the PCR tube, and the inner cavity of the PCR tube is used to place the light source device; A light-collecting hole is provided on the side wall of the sunshade, a connector is connected to the outside of the light-collecting hole, an inner cavity of the connector is communicated with the inner cavity of the sunshade through the light-collecting hole, a clamping hole is provided on the outer end of the connector, a sensor head is clamped in the clamping hole, and the clamping hole is sealed and connected to the sensor head, the sensor head is the sensing end of the light intensity sensor, and the output end of the light intensity sensor is connected to the display device; The inner wall of the sunshade is provided with two vertically arranged sunshades, the inner ends of the two sunshades point to the axis of the sunshade, the outer ends of the two sunshades are connected to the inner wall of the sunshade and are distributed on both sides of the light hole, and the top view cross-section of the connection between the two sunshades and the inner wall of the sunshade presents a fan-shaped structure.

2. The PCR eight-tube detection device according to claim 1, characterized in that: A limiting seat is spaced apart just above the mounting hole, the limiting seat is fixed by a bracket, a limiting hole is penetrated through the seat body of the limiting seat, a limiting nut is fixed on the limiting seat, a limiting bolt is threadedly fitted in the limiting nut, the limiting bolt is coaxial with the mounting hole, and the tail end of the limiting bolt is used to resist the PCR tube fitted in the mounting hole or the light source device inside the PCR tube.

3. The PCR eight-tube detection device according to claim 1, characterized in that: The inner cavity of the sunshade is provided with an inclined light guide plate, the difference between the inclination angle of the light guide plate and the inclination angle of the inclined wall of the conical platform is ≤15°, the two side edges of the light guide plate are respectively sealed with the two sunshades, and the bottom edge of the light guide plate is sealed with the lower edge of the light-collecting hole; the inner wall of the conical platform facing the light guide plate and the plate surface of the light guide plate facing the sensor head are both provided with a reflective coating.

4. The PCR eight-tube detection device according to claim 1, characterized in that: When the PCR tube link composed of a plurality of the PCR tubes is in the maximum bending state, the side wall of any PCR tube forms an angle β with the side wall of a PCR tube arranged one PCR tube apart, and the cone angle α of the cone stage and the angle β meet the condition that α≤β.

5. The PCR eight-tube detection device according to claim 1, characterized in that: The distance a between the axis of the light shield and the inner end surface of the light shielding plate and the radius b of the cylindrical tube body in the PCR tube meet the following conditions: 0≤ab≤1mm.

6. The PCR eight-tube detection device according to claim 1, characterized in that: A filling plate is connected between the inner end surfaces of the two shading plates, and the upper end surface of the filling plate is attached to or maintains a gap with the bottom of the PCR tube.

7. The PCR eight-tube detection device according to claim 6, characterized in that: The plate surface of the light shielding plate facing the sensor head and the plate surface of the filling plate facing the sensor head are both provided with a reflective coating.

8. The PCR eight-tube detection device according to claim 1, characterized in that: The shade is supported on the bottom plate by a plurality of pillars, the light source device is an LED lamp bead, and the inner wall of the connector is provided with a reflective coating.

9. A PCR eight-tube detection method, using the PCR eight-tube detection device according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: Select any PCR tube in the PCR tube chain as the PCR tube to be tested, place a light source device in the tube of the PCR tube to be tested, insert the PCR tube to be tested into the mounting hole on the top of the light shield, so that the PCR tubes adjacent to the PCR tube to be tested on the left and right are squeezed into an inclined state by the inclined surface of the conical stage, and the tube walls on both sides of the PCR tube to be tested enter the inner cavity of the light shield in an open state; S2: Turn on the light source device in the PCR tube to be tested. The light source device outputs a transmitted light that passes through the side wall of the PCR tube to be tested, passes through the inner cavity of the connector and is received by the sensor head. The light intensity sensor then feeds back the light intensity parameter to the display device for display and recording. S3: Twist the PCR tube to make it rotate around the axis of the PCR tube to be tested, so that the side walls of the PCR tube to be tested successively enter the fan-shaped area clamped by the two shading plates, and make the local transmitted light of the tube wall successively enter the fan-shaped area and be collected by the sensor head. The reading and display device displays the change state of the light intensity. The change value A of the upper and lower limits of the light intensity is set as the critical value, and the following judgment measures are taken according to the critical value: When the light intensity change value displayed by the display device is ≤ the critical value A, the wall thickness of each part of the PCR tube to be tested meets the uniformity requirement; When the light intensity change value displayed by the display device is greater than the critical value A, the wall thickness uniformity of the PCR tube to be tested does not meet the requirements and is discarded.

10. The PCR eight-tube detection method according to claim 9, characterized in that: The critical value A=75 Lux.

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