A quantitative detection device for fluorescent immunochromatography

Abstract

The present invention relates to the technical field of immunochromatography detection, and discloses a quantitative detection device for fluorescence immunochromatography. The device is designed to include a sample loading module, a fluorescence detection module, a temperature control module and a conveying module. The sample loading module, the fluorescence detection module, the temperature control module and the conveying module are all arranged in a chassis. The conveying module includes a first conveying part, which is a linear conveying part. The temperature control module includes a copper tube, and a straight heat exchange part is arranged on the copper tube. The sample loading module, the fluorescence detection module, the temperature control module and the conveying module cooperate with each other to quickly control the detection sample to the temperature required for the experiment, which can effectively reduce the interference of temperature on the quantitative detection of fluorescence immunochromatography, ensure the accuracy of the detection result, and improve the detection precision. At the same time, the high degree of automation of the entire equipment can further reduce the difficulty of operation.

Description

Technical Field

[0001] The invention relates to the technical field of immunochromatographic detection, and in particular to a quantitative detection device for fluorescent immunochromatography. Background Art

[0002] Oxidized low-density lipoprotein (oxLDL) is the product of low-density lipoprotein (LDL) after oxidation in the body, and is closely related to the development of atherosclerosis and cardiovascular diseases. Oxidized low-density lipoprotein can be quantitatively detected by fluorescent immunochromatography. During quantitative analysis, the operator drops the blood sample onto the reagent card, and after a certain interval, the reagent card is placed in the analytical instrument, and the instrument reads the quantitative results. This method has the characteristics of high sensitivity, high specificity, rapidity and simplicity, and is suitable for clinical diagnosis and biomedical research.

[0003] However, existing fluorescent immunochromatography quantitative detection equipment is easily affected by temperature and light. External light will directly interfere with the analysis results of the analytical instrument when the analytical instrument analyzes fluorescence, and temperature will not only cause changes in the conformation and spectral properties of fluorescent molecules, but different temperatures will also cause non-specific binding to varying degrees, especially at room temperature close to the human body. Under this temperature environment, the activity of the protein is high, and both specific and non-specific binding are likely to occur, which may seriously affect the analysis results. Especially in some weak positive tests, the influence of temperature can easily lead to misjudgment of the test results. Summary of the invention

[0004] The present invention aims to provide a quantitative detection device for fluorescent immunochromatography to solve the problem that the existing fluorescent immunochromatography equipment is easily disturbed, thereby affecting the detection results.

[0005] The present invention is achieved through the following technical solutions:

[0006] A quantitative detection device for fluorescent immunochromatography comprises a sample loading module, a fluorescence detection module, a temperature control module and a conveying module arranged in a chassis, characterized in that the conveying module comprises a first conveying part, on which a plurality of sample heat exchange components are fixed, the temperature control module comprises a copper tube, on which a straight heat exchange part is arranged, one side of the conveyor belt in the first conveying part is placed between two of the heat exchange parts, and the heat exchange part is parallel to the conveying direction of the first conveying part, the conveying module also comprises a second conveying part, and the second conveying part comprises a lifting area and a horizontal lateral movement area, the top of the lifting area is a reagent card sample dropping area, and the operation of dropping the sample onto the reagent card is completed at the reagent card sample dropping area, the horizontal lateral movement area is provided with a fluorescence detection area, and a fluorescence detection module is provided above the fluorescence detection area.

[0007] In a possible design, it includes a sample heat exchange component, which includes a first heat exchange plate, a second heat exchange plate and a disposable heat exchange tube. The first heat exchange plate and the second heat exchange plate are detachably fixed, and a portion of the disposable heat exchange tube is bent and placed between the first heat exchange plate and the second heat exchange plate.

[0008] In a possible design, a sample storage cup is fixed to one end of the disposable heat exchange tube, the interior of the sample storage cup is connected to the interior of the disposable heat exchange tube, a placement seat is fixed to the first heat exchange plate, the placement seat is used to place the sample storage cup, the other end of the disposable heat exchange tube vertically downward passes through the second heat exchange plate, when the sample storage cup is placed on the placement seat, the maximum level of the sample dripped into the sample storage cup does not exceed the highest position of the bend of the disposable heat exchange tube.

[0009] In a possible design, a fixing plate is fixed on the sample storage cup, and a lifting control mechanism is provided in the chassis. After the lifting control mechanism contacts the fixing plate, the sample storage cup can be lifted by lifting the fixing plate.

[0010] In a possible design, the first conveying part includes a conveyor belt, and the conveyor belt is controlled by a driving motor.

[0011] In a possible design, the first conveying part includes two identical conveyor belts, both of which are fixed with a first mounting buckle, and each of the sample heat exchange components is fixed with two second mounting buckles, the second mounting buckle at the upper end of the same sample heat exchange component is fixed to the first mounting buckle on the upper conveyor belt, and the second mounting buckle at the lower end is fixed to the first mounting buckle on the lower conveyor belt, and the two first mounting buckles fixed to the same sample heat exchange component are located in the same vertical position, a center hole is opened at the center of the first mounting buckle, a first magnet is fixed in the center hole, the second mounting buckle is provided with a center protrusion that cooperates with the center hole, and a second magnet is fixed on the center protrusion.

[0012] In a possible design, the second conveying part includes a reagent card placement slot and a conveyor belt group arranged on both sides of the reagent card placement slot, the conveyor belt group is symmetrically arranged with the reagent card placement slot as the center, each of the conveyor belt groups includes a conveyor belt and a plurality of pulleys, and the plurality of pulleys are connected and driven by one conveyor belt.

[0013] In a possible design, two connecting columns are fixed at both ends of the reagent card placement slot, and each end of the reagent card placement slot is fixedly connected to the conveyor belt through the connecting columns. The reagent card placement slot is provided with an observation window and a card insertion port on one side. The observation window is used for observing the C / T line of the reagent card, and the card insertion port is used for inserting the reagent card into the reagent card placement slot.

[0014] In a possible design, temperature control plates are fixed at both the reagent card sample dripping place and the fluorescence detection place. When the conveyor belt transports the reagent card placement slot to the temperature control plate, the temperature control plate can keep the reagent card placement slot and the reagent card fixed on the reagent card placement slot in a horizontal state. In the chassis, a discarded reagent card temporary storage area is provided below the card insertion window. The discarded reagent card temporary storage area is used to store discarded reagent cards after use. When the reagent card placement slot on the conveyor belt moves from a position close to the discarded reagent card temporary storage area to a position of the card insertion window, the card insertion port of the reagent card placement slot faces downward. When the reagent card placement slot runs along a route from the card insertion window-reagent card sample dripping place-fluorescence detection place-discarded reagent card temporary storage area, the card insertion port of the reagent card placement slot is only oriented toward the horizontal sides and the vertical upward direction.

[0015] In a possible design, a drip port is provided on the top of the chassis, a cover is provided at the drip port, the cover is hinged to the chassis at one point, a sealing ring is fixed on the cover, and a switch is also fixed at the hinge position between the cover and the chassis. When the cover is open, the switch is in a pop-up state, and when the cover is closed, the switch is in a pressed state.

[0016] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0017] 1. The present invention can quickly control the test sample to the temperature required for the experiment through the cooperation between the sample addition module, the fluorescence detection module, the temperature control module and the conveying module, which can effectively reduce the interference of temperature on the quantitative detection of fluorescence immunochromatography, ensure the accuracy of the test results, and improve the detection accuracy. At the same time, the high degree of automation of the entire equipment can further reduce the difficulty of operation.

[0018] 2. The chassis of the present invention can fully seal the sample adding module, fluorescence detection module, temperature control module and conveying module, which can not only keep the detection environment in the chassis warm, but also prevent external light sources from entering the chassis to interfere with the measurement of the fluorescence detection module. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings described herein are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation of the embodiments of the present invention. In the drawings:

[0020] Figure 1 It is a structural schematic diagram of the present invention;

[0021] Figure 2 is a side view of the present invention;

[0022] Figure 3 For the present invention Figure 2 Sectional view at AA in the middle;

[0023] Figure 4 For the present invention Figure 3 The enlarged view of point B in the middle;

[0024] Figure 5 For the present invention Figure 3 Enlarged view of point C in the middle;

[0025] Figure 6 This is a schematic diagram of the connection between the reagent card placement slot and the conveyor belt in an embodiment of the present invention;

[0026] Figure 7 A half-section view of a sample heat exchange component in an embodiment of the present invention;

[0027] Figure 8 A top view of a sample storage cup according to an embodiment of the present invention;

[0028] Fig. 9 Schematic diagram of the connection component between the conveyor belt and the sample heat exchange component in an embodiment of the present invention.

[0029] The reference numerals represent: 1-chassis, 2-operation panel, 3-cover plate, 4-copper tube, 5-reagent card placement slot, 501-connecting column, 502-observation window, 503-card insertion port, 6-conveyor belt, 7-pulley, 8-temporary storage area for discarded reagent cards, 9-fluorescence detection module, 10-sample heat exchange assembly, 101-first heat exchange plate, 1011-placement seat, 102-second heat exchange plate, 11-conveyor belt, 12-lifting control mechanism, 13-temperature control plate, 14-switch, 15-disposable heat exchange tube, 16-sample storage cup, 1601-fixed plate, 17-window sealing cover, 18-suction magnet, 19-trapezoidal block, 20-first mounting buckle, 2001-first magnet, 21-second mounting buckle, 2101-second magnet. DETAILED DESCRIPTION

[0030] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments and drawings. The exemplary embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.

[0031] Examples, such as Figures 1 to 9As shown, this embodiment includes a sample loading module, a fluorescence detection module 9, a temperature control module and a conveying module, wherein the sample loading module, the fluorescence detection module 9, the temperature control module and the conveying module are all arranged in a chassis 1, the conveying module includes a first conveying part, a plurality of sample heat exchange components 10 are fixed on the first conveying part, the first conveying part is a linear conveying, the temperature control module includes a copper tube 4, a straight heat exchange part is provided on the copper tube 4, one side of the conveyor belt 11 in the first conveying part is placed between the two heat exchange parts, when the sample heat exchange component 10 fixed on the first conveying part moves to this side, the temperature of the copper tube 4 will quickly cause the temperature of the sample heat exchange component 10 to change, and the heat exchange part is parallel to the conveying direction of the first conveying part.

[0032] The conveying module also includes a second conveying part, which includes a lifting area and a horizontal lateral movement area. The top of the lifting area is a reagent card sample dropping area, and the operation of dropping the sample onto the reagent card is completed at the reagent card sample dropping area. A fluorescence detection area is provided on the horizontal lateral movement area, and a fluorescence detection module 9 is provided above the fluorescence detection area. After the reagent card completes the sample dropping at the reagent card sample dropping area, it waits for a reaction time. After the reaction is completed, the reagent card is transported to the fluorescence detection area through the second conveying part, and then the detection is completed through the fluorescence detection module 9.

[0033] It should be noted that the fluorescence detection module 9 preferably uses a fluorescence reader to read the fluorescence intensity of the test line and the control line and calculate the T / C value. The content of specific substances in the sample is calculated through the built-in standard curve of the instrument to ensure the accuracy of the test results.

[0034] Furthermore, the sample heat exchange assembly 10 includes a first heat exchange plate 101, a second heat exchange plate 102 and a disposable heat exchange tube 15. The first heat exchange plate 101 and the second heat exchange plate 102 are detachably fixed to each other. A portion of the disposable heat exchange tube 15 is placed between the first heat exchange plate 101 and the second heat exchange plate 102 in a bent state. A sample storage cup 16 is fixed to one end of the disposable heat exchange tube 15. The interior of the sample storage cup 16 is communicated with the interior of the disposable heat exchange tube 15. A placement seat 1011 is fixed to the first heat exchange plate 101. The placement seat 1011 is used to place the sample storage cup 16. The other end of the disposable heat exchange tube 15 passes vertically downward through the second heat exchange plate 102. When the sample storage cup 16 is placed on the placement seat 1011, the maximum level of the sample liquid dripped into the sample storage cup 16 does not exceed the highest position of the bending point of the disposable heat exchange tube 15 At this time, even if the sample storage cup 16 is full of sample liquid, the sample liquid will not flow out from the other end of the disposable heat exchange tube 15 to the reagent card. After the sample is dripped into the sample storage cup 16, the sample storage cup 16 is lifted so that the height of the sample storage cup 16 is higher than the highest position of the bending part of the disposable heat exchange tube 15, so that the sample liquid in the sample storage cup 16 can drip onto the sample card from the other end of the disposable heat exchange tube 15. Among them, the disposable heat exchange tube 15 is made into a tube with a small diameter and a long length, which can increase the heat exchange area between the disposable heat exchange tube 15 and the heat exchange plate. The diameter of the disposable heat exchange tube 15 is less than 1 mm. When the sample liquid flows from the sample storage cup 16 through the bending part of the disposable heat exchange tube 15 to the other end, the sample liquid can quickly exchange heat with the copper tube 4, the first heat exchange plate 101 and the second heat exchange plate 102, and the disposable heat exchange tube 15 reaches the temperature required for the detection reaction.

[0035] Furthermore, a fixing plate 1601 is fixed on the sample storage cup 16, and a lifting control mechanism 12 is provided in the chassis 1. The lifting control mechanism 12 includes a claw with a U-shaped notch. After the claw contacts the fixing plate 1601, the sample storage cup 16 can be lifted by lifting the fixing plate 1601, thereby realizing automatic sample dripping. When the claw is lifted to a level higher than the sample heat exchange component 10, the lifting control mechanism 12 as a whole will not interfere with the operation of the first conveying part.

[0036] At the same time, the first conveying part includes the conveyor belt 11, which is controlled by a driving motor. A plurality of sample heat exchange components 10 can be fixed on the conveyor belt 11, and the temperature treatment of a plurality of samples can be completed quickly and continuously through the plurality of sample heat exchange components 10. When the first conveying part transports the test sample, the heat exchange part can quickly heat the sample heat exchange component 10 to the required test temperature, thereby further ensuring that the sample liquid is quickly heated to the temperature required by the experiment before reacting with the reagent card, so as to ensure the experimental temperature and reduce the occurrence of non-specific binding during detection.

[0037] Furthermore, the first conveying part includes two identical conveyor belts 11, and the two conveyor belts 11 are fixed with a first mounting buckle 20, and each of the sample heat exchange components 10 is fixed with two second mounting buckles 21, the second mounting buckle 21 at the upper end of the same sample heat exchange component 10 is fixed to the first mounting buckle 20 on the upper conveyor belt 11, and the second mounting buckle 21 at the lower end is fixed to the first mounting buckle 20 on the lower conveyor belt 11, and the two first mounting buckles 20 fixed to the same sample heat exchange component 10 are located in the same vertical position, a center hole is opened at the center of the first mounting buckle 20, and a first magnet 2001 is fixed in the center hole, and a center protrusion cooperating with the center hole is provided on the second mounting buckle 21, and a second magnet 2101 is fixed on the center protrusion, and the fixation between the first mounting buckle 20 and the second mounting buckle 21 is achieved by the mutual attraction between the first magnet 2001 and the second magnet 2101.

[0038] In this embodiment, the second conveying part includes a reagent card placement slot 5 and a conveyor belt 6 group arranged on both sides of the reagent card placement slot 5, the conveyor belt 6 group is symmetrically arranged with the reagent card placement slot 5 as the center, each of the conveyor belt 6 groups includes a conveyor belt 6 and a plurality of pulleys 7, and the plurality of pulleys 7 are connected and driven by one conveyor belt 6, two connecting columns 501 are fixed at both ends of the reagent card placement slot 5, and each end of the reagent card placement slot 5 is fixedly connected to the conveyor belt 6 through the connecting column 501, and a reagent card storage slot is provided on the reagent card placement slot 5. The reagent card is fixed to the reagent card placement slot 5 by placing the reagent card in the reagent card storage slot, and the reagent card is driven by the conveyor belt 6 to complete the movement of different positions, thereby automatically completing the dripping of the reagent card and the fluorescence measurement after the dripping of the sample, with a high degree of automation. At the same time, the reaction time on the reagent card after the dripping of the sample can be stably and accurately controlled, and the time variable can be effectively controlled.

[0039] Advantageously, a temperature control plate 13 is fixed at both the reagent card sample dropping location and the fluorescence detection location. When the conveyor belt 6 transports the reagent card placement slot 5 to the temperature control plate 13, the temperature control plate 13 can keep the reagent card placement slot 5 and the reagent card fixed on the reagent card placement slot 5 in a horizontal state. At the same time, a temperature control tube is also provided in the temperature control plate 13, and the temperature control tube can further keep the reagent card at the temperature control plate 13 at the required test temperature.

[0040] At present, many detection devices that use immunochromatography to achieve quantitative detection mostly have only a single reagent card placement slot 5. Moreover, the reagent card placement slot is not convenient to use. The main reason is that the reagent card loading and unloading operation is troublesome. For this reason, the present embodiment further designs the reagent card placement slot 5. Figure 5 and Figure 6 The reagent card placement slot 5 is provided with an observation window 502 and a card insertion port 503 on one side. The observation window 502 is used for observing the C / T line of the reagent card, and the card insertion port 503 is used for inserting the reagent card into the reagent card placement slot 5. On the side of the chassis 1, a card insertion window is provided near the reagent card sample drop location. When the conveyor belt 6 drives the reagent card placement slot 5 to move to the card insertion window, the card insertion port 503 on the reagent card placement slot 5 is horizontally oriented toward the card insertion window. At this time, the C / T line of the reagent card can be inserted upwards into the reagent card placement slot 5 from the card insertion window. In the chassis 1, a discarded reagent card temporary storage area 8 is provided below the card insertion window. The discarded reagent card temporary storage area 8 is used for For storing used discarded reagent cards, when the reagent card placement slot 5 on the conveyor belt 6 moves from a position near the discarded reagent card temporary storage area 8 to a card insertion window position, the card insertion port 503 of the reagent card placement slot 5 faces downward, and the used reagent cards in the reagent card placement slot 5 are automatically transferred to the discarded reagent card temporary storage area 8. It is only necessary to regularly clean the discarded reagent card temporary storage area 8. When the reagent card placement slot 5 runs along the route from the card insertion window-reagent card sample dropping area-fluorescence detection area-discarded reagent card temporary storage area 8, the card insertion port 503 of the reagent card placement slot 5 only has directions toward the horizontal sides and the vertical upward direction, and the reagent card will not fall out of the reagent card placement slot 5, which greatly facilitates the card insertion operation, and only the reagent card needs to be inserted.

[0041] At the same time, the main temperature control positions of the copper tube 4 in this embodiment are located at the heat exchange part, the reagent card sample dropping place and the fluorescence detection place. The energy consumption during temperature control is small, and the heat exchange part and the sample heat exchange component 10 are coordinated to change the temperature environment very quickly, and the temperature variables during detection can be changed quickly and efficiently.

[0042] Advantageously, sealing plate assemblies are provided at the card insertion window and the waste reagent card temporary storage area 8, and the sealing plate assembly includes a window sealing cover 17, the upper end of which is hinged on the chassis 1, and a closing magnet 18 is fixed to the lower end of the window sealing cover 17. The chassis 1 is also fixed with a closing magnet 18. When the window sealing cover 17 is closed, the two closing magnets 18 attract each other to close the window sealing cover 17 more tightly. A trapezoidal block 19 is also fixed above the hinged position of the window sealing cover 17. The trapezoidal block 19 limits the window sealing cover 17 to prevent the window sealing cover 17 from rotating to a vertical state, thereby ensuring that when the operator lets go, the window sealing cover 17 can rotate downward by itself to close the window.

[0043] It should be noted that low temperature can reduce nonspecific binding during the detection process. The temperature range is 4-10°C. In this environment, the binding between antigen and antibody is more stable, which helps to form more antigen-antibody complexes. This is especially important for detecting weak positive samples. It can also reduce nonspecific binding. Therefore, the main function of copper tube 4 is to quickly cool down.

[0044] In this embodiment, a sample dripping port is provided on the top of the chassis 1, and a cover plate 3 is provided at the sample dripping port. The cover plate 3 is hinged on the chassis 1 at one point, and a sealing ring is fixed on the cover plate 3. The sealing ring cooperates with the cover plate 3 to make the cover plate 3 and the sample dripping port fit tightly, thereby completely isolating the chassis 1 and preventing the inside of the chassis 1 from being disturbed by external light sources. At the same time, the cover plate 3 can also play a role in keeping the inside of the chassis 1 warm.

[0045] Furthermore, a switch 14 is fixedly provided at the hinge position between the cover 3 and the chassis 1. When the cover 3 is opened, the switch 14 is in a pop-up state, and when the cover 3 is closed, the switch 14 is in a pressed state. The opening and closing state of the switch 14 can be used to determine whether the cover 3 is open or closed. At the same time, the opening and closing of the switch 14 can also be used to determine whether the fluorescence detection module 9 can perform detection, thereby ensuring that the detection environment of the fluorescence detection module 9 is isolated from external light sources and ensuring the accuracy of the detection results.

[0046] Furthermore, this embodiment also includes a data processing system, which converts the fluorescence signal into a digital signal, and the data processing system calculates the concentration value.

[0047] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A quantitative detection device for fluorescence immunochromatography, comprising a sample loading module, a fluorescence detection module (9), a temperature control module and a transport module arranged in a chassis (1), characterized in that: The conveying module comprises a first conveying part, on which a plurality of sample heat exchange components (10) are fixed, the temperature control module comprises a copper tube (4), on which a straight heat exchange part is provided, one side of a conveyor belt (11) in the first conveying part is placed between two of the heat exchange parts, and the heat exchange part is parallel to the conveying direction of the first conveying part; The transport module further comprises a second transport portion, the second transport portion comprising a lifting area and a horizontal lateral movement area, the top of the lifting area being a reagent card sample dropping area, where the operation of dropping the sample onto the reagent card is completed, a fluorescence detection area being provided on the horizontal lateral movement area, and a fluorescence detection module (9) being provided above the fluorescence detection area; The invention also comprises a sample heat exchange assembly (10), wherein the sample heat exchange assembly (10) comprises a first heat exchange plate (101), a second heat exchange plate (102) and a disposable heat exchange tube (15), wherein the first heat exchange plate (101) and the second heat exchange plate (102) are detachably fixed to each other, and a portion of the disposable heat exchange tube (15) is placed between the first heat exchange plate (101) and the second heat exchange plate (102) in a bent state; A sample storage cup (16) is fixed to one end of the disposable heat exchange tube (15), the interior of the sample storage cup (16) is in communication with the interior of the disposable heat exchange tube (15), a placement seat (1011) is fixed to the first heat exchange plate (101), the placement seat (1011) is used to place the sample storage cup (16), the other end of the disposable heat exchange tube (15) vertically passes through the second heat exchange plate (102) downward, when the sample storage cup (16) is placed on the placement seat (1011), the highest level of the sample liquid dripped into the sample storage cup (16) does not exceed the highest position of the bend of the disposable heat exchange tube (15); A fixing plate (1601) is fixedly provided on the sample storage cup (16), and a lifting control mechanism (12) is provided in the chassis (1). After the lifting control mechanism (12) contacts the fixing plate (1601), the sample storage cup (16) can be lifted by lifting the fixing plate (1601).

2. A quantitative detection device for fluorescent immunochromatography according to claim 1, characterized in that: The first conveying part comprises a conveyor belt (11), and the conveyor belt (11) is controlled by a driving motor.

3. A quantitative detection device for fluorescent immunochromatography according to claim 2, characterized in that: The first conveying part comprises two identical conveyor belts (11), and the two conveyor belts (11) are both fixed with a first mounting buckle (20). Two second mounting buckles (21) are fixed to each sample heat exchange component (10), and the second mounting buckle (21) at the upper end of the same sample heat exchange component (10) is fixed to the first mounting buckle (20) on the upper conveyor belt (11), and the second mounting buckle (21) at the lower end is fixed to the first mounting buckle (20) on the lower conveyor belt (11), and the two first mounting buckles (20) fixed to the same sample heat exchange component (10) are located in the same vertical position, a center hole is opened at the center of the first mounting buckle (20), and a first magnet (2001) is fixed in the center hole, and a center protrusion that cooperates with the center hole is provided on the second mounting buckle (21), and a second magnet (2101) is fixed on the center protrusion.

4. The quantitative detection device of fluorescent immunochromatography according to claim 1, characterized in that: The second conveying part comprises a reagent card placement slot (5) and a conveyor belt (6) group arranged on both sides of the reagent card placement slot (5), the conveyor belt (6) group being symmetrically arranged with the reagent card placement slot (5) as the center, each of the conveyor belt (6) groups comprising a conveyor belt (6) and a plurality of pulleys (7), and the plurality of pulleys (7) are connected and driven by one conveyor belt (6).

5. A quantitative detection device for fluorescent immunochromatography according to claim 4, characterized in that: Two connecting posts (501) are fixed at both ends of the reagent card placement slot (5), and each end of the reagent card placement slot (5) is fixedly connected to the conveyor belt (6) via the connecting posts (501). The reagent card placement slot (5) is provided with an observation window (502) and a card insertion port (503) provided on one side. The observation window (502) is used for observing the C and T lines of the reagent card, and the card insertion port (503) is used for inserting the reagent card into the reagent card placement slot (5).

6. A quantitative detection device for fluorescent immunochromatography according to claim 5, characterized in that: A temperature control plate (13) is fixed at both the reagent card sample dropping position and the fluorescence detection position. When the conveyor belt (6) transports the reagent card placement slot (5) to the temperature control plate (13), the temperature control plate (13) can keep the reagent card placement slot (5) and the reagent card fixed on the reagent card placement slot (5) in a horizontal state. In the case (1), a waste reagent card temporary storage area (8) is provided below the card insertion window. The waste reagent card temporary storage area (8) is used to store the waste reagent cards after use. When the reagent card placement slot (5) on the conveyor belt (6) moves from a position close to the discarded reagent card temporary storage area (8) to a card insertion window position, the card insertion port (503) of the reagent card placement slot (5) faces downwards. When the reagent card placement slot (5) moves along a route from the card insertion window - the reagent card sample drop location - the fluorescence detection location - the discarded reagent card temporary storage area (8), the card insertion port (503) of the reagent card placement slot (5) only has two directions, one facing horizontally and one facing vertically upwards.

7. The quantitative detection device of fluorescent immunochromatography according to claim 1, characterized in that: The top of the chassis (1) is provided with a sample dripping port, the sample dripping port is provided with a cover plate (3), the cover plate (3) is hinged to the chassis (1) at one point, a sealing ring is fixed to the cover plate (3), and a switch (14) is also fixed at the hinged position between the cover plate (3) and the chassis (1), the switch (14) is in a pop-up state when the cover plate (3) is open, and is in a pressed state when the cover plate (3) is closed.

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