Silver drum fish fry transportation method and system

Abstract

The invention belongs to the technical field of aquaculture, and discloses a silver drum fish fry transportation method and system, and the method comprises the steps: planning a transportation route and a transportation date before silver drum fish fry transportation, determining the optimal density of silver drum fish fry transportation through an experiment, and carrying out the transportation through a special fish fry transportation vehicle; in the transportation process, the fish fry activity state and the water quality condition are monitored in real time, and the temperature and the dissolved oxygen condition of the transportation environment are monitored, regulated and controlled in real time; after transportation is finished, the silver drum fish fries are independently discharged into a temporary rearing pond to be cultured and observed for a period of time and then are transferred into a large pond or a net cage to be cultured. The internet and artificial intelligence are combined with the transport vehicle for the first time, real-time dynamic management is achieved, stress to fry is reduced, and the problems that in traditional fry transport, a large amount of manpower needs to be consumed for fry picking and box changing operation, and fry loss is caused in the fry carrying process are solved; the survival rate of fish fry recovery and later-period breeding after transportation is improved, manpower is saved, personal injury is avoided, and breeding income can be greatly improved.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method and a system for transporting silver drum fry. Background Art

[0002] At present, the transportation of fry is an important part of aquaculture production and sales. During the transportation of fry, factors that may cause stress reactions, such as fishing, transportation methods, transportation density, water quality changes, and transportation time, may be involved. The stress response of the fish during transportation will lead to decreased immunity, weak disease resistance, friction damage to the fish, and other problems, which will cause the subsequent fry farming to become ill and stagnate, and in severe cases, a large number of deaths will occur. In order to ensure the healthy development of the industry and reduce unnecessary losses caused by stress reactions during transportation, effective control measures should be taken. It is urgent to study the impact of different factors on the water body and the immune ability of fish during the transportation of fry, so as to determine the best transportation conditions for fry, reduce production losses, and improve economic benefits. Hainan is located in the tropics. At present, many marine fish fry farmed in the country are produced in Hainan, and Hainan supplies 100% of the country's "early breeding" fry. In terms of the number of fry, Hainan accounts for 85% of the egg-shaped pomfret fry and 75% of the grouper fry. Hainan produces seeds for national breeding and then transports them to coastal areas across the country. Therefore, fry transportation technology is particularly important.

[0003] Selenotoca multifasciata, also known as silver drum fish, belongs to the class Actinopterygii, order Perciformes, family Scatophagidae, genus Selenotoca, and is distributed in the southern East my country Sea to the South China Sea and the Beibu Gulf. It can live in seawater, brackish water, saline-alkali water and fresh water. It has strong adaptability, high temperature resistance, fast growth rate, tender meat, omnivorous (partially herbivorous) characteristics, and has ornamental and edible value, important economic development value and broad development prospects. Most of the existing technologies related to Selenotoca multifasciata are about breeding, and there are few studies on the transportation of Selenotoca multifasciata.

[0004] Through the above analysis, the problems and defects of the existing technology are as follows: the existing technology level of most fry transportation is relatively backward, and the equipment directly uses the traditional live fish transport vehicle, the equipment and facilities are relatively simple, and mainly rely on the experience of operators such as fry transport drivers, which is prone to accidents and leads to a large loss of fry. In addition, the excessive attention of the transport driver to the status of the fry can easily lead to transportation accidents. At present, there is no special supporting technology for the transportation of multi-line butterfly fish fry, and it mainly draws on the transportation mode of other varieties, which seriously affects the further demonstration and promotion of this variety. Summary of the invention

[0005] In view of the problems existing in the prior art, the present invention provides a method for transporting silver drum fish fry, and more particularly relates to a method, device, equipment and terminal for transporting silver drum fish fry.

[0006] The present invention is realized in this way. It is a method for transporting silver drum fish fry. The method for transporting silver drum fish fry comprises: planning the best transportation route and transportation date before transporting the silver drum fish fry, determining the best density and other parameters for transporting the silver drum fish fry through experiments, and transporting the silver drum fish fry by using a special fry transport vehicle; during the transportation process, monitoring the activity state of the fry and the water quality in real time, and monitoring and regulating the temperature and dissolved oxygen condition of the transportation environment in real time; after the transportation is completed, temporarily raising the silver drum fish fry separately to recover for a period of time and then transferring them to a breeding pond or a net cage for breeding.

[0007] Further, the silver drum fish fry transportation method comprises the following steps:

[0008] Step 1: Pre-transportation preparation: Plan the best transportation route and transportation date through pre-transportation route planning, distance calculation, and climate correlation of the transportation destination;

[0009] Step 2, transportation process: determine the optimal density and other parameters for transporting silver drum fry through experiments and use a special fry transport vehicle for transportation, and track and observe the activity status of silver drum fry through an intelligent monitoring system and intervene;

[0010] Step 3. Follow-up work after transportation: Keep the silver drum fry alone for a period of time to recover, and then transfer them to the breeding pond for breeding after they have fully adapted to the new environment.

[0011] Furthermore, the preparations before transportation in step one include: planning the route in advance before transporting the fry, and choosing a sunny day for transportation; the density of transporting silver drum fry is within the optimal density range obtained from the experiment; the transportation device is a dedicated fry transport vehicle equipped with a 5G network card, an inflation device and multiple transport nets.

[0012] Furthermore, the transport cages have an Internet control function, and water is changed through the control of a mobile phone Bluetooth app; the carriage has a 360° pan-tilt monitoring camera for real-time monitoring of the activity status of the fry in each cage; each transport cage is equipped with a robotic arm net AI intelligent fishing system, which is connected to a pan-tilt monitoring camera for dynamic capture of the activity status of the fry; the fish are identified by their side flipping and color changes, and the fry that have lost their ability to move are promptly locked and caught, and the information is sent to the staff through the network combined with the monitoring system; a dynamic water quality monitoring system with Internet access is used to monitor the changes in dissolved oxygen, pH, turbidity, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the water in real time to determine the water quality change data.

[0013] Furthermore, the bottom of the cage includes fixed large seaweed, and the cage water body is circulated for transportation. The circulating water flows out of the cage and then enters the filtration system; the filtration system is composed of a small-pore nylon net, sponge, coral stone, activated carbon and zeolite. The filtered water is in the bottom pool and then discharged directly into the cage from the pipe mouth through a pump to a high place without passing through a pipe; the automatic carriage is equipped with a temperature control system, and the optimal transportation temperature is obtained based on experiments; according to the transportation time, a gradual cooling program is formulated, and the temperature is reduced by 1°C at regular intervals. When the transportation time is halfway, the temperature reaches the optimal temperature; then a gradual heating program is formulated according to the temperature of the transportation destination, and the temperature just reaches the temperature in the fish pond when arriving at the destination; each cage has an independent drainage and fish discharge port, which is connected to a pipe to discharge the fish into the fish pond. The fry are fasted one day before transportation, and fry of different sizes are screened in advance.

[0014] Further, the transportation process in step 2 includes: before the fry transport vehicle is assembled and departs, observe whether there are fry in each cage that have died due to stress reaction caused by the handling operation, and if the fry are found dead, remove them in time; observe the activity status of the fry through the monitoring system, check the water quality every 15 minutes, keep the temperature between 22 and 25°C, and the dissolved oxygen is greater than 9.8 mg / L, and remove the dead fry in the cage every 1 hour; when the pH value of the water quality is between 8.2 and 8.8, the ammonia nitrogen content does not exceed 1.80 mg / L, the nitrite content is controlled below 0.18 mg / L, and if the pH value of the water quality, the ammonia nitrogen content and the nitrite are not within the required range, start to replace the water; open the spare water storage tank, discharge one-third of the water volume of the cage with excessive water quality, inject new water, and then observe the changes in water quality; if it is still in an excessive state, inject all fresh seawater for replacement.

[0015] Furthermore, the work after the transportation in step three is completed includes: after the fry are transported to the new site, each cage fish outlet is connected to a fry delivery tube, and the fry are individually discharged into a temporary holding pond for breeding for 7 days; after they are fully adapted to the new environment, they are transferred to the breeding pond or cage for breeding; and the length and weight of the fry are regularly measured to see if they are consistent with the normal growth rate.

[0016] Another object of the present invention is to provide a silver drum fish fry transportation device using the silver drum fish fry transportation method, the silver drum fish fry transportation device comprising a 360° pan-tilt monitoring camera, a transport cage, a dynamic water quality monitoring system, a filtration system, a temperature control system, a spare water storage tank, a 5G network card, an inflation device and a network comprehensive monitoring system.

[0017] Among them, the 360° PTZ surveillance camera is used to monitor the activity status of each transport cage fry in real time;

[0018] The transport cages are equipped with a robotic arm net AI intelligent fishing system and are connected to a 360° PTZ surveillance camera to dynamically capture the activity status of the fry. The fry are identified by their side flipping and color changes, and the fry that have lost their ability to move are promptly locked and scooped up. Each cage has an independent drainage outlet, which is connected to a pipe to drain the fish into the fish pond. The fry are fasted the day before transportation, and the fry of different sizes are screened in advance.

[0019] Dynamic water quality monitoring system, used to monitor the changes of dissolved oxygen, pH, turbidity, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water quality in real time through networking, and determine the water quality change data;

[0020] The filtration system is composed of a small-pore nylon net, sponge, coral stone, activated carbon and zeolite, which is used to filter the circulating water after it flows out of the net box and circulate it from the pipe mouth through the pump to flow into the transport net box;

[0021] The temperature control system is used to formulate a gradual cooling program based on the optimal transportation temperature and transportation time obtained from experiments, reducing the temperature by 1°C at regular intervals, and reaching the optimal temperature when the transportation time is halfway; a gradual heating program is formulated according to the temperature of the transportation destination, so that the temperature reaches the temperature of the fish pond when arriving at the destination;

[0022] The spare water storage cage, when the water pH value, ammonia nitrogen content and nitrite are not within the set requirements, the spare water storage tank is activated to start replacing the water, the water in the cage that exceeds the water quality standard is discharged and new water is injected;

[0023] The network integrated monitoring system is used to send the monitoring information data of the silver drum fish fry transport device to the staff.

[0024] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0025] First, the present invention adopts the Internet plus artificial intelligence in combination with transport vehicles for the first time, which solves the problems of backward facilities and equipment during the transportation of fry, excessive reliance on personnel, and excessive loss of fry, and overcomes the problems of traditional fry transportation that requires a lot of manpower for fry picking, box changing operations, and fry handling process losses, thereby achieving low loss in fry transportation, saving manpower, reducing personal injuries, and increasing breeding income.

[0026] The present invention uses fish school dynamic monitoring technology to timely discover and deal with fry problems during transportation, reduce the contact between fry and people during transportation, reduce stress reactions, and facilitate the recovery of fry breeding in the later stage. In addition, it can prevent transportation personnel from paying too much attention to the status of fry and avoid transportation accidents.

[0027] The present invention adopts a real-time water quality detection method to realize real-time monitoring of water quality changes, timely update water quality, reduce the stress of water quality deterioration on fry, and is also beneficial to the late recovery of fry breeding, so that the fry can arrive at the destination smoothly with low loss.

[0028] The invention determines the key parameters of silver drum fry transportation and the optimal transportation density of the fry.

[0029] The invention provides a method for transporting silver drum fish fry. By equipping with automated facilities and equipment, real-time dynamic management is achieved, the reaction time is greatly shortened, and a series of problems such as mortality rate during the transportation of fish fry, deterioration of water quality, and high mortality rate in subsequent breeding after the transportation are reduced. The method can reduce the loss of silver drum fish fry during transportation, improve the survival rate of the fry, and ensure the success of subsequent breeding.

[0030] Second, as auxiliary evidence of the inventiveness of the claims of the present invention, it is also reflected in the following important aspects:

[0031] (1) The expected benefits and commercial value of the technical solution of the present invention after transformation are:

[0032] The present invention provides a method for transporting silver drum fry, which can effectively reduce the workload of transport personnel, improve the survival rate of fry transportation, reduce labor costs and reduce transportation risks, thereby saving breeding production costs and ensuring the success rate of later breeding.

[0033] (2) The technical solution of the present invention fills the technical gap in the industry at home and abroad:

[0034] The invention provides a silver drum fish fry transportation method, which can greatly reduce the stress reaction of the silver drum fish fry during transportation, avoids artificial contact with the fry during transportation by modern means, can greatly reduce the stress degree of the fry, and is conducive to improving the survival rate of the fry during transportation and the rapid recovery of the fry in the later stage.

[0035] (3) Whether the technical solution of the present invention solves the technical problems that people have been eager to solve but have not been able to solve successfully:

[0036] The invention provides a method for transporting silver drum fish fry. Since the dorsal fin spines of silver drum fish fry contain toxins, manual operation can easily pierce the fry and cause unbearable severe pain. The invention avoids manual operation during transportation, reduces the chance of manual injury, and avoids harm to transportation operators.

[0037] (4) Whether the technical solution of the present invention overcomes technical prejudice:

[0038] The invention provides a method for transporting silver drum fish fry, which can avoid stopping to observe the fry during transportation, greatly reduces the probability of accidents during transportation, and effectively ensures the safety of transportation personnel.

[0039] Third, the silver drum fry transportation method of the present invention effectively solves many technical problems in the prior art in industrial applications and has achieved significant technical progress.

[0040] First of all, a major technical problem often encountered in existing fry transportation methods is that the fry are susceptible to stress during transportation, resulting in a low survival rate. Traditional fry transportation methods often ignore environmental monitoring and fry status observation during transportation, resulting in changes in water quality or lack of oxygen, which in turn affects the health of the fry. The present invention introduces a monitoring system to observe the activity status of silver drum fry and key parameters such as water quality and oxygen concentration in the transportation environment in real time, making the transportation process more controllable. When an abnormal situation is monitored, timely intervention can be carried out to significantly improve the survival rate of the fry.

[0041] Secondly, the present invention optimizes the environmental conditions of the fry during transportation by rationally planning the transportation route and density. Unlike the traditional random transportation method, the present invention emphasizes detailed planning before transportation, including selecting appropriate transportation devices and rationally controlling the density and oxygen supply of the fry. This optimization not only reduces the stress response of the fry during transportation caused by crowding or environmental inadaptability, but also improves transportation efficiency and reduces losses.

[0042] Finally, the present invention significantly reduces the stress response of the fry when they are transferred to the large pond by the step of short-term isolation and cultivation after transportation. In the traditional method, the fry are usually directly placed in the large pond for cultivation after transportation, which is easy to cause stress response or even death due to sudden changes in the environment. The present invention, by setting up an isolation pond, allows the fry to have enough time to adapt to the new environment, provides a smooth transition condition in the initial stage of cultivation, and further improves the survival rate of the fry.

[0043] In summary, the technical solution of the present invention solves the problems of low survival rate of fry and low transportation efficiency in the prior art by optimizing environmental monitoring during transportation, rationally planning transportation density and introducing isolation breeding steps, significantly improves the transportation success rate of silver drum fry, and has important industrial application value.

[0044] Fourthly, the silver drum fry transportation method of the present invention effectively solves multiple technical problems in the prior art through a series of optimized parameters and equipment designs, and achieves significant technical progress.

[0045] First of all, the present invention solves the problem that fish fry are prone to stress reactions due to excessive density, temperature fluctuations and water quality changes during existing fish fry transportation by rationally designing the fish fry transportation density, temperature control system and water quality monitoring system. The optimal transportation density determined by experiments, combined with the intelligent temperature control system that gradually cools down and heats up the compartment, makes the environmental conditions of the fish fry more stable during the entire transportation process. In addition, the real-time water quality monitoring system ensures that parameters such as dissolved oxygen, pH, ammonia nitrogen, and nitrite are always within a reasonable range, greatly reducing the mortality rate of the fish fry. Compared with traditional transportation methods, the present invention achieves precise regulation of the transportation environment through intelligent control and dynamic adjustment, significantly improving the survival rate of the fish fry.

[0046] Secondly, the introduction of the intelligent monitoring and fishing system enables the present invention to monitor the activity status of the fry in real time during transportation, detect problems in time and take corresponding measures. Through the AI ​​intelligent fishing system, the fry that have lost their ability to move are automatically identified and processed, avoiding the problems of untimely manual inspection and low operating efficiency. Through the combination of 5G network and monitoring system, operators can remotely receive fry status and water quality data in real time, and take timely actions, such as performing local water changes or adjusting environmental parameters. This highly automated operation improves transportation efficiency and significantly reduces the loss of fry due to insufficient manual intervention.

[0047] Finally, the present invention effectively solves the adverse effects of water quality deterioration on the survival of fry during long-distance transportation by introducing a water filtration and circulating water system. Through the design of a multi-level filtration device, the water quality can be continuously updated and purified during the entire transportation process, ensuring that the fry are in a healthy water environment. At the same time, the design of an independent drainage and fish discharge port allows the fry to be transferred to the breeding pond more safely after the transportation is completed, avoiding the stress response of the fry caused by sudden environmental changes in the traditional method. This design not only improves the adaptability of the fry, but also reduces the abnormal conditions of the body caused by transportation, ensuring the normal growth of the fry.

[0048] In summary, the present invention has greatly improved the success rate of fry transportation in industrial applications by optimizing key parameters in the transportation process and introducing intelligent monitoring and filtration systems, significantly solved the technical problems in traditional transportation methods, and achieved significant technical progress in ensuring the healthy growth of fry. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0050] Figure 1 The present invention is a flow chart of a method for transporting silver drum fry provided by an embodiment of the present invention.

[0051] Figure 2 They are superoxide dismutase (SOD), catalase (CAT) activities and malondialdehyde (MDA) content of fry after transportation. DETAILED DESCRIPTION

[0052] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0053] In view of the problems existing in the prior art, the present invention provides a method for transporting silver drum fry, which is described in detail below in conjunction with the accompanying drawings.

[0054] like Figure 1 As shown, the method for transporting silver drum fry provided by the embodiment of the present invention comprises the following steps:

[0055] S101, before transporting the silver drum fry, planning the transport route and transport date, and experimentally determining the optimal transport density of the silver drum fry, wherein the transport density is controlled at 12 kg / m 3 ;

[0056] S102, using a special fry transport vehicle for transport, and monitoring the activity status and water quality of the fry in real time during the transportation process, and regulating the temperature and dissolved oxygen level of the transportation environment;

[0057] S103, after the transportation, the silver drum fry are placed in a temporary holding pond for a period of time for cultivation and observation, and then transferred to a large pond or cage for cultivation after they have fully adapted to the new environment.

[0058] The working principle of the silver drum fish fry transportation method can be divided into three main stages. In the pre-transportation preparation stage (S101), the transportation route is planned in advance to ensure the controllability of transportation time and conditions, and reduce the impact of external environmental changes on the fry. The transportation density of the fry is accurately calculated according to the size of the fry, the transportation distance and the conditions of the device to ensure that the fry have enough space and oxygen supply to prevent overcrowding and stress response. Select a suitable transportation device, which usually includes a sealed container with oxygen supply and temperature control functions, to ensure the stability of the fry living environment during the entire transportation process.

[0059] During the monitoring and intervention in the transportation process (S102), the monitoring system in the transport device observes the activity status of the fry in real time, obtains the behavior data of the fry through devices such as cameras and sensors, and monitors key parameters such as water quality, temperature, and oxygen concentration. If the monitoring system detects abnormal activity of the fry or changes in water quality, the transport personnel can intervene in time, such as adjusting the oxygen supply, controlling the temperature, or replacing part of the water body, to maintain a suitable living environment for the fry and ensure that they are in good condition during transportation.

[0060] The fry treatment after transportation (S103) is a key step in the entire transportation process. The silver drum fry being transported are first placed in a separate pond for short-term isolation and culture to avoid sudden transfer to a large pond and causing stress response. The water quality and environment of this isolation pond are consistent with the transportation device, and the temperature and water quality are gradually adjusted to allow the fry to adapt to the new environment. Through this transition period, the fry can gradually recover their physical strength, reduce stress response, and improve the survival rate of subsequent culture.

[0061] Finally, after the fry have fully adapted to the environment in the isolation pool, they can be transferred to a larger and more stable breeding pool. Through the above methods, it is ensured that the silver drum fry are always in a good living state during long-distance transportation, and the survival rate and health status after transportation can be effectively guaranteed, thereby improving the success rate of breeding.

[0062] As a preferred embodiment, the method for transporting silver drum fry provided by the embodiment of the present invention comprises the following steps:

[0063] A. Preparation before transportation: Before transporting the fry, the route should be planned in advance, and the transportation should be carried out on sunny days to avoid weather changes and unstable temperatures. The density of transporting silver drum fry is the optimal density range obtained by the experiment. The transportation vehicle is a special fry transport vehicle equipped with a 5G network card, an air pump, and 20 transport cages. The cages have an Internet control function and can be controlled by a mobile phone Bluetooth app to change water. The carriage has a 360° PTZ monitoring camera, which can monitor the activity status of each cage fry in real time. Each cage is equipped with a mechanical arm net AI intelligent fishing system, connected to a PTZ monitoring camera, which can dynamically capture the activity status of the fry, and judge by the side flipping of the fish body and the color change. The fry that have lost their ability to move are locked in time for fishing and processing. The information is sent to the staff through the network combined with the monitoring system. It has an Internet-connected dynamic water quality monitoring system (dissolved oxygen, pH, turbidity, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen) to grasp the changes in water quality. There are large seaweed fixed at the bottom of the cage. The water in the cage is circulated for transportation. After flowing out of the cage, the circulating water enters the filtration system. The filtration system is composed of small-pore nylon net, sponge, coral stone, activated carbon and zeolite. The filtered water is in the bottom pool, and then pumped to a high place without passing through the pipe, and is directly discharged into the cage from the pipe mouth. The automatic carriage has a temperature control system. The best transportation temperature is obtained according to the experiment. According to the transportation time, a gradual cooling program is formulated. The temperature is reduced by 1°C at regular intervals. When the transportation time is halfway, the temperature reaches the optimal temperature. Then a gradual heating program is formulated according to the temperature of the transportation destination. When arriving at the destination, the temperature just reaches the temperature in the fish pond. Each cage has an independent drainage and fish discharge port, which can be connected to a pipe to discharge the fish into the fish pond. The fry are fasted one day before transportation, and fry of different sizes are screened in advance.

[0064] B. Transportation process: Before the fry transport vehicle is assembled and set off, it is necessary to observe whether there are fry in each cage that have died due to stress caused by handling operations. If fry are found dead, they should be fished out in time to avoid water pollution. During transportation, the activity status of fry should be observed through the monitoring system, and the water quality should be checked every fifteen minutes. The temperature should be maintained at around 22-25℃ during transportation, and the dissolved oxygen should be greater than 9.8 (mg / L). The dead fry in the cage should be fished out every hour or so; the pH value of the water quality should be between 8.2 and 8.8, the ammonia nitrogen content should not exceed 1.80 mg / L (mg / L), and the nitrite content should be controlled below 0.18 mg / L. If the pH value, ammonia nitrogen content, and nitrite of the water quality are not within the required range, start to replace the water, open the spare water storage cage, drain one-third of the water volume of the cage with excessive water quality, inject new water, and then observe the changes in water quality. If it is still in an excessive state, inject all fresh seawater for replacement to keep the water quality fresh. The transport density of fry should be controlled at 12kg / m -3 about.

[0065] C. Subsequent work after transportation: After the fry are transported to the new site, pipes are connected to the fish outlet of each cage, and the fry are individually discharged into a pond for temporary breeding for 7 days. After they are fully adapted to the new environment, they are transferred to a large pond (cage) for breeding. Regularly measure the length and weight of the fry to see if they are consistent with the normal growth rate.

[0066] The embodiment of the present invention uses real-time monitoring and dynamic water quality detection to perform accurate density fry transportation, and reduces fry loss through high-precision dynamic monitoring, which is beneficial to the recovery of fry after transportation and the improvement of survival rate in later breeding.

[0067] Transportation example: Four transportation density groups (G2K, G3.5K, G5K, G6.5K, G8K) are set for the transportation of silver drum fry. The corresponding transportation density is: 6.88kg / m -3 , 12.03kg / m -3 、17.18kg / m -3 , 22.34kg / m -3 , 27.5kg / m -3 ), that is, 2000, 3500, 5000, 6500 or 8000 multi-striped butterfly fish fry are cultured in each fry transport barrel.

[0068] Changes of three enzyme activity indicators in the liver of silver drum fry after transportation experiment: superoxide dismutase (SOD) activity from low to high is G8K, G0K, G2K, G3.5K, G5K, G6.5K, and the lowest is 521.88 U.mgprot in G8K group -1 , the highest G6.5K group was 687.91U.mgprot -1 Compared with the control group (G0K), G5K and G6.5K were significantly higher, while G8K group was significantly lower; G8K group was significantly lower than the other four density groups. The activity of catalase (CAT) was G8K, G6.5K, G0K, G5K, G3.5K, and G2K from low to high, with the lowest activity of G8K group being 17.63 U.mgprot -1 , the highest G2K group was 28.94U.mgprot -1 Compared with the control group, G2K group increased significantly, G5K and G6.5K group showed no difference, G8K group decreased significantly, and G8K group decreased significantly compared with the other four density groups. The trend of malondialdehyde (MDA) content from low to high was consistent with the trend of transport density from small to large, with the lowest G0K group being 1.35nmol.mgprot -1 The highest MDA content in G8K group was 4.38nmol.mgprot -1; Compared with the control group, the five density groups increased significantly, and the G8K group was significantly higher than the other four density groups. The results showed that SOD showed an upward and then downward trend with the increase of experimental density, and CAT showed a downward trend with the increase of experimental density. With the increase of density, the stress of fry increased, resulting in the increase of reactive oxygen free radicals (ROS). The G8K group was damaged by the excessive production of ROS and could not be cleared in time, resulting in the decrease of SOD and CAT activity, which was significantly lower than other density groups and the control group. This shows that silver drum fish fry within a certain density range can clear ROS by improving their own SOD and CAT, so that the body is in a balanced state. The high-density group is more affected and cannot clear too much ROS in time, resulting in a decrease in the body's antioxidant capacity. In this experiment, the MDA content showed an upward trend with the increase of density, and the G8K group was significantly higher than other groups. This shows that excessive high density will force the silver drum fish fry to produce excessive free radicals, which will break the original oxidation balance and induce lipid peroxidation, making the fry prone to excessive stress reaction after transportation, affecting the fry transportation effect. Figure 2 They are SOD, CAT activities and MDA contents of fry after transportation.

[0069] In this transportation example, as the transportation time increases, the deterioration of water quality and the excrement produced by stress will cause breathing difficulties and the body will produce more ROS. The fry body will maintain homeostasis through its own energy supply and the regulation of the immune and antioxidant systems, process the produced ROS, and perform mitochondrial autophagy; the fry at too high a density will be damaged by the friction between the fish bodies due to crowded transportation, excessive energy consumption, and excessive ROS production. The damaged mitochondria cannot be cleared in time, the immune capacity is reduced, the balance is broken, and the damage to the body is accelerated, leading to death. Therefore, the water quality should be kept as fresh as possible during the transportation of fry. During the experiment, although the G2K and G3.5K groups both achieved a 100% transportation survival rate, referring to the changes in the three enzyme activity indicators, considering the survival rate of fry and the economic benefits of transportation, the G3.5K group can transport more fry without affecting the transportation effect. Therefore, the fry transportation density should be controlled at 12kg / m -3 Left and right works best.

[0070] Table 1 Survival rate of fry after transportation

[0071]

[0072] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. A method for transporting silver drum fry, characterized in that: 1) Before transporting silver drum fry, plan the transport route and transport date, and experimentally determine the optimal transport density of silver drum fry, which is controlled at 12kg / m 3 ; 2) Use a special fry transport vehicle for transportation, monitor the activity status and water quality of the fry in real time during transportation, and regulate the temperature and dissolved oxygen level of the transportation environment; 3) After the transportation, the silver drum fry will be placed in a temporary holding pond for observation for a period of time. After they have fully adapted to the new environment, they will be transferred to a large pond or cage for breeding.

2. The method for transporting silver drum fry as claimed in claim 1, characterized in that: The method further comprises: 1) During the transportation process, the activity status of silver drum fry is observed through the monitoring system, and timely intervention is carried out when abnormalities occur; 2) The subsequent steps after the transportation are to isolate the silver drum fry in a temporary holding pond, and then transfer them to a large pond or cage for breeding after they adapt to the new environment to ensure the healthy growth of the fry.

3. The method for transporting silver drum fry as claimed in claim 2, characterized in that: The preparations before transportation in step one include: planning the route in advance before transporting the fry, and choosing a sunny day for transportation; the density of transporting silver drum fry is within the optimal density range obtained from the experiment; the transportation device is a special fry transport vehicle equipped with a 5G network card, an air pump and 20 transport cages.

4. The method for transporting silver drum fry as claimed in claim 3, characterized in that: The transport cages have an Internet control function, and water changes are controlled by a mobile phone Bluetooth app; the carriage is equipped with a 360° pan-tilt monitoring camera for real-time monitoring of the activity status of each caged fry; each transport cage is equipped with a robotic arm net AI intelligent fishing system, which is connected to a pan-tilt monitoring camera for dynamic capture of the activity status of the fry; the fish are identified by their side flipping and color changes, and the fry that have lost their ability to move are promptly locked and fished out, and the information is sent to the staff through the network integrated monitoring system; a dynamic water quality monitoring system with Internet access is used to monitor the changes in dissolved oxygen, pH, turbidity, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the water in real time to determine the water quality change data.

5. The method for transporting silver drum fry as claimed in claim 4, characterized in that: The bottom of the cage includes fixed large seaweed, and the cage water body is circulated for transportation. The circulating water flows out of the cage and then enters the filtration system; the filtration system is composed of small-pore nylon nets, sponges, coral stones, activated carbon and zeolite. The filtered water is in the bottom pool, and then pumped to a high place and discharged directly into the cage from the pipe mouth without passing through pipes; the automatic carriage is equipped with a temperature control system, and the optimal transportation temperature is obtained based on experiments; according to the transportation time, a gradual cooling program is formulated, and the temperature is reduced by 1°C at regular intervals. When the transportation time is halfway, the temperature reaches the optimal temperature; then a gradual heating program is formulated according to the temperature of the transportation destination, and the temperature just reaches the temperature in the fish pond when arriving at the destination; each cage has an independent drainage and fish discharge port, which is connected to a pipe to discharge the fish into the fish pond. The fry are fasted one day before transportation, and fry of different sizes are screened in advance.

6. The method for transporting silver drum fry as claimed in claim 2, characterized in that: The transportation process in step 2 includes: before the fry transport vehicle is assembled and set off, observe whether there are fry in each cage that have died due to stress reaction caused by the handling operation, and if the fry are found dead, remove them in time; observe the activity status of the fry through the monitoring system, check the water quality every 15 minutes, keep the temperature between 22 and 25°C, and the dissolved oxygen is greater than 9.8 mg / L, and remove the dead fry in the cage every 1 hour; when the pH value of the water quality is between 8.2 and 8.8, the ammonia nitrogen content does not exceed 1.80 mg / L, the nitrite content is controlled below 0.18 mg / L, and if the pH value of the water quality, the ammonia nitrogen content and the nitrite content are not within the required range, start to replace the water; open the spare water storage tank, drain one-third of the water volume of the cage with excessive water quality, inject new water, and then observe the changes in water quality; if it is still in an excessive state, inject all fresh seawater for replacement.

7. The method for transporting silver drum fry as claimed in claim 2, characterized in that: The work after the transportation in step three includes: after the fry are transported to the destination, a pipe is connected to the fish outlet of each cage, and the fry are individually discharged into a temporary holding pond for breeding for 7 days; after they have fully adapted to the new environment, they are transferred to a large pond or cage for breeding; and the length and weight of the fry are regularly measured to see if they are consistent with the normal growth rate.

8. A silver drum fish fry transport device using the silver drum fish fry transport method according to any one of claims 1 to 7, characterized in that: The silver drum fry transport device includes a 360° PTZ surveillance camera, a transport cage, a dynamic water quality monitoring system, a filtration system, a temperature control system, a spare water storage tank, a 5G network card, an air pump, and a network integrated monitoring system; Among them, the 360° PTZ surveillance camera is used to monitor the activity status of each transport cage fry in real time; The transport cages are equipped with a robotic arm net AI intelligent fishing system and are connected to a 360° PTZ surveillance camera to dynamically capture the activity status of the fry. The fry are identified by their side flipping and color changes, and the fry that have lost their ability to move are promptly locked and caught. Each cage has an independent drainage port, which is connected to a pipe to drain the fish into the fish pond. The fry are fasted the day before transportation, and the fry of different sizes are screened in advance. Dynamic water quality monitoring system, used to monitor the changes of dissolved oxygen, pH, turbidity, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water quality in real time through networking, and determine the water quality change data; The filtration system is composed of a small-pore nylon net, sponge, coral stone, activated carbon and zeolite, which is used to filter the circulating water after it flows out of the net box and circulate it from the pipe outlet into the transport net box through a pump; The temperature control system is used to formulate a gradual cooling program based on the optimal transportation temperature and transportation time obtained from experiments, reducing the temperature by 1°C at regular intervals, and reaching the optimal temperature when the transportation time is halfway; a gradual heating program is formulated according to the temperature of the transportation destination, so that the temperature reaches the temperature of the fish pond when arriving at the destination; The spare water storage cage is used to replace water when the pH value, ammonia nitrogen content and nitrite content of the water quality are not within the required range, discharge the water in the cage that exceeds the water quality standard and inject new water; The network is combined with a monitoring system to send information about the silver drum fry transport device to the staff.

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