A method and kit for cleaning the surface of ex vivo tissue after magnetic resonance imaging.
By using a combination of hexane, pentane, alcohols, and phosphate buffer, the problem of incomplete cleaning of fluorinated oil on the surface of ex vivo biological tissues after magnetic resonance imaging was solved, achieving rapid and thorough cleaning, simplifying the process, and ensuring the integrity of the biological tissues.
CN119216282BActive Publication Date: 2026-05-26HAINAN UNIV
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HAINAN UNIV
- Filing Date
- 2024-09-29
- Publication Date
- 2026-05-26
AI Technical Summary
Technical Problem
In existing technologies, the cleaning of fluorinated oil on the surface of ex vivo biological tissues after magnetic resonance imaging is incomplete, slow, and complex, affecting subsequent research and processing.
Method used
Fluorinated oil was gradually removed by rinsing with a constant-temperature shaker using a combination of hexane, pentane, alcohol, and phosphate buffer from the kit.
Benefits of technology
It enables rapid and thorough removal of fluorinated oil, simplifies the process, ensures the integrity of biological tissues, and is suitable for different types of biological tissues.
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Figure CN119216282B_ABST
Abstract
This invention discloses a kit for cleaning the surface of ex vivo tissue after magnetic resonance imaging (MRI), comprising reagent A, reagent B, reagent C, and reagent D, wherein: reagent A is a mixture of n-hexane and n-pentane; reagent B is a mixture of n-hexane, n-pentane, and an alcohol; reagent C is an alcohol; and reagent D is a mixture of an alcohol and phosphate buffer. This invention also discloses a method for cleaning the surface of ex vivo tissue after MRI, comprising the step of sequentially rinsing the ex vivo biological tissue sample after MRI using reagents A, B, C, and D from the kit of this invention. This invention introduces n-hexane into the kit to remove perfluoropolyether from the tissue surface, simultaneously solving the technical problems of incomplete cleaning of fluorinated oil from tissue surfaces and slow, complex cleaning processes in existing technologies.
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