Method for preparing organic fertilizer from pigeon feces through efficient and rapid solid-state fermentation

Abstract

The invention discloses a method for preparing an organic fertilizer from pigeon feces through efficient and rapid solid-state fermentation. Comprising the following steps: mixing and solidifying a compounded microbial agent with wood chips or other organic solid organic wastes to prepare a solid composite microbial strain, inoculating the strain into a mixture of pigeon manure, pigeon manure and traditional Chinese medicine residues or pigeon manure and cow manure, fermenting and decomposing at 30-50 DEG C, and drying to prepare an organic fertilizer; the compound microbial agent is prepared from bacillus licheniformis and bacillus clausii. The popularization and application of the organic fertilizer can effectively control the air and environment pollution caused by the pigeon feces, the traditional Chinese medicine residues and the cow dung, avoids a large amount of waste of resources, realizes efficient, green and sustainable utilization of organic wastes, and plays a positive role in enterprise development and social life.

Description

Technical Field

[0001] The invention belongs to the technical field of preparing microbial organic fertilizer, and specifically relates to a method of converting pigeon excrement alone or together with other organic wastes such as Chinese medicine residues, cow dung, etc. into microbial organic fertilizer by using a high-efficiency composite microbial agent under the condition of 30-50°C for efficient and rapid solid-state fermentation, composting and drying. Background Art

[0002] Timely treatment and high-value utilization of pigeon manure has always restricted the green and sustainable development of enterprises. Traditional treatment methods are mainly used for anaerobic fermentation to produce biogas and high-temperature fermentation and composting, but these treatment methods have the disadvantages of long cycle, low efficiency, serious nitrogen loss, high cost, and environmental pollution.

[0003] Based on the current situation that pigeon manure is difficult to efficiently process and utilize as a resource, the present invention uses a composite microbial agent developed specifically to efficiently and rapidly solid-state aerobic ferment pigeon manure alone or together with Chinese medicine residue and cow manure to convert it into microbial organic fertilizer. Pigeon manure provides sufficient nutrients and energy for life activities for the rapid reproduction of microorganisms. Chinese medicine residue and cow manure are organic wastes rich in fiber. After being added, they play a good dispersing role, which is conducive to promoting oxygen mass transfer and water evaporation, and improving the air permeability of the system. The invention comprehensively utilizes the value of pigeon manure, Chinese medicine residue and cow manure, and is an efficient, safe and green treatment process. Summary of the invention

[0004] Aiming at the problem that the traditional method is insufficient in processing pigeon excrement, Chinese medicine residue and cow dung, the invention discloses a method for preparing the excrement into biological organic fertilizer by efficient and rapid solid-state fermentation.

[0005] The invention discloses a method for preparing organic fertilizer by high-efficiency and rapid solid-state fermentation of pigeon feces, comprising the following steps: mixing and solidifying a compounded microbial agent with sawdust or other organic solid waste to prepare a solid composite microbial strain, inoculating the strain into a mixture of pigeon feces, pigeon feces and Chinese medicine residues or pigeon feces and cow dung, fermenting and composting, and drying at a medium-normal temperature (30-50° C.) to prepare the organic fertilizer;

[0006] Preferably, the composite microbial agent comprises Bacillus licheniformis and Bacillus clausii.

[0007] Preferably, the Bacillus licheniformis is Bacillus licheniformis BL-C2B3, with the deposit number being GDMCC No: 64285.

[0008] Preferably, the mixing and solidifying of the compounded microbial agent with sawdust is to solidify the liquid compound microbial agent, stir and mix the compound microbial agent and sterilized sawdust at room temperature in a weight ratio of 1:3, and place them in a dry and ventilated place for natural drying for about 2 hours to obtain solid compound microbial strains of Bacillus licheniformis and Bacillus clausii.

[0009] Preferably, the preparation method of the composite microbial agent is: taking out Bacillus licheniformis or Bacillus clausii and inoculating it into an activation medium, culturing it in a shaker at 37°C and 180rpm for 24h to obtain a seed bacterial solution, then inoculating the seed bacterial solution into a fermentation medium at a volume ratio of 4%, fermenting and culturing it at 37°C for 48h to obtain a fermentation liquid of each strain, and evenly mixing Bacillus licheniformis and Bacillus clausii at a volume ratio of 1:1 to obtain a composite microbial agent;

[0010] The formula of the activation medium and fermentation medium is: glucose 100g / L, NaCl 4.5g / L, yeast extract powder 25g / L, KH2PO42 g / L, anhydrous magnesium sulfate 2g / L, pH 6.0-8.0, and the solvent is ultrapure water.

[0011] Preferably, the solidified composite microbial agent is weighed and inoculated into the pigeon manure, or the mixture of pigeon manure and Chinese medicine residue, or the mixture of pigeon manure and cow dung in the reactor at an inoculation amount of 5% (w / w), and finally the total volume after the mixture accounts for about 70% of the total capacity of the reactor, tap water is added to adjust the moisture content of the mixture to about 70%, the reactor is opened to heat it to 40° C., and it is treated for 24 hours under blast conditions, and then the same amount of pigeon manure is added to continue the above operation, and the pigeon manure is continuously added for fermentation.

[0012] Preferably, the mass ratio of the pigeon manure to the Chinese medicinal residue, or the pigeon manure to cow dung, is 1:1.

[0013] The present invention uses a composite solid microbial agent to rapidly solid-state ferment pigeon feces within 24 hours under normal temperature conditions, and after continuous feeding for 10 batches, the material is discharged to prepare an organic fertilizer with a loose state, uniform color, and qualified degree of maturity. No other waste is discharged during the entire treatment process, and no secondary pollution is generated. The popularization and application of the present invention will effectively control the pollution of pigeon feces, Chinese medicine residues, and cow dung to the air and the environment, avoid a large amount of waste of resources, and achieve efficient, green and sustainable utilization of organic waste, which will have a positive effect on corporate development and social life.

[0014] Bacillus licheniformis BL-C2B3 was deposited in Guangdong Microbiological Culture Collection Center (GDMCC) on January 16, 2024, address: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Postal Code: 510070, and the deposit number is: GDMCC No: 64285. DETAILED DESCRIPTION

[0015] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0016] Experimental materials and equipment: Unless otherwise specified, the experimental reagents and equipment used in the present invention are conventional reagents and equipment.

[0017] Example 1: Preparation of composite microbial agent

[0018] The composite microbial agent is mainly composed of Bacillus licheniformis BL-C2B3 and Bacillus clausii BC-C1B4 (Shouchella clausii BC-C1B4, which is disclosed in NCBI, the accession number is PQ372952.1, which is also held by the applicant and guaranteed to be provided to the public within 20 years from the date of application) isolated by the research team from environmental samples. Bacillus licheniformis has strong stress resistance and wide adaptability, and can produce antibacterial active substances to resist the invasion of pathogens. Bacillus clausii BC-C1B4 has a good control effect on agricultural pathogens and pests, and can be used to prepare fungicides, insecticides and other biological pesticides. At the same time, Bacillus clausii BC-C1B4 can degrade organic pollutants in the soil and has a good protection and repair effect on the soil environment.

[0019] Activation of strains: Take out strains stored at -80°C and inoculate them into activation medium, culture them in a shaker at 37°C and 180rpm for 24h to obtain seed bacterial solution, then inoculate the seed bacterial solution into the fermentation medium at a volume ratio of 4%, and ferment and culture them at 37°C for 48h to obtain the fermentation liquid of each strain. Bacillus licheniformis and Bacillus clausii BC-C1B4 were mixed evenly at a volume ratio of 1:1 to obtain a composite microbial agent.

[0020] Solidification of strains: In order to facilitate storage and transportation, the liquid composite microbial agent is solidified. The composite microbial agent and sterilized sawdust are stirred and mixed at room temperature in a weight ratio of 1:3, and placed in a dry and ventilated place for natural drying for about 2 hours to obtain solid composite microbial strains of Bacillus licheniformis and Bacillus Clausii BC-C1B4.

[0021] The formula of the strain activation medium and the fermentation medium is: glucose 100g / L, NaCl 4.5g / L, yeast extract powder 25g / L, KH2PO42 g / L, anhydrous magnesium sulfate 2g / L, pH 6.0-8.0, and the solvent is ultrapure water. The preparation method of the culture medium is to dissolve and mix the components in ultrapure water, and then sterilize to obtain the required culture medium.

[0022] Example 2: Solid-state fermentation of pigeon manure alone

[0023] Weigh a certain amount of the solidified composite microbial agent in Example 1 and inoculate the strain into the pigeon manure in a reactor with a total capacity of 100 kg at an inoculation rate of 5% (w / w), so that the total volume after mixing accounts for about 70% of the total capacity of the reactor, and add tap water to adjust the water content of the mixed material to about 70%. Open the reactor and heat it to 40°C, treat it under blast conditions for 24 hours, and then add the same amount of pigeon manure to continue the above operation. After adding pigeon manure 5 times in a row, weigh 100g of the treated sample to analyze and detect the required index parameters. The analysis results are shown in Table 1, with a total nitrogen content of 1.88%, a total phosphorus content of 4.93%, a total potassium content of 4.51%, an organic matter content of 44.4%, a water content of 26.65%, and a strain density of 3×10 10 cfu / g. The above index parameters all meet the parameter requirements of the national agricultural industry standard NY / T 798-2015 for compound microbial fertilizers.

[0024] Referring to Appendix D of the national agricultural industry standard NY / T 3442-2019 Technical Specifications for Composting of Livestock and Poultry Manure, a cucumber seed germination rate experiment was carried out on the treated samples. The results showed that the cucumber seed germination rate was 112.5%, indicating that the organic fertilizer prepared by this method has good maturity and is suitable for farmland application.

[0025] Table 1. Content of each component in pigeon droppings after separate treatment

[0026] Inspection items Fermentation products of pigeon droppings after separate processing Nitrogen (on an oven-dry basis) 1.88％ Phosphorus pentoxide (on a dry basis) 4.93％ Potassium oxide (on a dry basis) 4.51％ Mass fraction of organic matter (on an oven-dry basis) 44.4％ Moisture 26.65％ Bacterial density (cfu / g) <![CDATA[3×10 10 ]]> Seed germination index 112.5％

[0027] Example 3: Cooperative solid-state fermentation of pigeon droppings and Chinese medicine residues

[0028] Weigh a certain amount of the solidified composite microbial agent in Example 1 and add it to a reactor with a total capacity of 100 kg at an inoculation rate of 5% (w / w), then add a mixture of pigeon manure and Chinese medicine residue (mainly including licorice, selfheal, chrysanthemum, honeysuckle, etc.) at a weight ratio of 1:1, and finally make the total volume account for about 70% of the total capacity of the reactor, and add tap water to adjust the water content of the mixture to about 70%. Open the reactor and heat it to 40°C, treat it under blast conditions for 24 hours, and then add the same amount of pigeon manure to continue the above operation. After adding pigeon manure 5 times in a row, weigh 100g of the treated sample to analyze and detect the required index parameters. The analysis results are shown in Table 2, total nitrogen content 2.93%, total phosphorus content 1.57%, total potassium content 2.30%, organic matter content 61.0%, water content 9.53%, strain density 5×10 10 cfu / g. The above index parameters all meet the parameter requirements of the national agricultural industry standard NY / T 798-2015 for compound microbial fertilizers.

[0029] Referring to Appendix D of the national agricultural industry standard NY / T 3442-2019 Technical Specifications for Composting of Livestock and Poultry Manure, a cucumber seed germination rate experiment was carried out on the treated samples. The results showed that the cucumber seed germination rate was 108.2%, indicating that the organic fertilizer prepared by this method has good maturity and is suitable for farmland application.

[0030] Table 2. Content of each component after synergistic treatment of pigeon manure and Chinese medicine residue

[0031]

[0032] Example 4: Cooperative solid-state fermentation of pigeon manure and cow manure

[0033] Weigh a certain amount of the cured composite microbial agent in Example 1 and add it to a reactor with a total capacity of 100 kg at an inoculation rate of 5% (w / w), then add a mixture of pigeon manure and cow dung at a weight ratio of 1:1 so that the total volume accounts for about 70% of the total capacity of the reactor, and add tap water to adjust the moisture content of the mixture to about 70%. Open the reactor and heat it to 40°C, treat it under blast conditions for 24 hours, then add the same amount of pigeon manure to continue the above operation, add pigeon manure 5 times in a row, and weigh 100g of the treated sample to analyze and detect the required index parameters. The analysis results are shown in Table 3, total nitrogen content 2.15%, total phosphorus content 4.33%, total potassium content 3.83%, organic matter content 45.5%, water content 28.04%, strain density 3.6×10 10 cfu / g. The above index parameters all meet the parameter requirements of the national agricultural industry standard NY / T 798-2015 for compound microbial fertilizers.

[0034] Referring to Appendix D of the national agricultural industry standard NY / T 3442-2019 Technical Specifications for Composting of Livestock and Poultry Manure, a cucumber seed germination rate experiment was carried out on the treated samples. The results showed that the cucumber seed germination rate was 116.8%, indicating that the organic fertilizer prepared by this method has good maturity and is suitable for farmland application.

[0035] Table 3. Content of each component after synergistic treatment of pigeon manure and cow manure

[0036] Inspection items Fermentation products of pigeon droppings after separate processing Nitrogen (on an oven-dry basis) 2.15％ Phosphorus pentoxide (on a dry basis) 4.33％ Potassium oxide (on a dry basis) 3.83％ Mass fraction of organic matter (on an oven-dry basis) 45.5％ Moisture 28.04％ Bacterial density (cfu / g) <![CDATA[3.6×10 10 ]]> Seed germination index 116.8％

[0037] The above embodiments are all implementation methods with better experimental effects in the present invention, but the implementation methods of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the essence of the principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A method for preparing organic fertilizer by efficient and rapid solid-state fermentation of pigeon feces, characterized in that: The following steps are involved: The compounded microbial agent is mixed and solidified with sawdust or other organic solid waste to obtain a solid composite microbial strain, and the strain is inoculated into a mixture of pigeon feces, pigeon feces and Chinese medicine residues or pigeon feces and cow dung to ferment, decompose and dry at 30-50° C. to prepare an organic fertilizer; The composite microbial agent comprises Bacillus licheniformis and Bacillus clausii.

2. The method according to claim 1, characterized in that The Bacillus licheniformis is Bacillus licheniformis BL-C2B3, and its deposit number is GDMCC No: 64285.

3. The method according to claim 1, characterized in that The method of mixing and solidifying the composite microbial agent with sawdust is to solidify the liquid composite microbial agent, stir and mix the composite microbial agent and sterilized sawdust at room temperature in a weight ratio of 1:3, and place them in a dry and ventilated place for natural drying for about 2 hours to obtain solid composite microbial strains of Bacillus licheniformis and Bacillus clausii.

4. The method according to claim 1, characterized in that The preparation method of the solid composite microbial agent is as follows: Bacillus licheniformis or Bacillus clausii is taken out and inoculated into an activation culture medium, and cultured in a shaker at 37°C and 180 rpm for 24 hours to obtain a seed bacterial liquid, and then the seed bacterial liquid is inoculated into a fermentation culture medium at a volume ratio of 4%, and fermented and cultured at 37°C for 48 hours to obtain a fermentation liquid of each strain, and Bacillus licheniformis and Bacillus clausii are uniformly mixed at a volume ratio of 1:1 to obtain a solid composite microbial agent; The formula of the activation medium and fermentation medium is: glucose 100g / L, NaCl 4.5g / L, yeast extract powder 25g / L, KH2PO42 g / L, anhydrous magnesium sulfate 2g / L, pH 6.0-8.0, and the solvent is ultrapure water.

5. The method according to claim 1, characterized in that The method of inoculating the bacterial strain into a mixture of pigeon feces, pigeon feces and Chinese medicine residues, or pigeon feces and cow dung to ferment, decompose, and dry the mixture under the condition of 30-50 DEG C to prepare organic fertilizer is as follows: weighing the solidified composite microbial agent and inoculating the bacterial strain into the pigeon feces, or the mixture of pigeon feces and Chinese medicine residues, or the mixture of pigeon feces and cow dung in a reactor at an inoculation amount of 5% (w / w), finally making the total volume after the mixture account for about 70% of the total capacity of the reactor, adding tap water to adjust the water content of the mixture to about 70%, opening the reactor to heat it to 40 DEG C, treating it for 24 hours under the condition of blasting, and then adding the same amount of pigeon feces to continue the above operation, and continuously adding pigeon feces for fermentation to obtain organic fertilizer.

6. The method according to claim 1, 2, 3, 4 or 5, characterized in that: The mass ratio of the pigeon droppings to the Chinese medicinal residues, or the pigeon droppings to the cow dung, is 1:1.

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