Microbial remediation agent for hardened soil as well as preparation method and use method of microbial remediation agent

Abstract

The invention provides a hardened soil microbial remediation agent as well as a preparation method and a use method thereof, and relates to the technical field of soil remediation. The hardened soil microbial remediation agent is prepared from the following raw materials in parts by weight: 10-20 parts of functional microorganisms; and 15-30 parts of a carrier. The microbial remediation agent has the functions of dissolving phosphorus, releasing potassium and fixing nitrogen and can promote plants to absorb and utilize nitrogen, potassium and phosphorus in soil, microorganisms can secrete extracellular enzymes in the breeding process, root development is stimulated, nutrient absorption is promoted, and the yield and quality of crops are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of soil remediation, and in particular to a microbial remediation agent for compacted soil and a preparation method and a use method thereof. Background Art

[0002] In recent decades, modern agriculture has developed rapidly, mechanized farming has become popular, and chemical fertilizers have become a guarantee of high yields. However, due to the long-term or single application of chemical fertilizers, no or little application of organic fertilizers, the stability of soil structure has been destroyed, resulting in an increase in soil bulk density and a decrease in porosity. Changes in the basic physical properties of the soil have led to changes in the water holding and water conductivity characteristics of the soil, which has significantly affected the infiltration performance of the soil and changed the storage performance of effective water in the soil. Soil compaction not only reduces the oxygen absorption and nutrient adsorption capacity of the soil, but also reduces the ground temperature, resulting in the inhibition of the activity of aerobic microorganisms in the soil, changing the water, fertilizer, air, and heat environment in the soil, leading to an imbalance in the fertilizer-soil-plant nutrient system, and thus affecting crop growth and farmland environmental quality. In recent years, one of the best remediation products at home and abroad is microbial remediation agents, but microbial remediation agents are of various types and complex, and are not targeted. In addition, the current microbial remediation agent has a relatively simple flora structure and a long repair time. Therefore, it is particularly important to invent a targeted microbial remediation agent for compacted soil to improve soil structure and restore soil fertility. Summary of the invention

[0003] 1. Technical issues to be resolved

[0004] In view of the shortcomings of the prior art, the present invention provides a compacted soil microbial remediation agent and a preparation method and a use method thereof, which solve the problems of the current microbial remediation agent having a relatively single flora structure, a long remediation time, and a lack of highly targeted compacted soil remediation agents on the market.

[0005] (II) Technical solution

[0006] To achieve the above objectives, the present invention is implemented through the following technical scheme: a compacted soil microbial remediation agent, composed of the following raw materials in parts by weight:

[0007] 10-20 portions of functional microorganisms;

[0008] 15 to 30 copies of carrier.

[0009] Preferably, the functional microorganisms include one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus gellingii, Bacillus subtilis, Bacillus amyloliquefaciens, Azotobacter rotundifolia, Lactobacillus plantarum, and Saccharomyces cerevisiae, and the functional microorganisms are all from soil.

[0010] Preferably, the functional microorganism is in powder form, and the amount of viable bacteria per gram is 2 to 8×109 .

[0011] Preferably, the functional microorganism is in powder form, and the amount of viable bacteria per gram is: 3 to 6×10 9 , Bacillus megaterium 2~3×10 9 , Bacillus gelatinosa 2~4×10 9 , Bacillus subtilis 2~3×10 9 , Bacillus amyloliquefaciens 8~10×10 9 , round brown nitrogen-fixing bacteria 1~2×10 9 , Lactobacillus plantarum 2~3×10 9 , Saccharomyces cerevisiae 8~10×10 8 .

[0012] Preferably, the weight ratio of the powder of the functional microorganisms is 1:1:1:1:1:1:1:1:1:1 of Bacillus laterosporus:Bacillus megaterium:Bacillus gellingulatus:Bacillus subtilis:Bacillus amyloliquefaciens:Azotobacter foetida:Lactobacillus plantarum:Saccharomyces cerevisiae.

[0013] Preferably, the carrier includes plant materials and animal materials, the plant materials include one or more of pine needles, corn cobs, and corn stalks, and the animal materials include one or more of shell powder, eggshell powder, and bone powder.

[0014] Preferably, the carrier comprises plant materials and animal materials, the plant materials are pine needles and corn cobs, and the animal materials are shell powder and bone powder.

[0015] Preferably, the weight ratio of plant material to animal material in the carrier is 2:3 or 3:7, and the weight ratio of pine needles: corn cobs: shell powder: bone powder in the carrier is 1:3:3:3 or 1:2:3:4.

[0016] A method for preparing a compacted soil microbial repair agent: the functional microorganisms and carriers are fully stirred and mixed according to the weight ratio of each powder to prepare the agent.

[0017] A method for using a compacted soil microbial repair agent: the repair agent is mixed with the compacted soil and applied.

[0018] (III) Beneficial effects

[0019] The present invention provides a soil microbial repair agent for compacted soil and a preparation method and a use method thereof. It has the following beneficial effects:

[0020] The invention provides a compacted soil microbial repair agent and a preparation method and a use method thereof. The compacted soil microbial improver provided by the invention has the advantages of being environmentally friendly, having a stable effect, and having no secondary pollution. The corresponding functional microorganisms are screened in a targeted manner according to specific soil conditions to construct a functional microbial flora, which can effectively reduce the soil bulk density, increase the total porosity and water retention rate of the soil, and effectively improve the compaction of the soil. In the invention, waste biomass materials are used as carriers for biological adsorption, and the carriers can improve the physical properties of the soil and disperse the soil aggregates, and can also provide sufficient nutrients for the microorganisms, thereby enhancing the repair effect of the microorganisms on the soil, and can effectively shorten the improvement time. The functional microorganisms screened by the invention not only have the functions of dissolving phosphorus, fixing nitrogen, and releasing potassium, and can effectively improve the soil fertility, but also can produce extracellular enzymes during reproduction, stimulate the development of plant roots, and promote the absorption of nutrients by plants, thereby improving crop yield and quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a graph of urease activity analysis of the present invention;

[0022] Figure 2 It is a table chart of alkaline phosphatase activity analysis of the present invention;

[0023] Figure 3 This is a table showing the activity analysis of the sucrase of the present invention;

[0024] Figure 4 This is a chart showing the activity analysis of catalase of the present invention;

[0025] Figure 5 It is an analysis chart of organic matter content of the present invention. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Embodiment 1:

[0028] like Figure 1-5 As shown, a soil compaction microbial repair agent is composed of the following raw materials in parts by weight: 10 parts of functional microorganisms and 15 parts of carriers.

[0029] The functional microorganisms include one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus gellingii, Bacillus subtilis, Bacillus amyloliquefaciens, Azotobacter rotundifolia, Lactobacillus plantarum and Saccharomyces cerevisiae, and the functional microorganisms are all from soil.

[0030] The functional microorganisms are in powder form, and the amount of viable bacteria per gram is 4.87×10 9 .

[0031] The functional microorganism is in powder form, and the amount of live bacteria per gram is: 3.25×10 9 , Bacillus megaterium 3.23×10 9 , Bacillus gelatinosa 2.12×10 9 , Bacillus subtilis 3.26×10 9 , Bacillus amyloliquefaciens 8.43×10 9 , round brown nitrogen-fixing bacteria 1.36×10 9 , Lactobacillus plantarum 2.57×10 9 , Saccharomyces cerevisiae 7.68×10 8 .

[0032] The weight ratio of the powder of the functional microorganisms is 1:1:1:1:1:1:1:1:1:1:1.

[0033] The carrier comprises plant materials and animal materials. The plant materials are pine needles and corn cobs, and the animal materials are shell powder and bone powder.

[0034] The weight ratio of plant material to animal material in the carrier is 3:7.

[0035] The weight ratio of the carrier is pine needles: corn cobs: shell powder: bone powder is 1:2:3:4.

[0036] The functional microorganisms and the carrier are fully stirred and mixed according to the weight ratio of each powder.

[0037] Embodiment 2:

[0038] like Figure 1-5 As shown, a soil compaction microbial repair agent is composed of the following raw materials in parts by weight: 10 parts of functional microorganisms and 30 parts of carriers.

[0039] The functional microorganisms include one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus gellingii, Bacillus subtilis, Bacillus amyloliquefaciens, Azotobacter rotundifolia, Lactobacillus plantarum and Saccharomyces cerevisiae, and the functional microorganisms are all from soil.

[0040] The functional microorganisms are in powder form, and the amount of viable bacteria per gram is 5.49×10 9 .

[0041] The functional microorganism is in powder form, and the amount of live bacteria per gram is: Bacillus laterosporus 6.44×10 9 , Bacillus megaterium 2.35×10 9 , Bacillus gelatinosa 2.42×10 9 , Bacillus subtilis 3.58×10 9 , Bacillus amyloliquefaciens 8.21×10 9 , round brown nitrogen-fixing bacteria 2.69×10 9 , Lactobacillus plantarum 3.38×10 9 , Saccharomyces cerevisiae 8×10 8 .

[0042] The weight ratio of the powder of the functional microorganisms is 1:1:1:1:1:1:1:1:1:1.

[0043] The carrier comprises plant materials and animal materials. The plant materials are pine needles and corn cobs, and the animal materials are shell powder and bone powder.

[0044] The weight ratio of plant material to animal material in the carrier is 2:3.

[0045] The weight ratio of the carrier is pine needles: corn cobs: shell powder: bone powder is 1:3:3:3.

[0046] The functional microorganisms and the carrier are fully stirred and mixed according to the weight ratio of each powder.

[0047] Embodiment 3:

[0048] like Figure 1-5 As shown, a compacted soil microbial remediation agent is composed of the following raw materials in parts by weight: 20 parts of functional microorganisms and 30 parts of carriers.

[0049] The functional microorganisms include one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus gellingii, Bacillus subtilis, Bacillus amyloliquefaciens, Azotobacter rotundifolia, Lactobacillus plantarum and Saccharomyces cerevisiae, and the functional microorganisms are all from soil.

[0050] The functional microorganisms are in powder form, and the amount of viable bacteria per gram is 6.76×10 9 .

[0051] The functional microorganism is in powder form, and the amount of live bacteria per gram is: Bacillus laterosporus 6.24×10 9 , Bacillus megaterium 2.36×10 9 , Bacillus gelatinosa 2.12×10 9 , Bacillus subtilis 3.85×109 , Bacillus amyloliquefaciens 9.43×10 9 , round brown nitrogen-fixing bacteria 2.74×10 9 , Lactobacillus plantarum 3.67×10 9 , Saccharomyces cerevisiae 8.74×10 8 .

[0052] The weight ratio of the powder of the functional microorganisms is 1:1:1:1:1:1:1:1:1:1.

[0053] The raw materials of the carrier include plant materials and animal materials. The plant materials are pine needles and corn cobs, and the animal materials are shell powder and bone powder.

[0054] The weight ratio of plant raw materials to animal raw materials in the carrier is 3:7.

[0055] The weight ratio of the carrier is pine needles: corn cobs: shell powder: bone powder is 1:2:3:4.

[0056] The functional microorganisms and the carrier are fully stirred and mixed according to the weight ratio of each powder.

[0057] Embodiment 4:

[0058] like Figure 1-5 As shown, a compacted soil microbial remediation agent is composed of the following raw materials in parts by weight: 20 parts of functional microorganisms and 30 parts of carriers.

[0059] The functional microorganisms include one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus gellingii, Bacillus subtilis, Bacillus amyloliquefaciens, Azotobacter rotundifolia, Lactobacillus plantarum and Saccharomyces cerevisiae, and the functional microorganisms are all from soil.

[0060] The functional microorganisms are in powder form, and the amount of viable bacteria per gram is 8.22×10 9 .

[0061] The functional microorganism is in powder form, and the amount of live bacteria per gram is: Bacillus laterosporus 4.25×10 9 , Bacillus megaterium 3.23×10 9 , Bacillus gelatinosa 4.28×10 9 , Bacillus subtilis 3.19×10 9 , Bacillus amyloliquefaciens 9.84×10 9 , round brown nitrogen-fixing bacteria 1.42×10 9 , Lactobacillus plantarum 3.27×10 9 , Saccharomyces cerevisiae 9.46×10 8.

[0062] The weight ratio of the powder of the functional microorganisms is 1:1:1:1:1:1:1:1:1:1.

[0063] The carrier comprises plant materials and animal materials. The plant materials are pine needles and corn cobs, and the animal materials are shell powder and bone powder.

[0064] The weight ratio of plant material to animal material in the carrier is 2:3.

[0065] The weight ratio of the carrier is pine needles: corn cobs: shell powder: bone powder is 1:3:3:3.

[0066] The functional microorganisms and the carrier are fully stirred and mixed according to the weight ratio of each powder.

[0067] The compacted soil microbial conditioner prepared above was applied to the compacted soil of the Dingxi potato planting base at 50 kg / mu. Under the same conditions, the compacted soil without the application of the compacted soil microbial conditioner was used as control 1, the biological organic fertilizer purchased from Zhonglin Hi-Tech Biotechnology Co., Ltd. was used as control 2, and the EM bacteria purchased from Jining Xia Nong Biotechnology Co., Ltd. was used as control 3. Soil enzyme activity and organic matter content were measured after applying the compacted soil microbial conditioner for 14 days, 28 days, 42 days and 56 days, respectively. The experimental data obtained above were analyzed and plotted.

[0068] Soil enzymes participate in the cycle of carbon, nitrogen, phosphorus and other nutrients in the soil ecosystem, and are of great significance for maintaining the stability of the soil ecological environment. They are important indicators of soil health and soil fertility. In particular, catalase can be induced by harmful factors in the environment. Catalase activity reflects stress conditions to a certain extent and is one of the indicators of soil degradation. In the last four embodiments, urease (nitrogen), alkaline phosphatase (phosphorus), sucrase (carbon) and catalase (degradation) were measured and analyzed respectively. The results showed that compared with the control group, in the last four embodiments, urease ( Figure 1 ), alkaline phosphatase ( Figure 2 )、Sucrase( Figure 3 ) activities were significantly increased, while catalase ( Figure 4 ) activity is significantly reduced. This shows that the compacted soil conditioner prepared by the method of the present invention can effectively improve soil structure, alleviate soil degradation, and improve soil fertility.

[0069] Soil organic matter is an important component of soil and an important indicator for measuring soil fertility. Figure 5) were analyzed, and the results showed that: in the four different embodiments, the soil organic matter was greatly improved compared with the control group. This shows that the compacted soil conditioner prepared by the method described in the present invention can increase the decomposition and conversion of soil nutrients and improve the content of soil organic matter.

[0070] In the urease activity table of the control group and the embodiment, A, B, C, and D are urease activities a, b, c, d, e, f, and g at 14 days, 28 days, 42 days, and 56 days, respectively, indicating that there are significant differences between different groups.

[0071] In the alkaline phosphatase activity table of the control group and the embodiment, A, B, C, and D are the alkaline phosphatase activities a, b, c, d, e, and f at 14 days, 28 days, 42 days, and 56 days, respectively, indicating that there are significant differences between different groups.

[0072] In the table of sucrase activity of the control group and the examples, A, B, C, and D are sucrase activities a, b, c, d, e, and f at 14 days, 28 days, 42 days, and 56 days, respectively, indicating that there are significant differences between different groups.

[0073] In the table of catalase activity of the control group and the embodiment, A, B, C, and D are the catalase activities a, b, c, d, e, f, and g at 14 days, 28 days, 42 days, and 56 days, respectively, indicating that there are significant differences between different groups.

[0074] In the table of organic matter content in the control group and the embodiment, A, B, C, and D are respectively the organic matter contents a, b, c, d, e, f, and g at 14 days, 28 days, 42 days, and 56 days, indicating that there are significant differences between different groups.

[0075] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A soil microbial remediation agent for compacted soil, characterized in that: It is composed of the following raw materials in parts by weight: 10-20 portions of functional microorganisms; 15 to 30 copies of carrier.

2. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The functional microorganisms include one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus gellingii, Bacillus subtilis, Bacillus amyloliquefaciens, Azotobacter rotundifolia, Lactobacillus plantarum and Saccharomyces cerevisiae, and the functional microorganisms are all from soil.

3. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The functional microorganisms are in powder form, and the amount of live bacteria per gram is 2 to 8×10 9 .

4. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The functional microorganism is in powder form, and the amount of live bacteria per gram is: 3 to 6×10 9 , Bacillus megaterium 2~3×10 9 , Bacillus gelatinosa 2~4×10 9 , Bacillus subtilis 2~3×10 9 , Bacillus amyloliquefaciens 8~10×10 9 , round brown nitrogen-fixing bacteria 1~2×10 9 , Lactobacillus plantarum 2~3×10 9 , Saccharomyces cerevisiae 8~10×10 8 .

5. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The weight ratio of the powder of the functional microorganisms is 1:1:1:1:1:1:1:1:1:

1.

6. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The carrier includes plant materials and animal materials. The plant materials include one or more of pine needles, corn cobs, and corn stalks. The animal materials include one or more of shell powder, eggshell powder, and bone powder.

7. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The carrier comprises plant materials and animal materials. The plant materials are pine needles and corn cobs, and the animal materials are shell powder and bone powder.

8. The microbial remediation agent for compacted soil according to claim 1, characterized in that: The weight ratio of plant material to animal material in the carrier is 2:3 or 3:7, and the weight ratio of pine needles: corn cobs: shell powder: bone powder in the carrier is 1:3:3:3 or 1:2:3:

4.

9. A method for preparing a microbial remediation agent for compacted soil, characterized in that: The functional microorganisms and the carrier are fully stirred and mixed according to the weight ratio of each powder.

10. A method for using a compacted soil microbial remediation agent, characterized in that: The repair agent is mixed with compacted soil and applied.

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