Detection consumable and detection equipment

Abstract

The invention provides a detection consumable and detection equipment. A reaction tube is provided with a detection cavity for accommodating a detection reagent and a sealing film for sealing the detection cavity; the sample adding piston is contained in the cavity and can sequentially move from an initial position to a sample adding position and a quantitative position relative to the sealing film, the sample adding piston located at the initial position and the sealing film are arranged at an interval, so that the detection cavity and the cavity are mutually isolated, and the sample adding piston located at the sample adding position abuts against the sealing film and does not penetrate through the sealing film; the sample adding piston at the quantitative position is in limiting fit with the sealing film and seals the detection cavity. According to the invention, the sample adding piston is pressed against the sample adding piston, so that the sample adding piston can sequentially move from the initial position to the sample adding position and the quantitative position, a sample liquid in the cavity can be quantitatively guided into the detection cavity, and after the sample liquid is guided into the detection cavity, the sample adding piston can seal the detection cavity, so that the flow of transferring a solution between containers can be avoided, and the detection accuracy and the experiment efficiency are improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of in vitro diagnosis, and in particular relates to a detection consumable and a detection device. Background Art

[0002] When a sample (such as sputum, urine, or blood) needs to be tested, it is usually manually drawn into a sampling tube and a corresponding volume of processing liquid is added to each sample for processing. However, when the number of samples to be tested is large, it is difficult for current testing consumables to add different volumes of processing liquid according to the volume of each sample, resulting in low sample testing accuracy.

[0003] In addition, current testing consumables only have the function of solution transfer. During the testing experiment, not only do samples need to be placed in processing fluids such as cell preservation fluid for preservation, but the mixed solution must also be manually aspirated from one container and then transferred to another container in order to achieve subsequent testing processes such as nucleic acid extraction or PCR amplification. As a result, the experimental process requires multiple steps, is prone to errors and is costly. Summary of the invention

[0004] The main purpose of the present invention is to provide a detection consumable and a detection device, aiming to solve the technical problems of low sample addition accuracy and complex operation procedures of the detection consumable in the prior art.

[0005] In order to achieve the above-mentioned purpose, the present invention provides a detection consumable, which includes: a sample tube, which is provided with a cavity for accommodating a sample liquid; a reaction tube, which is connected to the sample tube, and the reaction tube is provided with a detection cavity for accommodating a detection reagent and a sealing film that seals the detection cavity; a sample adding piston, which is accommodated in the cavity, and the sample adding piston can move from an initial position to a sample adding position and a quantitative position relative to the sealing film in sequence, the sample adding piston and the sealing film located at the initial position are arranged at intervals, so that the detection cavity and the cavity are isolated from each other, the sample adding piston and the sealing film located at the sample adding position abut against and do not penetrate the sealing film, the sample adding piston and the sealing film located at the quantitative position limit and cooperate with each other to close the detection cavity, and during the movement of the sample adding piston between the sample adding position and the quantitative position, the detection cavity is connected to the cavity, and the sample liquid can be quantitatively introduced into the detection cavity.

[0006] In an embodiment of the present invention, the cavity includes: a sample area, which is used to accommodate the sample liquid; a piston area, which is arranged side by side with the sample area and connected through a liquid inlet, and the sample adding piston located at the initial position is accommodated in the piston area; a quantitative area, which is located between the piston area and the detection cavity, and the quantitative area is used to seal and cooperate with the sample adding piston in the quantitative position and close the detection cavity.

[0007] In an embodiment of the present invention, the sample adding piston comprises: a piston rod, a puncturing tip is provided at one end close to the reaction tube, and the puncturing tip is used to puncture the sealing film; a sealing sleeve is sleeved outside the piston rod and is used to seal with the quantitative area.

[0008] In an embodiment of the present invention, the sample tube includes: a main body tube, the sample area and the piston area are both arranged in the main body tube; a connecting tube, which is connected to the main body tube and the cross-sectional size of the connecting tube is smaller than the cross-sectional size of the main body tube, the quantitative area is arranged in the connecting tube, the reaction tube is provided with an installation cavity for the connecting tube to extend into, the sealing film is located between the installation cavity and the detection cavity, and the end of the connecting tube away from the main body tube abuts against the sealing film.

[0009] In an embodiment of the present invention, the liquid inlet passes through the piston area along the length direction of the piston area, and the length of the sample adding piston is smaller than the length of the liquid inlet.

[0010] In an embodiment of the present invention, an opening is provided at one end of the cavity away from the detection cavity, and the detection consumable further comprises a split sealing cover for opening or closing the opening.

[0011] In an embodiment of the present invention, the split sealing cover includes: a cover body, which is detachably connected to the sample tube and the cover body is provided with an avoidance cavity for the sample tube to extend into; a pressure column, which is accommodated in the avoidance cavity and connected to the cover body, and the pressure column is used to press the sample adding piston in a direction close to the detection cavity.

[0012] In an embodiment of the present invention, the outer wall of the sample tube is provided with a breaking structure for abutting the cover body, and the cover body is used to break the breaking structure and separate the breaking structure from the sample tube when the pressing column presses the sample adding piston.

[0013] In an embodiment of the present invention, a limiting ring is further provided on the outer wall of the sample tube, and the limiting ring is used to limit the cover body in the quantitative position.

[0014] The present invention also discloses a detection device, which includes the detection consumables as described above.

[0015] Through the above technical solution, the detection consumables provided by the embodiment of the present invention have the following beneficial effects:

[0016] When using the detection consumables, the detection cavity in the reaction tube is preset with detection reagents, and the sealing film can seal the detection reagents in the detection cavity. Sample liquid or sampling swab can be added to the cavity of the sample tube, and cell preservation liquid can be preset in the cavity as a processing liquid. At this time, the sample adding piston is in the initial position, and the sample adding piston and the sealing film do not contact each other and are arranged at intervals. When the sample liquid needs to be tested, the sample adding piston can be pressed to make the sample adding piston move from the initial position to the sample adding position and the quantitative position in sequence. The sample adding piston in the sample adding position is in contact with the sealing film and does not penetrate the sealing film. Continuing to press the sample adding piston toward the quantitative position can make the sample adding piston pierce the sealing film, so that the detection cavity and part of the cavity are connected, and the sample liquid can be introduced from the part of the cavity into the detection cavity and communicate with the detection liquid in the detection cavity. After the reagents are mixed, the sample adding piston continues to be pressed to the quantitative position. The sample adding piston in the quantitative position can close the detection cavity. The cavity and the detection cavity are isolated by the sample adding piston, so that the cavity and the detection cavity are isolated, and the sample liquid cannot enter the detection cavity from the cavity. Since the cross-sectional size of the sample tube is fixed, the distance between the sample adding position and the quantitative position is constant, and the sealed space formed by the sample adding piston and the sample tube is quantitative, which can ensure that the volume of the solution entering the detection cavity is the set quantitative volume. After the solution is injected, the sealed environment in the detection cavity can also be ensured, which is convenient for further testing of the solution of the quantitative volume, and can avoid the process of external transfer of the solution between containers in the prior art, reduce the risk of contamination of the detection reagent and the sample, and improve the accuracy of the detection and the experimental efficiency. The detection consumables of the present invention cooperate with three components: a sample tube, a reaction tube and a sample adding piston, so that the detection chamber in the reaction tube can be pre-filled with detection reagents, and can be isolated from the cavity by a sealing film. Pressing the sample adding piston can make the sample adding piston move from an initial position to a sample adding position and a quantitative position in sequence, so that the sample liquid in the cavity can be quantitatively introduced into the detection chamber. After the sample liquid is introduced into the detection chamber, the sample adding piston can seal the detection chamber, so that the process of transferring solutions between containers can be avoided, thereby improving the accuracy of detection and the experimental efficiency.

[0017] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide an understanding of the present invention and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present invention, but do not constitute a limitation of the present invention. In the accompanying drawings:

[0019] Figure 1 is a schematic diagram of the structure of a detection consumable according to an embodiment of the present invention;

[0020] Figure 2 is a schematic structural diagram of a sample tube for testing consumables according to an embodiment of the present invention at a certain viewing angle;

[0021] Figure 3is a schematic structural diagram of a sample tube for detecting consumables according to an embodiment of the present invention from another viewing angle;

[0022] Figure 4 is a schematic diagram of the cross-sectional structure of a reaction tube of a detection consumable according to an embodiment of the present invention;

[0023] Figure 5 is a schematic diagram of the structure of a sample adding piston for detecting consumables according to an embodiment of the present invention;

[0024] Figure 6 It is a schematic diagram of the structure of a split sealing cover for detecting consumables according to an embodiment of the present invention;

[0025] Figure 7 is a schematic diagram of a cross-sectional structure of a detection consumable in one state according to an embodiment of the present invention;

[0026] Figure 8 is a schematic diagram of a cross-sectional structure of a detection consumable in another state according to an embodiment of the present invention;

[0027] Fig. 9 2 is a schematic diagram of the cross-sectional structure of a detection consumable in another state according to an embodiment of the present invention.

[0028] Description of Reference Numerals

[0029] Label Name Label Name

[0030] 100 Testing consumables 31 Piston rod

[0031] 1 Sample tube 32 Piercing tip

[0032] 11 Cavity 321 Puncture flow channel

[0033] 111 Sample area 33 Sealing sleeve

[0034] 112 Piston area 4 split sealing cover

[0035] 113 quantitative area 41 cover

[0036] 12 Main body tube 411 Avoidance cavity

[0037] 121 broken structure 4111 sealing section

[0038] 122 Limiting ring 4112 Transition section

[0039] 13 Connecting tube 4113 Avoidance section

[0040] 14 Open 42 Pressure column

[0041] 15 Sealing ring 421 Column

[0042] 2 Reaction tube 422 Strengthening edge

[0043] 21 Detection chamber 4221 Connecting bending part

[0044] 22 Sealing film 4222 Edge body

[0045] 23 Mounting cavity 200 Sampling piece

[0046] 3 Piston DETAILED DESCRIPTION

[0047] The specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0048] The detection consumable according to the present invention will be described below with reference to the accompanying drawings.

[0049] like Figures 1 to 6 As shown, in an embodiment of the present invention, the detection consumable 100 includes a sample tube 1, a reaction tube 2 and a sample adding piston 3, the sample tube 1 is provided with a cavity 11 for accommodating a sample liquid; the reaction tube 2 is connected to the sample tube 1, and the reaction tube 2 is provided with a detection cavity 21 for accommodating a detection reagent and a sealing film 22 for sealing the detection cavity 21; the sample adding piston 3 is accommodated in the cavity 11, and the sample adding piston 3 can move from an initial position to a sample adding position and a quantitative position in sequence relative to the sealing film 22, the sample adding piston 3 and the sealing film 22 at the initial position are arranged at intervals, so that the detection cavity 21 and the cavity 11 are isolated from each other, the sample adding piston 3 and the sealing film 22 at the sample adding position abut and do not penetrate the sealing film 22, the sample adding piston 3 and the sealing film 22 at the quantitative position limit and cooperate to close the detection cavity 21, and during the movement of the sample adding piston 3 between the sample adding position and the quantitative position, the detection cavity 21 is connected to the cavity 11, and the sample liquid can be quantitatively introduced into the detection cavity 21.

[0050] It can be understood that the detection consumables 100 in this embodiment are mainly used for the extraction and amplification of nucleic acids. The reaction tube 2 can be pre-installed with a freeze-dried reagent as a detection reagent. A sampling swab can be used as a sampling piece 200 for sample sampling. After the sampling is completed, the sampling piece 200 can be placed in the cavity 11 of the sample tube 1, and a cell preservation fluid can be provided in the cavity 11.

[0051] When using the detection consumables 100 in this embodiment, the detection chamber 21 in the reaction tube 2 is pre-set with detection reagents, and the sealing film 22 can seal the detection reagents in the detection chamber 21. Sample liquid can be added to the cavity 11 of the sample tube 1, and the cavity 11 can be pre-set with cell preservation liquid as a processing liquid. At this time, the sample adding piston 3 is in the initial position, and the sample adding piston 3 and the sealing film 22 do not contact each other and are arranged at intervals. When the sample liquid needs to be detected, the sample adding piston 3 can be pressed to make the sample adding piston 3 move from the initial position to the sample adding position and the quantitative position in sequence. The sample adding piston 3 in the sample adding position is in contact with the sealing film 22 and does not penetrate the sealing film 22. Continuing to press the sample adding piston 3 toward the quantitative position can make the sample adding piston 3 pierce the sealing film 22, so that the detection chamber 21 is connected with part of the cavity 11, and the sample liquid can be introduced from the part of the cavity 11 into the detection chamber 21 and communicate with the detection chamber 21. The detection reagents in the detection chamber 21 are mixed, and the sample adding piston 3 is continuously pressed to the quantitative position. The sample adding piston 3 in the quantitative position can close the detection chamber 21, and the cavity 11 and the detection chamber 21 are isolated by the sample adding piston 3, so that the cavity 11 and the detection chamber 21 are isolated, and the sample liquid cannot enter the detection chamber 21 from the cavity 11. Since the cross-sectional size of the sample tube 1 is fixed, the distance between the sample adding position and the quantitative position is constant, and the sealed space formed by the sample adding piston 3 and the sample tube 1 is quantitative, which can ensure that the volume of the solution entering the detection chamber 21 is the set quantitative volume. After the solution is injected, it can also ensure that the sealing ring 15 in the detection chamber 21 is in the environment, which is convenient for further testing of the solution of the quantitative volume, and can avoid the process of external transfer of the solution between containers in the prior art, reduce the risk of contamination of the detection reagent and the sample, and improve the accuracy of the detection and the experimental efficiency. In this embodiment, the detection consumables 100 cooperates with the three components of the sample tube 1, the reaction tube 2 and the sample adding piston 3, so that the detection chamber 21 in the reaction tube 2 can be pre-filled with detection reagents, and can be isolated from the cavity 11 by the sealing film 22. Pressing the sample adding piston 3 can make the sample adding piston 3 move from the initial position to the sample adding position and the quantitative position in sequence, so that the sample liquid in the cavity 11 can be quantitatively introduced into the detection chamber 21. After the sample liquid is introduced into the detection chamber 21, the sample adding piston 3 can seal the detection chamber 21, thereby avoiding the process of transferring solutions between containers, thereby improving the accuracy of detection and the experimental efficiency.

[0052] In one embodiment, if Figures 7 to 9As shown, the cavity 11 includes a sample area 111, a piston area 112 and a quantitative area 113. The sample area 111 is used to accommodate the sample liquid; the piston area 112 and the sample area 111 are arranged side by side and connected through the liquid inlet, and the sample adding piston 3 located at the initial position is accommodated in the piston area 112; the quantitative area 113 is located between the piston area 112 and the detection chamber 21, and the quantitative area 113 is used to seal and cooperate with the sample adding piston 3 in the quantitative position and close the detection chamber 21. When the sample adding piston 3 is in the piston area 112 and has not pierced the sealing film 22, the sample liquid in the sample area 111 can enter the quantitative area 113 through the liquid inlet and the piston area 112 in sequence, so that the quantitative solution is stored in the quantitative area 113. The quantitative area 113 is located below the liquid inlet. When the sample adding piston 3 moves to the quantitative area 113, the sealing film 22 is pierced and the liquid inlet is blocked, so that the quantitative area 113 and the piston area 112 are isolated, and the solution in the sample area 111 cannot enter the quantitative area 113 through the liquid inlet. The sample adding piston 3 continues to be pressed downward, and the quantitative solution in the quantitative area 113 can be pressed into the detection cavity 21 until the sample adding piston 3 moves to the quantitative position and completely blocks the quantitative area 113.

[0053] The sample tube 1 can extend in the up-down direction, the reaction tube 2 is connected to the lower end of the sample tube 1, the piston area 112 and the sample area 111 both extend in the up-down direction, the quantitative area 113 is located at the lower ends of the sample area 111 and the piston area 112, and the sealing film 22 is located at the lower end of the quantitative area 113. When the sample adding piston 3 is pressed downward, the sample adding piston 3 located at the initial position can move to the sample adding position abutting the sealing film 22, directly pressing the sample adding piston 3, and the sample adding piston 3 can pierce the sealing film 22, so that the sample liquid in the sample area 111 can enter the detection chamber 21 through the piston area 112 and the quantitative area 113 in sequence. The sample area 111 and the piston area 112 in this embodiment are arranged side by side, which can facilitate the placement of the sample adding piston 3 and the sampling member 200, and can also improve the compactness of the structure of the detection consumable 100. In this embodiment, the shape and size of the sample area 111, the piston area 112 and the quantitative area 113 can be set according to the actual use situation. In order to facilitate quantitative, the sample area can be correspondingly provided with a solution scale.

[0054] like Figure 5As shown, the sample adding piston 3 includes a piston rod 31 and a sealing sleeve 33. The end of the piston rod 31 close to the reaction tube 2 is provided with a piercing tip 32, and the piercing tip 32 is used to pierce the sealing film 22; the sealing sleeve 33 is sleeved outside the piston rod 31 and is used to seal with the quantitative area 113. In this embodiment, the lower end of the piston rod 31 may be provided with a mounting groove for the sealing sleeve 33 to extend into, the piston rod 31 and the sealing sleeve 33 can be integrally formed, the sealing sleeve 33 can be made of soft rubber, when the sample adding piston 3 moves to the quantitative position, the piercing tip 32 extends into the detection chamber 21, the sealing sleeve 33 and the quantitative area 113 are sealed together to completely close the detection chamber 21, the piercing tip 32 is arranged below the sealing sleeve 33, and the piercing tip 32 may be provided with a piercing channel 321 for the solution to flow through, the extension direction of the piercing channel 321 is consistent with the extension direction of the piercing tip 32, when the piercing tip 32 extends into the detection chamber 21, the solution from the sample area 111 to the quantitative area 113 through the piston area 112 can enter the detection chamber 21 through the piercing channel 321.

[0055] like Figure 7 As shown, the sample adding piston 3 is in the initial position, and the cavity 11 and the detection cavity 21 are isolated from each other. Figure 8 As shown, the sample adding piston 3 is between the sample adding position and the quantitative position, the sealing film 22 is pierced by the piercing tip 32, and the solution in the sample area 111 can enter the piercing channel 321 through the piston area 112, and enter the detection chamber 21 through the piercing channel 321. Fig. 9 As shown, the sample adding piston 3 is in the quantitative position, and the sample adding piston 3 completely seals the sealing film 22. The lower end of the puncture flow channel 321 is provided with a converging slope converging to the tip of the puncture tip 32, which can facilitate the solution to flow along the puncture tip 32.

[0056] like Figure 1 and Figures 7 to 9 As shown, an opening 14 is provided at one end of the cavity 11 away from the detection cavity 21, and the detection consumable 100 further includes a split sealing cover 4 for opening or closing the opening 14. Before adding a sample to the detection consumable 100, the split sealing cover 4 can be used to close the opening 14, so that the cavity 11 is in a sealed state. When adding a sample is required, the split sealing cover 4 can be used to open the opening 14, so that the sampling member 200 can enter the cavity 11 through the opening 14. After the sampling member 200 is placed, the split sealing cover 4 can be used to close the opening 14, so that the detection consumable 100 is in a sealed state as a whole, which can avoid external environmental pollution.

[0057] In the embodiment of the present invention, Figure 6As shown, the split sealing cover 4 includes a cover body 41 and a pressure column 42. The cover body 41 and the sample tube 1 are detachably connected and the cover body 41 is provided with an avoidance cavity 411 for the sample tube 1 to extend into; the pressure column 42 is accommodated in the avoidance cavity 411 and connected to the cover body 41, and the pressure column 42 is used to press the sample adding piston 3 in the direction close to the detection cavity 21.

[0058] When using the detection consumables 100 in this embodiment, the opening 14 of the cavity 11 is opened by operating the cover body 41, and the sample liquid can be injected into the cavity 11 through the opening 14. Then, the opening 14 can be closed by the cover body 41, so that the cavity 11 in the sample tube 1 is in a sealed state. The cover body 41 can move relative to the sample tube 1, so that the pressure column 42 connected to the cover body 41 presses the sample adding piston 3. There is no need to remove the sample adding piston 3, which can ensure that the sample adding piston 3 is always in the cavity, and can avoid the sample adding piston 3 from contacting with the external environment and being contaminated. The sample adding piston 3 can pierce the sealing film 22 under the pressure of the pressure column 42, so that the sample liquid in the cavity 11 can enter the detection cavity 21. In this embodiment, the pressure column 42 and the cover body 41 are connected, and the pressure column 42 and the sample adding piston 3 are designed in sections. When the cover body 41 is operated externally, the pressure column 42 and the sample adding piston 3 can be driven to move synchronously. The sample adding piston 3 can always be kept in the cavity 11, and the sample adding piston 3 can be prevented from being exposed to the outside environment. The sealing is good and the detection accuracy can be improved.

[0059] In one embodiment, the piston rod 31 is used to abut against the pressing column 42, and the cross-sectional size of the puncturing tip 32 is smaller than the cross-sectional size of the piston rod 31. The outer periphery of the piston rod 31 is provided with a guide ridge, which can be guided and matched with the tube wall of the sample tube 1, and a weight-reducing groove is formed between the plurality of guide ridges. The length of the guide ridge is the same as the length of the piston rod 31. The length of the piston rod 31 and the length of the puncturing tip 32 can be set according to actual use requirements.

[0060] like Figure 2 and Figure 3 As shown, the sample tube 1 includes a main body tube 12 and a connecting tube 13, and the sample area 111 and the piston area 112 are both arranged in the main body tube 12; the connecting tube 13 is connected to the main body tube 12 and the cross-sectional size of the connecting tube 13 is smaller than the cross-sectional size of the main body tube 12, the quantitative area 113 is arranged in the connecting tube 13, and the reaction tube 2 is provided with an installation cavity 23 for the connecting tube 13 to extend into, the sealing film 22 is located between the installation cavity 23 and the detection cavity 21, and the end of the connecting tube 13 away from the main body tube 12 abuts against the sealing film 22.

[0061] The sample tube 1 in this embodiment is in the shape of a large-small body, and the sample area 111 and the piston area 112 are arranged in the main body tube 12 at the upper end, and the quantitative area 113 is arranged in the connecting tube 13 at the lower end. At the same time, the connecting tube 13 is extended into the installation cavity 23 in the reaction tube 2, which can facilitate the connection between the sample tube 1 and the reaction tube 2. The connecting tube 13 in this embodiment can be interference fit with the installation cavity 23, or can be connected to the installation cavity 23 by thread, or connected by a connector such as a buckle or a screw. The sealing film 22 is located between the installation cavity 23 and the detection cavity 21 and abuts against the connecting tube 13, which can facilitate the assembly and positioning of the connecting tube 13. When the sealing sleeve 33 and the quantitative area 113 are sealed, the connecting tube 13 can cooperate with the sealing sleeve 33 to seal the detection cavity 21, thereby improving the sealing stability.

[0062] In one embodiment, the liquid inlet penetrates the piston area 112 along the length direction of the piston area 112, and the length of the sample adding piston 3 is less than the length of the liquid inlet. In this embodiment, the liquid inlet is located at the side of the piston area 112, and the sample adding piston 3 can extend in the up-down direction, and the length is less than the length of the liquid inlet, which can avoid the situation where the sample adding piston 3 at the initial position is exposed, so that both ends of the sample adding piston 3 at the initial position are accommodated in the piston area 112, which can avoid the situation where the sample adding piston 3 is accidentally touched by external components and causes inaccurate detection.

[0063] When it is necessary to press the sample adding piston 3 to move the sample adding piston 3 in the initial position to the sample adding position, the cover body 41 can be pressed outside the detection consumable 100 to make the cover body 41 move along the extension direction of the sample tube 1. At the same time, the length of the sample tube 1 extending into the avoidance cavity 411 gradually becomes longer. The pressing column 42 can press the sample adding piston 3 under the action of external force to move the sample adding piston 3, and the cover body 41 can always keep the cavity 11 of the sample tube 1 in a closed state. In this embodiment, the solution can be transferred without opening the sample tube 1, which can improve the sample detection accuracy.

[0064] In one embodiment, the outer wall of the sample tube 1 is provided with a breaking structure 121 for abutting against the cover body 41, and the cover body 41 is used to break the breaking structure 121 and separate the breaking structure 121 from the sample tube 1 when the pressing column 42 presses the sample adding piston 3. The breaking structure 121 in this embodiment is in the shape of a flat plate, and a breaking slope is provided at the connection between the breaking structure 121 and the cover body 41, and the cross-sectional size of the breaking slope gradually decreases toward the direction close to the outer wall of the sample tube 1, which can facilitate the breaking of the breaking structure 121.

[0065] like Figure 7 In the present embodiment, the sample adding piston 3 is in the initial position, the cover body 41 abuts against the breaking structure 121 and does not break the breaking structure 121. When it is necessary to press the sample adding piston 3, an external force can be used to push the cover body 41 so that the cover body 41 breaks the breaking structure 121. Figure 8As shown, the situation that the pressing column 42 on the cover body 41 presses the sample adding piston 3 in case of accidental contact can be avoided.

[0066] like Figures 1 to 3 As shown, the outer wall of the sample tube 1 is further provided with a limiting ring 122, which is used to limit the cover body 41 in the quantitative position. The limiting ring 122 is located below the breaking structure 121. When the sample adding piston 3 is in the quantitative position, the cover body 41 can abut against the limiting ring 122, and the sealing sleeve 33 can be sealed and matched with the quantitative area 113. By means of internal and external mechanical limiting, the accuracy of solution quantification can be further improved.

[0067] The present invention also discloses a detection device, which includes the above detection consumables 100. The specific structure of the detection consumables 100 refers to the above embodiment. Since the detection device adopts all the technical solutions of all the above embodiments, it at least has all the beneficial effects brought by the technical solutions of the above embodiments, which will not be repeated here. The detection device in this embodiment is mainly used for nucleic acid detection, and the reaction tube 2 can be a PCR reaction tube.

[0068] The bottom of the limiting ring 122 in this embodiment is connected to a reaction tube 2, and the cell preservation solution can be pre-placed in the sample area 111, and the detection reagent can be pre-placed in the detection cavity 21, so that the cell preservation solution and the detection reagent are placed in isolation. During the experiment, the split sealing cover 4 is first opened, and the sampling member 200 is placed in the sample area 111 containing the cell preservation solution, and then the split sealing cover 4 is covered. At this time, the pressure column 42 inside the split sealing cover 4 contacts one end of the sample adding piston 3 set in the piston area 112, as shown in FIG. Figure 7 As shown; by pressing down the split sealing cover 4, the breaking structure 121 of the side wall of the sample tube 1 is pressed and broken. During the downward movement of the split sealing cover 4 relative to the sample tube 1, the pressure column 42 pushes the sample adding piston 3 to move downward. Before the piercing tip 32 at the other end of the sample adding piston 3 passes through the sealing film 22, the sealing sleeve 33 at the lower part of the sample adding piston 3 isolates the detection chamber 21. The sealed space formed by the sealing sleeve 33 and the bottom of the sample tube 1 is quantitative, which limits the volume of the mixed solution of the sampling piece 200 and the cell preservation solution; continue to press down the split sealing cover 4, the piercing tip 32 pierces the sealing film 22, and then the limited volume of the mixed solution of the sampling piece 200 and the cell preservation solution is injected into the reaction tube 2, which is convenient for further detection of the quantitative volume of the mixed solution. The whole process is carried out in a sealed environment 15, which reduces the risk of contamination of reagents and samples, improves the accuracy of detection, and at the same time simplifies the operation process and improves the experimental efficiency.

[0069] In one embodiment, a sealing ring 15 is provided on the outer ring of the sample tube 1. The sealing ring 15 is provided near the opening 14 and is used to seal with the cover 41. The sealing ring 15 in this embodiment can be integrally formed with the main tube 12. The sealing ring 15 can be made of a sealing soft rubber component. The sealing ring 15 can improve the sealing stability between the cover 41 and the sample tube 1. During the continuous downward pressure of the cover 41, the sealing ring 15 can always maintain a sealing fit with the cover 41, thereby ensuring a closed environment in the sample tube 1 and the reaction tube 2. To facilitate the installation of the sealing ring 15, a stepped groove for the sealing ring 15 to extend into can be provided at the top of the sample tube 1.

[0070] like Figure 7 As shown, the avoidance cavity 411 includes a sealing section 4111, a transition section 4112 and an avoidance section 4113 connected in sequence, the sealing section 4111 is used to seal with the sealing ring 15, the inner diameter of the avoidance section 4113 is larger than the inner diameter of the sealing section 4111 and is used to avoid the sealing ring 15, and the inner diameter of the transition section 4112 gradually decreases from the avoidance section 4113 to the sealing section 4111. The sealing ring 15 is located above the breaking structure 121, the sealing section 4111 can be interference-fitted with the sealing ring 15, and the avoidance section 4113 and the transition section 4112 can facilitate the insertion of the sealing ring 15, thereby improving the assembly convenience between the split sealing cover 4 and the sample tube 1.

[0071] It should be noted that the pressure column 42 includes a column 421 and a plurality of reinforcing edges 422. The length of the reinforcing edges 422 is consistent with the length of the column 421, and the plurality of reinforcing edges 422 are connected to the outer peripheral wall of the column 421 at intervals around the circumference of the column 421. Figure 6 As shown, the outer periphery of the column 421 has four spaced reinforcing edges 422 , and any two adjacent reinforcing edges 422 form an angle of 90 degrees, and the reinforcing edges 422 are in contact with the inner tube wall of the sample tube 1 , which can realize the movement guidance of the pressure column 42 , thereby improving the operating convenience of the detection consumables 100 .

[0072] Specifically, the reinforcing rib 422 includes a connecting bend 4221 and a rib body 4222 extending from the connecting bend 4221 toward the sample tube 1. The column 421 and the connecting bend 4221 are both connected to the cover 41, and the cross-sectional size of the connecting bend 4221 is larger than the cross-sectional size of the rib body 4222. In this embodiment, the bottom of the reinforcing rib 422 is provided with a connecting bend 4221, which can increase the connection area between the reinforcing rib 422 and the cover 41. In this embodiment, the connecting bend 4221 can be provided with a triangular support bend, which can ensure the structural strength of the reinforcing rib 422 and the sample adding piston 3 during the pressing process.

[0073] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0074] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0075] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0076] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A detection consumable, characterized in that: The detection consumable (100) comprises: A sample tube (1) provided with a cavity (11) for containing a sample liquid; A reaction tube (2) connected to the sample tube (1), wherein the reaction tube (2) is provided with a detection cavity (21) for accommodating a detection reagent and a sealing film (22) for sealing the detection cavity (21); A sample loading piston (3) is accommodated in the cavity (11). The sample loading piston (3) can move relative to the sealing film (22) from an initial position to a sample loading position and a quantitative position in sequence. The sample loading piston (3) and the sealing film (22) at the initial position are arranged at intervals, so that the detection cavity (21) and the cavity (11) are isolated from each other. The sample loading piston (3) and the sealing film (22) at the sample loading position are in contact with each other and do not penetrate the sealing film (22). The sample loading piston (3) and the sealing film (22) at the quantitative position are limitedly matched and close the detection cavity (21). In addition, during the movement of the sample loading piston (3) between the sample loading position and the quantitative position, the detection cavity (21) is connected to the cavity (11), and the sample liquid can be quantitatively introduced into the detection cavity (21).

2. The detection consumable according to claim 1, characterized in that: The cavity (11) comprises: A sample area (111), used for containing a sample liquid; A piston area (112) is arranged side by side with the sample area (111) and is connected via a liquid inlet, and the sample adding piston (3) located at the initial position is accommodated in the piston area (112); The quantitative area (113) is located between the piston area (112) and the detection chamber (21). The quantitative area (113) is used to seal and cooperate with the sample adding piston (3) at the quantitative position and to seal the detection chamber (21).

3. The detection consumable according to claim 2, characterized in that: The sample adding piston (3) comprises: A piston rod (31) is provided with a piercing tip (32) at one end close to the reaction tube (2), and the piercing tip (32) is used to pierce the sealing film (22); A sealing sleeve (33) is sleeved on the outside of the piston rod (31) and is used for sealingly cooperating with the quantitative area (113).

4. The detection consumable according to claim 2, characterized in that: The sample tube (1) comprises: A main body tube (12), wherein the sample area (111) and the piston area (112) are both arranged in the main body tube (12); A connecting tube (13) is connected to the main tube (12) and the cross-sectional dimension of the connecting tube (13) is smaller than the cross-sectional dimension of the main tube (12); the quantitative zone (113) is arranged in the connecting tube (13); the reaction tube (2) is provided with an installation cavity (23) for the connecting tube (13) to extend into; the sealing film (22) is located between the installation cavity (23) and the detection cavity (21); and one end of the connecting tube (13) away from the main tube (12) abuts against the sealing film (22).

5. The detection consumable according to claim 2, characterized in that: The liquid inlet penetrates the piston area (112) along the length direction of the piston area (112), and the length of the sample adding piston (3) is smaller than the length of the liquid inlet.

6. The detection consumable according to any one of claims 1 to 5, characterized in that: An opening (14) is provided at one end of the cavity (11) away from the detection cavity (21), and the detection consumable (100) further comprises a split sealing cover (4) for opening or closing the opening (14).

7. The detection consumable according to claim 6, characterized in that: The split sealing cover (4) comprises: A cover body (41) is detachably connected to the sample tube (1), and the cover body (41) is provided with an avoidance cavity (411) for the sample tube (1) to extend into; A pressing column (42) is accommodated in the avoidance cavity (411) and connected to the cover body (41), and the pressing column (42) is used to press the sample adding piston (3) in a direction close to the detection cavity (21).

8. The detection consumable according to claim 7, characterized in that: The outer wall of the sample tube (1) is provided with a breaking structure (121) for abutting against the cover body (41), and the cover body (41) is used to break the breaking structure (121) and separate the breaking structure (121) from the sample tube (1) when the pressure column (42) presses against the sample adding piston (3).

9. The detection consumable according to claim 6, characterized in that: The outer wall of the sample tube (1) is further provided with a limiting ring (122), and the limiting ring (122) is used to limit the cover body (41) in the quantitative position.

10. A detection device, characterized in that: The detection device comprises the detection consumable (100) according to any one of claims 1 to 9.

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