A flow cytometer with improved particle resolution and methods of use thereof

Abstract

This invention belongs to the field of cell detection, specifically a flow cytometer with improved particle resolution and its usage method. It includes a protective shell, a first lens, a second lens, and a filter. A partition plate is welded and fixed inside the protective shell. An adjustable flow chamber is installed inside the protective shell. A homogenization assembly is installed inside the protective shell. A second hydraulic rod is installed at the top of the protective shell, and a mounting plate is fixedly connected to the bottom of the second hydraulic rod. A first through hole is formed on the mounting plate. Positioning blocks are fixedly connected to the top of the first lens, the second lens, and the filter. A laser is fixedly mounted on the side surface of the partition plate. A self-cleaning assembly is installed inside the protective shell. This invention solves the problems of existing flow cytometers not being able to conveniently adjust the diameter of the flow channel according to different types of samples, and not being able to automatically clean the various optical filters used.

Description

Technical Field

[0001] This invention relates to the field of cell detection, specifically to a flow cytometer that can improve particle resolution and its usage method. Background Technology

[0002] Flow cytometry is a technique for cell analysis, widely used in biomedical research, clinical diagnostics, and biopharmaceuticals. It works by suspending single cells in a liquid, irradiating them with lasers or other light sources, and analyzing their characteristics using photodetectors. Flow cytometry is a powerful tool that helps researchers and clinicians gain a deeper understanding of cell characteristics and functions, and it is of great significance in both basic research and clinical applications.

[0003] Existing flow cytometers have some problems in actual operation. For example, a flow cytometer with publication number CN116698710A, although capable of adjusting the internal temperature and humidity environment of the device, cannot easily adjust the diameter of the flow channel according to different types of samples (such as cell suspensions, particles, etc.) during actual operation. This affects the flow characteristics of the sample, resulting in low particle resolution and inaccurate detection results. Furthermore, it cannot automatically clean the various optical filters used during actual operation, nor can it easily replace suitable optical filters, resulting in poor signal acquisition and affecting the accuracy of the data. Therefore, there is a need to provide a flow cytometer with improved particle resolution and its usage method to meet the needs of users. Summary of the Invention

[0004] In view of the problems existing in current flow cytometers and their usage methods that can improve particle resolution, the present invention is proposed.

[0005] To solve the above-mentioned technical problems, according to one aspect of the present invention, the present invention provides the following technical solution: a flow cytometer with improved particle resolution, comprising a protective shell, a first lens, a second lens, and a filter; a partition plate is welded and fixed inside the protective shell; a first door plate and a second door plate are installed on the protective shell; a first hydraulic rod is installed at the bottom inside the protective shell; a placement frame is fixedly connected to the top of the first hydraulic rod; a test tube is placed inside the placement frame; an adjustable flow chamber is installed inside the protective shell; a homogenization assembly is installed inside the protective shell; support plates are welded and fixed to the inner wall of the protective shell and the side end faces of the partition plate; and a second hydraulic rod is installed at the top inside the protective shell. A mounting plate is fixedly connected to the bottom end of the second hydraulic rod. A first through hole is provided on the mounting plate. Positioning blocks are fixedly connected to the top ends of the first lens, the second lens, and the filter. A laser is fixedly mounted on the side end face of the partition plate. A first detector and a second detector are fixedly mounted on the inner wall of the protective shell. A self-cleaning assembly is installed inside the protective shell. A collection box is slidably connected to the bottom side end of the protective shell. A magnetic plate is fixedly connected to the side end face of the collection box. The magnetic plate is magnetically attracted to the bottom side end face of the protective shell. A second sponge frame is provided inside the collection box. A sponge board is fixedly connected inside the second sponge frame. A control panel is fixedly mounted on the side end face of the protective shell.

[0006] In a preferred embodiment of the present invention, the outer wall of the test tube is fitted with the inner wall of the placement frame, the top surface of the tray is fitted with the bottom surface of the mounting plate, four second hydraulic rods are provided, the four second hydraulic rods are evenly distributed around the mounting plate, the second lenses are symmetrically distributed on the front and rear sides inside the protective shell, the filters are equidistantly distributed on the front and rear sides inside the protective shell, the filters are inclined, the filters correspond one-to-one with the second detectors, the collection box is fitted with the inner wall of the protective shell, the inner wall of the collection box is fitted with the outer wall of the second sponge frame, and the sponge boards are equidistantly distributed inside the second sponge frame.

[0007] In a preferred embodiment of the present invention, the adjustable flow chamber includes a support plate, which is welded and fixed to the side end face of a partition plate. A first flow guide frame is welded and fixed to the support plate. A fixed frame is welded and fixed to the bottom of the first flow guide frame. A threaded rod is threadedly connected to the top of the fixed frame. A connecting plate is rotatably connected to the bottom end of the threaded rod. A push rod is hinged to the bottom end face of the connecting plate. A first flow guide plate is hinged to the bottom end of the push rod. A telescopic sleeve rod is welded and fixed to the side end face of the first flow guide plate. The other end of the telescopic sleeve rod is welded and fixed to the inner wall of the fixed frame. A first rubber block and a first rubber plate are fixedly connected to the bottom of the first flow guide frame. The bottom end of the first rubber block is fixedly connected to a second guide plate, the bottom end of the second guide plate is fixedly connected to a second rubber block, and the bottom end of the second rubber block is fixedly connected to the top end of the first guide plate. The side end faces of the second guide plate and the first guide plate are both fixed to the side end faces of the first rubber plate. There are four first guide plates and four first rubber plates, which are distributed alternately. The first guide plate corresponds one-to-one with the second rubber block, the second guide plate, the first rubber block, and the telescopic sleeve rod. The first guide plate corresponds one-to-one with the threaded rod through the connecting plate on the push rod. The outer diameter of the fixed frame is smaller than the diameter of the first through hole.

[0008] In a preferred embodiment of the present invention, a second guide frame is fixedly connected to the inner top surface of the first guide frame, a third rubber block and a second rubber plate are fixedly connected to the bottom end of the second guide frame, a third guide plate is fixedly connected to the bottom end of the third rubber block, a fourth rubber block is fixedly connected to the bottom end of the third guide plate, and a fourth guide plate is fixedly connected to the bottom end of the fourth rubber block. The side end faces of the third and fourth guide plates are both fixed to the side end faces of the second rubber plate. A fixing rod is fixedly connected to the inner wall of the first guide plate, and the other end of the fixing rod is fixedly connected to the outer wall of the fourth guide plate. Four fourth guide plates and four second rubber plates are provided, and the four fourth guide plates and four second rubber plates are distributed alternately. The fourth guide plate corresponds one-to-one with the fixing rod, the fourth rubber block, the third guide plate, and the third rubber block. The vertical center line of the first guide frame and the vertical center line of the second guide frame are located on the same vertical line.

[0009] In a preferred embodiment of the present invention, a first liquid pump is fixedly installed on the top side of the first flow guide frame, a first connecting pipe is connected to the flange of the first liquid pump, one end of the first connecting pipe is connected to the top of the second flow guide frame, and the other end of the first connecting pipe is located directly above the placement frame. A second liquid pump is fixedly installed on the inner top surface of the protective shell, a second connecting pipe is connected to the flange of the second liquid pump, one end of the second connecting pipe is connected to a flow divider frame, the flow divider frame is welded and fixed to the inner top surface of the first flow guide frame, a flow guide hole is provided through the bottom of the flow divider frame, the flow divider frame is generally annular, and the flow guide holes are distributed at equal angles at the bottom of the flow divider frame.

[0010] In a preferred embodiment of the present invention, the uniform processing component includes a first storage tank, which is welded and fixed to the inner bottom surface of a protective shell. A first inlet pipe is connected to the top of the first storage tank, and a first solenoid valve is installed on the first inlet pipe. A servo motor is installed and fixed to the top surface of the first storage tank. A first bevel gear is welded and fixed to the output shaft of the servo motor. A second bevel gear meshes with the first bevel gear. A sleeve is welded and fixed to the second bevel gear. The sleeve is rotatably connected to the middle part inside the first storage tank. The top of the sleeve is rotatably connected to the other end of a second connecting pipe via a sealed bearing. A stirring blade is welded and fixed to the sleeve. A fixing pipe is welded and fixed to the side surface of the stirring blade. A first conveying cylinder is fixedly connected to the bottom end of the fixing pipe. A first spiral rod is rotatably connected inside the first conveying cylinder. A circular gear is welded and fixed to one end of the first spiral rod. Tooth blocks are welded at equal angles to the inner bottom surface of the first storage tank, and the tooth blocks mesh with the circular gear. The first conveying cylinders are distributed at equal angles inside the first storage tank, and each first conveying cylinder corresponds to a fixing pipe.

[0011] In a preferred embodiment of the present invention, the positioning block has a slot on its side end, a positioning frame is welded and fixed to the bottom end surface of the mounting plate, a return spring is welded and fixed to the side end surface of the positioning frame, a locking rod is welded and fixed to the other end of the return spring, the locking rod is slidably connected in the positioning frame, the end of the locking rod is engaged in the slot, the slots are symmetrically distributed on both sides of the positioning block, the slots correspond one-to-one with the locking rods, and the positioning block is slidably connected in the positioning frame.

[0012] In a preferred embodiment of the present invention, the self-cleaning component includes a fixing plate, which is welded and fixed to the inner wall of the protective shell. A second through hole is provided through the fixing plate. A first sponge frame is fixedly connected to the fixing plate. A first conveying pipe, a second conveying pipe, and a third conveying pipe are fixedly connected inside the fixing plate. One end of the first conveying pipe is connected to the first sponge frame. The other end of the first conveying pipe and one end of the third conveying pipe are both connected to the second conveying pipe. The other end of the third conveying pipe is connected to a second conveying cylinder. The second conveying cylinder is fixedly connected inside a second storage box. The second storage box is welded and fixed to the side end face of the protective shell. A second spiral rod is rotatably connected inside the second conveying cylinder. The length and thickness of the first sponge frame are greater than the diameter and thickness of the first lens, respectively. The diameters of the first lens, the second lens, and the filter are the same. The length of the second spiral rod is greater than the length of the second conveying cylinder. The second conveying cylinder is fixed in the middle part inside the second storage box.

[0013] In a preferred embodiment of the present invention, a coil is fixedly connected to the top end of the second spiral rod, a traction rope is wound around the coil, the traction rope is slidably connected through the top side end of the second storage box and the top side end of the protective shell, the end of the traction rope is fixedly connected to the top surface of the mounting plate, a guide wheel is installed on the inner wall of the protective shell, the traction rope is slidably connected to the guide wheel, a second liquid inlet pipe is connected to the top side end of the second storage box, a second solenoid valve is installed on the second liquid inlet pipe, a limit frame is welded and fixed to the inner bottom surface of the second storage box, a spiral spring is welded and fixed to the inner wall of the limit frame, and the inner end of the spiral spring is welded and fixed to the bottom of the second spiral rod.

[0014] A method for using a flow cytometer that can improve particle resolution includes the following steps:

[0015] S1: Take samples from cells, tissues or organisms, prepare a single-cell suspension, place it in a test tube, and then place the test tube in the placement frame inside the protective shell;

[0016] S2: Before use, the first lens, the second lens, and each filter are automatically cleaned using a self-cleaning assembly, and the sheath fluid to be used is uniformly stirred by a uniform processing assembly.

[0017] S3: An adjustable flow chamber is used to aspirate and transport cell suspension and sheath fluid, and to guide the cell suspension through a gradually narrowing channel so that the cell population forms individual cells before passing the laser at the monitoring point;

[0018] S4: Each cell in the cell suspension moves on the laser beam emitted by the laser. With the help of the first lens and the first detector, each cell in the sample is analyzed individually. When each cell passes through the laser beam, the laser beam will scatter in multiple directions. With the help of various filters and corresponding second detectors, the laser beams scattered in multiple directions are collected and detected.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. This invention features an adjustable flow chamber. By rotating four threaded rods, the corresponding first guide plate can be pushed to move simultaneously towards the center or side via a pusher on the connecting plate. At this time, the first guide plate can push the corresponding fourth guide plate to move synchronously via a fixed rod. In conjunction with the second and third guide plates, the diameter of the flow channel can be conveniently adjusted according to the size of the cells. Adjusting the diameter of the flow channel can help optimize the flow rate to suit the analysis needs of cells, reduce cell aggregation, and ensure that cells pass through the analysis area in a single form. This can effectively improve the resolution of flow cytometer in particle analysis, and a suitable flow channel diameter can reduce bubble formation and avoid interference with the signal.

[0021] 2. This invention includes a uniform processing component. Driven by a servo motor, the first and second bevel gears drive the sleeve to rotate stably. Simultaneously, the sleeve, through the stirring blades, drives the first conveying cylinders on each fixed tube to rotate in a circular motion. At the same time, the meshing between the circular gears and the toothed blocks causes the first spiral rod inside the first conveying cylinder to rotate automatically during its revolution, thus conveying the sheath fluid from the bottom of the first storage tank to the top. This cycle, combined with the continuous stirring of the stirring blades, efficiently and uniformly stirs the sheath fluid in the first storage tank, preventing stratification or sedimentation during long-term storage and avoiding uneven distribution of components within the sheath fluid. The uniform sheath fluid reduces viscosity and increases fluidity, making it easier for cells to pass through the flow cytometer, thereby improving the accuracy of subsequent cell analysis and increasing the versatility and stability of the flow cytometer.

[0022] 3. This invention includes a self-cleaning component. Before cell detection begins, the mounting plate can be driven to move up and down reciprocally using the second hydraulic rod, which in turn drives the first lens, second lens, and each filter to move synchronously. Simultaneously, the mounting plate pulls the traction rope, which drives the second screw rod to rotate automatically, thus automatically delivering the purified water in the second storage tank to each of the first sponge frames. At this time, the first sponge frames can automatically clean the first lens, second lens, and each filter, avoiding the problem that dust or impurities attached to the first lens, second lens, and each filter affect the light transmittance, causing signal attenuation or distortion, and thus affecting the accuracy of the data. This can further improve the resolution of the flow cytometer in particle analysis.

[0023] 4. The present invention is provided with a locking rod and a locking slot. By using the cooperation of the locking rod and the locking slot, combined with the positioning frame and the positioning block, the first lens, the second lens and each filter can be easily disassembled and installed. This ensures the convenience of subsequent replacement of the first lens, the second lens and each filter, and increases the versatility and convenience of the flow cytometer.

[0024] 5. The present invention is provided with a collection box, a second sponge frame and a sponge plate. The collection box can be used to collect the used sheath fluid and cell suspension. At the same time, with the cooperation of the second sponge frame and each sponge plate, splashing during the waste liquid collection process can be effectively avoided, which may cause internal equipment pollution or pollution of the surrounding environment. Furthermore, the magnetic properties of the magnetic plate can be used to easily disassemble and install the collection box, thereby ensuring the convenience of subsequent waste liquid transfer and treatment. Attached Figure Description

[0025] To more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and detailed embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0026] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention;

[0027] Figure 2 This is a schematic diagram of the connection structure between the second guide plate and the first rubber plate of the present invention;

[0028] Figure 3 This is a schematic diagram of the connection structure between the third guide plate and the second rubber plate of the present invention;

[0029] Figure 4 This is a schematic diagram of the connection structure between the collection box and the sponge frame of the present invention;

[0030] Figure 5 This is a schematic diagram of the connection structure between the sponge frame and the sponge board of the present invention;

[0031] Figure 6 This is a schematic diagram of the main cross-sectional structure of the protective shell of the present invention;

[0032] Figure 7 This is a side sectional view of the protective shell structure of the present invention;

[0033] Figure 8 This is a schematic diagram of the main cross-sectional structure of the first storage box of the present invention;

[0034] Figure 9 This is a schematic diagram of the main cross-sectional structure of the sleeve of the present invention;

[0035] Figure 10 This is a top-section schematic diagram of the first storage box of the present invention;

[0036] Figure 11 This is a schematic diagram of the main cross-sectional structure of the first flow guide frame of the present invention;

[0037] Figure 12 This is the present invention. Figure 11 Enlarged structural diagram at point A in the middle;

[0038] Figure 13 This is a schematic diagram of the push rod structure from below in this invention;

[0039] Figure 14 This is a top-section schematic diagram of the diversion frame structure of the present invention;

[0040] Figure 15 This is a schematic diagram of the main cross-sectional structure of the second storage box of the present invention;

[0041] Figure 16 This is a top view schematic diagram of the spiral spring structure of the present invention;

[0042] Figure 17 This is a schematic diagram of the main cross-sectional structure of the positioning block of the present invention;

[0043] Figure 18 This is a top-section schematic diagram of the protective shell structure of the present invention;

[0044] Figure 19 This is a bottom view of the mounting plate structure of the present invention;

[0045] Figure 20 This is a top view schematic diagram of the first sponge frame structure of the present invention.

[0046] In the diagram: 1. Protective outer shell; 2. Partition plate; 3. First door panel; 4. Second door panel; 5. First hydraulic rod; 6. Placement frame; 7. Test tube; 8. Adjustable flow chamber; 801. Support plate; 802. First guide frame; 803. Fixing frame; 804. Threaded rod; 805. Connecting plate; 806. Push rod; 807. First guide plate; 808. Telescopic sleeve rod; 809. First rubber block; 810. Second guide plate; 811. Second rubber block; 812. First rubber plate; 813. Second guide frame; 814. Third rubber block. 815. Third guide plate; 816. Fourth rubber block; 817. Fourth guide plate; 818. Second rubber plate; 819. Fixing rod; 820. First liquid pump; 821. First connecting pipe; 822. Second liquid pump; 823. Second connecting pipe; 824. Diverter frame; 825. Guide hole; 9. Uniform processing component; 901. First storage tank; 902. First inlet pipe; 903. First solenoid valve; 904. Servo motor; 905. First bevel gear; 906. Second bevel gear; 907. Sleeve; 908. 909. Stirring blade; 910. Fixed pipe; 911. First conveying cylinder; 912. First screw rod; 913. Circular gear; 914. Tooth block; 10. Support plate; 11. Second hydraulic rod; 12. Mounting plate; 13. First through hole; 14. First lens; 15. Second lens; 16. Filter; 17. Positioning block; 18. Slot; 19. Positioning frame; 20. Return spring; 21. Locking rod; 22. Laser; 23. First detector; 24. Second detector; 25. Self-cleaning assembly; 2501. Fixed plate; 2502. Second... 2503. Through hole; 2504. First sponge frame; 2505. First conveying pipe; 2506. Second conveying pipe; 2507. Third conveying pipe; 2508. Second conveying cylinder; 2509. Second spiral rod; 25000. Coil; 2510. Traction rope; 2511. Guide wheel; 2512. Second storage box; 2513. Second liquid inlet pipe; 2514. Second solenoid valve; 2515. Limiting frame; 2516. Spiral spring; 26. Collection box; 27. Magnetic plate; 28. Second sponge frame; 29. ​​Sponge board; 30. Control panel. Detailed Implementation

[0047] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0048] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0049] Secondly, the present invention is described in detail with reference to the schematic diagrams. When detailing the embodiments of the present invention, for ease of explanation, the cross-sectional views illustrating the device structure may be partially enlarged, not according to the usual scale. Furthermore, the schematic diagrams are merely examples and should not limit the scope of protection of the present invention. In addition, actual fabrication should include three-dimensional spatial dimensions of length, width, and depth.

[0050] Example

[0051] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0052] like Figure 1-20As shown, a flow cytometer with improved particle resolution includes a protective shell 1, a first lens 14, a second lens 15, and a filter 16. A partition plate 2 is welded and fixed inside the protective shell 1. A first door plate 3 and a second door plate 4 are installed on the protective shell 1. A first hydraulic rod 5 is installed at the bottom inside the protective shell 1, and a placement frame 6 is fixedly connected to the top of the first hydraulic rod 5. Test tubes 7 are placed inside the placement frame 6. An adjustable flow chamber 8 is installed inside the protective shell 1. A homogenization assembly 9 is installed inside the protective shell 1. Support plates 10 are welded and fixed to the inner wall of the protective shell 1 and the side surfaces of the partition plate 2. A [missing information - likely a device or component] is installed at the top inside the protective shell 1. The second hydraulic rod 11 has a mounting plate 12 fixedly connected to its bottom end. A first through hole 13 is provided on the mounting plate 12. Positioning blocks 17 are fixedly connected to the top ends of the first lens 14, the second lens 15, and the filter 16. A laser 22 is fixedly mounted on the side end face of the partition plate 2. A first detector 23 and a second detector 24 are fixedly mounted on the inner wall of the protective housing 1. A self-cleaning assembly 25 is installed inside the protective housing 1. A collection box 26 is slidably connected to the bottom side end of the protective housing 1. A magnetic plate 27 is fixedly connected to the side end face of the collection box 26 and is magnetically attracted to the bottom of the protective housing 1. On the side end face of the protective housing 1, a second sponge frame 28 is provided inside the collection box 26, and a sponge plate 29 is fixedly connected inside the second sponge frame 28. A control panel 30 is installed and fixed on the side end face of the protective housing 1. The diameter of the flow channel can be easily adjusted according to the size of the cells using the adjustable flow chamber 8. Adjusting the diameter of the flow channel can help optimize the flow rate to suit the analysis needs of cells, reduce cell aggregation, and ensure that cells pass through the analysis area in a single form. This can effectively improve the resolution of the flow cytometer in particle analysis. In addition, the homogenization component 9 can efficiently and uniformly agitate the sheath fluid to be used. This avoids the problem of layering or precipitation of the sheath fluid during long-term storage, which can lead to uneven distribution of the internal components of the sheath fluid. Using a uniform sheath fluid can reduce the viscosity of the liquid and improve its fluidity, making it easier for cells to pass through the flow cytometer, thereby improving the accuracy of subsequent cell analysis. In addition, the use of the self-cleaning component 25 can automatically clean the first lens 14, the second lens 15 and each filter 16, avoiding the problem that the attached dust or impurities affect the light transmittance, causing signal attenuation or distortion, and thus affecting the accuracy of the data. This can further improve the resolution of the flow cytometer in particle analysis.

[0053] In this embodiment, the outer wall of the test tube 7 is attached to the inner wall of the placement frame 6, the top surface of the tray 10 is attached to the bottom surface of the mounting plate 12, four second hydraulic rods 11 are provided, and the four second hydraulic rods 11 are evenly distributed around the mounting plate 12, the second lenses 15 are symmetrically distributed on the front and rear sides inside the protective shell 1, the filters 16 are equidistantly distributed on the front and rear sides inside the protective shell 1, the filters 16 are inclined, and the filters 16 correspond one-to-one with the second detectors 24, the collection box 26 is attached to the inner wall of the protective shell 1, the inner wall of the collection box 26 is attached to the outer wall of the second sponge frame 28, and the sponge plates 29 are equidistantly distributed inside the second sponge frame 28. The collection box 26 can be used to collect the waste liquid of the sheath fluid and cell suspension after use. At the same time, with the cooperation of the second sponge frame 28 and each sponge plate 29, splashing during the waste liquid collection process can be effectively avoided, which may cause internal pollution of the equipment or pollution of the surrounding environment.

[0054] In this embodiment, the adjustable flow chamber 8 includes a support plate 801, which is welded and fixed to the side end face of the partition plate 2. A first flow guide frame 802 is welded and fixed to the support plate 801. A fixed frame 803 is welded and fixed to the bottom of the first flow guide frame 802. A threaded rod 804 is threadedly connected to the top of the fixed frame 803. A connecting plate 805 is rotatably connected to the bottom end of the threaded rod 804. A push rod 806 is hinged to the bottom end face of the connecting plate 805. A first flow guide plate 807 is hinged to the bottom end of the push rod 806. A telescopic sleeve rod 808 is welded and fixed to the side end face of the first flow guide plate 807. The other end of the telescopic sleeve rod 808 is welded and fixed to the inner wall of the fixed frame 803. A first rubber block 809 and a first... are fixedly connected to the bottom of the first flow guide frame 802. A rubber plate 812 and a first rubber block 809 are fixedly connected to the bottom end of a second guide plate 810. A second rubber block 811 is fixedly connected to the bottom end of the second guide plate 810. The bottom end of the second rubber block 811 is fixedly connected to the top end of the first guide plate 807. The side faces of the second guide plate 810 and the first guide plate 807 are both fixed to the side faces of the first rubber plate 812. Four first guide plates 807 and four first rubber plates 812 are provided, with the four first rubber plates 812 and four first guide plates 807 distributed alternately. Each first guide plate 807 corresponds one-to-one with a second rubber block 811, a second guide plate 810, a first rubber block 809, and a telescopic sleeve 808. The first guide plate 807 is connected to a connecting plate 8 on a push rod 806. 05 corresponds one-to-one with threaded rod 804. The outer diameter of fixed frame 803 is smaller than the diameter of first through hole 13. Second guide frame 813 is fixedly connected to the inner top surface of first guide frame 802. Third rubber block 814 and second rubber plate 818 are fixedly connected to the bottom end of second guide frame 813. Third guide plate 815 is fixedly connected to the bottom end of third rubber block 814. Fourth rubber block 816 is fixedly connected to the bottom end of third guide plate 815. Fourth guide plate 817 is fixedly connected to the bottom end of fourth rubber block 816. The side end faces of third guide plate 815 and fourth guide plate 817 are both fixed to the side end face of second rubber plate 818. Fixed rod 819 is fixedly connected to the inner wall of first guide plate 807. The other side of fixed rod 819... The end is fixedly connected to the outer wall of the fourth guide plate 817. There are four fourth guide plates 817 and four second rubber plates 818, arranged alternately. Each fourth guide plate 817 corresponds to a fixed rod 819, a fourth rubber block 816, a third guide plate 815, and a third rubber block 814. The vertical center line of the first guide frame 802 and the vertical center line of the second guide frame 813 are on the same vertical line. By rotating the four threaded rods 804, the corresponding first guide plates 807 can be pushed simultaneously towards the center or side via the push rod 806 on the connecting plate 805. At this time, the first guide plates 807 can push the corresponding fourth guide plates 817 to move synchronously via the fixed rod 819.With the second guide plate 810 and the third guide plate 815, the diameter of the flow channel can be easily adjusted according to the cell size.

[0055] In this embodiment, a first liquid pump 820 is fixedly installed on the top side of the first guide frame 802. A first connecting pipe 821 is connected to the flange of the first liquid pump 820. One end of the first connecting pipe 821 is connected to the top of the second guide frame 813, and the other end of the first connecting pipe 821 is located directly above the placement frame 6. A second liquid pump 822 is fixedly installed on the inner top surface of the protective shell 1. A second connecting pipe 823 is connected to the flange of the second liquid pump 822. One end of the second connecting pipe 823 is connected to a diversion frame 824. The diversion frame 824 is welded and fixed to the inner top surface of the first guide frame 802. A diversion hole 825 is provided through the bottom of the diversion frame 824. The diversion frame 824 is generally annular, and the diversion holes 825 are distributed at equal angles at the bottom of the diversion frame 824. By utilizing the diversion holes 825 on the diversion frame 824, it can be ensured that the sheath fluid can be evenly distributed in the first guide frame 802 during the suction and transportation process, thereby ensuring the stability of the subsequent transportation and flow state of the sheath fluid.

[0056] In this embodiment, the uniform processing component 9 includes a first storage tank 901, which is welded and fixed to the inner bottom surface of the protective shell 1. A first inlet pipe 902 is connected to the top of the first storage tank 901, and a first solenoid valve 903 is installed on the first inlet pipe 902. A servo motor 904 is installed and fixed on the top surface of the first storage tank 901, and a first bevel gear 905 is welded and fixed to the output shaft of the servo motor 904. A second bevel gear 906 meshes with the first bevel gear 905. A sleeve 907 is welded and fixed to the upper part of the first storage tank 901. The sleeve 907 is rotatably connected to the middle part of the interior of the first storage tank 901. The top of the sleeve 907 is rotatably connected to the other end of the second connecting pipe 823 through a sealed bearing. A stirring blade 908 is welded and fixed to the sleeve 907. A fixing pipe 909 is welded and fixed to the side end face of the stirring blade 908. A first conveying cylinder 910 is fixedly connected to the bottom end of the fixing pipe 909. A first spiral rod 911 is rotatably connected inside the first conveying cylinder 910. A circular gear 912 is welded and fixed to one end of the first spiral rod 911. The first storage tank 901... Toothed blocks 913 are welded at equal angles to the bottom surface of the inner side of the storage tank 901. The toothed blocks 913 mesh with the circular gears 912. The first conveying cylinders 910 are distributed at equal angles inside the first storage tank 901. The first conveying cylinders 910 correspond one-to-one with the fixed tubes 909. Driven by the servo motor 904, the first bevel gear 905 and the second bevel gear 906 can drive the sleeve 907 to rotate stably. At this time, the sleeve 907 can drive the first conveying cylinders 910 on each fixed tube 909 to perform circular motion through the stirring blades 908. At the same time, the... The meshing between the circular gear 912 and the toothed block 913 drives the first spiral rod 911 inside the first conveying cylinder 910 to rotate automatically during its revolution, thereby conveying the sheath fluid at the bottom of the first storage tank 901 to the top. This cycle, combined with the continuous stirring of each stirring blade 908, enables efficient and uniform stirring of the sheath fluid in the first storage tank 901. The uniform sheath fluid reduces the viscosity of the liquid, increases its fluidity, and makes it easier for cells to pass through the flow cytometer, thereby improving the accuracy of subsequent cell analysis.

[0057] In this embodiment, a slot 18 is provided on the side end of the positioning block 17, and a positioning frame 19 is welded and fixed on the bottom surface of the mounting plate 12. A return spring 20 is welded and fixed on the side end of the positioning frame 19, and a locking rod 21 is welded and fixed on the other end of the return spring 20. The locking rod 21 is slidably connected in the positioning frame 19, and the end of the locking rod 21 is engaged in the slot 18. The slots 18 are symmetrically distributed on both sides of the positioning block 17, and the slots 18 correspond one-to-one with the locking rods 21. The positioning block 17 is slidably connected in the positioning frame 19. By utilizing the cooperation of the locking rods 21 and the slots 18, combined with the positioning frame 19 and the positioning block 17, the disassembly and installation of the first lens 14, the second lens 15 and each filter 16 can be easily completed, thereby ensuring the convenience of subsequent replacement of the first lens 14, the second lens 15 and each filter 16.

[0058] In this embodiment, the self-cleaning component 25 includes a fixing plate 2501, which is welded and fixed to the inner wall of the protective shell 1. A second through hole 2502 is provided through the fixing plate 2501. A first sponge frame 2503 is fixedly connected to the fixing plate 2501. A first conveying pipe 2504, a second conveying pipe 2505, and a third conveying pipe 2506 are fixedly connected inside the fixing plate 2501. One end of the first conveying pipe 2504 is connected to the first sponge frame 2503. The other end of the first conveying pipe 2504 and one end of the third conveying pipe 2506 are both connected to the second conveying pipe 2505. The other end of the third conveying pipe 2506 is connected to a second conveying cylinder. 2507, the second conveying cylinder 2507 is fixedly connected inside the second storage box 2512, which is welded and fixed to the side end face of the protective shell 1. A second spiral rod 2508 is rotatably connected inside the second conveying cylinder 2507. The length and thickness of the first sponge frame 2503 are greater than the diameter and thickness of the first lens 14, respectively. The diameters of the first lens 14, the second lens 15, and the filter 16 are the same. The length of the second spiral rod 2508 is greater than the length of the second conveying cylinder 2507. The second conveying cylinder 2507 is fixed in the middle part inside the second storage box 2512. A coil 2509 is fixedly connected to the top end of the second spiral rod 2508. A traction rope 2510 is wound around a coil 2509. The traction rope 2510 passes through and slides between the top side of the second storage box 2512 and the top side of the protective shell 1. The end of the traction rope 2510 is fixedly connected to the top surface of the mounting plate 12. A guide wheel 2511 is installed on the inner wall of the protective shell 1, and the traction rope 2510 is slidably connected to the guide wheel 2511. A second liquid inlet pipe 2513 is connected to the top side of the second storage box 2512, and a second solenoid valve 2514 is installed on the second liquid inlet pipe 2513. A limit frame 2515 is welded and fixed to the bottom surface inside the second storage box 2512, and a limit valve 2514 is welded and fixed to the inner wall of the limit frame 2515. The inner end of the spiral spring 2516 is welded and fixed to the bottom of the second spiral rod 2508. The second hydraulic rod 11 drives the mounting plate 12 to move up and down, thereby driving the first lens 14, the second lens 15 and each filter 16 to move synchronously. At the same time, the mounting plate 12 pulls the traction rope 2510, which drives the second spiral rod 2508 to rotate automatically, thereby automatically transporting the clean water in the second storage box 2512 to each first sponge frame 2503. At this time, the first sponge frames 2503 can automatically clean the first lens 14, the second lens 15 and each filter 16.

[0059] It should be noted that the present invention is a flow cytometer that can improve particle resolution and its usage method. First, the staff takes materials from cells, tissues or organisms, prepares a single-cell suspension, and then delivers it into test tube 7. At this time, the staff can open the first door panel 3 on the protective shell 1 and place the test tube 7 in the placement frame 6. Then, by driving the first hydraulic rod 5, the placement frame 6 can be pushed upward, which in turn can push the test tube 7 upward, so that the first connecting tube 821 can be stably inserted into the test tube 7.

[0060] Then, the operator can drive the servo motor 904 on the first storage box 901 and the second hydraulic rod 11 inside the protective shell 1. Under the drive of the servo motor 904, the first bevel gear 905 on the output shaft drives the second bevel gear 906 to rotate, which in turn drives the sleeve 907 to rotate stably inside the first storage box 901. At this time, under the continuous rotation of the sleeve 907, the stirring blade 908 can drive each fixed tube 909 to perform circular motion, which in turn drives the corresponding first conveying cylinder 910 of each fixed tube 909 to perform synchronous circular motion. During the movement of each first conveying cylinder 910, the circular gear 912 on the internal first spiral rod 911 can perform circular motion on the tooth block 913, that is, to revolve. During the revolution of the circular gear 912, through meshing with the tooth block 913, the circular gear 912 can be driven to rotate automatically, which in turn can drive the first screw rod 911 to rotate automatically inside the first conveying cylinder 910 during the revolution. At this time, under the continuous rotation of the first screw rod 911, the sheath fluid at the bottom of the first storage tank 901 can be sucked through the first conveying cylinder 910, and the sheath fluid at the bottom can be transported to the top with the help of the fixed pipe 909. By utilizing the circulation of the sheath fluid, combined with the continuous stirring of each stirring blade 908, the sheath fluid in the first storage tank 901 can be efficiently and uniformly stirred, avoiding the problem of uneven distribution of the internal components of the sheath fluid due to stratification or sedimentation during long-term storage.

[0061] Meanwhile, driven by the second hydraulic rod 11, the mounting plate 12 moves upward and then downward. When the mounting plate 12 moves downward, the limiting action of the support plate 10 ensures that the mounting plate 12 can easily and accurately reset, avoiding movement deviations that could affect the accuracy of subsequent testing. When the mounting plate 12 moves upward, it drives the first lens 14, the second lens 15, and each filter 16 to move upward synchronously until they reach above the first sponge frame 2503. Simultaneously, the movement of the mounting plate 12 drives the end of the traction rope 2510 to move upward synchronously. At this time, the spiral spring 2516 inside the limiting frame 2515... Under the elastic action, the second screw rod 2508 inside the second conveying cylinder 2507 can be driven to rotate automatically. At the same time, the second screw rod 2508 can automatically wind up the traction rope 2510 through the coil 2509 at the top, ensuring the stability of the traction rope 2510 in subsequent repeated work. At this time, under the automatic rotation of the second screw rod 2508, the clean water in the second storage box 2512 can be drawn and transported to the third conveying pipe 2506 through the second conveying cylinder 2507. Then, the clean water can be automatically transported to each of the first conveying pipes 2504 through the second conveying pipe 2505, and then transported to each of the first sponge frames 2503 to moisten the first sponge frames 2503.

[0062] Subsequently, the second hydraulic rod 11 pushes the mounting plate 12 downward to reset. Under the downward action of the mounting plate 12, the traction rope 2510 on the guide wheel 2511 is pulled. At this time, under the pulling action of the traction rope 2510, the second spiral rod 2508 can be driven to rotate in the opposite direction through the coil 2509. At this time, the spiral feeding direction of the second spiral rod 2508 is reversed, and it will not continue to deliver clean water. However, at this time, each of the first sponge frames 2503 has absorbed clean water, which is sufficient for use. At this time, under the action of the mounting plate 12, the first lens 14 that is locked in place can be driven to engage. The second lens 15 and each filter 16 move downwards synchronously to reset. During their movement, they can pass through each first sponge frame 2503. Under the action of each first sponge frame 2503, the dust and impurities attached to the end faces of the first lens 14, the second lens 15 and each filter 16 can be automatically cleaned. This prevents the dust or impurities attached to the first lens 14, the second lens 15 and each filter 16 from affecting the light transmittance, causing signal attenuation or distortion, and thus affecting the accuracy of the data. In this way, the resolution of the flow cytometer in particle analysis can be effectively improved.

[0063] Then, the operator can adjust the bottom flow diameter of the first guide frame 802 and the bottom flow diameter of the second guide frame 813 according to the cell size. The operator can rotate the four threaded rods 804 on the fixed frame 803. Under the rotation of the threaded rods 804, the connecting plate 805 can be pushed downward. At the same time, under the limiting and guiding action of the telescopic sleeve rod 808, each threaded rod 804 can push the corresponding first guide plate 807 to move towards the middle or side simultaneously through the push rod 806 on the connecting plate 805. Under the movement of each first guide plate 807, in conjunction with the first rubber block 809 and the second rubber block 811, the tilt angle of the second guide plate 810 can be adjusted. Adjustments are made, and under the movement of the first guide plate 807, in conjunction with the alternating first rubber plates 812, the bottom flow diameter of the first guide frame 802 can be adjusted; similarly, during the movement of each first guide plate 807, the corresponding fourth guide plate 817 can be pushed to move simultaneously to the center or side by the fixing rod 819. Under the movement of each fourth guide plate 817, in conjunction with the third rubber block 814 and the fourth rubber block 816, the tilt angle of the third guide plate 815 can be adjusted, and under the movement of each fourth guide plate 817, in conjunction with the alternating second rubber plates 818, the bottom flow diameter of the second guide frame 813 can be adjusted synchronously.

[0064] Then, the operator can drive the first liquid pump 820 and the second liquid pump 822. Driven by the first liquid pump 820, the cell suspension in the test tube 7 can be aspirated through the first connecting tube 821. Simultaneously, driven by the second liquid pump 822, the sheath fluid inside the first storage tank 901 can be aspirated through the second connecting tube 823 and the sleeve 907. Before aspirating the sheath fluid, the stirring of the sheath fluid can be stopped. At this time, under the aspiration and transport of the sheath fluid, the second connecting tube 823 can... The sheath fluid is delivered into the diversion frame 824, and then the diversion frame 824 can evenly deliver the fluid into the first diversion frame 802 through the various diversion holes 825 at the bottom. At the same time, under the suction and delivery action of the first connecting tube 821, the cell suspension can be delivered into the second diversion frame 813. At this time, under the synchronous flow of the cell suspension and the sheath fluid, the sheath fluid can create a laminar flow environment, reduce the friction between the cells and the inner wall of the flow channel, and allow the cells to pass through the detection area stably in a single form, avoiding aggregation and mutual interference.

[0065] As the cell suspension flows downwards, each individual cell inside the cell suspension passes through the laser beam generated by the laser 22. After being irradiated, the forward-scattered light passes through the first lens 14 and irradiates the first detector 23. Similarly, the side-scattered light passes through the second lenses 15 on both the front and rear sides and irradiates the filter 16, and is then reflected to the corresponding second detector 24. By collecting and detecting the side-scattered light from both the front and rear sides, the detection accuracy can be further improved. The collected light signals are converted into electrical signals, and after computer analysis, cell characteristic data such as number, size, and fluorescence intensity are generated.

[0066] After testing, the waste liquid can automatically fall into the collection box 26. The collection box 26 can be used to collect the used sheath fluid and cell suspension. At the same time, with the cooperation of the second sponge frame 28 and each sponge plate 29, splashing during the waste liquid collection process can effectively prevent internal equipment pollution or environmental pollution. After the testing is completed, the staff only needs to pull the magnetic plate 27 outward until the magnetic plate 27 moves away from the protective shell 1. At this time, the magnetic plate 27 can drive the collection box 26 to move away from the protective shell 1, thus ensuring the convenience of subsequent waste liquid transfer and treatment. The staff can also remove the second sponge frame 28 for easy cleaning. After cleaning, the collection box 26 can be stably inserted into the protective shell 1. The magnetic properties of the magnetic plate 27 can be used to easily complete the magnetic installation of the collection box 26, ensuring the stability of the collection box 26 in subsequent operation.

[0067] Furthermore, by opening the second door panel 4 on the protective casing 1, the operator can pull the lever 21 on the positioning frame 19 outwards until the lever 21 disengages from the slot 18 on the positioning block 17. At this point, the positioning block 17 can disengage from the positioning frame 19, thus facilitating the disassembly of the first lens 14, the second lens 15, or each filter 16. Similarly, when it is necessary to engage or disengage the first lens 14, the second lens 15, or each filter 16, simply pull the lever 21 on the positioning frame 19 outwards again. When inserting the positioning blocks 17 on the first lens 14, the second lens 15, or each filter 16 into the positioning frame 19, the operator can then release the locking lever 21. At this time, under the elastic action of the return spring 20, the locking lever 21 can be rotated and engaged into the slot 18 on the positioning block 17, thereby facilitating the engagement and installation of the first lens 14, the second lens 15, or each filter 16. By facilitating the replacement of the first lens 14, the second lens 15, or each filter 16, the service life of the flow cytometer can be effectively improved.

[0068] Although the present invention has been described above with reference to embodiments, various modifications can be made and components can be replaced with equivalents without departing from the scope of the invention. In particular, as long as there is no structural conflict, the features in the disclosed embodiments can be combined with each other in any manner. The lack of an exhaustive description of these combinations in this specification is merely for the sake of brevity and resource conservation. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A flow cytometer with improved particle resolution, comprising a protective shell (1), a first lens (14), a second lens (15), and a filter (16), characterized in that: A partition plate (2) is welded and fixed inside the protective shell (1). A first door plate (3) and a second door plate (4) are installed on the protective shell (1). A first hydraulic rod (5) is installed at the bottom inside the protective shell (1). A placement frame (6) is fixedly connected to the top of the first hydraulic rod (5). A test tube (7) is placed inside the placement frame (6). An adjustable flow chamber (8) is installed inside the protective shell (1). A uniform processing component (9) is installed inside the protective shell (1). A support plate (10) is welded and fixed to the inner wall of the protective shell (1) and the side end face of the partition plate (2). A second hydraulic rod (11) is installed at the top inside the protective shell (1). A support plate (10) is fixedly connected to the bottom of the second hydraulic rod (11). Mounting plate (12), through which a first through hole (13) is provided, and positioning blocks (17) are fixedly connected to the top of the first lens (14), the second lens (15) and the filter (16). A laser (22) is fixedly mounted on the side end face of the partition plate (2). A first detector (23) and a second detector (24) are fixedly mounted on the inner wall of the protective shell (1). A self-cleaning assembly (25) is installed inside the protective shell (1). A collection box (26) is slidably connected to the bottom side end of the protective shell (1). A magnetic plate (27) is fixedly connected to the side end face of the collection box (26). The magnetic plate (27) is magnetically attracted to the bottom side end face of the protective shell (1). Above, a second sponge frame (28) is provided inside the collection box (26), and a sponge board (29) is fixedly connected inside the second sponge frame (28). A control panel (30) is installed and fixed on the side end face of the protective shell (1). The adjustable flow chamber (8) includes a support plate (801). The support plate (801) is welded and fixed to the side end face of the partition plate (2). A first flow guide frame (802) is welded and fixed on the support plate (801). A fixed frame (803) is welded and fixed to the bottom of the first flow guide frame (802). A threaded rod (804) is threadedly connected to the top of the fixed frame (803). A connecting plate (805) is rotatably connected to the bottom end of the threaded rod (804). The connecting plate (805) is threaded and fixed to the bottom end of the threaded rod (804). 5) A push rod (806) is hinged to the bottom end of the push rod (806), and a first guide plate (807) is hinged to the bottom end of the push rod (806). A telescopic sleeve rod (808) is welded and fixed to the side end of the first guide plate (807). The other end of the telescopic sleeve rod (808) is welded and fixed to the inner wall of the fixed frame (803). A first rubber block (809) and a first rubber plate (812) are fixedly connected to the bottom of the first guide frame (802). A second guide plate (810) is fixedly connected to the bottom end of the first rubber block (809). A second rubber block (811) is fixedly connected to the bottom end of the second guide plate (810). The bottom end of the second rubber block (811) is fixedly connected to the top end of the first guide plate (807).The side end face of the second guide plate (810) and the side end face of the first guide plate (807) are both fixed to the side end face of the first rubber plate (812). There are four first guide plates (807) and four first rubber plates (812), which are distributed alternately with the four first guide plates (807). The first guide plates (807) correspond one-to-one with the second rubber block (811), the second guide plate (810), the first rubber block (809), and the telescopic sleeve (808). The first guide plate (807) corresponds one-to-one with the threaded rod (804) via the connecting plate (805) on the push rod (806). The outer diameter of the fixed frame (803) is smaller than the diameter of the first through hole (13). A second guide frame (813) is fixedly connected to the inner top surface of the first guide frame (802). A third rubber block (814) and a second rubber plate (818) are fixedly connected to the bottom end of the second guide frame (813). A third guide plate is fixedly connected to the bottom end of the third rubber block (814). (815), a fourth rubber block (816) is fixedly connected to the bottom end of the third guide plate (815), and a fourth guide plate (817) is fixedly connected to the bottom end of the fourth rubber block (816). The side end faces of the third guide plate (815) and the fourth guide plate (817) are both fixed to the side end faces of the second rubber plate (818). A fixing rod (819) is fixedly connected to the inner wall of the first guide plate (807), and the other end of the fixing rod (819) is fixedly connected to the fourth guide plate (818). On the outer wall of the first guide frame (802), four fourth guide plates (817) and four second rubber plates (818) are provided, with the four fourth guide plates (817) and four second rubber plates (818) distributed alternately. Each fourth guide plate (817) corresponds one-to-one with a fixed rod (819), a fourth rubber block (816), a third guide plate (815), and a third rubber block (814). The vertical center line of the first guide frame (802) and the vertical center line of the second guide frame (813) are located on the same vertical line.

2. The flow cytometer with improved particle resolution according to claim 1, characterized in that: The outer wall of the test tube (7) is in contact with the inner wall of the placement frame (6), the top surface of the tray (10) is in contact with the bottom surface of the mounting plate (12), four second hydraulic rods (11) are provided, and the four second hydraulic rods (11) are evenly distributed around the mounting plate (12), the second lens (15) is symmetrically distributed on the front and rear sides inside the protective shell (1), the filter (16) is equidistantly distributed on the front and rear sides inside the protective shell (1), the filter (16) is inclined, the filter (16) corresponds one-to-one with the second detector (24), the collection box (26) is in contact with the inner wall of the protective shell (1), the inner wall of the collection box (26) is in contact with the outer wall of the second sponge frame (28), and the sponge board (29) is equidistantly distributed inside the second sponge frame (28).

3. A flow cytometer with improved particle resolution according to claim 1, characterized in that: A first liquid pump (820) is fixedly installed on the top side of the first flow guide frame (802). A first connecting pipe (821) is connected to the flange of the first liquid pump (820). One end of the first connecting pipe (821) is connected to the top of the second flow guide frame (813). The other end of the first connecting pipe (821) is located directly above the placement frame (6). A second liquid pump (822) is fixedly installed on the inner top surface of the protective shell (1). A second connecting pipe (823) is connected to the flange of the second liquid pump (822). One end of the second connecting pipe (823) is connected to a diversion frame (824). The diversion frame (824) is welded and fixed to the inner top surface of the first flow guide frame (802). A flow guide hole (825) is opened through the bottom of the diversion frame (824). The diversion frame (824) is generally annular. The flow guide holes (825) are distributed at equal angles at the bottom of the diversion frame (824).

4. A flow cytometer with improved particle resolution according to claim 3, characterized in that: The uniform processing component (9) includes a first storage tank (901), which is welded and fixed to the inner bottom surface of the protective shell (1). A first liquid inlet pipe (902) is connected to the top of the first storage tank (901), and a first solenoid valve (903) is installed on the first liquid inlet pipe (902). A servo motor (904) is installed and fixed on the top surface of the first storage tank (901). A first bevel gear (905) is welded and fixed to the output shaft of the servo motor (904). A second bevel gear (906) is meshed with the first bevel gear (905). A sleeve (907) is welded and fixed to the second bevel gear (906). The sleeve (907) is rotatably connected to the middle part inside the first storage tank (901). The top of the sleeve (907) is... The other end of the second connecting pipe (823) is rotatably connected to the sealed bearing. A stirring blade (908) is welded and fixed on the sleeve (907). A fixing pipe (909) is welded and fixed on the side end face of the stirring blade (908). A first conveying cylinder (910) is fixedly connected to the bottom end of the fixing pipe (909). A first spiral rod (911) is rotatably connected inside the first conveying cylinder (910). A circular gear (912) is welded and fixed to one end of the first spiral rod (911). Tooth blocks (913) are welded at equal angles on the bottom surface inside the first storage box (901). The tooth blocks (913) mesh with the circular gear (912). The first conveying cylinders (910) are distributed at equal angles inside the first storage box (901). The first conveying cylinders (910) correspond one-to-one with the fixing pipes (909).

5. A flow cytometer with improved particle resolution according to claim 1, characterized in that: The positioning block (17) has a slot (18) on its side end. A positioning frame (19) is welded and fixed on the bottom surface of the mounting plate (12). A reset spring (20) is welded and fixed on the side surface of the positioning frame (19). A locking rod (21) is welded and fixed on the other end of the reset spring (20). The locking rod (21) is slidably connected in the positioning frame (19). The end of the locking rod (21) is engaged in the slot (18). The slots (18) are symmetrically distributed on both sides of the positioning block (17). The slots (18) correspond one-to-one with the locking rods (21). The positioning block (17) is slidably connected in the positioning frame (19).

6. A flow cytometer with improved particle resolution according to claim 1, characterized in that: The self-cleaning component (25) includes a fixing plate (2501), which is welded and fixed to the inner wall of the protective shell (1). A second through hole (2502) is provided through the fixing plate (2501). A first sponge frame (2503) is fixedly connected to the fixing plate (2501). A first conveying pipe (2504), a second conveying pipe (2505), and a third conveying pipe (2506) are fixedly connected inside the fixing plate (2501). One end of the first conveying pipe (2504) is connected to the first sponge frame (2503). The other end of the first conveying pipe (2504) and one end of the third conveying pipe (2506) are both connected to the second conveying pipe (2505). The third conveying pipe (2506)... The other end is connected to a second conveying cylinder (2507), which is fixedly connected inside the second storage box (2512). The second storage box (2512) is welded and fixed to the side end face of the protective shell (1). A second spiral rod (2508) is rotatably connected inside the second conveying cylinder (2507). The length and thickness of the first sponge frame (2503) are greater than the diameter and thickness of the first lens (14), respectively. The diameter of the first lens (14), the diameter of the second lens (15), and the diameter of the filter (16) are the same. The length of the second spiral rod (2508) is greater than the length of the second conveying cylinder (2507). The second conveying cylinder (2507) is fixed in the middle part inside the second storage box (2512).

7. A flow cytometer with improved particle resolution according to claim 6, characterized in that: A coil (2509) is fixedly connected to the top end of the second spiral rod (2508). A traction rope (2510) is wound around the coil (2509). The traction rope (2510) slides through and is connected to the top side of the second storage box (2512) and the top side of the protective shell (1). The end of the traction rope (2510) is fixedly connected to the top surface of the mounting plate (12). A guide wheel (2511) is installed on the inner wall of the protective shell (1). The traction rope (2510) slides through and is connected to the top surface of the mounting plate (12). On the guide wheel (2511), a second liquid inlet pipe (2513) is connected to the top side of the second storage box (2512). A second solenoid valve (2514) is installed on the second liquid inlet pipe (2513). A limit frame (2515) is welded and fixed on the inner bottom surface of the second storage box (2512). A spiral spring (2516) is welded and fixed on the inner wall of the limit frame (2515). The inner end of the spiral spring (2516) is welded and fixed to the bottom of the second spiral rod (2508).

8. A method of using a flow cytometer with improved particle resolution, comprising the flow cytometer with improved particle resolution as described in claim 1, characterized in that, Includes the following steps: S1: Take samples from cells, tissues or organisms, prepare a single-cell suspension, and place it in a test tube (7). Then place the test tube (7) in the placement frame (6) in the protective shell (1). S2: Before use, the first lens (14), the second lens (15) and each filter (16) are automatically cleaned by the self-cleaning assembly (25), and the sheath liquid to be used is uniformly stirred by the uniform processing assembly (9). S3: The cell suspension and sheath fluid are pumped and transported using an adjustable flow chamber (8), and the cell suspension is guided through a gradually narrowing channel so that the cell population forms individual cells before passing the laser (22) at the monitoring point; S4: Each cell in the cell suspension moves on the laser beam emitted by the laser (22). With the help of the first lens (14) and the first detector (23), each cell in the sample is analyzed individually. When each cell passes through the laser beam, the laser beam will scatter in multiple directions. With the help of each filter (16) and the corresponding second detector (24), the laser beam scattered in multiple directions is collected and detected.

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