Pomegranate fermentation product with epigenetic action mechanism

Abstract

The invention relates to a pomegranate leavening with an epigenetic action mechanism, which is characterized by being prepared by the following method: A, preparing pomegranate pulp, and pasteurizing at 68-70 DEG C for 30-40 minutes; b, adding 3-6 g / L of mixed lactobacillus, 10-20 g / L of casein and 25-50 g / L of fructose into the pomegranate pulp, and carrying out fermentation treatment; c, filtering to obtain pomegranate fruit fermentation liquor; d, performing vacuum freeze-drying on the pomegranate fruit fermentation liquor by adopting a vacuum freeze-drying machine; e, preparing a refined pomegranate fruit leavening dry basis; and F, mixing the dry basis of the pomegranate fruit fermentation product with maltodextrin, and performing spray drying to obtain the pomegranate fermentation product with the epigenetic action mechanism. The pomegranate leavening with the epigenetic action mechanism has the effect of inhibiting DNA methylation and has the beneficial effect of improving CDK1 gene expression.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a pomegranate fermentation product with an epigenetic action mechanism. Background Art

[0002] Epigenetics refers to changes in gene or protein expression that do not involve changes in DNA sequences and can be stably transmitted during development and cell proliferation. The main content of epigenetics is divided into two parts: regulation of gene transcription and regulation after gene transcription. Epigenetic regulation and modification are involved in a series of important processes in life, including development, hematopoiesis, organ formation, apoptosis and cell proliferation, aging, etc. In recent years, epigenetic research has mainly focused on DNA methylation, histone modification, microRNA regulation, etc.

[0003] DNA methylation is an important epigenetic modification that plays a key role in regulating gene expression and cell function. DNA methylation refers to the transfer of a methyl group of S-adenosylmethionine (SAM) to the 5th carbon atom of cytosine under the action of DNA methyltransferases (DNMTs: including DNMT1, DNMT2, DNMT3a, DNMT3b) to form 5-methylcytosine (5mc). Usually, high methylation inhibits gene expression, low methylation promotes gene expression, and the methylation state of DNA can be reversed at a certain stage of biological development or a certain state of cell differentiation. With age, the DNA methylation pattern in skin cells changes. These changes are closely related to abnormal gene expression and decreased cell function, which ultimately lead to the characteristics of skin aging.

[0004] DNA methyltransferase 1 (DNMT1) is a key enzyme that is responsible for transferring methylation marks from the original DNA chain to the newly synthesized DNA chain when DNA is replicated during cell division, thereby maintaining the cell's epigenetic memory. Studies have found that moderate inhibition of DNMT1 activity may help restore the original methylation state of cells, thereby promoting cell regeneration and repair and combating aging.

[0005] DNA methylation is the earliest discovered epigenetic mechanism and is also the most studied and understood mechanism. In the past, the application of epigenetics was mostly focused on pathological research fields such as disease treatment and new drug development. In recent years, epigenetics has begun to attract attention in the study of skin anti-aging mechanisms, but there are few research reports on cosmetic active ingredients that inhibit the mechanism of DNA methylation.

[0006] Histone modification is another important direction of epigenetic research, including acetylation, methylation, phosphorylation and ribosylation. Studies have shown that CDK1 phosphorylates proteins involved in epigenetic regulation, which can affect the expression of other genes through the mechanism of epigenetic modification and has the function of maintaining epigenetic characteristics. As a kinase, CDK1 participates in the process of regulating the cell cycle. The normal cell cycle includes cell division, differentiation, apoptosis or pyroptosis, which can be regulated by a variety of regulatory proteins. Epigenetic mechanisms such as DNA methylation of specific genes or the entire genome, histone modification and the action of non-coding RNAs (ncRNAs) play an important role in the cell cycle process. Changes in these mechanisms cause cell cycle dysregulation, leading to cell growth disorders and abnormal epigenetic modifications. The key to cell cycle arrest is the decrease in the number and activity of CDK1 and CDK4, so activating CDK1 and CDK4 helps restart the orderly cell cycle and restore epigenetic effects.

[0007] However, there are few research reports on natural plant fermentation raw materials for cosmetics that can inhibit DNA methylation, activate CDK1 gene expression, and restore epigenetic effects. Summary of the invention

[0008] The technical problem to be solved by the present invention is to provide a pomegranate fermentation product which can inhibit DNA methylation, enhance CDK1 gene expression and has an epigenetic mechanism.

[0009] In order to solve the above technical problems, the present invention provides a pomegranate fermentation product with an epigenetic mechanism, which is characterized by being prepared by the following method: A. Peel fresh ripe pomegranates, weigh 300-500 g of pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 68-70°C for 30-40 min; B. Add 3-6 g / L mixed lactobacillus, 10-20 g / L casein and 25-50 g / L fructose to the pomegranate pulp described in step A, adjust the pH of the fermentation broth to 6-8 using L-arginine, and culture for 48-72 h at 36.5-37.7 ° C. During the culture, the urolithin A content in the fermentation broth was tested by high performance liquid chromatography, and the fermentation end point was 12 g / L of urolithin A; the mixed lactobacillus was mixed by Lactobacillus reuteri ATCC PTA6475, Lactobacillus ATCC 53103, and Lactobacillus plantarum ATCC 8014 in a mass ratio of 1:1:1; C. Filter using a 300-mesh filter cloth to obtain pomegranate fruit fermentation liquid; D. Using a vacuum freeze dryer, adjusting the temperature to -80~-60°C, vacuum freeze drying the pomegranate fruit fermentation liquid for 48 h to obtain a fermentation dry basis; E. The fermented product dry basis and 85% ethanol by volume concentration are mixed and dissolved in a mass ratio of 1:5-20, and a 300-mesh filter cloth is used to filter and remove the alcohol-insoluble matter. The alcohol-soluble matter is freeze-dried again using a vacuum freeze dryer at a freeze-drying temperature of -80 to -60 ° C, and the ethanol and water are removed to obtain a refined pomegranate fruit fermented product dry basis; F. Weigh the dry basis of the pomegranate fruit fermentation product obtained in step E and maltodextrin at a mass ratio of 1:4-10, mechanically mix them evenly to obtain a premix, mix and dissolve the premix with deionized water at a mass ratio of 1:50, spray dry after complete dissolution, and spray dry under the conditions of: inlet air temperature of 120-160° C., outlet temperature of 60-90° C., feed rate of 300-400 ml / h, spray working pressure of 0.3-0.5 MPa, air volume of 2.5 cubic meters / minute, and control the moisture content of the spray-dried powder to be no higher than 3%. The obtained spray-dried powder is the pomegranate fermentation product with an epigenetic action mechanism.

[0010] The application of the pomegranate fermentation product with epigenetic mechanism in cosmetics can inhibit DNA methylation and enhance CDK1 gene expression.

[0011] The casein described in the present invention has a CAS number of 9000-71-9, is derived from milk, and has a purity of ≥90%.

[0012] The fructose of the present invention has a CAS number of 57-48-7 and a purity of ≥90%.

[0013] The L-arginine described in the present invention has a CAS number of 74-79-3 and is analytically pure; the maltodextrin described in the present invention is produced by Xiwang Group and complies with GB / T 20884 standard; the vacuum freeze dryer described in the present invention is produced by Ningbo Xinzhi Biotechnology Co., Ltd. and has a model of scientz-100FB-S.

[0014] The spray dryer described in the present invention is produced by Junhe (Shanghai) Instrument Technology Co., Ltd. and its model is AH-PILOT.

[0015] The beneficial effect of the present invention is that the prepared pomegranate fruit fermentation product can inhibit DNA methylation, enhance CDK1 gene expression, and has an epigenetic mechanism of action.

[0016] To verify the beneficial effects of the present invention, the present invention conducted the following experiments.

[0017] DNA methylation test: In this experiment, human immortalized keratinocytes were used to establish an experimental model. The test method was to dilute the test sample and the control sample with water to 0.005% (mass percentage), respectively, and after acting on human immortalized keratinocytes, the relative expression level of the DNMT1 gene was detected to evaluate whether the sample had the effect of inhibiting DNA methylation.

[0018] Immortalized human keratinocytes were seeded into 6-well plates (6×10 5 holes), at 37 o C, 5% CO2 for 24 h. After the incubation, the culture medium was removed, and the cells were washed 1-2 times with D-Hanks. The normal control group was replaced with fresh culture medium, and the sample group was added with fresh culture medium containing the sample. The cells were then incubated at 37 o C, 5% CO2 for 24 h.

[0019] Total RNA was extracted from each experimental group, and cDNA was synthesized. The relative expression levels of the internal reference β-actin and the target gene DNMT1 were determined by q-PCR technology.

[0020] Table 1 Test samples and control samples Test sample 1 Pomegranate fermented product prepared in Example 1 of the present invention Test sample 2 Pomegranate fermented product prepared in Example 2 of the present invention Test sample 3 Pomegranate fermented product prepared in Example 3 of the present invention Test sample 4 Pomegranate fermented product prepared in Example 4 of the present invention Test sample 5 Pomegranate fermented product prepared in Example 5 of the present invention Control sample 1 Commercially available pomegranate fermentation Control sample 2 The other methods are as in Example 2 of the present invention, but only a single lactobacillus, i.e., a pomegranate fermented product fermented with Lactobacillus reuteri ATCC PTA6475, is used. Control sample 3 The other methods are as in Example 2 of the present invention, but only a single lactobacillus, i.e., a pomegranate fermented product fermented by Lactobacillus ATCC 53103 is used. Control sample 4 The other methods are as in Example 2 of the present invention, but only a single lactobacillus, i.e., pomegranate fermented by Lactobacillus plantarum ATCC 8014, is used. Control sample 5 The rest is as in Example 2 of the present invention, but Lactobacillus reuteri ATCC PTA6475, Lactobacillus acidobacillus ATCC 53103, and Lactobacillus plantarum ATCC 8014 are in a mass ratio of 0.5:1:5. Table 2 Relative expression of DNMT1 gene Test samples Relative expression of DNMT1 gene P-value Test results Test sample 1 0.781 ＜0.05 Significant Test sample 2 0.752 ＜0.05 Significant Test sample 3 0.739 ＜0.05 Significant Test sample 4 0.699 ＜0.05 Significant Test sample 5 0.716 ＜0.05 Significant Control sample 1 0.992 ＞0.05 Not significant Control sample 2 0.986 ＞0.05 Not significant Control sample 3 0.975 ＞0.05 Not significant Control sample 4 0.987 ＞0.05 Not significant Control sample 5 0.979 ＞0.05 Not significant As can be seen from Table 2, the pomegranate fermented product prepared in the embodiment of the present invention significantly reduces the relative expression of the DNMT1 gene compared with the normal control group, while the pomegranate fermented product not of the present invention or the pomegranate fermented product not using the preparation process of the present invention has no obvious effect on the relative expression of the DNMT1 gene, indicating that the pomegranate fermented product prepared in the present invention has the effect of reducing the relative expression of the DNMT1 gene, that is, it has the effect of inhibiting DNA methylation.

[0021] CDK1 gene expression test: In this experiment, human dermal fibroblasts were used to construct an experimental model, and the relative expression level of the CDK1 gene was detected to evaluate whether the sample had the effect of enhancing the expression of the CDK1 gene.

[0022] 1. Cells were seeded into 6-well plates (6×10 5 holes), at 37 o C, 5% CO2 incubation for 24 h.

[0023] 2. Remove the culture medium and gently rinse the cells once or twice with D-Hanks. Replace the culture medium with fresh medium for the normal control group and the culture medium containing the sample group at 37 °C. o C, 5% CO2 incubation for 24 h.

[0024] 3. Total RNA was extracted from each experimental group, cDNA was synthesized, and the relative expression levels of the internal reference β-actin and the target gene CDK1 were detected using q-PCR technology.

[0025] Table 3 CDK1 gene relative expression test values Test samples Relative expression of CDK1 gene P-value Test results Test sample 1 1.49 ＜0.01 Significant Test sample 2 1.53 ＜0.001 Significant Test sample 3 1.57 ＜0.001 Significant Test sample 4 1.61 ＜0.001 Significant Test sample 5 1.55 ＜0.001 Significant Control sample 1 1.06 ＞0.05 Not significant Control sample 2 1.08 ＞0.05 Not significant Control sample 3 1.11 ＞0.05 Not significant Control sample 4 1.09 ＞0.05 Not significant Control sample 5 1.06 ＞0.05 Not significant As can be seen from Table 3, the pomegranate fermented product prepared in the embodiment of the present invention significantly increases the relative expression of the CDK1 gene compared with the normal control group, while the pomegranate fermented product not of the present invention or the pomegranate fermented product not using the indicator process of the present invention has no obvious effect on the expression of the CDK1 gene, indicating that the pomegranate fermented product prepared in the present invention has the effect of increasing the expression of the CDK1 gene, and the sample has the effect of increasing the expression of the CDK1 gene.

[0026] It can be seen from Table 2 and Table 3 that the pomegranate fermented product of the present invention can inhibit DNA methylation, increase CDK1 gene expression, and has an epigenetic mechanism. DETAILED DESCRIPTION

[0027] Example 1: A pomegranate fermented product with an epigenetic mechanism of action, characterized in that it is prepared by the following method: A. Peel the fresh ripe pomegranate fruit, weigh 300 g of the pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 68°C for 40 min; B. Add 3g / L mixed lactobacillus, 20g / L casein and 25g / L fructose to pomegranate pulp, adjust the pH of the fermentation liquid to 6 with L-arginine, and culture at 36.5°C for 72h. During this period, use high performance liquid chromatography to test the content of urolithin A in the fermentation liquid, and the fermentation end point is when urolithin A reaches 12g / L; C. Filter using a 300-mesh filter cloth. The filtrate is the pomegranate fruit fermentation liquid. D. Using a vacuum freeze dryer, adjust the temperature to -80°C to vacuum freeze-dry the pomegranate fruit fermentation liquid containing urolithin A for 48 h to obtain a pomegranate fruit fermentation dry basis; E. Mix and dissolve the above fermented product dry basis and 85% ethanol by volume in a mass ratio of 1 to 20, filter and remove alcohol insoluble matter using a 300-mesh filter cloth, and use a vacuum freeze dryer again to adjust the temperature to -80°C to remove ethanol and water to obtain a refined pomegranate fruit fermented product dry basis; F. According to a mass ratio of 1:4, the above fermentation dry basis and maltodextrin are mechanically mixed to obtain a mixture, and then the mixture is spray-dried after being completely dissolved with deionized water in a mass ratio of 1:50. The inlet air temperature is set to 160°C, the outlet temperature is set to 60°C, the feed rate is set to 300ml / h, the spray working pressure is set to 0.3Mpa, the air volume is set to 2.5 cubic meters / minute, and the moisture content of the spray-dried powder is controlled to be no higher than 3%. The spray-dried powder is the pomegranate fermentation of the present invention.

[0028] Example 2: A pomegranate fermented product with an epigenetic mechanism of action, characterized in that it is prepared by the following method: A. Peel the fresh ripe pomegranate fruit, weigh 350 g of pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 68.5℃ for 37 min; B. Add 3.5 g / L mixed lactobacillus, 18 g / L casein and 30 g / L fructose to pomegranate pulp, adjust the pH of the fermentation liquid to 6.5 with L-arginine, and culture at 36.8°C for 52 h. During this period, use high performance liquid chromatography to test the content of urolithin A in the fermentation liquid, and the fermentation end point is when urolithin A reaches 12 g / L; C. Filter using a 300-mesh filter cloth. The filtrate is the pomegranate fruit fermentation liquid. D. Using a vacuum freeze dryer, adjust the temperature to -75°C to vacuum freeze-dry the pomegranate fruit fermentation liquid containing urolithin A for 48 h to obtain a dry basis of pomegranate fruit fermentation material; E. The above fermentation dry basis and 85% ethanol by volume concentration were mixed and dissolved in a mass ratio of 1:15, and a 300-mesh filter cloth was used to filter out the alcohol-insoluble matter, and the alcohol-soluble matter was again treated with a vacuum freeze dryer, the temperature was adjusted to -75°C, and the ethanol and water were removed to obtain a refined pomegranate fruit fermentation dry basis; F. According to a mass ratio of 1:6, the above fermentation dry basis and maltodextrin are mechanically mixed to obtain a mixture, and then the mixture and deionized water are mixed in a mass ratio of 1:50, and the mixture is spray-dried after being completely dissolved. The inlet air temperature is set to 150°C, the outlet temperature is set to 65°C, the feed rate is set to 320ml / h, the spray working pressure is set to 0.35Mpa, the air volume is set to 2.5 cubic meters / minute, and the moisture content of the spray-dried powder is controlled to be no higher than 3%. The spray-dried powder is the pomegranate fermentation of the present invention.

[0029] Example 3: A pomegranate fermented product with an epigenetic mechanism of action, characterized in that it is prepared by the following method: A. Peel the fresh ripe pomegranate fruit, weigh 400 g of pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 69°C for 35 min; B. Add 4 g / L mixed lactobacillus, 15 g / L casein and 35 g / L fructose to pomegranate pulp, adjust the pH of the fermentation liquid to 7 with L-arginine, and culture at 37°C for 56 h. During this period, use high performance liquid chromatography to test the urolithin A content in the fermentation liquid, and the fermentation end point is when urolithin A reaches 12 g / L; C. Filter using a 300-mesh filter cloth. The filtrate is the pomegranate fruit fermentation liquid. D. Using a vacuum freeze dryer, adjust the temperature to -70°C and perform vacuum freeze drying on the pomegranate fruit fermentation liquid containing urolithin A for 48 h to obtain a pomegranate fruit fermentation dry basis; E. The above fermentation dry basis and 85% ethanol by volume concentration were mixed and dissolved in a mass ratio of 1:10, and a 300-mesh filter cloth was used to filter out the alcohol-insoluble matter, and the alcohol-soluble matter was again treated with a vacuum freeze dryer, the temperature was adjusted to -70°C, and the ethanol and water were removed to obtain a refined pomegranate fruit fermentation dry basis; F. The above fermentation product dry basis and maltodextrin are mechanically mixed at a mass ratio of 1:7 to obtain a mixture, and then the mixture is spray-dried after being completely dissolved with deionized water at a mass ratio of 1:50. The inlet air temperature is set to 140°C, the outlet temperature is set to 70°C, the feed rate is set to 340ml / h, the spray working pressure is set to 0.4Mpa, the air volume is set to 2.5 cubic meters / minute, and the moisture content of the spray-dried powder is controlled to be no higher than 3%. The spray-dried powder is the pomegranate fermentation product of the present invention.

[0030] Example 4: A pomegranate fermented product with an epigenetic mechanism of action, characterized in that it is prepared by the following method: A. Peel the fresh ripe pomegranate fruit, weigh 450 g of pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 68.5℃ for 33 min; B. Add 5 g / L mixed lactobacillus, 12 g / L casein and 40 g / L fructose to pomegranate pulp, adjust the pH of the fermentation liquid to 7.5 with L-arginine, and culture at 37.2°C for 65 h. During this period, use high performance liquid chromatography to test the urolithin A content in the fermentation liquid, and the fermentation end point is when urolithin A reaches 12 g / L; C. Filter using a 300-mesh filter cloth. The filtrate is the pomegranate fruit fermentation liquid. D. Using a vacuum freeze dryer, adjust the temperature to -65°C to vacuum freeze-dry the pomegranate fruit fermentation liquid containing urolithin A for 48 h to obtain a pomegranate fruit fermentation dry basis; E. The above fermentation dry basis and 85% ethanol by volume concentration were mixed and dissolved in a mass ratio of 1:8, and the alcohol insoluble matter was filtered out using a 300-mesh filter cloth. The alcohol-soluble matter was again subjected to a vacuum freeze dryer, and the temperature was adjusted to -65°C to remove ethanol and water to obtain a refined pomegranate fruit fermentation dry basis; F. According to a mass ratio of 1:8, the above fermentation dry basis and maltodextrin are mechanically mixed to obtain a mixture, and then the mixture and deionized water are mixed in a mass ratio of 1:50, and the mixture is spray-dried after being completely dissolved. The inlet air temperature is set to 130°C, the outlet temperature is set to 80°C, the feed rate is set to 370ml / h, the spray working pressure is set to 0.45Mpa, the air volume is set to 2.5 cubic meters / minute, and the moisture content of the spray-dried powder is controlled to be no higher than 3%. The spray-dried powder is the pomegranate fermentation of the present invention.

[0031] Example 5: A pomegranate fermented product with an epigenetic mechanism of action, characterized in that it is prepared by the following method: A. Peel the fresh ripe pomegranate fruit, weigh 500 g of pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 70°C for 30 min; B. Add 6 g / L mixed lactobacillus, 10 g / L casein and 50 g / L fructose to pomegranate pulp, adjust the pH of the fermentation liquid to 8 with L-arginine, and culture at 37.7°C for 72 h. During this period, use high performance liquid chromatography to test the content of urolithin A in the fermentation liquid, and the fermentation end point is when urolithin A reaches 12 g / L; C. Filter using a 300-mesh filter cloth. The filtrate is the pomegranate fruit fermentation liquid. D. Using a vacuum freeze dryer, adjust the temperature to -60°C to vacuum freeze-dry the pomegranate fruit fermentation liquid containing urolithin A for 48 h to obtain a pomegranate fruit fermentation dry basis; E. The above fermentation dry basis and 85% ethanol by volume concentration were mixed and dissolved in a mass ratio of 1:5, and a 300-mesh filter cloth was used to filter out the alcohol-insoluble matter, and the alcohol-soluble matter was again treated with a vacuum freeze dryer, the temperature was adjusted to -60°C, and the ethanol and water were removed to obtain a refined pomegranate fruit fermentation dry basis; F. According to a mass ratio of 1:10, the above fermentation dry basis and maltodextrin are mechanically mixed to obtain a mixture, and then the mixture and deionized water are spray-dried after being completely dissolved in a mass ratio of 1:50. The inlet air temperature is set to 120°C, the outlet temperature is set to 90°C, the feed rate is set to 300ml / h, the spray working pressure is set to 0.5Mpa, the air volume is set to 2.5 cubic meters / minute, and the moisture content of the spray-dried powder is controlled to be not higher than 3%. The spray-dried powder is the pomegranate fermentation of the present invention.

Claims

1. A pomegranate fermented product with an epigenetic mechanism of action, characterized in that Prepared by the following method: A. Peel the fresh ripe pomegranate fruit, weigh 300-500 g of pulp, use a wall-breaking machine to break the pulp and homogenize it to obtain pomegranate pulp, and pasteurize it at 68-70℃ for 30-40 min; B. Add 3-6 g / L mixed lactobacillus, 10-20 g / L casein and 25-50 g / L fructose to the pomegranate pulp described in step A, adjust the pH of the fermentation broth to 6-8 using L-arginine, and culture for 48-72 h at 36.5-37.7 ° C. During the culture, the urolithin A content in the fermentation broth was tested by high performance liquid chromatography, and the fermentation end point was 12 g / L of urolithin A; the mixed lactobacillus was mixed by Lactobacillus reuteri ATCC PTA6475, Lactobacillus ATCC 53103 and Lactobacillus plantarum ATCC 8014 in a mass ratio of 1:1:1; C. Filter using a 300-mesh filter cloth to obtain pomegranate fruit fermentation liquid; D. Using a vacuum freeze dryer, adjusting the temperature to -80~-60°C, vacuum freeze-drying the pomegranate fruit fermentation liquid for 48 h to obtain a fermentation dry basis; E. The fermented product dry basis and 85% ethanol by volume concentration are mixed and dissolved in a mass ratio of 1:5-20, and a 300-mesh filter cloth is used to filter and remove the alcohol-insoluble matter. The alcohol-soluble matter is freeze-dried again using a vacuum freeze dryer at a freeze-drying temperature of -80 to -60 ° C, and the ethanol and water are removed to obtain a refined pomegranate fruit fermented product dry basis; F. Weigh the pomegranate fruit fermentation dry basis and maltodextrin obtained in step E at a mass ratio of 1: 4-10, mechanically mix them uniformly to obtain a premix, mix and dissolve the premix with deionized water at a mass ratio of 1:50, spray dry after complete dissolution, and spray dry under the conditions of: inlet air temperature of 120-160° C., outlet temperature of 60-90° C., feed rate of 300-400 ml / h, spray working pressure of 0.3-0.5 MPa, air volume of 2.5 cubic meters / minute, and control the moisture content of the spray-dried powder to be no higher than 3%. The obtained spray-dried powder is the pomegranate fermentation with an epigenetic mechanism of action.

2. Use of the pomegranate fermented product with epigenetic mechanism of action as claimed in claim 1 in cosmetics.