A method for evaluating the soothing effect of cosmetics

Abstract

The present invention relates to the technical field of data processing, and in particular to a method for evaluating the soothing effect of cosmetics; the method comprises the following steps: S1, modeling, wherein a sample area and a control area are arranged on the arm of each subject; S2, obtaining measurement data of the sample area and the control area on the arm of each subject, and obtaining change data of the control area and the sample area of ​​each subject; S3, performing paired statistical analysis on the change data between the control area and the sample area of ​​all subjects, and obtaining a redness value p value, a redness region area p value, a wheal area p value, and a wheal volume p value; S4, obtaining a total test score of a test product; S5, setting a first score value and a second score value, and evaluating the test product, wherein the evaluation grades include no soothing effect, general soothing effect, and good soothing effect; the present invention realizes the objectivity of the evaluation result and improves the accuracy of the evaluation result.

Description

Technical Field

[0001] The present invention relates to the technical field of data processing, and in particular to a method for evaluating the soothing effect of cosmetics. Background Art

[0002] Cosmetic efficacy claim tests are generally divided into human efficacy evaluation tests, consumer use tests, in vitro efficacy evaluation tests, etc. Among them, the human efficacy evaluation test is controlled by factors such as the usage scenario and the test environment, and the test is conducted by reasonably setting up a blank control group or a negative control group, so the credibility of the test results is relatively high.

[0003] In most human trials of the soothing effect of cosmetics, subjects with irritated skin, such as redness, dryness and itchiness, are recruited. The subjects are asked to use the product according to the requirements, and the soothing effect of the product is evaluated by measuring the changes in skin condition before and after using the product. The skin condition is usually expressed by the skin redness value. In some human trials of the soothing effect of cosmetics, methyl nicotinate, capsaicin, lactic acid, histamine and other reagents are first used to cause redness, itching, stinging, burning and other irritation conditions on the skin. After the model is completed, the product is used according to the requirements, and the soothing effect of the product is evaluated by measuring the changes in skin condition before and after using the product and obtaining the subject's experience.

[0004] Methyl nicotinate is used for modeling because it can cause vasodilation and thus produce redness of the skin. However, the mechanism of action of methyl nicotinic acid in causing vasodilation is still unclear. Furthermore, capsaicin and lactic acid are used for modeling, so the soothing effect of the product can only be evaluated through semi-subjective pain analysis. At the same time, due to individual differences, each subject has different stimulation sensations to the same concentration of capsaicin or lactic acid, so the modeling using capsaicin and lactic acid leads to inaccurate evaluation results. Therefore, most of the current models use histamine, which is applied to the subject's skin to cause redness and itching at the applied site. Then the test product is applied to the subject's skin, and the soothing effect of the test product is evaluated by the redness value and itching degree before and after the subject uses the product.

[0005] However, the evaluation factors in the existing evaluation methods are all subjective to a certain extent, and different subjects have different subjective feelings, which will lead to the evaluation results of the test products being not objective and accurate enough, and the evaluation results cannot be quantified.

[0006] Therefore, there is an urgent need to provide a method for evaluating the soothing effect of cosmetics, which can achieve the objectivity of the evaluation results and improve the accuracy of the evaluation results compared with the existing technology. Summary of the invention

[0007] The present invention solves the technical problems existing in the prior art and provides a method for evaluating the soothing effect of cosmetics.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0009] A method for evaluating the soothing effect of cosmetics, comprising the following steps:

[0010] S1. Use histamine hydrochloride aqueous solution to model the arms of multiple subjects, and each subject's arm is provided with a sample area and a control area;

[0011] S2, applying the test product only to the sample area on the arm of each subject, obtaining measurement data of the sample area and the control area on the arm of each subject before applying the product and after a set time, the measurement data including redness value, redness area, wheal area and wheal volume, comparing the measurement data obtained in the control area and sample area of ​​each subject with the measurement data before using the product one by one, obtaining change data of the control area and sample area of ​​each subject, the change data including change value of redness value, change value of redness area, change value of wheal area and change value of wheal volume;

[0012] S3, performing paired statistical analysis on the redness value change values, redness area change values, wheal area change values ​​and wheal volume change values ​​between the control area and the sample area of ​​all subjects to obtain the redness value p value, redness area p value, wheal area p value and wheal volume p value;

[0013] S4. Obtain the total test score of the test product according to the redness value p value, the redness area p value, the wheal area p value, and the wheal volume p value;

[0014] S5. Set a first scoring value and a second scoring value, the first scoring value < the second scoring value; when the total test score < the first scoring value, the test product is evaluated as having no soothing effect; when the first scoring value ≤ the total test score < the second scoring value, the test product is evaluated as having a general soothing effect; when the total test score ≥ the second scoring value, the test product is evaluated as having a good soothing effect.

[0015] Furthermore, multiple set times are set in step S2, and all set times increase in sequence, and after each set time, the change data of all subjects are obtained;

[0016] Multiple set times constitute a set time set, which is expressed as:

[0017] T={T 1 ,…,T j ,…,T M};

[0018] T M≤30min;

[0019] In the above formula, T represents the set time set, T 1 Indicates the first setting time, T j represents the jth setting time, T M Indicates the Mth setting time, j ranges from 1 to M.

[0020] Furthermore, the total test score of the product tested in step S4 is calculated according to the following formula:

[0021]

[0022] In the above formula, S represents the total test score of the tested product, ΔS j represents the test score corresponding to the jth setting time of the test product, ω j Indicates ΔS j The corresponding weight value.

[0023] Furthermore, ΔS j Calculated by the following formula:

[0024]

[0025] In the above formula, It represents the test score corresponding to the redness value p obtained after j set time periods, It represents the test score corresponding to the red area p value obtained after j set time periods, It represents the test score corresponding to the wind mass area p value obtained after j set time periods. It represents the test score corresponding to the wheal volume p value obtained after j set time periods.

[0026] Furthermore, the correspondence between the test score and the p-value is expressed as:

[0027] (1) When the measured data of the test area on the subject's arm is worse than the measured data of the control area: when the p value is less than 0.01, the corresponding test score is -3 points; when the p value is greater than or equal to 0.01 and less than or equal to 0.05, the corresponding test score is -1 point; when the p value is greater than 0.05, the corresponding test score is 0 points;

[0028] (2) When the measured data of the test area on the subject's arm is better than the measured data of the control area, when the p value is less than 0.01, the corresponding test score is 3 points; when the p value is greater than or equal to 0.01 and less than or equal to 0.05, the corresponding test score is 1 point; when the p value is greater than 0.05, the corresponding test score is 0 points.

[0029] Furthermore, the weight values ​​corresponding to all set times are set according to the following formula:

[0030] ω 1 +ω 2 +…+ω j +…+ω M =1;

[0031] The weight value corresponding to the 1st setting time to the weight value corresponding to the M / 2th setting time is set by the following formula:

[0032]

[0033] The weight value corresponding to the (M / 2+1)th setting time to the weight value corresponding to the Mth setting time is set by the following formula:

[0034]

[0035] In the above formula, ω j represents the weight value corresponding to the jth setting time, ω (j-1) represents the weight value corresponding to the (j-1)th setting time, T j Indicates the specific time value corresponding to the jth set time, T (j-1) Indicates the specific time value corresponding to the (j-1)th set time.

[0036] Furthermore, in step S2, the redness value change value, redness area change value, wheal area change value and wheal volume change value of the control area and sample area of ​​each subject after each set time are calculated by the following formula:

[0037]

[0038] In the above formula, It represents the change in redness value of the control area of ​​the i-th subject after the j-th set time. represents the redness value of the control area of ​​the i-th subject before using the product, It represents the redness value of the control area of ​​the i-th subject after the j-th set time; It represents the change in redness value of the sample area of ​​the i-th subject after the j-th set time. represents the redness value of the sample area before the i-th subject uses the product, It represents the redness value of the sample area after the jth set time of the i-th subject; It represents the change in the red area of ​​the control area of ​​the i-th subject after the j-th set time. represents the reddened area of ​​the control area of ​​the i-th subject before using the product, It represents the reddened area of ​​the control area of ​​the i-th subject after the j-th set time; It represents the change in the red area of ​​the sample area of ​​the i-th subject after the j-th set time. represents the reddened area of ​​the sample area before the i-th subject uses the product, It represents the reddish area of ​​the sample area after the jth set time of the i-th subject; It represents the change in the wheal area of ​​the control area of ​​the ith subject after the jth set time. represents the wheal area of ​​the control area of ​​the i-th subject before using the product, It represents the wheal area of ​​the control area of ​​the i-th subject after the j-th set time; It represents the change in the wheal area of ​​the sample area of ​​the i-th subject after the j-th set time. represents the wheal area of ​​the sample area before the i-th subject uses the product, It represents the wheal area of ​​the sample area after the jth set time of the i-th subject; It represents the change in the wheal volume of the control area of ​​the ith subject after the jth set time. represents the wheal volume of the control area of ​​the i-th subject before using the product, It represents the wheal volume of the control area of ​​the i-th subject after the j-th set time; It represents the change in the wheal volume of the sample area of ​​the ith subject after the jth set time. represents the wheal volume of the sample area before the i-th subject uses the product, It represents the wheal volume of the sample area after the jth set time for the i-th subject.

[0039] Furthermore, the redness value p value, the redness area p value, the wheal area p value and the wheal volume p value after each set time are specifically obtained by the following method:

[0040] The redness value change values, redness region area change values, wheal area change values, and wheal volume change values ​​of the control areas of all subjects obtained at the j-th set time are respectively set as the redness value control data group, the redness region area control data group, the wheal area control data group, and the wheal volume control data group; the redness value change values, redness region area change values, wheal area change values, and wheal volume change values ​​of the sample areas of all subjects are respectively set as the redness value sample data group, the redness region area sample data group, the wheal area sample data group, and the wheal volume sample data group; the redness value control data group corresponds to the redness value sample data group, the redness region area control data group corresponds to the redness region area sample data group, the wheal area control data group corresponds to the wheal area sample data group, and the wheal volume control data group corresponds to the wheal volume sample data group;

[0041] The corresponding control data group and sample data group were paired for statistical analysis. If the paired difference between the corresponding control data group and the sample data group was normally distributed, a paired t-test was used. If it was not normally distributed, a rank sum test was used. A two-tailed test was performed to obtain the redness value p value, redness area p value, wheal area p value and wheal volume p value after the j-th set time.

[0042] Furthermore, S1 specifically includes the following steps:

[0043] S101, cleaning the arm of each subject, marking two areas of equal area on the arm of each subject, which are set as a first area and a second area;

[0044] S102, performing microneedle pressing on the first area and the second area on the arm of each subject;

[0045] S103, applying filter paper sheets soaked in histamine hydrochloride aqueous solution of the same concentration to the first area and the second area of ​​each subject for a predetermined time;

[0046] S104, after a predetermined time, remove all filter paper sheets, and use absorbent paper to absorb the liquid in the first area and the second area on the subject's arm to complete the modeling;

[0047] S105, setting the first area processed in step S104 as the sample area, and the second area as the control area.

[0048] Furthermore, in step S102, a pressing device is used to press the microneedles, and the pressing device includes an outer nut, an outer shell, a transmission rod, a microneedle matrix and a baffle, the outer shell is sleeved on one end of the outer shell, the baffle covers the end of the outer shell away from the outer nut, the transmission rod and the microneedle matrix are arranged inside the outer shell, one end of the transmission rod is connected to the outer nut, the other end of the transmission rod is fixedly connected to the microneedle matrix, the outer nut is rotatably connected to the outer shell; the transmission rod is threadedly connected to the outer nut, and a digital label is provided on the outside of the outer nut.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] The present invention collects measurement data such as redness values, redness area and volume, wheal area and wheal volume of multiple subjects, sets multiple set times, collects measurement data after each set time, obtains change data of each measurement data at each set time, processes the change data, and obtains a p-value corresponding to each measurement data at each set time, constructs a relationship between the p-value and the test score, and sets different weight values ​​for the test scores corresponding to different set times. By considering multiple factors, a more accurate total measurement score is obtained, thereby making the evaluation of the test product more accurate and objective. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 is a flow chart of the method of the present invention.

[0052] Figure 2 It is a schematic structural diagram of the pressing device of the present invention.

[0053] Figure 3 It is a schematic diagram of the outer nut in the pressing device of the present invention.

[0054] Description of reference numerals:

[0055] 1. External nut; 2. External shell; 3. Transmission rod; 4. Microneedle matrix; 5. Baffle; 6. Digital label. DETAILED DESCRIPTION

[0056] The technical solution of the present invention will be clearly described below in conjunction with the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without creative work belong to the protection scope of the present invention. It should be noted that the orientation or position relationship indicated by the terms "center", "up", "down", "left", "right", "vertical", "horizontal", etc. is based on the orientation or position relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description. It does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0057] like Figure 1 As shown, the present invention provides a method for evaluating the soothing effect of cosmetics, comprising the following steps:

[0058] S1. Modeling is performed on the arms of multiple subjects using histamine hydrochloride aqueous solution, with each subject's arm having a sample area and a control area. Specifically, the following steps are included:

[0059] S101. Clean the arm of each subject with soapy water and wait for at least 20 minutes after cleaning. During the waiting period, mark two areas of equal area on the arm of each subject, set as the first area and the second area. The area of ​​the first area and the second area is 3 cm×3 cm, and the interval between the first area and the second area is at least 2 cm.

[0060] S102, performing microneedle pressing on the first area and the second area on the arm of each subject, specifically using a pressing device to perform microneedle pressing.

[0061] like Figure 2 , Figure 3 As shown, the pressing device includes an outer nut 1, an outer shell 2, a transmission rod 3, a microneedle matrix 4 and a baffle 5. The outer shell 2 is sleeved on one end of the outer shell 2, and the baffle 5 covers the end of the outer shell 2 away from the outer nut 1. The baffle 5 is provided with a plurality of pinholes, and the microneedle matrix 4 is provided with a plurality of microneedles. The pinholes are used to facilitate the microneedles to extend out of the outer shell 2. The transmission rod 3 and the microneedle matrix 4 are arranged inside the outer shell 2. One end of the transmission rod 3 is connected to the outer nut 1, and the other end of the transmission rod 3 is fixedly connected to the microneedle matrix 4. The outer nut 1 is rotatably connected to the outer shell 2. The transmission rod 3 is threadedly connected to the outer nut 1, and a digital label 6 is arranged on the outside of the outer nut 1. The baffle 5 is attached to the skin of the subject's arm, and the outer nut 1 is rotated, and the transmission rod 3 moves downward, thereby driving the microneedle matrix 4 to move in the direction close to the subject, and the microneedles extend out of the outer shell 2 through the pinholes to press the subject's arm.

[0062] S103, prepare a 0.5% histamine hydrochloride aqueous solution, immerse multiple filter papers in the prepared histamine hydrochloride aqueous solution, select qualitative filter papers with a diameter of 1 cm, and stick the filter papers immersed in the histamine hydrochloride aqueous solution of the same concentration on the first area and the second area of ​​each subject for a predetermined time, preferably 2 minutes.

[0063] S104. After a predetermined time, all filter paper sheets are removed, and the liquid in the first area and the second area on the subject's arm is absorbed with absorbent paper to complete the modeling.

[0064] S105, setting the first area processed in step S104 as the sample area, and the second area as the control area.

[0065] S2. Apply the test product only to the sample area on the arm of each subject. Before applying the product and after a set time, obtain measurement data of the sample area and control area on the arm of each subject, the measurement data including redness value, redness area, wheal area and wheal volume. Compare the measurement data obtained in the control area and sample area of ​​each subject with the measurement data before applying the product one by one to obtain change data of the control area and sample area of ​​each subject, the change data including change value of redness value, change value of redness area, change value of wheal area and change value of wheal volume.

[0066] The measurement data of the sample area and the control area on the subject's arm were obtained by measuring the 3D skin analysis system model "Antera 3D" of Miravex Company of Ireland.

[0067] In step S2, there are multiple set times, all of which are set in increasing order, and the longest set time is less than or equal to 30 minutes. After each set time, measurement data of the sample area and the control area on the arm are obtained once for each subject, so as to obtain the change data of each subject after each set time.

[0068] Multiple set times constitute a set time set, which is expressed as:

[0069] T={T 1 ,…,T j ,…,T M};

[0070] T M ≤30min;

[0071] In the above formula, T represents the set time set, T 1 Indicates the first setting time, T j represents the jth setting time, T M Indicates the Mth setting time, j ranges from 1 to M.

[0072] The change data of the control area and sample area of ​​each subject, namely, the change value of the redness value of the control area, the change value of the redness value of the sample area, the change value of the redness area of ​​the control area, the change value of the redness area of ​​the sample area, the change value of the wheal area of ​​the control area, the change value of the wheal area of ​​the sample area, the change value of the wheal volume of the control area, the change value of the wheal volume of the sample area; are specifically calculated by the following formula:

[0073]

[0074]

[0075] In the above formula, It represents the change in redness value of the control area of ​​the i-th subject after the j-th set time. represents the redness value of the control area of ​​the i-th subject before using the product, It represents the redness value of the control area of ​​the i-th subject after the j-th set time; It represents the change in redness value of the sample area of ​​the i-th subject after the j-th set time. represents the redness value of the sample area before the i-th subject uses the product, It represents the redness value of the sample area after the jth set time for the i-th subject. It represents the change in the red area of ​​the control area of ​​the i-th subject after the j-th set time. represents the reddened area of ​​the control area of ​​the i-th subject before using the product, It represents the reddened area of ​​the control area of ​​the i-th subject after the j-th set time; It represents the change in the red area of ​​the sample area of ​​the i-th subject after the j-th set time. represents the reddened area of ​​the sample area before the i-th subject uses the product, It represents the reddish area of ​​the sample area after the jth set time for the i-th subject. It represents the change in the wheal area of ​​the control area of ​​the ith subject after the jth set time. represents the wheal area of ​​the control area of ​​the i-th subject before using the product, It represents the wheal area of ​​the control area of ​​the i-th subject after the j-th set time; It represents the change in the wheal area of ​​the sample area of ​​the i-th subject after the j-th set time. represents the wheal area of ​​the sample area before the i-th subject uses the product, It represents the wheal area of ​​the sample area after the jth set time for the ith subject. It represents the change in the wheal volume of the control area of ​​the ith subject after the jth set time. represents the wheal volume of the control area of ​​the i-th subject before using the product, It represents the wheal volume of the control area of ​​the i-th subject after the j-th set time; It represents the change in the wheal volume of the sample area of ​​the ith subject after the jth set time. represents the wheal volume of the sample area before the i-th subject uses the product, It represents the wheal volume of the sample area after the jth set time for the i-th subject.

[0076] S3. Perform paired statistical analysis on the redness value change values, redness area change values, wheal area change values ​​and wheal volume change values ​​between the control area and the sample area of ​​all subjects to obtain the redness value p value, redness area p value, wheal area p value and wheal volume p value corresponding to each set time.

[0077] The redness value p after the jth set time is obtained by the following method:

[0078] The redness value change values ​​of the control areas of all subjects obtained at the j-th set time are set as the redness value control data group, and the redness value change values ​​of the sample areas of all subjects are set as the redness value sample data group. The two data groups are paired for statistical analysis. If the paired difference of the two data groups is normally distributed, a paired t-test is used. If it is not normally distributed, a rank sum test of two related samples is used for a two-tailed test to obtain the p value of the redness value after the j-th set time.

[0079] The reddening area p value after the jth set time is obtained by the following method:

[0080] The reddened area of ​​the control zone of all subjects obtained at the j-th set time is set as the reddened area control data group, and the reddened area of ​​the sample zone of all subjects is set as the reddened area sample data group. The two data groups are paired for statistical analysis. If the difference between the two paired data groups is normally distributed, a paired t-test is used. If it is not normally distributed, a rank sum test of two related samples is used for a two-tailed test to obtain the p-value of the reddened area after the j-th set time.

[0081] The wind mass area p value after the jth set time is obtained by the following method:

[0082] The wheal area change values ​​of the control areas of all subjects obtained at the j-th set time are set as the wheal area control data group, and the wheal area change values ​​of the sample areas of all subjects are set as the wheal area sample data group. The two data groups are paired for statistical analysis. If the paired difference of the two data groups is normally distributed, a paired t-test is used. If it is not normally distributed, a rank sum test of two related samples is used for a two-tailed test to obtain the p-value of the wheal area after the j-th set time.

[0083] The wind mass volume p value after the jth set time is obtained by the following method:

[0084] The wheal volume change values ​​of the control areas of all subjects obtained at the j-th set time are set as the wheal volume control data group, and the wheal volume change values ​​of the sample areas of all subjects are set as the wheal volume sample data group. The two data groups are paired for statistical analysis. If the paired difference of the two data groups is normally distributed, a paired t-test is used. If it is not normally distributed, a rank sum test of two related samples is used for a two-tailed test to obtain the p-value of the wheal volume after the j-th set time.

[0085] S4. Obtain a total test score for the test product according to the redness value p value, the redness area p value, the wheal area p value, and the wheal volume p value after all the set times.

[0086] The total test score of the tested product is calculated according to the following formula:

[0087]

[0088]

[0089] In the above formula, S represents the total test score of the tested product, ΔS j represents the test score corresponding to the jth setting time of the test product, ω j Indicates ΔS j The corresponding weight value, It represents the test score corresponding to the redness value p obtained after j set time periods, It represents the test score corresponding to the red area p value obtained after j set time periods, It represents the test score corresponding to the wind mass area p value obtained after j set time periods. It represents the test score corresponding to the wheal volume p value obtained after j set time periods.

[0090] The correspondence between the test score and the p-value is expressed as:

[0091] (1) When the measured data of the test area on the subject's arm is worse than the measured data of the control area: when the p value is less than 0.01, the corresponding test score is -3 points; when the p value is greater than or equal to 0.01 and less than or equal to 0.05, the corresponding test score is -1 point; when the p value is greater than 0.05, the corresponding test score is 0 points;

[0092] (2) When the measured data of the test area on the subject's arm is better than the measured data of the control area, when the p value is less than 0.01, the corresponding test score is 3 points; when the p value is greater than or equal to 0.01 and less than or equal to 0.05, the corresponding test score is 1 point; when the p value is greater than 0.05, the corresponding test score is 0 points.

[0093] The weight values ​​corresponding to all set times are set according to the following formula:

[0094] ω 1 +ω 2 +…+ω j +…+ω M =1;

[0095] The weight value corresponding to the 1st setting time to the weight value corresponding to the M / 2th setting time is set by the following formula:

[0096]

[0097] The weight value corresponding to the (M / 2+1)th setting time to the weight value corresponding to the Mth setting time is set by the following formula:

[0098]

[0099] In the above formula, ω j represents the weight value corresponding to the jth setting time, ω (j-1) represents the weight value corresponding to the (j-1)th setting time, T j Indicates the specific time value corresponding to the jth set time, T (j-1) Indicates the specific time value corresponding to the (j-1)th set time.

[0100] S5. Set a first scoring value and a second scoring value, the first scoring value < the second scoring value; when the total test score < the first scoring value, the test product is evaluated as having no soothing effect; when the first scoring value ≤ the total test score < the second scoring value, the test product is evaluated as having a general soothing effect; when the total test score ≥ the second scoring value, the test product is evaluated as having a good soothing effect.

[0101] The present invention collects measurement data such as redness values, redness area and volume, wheal area and wheal volume of multiple subjects, sets multiple set times, collects measurement data after each set time, obtains change data of each measurement data at each set time, processes the change data, and obtains a p-value corresponding to each measurement data at each set time, constructs a relationship between the p-value and the test score, and sets different weight values ​​for the test scores corresponding to different set times. By considering multiple factors, a more accurate total measurement score is obtained, thereby making the evaluation of the test product more accurate and objective.

[0102] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for evaluating the soothing effect of cosmetics, characterized in that: The following steps are involved: S1. Use histamine hydrochloride aqueous solution to model the arms of multiple subjects, and each subject's arm is provided with a sample area and a control area; S2, applying the test product only to the sample area on the arm of each subject, obtaining measurement data of the sample area and the control area on the arm of each subject before applying the product and after a set time, the measurement data including redness value, redness area, wheal area and wheal volume, comparing the measurement data obtained in the control area and sample area of ​​each subject with the measurement data before using the product one by one, obtaining change data of the control area and sample area of ​​each subject, the change data including change value of redness value, change value of redness area, change value of wheal area and change value of wheal volume; S3, performing paired statistical analysis on the redness value change values, redness area change values, wheal area change values ​​and wheal volume change values ​​between the control area and the sample area of ​​all subjects to obtain the redness value p value, redness area p value, wheal area p value and wheal volume p value; S4. Obtain the total test score of the test product according to the redness value p value, the redness area p value, the wheal area p value, and the wheal volume p value; The total test score is calculated as follows: In the above formula, S represents the total test score of the tested product, ΔS j represents the test score corresponding to the jth setting time of the test product, ω j Indicates ΔS j The corresponding weight value, j, ranges from 1 to M; ΔS j Calculated by the following formula: In the above formula, It represents the test score corresponding to the redness value p obtained after j set time periods, It represents the test score corresponding to the red area p value obtained after j set time periods, It represents the test score corresponding to the wind mass area p value obtained after j set time periods. It represents the test score corresponding to the wheal volume p value obtained after j set time periods; The weight values ​​corresponding to all set times are set according to the following formula: ω1+ω2+…+ω j +…+oh M =1; The weight value corresponding to the 1st setting time to the weight value corresponding to the M / 2th setting time is set by the following formula: The weight value corresponding to the (M / 2+1)th setting time to the weight value corresponding to the Mth setting time is set by the following formula: In the above formula, ω j represents the weight value corresponding to the jth setting time, ω (j-1) represents the weight value corresponding to the (j-1)th setting time, T j Indicates the specific time value corresponding to the jth set time, T (j-1) Indicates the specific time value corresponding to the (j-1)th set time; S5. Set a first scoring value and a second scoring value, the first scoring value < the second scoring value; when the total test score < the first scoring value, the test product is evaluated as having no soothing effect; when the first scoring value ≤ the total test score < the second scoring value, the test product is evaluated as having a general soothing effect; when the total test score ≥ the second scoring value, the test product is evaluated as having a good soothing effect.

2. A method for evaluating the soothing effect of cosmetics according to claim 1, characterized in that: In step S2, multiple set times are set, and all set times increase in sequence. After each set time, the change data of all subjects are obtained; Multiple set times constitute a set time set, which is expressed as: T={T1,…,T j ,…,T M }; T M ≤30min; In the above formula, T represents the set time set, T1 represents the first set time, T j represents the jth setting time, T M Indicates the Mth setting time.

3. A method for evaluating the soothing effect of cosmetics according to claim 2, characterized in that: The correspondence between the test score and the p-value is expressed as: (1) When the measured data of the test area on the subject's arm is worse than the measured data of the control area: when the p value is less than 0.01, the corresponding test score is -3 points; when the p value is greater than or equal to 0.01 and less than or equal to 0.05, the corresponding test score is -1 point; when the p value is greater than 0.05, the corresponding test score is 0 points; (2) When the measured data of the test area on the subject's arm is better than the measured data of the control area, when the p value is less than 0.01, the corresponding test score is 3 points; when the p value is greater than or equal to 0.01 and less than or equal to 0.05, the corresponding test score is 1 point; when the p value is greater than 0.05, the corresponding test score is 0 points.

4. A method for evaluating the soothing effect of cosmetics according to claim 2, characterized in that: In step S2, the redness value change value of the control area and sample area, the redness area change value, the wheal area change value and the wheal volume change value of each subject after each set time are calculated by the following formula: In the above formula, It represents the change in redness value of the control area of ​​the i-th subject after the j-th set time. represents the redness value of the control area of ​​the i-th subject before using the product, It represents the redness value of the control area of ​​the i-th subject after the j-th set time; It represents the change in redness value of the sample area of ​​the i-th subject after the j-th set time. represents the redness value of the sample area before the i-th subject uses the product, It represents the redness value of the sample area after the jth set time of the i-th subject; It represents the change in the red area of ​​the control area of ​​the i-th subject after the j-th set time. represents the reddened area of ​​the control area of ​​the i-th subject before using the product, It represents the reddened area of ​​the control area of ​​the i-th subject after the j-th set time; It represents the change in the red area of ​​the sample area of ​​the i-th subject after the j-th set time. represents the reddened area of ​​the sample area before the i-th subject uses the product, It represents the reddish area of ​​the sample area after the jth set time of the i-th subject; It represents the change in the wheal area of ​​the control area of ​​the ith subject after the jth set time. represents the wheal area of ​​the control area of ​​the i-th subject before using the product, It represents the wheal area of ​​the control area of ​​the i-th subject after the j-th set time; It represents the change in the wheal area of ​​the sample area of ​​the i-th subject after the j-th set time. represents the wheal area of ​​the sample area before the i-th subject uses the product, It represents the wheal area of ​​the sample area after the jth set time of the i-th subject; It represents the change in the wheal volume of the control area of ​​the ith subject after the jth set time. represents the wheal volume of the control area of ​​the i-th subject before using the product, It represents the wheal volume of the control area of ​​the i-th subject after the j-th set time; It represents the change in the wheal volume of the sample area of ​​the i-th subject after the j-th set time. represents the wheal volume of the sample area before the i-th subject uses the product, It represents the wheal volume of the sample area after the jth set time for the i-th subject.

5. A method for evaluating the soothing effect of cosmetics according to claim 4, characterized in that: The redness value p value, redness area p value, wheal area p value and wheal volume p value after each set time are obtained by the following method: The redness value change values, redness region area change values, wheal area change values, and wheal volume change values ​​of the control areas of all subjects obtained at the j-th set time are respectively set as the redness value control data group, the redness region area control data group, the wheal area control data group, and the wheal volume control data group; the redness value change values, redness region area change values, wheal area change values, and wheal volume change values ​​of the sample areas of all subjects are respectively set as the redness value sample data group, the redness region area sample data group, the wheal area sample data group, and the wheal volume sample data group; the redness value control data group corresponds to the redness value sample data group, the redness region area control data group corresponds to the redness region area sample data group, the wheal area control data group corresponds to the wheal area sample data group, and the wheal volume control data group corresponds to the wheal volume sample data group; The corresponding control data group and sample data group were paired for statistical analysis. If the paired difference between the corresponding control data group and the sample data group was normally distributed, a paired t-test was used. If it was not normally distributed, a rank sum test was used. A two-tailed test was performed to obtain the redness value p value, redness area p value, wheal area p value and wheal volume p value after the j-th set time.

6. A method for evaluating the soothing effect of cosmetics according to claim 1, characterized in that: S1 specifically includes the following steps: S101, cleaning the arm of each subject, marking two areas of equal area on the arm of each subject, which are set as a first area and a second area; S102, performing microneedle pressing on the first area and the second area on the arm of each subject; S103, applying filter paper sheets soaked in histamine hydrochloride aqueous solution of the same concentration to the first area and the second area of ​​each subject for a predetermined time; S104, after a predetermined time, removing all filter paper sheets, and absorbing the liquid in the first area and the second area on the subject's arm with absorbent paper to complete the modeling; S105, setting the first area processed in step S104 as the sample area, and the second area as the control area.

7. A method for evaluating the soothing effect of cosmetics according to claim 6, characterized in that: In step S102, a pressing device is used to press the microneedles. The pressing device includes an outer nut, an outer shell, a transmission rod, a microneedle matrix and a baffle. The outer shell is sleeved on one end of the outer shell, and the baffle covers the end of the outer shell away from the outer nut. The transmission rod and the microneedle matrix are arranged inside the outer shell, one end of the transmission rod is connected to the outer nut, and the other end of the transmission rod is fixedly connected to the microneedle matrix. The outer nut is rotatably connected to the outer shell; the transmission rod is threadedly connected to the outer nut, and a digital label is provided on the outside of the outer nut.

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