Small and dense low-density lipoprotein cholesterol detection kit

Abstract

The invention relates to the technical field of detection kits, in particular to a small and dense low-density lipoprotein cholesterol detection kit which comprises a kit body, an inner protective plate is fixedly mounted at the inner end of the kit body, a temperature changer is fixedly mounted at the bottom end of the inner protective plate, a test tube is arranged on the inner side of the temperature changer, and a sealing screw cap is mounted at the upper end of the inner protective plate. A guide pipe is arranged at the upper end of the inner protective plate in a penetrating manner, an outer guide pipe is arranged on the inner side of the guide pipe, a fixing frame is detachably mounted at the upper end of the inner side of the kit, and a plurality of counting devices for adding different reaction reagents are arranged at the upper end of the fixing frame; the threaded block moves up and down to compress the corrugated pipe, at the moment, the corrugated pipe can serve as a liquid pump, a reagent in a liquid barrel can be pumped into a test tube by continuously pulling the movable rod up and down, a proper amount of reaction reagent does not need to be indirectly added by manually pinching a rubber head dropper back and forth, the feeding amount of each time can be adjusted, and the tedious weighing step is omitted.

Description

Technical Field

[0001] The invention relates to the technical field of detection kits, in particular to a small and dense low-density lipoprotein cholesterol detection kit. Background Art

[0002] Small dense low-density lipoprotein cholesterol (sdLDL-C) is a special subtype of low-density lipoprotein (LDL) with a smaller molecular weight and higher density. Compared with ordinary LDL, sdLDL-C is more likely to penetrate the blood vessel wall and deposit in the arterial wall, and is therefore closely related to the risk of atherosclerosis and cardiovascular disease.

[0003] When conducting small and dense low-density lipoprotein cholesterol sample experiments and observation experiments, experimenters usually need to use a rubber-tipped dropper to absorb a certain amount of reagent when manually adding reagents and then mix the samples inside the test tube. This usually leads to the following situations: the interns' hands tremble, and when adding reagents, the rubber-tipped dropper will touch the wall of the test tube and make a noise due to nervousness, which may cause damage to the experimental equipment. In addition, the amount of reagent inhaled each time cannot be controlled, so it takes a long time of practice to reach a proficient level. Therefore, an experimental device suitable for early students is needed to solve the problems existing in the background technology. To this end, we propose a small and dense low-density lipoprotein cholesterol detection kit. Summary of the invention

[0004] The purpose of the present invention is to provide a small and compact low-density lipoprotein cholesterol detection kit, which can pump the reagent inside the liquid barrel into the test tube by continuously pulling the movable rod up and down, without manually pinching the rubber-tipped dropper back and forth to indirectly add an appropriate amount of reaction reagent. The feed amount each time can also be adjusted, eliminating the cumbersome weighing steps, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a small and dense low-density lipoprotein cholesterol detection kit, comprising a kit, an inner guard plate is fixedly installed at the inner end of the kit, a temperature changer is fixedly installed at the bottom end of the inner guard plate, a test tube for loading samples is arranged on the inner side of the temperature changer, and the temperature changer can heat or cool the samples in the test tube to simulate the changing state of the samples under different environments, a sealing screw cap for sealing the test tube is rotatably installed on the upper end of the inner guard plate, a guide tube is passed through the upper end of the inner guard plate, and an outer conduit is arranged on the inner side of the guide tube, the right end of the outer conduit is connected to the sealing screw cap, a fixing frame is detachably installed on the inner upper end of the kit, a plurality of dosing devices for adding different reaction reagents are arranged on the upper end of the fixing frame, and an increment device for continuously adding reaction reagents is arranged on the upper end of the inner guard plate;

[0006] The point metering device includes a liquid barrel, which is fixedly installed on the upper end of a fixed frame, a reagent tube is fixedly installed on the inner bottom end of the liquid barrel, a bellows which can change the output liquid volume is fixedly installed on the upper end of the reagent tube, a threaded block is fixedly installed on the upper end of the bellows, and a sleeve is threadedly sleeved on the outer surface of the threaded block, and the rotation of the sleeve can drive the threaded block to move up and down and compress and stretch the bellows, so as to control the volume of the output liquid.

[0007] As a further solution of the present invention, a movable rod is passed through the upper end of the inner guard plate, and the bottom end of the movable rod passes through the upper end of the liquid barrel and is fixedly connected to the upper end of the sleeve. The bottom end of the liquid barrel is fixedly connected to a transfer seat, and a socket is fixedly installed on the upper left end of the transfer seat.

[0008] As a further solution of the present invention, a liquid guiding tube penetrates the inner side of the guide tube, the bottom end of the liquid guiding tube is fixedly connected to a plug, and a sealing ring is fixedly installed at the bottom end of the guide tube, and the sealing ring is fixedly connected to the plug.

[0009] As a further solution of the present invention, the incremental device includes a support frame, which is fixedly mounted on the upper end of the inner guard plate, a connecting sleeve is rotatably mounted on the inner side of the support frame, a liquid box is fixedly mounted on the right end of the connecting sleeve, a rotating sleeve is rotatably mounted on the inner side of the connecting sleeve, and the connecting sleeve can protect the rotating sleeve.

[0010] As a further solution of the present invention, the inner wall of the connecting sleeve is covered with a rubber film, which can increase friction and avoid leakage. A tube head is pierced through the outer surface of the rotating sleeve, and the bottom end of the tube head is connected to an infusion tube.

[0011] As a further solution of the present invention, the infusion tube passes through the outer surface of the outer catheter and is connected to the nozzle, a siphon cavity is opened at the inner end of the rotating sleeve, and a siphon cover is provided on the inner side of the siphon cavity, and the siphon cover is located at the upper end of the tube head.

[0012] As a further solution of the present invention, a transmission gear is rotatably installed at the bottom end of the inner guard plate, and a driving gear is fixedly installed at the rotating connection between the sealing rotary cover and the inner guard plate, the driving gear is meshed with the transmission gear, and a folding clamp is provided at the inner bottom end of the inner guard plate.

[0013] As a further solution of the present invention, an arc block is provided on the inner side of the folding clamp, and the arc block is connected to the folding clamp through a buffer spring. A transmission rack is fixedly installed on one end of the folding clamp close to the transmission gear, and the transmission rack is meshed with the transmission gear.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. When the thread block rotates, the thread block moves up and down and compresses and stretches the bellows to control the volume of the output liquid. When the sleeve stops rotating, it moves up and down to drive the thread block to move up and down. The thread block moves up and down to compress the bellows. At this time, the bellows can act as a liquid pump. The reagent in the liquid barrel can be pumped into the test tube by continuously pulling the movable rod up and down. There is no need to manually pinch the rubber dropper back and forth to indirectly add the appropriate amount of reaction reagent. The feed amount each time can also be adjusted, eliminating the cumbersome weighing steps;

[0016] 2. However, when it is necessary to continuously add reagents or react with solutions, the liquid box is rotated to be in a vertical state, and the liquid outlet is directly opposite to the upper side of the siphon cavity. The liquid box is pressed downward intermittently, so that the siphon cover inside the siphon cavity is first covered on the outer surface of the tube head. When it rises again, negative pressure will be generated, and the siphon cover will move upward. At this time, a siphon phenomenon will occur, so that the liquid inside the liquid box will eventually flow into the test tube through the infusion tube through the siphon phenomenon. When it is not necessary to continuously add reagents, the liquid box can be directly restored to its initial state to directly disconnect the liquid addition step. In this way, there is no need to use a syringe to add reagents, and there will be no leakage or spilling of liquid.

[0017] 3. The driving gear on the sealing screw cap first drives the transmission gear to rotate, and the transmission rack moves left and right under the action of meshing with the transmission gear. The transmission gear drives the folding clamp to stretch, so that the gap at the center of the folding clamp gradually decreases, and drives the arc block to clamp the test tube through the buffer spring, thereby achieving a stable effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A schematic diagram of the structure of a small and dense low-density lipoprotein cholesterol detection kit;

[0019] Figure 2 A schematic diagram of the internal structure of a small and compact low-density lipoprotein cholesterol detection kit;

[0020] Figure 3 It is the cross-section of the inner guard plate;

[0021] Figure 4 It is a structural schematic diagram of a point measuring device;

[0022] Figure 5 for Figure 4 Schematic diagram of the structure at A in the middle;

[0023] Figure 6 It is a structural schematic diagram of the cross section of a liquid barrel;

[0024] Figure 7 is a schematic diagram of the structure of the incremental device;

[0025] Figure 8 is a cross-sectional view of the connecting sleeve;

[0026] Fig. 9 This is a disassembled diagram of the increment device.

[0027] In the figure: 1. reagent box; 2. inner guard plate; 3. temperature changer; 4. transmission gear; 5. transmission rack; 6. buffer spring; 7. folding clamp; 8. test tube; 11. nozzle;

[0028] 101, sealing screw cap; 102, external catheter; 103, guide tube; 104, liquid guide tube; 105, sealing ring; 106, plug;

[0029] 201, liquid box; 202, connecting sleeve; 203, infusion tube; 204, return spring; 205, support frame; 206, rotating sleeve; 207, tube head; 208, siphon chamber; 209, siphon cover;

[0030] 301. movable rod; 302. liquid barrel; 303. fixing bracket; 304. adapter; 305. threaded block; 306. sleeve; 307. bellows; 308. reagent tube; 309. one-way valve; 310. sealing ball; 311. socket; 312. sealing spring. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1: Please refer to Figure 1 ,2 , 3, 4, a small and compact low-density lipoprotein cholesterol detection kit, comprising a kit 1, an inner guard plate 2 is fixedly installed at the inner end of the kit 1, a temperature changer 3 is fixedly installed at the bottom end of the inner guard plate 2, a test tube 8 for loading a sample is arranged on the inner side of the temperature changer 3, and the temperature changer 3 can heat or cool the sample in the test tube 8 to simulate the changing state of the sample under different environments, a sealing screw cap 101 for sealing the test tube 8 is rotatably installed on the upper end of the inner guard plate 2, a guide tube 103 is penetrated at the upper end of the inner guard plate 2, and an outer guide tube 102 is arranged on the inner side of the guide tube 103;

[0033] The right end of the outer conduit 102 is connected to the sealing screw cap 101. The upper inner end of the reagent kit 1 is detachably mounted with a fixing frame 303. The upper end of the fixing frame 303 is provided with a plurality of dosing devices for adding different reaction reagents. The upper end of the inner guard plate 2 is provided with an increment device for continuously adding reaction reagents.

[0034] See also Figure 4 , 5 6. The dot metering device includes a liquid barrel 302, which is fixedly mounted on the upper end of a fixed frame 303. A reagent tube 308 is fixedly mounted on the inner bottom end of the liquid barrel 302. A bellows 307 capable of changing the output liquid volume is fixedly mounted on the upper end of the reagent tube 308. A threaded block 305 is fixedly mounted on the upper end of the bellows 307. A sleeve 306 is threadedly sleeved on the outer surface of the threaded block 305. The sleeve 306 can be rotated to drive the threaded block 305 to move up and down and compress and stretch the bellows 307, so as to control the volume of the output liquid. Specifically, the dot metering device is arranged in a ring shape with the guide tube 103 as the center, so that the guide tube 103 can more conveniently reach the dot metering device in any direction. The bottom end of the sealing screw cap 101 is fixedly connected to the nozzle 11.

[0035] Example 2: Please refer to Figure 5 , 6 , a small and compact low-density lipoprotein cholesterol detection kit, based on Example 1, the upper end of the inner guard plate 2 is penetrated with a movable rod 301, and the bottom end of the movable rod 301 passes through the upper end of the liquid barrel 302 and is fixedly connected to the upper end of the sleeve 306, the bottom end of the liquid barrel 302 is fixedly connected with an adapter 304, and the upper left end of the adapter 304 is fixedly installed with a socket 311;

[0036] Specifically, when the sleeve 306 stops rotating, the subsequent up and down movement can drive the threaded block 305 to move up and down, and the up and down movement of the threaded block 305 can compress the bellows 307. At this time, the bellows 307 can act as a liquid pump. A one-way valve 309 is fixedly installed at the bottom end of the reagent tube 308, and the one-way valve 309 can only allow the reaction reagent to enter the reagent tube 308 and the bellows 307. When the bellows 307 is compressed, the internal reagent will not be able to flow out from the one-way valve 309. A sealing ball 310 is arranged on the inner side of the socket 311, and the sealing ball 310 is connected to the bottom end of the inner side of the socket through a sealing spring 312, so that the sealing ball 310 can always abut against the liquid outlet hole at the upper end of the socket 311, and the outer surface of the sealing ball 310 and the contact surface between the hole 311 and the sealing ball 310 are frosted, so that the sealing performance is further improved to prevent the reagent from flowing out;

[0037] See also Figure 4 , 5 6. A liquid guide tube 104 runs through the inner side of the guide tube 103. The bottom end of the liquid guide tube 104 is fixedly connected to a plug 106, and a sealing ring 105 is fixedly installed at the bottom end of the guide tube 103. The sealing ring 105 is fixedly connected to the plug 106. Specifically, one end of the liquid guide tube 104 close to the sealing screw cap 101 is connected to the nozzle 11. The liquid inlet of the plug 106 is located on the outer surface of the non-bottom end, and the bottom end is an arc-shaped groove, which makes it easier to press the sealing ball 310 downward. More specifically, a pointer is fixedly installed on the upper end of the guide tube 103, and when the guide tube 103 is rotated so that the pointer points to the upper end of any movable rod 301, it means that the current reagent is selected;

[0038] See also Figure 7 , 8 9. The increment device includes a support frame 205, which is fixedly mounted on the upper end of the inner guard plate 2. A connecting sleeve 202 is rotatably mounted on the inner side of the support frame 205. A liquid box 201 is fixedly mounted on the right end of the connecting sleeve 202. A rotating sleeve 206 is rotatably mounted on the inner side of the connecting sleeve 202, and the connecting sleeve 202 can protect the rotating sleeve 206. The inner wall of the connecting sleeve 202 is covered with a rubber film, which can increase the friction and avoid leakage. A pipe head 207 is penetrated on the outer surface of the rotating sleeve 206, and the bottom end of the pipe head 207 is connected with an infusion tube 203, which passes through the outer surface of the outer catheter 102 and is connected with the nozzle 11. A siphon cavity 208 is opened at the inner end of the rotating sleeve 206, and a siphon cover 209 is provided on the inner side of the siphon cavity 208, and the siphon cover 209 is located at the upper end of the pipe head 207;

[0039] Specifically, a liquid outlet is provided at the left end of the liquid box 201, and a limit block is provided on the inner side of the support frame 205, so that the liquid box 201 can directly maintain a vertical state when rotating. The support frame 205 is connected to the connecting sleeve 202 by a return spring 204. When the liquid box 201 is in a vertical state, the liquid outlet is directly opposite to the upper side of the siphon cavity 208, and the liquid box 201 is pressed downward intermittently, so that the siphon cover 209 inside the siphon cavity 208 is first sleeved on the outer surface of the tube head 207, and negative pressure is generated when it rises again, and the siphon cover 209 moves upward, and a siphon phenomenon is generated at this time, so that the liquid inside the liquid box 201 finally flows into the test tube 8 through the infusion tube 203 through the siphon phenomenon;

[0040] See also Figure 3 A transmission gear 4 is rotatably installed at the bottom end of the inner guard plate 2, and a driving gear is fixedly installed at the rotating connection between the sealing rotary cover 101 and the inner guard plate 2, and the driving gear is meshed with the transmission gear 4. A folding clamp 7 is provided at the inner bottom end of the inner guard plate 2, and an arc block is provided inside the folding clamp 7, and the connection between the arc block and the folding clamp 7 is connected by a buffer spring 6. A transmission rack 5 is fixedly installed at one end of the folding clamp 7 close to the transmission gear 4, and the transmission rack 5 is meshed with the transmission gear 4.

[0041] The working principle of the present invention is:

[0042] When in use, the sealing screw cover 101 is turned outward, and the test tube 8 containing the sample is placed in the inner guard plate 2, and then the sealing screw cover 101 is closed. When the sealing screw cover 101 rotates, the driving gear on the sealing screw cover 101 first drives the transmission gear 4 to rotate, and the transmission rack 5 moves left and right under the meshing action with the transmission gear 4, and the transmission gear 4 drives the folding clamp 7 to stretch, so that the gap at the center of the folding clamp 7 is gradually reduced, and the arc block is driven by the buffer spring 6 to clamp the test tube 8, so as to achieve a stable effect;

[0043] Then, the temperature changer 3 is used to simulate the change of the environment inside the test tube 8, so as to complete the results of various experiments, and finally the state of the sample is analyzed according to the simulated structure. During the experiment, various reaction solvents or chromosomes need to be added to the sample to achieve the state of the sample analysis. At this time, the reagents to be added are screened according to the required conditions, and the guide tube 103 is rotated so that the pointer points to the corresponding movable rod 301, and then the guide tube 103 is pressed downward to allow the plug 106 to be inserted into the socket 311;

[0044] Then, according to the experimental requirements, the required dosage of the reagent is added, the movable rod 301 is rotated, the sleeve 306 is rotated and drives the threaded block 305 to rotate. When the threaded block 305 rotates, the threaded block 305 moves up and down and compresses and stretches the bellows 307, so as to control the volume of the output liquid. When the sleeve 306 stops rotating, it then moves up and down to drive the threaded block 305 to move up and down. The threaded block 305 moves up and down to compress the bellows 307. At this time, the bellows 307 can act as a liquid pump. The movable rod 301 is continuously pulled up and down to realize the pumping of the reagent inside the liquid barrel 302 into the test tube 8. There is no need to manually pinch the rubber-tipped dropper back and forth to indirectly add an appropriate amount of reaction reagent, and the feeding amount each time can be adjusted, which saves the cumbersome weighing steps.

[0045] However, when it is necessary to continuously add reagents or reaction solutions, the liquid box 201 is rotated to be in a vertical state, and the liquid outlet is directly opposite to the upper side of the siphon cavity 208. The liquid box 201 is pressed downward intermittently, so that the siphon cover 209 inside the siphon cavity 208 is first sleeved on the outer surface of the tube head 207. When it rises again, negative pressure will be generated, and the siphon cover 209 will move upward. At this time, a siphon phenomenon will occur, so that the liquid inside the liquid box 201 will eventually flow into the test tube 8 through the infusion tube 203 through the siphon phenomenon. When it is not necessary to continuously add reagents, the liquid box 201 can be directly restored to its initial state to directly disconnect the liquid adding step. In this way, there is no need to use a syringe to add reagents, and there will be no leakage or spilling of liquid.

[0046] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed in the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A small and dense low-density lipoprotein cholesterol detection kit, comprising a kit (1), characterized in that: The inner end of the reagent box (1) is fixedly mounted with an inner protective plate (2), the bottom end of the inner protective plate (2) is fixedly mounted with a temperature changer (3), the inner side of the temperature changer (3) is provided with a test tube (8) for loading a sample, and the temperature changer (3) can heat or cool the sample in the test tube (8) to simulate the changing state of the sample under different environments, the upper end of the inner protective plate (2) is rotatably mounted with a sealing screw cap (101) for sealing the test tube (8), and the inner protective plate ( 2) is provided with a guide tube (103) at the upper end thereof, and an outer conduit (102) is provided inside the guide tube (103), and the right end of the outer conduit (102) is connected to the sealing screw cap (101), a fixing frame (303) is detachably mounted on the inner upper end of the reagent box (1), and a plurality of dosing devices for adding different reaction reagents are provided on the upper end of the fixing frame (303), and an increment device for continuously adding reaction reagents is provided on the upper end of the inner guard plate (2); The dot metering device comprises a liquid barrel (302), wherein the liquid barrel (302) is fixedly mounted on the upper end of a fixed frame (303), a reagent tube (308) is fixedly mounted on the inner bottom end of the liquid barrel (302), a bellows (307) capable of changing the output liquid volume is fixedly mounted on the upper end of the reagent tube (308), a threaded block (305) is fixedly mounted on the upper end of the bellows (307), and a sleeve (306) is threadedly sleeved on the outer surface of the threaded block (305), and the sleeve (306) can be rotated to drive the threaded block (305) to move up and down and compress and stretch the bellows (307), thereby controlling the volume of the output liquid.

2. A small and dense low-density lipoprotein cholesterol detection kit according to claim 1, characterized in that: A movable rod (301) is passed through the upper end of the inner guard plate (2), and the bottom end of the movable rod (301) passes through the upper end of the liquid barrel (302) and is fixedly connected to the upper end of the sleeve (306). The bottom end of the liquid barrel (302) is fixedly connected to a transfer seat (304), and a socket (311) is fixedly installed on the upper left end of the transfer seat (304).

3. A small and dense low-density lipoprotein cholesterol detection kit according to claim 2, characterized in that: A liquid guiding tube (104) penetrates the inner side of the guide tube (103), the bottom end of the liquid guiding tube (104) is fixedly connected to a plug (106), and a sealing ring (105) is fixedly installed at the bottom end of the guide tube (103), and the sealing ring (105) is fixedly connected to the plug (106).

4. A small and dense low-density lipoprotein cholesterol detection kit according to claim 1, characterized in that: The increment device comprises a support frame (205), wherein the support frame (205) is fixedly mounted on the upper end of the inner guard plate (2), a connecting sleeve (202) is rotatably mounted on the inner side of the support frame (205), a liquid box (201) is fixedly mounted on the right end of the connecting sleeve (202), a rotating sleeve (206) is rotatably mounted on the inner side of the connecting sleeve (202), and the connecting sleeve (202) can protect the rotating sleeve (206).

5. A small and dense low-density lipoprotein cholesterol detection kit according to claim 4, characterized in that: The inner wall of the connecting sleeve (202) is covered with a rubber film, which can increase friction and prevent leakage. The outer surface of the rotating sleeve (206) is provided with a tube head (207), and the bottom end of the tube head (207) is connected to the infusion tube (203).

6. A small and dense low-density lipoprotein cholesterol detection kit according to claim 5, characterized in that: The infusion tube (203) passes through the outer surface of the outer catheter (102) and is connected to the nozzle (11). A siphon cavity (208) is provided at the inner end of the rotating sleeve (206), and a siphon cover (209) is provided inside the siphon cavity (208). The siphon cover (209) is located at the upper end of the tube head (207).

7. A small and dense low-density lipoprotein cholesterol detection kit according to claim 1, characterized in that: A transmission gear (4) is rotatably mounted on the bottom end of the inner guard plate (2), and a driving gear is fixedly mounted on the rotation connection between the sealing rotary cover (101) and the inner guard plate (2), the driving gear meshing with the transmission gear (4), and a folding clamp (7) is provided on the inner bottom end of the inner guard plate (2).

8. A small and dense low-density lipoprotein cholesterol detection kit according to claim 7, characterized in that: An arc block is arranged inside the folding clamp (7), and the connection between the arc block and the folding clamp (7) is connected via a buffer spring (6). A transmission rack (5) is fixedly mounted on one end of the folding clamp (7) close to the transmission gear (4), and the transmission rack (5) is meshed with the transmission gear (4).

Images