A method for introducing and cultivating Korean pine

Abstract

The present invention relates to the technical field related to the introduction and cultivation of Korean pine, and discloses a method for introducing and cultivating Korean pine. By circumcising the rootstock and applying a special gel, the nutrients and hormones in the rootstock are guided into the gel, and then grafting or cutting is carried out based on the gel. Compared with the traditional cross-cut grafting method, the circumcision adopted in this scheme can enrich the nutrients of the rootstock to the greatest extent, avoid the competition for nutrients between the branches and leaves of the rootstock and the scion, and at the same time, the gel also has good wrapping properties and can completely wrap the cut of the scion. Compared with the approximate docking of the cortex of the traditional grafting rootstock and the cut of the scion, the interface is more neat, and the scion can obtain more sufficient nutrients, which can greatly improve the survival rate of the scion. Compared with the traditional cutting method, in this scheme, the scion is inserted into the gel in advance, and the scion can continuously obtain sufficient nutrients during the rooting process, so that the cutting survival rate is greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of introduction and cultivation of red pine, in particular to a method for introduction and cultivation of red pine. Background Art

[0002] Korean pine, also known as fruit pine and Korean pine, is a member of the Pinaceae family and an evergreen five-needle pine tree species. Natural Korean pine can be up to 40m tall and have a breast diameter of more than 2m. It is a common tree species in the Northeast forest area. The large-diameter Korean pine wood has excellent physical properties and is a good timber species for industry and household use. Korean pine is strong and abundant, and because Korean pine seeds are rich in oil, large in size, have a good taste and have certain therapeutic effects, there is a large social demand for it at home and abroad, and it has a high economic value. Therefore, in the national economic construction, Korean pine not only provides timber and protects the ecology, but has also become an important economic source for many foresters in the Northeast forest area. However, due to the wide range of uses of Korean pine wood, Korean pine has been over-logged. The natural Korean pine forest has been almost completely cut down, and the artificially created Korean pine forest requires a longer period of time. Asexual reproduction technology is an effective way to solve this problem.

[0003] However, due to the slow growth and development of red pine, the existing introduction and cultivation methods, including grafting and cuttings, have a low survival rate. The alignment of the interface during grafting is difficult to accurately control, the efficiency of nutrient delivery is limited, and it is easily disturbed by pests and diseases. Cutting propagation relies entirely on the nutrients stored in the branches themselves, resulting in insufficient nutrients and a low rooting rate. Summary of the invention

[0004] The purpose of the present invention is to provide a method for introducing and cultivating Korean pine to solve the problem of low survival rate of the current traditional introduction and cultivation method proposed in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for introducing and cultivating Korean pine, comprising the following steps:

[0006] S1. Rootstock selection and treatment: First, select a healthy and pest-free Scots pine as the rootstock. Before grafting, prune the rootstock to remove weak, diseased and overcrowded branches. At the same time, gird the rootstock using a sharp knife to gird the cortex of the rootstock and remove the bark.

[0007] S2. Preparation of gel matrix: The gel components include chitosan, sodium alginate, antibacterial agent, rooting promoter, cross-linking agent, humectant, and the balance is pH regulator and water. The above raw materials are fully mixed and configured into a gel;

[0008] S3. Scion selection and processing: Select healthy, disease- and insect-free branches from the Korean pine mother tree as scion. Cut the bottom of the scion diagonally and remove excess leaves.

[0009] S4. Grafting preparation: First, spray a growth inhibitor on the circumcision incision of the prepared rootstock to inhibit the cortical healing at the incision of the rootstock. Then, wrap the incision of the rootstock with a special grafting strip, inject the prepared gel into the grafting strip, and evacuate the air to make the gel completely cover the incision, ensuring that the gel is in complete contact with the cortex at both ends of the incision. After that, let it stand for 24 hours until the sap of the rootstock is completely fused with the gel, so that the cortex at both ends of the circumcision incision can exchange and transport water and nutrients with the help of the gel.

[0010] S5. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortices of the circumcision incision of the rootstock, and seal it with plastic wrap.

[0011] S6. Rooting and transplanting: After the scion is inserted into the gel, it survives by means of the nutrients in the sap of the rootstock immersed in the gel. After a period of cultivation, the scion will take root and grow new branches and leaves in the gel. When the roots grow to 3 - 4 cm, the scion can be removed and transplanted into the soil for subsequent transplantation.

[0012] Further, the depth of the circumcision incision in S1 reaches the cortex but does not damage the xylem, and the width of the incision is between 1 cm and 2 cm.

[0013] Further, the gel ratio is 10% chitosan, 8% sodium alginate, 0.5% antibacterial agent, 0.05% rooting promoter, 0.5% crosslinking agent, 3% moisturizing agent, and the balance is water. The PH regulator is based on the amount required to adjust the PH to a reasonable range.

[0014] Further, the preparation method of the gel matrix includes the following steps:

[0015] S201. Preparation of chitosan solution: Slowly add the weighed chitosan powder to warm water and stir while adding until a uniform suspension is formed. The amount of water is 5 times the mass of chitosan, and the water temperature is controlled at 50°C.

[0016] S202. Preparation of sodium alginate solution: Dissolve the weighed sodium alginate powder in warm water at 60°C and stir while adding until a transparent and viscous solution is formed in the warm water.

[0017] S203. Mixing and stirring: Slowly pour the dissolved chitosan solution into the sodium alginate solution, and continuously stir with a stirrer to avoid agglomeration. Then, sequentially add the accurately weighed antibacterial agent, rooting promoter, crosslinking agent, and moisturizing agent, and stir well after each addition.

[0018] S204. pH adjustment: Measure the pH value of the mixed solution using a pH meter, and add a pH regulator as needed to adjust the pH to be consistent with the pH of the rootstock sap.

[0019] S205. Filtration and sterilization: Filter the mixed solution using a filter to remove undissolved impurities, and then heat the filtered solution to 80 °C in a water bath and maintain it for 30 minutes for high-temperature sterilization treatment to ensure a sterile state.

[0020] S206. Solidification and molding: Let the sterilized mixed solution stand and cool to room temperature to solidify it into a gel state.

[0021] Further, in S204, the pH of the gel is finally adjusted to be between 5.5 and 6.5.

[0022] Further, in S3, the length of the scion is controlled to be between 9 and 12 cm and has at least 2 to 3 bud eyes.

[0023] Further, in S3, the grafting wrapping strip is made of flexible rubber material, and a semi-circular glue injection groove is provided inside. The glue injection groove is used to hold the gel, and an insertion port is provided above the glue injection groove. The scion is inserted into the gel through the insertion port.

[0024] Further, the grafting wrapping strip surrounds the annular incision of the rootstock and is fixed to the rootstock with double-sided adhesive tape.

[0025] Further, in S5, when the scion is inserted into the gel, the lower end of the scion has no direct contact with the rootstock incision.

[0026] Further, when transplanting in S6, the gel and the root system of the scion are taken out together for transplantation to avoid damaging the root system.

[0027] Compared with the prior art, the beneficial effects of the present invention are:

[0028] A cultivation method for introduced Korean pine involves making a circumferential cut on the rootstock and applying a special gel, thereby guiding the nutrients and hormones in the rootstock into the gel. Then, grafting or cutting is carried out based on the gel. Compared with the traditional horizontal cutting grafting method, the circumferential cut adopted in this solution can enrich the nutrients of the rootstock to the greatest extent, avoiding the competition for nutrients between the branches and leaves of the rootstock and the scion. At the same time, the gel also has good wrapping properties and can completely wrap the cut of the scion. Compared with the approximate docking of the cortex of the traditional grafting rootstock and the cut of the scion, the interface is neater, and the scion can obtain more sufficient nutrients, which can greatly improve the survival rate of the scion. Compared with the traditional cutting method, in this solution, the scion is inserted into the gel, and the scion can continuously obtain sufficient nutrients during the rooting process, resulting in a significant increase in the cutting survival rate. Moreover, compared with the artificially prepared cutting substrate, the nutrients of the gel in this solution come from the rootstock and also contain various biological hormones and unknown active substances, which are more complete than the artificial substrate and are more conducive to cutting rooting. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the cultivation method of the present invention;

[0030] Figure 2 It is a schematic diagram of the grafting wrapping structure of the present invention.

[0031] Reference numerals in the figure: 1, grafting wrapping strip; 101, glue injection groove; 102, insertion port; 2, double-sided adhesive strip. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0033] To further understand the content of the present invention, the present invention will be described in detail with reference to the accompanying drawings.

[0034] Example 1: A cultivation method for introduced Korean pine includes the following steps:

[0035] I. Selection and treatment of the rootstock: First, select healthy and disease-free Scotch pine as the rootstock, ensuring that the rootstock has well-developed roots and can stably absorb and transport nutrients. Before grafting, prune the rootstock to remove weak branches, diseased branches, and overcrowded branches to reduce nutrient consumption and improve the ventilation and light transmission of the grafting part. At the same time, perform a circumferential cut on the rootstock. Use a sharp tool to make a circumferential cut on the cortex of the rootstock and remove the bark. The cutting depth reaches the cortex but does not damage the xylem. The cutting width is between 1 cm and 2 cm, and ensure that the cut is smooth and flat, which is conducive to the attachment of the gel and the absorption of nutrients.

[0036] The circumcision operation completely cuts off the nutrient and water transportation channels of the Scotch pine acting as the rootstock, causing nutrients and water to accumulate at the circumcision site and gradually integrate into the gel. Compared with the traditional cross-cutting method, the interception efficiency of nutrients is higher, which is conducive to competing with the original branches and leaves of the scion and rootstock for nutrients, and can improve the survival rate and growth rate of the scion.

[0037] II. Preparation of gel matrix: The gel components include 10% chitosan, 8% sodium alginate, 0.5% antibacterial agent, 0.05% rooting promoter, 0.5% cross-linking agent, 3% humectant, and the balance is pH regulator and water.

[0038] Among them, chitosan, as the main matrix, has good adsorption, film-forming and permeability, facilitating the enrichment and transfer of nutrients.

[0039] Sodium alginate has good film-forming properties and can form a stable three-dimensional network structure film after cross-linking with calcium ions and the like. This film can not only effectively protect the grafting site from external mechanical damage and pathogen invasion, but also maintain a moist environment at the incision, which is conducive to cell regeneration and healing.

[0040] The rooting promoter can stimulate the root growth of the scion and accelerate the formation and development of roots. This is crucial for improving the stress resistance and growth rate of the scion, and helps the scion to adapt to the new growth environment and resume normal growth as soon as possible.

[0041] The cross-linking agent plays a role in connecting matrix molecules in the gel and can form a stable gel network structure, which helps to improve the mechanical properties and water resistance of the gel, enabling the gel to maintain a stable shape and performance during the operation.

[0042] The humectant can absorb and retain a certain amount of water, providing a continuous moist environment for the grafting site, which is crucial for maintaining cell activity and promoting the healing process.

[0043] The pH regulator is used to adjust the pH value of the gel to make it as close as possible to the pH of the sap that transports nutrients in the rootstock, which helps to maintain the biological activity of the sap.

[0044] The preparation method of the gel matrix includes the following steps:

[0045] 1. Preparation of chitosan solution: Slowly add the weighed chitosan powder to warm water, stirring while adding until a uniform suspension is formed. The amount of water is 5 times the mass of chitosan, and the water temperature is controlled at 50°C.

[0046] 2. Preparation of sodium alginate solution: Dissolve the weighed sodium alginate powder in warm water at 60°C, also stirring while adding until a transparent viscous solution is formed in the warm water.

[0047] 3. Mixing and stirring: Slowly pour the dissolved chitosan solution into the sodium alginate solution while continuously stirring with a stirrer to avoid lumping. Then, sequentially add accurately weighed antibacterial agents, rooting promoters, crosslinking agents, and humectants, and fully stir evenly after each addition.

[0048] 4. pH adjustment: Use a pH meter to measure the pH value of the mixed solution and add a pH regulator as needed to adjust the pH to between 5.5 and 6.5, which is consistent with the sap pH of the Korean pine tree, facilitating the transport of nutrients.

[0049] 5. Filtration and sterilization: Filter the mixed solution using a filter to remove undissolved impurities, and then heat the filtered solution to 80°C in a water bath and maintain it for 30 minutes for high-temperature sterilization to ensure a sterile state.

[0050] 6. Solidification and molding: Let the sterilized mixed solution stand and cool to room temperature to solidify it into a gel.

[0051] III. Scion selection and treatment: Select branches that are robust in growth, have plump bud eyes, and are free of pests and diseases from the Korean pine mother tree as scions. The length of the scions is controlled between 9 - 12 cm and they should have at least 2 - 3 bud eyes. Cut the bottom of the scion obliquely and remove the extra leaves to reduce water evaporation and nutrient consumption.

[0052] IV. Grafting preparation: First, spray a growth inhibitor on the circumcision incision of the prepared rootstock to inhibit the cortical healing at the rootstock incision, so that the rootstock incision is exposed to the gel for a longer time, increasing the effective retention time of the gel and providing a longer guarantee time for the rooting of the scion. Then, use a special grafting wrapping strip 1 to wrap the rootstock incision, and then inject the prepared gel into the grafting wrapping strip 1, and drain the air to make the gel completely cover the incision, ensuring that the gel is in full contact with the cortex at both ends of the incision. After that, let it stand for 24 hours until the sap of the rootstock and the gel are completely fused, so that the cortex at both ends of the circumcision incision can exchange and transport water and nutrients with the help of the gel.

[0053] V. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortices of the circumcision incision of the rootstock, and the lower end of the scion does not contact the rootstock incision, and seal the insertion opening with plastic wrap.

[0054] VI. Rooting and transplantation: After the scion is inserted into the gel, it survives by relying on the nutrients in the sap of the rootstock immersed in the gel. After a period of cultivation, the scion will root and grow new branches and leaves in the gel. When the roots grow to 3 - 4 cm, the scion can be removed and transplanted into the soil for subsequent transplantation. When transplanting, take out the gel and the scion roots together to avoid damaging the roots.

[0055] Example 2: A method for introducing and cultivating Korean pine. When it is necessary to quickly achieve economic benefits, grafting can be adopted, which specifically includes the following steps:

[0056] I. Selection and treatment of rootstock: First, select Pinus sylvestris var. mongolica with strong growth and no pests and diseases as the rootstock, ensuring that the rootstock has well-developed roots and can stably absorb and transport nutrients. Before grafting, prune the rootstock to remove weak branches, diseased branches, and overcrowded branches to reduce nutrient consumption and improve ventilation and light transmission of the grafted part. At the same time, perform a circumcision treatment on the rootstock. Use a sharp tool to circumcise the cortex of the rootstock and remove the bark. The incision depth reaches the cortex but does not damage the xylem. The incision width is between 0.5 - 1 cm, and ensure that the incision is smooth and flat, which is conducive to the attachment of the gel and the absorption of nutrients;

[0057] II. Preparation of gel matrix: The gel components include 10% chitosan, 8% sodium alginate, 0.5% antibacterial agent, 0.5% cross-linking agent, 3% moisturizing agent, and the balance is pH regulator and water.

[0058] Compared with Example 1, the rooting promoter is cancelled to avoid the rooting of the scion, so that the scion can grow and fuse with the incision.

[0059] The preparation method of the gel matrix includes the following steps:

[0060] 1. Preparation of chitosan solution: Slowly add the weighed chitosan powder to warm water and stir while adding until a uniform suspension is formed. The amount of water is 5 times the mass of chitosan, and the water temperature is controlled at 50°C.

[0061] 2. Preparation of sodium alginate solution: Dissolve the weighed sodium alginate powder in warm water at 60°C and also stir while adding until a transparent viscous solution is formed in the warm water.

[0062] 3. Mixing and stirring: Slowly pour the dissolved chitosan solution into the sodium alginate solution, and continuously stir with a stirrer to avoid agglomeration. Then, sequentially add the accurately weighed antibacterial agent, rooting promoter, cross-linking agent, and moisturizing agent, and stir well after each addition.

[0063] 4. pH adjustment: Use a pH meter to measure the pH value of the mixed solution and add a pH regulator as needed to adjust the pH to between 5.5 and 6.5, which is consistent with the sap pH of Korean pine, facilitating the transportation of nutrients.

[0064] 5. Filtration and sterilization: Filter the mixed solution using a filter to remove undissolved impurities, and then heat the filtered solution to 80°C in a water bath and hold for 30 minutes for high-temperature sterilization to ensure a sterile state.

[0065] 6. Solidification and Molding: Let the sterilized mixture stand and cool to room temperature until it solidifies into a gel.

[0066] III. Scion Selection and Treatment: Select branches that are robust in growth, have plump bud eyes, and are free of pests and diseases from the Korean pine mother tree as scions. The length of the scions is controlled between 9 - 12 cm and should have at least 2 - 3 bud eyes. Obliquely cut the bottom of the scion and remove the excess leaves to reduce water evaporation and nutrient consumption.

[0067] IV. Grafting Preparation: First, use a special grafting wrapping strip 1 to wrap the cut of the rootstock. Then, inject the prepared gel into the grafting wrapping strip 1 and expel the air, so that the gel completely covers the cut, ensuring that the gel is in full contact with the cortex at both the upper and lower ends of the cut. After that, let it stand for 24 hours until the sap of the rootstock and the gel are completely fused, enabling the cortex at both the upper and lower ends of the circumcision cut to exchange and transport water and nutrients with the help of the gel.

[0068] V. Indirect Grafting: After the gel is prepared, insert the scion prepared in S3 into the gel, making the end of the scion located between the upper and lower cortices of the circumcision cut of the rootstock, and pressing the end of the scion tightly against the upper or lower cortex of the circumcision cut of the rootstock. Seal the insertion opening with plastic wrap. After the scion is inserted into the gel, it survives by relying on the nutrients in the sap of the rootstock immersed in the gel. After a period of cultivation, the upper and lower cortices at the circumcision of the rootstock grow and fuse together, and the scion is also fused inside, achieving the intensive grafting of the scion.

[0069] The grafting wrapping strip 1 used in the above-mentioned Embodiment 1 and Embodiment 2 is made of flexible rubber material, with a semi-circular glue injection groove 101 inside. The glue injection groove 101 is used to hold the gel. Above the glue injection groove 101, there is an insertion opening 102, which is also the injection port for the gel. At the same time, the scion is inserted into the gel through the insertion opening 102. The grafting wrapping strip 1 surrounds the circular cut of the rootstock and is fixed on the rootstock by a double-sided adhesive strip 2 to ensure sealing. The grafting wrapping strip 1 specifically provides fixation for the gel, reducing the difficulty of gel wrapping and minimizing the pollution of the gel by the external environment.

[0070] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for introducing and cultivating Korean pine, characterized in that: The following steps are involved: S1. Rootstock selection and treatment: First, select a healthy and pest-free Scots pine as the rootstock. Before grafting, prune the rootstock to remove weak, diseased and overcrowded branches. At the same time, gird the rootstock using a sharp knife to gird the cortex of the rootstock and remove the bark. S2. Preparation of gel matrix: The gel components include chitosan, sodium alginate, antibacterial agent, rooting promoter, cross-linking agent, humectant, and the balance is pH regulator and water. The above raw materials are fully mixed and configured into a gel; S3. Scion selection and processing: Select healthy, full-bud, disease-free and insect-free branches from Korean pine mother trees as scions, cut the bottom of the scion diagonally and remove excess leaves; S4. Grafting preparation: First, spray a growth inhibitor on the prepared rootstock ring cut to inhibit the healing of the cortex at the rootstock ring cut, then use a special grafting strip (1) to wrap the rootstock ring cut, and then inject the prepared gel into the grafting strip (1), and evacuate the air, so that the gel completely covers the cut, ensuring that the gel is in full contact with the cortex at the upper and lower ends of the cut, and then let it stand for 24 hours until the sap of the rootstock and the gel are completely fused, so that the cortex at the upper and lower ends of the ring cut can exchange and transport water and nutrients with the help of the gel; S5. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortex of the rootstock ring cut, and seal it with plastic wrap; S6. Rooting and transplanting: After the scion is inserted into the gel, it survives with the help of nutrients in the rootstock sap immersed in the gel. After a period of maintenance, the scion will take root in the gel and grow new branches and leaves. When the root system grows to 3-4cm, the scion can be removed and transplanted into the soil for subsequent transplanting.

2. The method for introducing and cultivating Korean pine planting according to claim 1, characterized in that: The depth of the circumcision in S1 reaches the cortex but does not damage the xylem, and the width of the incision is between 1 cm and 2 cm.

3. A method for introducing and cultivating Korean pine, according to claim 1, characterized in that: The gel ratio is 10% chitosan, 8% sodium alginate, 0.5% antibacterial agent, 0.05% rooting promoter, 0.5% cross-linking agent, 3% moisturizing agent, and the balance is water. The pH regulator is based on the amount required to adjust the pH to a reasonable range.

4. A method for introducing and cultivating Korean pine planting, as described in claim 1, characterized in that: The preparation method of the gel matrix comprises the following steps: S201 chitosan solution preparation: The weighed chitosan powder was slowly added to warm water, stirring while adding until a uniform suspension was formed, the amount of water was 5 times the mass of chitosan, and the water temperature was controlled at 50 ° C; S202. Preparation of sodium alginate solution: The weighed sodium alginate powder was dissolved in warm water at 60 ° C, and the mixture was stirred until a transparent viscous solution was formed in warm water; S203. Mixing and stirring: The dissolved chitosan solution is slowly poured into the sodium alginate solution, and stirred continuously with a stirrer to avoid agglomeration, and then the precisely weighed antibacterial agent, rooting promoter, cross-linking agent and moisturizing agent are added successively, and each addition is thoroughly stirred; S204. PH adjustment: Use a PH meter to measure the pH value of the mixed solution, and add a PH regulator as needed to adjust the pH to the same pH as the rootstock sap; S205. Filtration and sterilization: Filter the mixture using a filter to remove undissolved impurities, and then heat the filtered solution to 80 °C in a water bath and maintain it for 30 minutes for high-temperature sterilization to ensure a sterile state; S206. Solidification and molding: Let the sterilized mixture stand and cool to room temperature to solidify it into a gel.

5. A method for introducing and cultivating Korean pine, according to claim 4, characterized in that: In S204, the pH of the gel is finally adjusted to between 5.5 and 6.

5.

6. A method for introducing and cultivating Korean pine, according to claim 1, characterized in that: In S3, the length of the scion is controlled to be between 9 and 12 cm and has at least 2 to 3 bud eyes.

7. A method for introducing and cultivating Korean pine, according to claim 1, characterized in that: In S3, the grafting strip (1) is made of flexible rubber, and a semi-circular glue injection groove (101) is provided inside. The glue injection groove (101) is used to hold the gel, and an insertion port (102) is provided above the glue injection groove (101). The scion is inserted into the gel through the insertion port (102).

8. A method for introducing and cultivating Korean pine, according to claim 7, characterized in that: The grafting strip (1) surrounds the annular incision of the rootstock and is fixed to the rootstock by a double-sided adhesive strip (2).

9. A method for introducing and cultivating Korean pine, according to claim 1, characterized in that: In S5, when the scion is inserted into the gel, the lower end of the scion has no direct contact with the rootstock incision.

10. A method for introducing and cultivating Korean pine, as described in claim 1, characterized in that: When transplanting in S6, the gel and the scion roots are taken out together for transplantation to avoid damaging the roots.

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