Insecticidal and acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide insect and mite killing, and discloses an insect and mite killing composition and application thereof.The insect and mite killing composition comprises an active ingredient A and an active ingredient B. The active ingredient A is a compound shown in the formula I: # imgabs0 #, and the active ingredient B is any one of bifenazate, chlorfenapyr, flonicamid and flumetnicam. The mass ratio of the active component A to the active component B is (1: 42)-(48: 1). The insecticidal and acaricidal composition disclosed by the invention has a remarkable synergistic effect on acarus and acarus, lepidoptera pests and hemiptera pests, can improve the pesticide use efficiency, reduce the pesticide use amount and reduce the pesticide use cost of farmers, and is beneficial to environment and food safety and environmental protection.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide insecticides and acaricides, and discloses an insecticide and acaricide composition and application thereof. Background Art

[0002] Bifenazate, with the chemical name of 3-(4-methoxybiphenyl-3-yl) isopropyl hydrazine, belongs to the class of hydrazine esters (biphenylhydrazine) acaricides. It was developed by Chemtura Corporation of the United States and entered the U.S. market in 1999 for the control of mites on ornamental plants. Bifenazate has a broad insecticide spectrum and a long lasting effect. It is effective against pest mites such as spider mites, hawthorn spider mites, apple mites and citrus mites. As an inhibitor of complex III of the electron transport chain, it kills pest mites by blocking electron transfer. It has no cross-resistance with existing commercial acaricides and is effective against phytophagous mites, making it suitable for the comprehensive control of pest mites.

[0003] Chlorfenapyr is a pyrrole insecticide and acaricide developed by the American Cyanamid Company. It has the characteristics of broad insecticide spectrum, high efficiency, safety and long lasting effect. The agent acts on the mitochondria in insect cells, destroying the physiological process of converting adenosine diphosphate (ADP) into adenosine triphosphate (ATP) in oxidative phosphorylation. It can effectively prevent and control a variety of pests and mites such as diamondback moth, beet armyworm, armyworm, thrips, and cabbage aphids. It is especially effective against resistant pests and has no cross-resistance with other insecticides.

[0004] Flonicamid, developed by Ishihara Industries Co., Ltd. of Japan, is a pyridine amide insect growth regulator with a unique mechanism of action, especially showing excellent nervous system effects and rapid anti-feeding effects on piercing-sucking mouthparts pests. Flunicamid has contact, stomach poison, systemic and rapid anti-feeding effects. After piercing-sucking mouthparts pests feed on plant sap containing flonicamid, they quickly stop feeding and eventually die of starvation, which can effectively prevent the spread of viral diseases.

[0005] Flumetnicam, CAS number: 158062-71-6, Chinese chemical name: 4-(trifluoromethyl)pyridine-3-carboxamide, is a nicotinamide insecticide. This compound is a metabolite of flubendiamide and has insecticidal activity.

[0006] The compound of formula I belongs to the isoxazoline insecticide, and its CAS number is 2892524-05-7. It belongs to the allosteric modulator of γ-aminobutyric acid (GABA) gated chloride ion channel, has a unique mechanism of action, and has no cross-resistance with other insecticides. The compound has stomach poison, contact killing and certain systemic effects, high rapidity, and egg-killing effect. The insecticidal spectrum covers a variety of pests such as Lepidoptera, Coleoptera, Acarina (eggs, young mites, nymphs, and adult mites), Hemiptera (aphids), and Thysanoptera (thrips).

[0007] In order to deal with the problem of pest resistance, extend the effective use period of pesticides, and ensure the safety of agricultural products, the pesticide combination strategy is particularly important. Scientifically and rationally combining two or more pesticides with different mechanisms of action can reduce the concentration of each pesticide while enhancing the overall control effect and interfering with the development mechanism of pest resistance. This method has been proven in practice to significantly improve control efficiency and reduce pesticide usage, and is a key link in the current integrated pest management strategy. Summary of the invention

[0008] In order to solve the above problems existing in the prior art, the present invention provides an insecticide and acaricide composition. The insecticide and acaricide composition has a significant synergistic effect on Acarina pests, Lepidoptera pests and Hemiptera pests, can improve the efficiency of pesticide use, reduce the amount of pesticide used, reduce the cost of pesticide use for farmers, and is beneficial to the environment, food safety and environmental protection.

[0009] In order to achieve the above object, the present invention adopts the following technical scheme: an insecticide and acaricide composition, the insecticide and acaricide composition comprises active ingredient A and active ingredient B, the active ingredient A is a compound shown in formula I: (Formula I), the active ingredient B is any one of bifenazate, chlorfenapyr, flonicamid, and flumetnicam, and the mass ratio of the active ingredient A to the active ingredient B is 1:42 to 48:1, or any value within the above numerical range.

[0010] Furthermore, the active ingredient B is bifenazate, and the mass ratio of the compound of formula I to bifenazate is 1:24 to 48:1, or any value within the above numerical range;

[0011] The active ingredient B is chlorfenapyr, and the mass ratio of the compound of formula I to chlorfenapyr is 1:25 to 32:1, or any value within the above numerical range;

[0012] The active ingredient B is flonicamid, and the mass ratio of the compound of formula I to flonicamid is 1:20 to 25:1, or any value within the above numerical range;

[0013] The active ingredient B is flumetnicam, and the mass ratio of the compound of formula I to flumetnicam is 1:42 to 28:1, or any value within the above numerical range.

[0014] Furthermore, the active ingredient B is bifenazate, and the mass ratio of the compound of formula I to bifenazate is 1:24 to 32:1, or any value within the above numerical range;

[0015] The active ingredient B is chlorfenapyr, and the mass ratio of the compound of formula I to chlorfenapyr is 1:15 to 26:1, or any value within the above numerical range;

[0016] The active ingredient B is flonicamid, and the mass ratio of the compound of formula I to flonicamid is 1:15 to 20:1, or any value within the above numerical range;

[0017] The active ingredient B is flumetnicam, and the mass ratio of the compound of formula I to flumetnicam is 1:30 to 14:1, or any value within the above numerical range.

[0018] Furthermore, the active ingredient B is bifenazate, and the mass ratio of the compound of formula I to bifenazate is 1:12 to 32:1, or any value within the above numerical range;

[0019] The active ingredient B is chlorfenapyr, and the mass ratio of the compound of formula I to chlorfenapyr is 1:6 to 26:1, or any value within the above numerical range;

[0020] The active ingredient B is flonicamid, and the mass ratio of the compound of formula I to flonicamid is 1:15 to 10:1, or any value within the above numerical range;

[0021] The active ingredient B is flumetnicam, and the mass ratio of the compound of formula I to flumetnicam is 1:24 to 7:1, or any value within the above numerical range.

[0022] Furthermore, the total weight of the composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.01% to 80% of the total weight of the pesticide composition.

[0023] Furthermore, the insecticide and acaricide composition contains, in addition to the active ingredients, auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

[0024] Furthermore, the insecticide and acaricide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid preparation or a liquid preparation.

[0025] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0026] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0027] Furthermore, the solid preparation is a wettable powder or a water-dispersible granule, and the liquid preparation is a suspension, an emulsifiable concentrate, a microemulsion, an aqueous emulsion, or a dispersible oil suspension.

[0028] The invention also discloses the use of the insecticide and acaricide composition for preventing and controlling agricultural, forestry or gardening pests or acarids.

[0029] Furthermore, the harmful mites are harmful mites of the order Acarina, and the harmful insects are harmful insects of the order Lepidoptera or Hemiptera;

[0030] The harmful mites are tea yellow mite, two-spotted spider mite, panonychus mite and cinnabarinus spider mite; the lepidopteran pests are Spodoptera litura, Spodoptera exigua and Plutella xylostella; and the hemiptera pests are aphids.

[0031] In order to obtain the desired insecticidal effect, the dosage of the insecticide and acaricide composition varies depending on various factors, such as the crop to be protected, the type of harmful organisms, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0032] The beneficial effects of the present invention are as follows:

[0033] 1. The insecticide and acaricide composition of the present invention rationally compounds compounds with different action mechanisms, and has significant synergistic effects on different target pests and mites under appropriate proportions;

[0034] 2. The insecticide and acaricide composition of the present invention improves the efficiency of pesticide use, reduces the amount of pesticide used, and reduces the cost of pesticide use;

[0035] 3. The insecticide and acaricide composition of the present invention interferes with the development mechanism of pest resistance, delays the development of pest resistance to a certain extent, and prolongs the service life of the agent.

[0036] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0037] Preparation Example

[0038] Preparation Example 1: 20% Formula I compound·bifenazate suspension (3:1)

[0039] Formula composition: 15% compound of formula I, 5% bifenazate, 3% fatty alcohol polyoxyethylene ether phosphate salt, 1% naphthalene sulfonate formaldehyde condensate, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0040] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0041] Preparation Example 2: 21% Formula I compound·bifenazate water dispersible granules (6:1)

[0042] Formula composition: 18% compound of formula I, 3% bifenazate, 10% sodium salt of polycarboxylate, 5% polyoxyethylene styrene phenol sulfate, 3% BX powder, 6% sodium salt of lignin sulfonate, 10% ammonium sulfate, and kaolin makes up the balance;

[0043] Preparation method: according to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% of water is added, and then kneading, granulation, drying and sieving are carried out to obtain a water-dispersible granule product; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0044] Preparation Example 3: 35% Formula I compound·bifenazate wettable powder (1:6)

[0045] Formula composition: 5% compound of formula I, 30% bifenazate, 10% sodium salt of polycarboxylate, 6% sodium octylphenol polyoxyethylene ether sulfonate, 3% lakai powder BX, and kaolin makes up the balance;

[0046] Preparation method: according to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, stirred evenly in a stirring kettle, and crushed and mixed evenly for multiple times by a jet mill to prepare the wettable powder of the composition of the present invention.

[0047] Preparation Example 4: 2.6% Formula I compound·bifenazate emulsifiable concentrate (1:12)

[0048] Formula composition: 0.2% compound of formula I, 2.4% bifenazate, 10% EO / PO block copolymer, 10% acetophenone, 12% dimethyl sulfoxide, 10% cyclohexanone, 1% calcium dodecylbenzene sulfonate, and xylene makes up the balance;

[0049] Preparation method: according to the formula ratio, the active ingredient, solvent and co-solvent are added into a mixing kettle and stirred to dissolve, then an emulsifier is added, the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in a stirring kettle, and the desired emulsifiable concentrate is obtained after filtering.

[0050] Preparation Example 5: 30% Formula I compound·chlorfenapyr suspension (5:1)

[0051] Formula composition: 25% compound of formula I, 5% fenpyroximate, 3% styrylphenol polyoxyethylene ether phosphate, 5% polyoxyethylene sorbitan monooleate, 2% sodium lignin sulfonate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0052] Preparation method: Same as Preparation Example 1.

[0053] Preparation Example 6: 45% Formula I compound·chlorfenapyr water dispersible granules (1:2)

[0054] Formula composition: 15% compound of formula I, 30% chlorfenapyr, 6% sodium salt of polycarboxylic acid, 5% alkyl naphthalene formaldehyde condensate sulfonate, 10% sodium alkyl polyoxyethylene ether sulfonate, 2% sodium lauryl sulfate, 5% white carbon black, 25% starch, and kaolin makes up the balance;

[0055] Preparation method: Same as Preparation Example 2.

[0056] Preparation Example 7: 11% Formula I compound·chlorfenapyr wettable powder (10:1)

[0057] Formula composition: 10% compound of formula I, 1% chlorfenapyr, 3% tea saponin, 5% succinate sulfonate, 4% lakai powder BX, and kaolin makes up the balance;

[0058] Preparation method: Same as Preparation Example 3.

[0059] Preparation Example 8: 16% Formula I compound·chlorfenapyr EC (1:15)

[0060] Formula composition: 1% compound of formula I, 15% chlorfenapyr, 10% EO / PO block copolymer, 15% acetophenone, 10% N-methylpyrrolidone, 8% sorbitan polyoxyethylene ether, 3% calcium dodecylbenzene sulfonate, and xylene makes up the balance;

[0061] Preparation method: Same as Preparation Example 4.

[0062] Preparation Example 9: 0.7% Formula I compound·chlorfenapyr aqueous emulsion (1:6)

[0063] Formula composition: 0.1% compound of formula I, 0.6% chlorfenapyr, 5% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 3% sodium octylphenol polyoxyethylene ether sulfonate, 20% cyclohexanone, 4% xylene, 0.2% xanthan gum, 5% glycerol, 0.1% sodium sorbate, and deionized water to make up the balance;

[0064] Preparation method: according to the formula ratio, add the active ingredient with the solvent, emulsifier and co-solvent to dissolve into a uniform oil phase; mix water, antifreeze agent and other pesticide adjuvants together to form a uniform water phase; add the oil phase to the water phase while stirring at high speed in the reactor, turn on the shearing machine for high-speed shearing, and shear for about half an hour to form a water-in-oil emulsion.

[0065] Preparation Example 10: 1.9% Formula I compound·chlorfenapyr microemulsion (18:1)

[0066] Formula composition: 1.8% compound of formula I, 0.1% chlorfenapyr, 12% trimethylbenzene, 15% cyclohexanone, 12% Guerbet alcohol polyoxyethylene ether, 5% EO-PO block copolymer, 1% alkylphenol formaldehyde resin polyoxyethylene ether sulfate, 5% propylene glycol, 0.05% organosilicon defoamer, and deionized water to make up the balance.

[0067] Preparation method: according to the formula ratio, the active ingredient solvent, emulsifier, etc. are mixed to obtain the oil phase, the antifreeze solution and water are mixed to obtain the water phase, the oil phase is added to the water phase under stirring and stirred evenly, and the shearing is continued for 10 minutes, and the silicone oil defoaming agent is added and stirred evenly to obtain small droplets of oil phase particles of 0.01 to 0.1 microns, that is, the microemulsion described in the present invention is obtained.

[0068] Preparation Example 11: 22% Formula I compound·flubencarb suspension (1:10)

[0069] Formula composition: 2% compound of formula I, 20% flonicamid, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% alkyl polyoxyethylene ether sodium sulfonate, 5% styrenephenol polyoxyethylene ether phosphate, 3% ethylene glycol oxyethylene polyoxypropylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.1% sodium sorbate, 0.5% silicone oil, and deionized water to make up the balance;

[0070] Preparation method: Same as Preparation Example 1.

[0071] Preparation Example 12: 55% Formula I compound·Fluonicamid water dispersible granules (10:1)

[0072] Formula composition: 50% compound of formula I, 5% flonicamid, 12% dispersant NNO, 4% succinate sulfonate, 3% sodium polycarboxylate, 2% sodium lignin sulfonate, 20% starch, and light calcium carbonate makes up the balance.

[0073] Preparation method: Same as Preparation Example 2.

[0074] Preparation Example 13: 48% Formula I compound·Flunic acid amide wettable powder (1:5)

[0075] Formula composition: 8% compound of formula I, 40% flonicamid, 3% naphthalenesulfonate formaldehyde condensate, 5% dispersant NNO, 7% sodium lignin sulfonate, 5% white sugar, 5% pulverized powder BX, and kaolin makes up the balance;

[0076] Preparation method: Same as Preparation Example 3.

[0077] Preparation Example 14: 21% Formula I compound·Flunic acid amide dispersible oil suspension (5:2)

[0078] Formula composition: 15% compound of formula I, 6% flonicamid, 2% sodium lauryl sulfate, 10% sorbitan oleate polyoxyethylene ether, 3% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 1% silicon dioxide, 1% organic bentonite, 20% 200# solvent oil, and methyl oleate makes up the balance;

[0079] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0080] Preparation Example 15: 21% Formula I compound·flumetnicam suspension (1:6)

[0081] Formula composition: 3% compound of formula I, 18% flumetnicam, 1% sodium dodecyl sulfate, 1% naphthalene sulfonate formaldehyde condensate, 4% tristyrylphenol ethoxylate phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0082] Preparation method: Same as Preparation Example 1.

[0083] Preparation Example 16: 40% Formula I compound·flumetnicam water dispersible granules (7:1)

[0084] Formula composition: 35% compound of formula I, 5% flumetnicam, 12% lignin sulfonate, 5% succinate sulfonate, 3% sodium lauryl sulfate, 5% white carbon black, 25% starch, and kaolin makes up the balance;

[0085] Preparation method: Same as Preparation Example 2.

[0086] Preparation Example 17: 33% Formula I compound·flumetnicam wettable powder (1:2)

[0087] Formula composition: 11% compound of formula I, 22% flonicamid, 4% sodium salt of polycarboxylate, 6% fatty alcohol polyoxyethylene ether sulfate, 7% sodium lignin sulfonate, 8% white sugar, 5% BX powder, and kaolin makes up the balance;

[0088] Preparation method: Same as Preparation Example 3.

[0089] Preparation Example 18: 13% Formula I compound·flumetnicam dispersible oil suspension (1:12)

[0090] Formula composition: 1% compound of formula I, 12% flumetnicam, 2% lignin sulfonate, 12% sorbitan oleate polyoxyethylene ether, 5% alkylphenol polyoxyethylene ether, 3% calcium dodecylbenzene sulfonate, 2% silicon dioxide, 1% organic bentonite, 18% 200# solvent oil, and methyl oleate makes up the balance;

[0091] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0092] Indoor biological activity test

[0093] Example 1: Indoor biological activity test of spider mite

[0094] Test reference: The test refers to NY / T 1154.12-2008 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 12: Spider Mite Slide Dipping Method".

[0095] Test target: Adult two-spotted spider mites;

[0096] Test agents: original drug of the compound of formula I and original drug of bifenazate;

[0097] Preparation of medicine: After dissolving the above raw medicine with a suitable solvent, dilute it with a 0.1% Tween-80 aqueous solution to prepare 5 series of mass concentration gradients.

[0098] Test method: Stick double-sided tape on one end of the slide, and stick the back of the test mite on the double-sided tape, 30 mites per slide, and place it in an environment with a temperature of (26±1)℃ and a RH of 60% to 80% for 4 hours. Use a dissecting microscope to examine, remove dead and inactive individuals, and record the number of live mites. Then, immerse one end of the slide with mites in the corresponding concentration of drug solution, take it out after immersion for 5 seconds, use filter paper to absorb the excess drug solution around the mite body and the surrounding area, and place it under the same breeding conditions for 3 days before inspection. Touch the mite body 5 times with a brush, and the mite is dead if its legs do not move. Use clean water plus the corresponding amount of Tween-80 aqueous solution as a control. When conducting toxicity determination, repeat each concentration 4 times and calculate the lethal concentration.

[0099] Calculation method:

[0100] According to the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0101]

[0102] Where:

[0103] P——mortality rate, in percentage (%);

[0104] K——indicates the number of dead insects, in units of heads;

[0105] N——represents the total number of insects treated, in heads.

[0106]

[0107] Where:

[0108] P1——adjusted mortality rate, in percentage (%);

[0109] P t ——Treatment mortality rate, expressed as percentage (%);

[0110] P0——blank control mortality rate, in percentage (%).

[0111] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0112] Analyze with statistical analysis system to obtain toxicity regression equation, correlation coefficient and LC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0113] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0114]

[0115] Where:

[0116] ATI——Actual Toxicity Index of Mixture;

[0117] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0118] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0119] TTI=TI A ×P A +TI B ×P B

[0120] Where:

[0121] TTI – Theoretical Toxicity Index of Mixtures;

[0122] TI A ——A: toxicity index of the agent;

[0123] P A ——The percentage of agent A in the mixture, in percentage (%);

[0124] TI B ——Toxicity index of agent B;

[0125] P B ——The percentage of agent B in the mixture, in percentage (%).

[0126]

[0127] Where:

[0128] CTC – Co-toxicity coefficient;

[0129] ATI——Actual Toxicity Index of Mixture;

[0130] TTI - Theoretical Toxicity Index of Mixture.

[0131] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0132] The indoor test results are shown in the following table:

[0133] Table 1 Results of indoor biological activity test of compound of formula I and bifenazate against Tetranychus urticae

[0134]

[0135]

[0136] The results of the indoor test showed that the compound of formula I and bifenazate in a suitable mass ratio had a high activity against two-spotted spider mites. The mass ratio of the compound of formula I to bifenazate was 1:24-48:1, and the co-toxicity coefficient against two-spotted spider mites was greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to bifenazate was 1:24-32:1, and the co-toxicity coefficient against two-spotted spider mites was greater than 130, and the synergistic effect was obvious; the mass ratio of the compound of formula I to bifenazate was 1:12-32:1, and the co-toxicity coefficient against two-spotted spider mites was greater than 140, and the synergistic effect was obvious.

[0137] Example 2: Indoor biological activity test on Spodoptera litura

[0138] Test basis: The test is based on NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14: Leaf Dipping Method"

[0139] Test agents: technical drug of the compound of formula I and technical drug of chlorfenapyr;

[0140] Test target: 3rd instar larvae of Spodoptera litura;

[0141] Preparation of medicine: The above raw materials are prepared into mother liquor with organic solvent, and then 5 series of mass concentrations are prepared by equal proportion method with 0.1% Tween-80 aqueous solution.

[0142] Chemical treatment: Select fresh cabbage leaves that have not been treated with chemicals and grow in the same manner. Use a hole puncher to make a suitable leaf disc. Dip the leaf disc into the test solution, take it out and dry it after 10 seconds. Place it in a petri dish containing 1% water agar, and inoculate the test insects. Each treatment is repeated 4 times, and a treatment without chemicals (containing all organic solvents and emulsifiers) is set as a blank control. Each repeat has 20 test insects.

[0143] Feeding and observation: The treated test insects were placed in a temperature of (25±1)℃, a humidity of 60% to 80%, and a photoperiod of L:D=(16:8)h for feeding and observation. The death of the test insects was checked 48h after treatment, and the total number of insects and the number of dead insects were recorded respectively.

[0144] Calculation method:

[0145] According to the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0146]

[0147] Where:

[0148] P——mortality rate, in percentage (%);

[0149] K——indicates the number of dead insects, in units of heads;

[0150] N——represents the total number of insects treated, in heads.

[0151]

[0152] Where:

[0153] P1——adjusted mortality rate, in percentage (%);

[0154] P t ——Treatment mortality rate, expressed as percentage (%);

[0155] P0——blank control mortality rate, in percentage (%).

[0156] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0157] Analyze with statistical analysis system to obtain toxicity regression equation, correlation coefficient and LC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0158] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0159]

[0160] Where:

[0161] ATI——Actual Toxicity Index of Mixture;

[0162] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0163] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0164] TTI=TI A ×P A +TI B ×P B

[0165] Where:

[0166] TTI – Theoretical Toxicity Index of Mixtures;

[0167] TI A ——A: toxicity index of the agent;

[0168] P A ——The percentage of agent A in the mixture, in percentage (%);

[0169] TIB ——Toxicity index of agent B;

[0170] P B ——The percentage of agent B in the mixture, in percentage (%).

[0171]

[0172] Where:

[0173] CTC – Co-toxicity coefficient;

[0174] ATI——Actual Toxicity Index of Mixture;

[0175] TTI - Theoretical Toxicity Index of Mixture.

[0176] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0177] The indoor test results are shown in the following table:

[0178] Table 2 Results of indoor biological activity test on compound of formula I and chlorfenapyr against Spodoptera litura

[0179]

[0180]

[0181] The results of the indoor test showed that the compound of formula I and cypermethrin in a suitable mass ratio showed good activity against Spodoptera litura. The compound ratio of formula I to cypermethrin was 1:25-32:1, and the co-toxicity coefficient against Spodoptera litura was greater than 120, showing a synergistic effect; the mass ratio of formula I to cypermethrin was 1:15-26:1, and the co-toxicity coefficient against Spodoptera litura was greater than 140, showing a significant synergistic effect; the mass ratio of formula I to cypermethrin was 1:6-26:1, and the co-toxicity coefficient was greater than 150, showing a significant synergistic effect.

[0182] Example 3: Indoor bioactivity test of melon aphid

[0183] Test basis: The test refers to the spray method recommended by the Agricultural Industry Standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides" to evaluate the combined action mode.

[0184] Test target: 2nd instar nymphs of melon aphid.

[0185] Test method: Accurately weigh a certain amount of the compound of formula I, flonicamid and flumetnicam technical into a volumetric flask, dissolve them in a suitable solvent, and then prepare 5 concentration gradients in equal proportions using a 0.1% Tween-80 aqueous solution.

[0186] Select 30 aphids of uniform size and place them in a 9cm diameter culture dish. Place it under a Potter spray tower for quantitative spraying. The spray volume is 1mL. After the liquid settles for 1min, take out the test insects and raise them normally in an incubator with a temperature of (26±1)℃, a relative humidity of 60% to 80%, and a photoperiod of 16h:8h (light:darkness). Set 4 replicates for each concentration. Check the number of dead and alive insects in each treatment 24h after the treatment, and calculate the corrected mortality rate of the treatment.

[0187] Calculation method:

[0188] According to the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0189]

[0190] Where:

[0191] P——mortality rate, in percentage (%);

[0192] K——indicates the number of dead insects, in units of heads;

[0193] N——represents the total number of insects treated, in heads.

[0194]

[0195] Where:

[0196] P1——adjusted mortality rate, in percentage (%);

[0197] P t ——Treatment mortality rate, expressed as percentage (%);

[0198] P0——blank control mortality rate, in percentage (%).

[0199] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0200] Analyze with statistical analysis system to obtain toxicity regression equation, correlation coefficient and LC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0201] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0202]

[0203] Where:

[0204] ATI——Actual Toxicity Index of Mixture;

[0205] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0206] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0207] TTI=TI A ×P A +TI B ×P B

[0208] Where:

[0209] TTI – Theoretical Toxicity Index of Mixtures;

[0210] TI A ——A: toxicity index of the agent;

[0211] P A ——The percentage of agent A in the mixture, in percentage (%);

[0212] TI B ——Toxicity index of agent B;

[0213] P B ——The percentage of agent B in the mixture, in percentage (%).

[0214]

[0215] Where:

[0216] CTC – Co-toxicity coefficient;

[0217] ATI——Actual Toxicity Index of Mixture;

[0218] TTI - Theoretical Toxicity Index of Mixture.

[0219] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0220] The indoor test results are shown in the following table:

[0221] Table 3 Results of indoor biological test on melon aphids with compound of formula I and flonicamid

[0222]

[0223] The results of the indoor test showed that the compound of formula I and flonicamid had high activity against melon aphids at an appropriate mass ratio. The mass ratio of the compound of formula I to flonicamid was 1:20-25:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to flonicamid was 1:15-20:1, and the co-toxicity coefficient against melon aphids was greater than 130, with a significant synergistic effect; the mass ratio of the compound of formula I to flonicamid was 1:15-10:1, and the co-toxicity coefficient against melon aphids was greater than 140, with a significant synergistic effect.

[0224] Table 4 Results of indoor biological test on melon aphids with compound of formula I and flumetnicam

[0225]

[0226] The results of the indoor test showed that the compound of formula I and flumetnicam in a suitable mass ratio had high biological activity against melon aphids. The mass ratio of the compound of formula I to flumetnicam was 1:42-28:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. The mass ratio of the compound of formula I to flumetnicam was 1:30-14:1, and the co-toxicity coefficient was greater than 130, with a significant synergistic effect; the mass ratio of the compound of formula I to flumetnicam was 1:24-7:1, and the co-toxicity coefficient against melon aphids was greater than 140, with a significant synergistic effect.

[0227] Field efficacy test

[0228] Example 4: Field test on efficacy of controlling strawberry two-spotted spider mite

[0229] Test location: Strawberry greenhouse in Xinggongshan Village, Dongpu Town, Shaoxing City, Zhejiang Province. The soil in the test plot is loess, with a pH value of 6-7 and an organic matter content of more than 3%, which is suitable for the growth of strawberries.

[0230] Test crop: Strawberry (Akihime).

[0231] Test subject: Two-spotted spider mite.

[0232] Experimental design: The plots were arranged in random blocks, and each plot was determined to have an area of ​​24m 2 , and 4 repeated statistics were set. To prevent the drugs from interfering with each other, protection rows were set between the plots.

[0233] Test method: The test was carried out at the beginning of the strawberry two-spotted spider mite outbreak. The method of application was strictly determined by one staff member using a 3WBD-16B backpack electric sprayer to spray evenly, with a spray volume of 50kg / 667m 2 .

[0234] Survey method: Random sampling was conducted at five points, with 5 leaves fixed at each point, for a total of 25 leaves. The number of mites was checked before application, and the number of live mites was investigated on the 3rd and 10th days after application.

[0235] Calculation method of drug efficacy:

[0236]

[0237] The results of the field efficacy test are shown in the following table:

[0238] Table 5 Results of field trials on efficacy of pesticides against strawberry two-spotted spider mites

[0239]

[0240] 21% Formula I compound·bifenazate water dispersible granules (6:1) and 20% Formula I compound·bifenazate suspension (3:1) showed excellent control effect on strawberry two-spotted spider mite. 3 days after application, the control effect of the compound preparation was 86.54% and 88.69%, which was significantly higher than that of 43% bifenazate suspension and 30% Formula I compound water dispersible granules. 10 days after application, the control effect of 21% Formula I compound·bifenazate water dispersible granules (6:1) and 20% Formula I compound·bifenazate suspension (3:1) continued to increase, reaching 89.07% and 91.81%, respectively. During the test period, no phytotoxicity was observed on strawberry leaves, fruits, etc. at the test dose.

[0241] Example 5: Field efficacy test on control of cabbage armyworm and beet armyworm

[0242] Test location: Cabbage field in Lingbei Village, Gujing Town, Xinhui District, Jiangmen City, Guangdong Province. The land in the test plot is flat and irrigation is convenient, so the water and fertilizer management in each plot is uniform.

[0243] Experimental crops: Cabbage (Jingfeng No. 1).

[0244] Test insects: Spodoptera litura, Spodoptera exigua.

[0245] Experimental design: The experiment set up 5 treatments, including 4 chemical treatments and 1 water control. The experimental plots were arranged in random blocks, with 4 replicates for each treatment and an area of ​​25m 2 .

[0246] Test method: During the test, cabbage was in the rosette stage, and the early larvae of beet armyworm and Spodoptera litura were in full bloom. The Changjiang-10A backpack sprayer was used for spraying at a rate of 750kg / hm 2 The 5-point sampling method was used before and 3 and 7 days after application of pesticides, with 2 plants fixed at each point. The number of live larvae on the leaves of the whole plant was observed, and the control effect of each treatment area was calculated based on the insect population reduction rate.

[0247] Calculation method of drug efficacy:

[0248]

[0249] The results of the field efficacy test are shown in the following table:

[0250] Table 6 Results of field efficacy tests on cabbage armyworm and beet armyworm

[0251]

[0252] As shown in Table 6, 3 days after spraying, 30% Formula I compound·chlorfenapyr suspension (5:1) and 11% Formula I compound·chlorfenapyr wettable powder (10:1) at 45 g / hm 2 The control effects at the doses were 91.49% and 89.98% respectively, while the control agents 30% chlorfenapyr suspension and 30% water dispersible granules of the compound of formula I at 67.5 and 90 g / hm 2 The control effect under the dosage was 71.31% and 69.72%; 7 days after spraying, 30% formula I compound·chlorfenapyr suspension (5:1) and 11% formula I compound·chlorfenapyr wettable powder (10:1) at 45g / hm 2 The control effects of the control agents at 67.5 and 90 g / hm were 94.78% and 92.94%, respectively, while the control agents 30% chlorfenapyr suspension and 30% water dispersible granules of the compound of formula I had a control effect of 94.78% and 92.94% at 67.5 and 90 g / hm 2 The control effect under the dosage is 75.58% and 73.67%

[0253] During the test, the treatment agents did not cause any phytotoxicity to cabbage at the test doses, had little impact on natural enemies of pests, and had a preventive effect on Spodoptera litura, Spodoptera exigua, Pieris rapae, and Plutella xylostella in the test area.

[0254] Example 6: Field test on efficacy of cucumber aphid control

[0255] The test site is a cucumber greenhouse in Xiaohongqiao Village, Shatou Town, Guangling District, Yangzhou City. The soil in the test site is clay soil with medium soil fertility.

[0256] Experimental crops: cucumber (Jinyou 401).

[0257] Experimental design: There are 8 treatments in the experiment, each with 4 replicates, a total of 32 plots, and the plot area is 20m 2 , randomized block arrangement.

[0258] Test method: Spray the cucumbers when they are in the fruiting period and melon aphids are at their peak. Use a Singapore Linong manual sprayer and spray at normal pressure evenly on the front and back of the upper and lower leaves of the cucumbers.

[0259] Survey method: Survey the number of aphids once before spraying, and survey the number of live aphids 1, 3, and 7 days after spraying. Randomly sample 5 points in each plot, survey 2 plants at each point, 5 leaves per plant, and survey the number of aphids on 50 leaves in total in each plot.

[0260] The formula for calculating the protective effect is as follows:

[0261]

[0262] The results of the field efficacy test are shown in the following table:

[0263] Table 7 Results of field efficacy test on cucumber aphids

[0264]

[0265] As shown in Table 7, the compound of formula I combined with flonicamid and flumetnicam has a good control effect on cucumber aphids. The control effect of the combined preparations was 84.22% to 88.38% 1 day after application, and the control effect was 87.51% to 91.44% 3 days after application. The control effects of the two combined preparations were higher than those of the control agents.

[0266] In summary, through indoor toxicity determination and field efficacy test, it can be seen that the composition of the present invention has a good control effect on pests or mites, is safe for target crops, has significant control effect, and is superior to a single agent in delaying the development of resistance and prolonging the lasting effect.

[0267] Although the application describes specific embodiments in detail by means of examples, the disclosure of the application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the application is not limited to the disclosed specific forms. On the contrary, the disclosure of the application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the application, and the scope of the application is limited by the attached claims and their legal equivalents.

Claims

1. An insecticide and acaricide composition, characterized in that: The insecticide and acaricide composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula I: (Formula I), the active ingredient B is any one of bifenazate, chlorfenapyr, flonicamid, and flumetnicam, and the mass ratio of the active ingredient A to the active ingredient B is 1:42 to 48:

1.

2. The insecticide and acaricide composition according to claim 1, characterized in that: The active ingredient B is bifenazate, and the mass ratio of the compound of formula I to bifenazate is 1:24 to 48:1; The active ingredient B is chlorfenapyr, and the mass ratio of the compound of formula I to chlorfenapyr is 1:25 to 32:1; The active ingredient B is flonicamid, and the mass ratio of the compound of formula I to flonicamid is 1:20 to 25:1; The active ingredient B is flumetnicam, and the mass ratio of the compound of formula I to flumetnicam is 1:42 to 28:

1.

3. The insecticide and acaricide composition according to claim 2, characterized in that: The active ingredient B is bifenazate, and the mass ratio of the compound of formula I to bifenazate is 1:24 to 32:1; The active ingredient B is chlorfenapyr, and the mass ratio of the compound of formula I to chlorfenapyr is 1:15 to 26:1; The active ingredient B is flonicamid, and the mass ratio of the compound of formula I to flonicamid is 1:15 to 20:1; The active ingredient B is flumetnicam, and the mass ratio of the compound of formula I to flumetnicam is 1:30 to 14:

1.

4. The insecticide and acaricide composition according to claim 3, characterized in that: The active ingredient B is bifenazate, and the mass ratio of the compound of formula I to bifenazate is 1:12 to 32:1; The active ingredient B is chlorfenapyr, and the mass ratio of the compound of formula I to chlorfenapyr is 1:6 to 26:1; The active ingredient B is flonicamid, and the mass ratio of the compound of formula I to flonicamid is 1:15 to 10:1; The active ingredient B is flumetnicam, and the mass ratio of the compound of formula I to flumetnicam is 1:24 to 7:

1.

5. The insecticide and acaricide composition according to claim 1, characterized in that: The total weight of the composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.01% to 80% of the total weight of the pesticide composition.

6. The insecticide and acaricide composition according to claim 1, characterized in that: The insecticide and acaricide composition contains, in addition to active ingredients, auxiliary ingredients permitted in pesticides, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The insecticide and acaricide composition according to claim 1, characterized in that: The insecticide and acaricide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid formulation or a liquid formulation.

8. The insecticide and acaricide composition according to claim 7, characterized in that: The solid preparation is a wettable powder or a water-dispersible granule, and the liquid preparation is a suspension, an emulsifiable concentrate, a microemulsion, an aqueous emulsion, or a dispersible oil suspension.

9. Use of the insecticidal and acaricidal composition according to any one of claims 1 to 8 for controlling agricultural, forestry or horticultural pests or mites.

10. The use according to claim 9, characterized in that: The harmful mites are of the order Acarina, and the harmful insects are of the order Lepidoptera or Hemiptera; The harmful mites are tea yellow mite, two-spotted spider mite, panonychus mite and cinnabarinus spider mite; the lepidopteran pests are Spodoptera litura, Spodoptera exigua and Plutella xylostella; and the hemiptera pests are aphids.

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