Feed additive for improving daily gain of lambs and application thereof

Abstract

The invention relates to a feed additive for improving daily gain of lambs and application thereof, in particular to a feed additive for improving daily gain of lambs and application thereof.The feed additive comprises bacillus methylotrophicus and honeysuckle vines. The feed additive can remarkably improve the average daily gain and the total antioxidant capacity of the lambs, remarkably change the microbial community structure in excrement and improve the yield of the lambs. The health and growth performance of the lambs are enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of animal husbandry, and in particular to a feed additive for improving the daily weight gain of lambs, and feed containing the feed additive and application thereof. Background Art

[0002] As the reserve force of the sheep farming industry, the growth rate and health of lambs directly affect the economic benefits and sustainable development of the sheep farming industry. A variety of additives have been used to improve the growth performance and health of lambs, but the effect is still limited. In particular, newborn lambs are susceptible to environmental changes due to their immature digestive and immune systems, leading to slow growth and diarrhea.

[0003] Therefore, there is an urgent need to research and develop more effective feed additive compositions to improve the survival rate of lambs, promote their growth and enhance their immunity. Summary of the invention

[0004] Problem that the invention aims to solve

[0005] The invention aims to provide a feed additive which can significantly improve the average daily weight gain and total antioxidant capacity of lambs and significantly change the microbial community structure in feces.

[0006] Bacillus methylotrophicus (BM) is a probiotic isolated from the intestinal tract of the marine organism white bamboo shark. Honeysuckle vine powder has significant antibacterial, antiviral, anti-inflammatory and antioxidant biological activities. The present invention explores the synergistic effect of BM and honeysuckle vine powder in promoting lamb growth and health by compounding and optimizing the content.

[0007] Solutions for solving problems

[0008] The invention provides a feed additive, which comprises methylotrophic bacillus and honeysuckle vine.

[0009] Preferably, the methylotrophic Bacillus is Bacillus methylotrophicus.

[0010] Preferably, the deposit number of the methylotrophic Bacillus is CGMCC NO.13337.

[0011] Preferably, the methylotrophic Bacillus and the honeysuckle vine are both powder particles.

[0012] Preferably, the colony count of the methylotrophic Bacillus is not less than 2×10 9 cfu / g.

[0013] Preferably, the mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:(1-10).

[0014] Preferably, the mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:(5-10), preferably 1:(5-8), and more preferably 1:6.7.

[0015] The present invention provides a method for preparing the feed additive described in any one of the above items, the method comprising the following steps:

[0016] (1) Preparation of probiotic preparation: Methylotrophic Bacillus is prepared into probiotic powder by spray drying;

[0017] (2) Preparation of honeysuckle vine powder: grinding and sieving the honeysuckle vine to obtain honeysuckle vine powder;

[0018] (3) The probiotic powder obtained in step (1) and the honeysuckle vine powder obtained in step (2) are evenly mixed to obtain the feed additive.

[0019] The present invention provides a feed, which comprises any one of the above feed additives or the feed additive prepared by the above method.

[0020] Preferably, the feed additive is added in an amount of 2-5% by weight of the basic feed, preferably 2-3%.

[0021] The present invention provides use of the feed additive according to any one of the above items or the feed additive prepared by the above method in at least one of the following (1) to (4):

[0022] (1) Application in the preparation of products for promoting lamb growth;

[0023] (2) Application in the preparation of products for improving the antioxidant capacity of lambs;

[0024] (3) Application in the preparation of products for enhancing lamb immunity;

[0025] (4) Application in the preparation of products for improving the intestinal microbial community structure of lambs.

[0026] Effects of the Invention

[0027] The present invention provides a feed additive of methylotrophic bacillus and honeysuckle vine powder, and a feed and application thereof. By adding a certain amount of the feed additive of the present invention to lamb feed, the average daily weight gain and total antioxidant capacity of the lamb can be significantly improved, and the microbial community structure in the lamb intestine can be significantly changed, thereby enhancing the health and growth performance of the lamb. The feed additive provided by the present invention not only improves the growth efficiency of the lamb, enhances its antioxidant capacity, but also reduces the diarrhea rate, showing a significant synergistic effect, and has significant application value and promotion prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 Diversity analysis of lamb fecal microbial communities; (A) Chao 1 index compared the α diversity of the four treatment groups CON, BM, VLT, and MIX, showing that the MIX group was significantly different from the other groups; (BC) Simpson index and Shannon index further confirmed the significant differences in α diversity among the groups; (D) OTU Venn diagram. (E) PCoA analysis showed the inter-group differences in β diversity; * indicates p<0.05, ** indicates p<0.01,

[0029] *** indicates p < 0.001, **** indicates p < 0.0001;

[0030] Figure 2 Analysis of the microbial community structure in the CON and MIX groups; (A) shows the differential bacteria at the phylum level; (B) shows the differential bacteria at the genus level; (C) shows the differential bacteria at the species level; (D) The linear discriminant analysis (LDA) score diagram shows the key differential bacteria; (E) The partial phylogenetic tree (Cladogram) reveals the phylogenetic relationship of each microbial category and its changes in different treatment groups;

[0031] Figure 3 Spearman correlation analysis of the MIX group; (A) Correlation between environmental factors and fecal bacteria at the genus level, *P<0.05, **P<0.01. The relevant modules were analyzed using weighted gene co-expression network analysis (WGCNA). The Spearman's rank correlation coefficient was used to generate the relevant network. (B) Heat map of the WGCNA module. (C) Microbiome interaction network and hub microorganisms in the MEblue module and MEyellow. DETAILED DESCRIPTION

[0032] In order to make the technical solutions and beneficial effects of the present invention more clearly understandable, the following is a detailed description by listing specific embodiments. The drawings are not necessarily drawn to scale, and local features may be enlarged or reduced to more clearly show the details of the local features; unless otherwise defined, the technical and scientific terms used herein have the same meanings as those in the technical field to which this application belongs.

[0033] After extensive and in-depth research, the inventors unexpectedly discovered that adding a combination of methylotrophic Bacillus and honeysuckle vine powder to the basic feed of lambs, especially adding them in a certain proportion, the two produce a synergistic effect, which can significantly improve the average daily weight gain and total antioxidant capacity of lambs and significantly change the microbial community structure in the intestines of lambs. Based on this, the technical solution of the present application is proposed.

[0034] The invention provides a feed additive, which comprises methylotrophic bacillus and honeysuckle vine.

[0035] In certain embodiments, the methylotrophic Bacillus is Bacillus methylotrophicus.

[0036] In certain embodiments, the deposit number of the methylotrophic Bacillus is CGMCC NO.13337.

[0037] In certain embodiments, the methylotrophic Bacillus and the honeysuckle vine are both powder particles.

[0038] In some embodiments, the colony count of the methylotrophic Bacillus is not less than 2×10 9 cfu / g.

[0039] In certain embodiments, the mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:(1-10), for example, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10.

[0040] In certain embodiments, the mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:(5-10).

[0041] In certain embodiments, the mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:(5-8).

[0042] In certain embodiments, the mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:6.7.

[0043] In certain embodiments, the methylotrophic Bacillus and the honeysuckle vine powder are in the form of a composition.

[0044] In certain embodiments, the methylotrophic Bacillus and the honeysuckle vine powder are each in the form of separate preparations.

[0045] The present invention provides a method for preparing the feed additive described in any one of the above items, the method comprising the following steps:

[0046] (1) Preparation of probiotic preparation: Methylotrophic Bacillus is prepared into probiotic powder by spray drying;

[0047] (2) Preparation of honeysuckle vine powder: grinding and sieving the honeysuckle vine to obtain honeysuckle vine powder;

[0048] (3) The probiotic powder obtained in step (1) and the honeysuckle vine powder obtained in step (2) are evenly mixed to obtain the feed additive.

[0049] The present invention provides a feed, which comprises any one of the above feed additives or the feed additive prepared by the above method.

[0050] In certain embodiments, the feed additive is added in an amount of 2-5% of the weight of the basic feed, such as 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc.

[0051] In certain embodiments, the feed additive is added in an amount of 2-3% by weight of the basic feed.

[0052] The present invention provides use of the feed additive according to any one of the above items or the feed additive prepared by the above method in at least one of the following (1) to (4):

[0053] (1) Application in the preparation of products for promoting lamb growth;

[0054] (2) Application in the preparation of products for improving the antioxidant capacity of lambs;

[0055] (3) Application in the preparation of products for enhancing lamb immunity;

[0056] (4) Application in the preparation of products for improving the intestinal microbial community structure of lambs.

[0057] In certain embodiments, the feed additive according to any one of the above items or the feed additive prepared by the above method is used in the preparation of a product for improving the daily weight gain of lambs.

[0058] The present invention is further described below by specific examples. Unless otherwise specified herein, "%" represents mass percentage. The materials and reagents in the following examples, unless otherwise specified, are all commonly used materials or reagents in the art, and can be obtained from commercial sources or synthesized by known methods. The experimental methods for which the conditions are not specified in the following implementation cases are usually carried out according to conventional experimental conditions or the conditions recommended by the manufacturers of the relevant reagents (kits).

[0059] Example 1

[0060] Materials preparation:

[0061] Probiotic preparation: Bacillus methylotrophicus (CGMCC No.: 13337) isolated from the intestine of white bamboo shark, prepared into powder by spray drying, with colony forming units greater than 2×10 9 cfu / g.

[0062] Honeysuckle vine powder: Take fresh honeysuckle vines and grind them with a grinder to obtain fine honeysuckle vine powder. Sieve the powder through a sieve to remove larger particles to ensure that the powder is uniform and fine. Store the prepared honeysuckle vine powder in a dry, sealed container.

[0063] Group settings:

[0064] Experimental animals: 32 healthy 7-day-old lactating Nanjiang yellow sheep lambs were selected and randomly divided into 4 groups, with 8 lambs in each group.

[0065] CON group (control group): fed with basic feed (main ingredients include: corn, soybean meal. Nutritional components are as follows: crude protein = 15%, crude fiber = 15%, ash = 10%, calcium = 1%, phosphorus = 0.4%, salt = 1%).

[0066] BM group (experimental group 1): 0.3% of the probiotic preparation was added to the basal diet, calculated based on the basal diet.

[0067] VLT group (experimental group 2): 2% honeysuckle vine powder was added to the basic feed, calculated based on the basic feed.

[0068] MIX group (experimental group 3): 0.3% BM probiotic preparation and 2% honeysuckle vine powder were added to the basal diet at the same time, calculated based on the basal diet.

[0069] Feeding and management:

[0070] Lambs in each group were fed at 7:00 and 19:00 daily, with a feeding amount of 100 g / d for each group, and free access to water. The weight and health status of the lambs were recorded during the feeding period, with a pre-feeding period of 7 days and a formal period of 50 days. The initial weight was recorded, and the weight of all lambs was recorded every 14 days, that is, all lambs were weighed on the last day of the 1st, 3rd, 5th, and 7th weeks. During this period, the health status of the lambs was observed carefully, and illness records, such as diarrhea, were kept. Blood samples were collected from all lambs on the last day of the 7th week. Blood was collected from the posterior jugular vein of each goat between 9:00 and 10:00 in the morning. Serum was separated by centrifugation. MDA, GSH-Px, and T-AOC levels were determined using kits (Nanjing Jiancheng, Nanjing, Jiangsu, China). Feces were collected at the end of the experiment (the last day of the 7th week). The homogenized samples were quickly frozen in liquid nitrogen and stored at -80°C for subsequent analysis.

[0071] Result analysis:

[0072] (1) The feed additive of the present invention significantly increases the daily weight gain of lambs

[0073] In order to explore the effect of the feed additives of the present invention on the growth performance of lambs, the effect of these additives on the weight gain of lambs was first detected. The results are shown in Table 1. Feeding lambs with honeysuckle vine powder can increase the average daily weight gain of lambs, and the effect of adding honeysuckle vine powder and probiotics together is better than adding them alone. There was no difference in the initial body weight of the lambs among the four groups, but there was a trend of difference in the final body weight (p=0.094). Compared with the CON group, feeding probiotic BM alone did not significantly increase the total weight and daily weight gain, but the average daily weight gain of lambs in the VLT group (p=0.007) and the MIX group (p<0.001) was significantly increased. At the same time, it can be observed that the average daily weight gain of lambs in the MIX group is significantly higher than that in the CON group (p=0.014), and the average daily weight gain of the MIX group has an increasing trend compared with the VLT group (p=0.081).

[0074] Table 1

[0075]

[0076] In Table 1, IBW (kg) represents initial body weight, in kilograms; FBW (kg) represents final body weight, in kilograms; TWG (kg / hd) refers to the total body weight gain per head, in kilograms; ADG (g) represents average daily weight gain, in grams. SEM represents standard error, which is used to measure the precision of the sample mean; p-value is used for statistical significance test, and p-value less than 0.05 is considered to be statistically significant, and p-value between 0.05 and 0.1 is considered to be a trend of significant difference. The results of multiple comparisons are presented by letter notation. The differences between the same group with different letters are significant (p<0.05), and the differences between the groups without letters or with the same letters are not significant (p>0.05).

[0077] The above results show that the average daily weight gain of lambs increased when 0.3% probiotic preparations or 2% honeysuckle vine powder were added to the lamb basal diet. It is also worth noting that when probiotic preparations and honeysuckle vine powder were added to the lamb feed at the same time, the weight gain effect was better than adding them alone, indicating that honeysuckle vine powder and probiotic preparations had a synergistic effect in promoting lamb growth. The possible reason is that the chlorogenic acid component in honeysuckle has the effect of increasing intestinal transmembrane electrical resistance (TEER) and reducing horseradish peroxidase (HRP) flux, and has the potential to repair the intestinal barrier. By increasing the intestinal transmembrane electrical resistance, honeysuckle vine powder helps to improve the integrity and function of the intestinal epithelial barrier, while reducing the flux of horseradish peroxidase reduces the penetration of harmful substances through the intestinal barrier, thereby maintaining intestinal health. In addition, BM from marine organisms contains a variety of antimicrobial peptides, which improve the bioavailability of honeysuckle by changing the metabolic function of the intestinal microbiome or directly enhancing the overall function of the intestinal barrier. This synergistic effect further enhances the biological effect of honeysuckle on promoting lamb growth, showing the synergistic mechanism of the two in promoting growth. This joint effect may involve the synergistic metabolism between probiotics and honeysuckle compounds, as well as the co-regulation of the intestinal microenvironment. These mechanisms may not only improve the absorption and utilization efficiency of intestinal nutrients, but also affect the synthesis and release of growth-related hormones, ultimately promoting the growth and development of lambs.

[0078] (2) Effect of the feed additive of the present invention on oxidative stress level in lambs

[0079] In order to further explore the physiological and metabolic mechanism of the feed additive of the present invention on the daily weight gain of lambs, we further explored the changes in the oxidative stress level and metabolism of lambs. The results are shown in Table 2. The results of oxidative stress-related indicators showed that the addition of honeysuckle vine powder or probiotics alone to lamb feed had no effect on the total antioxidant capacity of lambs, but the simultaneous addition of honeysuckle vine powder and probiotics could improve the antioxidant capacity of lambs. The results of T-AOC index showed that there was no significant difference between the BM group and the VLT group and the CON group, but the total antioxidant capacity of the MIX group was significantly higher than that of the CON group (p=0.021), BM group (p=0.022) and VLT group (p=0.012). The simultaneous feeding of honeysuckle vine powder and probiotics to lambs improved the total antioxidant capacity of lambs due to their interaction effect (p=0.07, 0.05<p<0.1 was considered to have an interaction trend).

[0080] Table 2

[0081]

[0082] Table 2 shows the effects of BM and VLT treatment on the antioxidant capacity of lambs. GSH-Px (U / ml) represents glutathione peroxidase activity, in units / ml; MDA (nmol / ml) represents malondialdehyde content, in nanomoles / ml, which is used to measure the level of lipid peroxidation; T-AOC (mmol / L) is the total antioxidant capacity, in millimoles / liter. SEM represents standard error, which is used to measure the precision of the sample mean; p-value is used for statistical significance test, and p-values ​​less than 0.05 are considered to be statistically significant, and p-values ​​between 0.05 and 0.1 are considered to be trends of significant differences. The results of multiple comparisons are presented in letter notation. The differences between the same group with different letters are significant (p<0.05), and the differences between groups without letters or with the same letters are not significant (p>0.05).

[0083] Animal metabolism produces a large number of oxygen free radicals. If they are not removed in time, they can damage cells and tissues and cause diseases. T-AOC, MDA content, and GSH-Px activity can evaluate the body's antioxidant capacity. The results of this experiment showed that the addition of honeysuckle vine powder and probiotic preparations alone had no effect on the antioxidant capacity of lambs, but the simultaneous addition of honeysuckle vine powder and probiotic preparations could significantly improve the total antioxidant capacity of lambs. From the perspective of molecular biology, the combined use of honeysuckle vine powder and probiotic preparations may improve the antioxidant capacity of lambs through multiple mechanisms. First, the flavonoids in honeysuckle vine powder may promote the activation of the promoter of antioxidant response elements (ARE) by regulating the activity of transcription factor Nrf2 (nuclear factor E2-related factor 2), thereby enhancing the expression and activity of intracellular antioxidant enzymes (such as superoxide dismutase, glutathione peroxidase, etc.). Secondly, probiotic preparations may regulate the intestinal mucosal barrier function, reduce the penetration of oxidative stress substances, maintain the stability of the intestinal microenvironment, and thus reduce oxidative damage through the influence of bacterial metabolites. In addition, probiotic preparations may also regulate intestinal immune responses, activate Toll-like receptors and nuclear factor-kB signaling pathways, enhance immune cell activity, and improve the efficacy of intracellular antioxidant defense systems. Finally, honeysuckle vine powder and probiotic preparations may interact with each other to promote the absorption and utilization of antioxidant components in honeysuckle vine powder, further enhancing its antioxidant effect. In summary, the combined use of honeysuckle vine powder and probiotic preparations may improve the total antioxidant capacity of lambs through the synergistic effect of multiple molecular biological mechanisms.

[0084] (3) Effect of the feed additive of the present invention on lamb feces microbiome

[0085] To further study the effects of the combined use of honeysuckle vine powder and probiotics on the gastrointestinal microbial flora of lambs. First, the sufficiency and stability of the amplicon sequencing data of the 16S rRNA gene were evaluated by the rarity curve. The rarity curve gradually stabilized with the increase in sample size, showing that the species in the samples were evenly distributed and the sequencing depth was sufficient to cover the main species in the samples. This result provides a reliable basis for our data and ensures the accuracy of subsequent analysis. Amplicon sequencing of the 16SrRNA gene divided all fecal microorganisms into 6214 OTUs. Venn diagram ( Figure 1 Middle A) shows that the CON group has 552 unique OUTs, the BM group has 384 unique OTUs, the VLT group has 1072 unique OUTs, the MIX group has 1071 unique OUTs, and the four groups have 1173 OTUs in common. The alpha diversity of fecal microorganisms was analyzed by QIIME 2, and the Chao 1, Simpson, and Shannon index results of alpha diversity were ( Figure 1 The PCoA analysis of β diversity further showed that there were significant differences between the MIX group and the CON, BM, and VLT groups ( Figure 1 Middle E).

[0086] Further analysis of the microbial diversity between the groups revealed that the differences in α diversity and β diversity between the BM and VLT groups and the CON group were not significant, indicating that the effects of the BM and VLT treatment conditions alone on the fecal microbial community were small, and the microbial composition was similar to that of the control group. However, the Chao1, Simpson, and Shannon microbial diversity indices of the other groups in the MIX group were significantly different, suggesting that the combined use of honeysuckle vine powder and probiotics can significantly change the composition of fecal microorganisms. Therefore, in order to further explore the effects of the combined effects of honeysuckle vine powder and probiotics on the fecal microbial community of lambs, subsequent studies focused on the comparison between the MIX group and the CON group.

[0087] T-test was used to find out the species with significant differences between MIX and CON groups at each taxonomic level. At the phylum level, compared with the CON group, the MIX group had higher abundances of Verrucomicrobiota and Patescibacteria, and the abundances of Campylobacterota and Desulfobacterota in the MIX group were significantly lower than those in the CON group ( Figure 2At the genus level, the abundances of Akkermansia, Candidatus_Stoquefichus, Faecalibacterium, Candidatus_Saccharimonas, and UBA1819 in the MIX group were significantly higher than those in the CON group, and the abundances of Rikenellaceae_RC9_gut_group, Campylobacter, Turicibacter, Desulfovibrio, NK4A214_group, Clostridium_sense_stricto_1, Ruminococcus, Romboutsia, and Defluviitaleaceae_UCG_011 were significantly lower than those in the CON group ( Figure 2 At the species level, the abundances of Bacteroides_massiliensis, Akkermansia_muciniphila, Alistipes_finegoldii, Parabacteroides_merdae, and Bacteroides_caecigallinarum in the MIX group were significantly higher than those in the CON group, and the abundance of Romboutsia_sp_DR1 was significantly lower than that in the CON group ( Figure 2 Linear discriminant analysis effect size (LEfSe) further determined the abundance differences of bacterial taxa between the MIX and CON groups. When LDA>4, o_Pseudomonadales, f_Moraxellaceae, g_Acinetobacter, g_Bacteroides, f_Bacteroidaceae, and f_Muribaculaceae were enriched in the MIX group, and f_Barnesiellaceae, g_Prevotellaceae_UCG_004, g_Rikenellaceae_RC9_gut_group, and f_Rikenellaceae were enriched in the CON group ( Figure 2 middle DE).

[0088] The above results showed that there were significant differences in ADW and T-AOC between the CON group and the MIX group. Spearman correlation analysis was then performed ( Figure 3A) was used to determine the relationship between fecal bacterial genera and ADW and T-AOC. The results showed that three genera in feces were negatively correlated with ADW, including Prevotellaceae_UCG.004 (p<0.01, R=-0.77), UCG.005 (p<0.01, R=-0.53), and Rikenellaceae_RC9_gut_group (p<0.01, R=-0.58), Alistipes was positively correlated with T-AOC (p<0.01, R=0.76), and Blautia was negatively correlated with T-AOC (p=0.03, R=-0.44).

[0089] At the genus level, the relationship between the indicators and the microbiome was further investigated using WGCNA. A total of six related microbiome modules were identified ( Figure 3 The network derived from the MEblue module showed that o_Bacteroidales_RF16_group, g_Clostridium_sensu_stricto_1, f_Peptostreptococcaceae, and g_Romboutsia were the central microbial groups in the MEblue module (Figure 3C). The network derived from the MEyellow module showed that g_Desulfovibrio, Spirochaetes_bacterium_GWE2_31_10, and Lachnospiraceae_NK4B4_group were the central microbial groups in the MEyellow module (Figure 3C). Figure 3 Middle C).

[0090] Spearman correlation analysis and WGCNA analysis explored the correlation between lamb fecal microbial genera and their growth performance (ADW) and total antioxidant capacity (T-AOC). These analyses revealed complex interactions between microbial community structure and host physiological characteristics, providing new insights into the role of intestinal microorganisms in lamb growth and health. The results of Spearman correlation analysis showed that specific microbial genera such as Prevotellaceae_UCG.004 and Rikenellaceae_RC9_gut_group were significantly negatively correlated with ADW, while Alistipes was significantly positively correlated with T-AOC. These results suggest that the presence of Prevotellaceae_UCG.004 and Rikenellaceae_RC9_gut_group is associated with inefficient energy metabolism, while Alistipes may improve intestinal health and enhance the host's antioxidant capacity through its metabolites such as short-chain fatty acids.

[0091] In addition, WGCNA revealed microbiota modules that were significantly associated with ADW and T-AOC, especially the MEblue and MEyellow modules. The central species in these modules, such as Clostridium_sensu_stricto_1 and Desulfovibrio, may have an important impact on the growth performance and antioxidant status of lambs by regulating the intestinal microenvironment and affecting the host's nutrient absorption and energy metabolism. The activity of Desulfovibrio is related to sulfate reduction, which may affect the redox state of the intestine and thus affect the host's antioxidant capacity. In summary, the present invention provides new insights into the relationship between intestinal microorganisms and growth performance and antioxidant status of lambs, and provides a scientific basis for livestock production optimization strategies based on microbial intervention.

[0092] In summary, the additive provided by the present invention can be used in lamb breeding to promote the growth of lambs, enhance the body's antioxidant capacity, and reduce the diarrhea rate, and the honeysuckle vine powder and the probiotic preparation have a synergistic interaction effect.

[0093] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present invention that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present invention and do not limit the scope of protection of the patent of the present invention.

Claims

1. A feed additive, characterized in that: The feed additive includes methylotrophic bacillus and honeysuckle vine.

2. The feed additive according to claim 1, characterized in that The methylotrophic Bacillus is Bacilus methylotrophicus; Preferably, the deposit number of the methylotrophic Bacillus is CGMCC NO.13337.

3. The feed additive according to claim 1, characterized in that The methylotrophic bacillus and the honeysuckle vine are both powder particles.

4. The feed additive according to claim 1, characterized in that The colony count of the methylotrophic Bacillus is not less than 2×10 9 cfu / g.

5. The feed additive according to any one of claims 1 to 4, characterized in that: The mass ratio of the methylotrophic bacillus to the honeysuckle vine is 1:(1-10).

6. The feed additive according to claim 5, characterized in that The mass ratio of the methylotrophic Bacillus to the honeysuckle vine is 1:(5-10), preferably 1:(5-8), and more preferably 1:6.

7.

7. A method for preparing the feed additive according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: (1) Preparation of probiotic preparation: Methylotrophic Bacillus is prepared into probiotic powder by spray drying; (2) Preparation of honeysuckle vine powder: grinding and sieving the honeysuckle vine to obtain honeysuckle vine powder; (3) The probiotic powder obtained in step (1) and the honeysuckle vine powder obtained in step (2) are evenly mixed to obtain the feed additive.

8. A feed, characterized in that: The feed comprises the feed additive according to any one of claims 1 to 6 or the feed additive prepared by the method according to claim 7.

9. The feed according to claim 8, characterized in that The added amount of the feed additive is 2-5% of the weight of the basic feed, preferably 2-3%.

10. Use of the feed additive according to any one of claims 1 to 6 or the feed additive prepared by the method according to claim 7 in at least one of the following (1) to (4): (1) Application in the preparation of products for promoting lamb growth; (2) Application in the preparation of products for improving the antioxidant capacity of lambs; (3) Application in the preparation of products for enhancing lamb immunity; (4) Application in the preparation of products for improving the intestinal microbial community structure of lambs.

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