Refined molasses and its preparation method and application

Abstract

The present invention provides refined molasses, a preparation method, and applications thereof, relating to the technical field of molasses refining. The molasses preparation method of the present invention comprises the following steps: S1. dissolving sugarcane molasses in water, adding bacterial strains for fermentation, and obtaining fermented molasses; the bacterial strains include Bacillus subtilis and Saccharomyces cerevisiae; S2. adjusting the pH of the fermented molasses to 8-9, allowing it to stand, and then centrifuging, discarding the lower precipitate and retaining the upper liquid; S3. adjusting the pH of the upper liquid to 5.5-7, ultrafiltration, and concentration to obtain a concentrated solution; S4. sterilizing and spray-drying the concentrated solution to obtain refined molasses. The refined molasses prepared by this method has high reducing sugar and crude protein contents and low salt and ash contents.

Description

Technical Field

[0001] The invention belongs to the technical field of molasses refining, in particular to refined molasses and a preparation method and application thereof. Background Art

[0002] Molasses, also known as syrup or treacle, is a viscous, dark brown, semi-fluid substance that contains a large amount of fermentable sugars (primarily sucrose). It is an excellent fermentation raw material and can be used as a substrate or base material for fermentation products such as yeast, monosodium glutamate, and organic acids. It can also be used as an ingredient in certain foods and animal feed. Depending on its source, molasses can be divided into sugarcane molasses, beet molasses, citrus molasses, and corn molasses.

[0003] Molasses is rarely used in livestock production, primarily because its high viscosity makes it difficult to mix into feed, and the polysaccharides it contains require digestion to break down into reducing sugars for absorption, increasing the digestive burden. Furthermore, the crude protein in molasses is primarily non-protein nitrogen, which has a low biological value. Its high mineral content, particularly potassium, magnesium, sodium, and chloride, can cause diarrhea in livestock and poultry. These factors collectively limit its use in livestock production. Summary of the Invention

[0004] The present invention overcomes the above technical problems and provides a refined molasses and a preparation method and application thereof. The refined molasses prepared by the present invention has high contents of reducing sugar and crude protein and low salt content.

[0005] The present invention solves the above technical problems through the following technical solutions.

[0006] A method for preparing refined molasses comprises the following steps:

[0007] S1. Dissolving sugarcane molasses in water, adding bacteria and fermenting to obtain fermented molasses;

[0008] The bacterial species include Bacillus subtilis and Saccharomyces cerevisiae;

[0009] S2. Adjust the pH of the fermented molasses to 8-9, allow to stand, and then centrifuge. Discard the lower precipitate and retain the upper liquid.

[0010] S3. The upper liquid is adjusted to pH 5.5-7, and then ultrafiltered and concentrated to obtain a concentrate;

[0011] S4. The concentrated liquid is sterilized and spray-dried to obtain refined molasses.

[0012] In S1, the sugarcane molasses contains: 24-36 wt% sucrose, 1.5-3 wt% crude protein, 12-18% reducing sugar, and 8-10 wt% salt; wherein the salt contains potassium ions, sodium ions, and magnesium ions, and the reducing sugar includes glucose and fructose.

[0013] In S1, the ash content of the sugarcane molasses is 8.3-9.8%. Increasing the ash content increases the viscosity of the molasses; the higher the ash content in the sugarcane molasses, the higher the viscosity, and when the ash content decreases, the viscosity also decreases.

[0014] In S1, the viscosity of the sugarcane molasses is 6 to 20 Pa·s.

[0015] In S1, the activity of Bacillus subtilis in the strain is >5×10 7 cfu / mL, the optimal value is 5.2×10 7 ~7.3×10 7 cfu / mL.

[0016] In S1, the activity of Saccharomyces cerevisiae in the strain is greater than 5×10 9 cfu / mL, the optimal value is 5.8×10 9 ~6.2×10 9 cfu / mL.

[0017] In S1, the temperature of the water is 28-37°C.

[0018] In S1, the mass ratio of the sugarcane molasses to the bacterial strain is 100:8-10, preferably 100:9-10.

[0019] In S1, the mass ratio of the sugarcane molasses to water is 1:4-10, preferably 1:5-8.

[0020] In S1, the initial pH of the fermentation is controlled at 5.8-6.8, preferably 5.8-6.5.

[0021] In S1, the fermentation temperature is 25-37°C, preferably 28-35°C.

[0022] In S1, the fermentation time is 18 to 24 hours, preferably 20 to 22 hours.

[0023] In S1, the fermentation also requires the introduction of air from the bottom of the fermentation tank, and the flow rate of the fermentation air is 5 to 20 L / min, preferably 7 to 13 L / min.

[0024] In S2, the standing time is 3 to 12 hours.

[0025] In S2, the centrifugal rotation speed is 2000-2800 rpm.

[0026] In S2, the centrifugation time is 3 to 15 minutes.

[0027] In S3, the molecular weight cut-off of the ultrafiltration is 30-100 kDa, preferably 30-50 kDa.

[0028] In S3, the water content after the concentration is 40-60%.

[0029] In S4, the spray drying inlet temperature is 140-180°C, and the outlet temperature is 80-90°C.

[0030] In S4, the refined molasses contains: 60-75 wt% reducing sugar, 4.8-6.7 wt% crude protein, 0.5-1.5 wt% salt; preferably, it contains 62-74% reducing sugar, 5.2-6.7% crude protein, and 0.5-1.38 wt% salt.

[0031] In S4, the ash content of the refined molasses is 1.76-2.08%.

[0032] In S4, the shelf life of the refined molasses is 6 to 9 months. The refining process of the present invention includes drying, granulation and sterilization operations, which is more conducive to the preservation of the refined molasses.

[0033] In S4, the refined molasses is solid, preferably in the form of blocks, granules or powder.

[0034] The application of the refined molasses as mentioned above is in chicken feed, ruminant feed and pig feed;

[0035] Among them, the chicken feed includes breeding-type laying hen feed and fattening-type broiler feed; the ruminant feed includes cattle feed and sheep feed.

[0036] Furthermore, the refined molasses is added to replace 2-5% of chicken feed;

[0037] Furthermore, the refined molasses is added to replace 3-8% of ruminant feed;

[0038] Furthermore, the refined molasses is added to replace 3-8% of pig feed;

[0039] Furthermore, the refined molasses is dissolved in water and directly provided to chickens, ruminants or pigs for drinking, wherein the added amount of the refined molasses is 2-5% of the mass of water.

[0040] On the basis of conforming to the common sense in this field, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present invention.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] 1. The present invention uses bacterial fermentation to degrade polysaccharides into reducing monosaccharides that are easily digested and absorbed by animals. This fermentation process not only promotes nutrient absorption in animals but also effectively removes salt from sugarcane molasses by consuming excess salt. This also prevents diarrhea in animals caused by excessive sodium, magnesium, and potassium ions in sugarcane molasses.

[0043] 2. After fermentation and refining, the content of polysaccharides, metal ions and colloidal substances in molasses is significantly reduced. These components are the main reason for the viscosity of molasses. At the same time, this process can also effectively remove the non-protein nitrogen content in crude protein. In some preferred embodiments, the refined molasses contains 60-75wt% reducing sugars, 4.8-6.7wt% crude protein, 0.5-1.5wt% salt, and 1.76-2.08wt% ash; in some preferred embodiments, the refined molasses contains 62-74wt% reducing sugars, 5.2-6.7wt% crude protein, and 0.5-1.38wt% salt; adjusting the proportions of these components helps to improve the nutritional value and application effect of molasses.

[0044] 3. The refined molasses fermentation method of the present invention converts viscous sugarcane molasses, which is characterized by high water content, polysaccharide, salt, and ash content, into a solid granular product. This conversion significantly simplifies the transportation and storage of molasses and effectively addresses the potential deterioration of molasses during these processes. By reducing the water content and lowering the polysaccharide, salt, and ash content, the physical state of the molasses is improved, thereby enhancing its stability during storage and transportation.

[0045] 4. Due to its sweet aroma and strong appetizing properties, the refined molasses of the present invention can be added to animal diets as a substitute for feed additives. Furthermore, feeding refined molasses to animals with intestinal dysplasia or those who have not yet fully recovered from illness can provide significant health benefits. By providing easily digestible and absorbable nutrients, refined molasses contributes to intestinal health and recovery in these animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 Thin layer chromatograms of cane molasses and refined molasses. DETAILED DESCRIPTION

[0047] To facilitate understanding of the present invention, the present invention will be described more comprehensively and meticulously below in conjunction with preferred embodiments, but the protection scope of the present invention is not limited to the following specific embodiments.

[0048] Unless otherwise defined, all technical terms used hereinafter have the same meanings as those generally understood by those skilled in the art. The technical terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0049] The "ranges" disclosed herein are defined in terms of lower and upper limits. A given range is defined by selecting a lower limit and an upper limit, and the selected lower and upper limits define the boundaries of the particular range. Ranges defined in this manner can be inclusive or exclusive of the end values ​​and can be combined in any manner, i.e., any lower limit can be combined with any upper limit to form a range. For example, if ranges of 60-120 and 80-110 are listed for a particular parameter, it is understood that ranges of 60-110 and 80-120 are also contemplated. Furthermore, if the minimum range values ​​listed are 1 and 2, and if the maximum range values ​​listed are 3, 4, and 5, then the following ranges are all contemplated: 1-3, 1-4, 1-5, 2-3, 2-4, and 2-5. In this disclosure, unless otherwise indicated, the numerical range "ab" is an abbreviation for any combination of real numbers between a and b, where a and b are both real numbers. For example, the numerical range "0-5" indicates that all real numbers between "0-5" have been listed herein, and "0-5" is merely an abbreviation for these numerical combinations. In addition, when a parameter is expressed as an integer ≥ 2, it is equivalent to disclosing that the parameter is, for example, an integer of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0050] Unless otherwise specified, all embodiments and optional embodiments of the present invention can be combined with each other to form new technical solutions.

[0051] Unless otherwise specified, all technical features and optional technical features of the present invention can be combined with each other to form a new technical solution.

[0052] Unless otherwise specified, all steps of the present invention may be performed sequentially or randomly, preferably sequentially. For example, "the method includes steps (a) and (b)" indicates that the method may include steps (a) and (b) performed sequentially, or may include steps (b) and (a) performed sequentially. For example, "the method may further include step (c)" indicates that step (c) may be added to the method in any order, for example, the method may include steps (a), (b) and (c), or may include steps (a), (c) and (b), or may include steps (c), (a) and (b), etc.

[0053] Unless otherwise specified, the terms "include" and "comprising" used in the present invention may be open-ended or closed-ended. For example, "include" and "comprising" may mean that other components not listed may also be included or that only the listed components are included.

[0054] Unless otherwise specified, the term "or" is inclusive in this disclosure. For example, the phrase "A or B" means "A, B, or both A and B." More specifically, the condition "A or B" is satisfied by any of the following conditions: A is true (or exists) and B is false (or does not exist); A is false (or does not exist) and B is true (or exists); or both A and B are true (or exist).

[0055] The sugarcane molasses used in the following examples or comparative examples contained 33% sucrose, 30% water, 1.8% crude protein, 14% reducing sugar, and 9% salt; Bacillus subtilis was purchased from Leshengyuan Biotechnology (Nanjing) Co., Ltd., and Saccharomyces cerevisiae was purchased from Tianjin Yunlizhixing Biotechnology Co., Ltd.

[0056] Example 1

[0057] The preparation method of the refined molasses of the present embodiment is as follows:

[0058] S1. 50 kg of sugarcane molasses was dissolved in 35 ° C water, mixed and sterilized, and then the bacteria were added. The initial pH of the fermentation was 6.4, the fermentation temperature was controlled at 28 to 30 ° C, and the fermentation also required air to be introduced from the bottom of the fermenter at an air flow rate of 7 L / min. After stirring and fermenting for 20 h, the fermented molasses was obtained;

[0059] In this example, the bacterial strain was prepared at a 0.5 wt% bacterial solution by mixing LB medium powder (purchased from Beijing Yita Biotechnology Co., Ltd.) and water in a mass ratio of 10:1, sterilizing the mixture, and then fermenting and inoculating the mixture at 30°C with stirring (200 rpm) for 24 hours to obtain the bacterial strain. The activity of the bacterial strain can be controlled by adjusting the fermentation inoculation time, which can range from 12 to 48 hours.

[0060] In this example, the activity of Bacillus subtilis in the strain was 5.2×10 7 cfu / mL, and the activity of Saccharomyces cerevisiae was 6.2×10 9 cfu / mL;

[0061] In this embodiment, the mass ratio of sugarcane molasses to bacterial strain is 100:9; the mass ratio of sugarcane molasses to water is 1:8.

[0062] S2. The pH of the fermented molasses was adjusted to 9, allowed to stand for 4 h, and centrifuged at 2500 rpm for 10 min. The lower precipitate was discarded and the upper liquid was retained;

[0063] S3. The supernatant was adjusted to pH 6.0, and then ultrafiltration with a molecular weight cutoff of 30 kDa was performed and concentrated to a water content of 43%;

[0064] S4. The concentrate is sterilized and spray-dried with the air inlet temperature at 150°C and the air outlet temperature at 80°C to obtain refined molasses. The thin layer chromatograms of refined molasses and cane molasses are shown in Figure 2. Figure 1 ,from Figure 1 It can be seen that sugarcane molasses has a high sucrose content and a low reducing sugar content, while refined molasses has a high reducing sugar content and does not contain sucrose; in addition, the gray color at the bottom of the sugarcane molasses thin layer chromatography plate is salt, in comparison, refined molasses has a low salt content.

[0065] Example 2

[0066] In this example, the activity of Bacillus subtilis in the strain was 6.2×10 7 cfu / mL, Saccharomyces cerevisiae activity 6.2×10 9 cfu / mL, and other steps and parameters were the same as in Example 1.

[0067] Example 3

[0068] In this embodiment, the mass ratio of sugarcane molasses to bacterial strain is 100:10, and the other steps and parameters are the same as those in Example 1.

[0069] Example 4

[0070] In this example, the initial pH of the fermentation was 5.8, and the other steps and parameters were the same as those in Example 1.

[0071] Example 5

[0072] In this example, the initial pH of the fermentation was 6.0, and the other steps and parameters were the same as those in Example 1.

[0073] Example 6

[0074] In this example, the initial pH of the fermentation was 6.2, and the other steps and parameters were the same as those in Example 1.

[0075] Example 7

[0076] In this example, the fermentation temperature was controlled at 32-35° C., and the other steps and parameters were the same as those in Example 1.

[0077] Example 8

[0078] In this example, the fermentation time was 18 h, and the other steps and parameters were the same as those in Example 1.

[0079] Example 9

[0080] In this example, the fermentation time was 22 h, and the other steps and parameters were the same as those in Example 1.

[0081] Example 10

[0082] In this example, the fermentation time was 24 h, and the other steps and parameters were the same as those in Example 1.

[0083] Example 11

[0084] In this embodiment, the air flow rate is 8 L / min, and the other steps and parameters are the same as those in Example 1.

[0085] Example 12

[0086] In this embodiment, the air flow rate is 9 L / min, and the other steps and parameters are the same as those in Example 1.

[0087] Example 13

[0088] In this example, 50 kg of sugarcane molasses was dissolved in 28°C water;

[0089] In this example, the initial pH of the fermentation was 5.2;

[0090] In this embodiment, the fermentation time is 22h;

[0091] In this embodiment, the air flow rate is 8L / min;

[0092] Other steps and parameters are the same as in Example 1.

[0093] Comparative Example 1

[0094] In this comparative example, the activity of Bacillus subtilis in the strain was 9.2×10 7 cfu / mL, Saccharomyces cerevisiae activity 8.0×10 9 cfu / mL, and other steps and parameters were the same as in Example 1.

[0095] Comparative Example 2

[0096] In this comparative example, the mass ratio of sugarcane molasses to bacterial strain is 100:7, and the other steps and parameters are the same as those in Example 1.

[0097] Comparative Example 3

[0098] In this comparative example, the mass ratio of sugarcane molasses to bacterial strain is 100:11, and the other steps and parameters are the same as those in Example 1.

[0099] Comparative Example 4

[0100] In this comparative example, the initial pH of the fermentation was 5.5, and the other steps and parameters were the same as those in Example 1.

[0101] Comparative Example 5

[0102] In this comparative example, the fermentation temperature was controlled at 23-24° C., and the other steps and parameters were the same as those in Example 1.

[0103] Comparative Example 6

[0104] In this example, the fermentation time was 26 h, and the other steps and parameters were the same as those in Example 1.

[0105] Comparative Example 7

[0106] In this comparative example, the molecular weight cut-off was 10 kDa, and the other steps and parameters were the same as those in Example 1.

[0107] Test Example 1

[0108] The results of the component tests of the above examples and comparative examples are shown in Table 1.

[0109] Table 1

[0110] - Reducing sugar / % Crude protein / % Salt / % Ash / % Example 1 67 6.2 0.62 1.95 Example 2 61 6.1 0.83 1.82 Example 3 69 6.3 0.58 1.78 Example 4 62 6.2 1.28 1.85 Example 5 74 6.7 0.89 1.79 Example 6 60 6.4 1.38 1.93 Example 7 63 5.4 0.96 1.90 Example 8 68 5.2 0.51 1.81 Example 9 73 5.6 0.35 1.77 Example 10 70 4.9 0.63 1.99 Example 11 71 5.1 0.82 1.85 Example 12 66 5.3 1.33 2.06 Example 13 69 6.5 0.79 2.03 Comparative Example 1 52 6.5 1.78 2.56 Comparative Example 2 45 4.8 1.64 5.72 Comparative Example 3 48 4.3 0.94 2.11 Comparative Example 4 58 5.1 1.66 2.47 Comparative Example 5 50 4.6 2.35 3.22 Comparative Example 6 54 4.2 0.49 2.67 Comparative Example 7 53 3.6 0.56 1.34

[0111] Crude protein test method: GB / T 6432.

[0112] Salt content test method: GB / T 13885.

[0113] Ash content test method: GB / T 6438.

[0114] The test method for reducing sugar (measured in glucose) is as follows:

[0115] (1) 1 mg / ml glucose standard solution: Accurately weigh 100 mg of dry constant weight glucose, dissolve it in a small amount of distilled water, and then dilute to 100 ml with distilled water, i.e., the glucose content is 1.0 mg / ml.

[0116] (2) 3,5-Dinitrosalicylic acid reagent: Weigh 6.3 g of 3,5-dinitrosalicylic acid and 262 ml of 2 mol / L NaOH and add them to a hot solution of potassium sodium tartrate (182 g of potassium sodium tartrate dissolved in 500 ml of water). Then add 5 g of crystalline phenol and 5 g of sodium bisulfite and dissolve them in it. Stir to dissolve. After cooling, adjust the volume to 1000 ml and store in a brown bottle.

[0117] (3) Equipment: spectrophotometer, balance, water bath, electric furnace, test tubes, etc.

[0118] (4) Draw a glucose standard curve

[0119] Prepare eight 15mm x 180mm test tubes and add 1.0mg / ml glucose standard solution and distilled water according to Table 2. Mix the solutions thoroughly and heat in boiling water for 5 minutes. Immediately cool to room temperature with cold water. Add 21.5ml of distilled water to each tube and shake well. Measure the absorbance at λ = 540nm and plot a standard curve with the glucose content (mg) as the abscissa and the absorbance as the ordinate.

[0120] Table 2

[0121]

[0122] (5) Sample preparation

[0123] Accurately weigh 0.5 g of refined molasses obtained in the above Examples or Comparative Examples and place it in a 100 ml beaker. First, add a small amount of distilled water (about 2 ml) to make a paste. Then add 40 ml of distilled water and mix thoroughly. Incubate in a 50°C constant temperature water bath for 20 minutes, stirring occasionally to allow the reducing sugars to leach out. Filter and collect the filtrate in a 50 ml volumetric flask. Add distilled water to the mark to prepare the sample.

[0124] (6) Determination of reducing sugar in samples

[0125] Mix all the solutions in each tube evenly, heat in boiling water for 5 minutes, remove from the heat, and immediately cool to room temperature with cold water. Add 21.5 ml of distilled water to each tube and shake well. Measure the absorbance at λ = 540 nm. After determining the reducing sugar content in the sample solution, use the sample's absorbance value to find the corresponding reducing sugar content on the standard curve. Calculate the percentage of reducing sugar and total sugar in the sample using the following formula: Reducing sugar (as glucose) % = C × V / m × 1000C;

[0126] Where: concentration of reducing sugar extract, mg / ml; V - total volume of reducing sugar extract, ml; m - sample weight, g; 1000 - coefficient for converting mg to g.

[0127] Application Examples

[0128] In an applied experiment conducted on a large-scale beef cattle farm, 60 cattle weighing 382.7 ± 5.68 kg were selected. The experimental design employed a 10 × 3 block design, with three replicates in each experimental and control groups, each containing 10 cattle. The control group was fed a basal diet alone, while the experimental group received a basal diet supplemented with 5% refined molasses (prepared according to Example 13) in place of corn. The specific composition of the basal concentrate is shown in Table 3.

[0129] Table 3

[0130]

[0131]

[0132] The pre-feeding period was 15 days and the experimental period was 45 days. Daily feed intake data for each beef cattle was recorded. After the experiment, the average daily feed intake of the experimental and control groups was calculated. Specific data are shown in Table 4.

[0133] Table 4

[0134] - Experimental group control group Feed intake / (kg / day) 11.3 14.6

[0135] In livestock and poultry farming, the use of refined molasses can also promote intestinal development, improve intestinal health, and increase feed digestibility. This not only helps the growth and development of animals, but also improves overall feeding efficiency.

[0136] Furthermore, using refined molasses as a feed substitute offers several benefits: improving the condition of animal feces and reducing loose stools; reducing fecal volume and odor in the barn, thereby improving the breeding environment. Refined molasses has a significant positive effect on the recovery of animals with intestinal dysplasia or those who have not fully recovered from illness. Furthermore, refined molasses can increase the survival rate of small animals, providing better economic benefits for breeders.

[0137] Unless otherwise specified, all raw materials, reagents, instruments, and equipment used in the present invention can be purchased from the market or prepared by existing methods. The specific embodiments described above further illustrate the purpose, technical solutions, and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims

1. A method for preparing refined molasses, characterized in that: The following steps are involved: S1. Dissolving sugarcane molasses in water, adding bacteria and fermenting to obtain fermented molasses; The bacterial strains include Bacillus subtilis and Saccharomyces cerevisiae; the mass ratio of the sugarcane molasses to the bacterial strains is 100:8-10; The activity of Bacillus subtilis in the strain is 5.2×10 7 ~7.3×10 7 cfu / mL; the activity of Saccharomyces cerevisiae in the strain was 5.8×10 9 ~6.2×10 9 cfu / mL; The initial pH of the fermentation is controlled at 5.8-6.8; the fermentation temperature is 28-35°C, the fermentation time is 18-24 hours; the fermentation air flow rate is 5-20 L / min; S2. Adjust the pH of the fermented molasses to 8-9, allow to stand, and then centrifuge. Discard the lower precipitate and retain the upper liquid. S3. The upper liquid was adjusted to pH 5.5-7, and then ultrafiltered and concentrated to obtain a concentrate; the ultrafiltration molecular weight cutoff was 30-100 kDa; S4. The concentrated liquid is sterilized and spray-dried to obtain refined molasses.

2. The method for preparing refined molasses according to claim 1, wherein The sugarcane molasses comprises: 24-36 wt% sucrose, 1.5-3 wt% crude protein, 12-18% reducing sugar, and 8-10 wt% salt; wherein the salt comprises potassium ions, sodium ions and magnesium ions, and the reducing sugar comprises glucose and fructose; And / or, the ash content of the sugarcane molasses is 8.3-9.8%.

3. The method for preparing refined molasses according to claim 1, wherein The mass ratio of the sugarcane molasses to the bacterial strain is 100:9-10; And / or, the mass ratio of the sugarcane molasses to water is 1:4-10.

4. The method for preparing refined molasses according to claim 1, wherein The water content after the concentration is 40-60%.

5. The method for preparing refined molasses according to claim 1, wherein The spray drying inlet temperature is 140-180°C, and the outlet temperature is 80-90°C.

6. The refined molasses prepared by the method for preparing the refined molasses according to any one of claims 1 to 5, wherein: The refined molasses contains: 60-75% reducing sugar, 4.8-6.7 wt% crude protein, and 0.5-1.5 wt% salt; And / or, the ash content of the refined molasses is 1.76-2.08%.

7. The use of the refined molasses in preparing feed according to claim 6, characterized in that: The application is in chicken feed, ruminant feed and pig feed; Wherein, the chicken feed includes breeding type layer feed and fattening type broiler feed; The ruminant feed includes cattle feed and sheep feed.

8. The use of refined molasses according to claim 7, characterized in that Satisfy at least one of the following conditions a to d: a. The refined molasses is added as an alternative to 2 to 5% chicken feed; b. The refined molasses is added as a substitute for 3 to 8% of ruminant feed; c. The refined molasses is added as a substitute for 3 to 8% of pig feed; d. The refined molasses is dissolved in water and directly provided to chickens, ruminants or pigs for drinking, wherein the amount of the refined molasses added is 2 to 5% of the mass of the water.

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