Aquatic fermented feed auxiliary material as well as preparation method and application thereof

Abstract

The invention provides an aquatic product fermented feed auxiliary material as well as a preparation method and application thereof. The aquatic product fermented feed auxiliary material comprises a fermentation raw material and a fermentation strain composition, and the mass of the fermentation strain composition is 0.3%-0.5% of the mass of the fermentation raw material; wherein the fermentation raw materials comprise the following components in parts by weight: 10-25 parts of peanut meal, 20-45 parts of soybean meal, 25-35 parts of bran and 5-15 parts of yeast metabolite; the fermentation strain composition is prepared from 45 to 55 parts of saccharomyces cerevisiae boulardii, 10 to 15 parts of bacillus subtilis, 2 to 4 parts of lactobacillus plantarum, 14 to 18 parts of alkaline protease, 15 to 19 parts of glucose and 36 to 48 parts of water. In the application process, the aquatic product fermented feed auxiliary material can improve the nutritional value of the aquatic product fermented feed auxiliary material, can promote food calling of aquatic animals, has a good growth promoting effect on the aquatic animals, and can also improve the water quality of the growth environment of the aquatic animals.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation, and in particular to an aquatic fermented feed auxiliary material, a preparation method and application thereof. Background Art

[0002] In recent years, with the rapid development of my country's aquaculture industry, the demand for feed has gradually increased, and the quality requirements have become higher and higher. Under this condition, the research and development of safe, efficient, healthy and environmentally friendly microbial feed and fermented feed is a major hotspot in the development of the aquaculture industry.

[0003] Microbial fermented feed refers to the process of fermenting roughage that is not easily absorbed and utilized by animals into feed that is easier to digest and absorb through the metabolic activities of microorganisms themselves. At present, aquatic fermented feed powders are available in dry and wet forms, and wet feed is more conducive to the preservation of live bacteria. The factors affecting the quality of fermented feed mainly include fermentation raw materials, fermentation strains and fermentation parameters. It can be seen from the existing technology that a variety of mixed feed raw materials have become the mainstream of fermented feed substrate selection, but the focus is mainly on the impact of fermentation on feed raw materials, while less attention is paid to functional raw materials.

[0004] Yeast culture was applied to aquaculture in the 1980s. Through a series of targeted application tests and production applications, it was found that as a fermentation product, aquatic yeast culture plays a positive role in maintaining the survival ability of fish and shrimp and promoting the growth of fish and shrimp. Therefore, how to use yeast-related substances to provide a feed that promotes the growth of aquatic fermented feed additives has great application significance. Summary of the invention

[0005] The main purpose of the present invention is to provide an aquatic fermented feed auxiliary material, a preparation method and application thereof, so as to solve the problem that the aquatic fermented feed in the prior art has a low promoting effect on the growth of aquatic animals.

[0006] In order to achieve the above-mentioned purpose, according to the first aspect of the present invention, there is provided an aquatic fermented feed auxiliary material, comprising: a fermentation raw material and a fermentation strain composition, the mass of the fermentation strain composition being 0.3%-0.5% of the mass of the fermentation raw material; wherein, in parts by weight, the fermentation raw material comprises: 10-25 parts of peanut meal, 20-45 parts of soybean meal, 25-35 parts of bran and 5-15 parts of yeast metabolites; the fermentation strain composition comprises: 45-55 parts of Saccharomyces boulardii, 10-15 parts of Bacillus subtilis, 2-4 parts of Lactobacillus plantarum, 14-18 parts of alkaline protease, 15-19 parts of glucose and 36-48 parts of water.

[0007] Furthermore, the viable counts of the microorganisms in the fermented bacteria composition are: Saccharomyces boulardii ≥ 10 billion CFU / g, Bacillus subtilis ≥ 2 billion CFU / g, and Lactobacillus plantarum ≥ 2.5 billion CFU / g.

[0008] Furthermore, the enzymatic activity of the alkaline protease is ≥30000 U / g.

[0009] Furthermore, the diameter sizes of the peanut meal and the soybean meal are independently 18-24 meshes.

[0010] In order to achieve the above-mentioned purpose, according to the second aspect of the present invention, a method for preparing the above-mentioned aquatic fermented feed auxiliary material is provided, the method comprising: S1, mixing Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water, and performing activation culture to obtain an activated culture solution; S2, mixing the activated culture solution with peanut meal, soybean meal, bran and yeast metabolites, and performing anaerobic fermentation culture to obtain the aquatic fermented feed auxiliary material.

[0011] Furthermore, the temperature of the activation culture is 30°C-40°C; preferably, the time of the activation culture is 20-30 minutes.

[0012] Furthermore, the temperature of the anaerobic fermentation culture is 25°C-35°C; preferably, the time of the anaerobic fermentation culture is 72-96h.

[0013] Furthermore, the aquatic fermented feed auxiliary materials include wet materials and dry materials, wherein the aquatic fermented feed auxiliary materials obtained in S2 are wet materials, and the wet materials are dried to obtain dry materials; preferably, the drying temperature is 50-55°C.

[0014] In order to achieve the above object, according to the third aspect of the present invention, there is provided an application of the above-mentioned fermented aquatic feed auxiliary material or the fermented aquatic feed auxiliary material prepared by the above-mentioned method in aquaculture.

[0015] Furthermore, the amount of auxiliary materials added to the aquatic fermented feed is 100-200 kg of wet material and 100-200 kg of dry material added independently to each ton of commercial feed.

[0016] By applying the technical scheme of the present invention, the aquatic fermented feed auxiliary material of the present application includes yeast metabolites and Saccharomyces boulardii. The product obtained by the composition ratio of the feed auxiliary material of the present application improves the nutritional value of the aquatic fermented feed auxiliary material during the application process, can promote the feeding of aquatic animals, has a good growth-promoting effect on aquatic animals, can regulate the balance of intestinal flora of aquatic animals, enhance their anti-stress and antioxidant capabilities, and can also improve the water quality of the growth environment of aquatic animals. DETAILED DESCRIPTION

[0017] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0018] Glossary:

[0019] Yeast metabolites: The fermentation metabolites of yeast cultured with molasses, which are refined through concentration, drying or non-drying processes to produce yeast metabolites products rich in amino acids, organic acids, fulvic acid, polysaccharides and other substances as well as major, medium and trace elements.

[0020] Saccharomyces boulardii: a single-cell fungus belonging to the genus Saccharomyces, a strain of the subspecies of Saccharomyces cerevisiae, having nutritional, antibacterial, antitoxin, anti-inflammatory and intestinal improvement effects. The Saccharomyces boulardii used in the present application is selected from existing published strains.

[0021] Yeast culture: refers to the microecological product formed by yeast on a specific culture medium after sufficient anaerobic fermentation under specific process conditions. It is mainly composed of yeast extracellular metabolites, fermented culture medium and a small amount of inactive yeast cells.

[0022] Anti-stress: refers to preventing and controlling stressful behaviors of animals. Stress refers to the stimulation and influence of stress factors that fish, shrimps, crabs and other farmed animals face in the process of modern aquaculture, such as water pollution, sudden changes in climate, long-distance transportation, etc. These stress reactions of varying degrees can not only directly affect the normal growth and development of animals, but also seriously lead to a decrease in the body's immunity of fish, shrimps and crabs, induce diseases and even death.

[0023] As mentioned in the background technology, the aquatic fermented feed in the prior art has a low promoting effect on the growth of aquatic animals. Therefore, in a first typical embodiment of the present application, an aquatic fermented feed auxiliary material is provided, including: a fermentation raw material and a fermentation strain composition, the mass of the fermentation strain composition accounts for 0.3%-0.5% of the mass of the fermentation raw material; wherein, in parts by weight, the fermentation raw material includes: 10-25 parts of peanut meal, 20-45 parts of soybean meal, 25-35 parts of bran and 5-15 parts of yeast metabolites; the fermentation strain composition includes: 45-55 parts of Saccharomyces boulardii, 10-15 parts of Bacillus subtilis, 2-4 parts of Lactobacillus plantarum, 14-18 parts of alkaline protease, 15-19 parts of glucose and 36-48 parts of water. Fermented feed additives with yeast metabolites can increase the nutrient content of the feed product. In order to further promote the accumulation of nutrients in aquatic fermented feed additives, fermented cultured bacteria and biological enzymes are introduced, which play a positive role in maintaining the survival ability of fish and shrimp and promoting the growth of fish and shrimp.

[0024] The boulardii yeast in the present application is an existing known or commercially available one (for example, the boulardii yeast with a deposit number of CCTCCNO.M2012116), which is mainly used for its ability to ferment fermentation raw materials to produce a variety of proteases, which can degrade pathogenic bacteria toxins and have excellent anti-stress, antioxidant and immune-enhancing effects on aquatic animals; in addition, Bacillus subtilis and Lactobacillus plantarum (commercially available products are suitable for the present application) can produce certain antibiotics, which are beneficial to maintaining the purity of the fermentation environment and the quality of the fermentation product, and both have a certain tolerance to temperature and can exert metabolic activity within a wide temperature range to obtain a fermentation product with high nutritional value and improve fermentation efficiency. Alkaline protease can efficiently catalyze the hydrolysis reaction of protein under alkaline conditions, thereby improving the nutritional content of the fermentation product.

[0025] In order to further efficiently obtain fermented materials with richer nutrients, in a preferred embodiment, the number of viable microorganisms in the fermentation strain composition is: Saccharomyces boulardii ≥ 10 billion CFU / g, Bacillus subtilis ≥ 2 billion CFU / g, Lactobacillus plantarum ≥ 2.5 billion CFU / g; preferably, the enzymatic activity of alkaline protease is ≥ 30,000 U / g.

[0026] In order to further efficiently ferment the fermentation raw materials to obtain fermentation products with higher nutrients, in a preferred embodiment, the sizes of peanut meal and soybean meal are independently 18-24 meshes.

[0027] In a second typical embodiment of the present application, a method for preparing the above-mentioned aquatic fermented feed auxiliary material is provided, the method comprising: S1, mixing Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water, performing activation culture, and obtaining an activated culture solution; S2, mixing the activated culture solution with peanut meal, soybean meal, bran and yeast metabolites, performing anaerobic fermentation culture, and obtaining the aquatic fermented feed auxiliary material.

[0028] By combining fermentation raw materials rich in yeast metabolites (i.e. peanut meal, soybean meal, bran and yeast metabolites) with a fermentation strain composition rich in Saccharomyces boulardii (i.e. including Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water) for anaerobic fermentation, macromolecular substances in the fermentation matrix can be decomposed into easily absorbable components, and aquatic fermented feed auxiliary materials with high nutritional value and rich nutrients can be obtained, which can promote the feeding and growth of aquatic animals, enhance the body's stress tolerance, disease resistance and antioxidant capacity of aquatic organisms, and can also regulate the intestinal flora balance and water quality of aquatic animals.

[0029] In order to further efficiently obtain a fermentation strain mixture with better activation effect, in a preferred embodiment, the temperature of the activation culture is 30° C.-40° C.; preferably, the activation culture time is 20-30 min.

[0030] In order to further efficiently ferment the fermentation raw materials to obtain fermentation products with more nutrients, in a preferred embodiment, the temperature of the anaerobic fermentation culture is 25°C-35°C; preferably, the time of the anaerobic fermentation culture is 72-96h.

[0031] If the auxiliary materials of aquatic fermented feed are wet materials, they are easy to absorb and digest, can provide more moisture, and are conducive to the growth and health of aquatic animals. However, they are relatively difficult to store and transport, and are prone to mold and deterioration. Therefore, there are dry materials that are easy to store and transport, not easy to deteriorate, and can be stored for a long time. However, dry materials are easy to spread in water, are not easily absorbed and digested by aquatic animals, and require a certain amount of time to soften and absorb water. In a preferred embodiment, the auxiliary materials of aquatic fermented feed include wet materials and dry materials.

[0032] In a preferred embodiment, the aquatic fermented feed auxiliary material obtained in S2 is a wet material, and the wet material is dried to obtain a dry material; preferably, the drying temperature is 50-55° C. In actual application, aquaculturists need to select a suitable feed form according to the needs and actual conditions of aquatic animals, or adopt a combination of wet and dry methods for feeding to ensure the health and growth of aquatic animals.

[0033] In a third typical embodiment of the present application, there is provided an application of the above-mentioned aquatic fermented feed auxiliary material or the aquatic fermented feed auxiliary material prepared by the above-mentioned method in aquaculture.

[0034] In order to efficiently utilize the aquatic fermented feed auxiliary materials of the present application and improve the good growth state of aquatic animals, in a preferred embodiment, the addition amount of the aquatic fermented feed auxiliary materials is 100-200 kg of wet material and 100-200 kg of dry material are added independently to each ton of commercial feed.

[0035] The present application is further described in detail below in conjunction with specific embodiments. These embodiments should not be construed as limiting the scope of protection claimed in the present application.

[0036] The boulardii Saccharomyces cerevisiae used in this application is derived from the China Center for Type Culture Collection (the collection number is CCTCCNO.M2012116, which has been disclosed in the patent disclosure document CN103374531A), Wuhan Lvjing Fenghua Biotechnology Co., Ltd. or Shandong Yihao Biotechnology Co., Ltd.; Bacillus subtilis is derived from Angel Yeast Co., Ltd., Shandong Yihao Biotechnology Co., Ltd. or Henan Jinxiang Biotechnology Co., Ltd.; Lactobacillus plantarum is derived from Ningbo Testo Biotechnology Co., Ltd., Shaanxi Baichuan Biotechnology Co., Ltd. or Guangzhou Huihe Biotechnology Co., Ltd.; alkaline protease is derived from Shandong Yaotu Bioengineering Co., Ltd. or Jiangsu Ruiyang Biotechnology Co., Ltd.; and Saccharomyces cerevisiae is derived from Angel Yeast Co., Ltd.

[0037] Example 1

[0038] The fermentation raw materials are prepared by weight as follows: 20 parts of peanut meal, 35 parts of soybean meal, 15 parts of yeast metabolites, and 30 parts of bran, which are evenly mixed; the fermentation strain composition is prepared by weight as follows: 50 parts of Saccharomyces boulardii, 13 parts of Bacillus subtilis, 3 parts of Lactobacillus plantarum, 17 parts of alkaline protease, 17 parts of glucose, and 36 parts of water. The mass of the fermentation strain composition accounts for 0.3% of the mass of the fermentation raw materials.

[0039] The number of live bacteria of the microorganisms in the fermentation strain composition is: Saccharomyces boulardii ≥ 10 billion CFU / g, Bacillus subtilis ≥ 2 billion CFU / g, Lactobacillus plantarum ≥ 2.5 billion CFU / g; the enzyme activity of alkaline protease is ≥ 30,000 U / g. The size of peanut meal and soybean meal is 18 mesh.

[0040] The method for preparing aquatic fermented feed auxiliary materials comprises the following steps:

[0041] S1, mixing Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water for activation culture, culturing at 30° C. for 30 minutes to obtain an activated culture solution.

[0042] S2, mixing the activated culture solution with peanut meal, soybean meal, bran and yeast metabolites, placing the mixture in a fermentation bag, and performing anaerobic fermentation culture at 30° C. for 72 hours to obtain aquatic fermented feed auxiliary materials.

[0043] The product's moisture, pH, viable bacteria count, crude protein, acid-soluble protein, lactic acid and other physical and chemical indicators are tested. If qualified, it can be directly bagged to make wet finished products of aquatic fermented feed auxiliary materials, or it can be dried at 50℃ to make dry finished products.

[0044] Example 2

[0045] The fermentation raw materials are prepared by weight as follows: 10 parts of peanut meal, 45 parts of soybean meal, 15 parts of yeast metabolites, and 30 parts of bran, which are evenly mixed; the fermentation strain composition is prepared by weight as follows: 45 parts of Saccharomyces boulardii, 15 parts of Bacillus subtilis, 4 parts of Lactobacillus plantarum, 18 parts of alkaline protease, 18 parts of glucose, and 40 parts of water. The mass of the fermentation strain composition accounts for 0.5% of the mass of the fermentation raw materials.

[0046] The number of live bacteria of the microorganisms in the fermentation strain composition is: Saccharomyces boulardii ≥ 10 billion CFU / g, Bacillus subtilis ≥ 2 billion CFU / g, Lactobacillus plantarum ≥ 2.5 billion CFU / g; the enzyme activity of alkaline protease is ≥ 30,000 U / g. The size of peanut meal and soybean meal is 20 mesh.

[0047] The method for preparing aquatic fermented feed auxiliary materials comprises the following steps:

[0048] S1, mixing Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water for activation culture, culturing at 35° C. for 25 minutes to obtain an activated culture solution.

[0049] S2, mixing the activated culture solution with peanut meal, soybean meal, bran and yeast metabolites, placing the mixture in a fermentation bag, and performing anaerobic fermentation culture at 35° C. for 80 hours to obtain aquatic fermented feed auxiliary materials.

[0050] The product's moisture, pH, viable bacteria count, crude protein, acid-soluble protein, lactic acid and other physical and chemical indicators are tested. If qualified, it can be directly bagged to make wet finished products of aquatic fermented feed auxiliary materials, or it can be dried at 55℃ to make dry finished products.

[0051] Example 3

[0052] The fermentation raw materials are prepared by weight as follows: 25 parts of peanut meal, 25 parts of soybean meal, 15 parts of yeast metabolites, and 35 parts of bran, which are evenly mixed; the fermentation strain composition is prepared by weight as follows: 55 parts of Saccharomyces boulardii, 10 parts of Bacillus subtilis, 2 parts of Lactobacillus plantarum, 17 parts of alkaline protease, 16 parts of glucose, and 48 parts of water. The mass of the fermentation strain composition accounts for 0.4% of the mass of the fermentation raw materials.

[0053] The number of live bacteria of the microorganisms in the fermentation strain composition is: Saccharomyces boulardii ≥ 10 billion CFU / g, Bacillus subtilis ≥ 2 billion CFU / g, Lactobacillus plantarum ≥ 2.5 billion CFU / g; the enzyme activity of alkaline protease is ≥ 30,000 U / g. The size of peanut meal and soybean meal is 24 mesh.

[0054] The method for preparing aquatic fermented feed auxiliary materials comprises the following steps:

[0055] S1, mixing Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water for activation culture at 40° C. for 20 minutes to obtain an activated culture solution.

[0056] S2, mixing the activated culture solution with peanut meal, soybean meal, bran and yeast metabolites, placing the mixture in a fermentation bag, and performing anaerobic fermentation culture at 25° C. for 96 hours to obtain aquatic fermented feed auxiliary materials.

[0057] The product's moisture, pH, viable bacteria count, crude protein, acid-soluble protein, lactic acid and other physical and chemical indicators are tested. If qualified, it can be directly bagged to make wet finished products of aquatic fermented feed auxiliary materials, or it can be dried at 55℃ to make dry finished products.

[0058] Comparative Example 1

[0059] Except that the yeast species in the fermentation strain composition is different from that in Example 1, the other aspects are the same. The yeast used in this comparative example 1 is Saccharomyces cerevisiae.

[0060] Comparative Example 2

[0061] Except that the yeast metabolites in the fermentation raw material were replaced by corn flour, which was different from Example 1, everything else was the same.

[0062] Comparative Example 3

[0063] Except that the number of each component of the fermentation raw material is different from that in Example 1, the others are the same. The fermentation raw material in this comparative example 3 is specifically:

[0064] The fermentation raw materials are mixed uniformly in a weight ratio of 25 parts of peanut meal, 36 parts of soybean meal, 3 parts of yeast metabolites and 36 parts of bran.

[0065] Comparative Example 4

[0066] Except that the number of components of the fermentation strain composition is different from that in Example 1, the others are the same. The fermentation raw materials in Comparative Example 4 are specifically:

[0067] The fermentation strain composition comprises 30 parts of Saccharomyces boulardii, 30 parts of Bacillus subtilis, 4 parts of Lactobacillus plantarum, 18 parts of alkaline protease, 18 parts of glucose and 42 parts of water in proportion by weight. The mass of the fermentation strain composition accounts for 0.3% of the mass of the fermentation raw material.

[0068] Test and detection:

[0069] Effects of aquatic fermented feed supplements on the survival rate and growth of crayfish

[0070] Test method: 720 crayfish were selected and divided into 8 groups, each with 3 parallel groups and 30 crayfish in each parallel group, which were the control group, Examples 1-3 and Comparative Examples 1-4.

[0071] The control group was fed with commercially available complete crayfish feed (Wuhan Tongwei Feed Co., Ltd.) for 15 consecutive days.

[0072] Example 1, Example 3, Comparative Example 1 and Comparative Example 2 were fed continuously for 15 days. The feed was prepared by adding 150 kg of aquatic fermented feed auxiliary wet material of each example or comparative example to per ton of commercially available crayfish complete feed.

[0073] Example 2, Comparative Example 3 and Comparative Example 4 were fed continuously for 15 days. The feed was prepared by adding 200 kg of dry aquatic fermented feed auxiliary materials of each example or comparative example to per ton of commercially available crayfish complete feed.

[0074] The other culture conditions were exactly the same.

[0075] The test was carried out in a 0.8×0.3×0.6m glass tank with circulating water oxygenation equipment, a water temperature of 24°C-26°C, a water depth of 0.6m, and the dissolved oxygen in the water was maintained at 7.78±0.13mg / L during the test. The test period was 15d, and the crayfish were managed according to the requirements of the intensive breeding pond on a daily basis without special management. During the test, the feeding amount was 3% of the crayfish body weight. The embodiment, comparative example and control group were all fed in equal amounts each time, and the feeding frequency was 2 times / d (8:00-9:00 in the morning and 17:00-18:00 in the evening), and the feeding amount in the morning and evening was 30% and 70% respectively. 12h light and 12h dark every day. The number of survivors and weight gain were recorded during the breeding period.

[0076] Experimental phase: The experimental period was from May 5 to May 20, a total of 15 days. On the 1st and 15th days of the experiment, 30 crayfish were randomly selected from each group, and the total weight and shrimp meat weight were weighed to calculate the weight growth rate, survival rate and meat yield.

[0077] The calculation formula is as follows:

[0078] Weight gain rate (%) = ((weight at the end of the test - weight at the beginning of the test) / weight at the beginning of the test) × 100%;

[0079] Survival rate (%) = (last digit / initial digit) × 100%;

[0080] Meat yield (%) = (weight of shrimp meat at the end of the test / total weight of shrimp at the end of the test) × 100%.

[0081] On the first day of the experiment and after the 15th day of the experiment, the crayfish of the embodiment and the control group were taken out, weighed and the survival rate was calculated. The results are shown in Table 1:

[0082] Table 1 Results of weight gain rate, survival rate and meat yield of crayfish before and after the experiment

[0083] Group Weight gain rate Survival rate Meat yield Control group 43.7±2.5％ 34.5±1.2％ 12.0±0.5％ Example 1 108.5±3.0％ 40.6±1.7％ 16.1±0.9％ Example 2 140.6±1.6％ 47.8±0.7％ 16.2±0.8％ Example 3 133.5±1.1％ 45.7±1.0％ 15.8±0.8％ Comparative Example 1 88.7±3.3％ 38.8±1.1％ 13.3±0.1％ Comparative Example 2 94.6±2.7％ 37.9±0.5％ 13.9±0.4％ Comparative Example 3 100.7±1.8％ 39.3±0.6％ 14.2±0.6％ Comparative Example 4 95.8±0.5％ 37.7±1.4％ 13.7±0.5％

[0084] From the feeding results in Table 1, it can be seen that the weight gain rate, survival rate and meat yield of the embodiment are higher than those of the control group and the comparative example; the weight gain rate, survival rate and meat yield of Example 2 are the highest, especially the weight gain rate is significantly higher than that of the control group. Overall, as the proportion of aquatic fermented feed auxiliary materials of the present application increases, its growth rate and survival rate gradually increase, and the meat yield is good, indicating that feeding the aquatic fermented feed auxiliary materials of the present application has a good effect on the survival, growth and quality of crayfish.

[0085] From the above description, it can be seen that the above-mentioned embodiments of the present invention achieve the following technical effects: yeast metabolites and yeast-derived functional substances such as Saccharomyces boulardii, which are rich in various biologically active substances such as amino acids, organic acids, fulvic acid, polysaccharides and large and medium trace elements, are added to the aquatic fermented feed auxiliary materials of the present application, and solid-state anaerobic fermentation is carried out. It has high nutritional value and rich nutrients, has a good appetite for aquatic animals, and promotes the growth of aquatic animals; and the products after yeast fermentation can degrade pathogenic bacteria toxins to regulate the balance of intestinal flora of aquatic animals, enhance their anti-stress and antioxidant capabilities, and can further purify pond water quality and reduce pollution.

[0086] In addition, the fermented strain composition of the present application can decompose the macromolecular substances in the fermentation matrix into easily absorbable components, thereby further improving the nutritional value of the aquatic culture and having a good growth-promoting effect on aquatic animals.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An aquatic fermented feed auxiliary material, characterized in that: include: A fermentation raw material and a fermentation strain composition, wherein the mass of the fermentation strain composition is 0.3%-0.5% of the mass of the fermentation raw material; Wherein, the fermentation raw materials include, by weight: 10-25 parts of peanut meal, 20-45 parts of soybean meal, 25-35 parts of bran and 5-15 parts of yeast metabolites; The fermented bacteria composition comprises: 45-55 parts of Saccharomyces boulardii, 10-15 parts of Bacillus subtilis, 2-4 parts of Lactobacillus plantarum, 14-18 parts of alkaline protease, 15-19 parts of glucose and 36-48 parts of water.

2. The aquatic fermented feed auxiliary material according to claim 1, characterized in that: The viable counts of the microorganisms in the fermented bacteria composition are: Saccharomyces boulardii ≥ 10 billion CFU / g, Bacillus subtilis ≥ 2 billion CFU / g, and Lactobacillus plantarum ≥ 2.5 billion CFU / g.

3. The aquatic fermented feed supplementary material according to claim 1, characterized in that: The enzymatic activity of the alkaline protease is ≥30000 U / g.

4. The aquatic fermented feed supplementary material according to claim 1, characterized in that: The diameters of the peanut meal and the soybean meal are independently 18-24 meshes.

5. A method for preparing the aquatic fermented feed auxiliary material according to any one of claims 1 to 4, characterized in that: The method comprises: S1, mixing Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, alkaline protease, glucose and water for activation culture to obtain an activated culture solution; S2, mixing the activated culture solution with peanut meal, soybean meal, bran and yeast metabolites, and performing anaerobic fermentation culture to obtain the aquatic fermented feed auxiliary material.

6. The method according to claim 5, characterized in that The activation culture temperature is 30°C-40°C; Preferably, the activation culture time is 20-30 min.

7. The method according to claim 5, characterized in that The temperature of the anaerobic fermentation culture is 25°C-35°C; Preferably, the anaerobic fermentation culture time is 72-96 hours.

8. The method according to claim 5, characterized in that The aquatic fermented feed auxiliary material includes wet material and dry material, wherein the aquatic fermented feed auxiliary material obtained in S2 is the wet material, and the dry material is obtained by drying the wet material; Preferably, the drying temperature is 50-55°C.

9. Use of the aquatic fermented feed auxiliary material according to any one of claims 1 to 4 or the aquatic fermented feed auxiliary material prepared by the method according to any one of claims 5 to 8 in aquaculture.

10. The use according to claim 9, characterized in that: The amount of the aquatic fermented feed auxiliary material added is 100-200 kg of wet material and 100-200 kg of dry material added independently to each ton of commercial feed.