A fluorescence imaging method suitable for bladder photodynamic diagnosis

Abstract

The present application provides a fluorescence imaging method suitable for bladder photodynamic diagnosis, the method comprising: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observation tissue, and then the spectrum of the green fluorescence band generated by the excitation of riboflavin is filtered through a notch filter to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the red, green, and blue light sources are turned on at the same time, the light is reflected by the observation tissue, and then the green fluorescence band spectrum is absorbed by the notch filter, and the illumination light of the green fluorescence band is enhanced by the light compensation module, so that the light after passing through the notch filter is white light. By combining the notch filter and the light compensation module, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, and the color restoration of white light imaging can be guaranteed at the same time.

Description

Technical Field

[0001] The invention relates to the field of photodynamic diagnosis, and in particular to a fluorescence imaging method suitable for bladder photodynamic diagnosis. Background Art

[0002] Photodynamic diagnosis (PDD) is a new diagnostic technology that allows photosensitizing substances to accumulate in diseased tissues and irradiates them with excitation light to make them fluoresce. The presence of disease and the diseased tissues can be determined by observing and detecting fluorescence.

[0003] This technology is widely used in the field of medical diagnosis. For example, in the diagnosis of tumors, photosensitizers are selectively enriched in cancer cells; then the cancer tissue is irradiated with excitation light to make the enriched photosensitizers produce fluorescence; by analyzing the intensity and distribution of the fluorescence, the location and condition of the cancer can be determined. For another example, in the process of bladder PDD imaging, blue-violet light is used as the excitation light to irradiate the photosensitizer-aggregated tissue, stimulating red fluorescence and imaging it.

[0004] However, during bladder PDD imaging, the metabolite riboflavin in urine will be excited by the excitation light and emit green fluorescence, thereby interfering with the contrast of PDD fluorescence. The existing method is to change the excitation light band, such as using 500nm as the photosensitizer excitation wavelength to reduce the excitation efficiency of riboflavin. However, changing the excitation wavelength will also cause the fluorescence excitation efficiency of the photosensitizer to decrease, and the fluorescence brightness of PDD imaging will decrease. At the same time, it will cause more loss of visible light imaging band, which is not conducive to traditional white light imaging. Summary of the invention

[0005] The present application provides a fluorescence imaging method suitable for bladder photodynamic diagnosis. By combining a notch filter and a light compensation module, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, while the color restoration of traditional white light imaging is also ensured.

[0006] In view of this, the first aspect of the present application provides a fluorescence imaging method suitable for bladder photodynamic diagnosis, characterized in that the method is applied to a fluorescence imaging device for bladder photodynamic diagnosis, and the fluorescence imaging device for bladder photodynamic diagnosis includes at least two modes of photodynamic diagnosis PDD fluorescence mode and photodynamic white light imaging mode. The method includes: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue, and then the spectrum of the green fluorescence band generated by the excitation of riboflavin is filtered through a notch filter to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the red, green and blue light sources are turned on at the same time, the light is reflected by the observed tissue, and then the green fluorescence band spectrum is absorbed by the notch filter, and the illumination light of the green fluorescence band is enhanced by the light compensation module to restore the spectrum absorbed by the notch filter, so that the light after passing through the notch filter is white light.

[0007] In combination with the first aspect, in a possible implementation, the optical compensation module is a bandpass filter, and for light with a wavelength of λ, a standard deviation of the transmittance of the notch filter and the bandpass filter is less than or equal to a set standard deviation threshold.

[0008] In combination with the first aspect, in a possible implementation, for light with a wavelength of λ, a standard deviation s of the summed transmittance of the notch filter and the bandpass filter is:

[0009] ;

[0010] in, The wavelength is The same transmittance of light, is the mean value of the same and light transmittance;

[0011] ;

[0012] ,in, The wavelength is The transmittance of light entering the notch filter, The wavelength is The transmittance of light entering the bandpass filter.

[0013] In combination with the first aspect, in a possible implementation, the optical compensation module is connected to the red, green, and blue light sources and is used to adjust the optical power of the red, green, and blue light sources. The optical compensation module adjusts the light output ratio of the red, green, and blue lights according to a preset optical power ratio, wherein the ratio of the red, green, and blue lights in the preset optical power ratio is related to the transmittance of the notch filter.

[0014] In combination with the first aspect, in a possible implementation method, the ratio of the red, green and blue colors of the pre-set optical power ratio is related to the transmittance of the notch filter, specifically including: if it is determined that the transmittance ratio of the notch filter for the red, green and blue light is a:b:a, then the output power of the red light spectrum, the green light spectrum and the blue light spectrum is controlled by the optical compensation module to be b:a:b, and a and b are both positive numbers.

[0015] In combination with the first aspect, in a possible implementation, the optical powers of red light, green light, and blue light emitted by the red, green, and blue light sources entering the photosensitive element are respectively:

[0016] ;

[0017] ;

[0018] ,

[0019] in, is the light power of red light entering the photosensitive element, is the optical power of green light entering the photosensitive element, is the optical power of blue light entering the photosensitive element, is the wavelength of light, is the output power of the red light spectrum, is the output power of the green light spectrum, is the output power of the blue light spectrum.

[0020] In combination with the first aspect, in a possible implementation, in the photodynamic white light imaging mode, the light compensation module controls the output power of the red, green and blue lights so that the difference percentage between the red, green and blue light powers entering the photosensitive element and the average power of the three lights is less than or equal to a preset difference percentage threshold.

[0021] In combination with the first aspect, in a possible implementation, the average power of the red, green and blue lights is for:

[0022] ;

[0023] The percentage difference between the red light power and the average power of the three-color light is:

[0024] ;

[0025] The percentage difference between the green light power and the average power of the three-color light is:

[0026] ;

[0027] The percentage difference between the blue light power and the average power of the three colors of light is:

[0028] ;

[0029] in, is the red light power, is the green light power, is the blue light power, is the difference between the red light power and the average power of the three colors of light, is the difference between the green light power and the average power of the three colors of light, is the difference between the blue light power and the average power of the three colors of light, is the preset difference percentage threshold, .

[0030] In combination with the first aspect, in a possible implementation, the light compensation module controls the red, green and blue light sources to perform imaging in a stroboscopic manner, and controls the ratio of the light powers of the red, green and blue light sources, so that the red, green and blue lights are reflected by the observed tissue and synthesized into white light after passing through the notch filter.

[0031] In combination with the first aspect, in a possible implementation manner, the light compensation module controls the red, green and blue light sources to perform staggered imaging according to a preset frame rate.

[0032] In combination with the first aspect, in a possible implementation manner, the light compensation module controls the red and blue light sources to be turned on synchronously, and the blue and green light sources to be turned on asynchronously.

[0033] In combination with the first aspect, in a possible implementation method, the controlling the ratio of the optical powers of the red, green and blue light sources specifically includes: the optical compensation module adjusts the light output ratio of the red, green and blue light sources according to a preset optical power ratio, wherein the preset optical power ratio is related to the transmittance of the notch filter.

[0034] In combination with the first aspect, in a possible implementation method, the pre-set optical power ratio is related to the transmittance of the notch filter and specifically includes: if it is determined that the transmittance ratio of the notch filter for red, green and blue light is a:b:a, then the output power of the red light spectrum, the green light spectrum and the blue light spectrum is controlled by the optical compensation module to be b:a:b, and a and b are both positive numbers.

[0035] In combination with the first aspect, in a possible implementation, the optical powers of red light, green light, and blue light emitted by the red, green, and blue light sources entering the photosensitive element are respectively:

[0036] ;

[0037] ;

[0038] ,

[0039] in, is the light power of red light entering the photosensitive element, is the optical power of green light entering the photosensitive element, is the optical power of blue light entering the photosensitive element, is the wavelength of light, is the output power of the red light spectrum, The output power of the green spectrum, is the output power of the blue light spectrum.

[0040] In combination with the first aspect, in a possible implementation, in the photodynamic white light imaging mode, the light compensation module controls the output power of the red, green and blue light sources so that the difference percentage between the red, green and blue light powers entering the photosensitive element and the average power of the three colors of light is less than or equal to a preset difference percentage threshold.

[0041] In combination with the first aspect, in a possible implementation, the average power of the red, green and blue lights is for:

[0042] ;

[0043] The percentage difference between the red light power and the average power of the three-color light is:

[0044] ;

[0045] The percentage difference between the green light power and the average power of the three-color light is:

[0046] ;

[0047] The percentage difference between the blue light power and the average power of the three colors of light is:

[0048] ;

[0049] in, is the red light power, is the green light power, is the blue light power, is the difference between the red light power and the average power of the three colors of light, is the difference between the green light power and the average power of the three colors of light, is the difference between the blue light power and the average power of the three colors of light, is the preset power difference percentage threshold, .

[0050] The present application provides a fluorescence imaging method suitable for bladder photodynamic diagnosis, characterized in that the method is applied to a fluorescence imaging device for bladder photodynamic diagnosis, and the fluorescence imaging device for bladder photodynamic diagnosis includes at least two modes of photodynamic diagnosis PDD fluorescence mode and photodynamic white light imaging mode. The method includes: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue and then filtered through a notch filter to filter the spectrum of the green fluorescence band generated by the excitation of riboflavin to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the red, green and blue light sources are turned on at the same time, the light is reflected by the observed tissue and then absorbed by the notch filter to absorb the green fluorescence band spectrum, and the illumination light of the green fluorescence band is enhanced by a light compensation module to restore the spectrum absorbed by the notch filter, so that the light after passing through the notch filter is white light. By combining the notch filter and the light compensation module, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, while the color restoration of traditional white light imaging can be guaranteed. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 A schematic diagram of a flow chart of a fluorescence imaging method suitable for bladder photodynamic diagnosis in an embodiment of the present application;

[0052] Figure 2 A schematic diagram of a fluorescence imaging device for bladder photodynamic diagnosis in an embodiment of the present application;

[0053] Figure 3 A schematic diagram of a fluorescence imaging device for bladder photodynamic diagnosis in an embodiment of the present application;

[0054] Figure 4 This is a schematic diagram of frame-shifted imaging of white light synthesized by red, green and blue light in a photodynamic white light imaging mode in an embodiment of the present application. DETAILED DESCRIPTION

[0055] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of this application.

[0056] The term "and / or" in this application can be a description of the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this application generally indicates that the associated objects before and after are in an "or" relationship.

[0057] The terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable where appropriate, so that the embodiments described herein can be implemented in an order other than that illustrated or described herein. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or modules is not necessarily limited to those steps or modules that are clearly listed, but may include other steps or modules that are not clearly listed or inherent to these processes, methods, products or devices.

[0058] During bladder PDD imaging, the metabolite riboflavin in urine will be excited by the excitation light and emit green fluorescence, thereby interfering with the contrast of PDD fluorescence. The existing method is to reduce the excitation efficiency of riboflavin by changing the excitation light band, such as using 500nm as the photosensitizer excitation wavelength. However, changing the excitation wavelength will also cause the fluorescence excitation efficiency of the photosensitizer to decrease, and the fluorescence brightness of PDD imaging will decrease. At the same time, it will cause more loss of visible light imaging band, which is not conducive to traditional white light imaging.

[0059] Therefore, the present application provides a fluorescence imaging method for bladder photodynamic diagnosis. By combining a filtering module and a light compensation module, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, while the color restoration of traditional white light imaging can be ensured.

[0060] See also Figure 1 The present application provides a fluorescence imaging method for bladder photodynamic diagnosis, the method comprising:

[0061] 101. Determine the working mode.

[0062] The fluorescence imaging method for bladder photodynamic diagnosis provided in this application is applied to a fluorescence imaging device for bladder photodynamic diagnosis, in which a notch filter and a light compensation module are provided. The device has two modes: PDD fluorescence mode and photodynamic white light imaging mode. The device may also add other modes in the future, which are not limited here.

[0063] 102. In the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue and then filtered through the notch filter to filter the spectrum of the green fluorescence band produced by the excitation of riboflavin, so as to reduce the interference of green fluorescence on the PDD fluorescence contrast.

[0064] When the working mode is determined to be the PDD fluorescence mode, the blue light source is turned on, and the blue light is reflected by the observed tissue to excite the fluorescence of riboflavin in the bladder. The PDD fluorescence and the spectrum of the green fluorescence band generated by the excited riboflavin are collected by the imaging lens group.

[0065] The notch filter can be used to filter the spectrum of the green fluorescence band generated by the excitation of riboflavin to reduce the interference of green fluorescence on the PDD fluorescence contrast. The PDD fluorescence passing through the notch filter reaches the photosensitive element. The photosensitive element then converts the optical signal into an electrical signal and transmits it to the image processor for processing, thereby outputting a PDD fluorescence image.

[0066] 103. In the photodynamic white light imaging mode, when the red, green and blue light sources are turned on at the same time, the light is reflected by the observed tissue, absorbs the green fluorescence band spectrum through the notch filter, and enhances the illumination light of the green fluorescence band through the light compensation module to restore the spectrum absorbed by the notch filter, so that the light after passing through the notch filter is white light.

[0067] In this photodynamic white light imaging mode, when the red, green, and blue light sources are turned on at the same time, the light is reflected by the observed tissue, absorbs the green fluorescence band spectrum through the notch filter, and enhances the illumination light in the green fluorescence band through the light compensation module to restore the spectrum absorbed by the notch filter, so that the light after passing through the notch filter is white light.

[0068] Specifically, in one implementation, see Figure 2 The fluorescence imaging device for bladder photodynamic diagnosis includes: a blue light source 11, an imaging lens group 12, a notch filter 13, a photosensitive element 14, a white light source 15, a bandpass filter 16, and an image processor 17.

[0069] In the photodynamic white light imaging mode, the white light source 15 emits white light, and the bandpass filter 16 enhances the spectrum of the green fluorescence band in the white light. After passing through the bandpass filter 16, the white light is irradiated to the observed tissue and is reflected by the observed tissue to the imaging lens group 12. The imaging lens group 12 is used to collect the white light reflected by the observed tissue and transmit it to the notch filter 13. The notch filter 13 filters the spectrum of the green fluorescence band and transmits it to the photosensitive element 14. The photosensitive element 14 then converts the optical signal into an electrical signal and transmits it to the image processor 17 for processing, thereby outputting a white light image.

[0070] The difference between the absorption amount of the spectrum of the green fluorescence band by the notch filter 13 and the increase amount of the spectrum of the green fluorescence band by the bandpass filter 16 is within a preset range. Exemplarily, the difference between the absorption amount of the spectrum of the green fluorescence band by the notch filter 13 and the increase amount of the spectrum of the green fluorescence band by the bandpass filter 16 can be within 110%, which is only an example and is not limited here.

[0071] It should be noted that the notch filter 13 is highly transparent to the blue band spectrum and the red band spectrum, and partially filters the green band spectrum. Specifically, the transmittance of the notch filter 13 for the blue band spectrum and the red band spectrum exceeds the first preset transmittance threshold. And the transmittance for the green band spectrum is lower than the second preset transmittance threshold. Exemplarily, the first preset transmittance threshold and the second preset transmittance threshold can be set to 90% and 110%, respectively. It can also be set to 150%, 160%, etc., which can be set according to actual business needs and is not limited here. The setting of the transmittance can be achieved by coating the surface of the notch filter. The green band spectrum filtered by the notch filter is between 500 nanometers and 575 nanometers.

[0072] The bandpass filter 16 is highly transparent to the green band spectrum, while partially filtering the blue band spectrum and the red band spectrum. Specifically, the transmittance of the bandpass filter 16 for the green band spectrum exceeds the third preset transmittance threshold, while filtering the blue band spectrum and the red band spectrum is lower than the fourth transmittance threshold. Exemplarily, the third preset transmittance threshold and the fourth preset transmittance threshold can be set to 90% and 110%, respectively. It can also be set to 150%, 160%, etc., which can be set according to actual business needs and is not limited here. The setting of the transmittance can be achieved by coating the surface of the bandpass filter.

[0073] It should be noted that the transmittance of the notch filter 13 and the bandpass filter 16 for light with a wavelength of λ is complementary. Specifically, for light with a wavelength of λ, the standard deviation of the transmittance of the notch filter 13 and the bandpass filter 16 is less than or equal to a set standard deviation threshold. Exemplarily, the standard deviation threshold can be set to 110%, but is not limited thereto.

[0074] For light with a wavelength of λ, the standard deviation s of the transmittance of the notch filter 13 and the bandpass filter 16 is:

[0075] ;

[0076] in, The wavelength is The same transmittance of light, is the mean value of the same and light transmittance;

[0077] ;

[0078] in, The wavelength is The transmittance of the light entering the notch filter 13, The wavelength is The transmittance of the light entering the bandpass filter 16.

[0079] Among them, 400 represents a wavelength of 400nm, 680 represents a wavelength of 680nm, and the wavelength of 400~680nm usually represents the visible light band. 281 means that there is one data for every 1nm from 400~680nm, for a total of 281 data.

[0080] In the second implementation, see Figure 3 The fluorescence imaging device for bladder photodynamic diagnosis comprises: a blue light source 21 , a green light source 22 , a red light source 23 , a light compensation module 24 , an imaging lens group 25 , a notch filter 26 , a photosensitive element 27 , and an image processor 28 .

[0081] In the photodynamic white light imaging mode, the blue light source 21, the green light source 22, and the red light source 23 are all turned on under the control of the light compensation module 24, and the red, green, and blue light emitted by the blue light source 21, the green light source 22, and the red light source 23 are all adjusted according to the preset light power by the light compensation module 24. After the three-color light is reflected by the observed tissue, the reflected light is collected by the imaging lens group 25, and after being filtered by the notch filter 26, it enters the photosensitive element 27. The photosensitive element 27 is used to convert the optical signal into an electrical signal and transmit it to the image processor 8 for processing, and output a white light image.

[0082] The optical compensation module 24 controls the optical powers output by the blue light source 21 and the red light source 23 to be consistent, and the output power of the green light source 22 is higher than the optical power of the blue light source 21 or the red light source 23 .

[0083] It should be noted that the optical compensation module 24 adjusts the light output ratio of red, green and blue light according to a preset optical power ratio, wherein the ratio of red, green and blue in the preset optical power ratio is related to the transmittance of the notch filter 26 .

[0084] Furthermore, if it is determined that the transmittance ratio of the notch filter 26 for the red, green and blue light is a:b:a, then the output power of the red light spectrum, the green light spectrum and the blue light spectrum is controlled by the light compensation module 4 to be b:a:b, where a and b are both positive numbers. For example, if it is determined that the transmittance of the notch filter 26 for the red, green and blue light is 1:0.4:1 respectively. Then it can be determined that the output power of the red, green and blue light controlled by the light compensation module 24 is 0.4:1:0.4. This is only an example and is not a limitation of the present application.

[0085] It should be noted that the optical powers of red light, green light, and blue light emitted by the red, green, and blue light sources entering the photosensitive element are respectively:

[0086] ;

[0087] ;

[0088] ,

[0089] in, is the light power of red light entering the photosensitive element, is the optical power of green light entering the photosensitive element, is the optical power of blue light entering the photosensitive element, is the wavelength of light, is the output power of the red light spectrum, is the output power of the green light spectrum, is the output power of the blue light spectrum, is the integral formula's decimal point.

[0090] It is understandable that in the photodynamic white light imaging mode, the light compensation module 24 controls the output power of the red, green and blue light so that the difference percentage between the red, green and blue light power entering the photosensitive element 27 and the average power of the three-color light is less than or equal to the preset difference percentage threshold. The difference percentage threshold can be set according to actual conditions, and can be set to 10% for example. This is only an example and is not a limitation of the present application.

[0091] Furthermore, the average power of the three colors of red, green and blue for:

[0092] ;

[0093] The percentage difference between the red light power and the average power of the three-color light is:

[0094] ;

[0095] The percentage difference between the green light power and the average power of the three-color light is:

[0096] ;

[0097] The percentage difference between the blue light power and the average power of the three colors of light is:

[0098] ;

[0099] in, is the red light power, is the green light power, is the blue light power, is the difference between the red light power and the average power of the three colors of light, is the difference between the green light power and the average power of the three colors of light, is the difference between the blue light power and the average power of the three colors of light, is the difference percentage threshold between the preset and the average power of the three-color light. . For example, It can be equal to 10%.

[0100] In the third implementation, please continue to refer to Figure 3 The fluorescence imaging device for bladder photodynamic diagnosis comprises: a blue light source 21 , a green light source 22 , a red light source 23 , a light compensation module 24 , an imaging lens group 25 , a notch filter 26 , a photosensitive element 27 , and an image processor 28 .

[0101] When it is determined that the working mode is the PDD fluorescence mode, the blue light source 21, the green light source 22, and the red light source 23 are imaged in a stroboscopic manner under the control of the light compensation module 24, and the red, green, and blue light emitted by the blue light source 21, the green light source 22, and the red light source 23 are all adjusted according to the preset light power by the light compensation module 24. The emitted light is reflected by the observed tissue and the reflected light is collected by the imaging lens group 25, and after being filtered by the notch filter 26, it enters the photosensitive element 27. The photosensitive element 27 is used to convert the optical signal into an electrical signal and transmit it to the image processor 28 for processing, and output a white light image.

[0102] The light compensation module 24 is used to control the red light, green light and blue light to form staggered images according to a preset frame rate. Specifically, the light compensation module 24 is used to control the blue light and red light to be on synchronously, and the blue light and green light to be on asynchronously. Furthermore, the light compensation module 24 is used to: when in the first frame, control the blue light to be off, the red light to be off, and the green light to be on at the same time; in the second frame, control the blue light to be on, the red light to be on, and the green light to be off at the same time; the third frame is the same as the first frame, the fourth frame is the same as the second frame...one cycle for every two frames.

[0103] The optical compensation module 24 is also used to adjust the light output ratio of red, green and blue light according to a preset optical power ratio, wherein the ratio of red, green and blue in the preset optical power ratio is related to the transmittance of the notch filter.

[0104] Specifically, if it is determined that the transmittance ratio of the notch filter 26 for the red, green and blue light is a:b:a, then the output power of the red light spectrum, the green light spectrum and the blue light spectrum controlled by the light compensation module 24 is b:a:b, and a and b are both positive numbers. Exemplarily, if it is determined that the transmittance of the notch filter 26 for the red, green and blue light is 1:0.4:1 respectively. Then it can be determined that the output power of the red, green and blue light controlled by the light compensation module 24 is 0.4:1:0.4. This is only an example and is not a limitation of the present application.

[0105] It should be noted that the light compensation module 24 controls the red, green and blue lights to be imaged in a stroboscopic manner and controls the ratio of the light powers of the red, green and blue lights so that in the white light mode, the image obtained by the photosensitive element 7 can be synthesized into white light. Figure 4 shown.

[0106] It should be noted that the optical powers of red light, green light, and blue light emitted by the red, green, and blue light sources entering the photosensitive element are respectively:

[0107] ;

[0108] ;

[0109] ,

[0110] in, is the light power of red light entering the photosensitive element, is the optical power of green light entering the photosensitive element, is the optical power of blue light entering the photosensitive element, is the wavelength of light, is the output power of the red light spectrum, is the output power of the green light spectrum, is the output power of the blue light spectrum.

[0111] It is understood that in the photodynamic white light imaging mode, the light compensation module 24 controls the output power of the red, green and blue light so that the difference percentage between the red, green and blue light power entering the photosensitive element and the average power of the three-color light is less than or equal to the preset difference percentage threshold. The difference percentage threshold can be set according to actual conditions, and can be set to 10% for example. This is only an example and is not a limitation of the present application.

[0112] Furthermore, the average power of the three colors of red, green and blue for:

[0113] ;

[0114] The percentage difference between the red light power and the average power of the three-color light is:

[0115] ;

[0116] The percentage difference between the green light power and the average power of the three-color light is:

[0117] ;

[0118] The percentage difference between the blue light power and the average power of the three colors of light is:

[0119] ;

[0120] in, is the red light power, is the green light power, is the blue light power, is the difference between the red light power and the average power of the three colors of light, is the difference between the green light power and the average power of the three colors of light, is the difference between the blue light power and the average power of the three colors of light, is the preset difference percentage threshold, .

[0121] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working processes of the systems, devices and units described above can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0122] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present application is essentially or the part that contributes to the prior art or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a storage medium, including several instructions to enable a computer device (which can be a personal computer, server, or network device, etc.) to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM), random access memory (RAM), disk or optical disk, etc., various media that can store program codes.

[0123] In the embodiments provided in this application, it should be understood that the disclosed methods can be implemented in other ways without exceeding the spirit and scope of this application. The current embodiment is only an illustrative example and should not be used as a limitation. The specific content given should not limit the purpose of this application. For example, some features can be ignored or not executed.

[0124] The technical means disclosed in the present application are not limited to the technical means disclosed in the above-mentioned implementation methods, but also include technical solutions composed of any combination of the above technical features. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of this application, and these improvements and modifications are also regarded as the protection scope of this application.

[0125] The above is a detailed introduction to a fluorescence imaging method suitable for bladder photodynamic diagnosis provided in the embodiments of the present application. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea; at the same time, for those of ordinary skill in the art, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as a limitation on the present application. Although the present application has been described in detail with reference to the aforementioned embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein; and these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A fluorescence imaging method suitable for bladder photodynamic diagnosis, characterized in that: The method is applied to a fluorescence imaging device for bladder photodynamic diagnosis, wherein the fluorescence imaging device for bladder photodynamic diagnosis includes at least two modes: a photodynamic diagnosis PDD fluorescence mode and a photodynamic white light imaging mode. The method includes: In the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue and then filtered through the notch filter to filter the spectrum of the green fluorescence band generated by the excitation of riboflavin, so as to reduce the interference of green fluorescence on the PDD fluorescence contrast; In the photodynamic white light imaging mode, when the red, green, and blue light sources are turned on at the same time, the light is reflected by the observed tissue, absorbs the green fluorescence band spectrum through the notch filter, and enhances the illumination light of the green fluorescence band through the light compensation module to restore the spectrum absorbed by the notch filter, so that the light after passing through the notch filter is white light; The light compensation module is a bandpass filter. The standard deviation s of the transmittance of the notch filter and the bandpass filter is less than or equal to the set standard deviation threshold; ； Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ； T(λ)=Tn(λ)×Tb(λ), where Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter, and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter.

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