A cell culture dish

Abstract

The present invention discloses a cell culture dish, belonging to the field of culture dishes, which includes a culture dish having a bearing cavity therein; a driving component, which includes a connecting frame, which is detachably mounted on the culture dish. The cell culture dish of the present invention can simultaneously trigger the stirring and mixing component to stir the mixed liquid and the linked scraping component to move up and down and scrape through the arranged driving component. After the driving motor drives the driving rod to rotate, the driving wheel is driven to rotate through the arranged stirring and mixing component, so that the driving wheel drives the driven wheel to rotate, and after the driven wheel rotates, the driven wheel drives the dragon blade to rotate, so as to mix and stir the aqueous solution and the cleaning liquid in the bearing cavity of the culture dish, and can simultaneously drive the mixed solution of the aqueous solution and the cleaning liquid to shake during the mixing and stirring process to enhance the flushing effect on the inner bottom wall and the inner side wall of the culture dish.

Description

Technical Field

[0001] The invention belongs to the field of culture dishes, and in particular relates to a cell culture dish. Background Art

[0002] Cells are the basic structural and functional units of living organisms. Cells are extremely small and can only be seen under a microscope. They have a variety of shapes. They are mainly composed of a nucleus and cytoplasm, with a cell membrane on the surface. Higher plants have a cell wall outside the cell membrane, often have plastids in the cytoplasm, chloroplasts and vacuoles in the body, and mitochondria. Animal cells have no cell walls, often have centrioles in the cytoplasm, but higher plant cells do not have them. Cells have functions such as movement, nutrition, and reproduction. A cell culture dish is a laboratory dish used for microbial or cell culture. It consists of a flat disc-shaped bottom and a lid, and is generally made of glass or plastic. There are basically two types of culture dish materials, mainly plastic and glass. Glass can be used for plant materials, microbial culture, and adherent culture of animal cells;

[0003] Each time a cell culture dish is used up, it is necessary to clean the dish before the next culture, otherwise it will affect the accuracy of cell culture. During the cleaning process, an aqueous solution and a cleaning solution are generally added to the dish first. After the cleaning solution and the aqueous solution are mixed, the dish is cleaned. However, it is often difficult to clean and flush the inner side wall and the inner bottom wall of the dish during cleaning. During cleaning, the cleaning solution is often difficult to mix quickly with the aqueous solution, resulting in low cleaning efficiency. Improvements are urgently needed.

[0004] The present invention seeks to alleviate or at least mitigate such problems or deficiencies by providing new or otherwise improved culture dishes. Summary of the invention

[0005] In view of one or more of the above defects or improvement needs of the prior art, the present invention provides a cell culture dish, which has the advantages of being able to simultaneously clean and flush the inner side wall and the inner bottom wall of the culture dish and being able to further promote the mixing of the cleaning liquid and the aqueous solution during the cleaning process to enhance the cleaning effect.

[0006] To achieve the above-mentioned object, the present invention provides a cell culture dish, which includes a culture dish having a bearing cavity therein; a driving member, which includes a connecting frame, which is detachably mounted on the culture dish, on which a set of disks is detachably arranged, on which a driving motor is arranged, and the output end of the driving motor can be inserted into the set of disks, on which a driving rod is detachably arranged, and on which a first bevel gear and a driving wheel are removably arranged in sequence;

[0007] A connecting seat, which is detachably mounted on the sleeve disc, in which a linkage rod is rotatably arranged, a second bevel gear meshing with the first bevel gear is detachably arranged on one end of the linkage rod, and a cam is detachably arranged on the other end of the linkage rod;

[0008] A stirring and mixing member, which is detachably arranged on the sleeve disc, meshes with the driving wheel, and can penetrate into the bearing cavity of the culture dish;

[0009] A liquid mixing member, which is detachably mounted on the culture dish and can move along with the stirring and mixing member when the stirring and mixing member moves, and is used for feeding liquid into the bearing cavity;

[0010] A linkage scraping component, which is detachably mounted on the culture dish, and comprises a vibration section and a scraping section connected to the vibration section, wherein the vibration section is located below the cam, and when the cam rotates, the vibration section can be triggered to operate, so as to drive the scraping section to scrape the inner wall of the culture dish; and a built-in scraping component, which is detachably mounted in the culture dish, and is located below the vibration section of the linkage scraping component. When in the initial position, the built-in scraping component conflicts with the vibration section of the linkage scraping component, and can be triggered when the vibration section of the linkage scraping component is operating, so as to scrape the inner bottom wall of the culture dish.

[0011] As a further improvement of the present invention, the stirring and mixing member comprises a top plate, which is detachably mounted on the bottom of the sleeve plate, and a stirring shaft is detachably arranged therein, and the stirring shaft can penetrate into the bearing cavity of the culture dish;

[0012] A driven wheel is detachably mounted on the stirring shaft and meshes with the driving wheel;

[0013] A Jiaolong blade is detachably arranged at the rear end of the stirring shaft and can be inserted into the bearing cavity of the culture dish; and a plurality of groups of cross bars are detachably arranged on the stirring shaft, a first inclined blade is detachably installed at one end of each group of the cross bars, and a second inclined blade is detachably installed at the other end of each group of the cross bars, and the inclination directions of the first inclined blade and the second inclined blade are opposite.

[0014] As a further improvement of the present invention, the liquid mixing component includes a left vertical plate, which is detachably mounted on the culture dish;

[0015] A left connecting housing is detachably mounted on the left vertical plate, and a left stabilizing rod is detachably arranged inside the housing;

[0016] A left-hand screw rod is rotatably arranged in the left connecting housing, one end of which can pass through the left connecting housing, and a driving gear sleeve is detachably arranged on the end of the left-hand screw rod passing through the left connecting housing;

[0017] A linkage toothed disc, which is detachably mounted on the stirring shaft and meshes with the driving gear sleeve;

[0018] A left-handed ball bearing base is slidably arranged in the left connecting housing, is penetrated by the left stabilizing rod and the left-handed screw rod, and has a left-handed liquid mixing spray gun detachably arranged thereon;

[0019] a right vertical plate, which is detachably mounted on the culture dish;

[0020] A right connecting housing is detachably arranged on the right vertical plate, and a right stabilizing rod is detachably arranged inside the housing;

[0021] A right-hand screw rod is rotatably arranged in the right connecting housing, one end of which can pass through the right connecting housing, and the end of which passes through the right connecting housing passes through the driving gear sleeve;

[0022] A right-handed ball bearing base is slidably arranged in the right connecting housing and is penetrated by the right stabilizing rod and the right-handed screw rod; and a right mixed liquid spray gun is detachably mounted on the right-handed ball bearing base.

[0023] As a further improvement of the present invention, the cross-sectional circular area of ​​the linkage gear disc is four times the cross-sectional circular area of ​​the driving gear sleeve. In the initial state, the left-handed ball base is located on the left side of the left-connected housing, and the left-handed ball base is in contact with the inner wall of the left-connected housing. The right-handed ball base is located on the right side of the right-connected housing, and the right-handed ball base is in contact with the inner wall of the right-connected housing.

[0024] As a further improvement of the present invention, the vibration section of the linkage scraping member includes a connecting substrate, which is detachably mounted on the culture dish, and in which a straight rod is slidably arranged, and the straight rod can penetrate into the bearing cavity of the culture dish;

[0025] An upper roller, which is rotatably disposed on the upper section of the straight rod and is located below the cam;

[0026] An ear plate, which is integrally formed and arranged on the upper section of the straight rod, and on which a sliding rod is detachably arranged, and the sliding rod can slide in the connecting base plate;

[0027] A first spring, which is removably sleeved on the slide rod;

[0028] A limit block is detachably arranged at one end of the slide bar passing through the connecting base plate, and is fitted with the connecting base plate; and a lower roller is rotatably arranged at the lower section of the straight rod.

[0029] As a further improvement of the present invention, the first spring is located below the ear plate, and the first spring and the bottom surface of the ear plate are mutually fitted, and the first spring is located above the connecting substrate, and the first spring and the top surface of the connecting substrate are mutually fitted.

[0030] As a further improvement of the present invention, the size of the upper roller is the same as that of the lower roller, and the size of the upper roller is smaller than that of the cam.

[0031] As a further improvement of the present invention, the scraping section of the linkage scraping member includes a scraping frame, which is detachably mounted on the straight rod and surrounds the straight rod therein, and can penetrate into the bearing cavity of the culture dish;

[0032] A plurality of groups of first scraping brushes are detachably mounted on the four outer walls of the scraping machine frame, and the bristles of the plurality of groups of first scraping brushes are respectively in contact with the four inner walls of the culture dish; and a plurality of groups of first connecting grooves are respectively opened on the four outer walls of the scraping machine frame, and a group of first scraping brushes is provided between every two of the first connecting grooves.

[0033] As a further improvement of the present invention, the built-in scraping member includes a guide rod, which is located below the scraping machine frame, one end of which is detachably arranged on an inner side wall of the culture dish, and the other end of which is detachably arranged with a connecting rod, and one end of the connecting rod is detachably arranged on another inner side wall opposite to the inner side wall of the culture dish;

[0034] A scraping plate is slidably arranged on the guide rod, and a connecting block is detachably arranged on the top surface of the scraping plate. The connecting block has an inclined surface, and when the scraping plate is located at the initial position, the highest point of the inclined surface contacts the lower roller;

[0035] A second spring is removably sleeved on the guide rod, and one end of the second spring is in contact with the scraper plate, and the other end of the second spring is in contact with the connecting rod;

[0036] A plurality of groups of second scraping brushes are detachably mounted on the bottom surface of the scraping plate, and the bristles of the plurality of groups of second scraping brushes are respectively in contact with the inner bottom wall of the culture dish; and a plurality of groups of second connecting grooves are all opened on the bottom surface of the scraping plate, and the plurality of groups of second connecting grooves can penetrate the scraping plate from bottom to top, and a group of second scraping brushes is arranged between every two of the second connecting grooves.

[0037] As a further improvement of the present invention, the cross-sectional circular area of ​​the connecting rod is larger than the cross-sectional circular area of ​​the guide rod, the caliber of the second spring is smaller than the caliber of the connecting rod, a first magnetic adsorption pad is provided on one side surface of the guide rod, and a second magnetic adsorption pad is provided on one side surface of the connecting rod. The first magnetic adsorption pad is adsorbed to an inner wall of the culture dish, and the second magnetic adsorption pad is adsorbed to the other inner wall opposite to the inner wall of the culture dish.

[0038] In general, compared with the prior art, the above technical solution conceived by the present invention has the following beneficial effects:

[0039] 1. The cell culture dish of the present invention can simultaneously trigger the stirring and mixing component to stir the mixed liquid and the linked scraping component to move up and down and scrape the liquid through the arranged driving component. After the driving motor drives the driving rod to rotate, the arranged stirring and mixing component drives the driving wheel to rotate, so that the driving wheel drives the driven wheel to rotate. After the driven wheel rotates, the driven wheel drives the dragon leaf to rotate, so as to mix and stir the aqueous solution and the cleaning liquid in the carrying cavity of the culture dish. In addition, during the mixing and stirring process, the mixed solution of the aqueous solution and the cleaning liquid can be driven to shake to enhance the flushing effect of the inner bottom wall and the inner side wall of the culture dish. The arranged first inclined leaf and the second inclined leaf can also mix and stir in the inclined direction to further increase the mixing and stirring effect of the aqueous solution and the cleaning liquid.

[0040] 2. The cell culture dish of the present invention has a liquid mixing component arranged therein. After the driving component as a whole drives the stirring and mixing component to rotate, the stirring shaft rotates. Since the linkage gear disc is sleeved on the stirring shaft, the linkage gear disc can be triggered and started at the same time. After the linkage gear disc rotates, the driving gear sleeve as a whole rotates to drive the left-hand screw and the right-hand screw to rotate. Since the running directions of the left-hand screw and the right-hand screw are opposite, after the left-hand screw and the right-hand screw rotate at the same time, the left liquid mixing spray gun and the right liquid mixing spray gun move closer to or away from each other. The left liquid mixing spray gun and the right liquid mixing spray gun are turned on to spray the cleaning liquid into the culture dish along a straight line. This can avoid the problem of single-point release of the cleaning liquid so that the cleaning liquid takes a long time to mix with the aqueous solution. At the same time, the mixing of the cleaning liquid and the aqueous solution can be promoted when the cleaning liquid is stirred by the stirring and mixing component.

[0041] 3. The cell culture dish of the present invention is provided with a linkage scraping member. When the cam rotates, the protruding section of the cam touches the upper roller and can push the upper roller to move downward as a whole, so that the vibrating section of the linkage scraping member and the cam as a whole are linked to drive the straight rod to move downward. Since the straight rod is connected to the scraping machine frame and can make the scraping machine frame move downward as a whole, the first scraping brush and the four inner walls of the culture dish are scraped to scrape off the impurities on the four inner walls of the culture dish. Since the straight rod and the ear plate move downward, the first spring can be compressed. After the protruding section of the cam is separated from the upper roller, the first spring loses compression and rebounds to drive the scraping machine frame to move upward as a whole, so as to continuously scrape the four inner walls of the culture dish. In the process of scraping up and down of the scraping machine frame, the cleaning liquid and the aqueous solution in the culture dish can be shaken to further accelerate the mixing of the cleaning liquid and the aqueous solution.

[0042] 4. The cell culture dish of the present invention can drive the lower roller to move downward after the straight rod moves downward by means of the built-in scraping member. Since the lower roller initially contacts the highest point of the inclined plane, the lower roller moves from the highest point of the inclined plane toward the lowest point after the lower roller moves downward, so that the scraping plate moves right as a whole, thereby driving the second scraping brush to scrape and remove impurities from the inner bottom wall of the culture dish. Since the rightward movement of the scraping plate can compress the second spring, after the lower roller retreats, the second spring rebounds to move the scraping plate to the left to scrape the inner bottom wall of the culture dish again. After the second spring continuously moves rightward and leftward, the cleaning liquid and the aqueous solution in the culture dish can be further mixed to promote its scraping operation on the inner bottom wall of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a schematic diagram of the overall structure of the cell culture dish of the present invention;

[0044] Figure 2 It is a schematic diagram of the structure of the cell culture dish of the present invention when viewed from another angle;

[0045] Figure 3 An exploded view of the cell culture dish of the present invention;

[0046] Figure 4 It is a front view of the cell culture dish of the present invention;

[0047] Figure 5 It is a structural schematic diagram of the invention when the linked scraping component and the built-in scraping component conflict with each other;

[0048] Figure 6 It is a schematic diagram of the overall structure of the built-in scraping component of the present invention;

[0049] Figure 7It is a schematic diagram of the overall structure of the linkage scraping component of the present invention;

[0050] Figure 8 It is a partial structural schematic diagram of the linkage scraping component of the present invention;

[0051] Fig. 9 It is a schematic diagram of the overall structure of the liquid mixing component of the present invention;

[0052] Fig.10 It is a schematic diagram of the structure when the driving component and the stirring and mixing component of the present invention are combined with each other;

[0053] Fig.11 It is a schematic structural diagram of the present invention when the driving component and the stirring and mixing component are combined with each other from another angle;

[0054] Fig.12 It is a schematic diagram of the overall structure of the stirring and mixing component of the present invention;

[0055] Fig.13 For the present invention Figure 6 A magnified image of point A;

[0056] Fig.14 For the present invention Figure 7 Enlarged view of point B.

[0057] In all the drawings, the same reference numerals represent the same technical features, specifically: 1, culture dish; 2, driving member; 21, connecting frame; 22, sleeve disc; 23, driving motor; 24, driving rod; 25, first bevel gear; 26, connecting seat; 27, linkage rod; 28, second bevel gear; 281, cam; 29, driving wheel; 3, stirring and mixing member; 31, top plate; 32, stirring shaft; 33, driven wheel; 34, dragon blade; 35, cross bar; 36, first inclined blade; 37, second inclined blade; 4, liquid mixing member; 41, left vertical plate; 42, left connecting housing; 43, left stabilizing rod; 44, left-handed screw; 45, linkage gear disc; 46, driving gear sleeve; 47, Left-handed ball bearing base; 48, left mixed liquid spray gun; 49, right vertical plate; 491, right connecting housing; 492, right stabilizing rod; 493, right-handed screw rod; 494, right-handed ball bearing base; 495, right mixed liquid spray gun; 5, linked scraping member; 51, connecting base plate; 52, straight rod; 53, upper roller; 54, ear plate; 55, slide bar; 56, first spring; 57, limit block; 58, lower roller; 59, scraping machine frame; 591, first scraping brush; 592, first connecting groove; 6, built-in scraping member; 61, guide rod; 62, connecting rod; 63, scraping plate; 64, second spring; 65, second scraping brush; 66, second connecting groove; 67, connecting block; 68, inclined plane. DETAILED DESCRIPTION

[0058] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] The terms used herein are only for describing specific embodiments and are not intended to limit the present invention. The terms "comprise", "include", etc. used herein indicate the existence of the features, steps, operations and / or components, but do not exclude the existence or addition of one or more other features, steps, operations or components.

[0060] All terms (including technical and scientific terms) used herein have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used herein should be interpreted as having a meaning consistent with the context of this specification, and should not be interpreted in an idealized or overly rigid manner.

[0061] In the embodiment, Figure 1-14 A cell culture dish is provided, wherein Figure 1 It is a schematic diagram of the overall structure of the cell culture dish of the present invention; Figure 2 It is a schematic diagram of the structure of the cell culture dish of the present invention when viewed from another angle; Figure 3 An exploded view of the cell culture dish of the present invention; Figure 4 It is a front view of the cell culture dish of the present invention; Figure 5 It is a structural schematic diagram of the invention when the linked scraping component and the built-in scraping component conflict with each other; Figure 6 It is a schematic diagram of the overall structure of the built-in scraping component of the present invention; Figure 7 It is a schematic diagram of the overall structure of the linkage scraping component of the present invention; Figure 8 It is a partial structural schematic diagram of the linkage scraping component of the present invention; Fig. 9 It is a schematic diagram of the overall structure of the liquid mixing component of the present invention; Fig.10 It is a schematic diagram of the structure when the driving component and the stirring and mixing component of the present invention are combined with each other; Fig.11 It is a schematic structural diagram of the present invention when the driving component and the stirring and mixing component are combined with each other from another angle; Fig.12 It is a schematic diagram of the overall structure of the stirring and mixing component of the present invention; Fig.13 For the present invention Figure 6 A magnified image of point A; Fig.14 For the present invention Figure 7The enlarged view at B of the figure shows that it includes a culture dish 1 having a bearing cavity therein; a driving member 2 including a connecting frame 21 which is detachably mounted on the culture dish 1, on which a set of discs 22 is detachably arranged, on which a driving motor 23 is arranged, and the output end of the driving motor 23 can be inserted into the set of discs 22, on which a driving rod 24 is detachably arranged at the output end of the driving motor 23, on which a first bevel gear 25 and a driving wheel 29 are removably arranged in sequence; a connecting seat 26 which is detachably mounted on the set of discs 22, in which a linkage rod 27 is rotatably arranged, on one end of the linkage rod 27 a second bevel gear 28 meshing with the first bevel gear 25 is detachably arranged, and on the other end of the linkage rod 27 a cam 281 is detachably arranged; a stirring and mixing member 3 which is detachably arranged on the set of discs 22 and meshes with the driving wheel 29, and the stirring and mixing member 3 ... It can penetrate into the bearing cavity of the culture dish 1; a liquid mixing member 4, which is detachably installed on the culture dish 1, and can move with the stirring and mixing member 3 when the stirring and mixing member 3 moves, and is used to feed liquid into the bearing cavity; a linkage scraping member 5, which is detachably installed on the culture dish 1, and is composed of a vibration section and a scraping section connected to the vibration section, and the vibration section is located below the cam 281, and when the cam 281 rotates, it can trigger the vibration section to drive the scraping section to scrape the inner wall of the culture dish 1; and a built-in scraping member 6, which is detachably installed in the culture dish 1, and is located below the vibration section of the linkage scraping member 5. When it is in the initial position, it conflicts with the vibration section of the linkage scraping member 5, and can be triggered when the vibration section of the linkage scraping member 5 is working, so as to scrape the inner bottom wall of the culture dish 1.

[0062] First, the overall operating principle of the driving component 2 is further explained. After the staff turns on the driving motor 23, the driving rod 24 can be driven to rotate, and the first bevel gear 25 on the driving rod 24 can be driven to rotate. After the first bevel gear 25 rotates, the second bevel gear 28 can be driven to rotate, so as to change the vertical rotation into the horizontal rotation, so as to drive the cam 281 to rotate. After the cam 281 rotates, when the protruding section of the cam 281 touches the upper roller 53, the upper roller 53 can be pushed to move downward as a whole. When the semicircular section of the cam 281 touches 83, it does not interact with the upper roller 53. At the same time, when the driving rod 24 rotates, it can synchronously drive the driving wheel 29 to rotate, so that the driving wheel 29 drives the driven wheel 33 to rotate, so that the stirring and mixing component 3 is linked as a whole.

[0063] Next, the stirring and mixing component 3 as a whole is given a more specific structure and construction for further explanation. The stirring and mixing component 3 includes a top plate 31, which is detachably mounted on the bottom of the sleeve plate 22, and a stirring shaft 32 is detachably arranged therein, and the stirring shaft 32 can penetrate into the bearing cavity of the culture dish 1; a driven wheel 33, which is detachably mounted on the stirring shaft 32 and meshes with the driving wheel 29; a dragon blade 34, which is detachably arranged at the tail end of the stirring shaft 32 and can penetrate into the bearing cavity of the culture dish 1; and a plurality of groups of cross bars 35, which are detachably arranged on the stirring shaft 32, and a first inclined blade 36 is detachably mounted on one end of each group of cross bars 35, and a second inclined blade 37 is detachably mounted on the other end of each group of cross bars 35, and the inclination directions of the first inclined blade 36 and the second inclined blade 37 are opposite;

[0064] Next, the overall use principle of the stirring and mixing component 3 is further explained. After the driving motor 23 drives the driving rod 24 to rotate, it drives the active wheel 29 to rotate, so that the active wheel 29 drives the driven wheel 33 to rotate. After the driven wheel 33 rotates, the driven wheel 33 drives the dragon blade 34 to rotate, so as to mix and stir the aqueous solution and the cleaning liquid in the carrying cavity of the culture dish 1. At the same time, during the mixing and stirring process, the mixed solution of the aqueous solution and the cleaning liquid can be driven to shake to enhance the flushing effect of the inner bottom wall and the inner side wall of the culture dish 1. The first inclined blade 36 and the second inclined blade 37 can be arranged to mix and stir in an inclined direction to further increase the mixing and stirring effect of the aqueous solution and the cleaning liquid.

[0065] Next, the liquid mixing component 4 is given a more specific structure and construction as a whole for further explanation. The liquid mixing component 4 includes a left vertical plate 41, which is detachably mounted on the culture dish 1; a left connecting housing 42, which is detachably mounted on the left vertical plate 41, and a left stabilizing rod 43 is detachably arranged therein; a left-handed screw rod 44, which is rotatably arranged in the left connecting housing 42, one end of which can pass through the left connecting housing 42, and a driving gear sleeve 46 is detachably arranged on the end of the left-handed screw rod 44 passing through the left connecting housing 42; a linkage gear disc 45, which is detachably mounted on the stirring shaft 32 and meshes with the driving gear sleeve 46; a left-handed ball bearing base 47, which is slidably arranged in the left connecting housing 42, and its It is penetrated by the left stabilizing rod 43 and the left-hand screw 44, on which a left liquid mixing spray gun 48 is detachably arranged; the right vertical plate 49, which is detachably mounted on the culture dish 1; the right connecting housing 491, which is detachably arranged on the right vertical plate 49, in which a right stabilizing rod 492 is detachably arranged; the right-hand screw 493, which is rotatably arranged in the right connecting housing 491, one end of which can pass through the right connecting housing 491, and the end of which passes through the right connecting housing 491 is inserted into the driving gear sleeve 46; the right-hand ball base 494, which is slidably arranged in the right connecting housing 491, and is penetrated by the right stabilizing rod 492 and the right-hand screw 493; and the right liquid mixing spray gun 495, which is detachably mounted on the right-hand ball base 494;

[0066] Next, the overall use principle of the liquid mixing component 4 is further explained. After the driving component 2 drives the stirring and mixing component 3 to rotate as a whole, the stirring shaft 32 is rotated. Since the linkage gear plate 45 is sleeved on the stirring shaft 32, the linkage gear plate 45 can be triggered and started at the same time. After the linkage gear plate 45 rotates, the driving gear sleeve 46 is driven to rotate as a whole, so as to drive the left-hand screw 44 and the right-hand screw 493 to rotate. Since the left-hand screw 44 and the right-hand screw 493 run in opposite directions, When the left-hand screw 44 and the right-hand screw 493 rotate at the same time, the left mixing liquid spray gun 48 and the right mixing liquid spray gun 495 move closer to or away from each other. The left mixing liquid spray gun 48 and the right mixing liquid spray gun 495 are turned on to spray the cleaning liquid into the culture dish 1 in a straight line. This can avoid the problem of single-point release of the cleaning liquid so that the cleaning liquid takes a long time to mix with the aqueous solution. At the same time, it can promote the mixing of the cleaning liquid and the aqueous solution when stirred by the stirring and mixing component 3.

[0067] In some embodiments, in order to enable the stroke of the linkage gear plate 45 after one circle of movement to be greater than the stroke of the driving gear sleeve 46 after one circle of movement, and to enable the left-handed ball base 47 and the right-handed ball base 494 to move left and right at the beginning of the movement, the cross-sectional circular area of ​​the linkage gear plate 45 is four times the cross-sectional circular area of ​​the driving gear sleeve 46. In the initial state, the left-handed ball base 47 is located on the left side of the left connecting housing 42, and the left-handed ball base 47 is in contact with the inner wall of the left connecting housing 42. The right-handed ball base 494 is located on the right side of the right connecting housing 491, and the right-handed ball base 494 is in contact with the inner wall of the right connecting housing 491.

[0068] Next, the vibration section of the linkage scraping member 5 is given a more specific structure and construction for further explanation. The vibration section of the linkage scraping member 5 includes a connecting substrate 51, which is detachably mounted on the culture dish 1, and in which a straight rod 52 is slidably arranged, and the straight rod 52 can penetrate into the bearing cavity of the culture dish 1; an upper roller 53, which is rotatably arranged on the upper section of the straight rod 52 and is located below the cam 281; an ear plate 54, which is integrally formed and arranged on the upper section of the straight rod 52, and on which a sliding rod 55 is detachably arranged, and the sliding rod 55 can slide in the connecting substrate 51; a first spring 56, which is removably sleeved on the sliding rod 55; a limiting block 57, which is detachably arranged on one end of the sliding rod 55 passing through the connecting substrate 51, and is in contact with the connecting substrate 51; and a lower roller 58, which is rotatably arranged on the lower section of the straight rod 52;

[0069] Next, the use principle of the vibration section of the linkage scraping member 5 is further explained. When the cam 281 rotates, the protruding section of the cam 281 touches the upper roller 53, which can push the upper roller 53 to move downward as a whole, so that the vibration section of the linkage scraping member 5 and the cam 281 are linked as a whole to drive the straight rod 52 to move downward. Since the straight rod 52 is connected to the scraping machine frame 59 and can make the scraping machine frame 59 move downward as a whole, the first scraping brush 591 scrapes the four inner side walls of the culture dish 1. The scraper frame 59 is adapted to scrape off the impurities on the four inner walls of the culture dish 1. As the straight rod 52 and the ear plate 54 move downward, the first spring 56 can be compressed. After the protruding section of the cam 281 is separated from the upper roller 53, the first spring 56 loses compression and rebounds to drive the scraper frame 59 to move upward as a whole, so as to continuously scrape the four inner walls of the culture dish 1. In the process of the scraper frame 59 scraping up and down, the cleaning liquid and the aqueous solution in the culture dish 1 can be vibrated to further accelerate the mixing of the cleaning liquid and the aqueous solution.

[0070] In some embodiments, more specifically, in order to enhance the stability of the first spring 56 during operation, the first spring 56 is located below the ear plate 54, and the first spring 56 and the bottom surface of the ear plate 54 are in contact with each other, and the first spring 56 is located above the connecting substrate 51, and the first spring 56 and the top surface of the connecting substrate 51 are in contact with each other.

[0071] In some embodiments, more specifically, in order to enable the cam 281 to continuously touch the upper roller 53 , the size of the upper roller 53 is the same as that of the lower roller 58 , and the size of the upper roller 53 is smaller than that of the cam 281 .

[0072] Next, a more specific structure and construction is given to the scraping section of the linkage scraping member 5 for further explanation. The scraping section of the linkage scraping member 5 includes a scraping machine frame 59, which is detachably mounted on the straight rod 52 and surrounds the straight rod 52 therein, and can penetrate into the bearing cavity of the culture dish 1; multiple groups of first scraping brushes 591, which are detachably mounted on the four outer walls of the scraping machine frame 59, and the bristles of the multiple groups of first scraping brushes 591 are respectively in conflict with the four inner walls of the culture dish 1; and multiple groups of first connecting grooves 592, which are respectively opened on the four outer walls of the scraping machine frame 59, and a group of first scraping brushes 591 is provided between every two first connecting grooves 592;

[0073] Next, the scraping section of the linked scraping component 5 is further explained. Since the bristles of the first scraping brush 591 on the four outer walls of the scraper frame 59 conflict with the four inner walls of the culture dish 1, the scraper frame 59 can strengthen the scraping operation of the first scraping brush 591 on the four inner walls of the culture dish 1 when moving up and down. By opening multiple groups of first connecting grooves 592, the inner wall surface of the scraper frame 59 and its outer wall surface can be connected to each other when mixing liquids.

[0074] Next, the scraping section of the built-in scraping member 6 is given a more specific structure and construction for further explanation. The built-in scraping member 6 includes a guide rod 61, which is located below the scraper frame 59, one end of which is detachably arranged on an inner wall of the culture dish 1, and the other end of which is detachably arranged with a connecting rod 62, and one end of the connecting rod 62 is detachably arranged on the other inner wall opposite to the inner wall of the culture dish 1; a scraping plate 63, which is slidably arranged on the guide rod 61, and a connecting block 67 is detachably arranged on the top surface thereof, and a slope 68 is provided on the connecting block 67, which is located in the initial position When the scraper 63 is removed, the highest point of the inclined surface 68 contacts the lower roller 58; the second spring 64 is removably sleeved on the guide rod 61, and one end of the second spring 64 contacts the scraper plate 63, and the other end of the second spring 64 contacts the connecting rod 62; multiple groups of second scraping brushes 65 are detachably mounted on the bottom surface of the scraper plate 63, and the bristles of the multiple groups of second scraping brushes 65 contact the inner bottom wall of the culture dish 1 respectively; and multiple groups of second connecting grooves 66 are all opened on the bottom surface of the scraper plate 63, and the multiple groups of second connecting grooves 66 can penetrate the scraper plate 63 from bottom to top, and there is a group of second scraping brushes 65 between every two second connecting grooves 66;

[0075] Next, the overall use principle of the built-in scraping component 6 is further explained. When the straight rod 52 moves downward, it can drive the lower roller 58 to move downward. Since the lower roller 58 initially conflicts with the highest point of the inclined surface 68, the lower roller 58 moves from the highest point of the inclined surface 68 to the lowest point after moving downward, so that the scraping plate 63 moves right as a whole, so as to drive the second scraping brush 65 to scrape and remove impurities on the inner bottom wall of the culture dish 1. Since the rightward movement of the scraping plate 63 can compress the second spring 64, after the lower roller 58 retreats, the second spring 64 rebounds to move the scraping plate 63 to the left to scrape the inner bottom wall of the culture dish 1 again. After the second spring 64 continues to move right and left, it can further mix the cleaning liquid and the aqueous solution in the culture dish 1 to promote its scraping operation on the inner bottom wall of the culture dish 1.

[0076] In some embodiments, in order to further facilitate the overall installation of the built-in scraping member 6 on the inner wall of the culture dish 1, the cross-sectional circular area of ​​the connecting rod 62 is larger than the cross-sectional circular area of ​​the guide rod 61, the diameter of the second spring 64 is smaller than the diameter of the connecting rod 62, and a first magnetic adsorption pad is provided on one side of the guide rod 61, and a second magnetic adsorption pad is provided on one side of the connecting rod 62. The first magnetic adsorption pad is adsorbed to an inner wall of the culture dish 1, and the second magnetic adsorption pad is adsorbed to the other inner wall opposite to the inner wall of the culture dish 1. It should also be noted that there is a magnetic sheet with opposite magnetic properties to the guide rod 61 and the connecting rod 62 on the inner wall of the culture dish 1, and the guide rod 61 and the connecting rod 62 are attracted to the inner wall of the culture dish 1 through the principle of opposite attraction.

[0077] In summary, the driving member 2 is provided to simultaneously trigger the stirring and mixing member 3 to stir the mixed liquid and the linked scraping member 5 to perform the upward and downward moving scraping operation. The stirring and mixing member 3 is arranged, and after the driving motor 23 drives the driving rod 24 to rotate, the driving wheel 29 is driven to rotate, so that the driving wheel 29 drives the driven wheel 33 to rotate. After the driven wheel 33 rotates, the driven wheel 33 drives the dragon blade 34 to rotate, so as to mix and stir the aqueous solution and the cleaning liquid in the carrying cavity of the culture dish 1, and can simultaneously drive the mixed solution of the aqueous solution and the cleaning liquid to shake in the process of mixing and stirring to enhance the flushing effect of the inner bottom wall and the inner side wall of the culture dish 1, and through the first inclined blade 3 arranged 6 and the second inclined blade 37 can also perform mixing and stirring in an inclined direction to further increase the mixing and stirring effect on the aqueous solution and the cleaning liquid. Through the arranged mixing component 4, after the driving component 2 drives the mixing and stirring component 3 to rotate as a whole, the stirring shaft 32 is rotated. Since the linkage gear disc 45 is sleeved on the stirring shaft 32, the linkage gear disc 45 can be triggered and started at the same time. After the linkage gear disc 45 rotates, the driving gear sleeve 46 is driven to rotate as a whole to drive the left-hand screw 44 and the right-hand screw 493 to rotate. Since the left-hand screw 44 and the right-hand screw 493 run in opposite directions, when the left-hand screw 44 and the right-hand screw 493 rotate at the same time, the left mixing liquid spray gun 48 and the right mixing liquid spray gun 495 are moved closer to or farther away from each other, and the left mixing liquid spray gun 48 and the right mixing liquid spray gun 495 are turned on so that the left mixing liquid spray gun 48 and the right mixing liquid spray gun 495 spray the cleaning liquid into the culture dish 1 along a straight line. This can avoid the problem of single-point release of the cleaning liquid so that the cleaning liquid takes a long time to mix with the aqueous solution. At the same time, when the stirring and mixing component 3 is stirred, the mixing of the cleaning liquid and the aqueous solution can be promoted. Through the arranged linkage scraping component 5, when the cam 281 rotates, the protruding section of the cam 281 touches the upper roller 53, which can push the upper roller 53 to move downward as a whole, so that the vibration section of the linkage scraping component 5 is linked with the cam 281 as a whole, so as to drive the straight rod 52 to move downward. Since the straight rod 52 is connected to the scraping component 5 The scraper frame 59 is connected to the upper roller 53, and the scraper frame 59 can be moved downward as a whole, so that the first scraping brush 591 and the four inner walls of the culture dish 1 can perform a scraping operation to scrape off the impurities on the four inner walls of the culture dish 1. As the straight rod 52 and the ear plate 54 move downward, the first spring 56 can be compressed. After the protruding section of the cam 281 is separated from the upper roller 53, the first spring 56 loses compression and rebounds to drive the scraper frame 59 to move upward as a whole, so as to continue to scrape the four inner walls of the culture dish 1. In the process of scraping up and down of the scraper frame 59, the cleaning liquid and the aqueous solution in the culture dish 1 can be vibrated to further accelerate the mixing of the cleaning liquid and the aqueous solution. By arranging the built-in scraping member 6,When the straight rod 52 moves downward, it can drive the lower roller 58 to move downward. Since the lower roller 58 initially contacts the highest point of the inclined surface 68, the lower roller 58 moves from the highest point of the inclined surface 68 to the lowest point after the lower roller 58 moves downward, so that the scraping plate 63 moves rightward as a whole, so as to drive the second scraping brush 65 to scrape and remove impurities from the inner bottom wall of the culture dish 1. Since the scraping plate 63 moves rightward, the second spring 64 can be compressed. After the lower roller 58 is retracted, the second spring 64 rebounds to move the scraping plate 63 to the left to scrape the inner bottom wall of the culture dish 1 again. After the second spring 64 continues to move rightward and leftward, it can further mix the cleaning liquid and the aqueous solution in the culture dish 1 to promote its scraping operation on the inner bottom wall of the culture dish 1.

[0078] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture dish, characterized in that: It includes A culture dish (1) having a load-bearing cavity therein; A driving member (2), comprising a connecting frame (21) which is detachably mounted on the culture dish (1), on which a set of discs (22) is detachably arranged, on which a driving motor (23) is arranged, and the output end of the driving motor (23) can be inserted into the set of discs (22), on which a driving rod (24) is detachably arranged on the output end of the driving motor (23), and on which a first bevel gear (25) and a driving wheel (29) are removably arranged in sequence; A connecting seat (26) is detachably mounted on the sleeve (22), a linkage rod (27) is rotatably arranged therein, a second bevel gear (28) meshing with the first bevel gear (25) is detachably arranged on one end of the linkage rod (27), and a cam (281) is detachably arranged on the other end of the linkage rod (27); A stirring and mixing member (3) which is detachably arranged on the sleeve disc (22) and meshes with the driving wheel (29) so as to be able to penetrate into the bearing cavity of the culture dish (1); A liquid mixing component (4) which is detachably mounted on the culture dish (1) and can move along with the stirring and mixing component (3) when the stirring and mixing component (3) moves, and is used to perform a liquid feeding operation in the bearing cavity; A linkage scraping component (5) is detachably mounted on the culture dish (1), and comprises a vibrating section and a scraping section connected to the vibrating section, wherein the vibrating section is located below the cam (281), and when the cam (281) rotates, the vibrating section can be triggered to operate, thereby driving the scraping section to scrape the inner wall of the culture dish (1); as well as A built-in scraping member (6) is detachably mounted in the culture dish (1), and is located below the vibration section of the linked scraping member (5). In an initial position, the built-in scraping member (6) is in conflict with the vibration section of the linked scraping member (5), and can be triggered when the vibration section of the linked scraping member (5) is in operation, so as to scrape the inner bottom wall of the culture dish (1); The stirring and mixing component (3) comprises A top plate (31) is detachably mounted on the bottom of the sleeve plate (22), and a stirring shaft (32) is detachably arranged inside the top plate, and the stirring shaft (32) can penetrate into the bearing cavity of the culture dish (1); A driven wheel (33) is detachably mounted on the stirring shaft (32) and meshes with the driving wheel (29); A Jiaolong blade (34) is detachably arranged at the rear end of the stirring shaft (32) and is capable of penetrating into the bearing cavity of the culture dish (1); and A plurality of groups of cross bars (35) are detachably arranged on the stirring shaft (32); a first inclined blade (36) is detachably mounted on one end of each group of cross bars (35); a second inclined blade (37) is detachably mounted on the other end of each group of cross bars (35); and the first inclined blade (36) and the second inclined blade (37) are inclined in opposite directions; The liquid mixing component (4) comprises A left vertical plate (41) detachably mounted on the culture dish (1); A left connecting housing (42) is detachably mounted on the left vertical plate (41), and a left stabilizing rod (43) is detachably arranged inside the housing; A left-hand screw rod (44) is rotatably disposed in the left connecting housing (42), one end of which can pass through the left connecting housing (42), and a driving gear sleeve (46) is detachably disposed on the end of the left-hand screw rod (44) passing through the left connecting housing (42); A linkage toothed disc (45) is detachably mounted on the stirring shaft (32) and meshes with the driving gear sleeve (46); A left-handed ball bearing base (47) is slidably disposed in the left connecting housing (42), is penetrated by the left stabilizing rod (43) and the left-handed screw rod (44), and has a left-handed liquid mixing spray gun (48) detachably disposed thereon; A right vertical plate (49) detachably mounted on the culture dish (1); A right connecting housing (491) is detachably arranged on the right vertical plate (49), and a right stabilizing rod (492) is detachably arranged inside the housing; a right-hand screw rod (493) rotatably disposed in the right connecting housing (491), one end of which can pass through the right connecting housing (491), and the end of which passes through the right connecting housing (491) passes through the driving gear sleeve (46); a right-handed ball bearing base (494) slidably disposed in the right connecting housing (491) and penetrated by the right stabilizing rod (492) and the right-handed screw rod (493); and A right liquid mixing spray gun (495) is detachably mounted on the right-handed ball bearing base (494); The vibration section of the linked scraping component (5) comprises A connecting base plate (51) which is detachably mounted on the culture dish (1), and in which a straight rod (52) is slidably arranged, and the straight rod (52) is capable of penetrating into a bearing cavity of the culture dish (1); An upper roller (53) rotatably disposed on the upper section of the straight rod (52) and located below the cam (281); An ear plate (54) is integrally formed and arranged on the upper section of the straight rod (52), and a sliding rod (55) is detachably arranged on the ear plate, and the sliding rod (55) is capable of sliding in the connecting base plate (51); A first spring (56) which is removably sleeved on the slide rod (55); a limit block (57) which is detachably arranged on one end of the slide bar (55) passing through the connection base plate (51), and is in contact with the connection base plate (51); and A lower roller (58) rotatably disposed on the lower section of the straight rod (52); The scraping section of the linked scraping component (5) comprises A scraper frame (59) which is detachably mounted on the straight rod (52) and surrounds the straight rod (52) therein and is capable of penetrating into the bearing cavity of the culture dish (1); A plurality of groups of first scraping brushes (591) are detachably mounted on four outer side walls of the scraping machine frame (59), and the bristles of the plurality of groups of first scraping brushes (591) are respectively in contact with the four inner side walls of the culture dish (1); as well as A plurality of groups of first connecting grooves (592) are respectively provided on four outer side walls of the scraper frame (59), and a group of first scraping brushes (591) is provided between every two of the first connecting grooves (592); The built-in scraping member (6) comprises A guide rod (61) located below the scraper frame (59), one end of which is detachably arranged on an inner wall of the culture dish (1), and the other end of which is detachably arranged on a connecting rod (62), and one end of which is detachably arranged on another inner wall of the culture dish (1) opposite to the inner wall of the culture dish (1); A scraping plate (63) is slidably arranged on the guide rod (61), and a connecting block (67) is detachably arranged on the top surface of the scraping plate. The connecting block (67) has an inclined surface (68). When the scraping plate (63) is located at an initial position, the highest point of the inclined surface (68) contacts the lower roller (58). A second spring (64) which is removably sleeved on the guide rod (61), with one end of the second spring being in contact with the scraper plate (63) and the other end of the second spring being in contact with the connecting rod (62); A plurality of sets of second scraping brushes (65) are detachably mounted on the bottom surface of the scraping plate (63), and the bristles of the plurality of sets of second scraping brushes (65) are respectively in contact with the inner bottom wall of the culture dish (1); and A plurality of groups of second connecting grooves (66) are all formed on the bottom surface of the scraping plate (63), and the plurality of groups of second connecting grooves (66) can penetrate the scraping plate (63) from bottom to top, and a group of second scraping brushes (65) is provided between every two of the second connecting grooves (66).

2. The cell culture dish according to claim 1, characterized in that The cross-sectional circular area of ​​the linkage gear disc (45) is four times the cross-sectional circular area of ​​the driving gear sleeve (46). In an initial state, the left-handed ball base (47) is located on the left side of the left connection housing (42), and the left-handed ball base (47) fits against the inner wall of the left connection housing (42). The right-handed ball base (494) is located on the right side of the right connection housing (491), and the right-handed ball base (494) fits against the inner wall of the right connection housing (491).

3. The cell culture dish according to claim 2, characterized in that The first spring (56) is located below the ear plate (54), and the first spring (56) and the bottom surface of the ear plate (54) are in contact with each other. The first spring (56) is located above the connecting substrate (51), and the first spring (56) and the top surface of the connecting substrate (51) are in contact with each other.

4. The cell culture dish according to claim 3, characterized in that The size of the upper roller (53) is the same as the size of the lower roller (58), and the size of the upper roller (53) is smaller than the size of the cam (281).

5. The cell culture dish according to claim 4, characterized in that: The cross-sectional circular area of ​​the connecting rod (62) is larger than the cross-sectional circular area of ​​the guiding rod (61); the diameter of the second spring (64) is smaller than the diameter of the connecting rod (62); a first magnetic adsorption pad is provided on one side surface of the guiding rod (61); a second magnetic adsorption pad is provided on one side surface of the connecting rod (62); the first magnetic adsorption pad is adsorbed to an inner side wall of the culture dish (1); and the second magnetic adsorption pad is adsorbed to another inner side wall of the culture dish (1) opposite to the inner side wall.

Images