A complex probiotic capable of synergistically metabolizing pectic polysaccharides and application thereof

By co-culturing a compound probiotic strain of *Lactobacillus plantarum* and *Lactobacillus rhamnosus* in a pectin-based carbon source medium, the problem of low efficiency in pectin metabolism by a single strain was solved, achieving efficient metabolism of pectin polysaccharides and short-chain fatty acids, which can be applied in the food and pharmaceutical fields.

CN119286691BActive Publication Date: 2025-11-28SHIHEZI UNIVERSITY
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Patent Information

Application Number
CN202411390913.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-08
Publication Date
2025-11-28
Estimated Expiration
2044-10-08

AI Technical Summary

Technical Problem

Currently, probiotics are inefficient at metabolizing pectin polysaccharides, leading to interference with mineral absorption and reduced drug efficacy. Single strains also have limitations in terms of overall probiotic performance.

Method used

A compound probiotic strain consisting of Lactiplantibacillus plantarum FMBL L23180 CLL and Lacticaseibacillus rhamnosus FMBL L23121 YWT was co-cultured in a pectin-based carbon source medium to achieve rapid proliferation and efficient metabolism of pectin polysaccharides, producing a large amount of short-chain fatty acids.

Benefits of technology

Compound probiotics significantly increased biomass and prebiotic index in pectin-based carbon source culture medium, and the yield of short-chain fatty acids at the end of fermentation was significantly higher than that of single culture. It can be used to prepare products such as microcapsules, compound probiotic agents, fermented milk, fruit and vegetable beverages and probiotic milk tablets.

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Abstract

The present application relates to the field of biotechnology, in particular to a kind of complex probiotic bacteria capable of synergistic metabolism pectin polysaccharide and application thereof, the complex bacterial agent includes plant lactobacillus FMBL L23180CLL and rhamnose lactobacillus FMBL L23121YWT, preservation number is CCTCC NO:M 20241793 and CCTCC NO:M 20241794 respectively;Can be realized in the medium of pectin as carbon source only rapid proliferation, high-efficiency metabolism pectin polysaccharide, promote the growth of strain, biomass is significantly improved, and probiotic index is also significantly improved;In the process of co-culture, a large amount of short-chain fatty acid is produced, and the content of acetic acid and valeric acid produced at the end of fermentation is the highest, more than 90% of total fermentation product, can be used for preparing microcapsule, complex probiotic bacterial agent, fermented milk, fruit and vegetable beverage, plant milk, probiotic milk tablet and the like, and has wide application prospect.
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