Device and method for detecting antinuclear antibody by using immunofluorescence method

Abstract

The invention belongs to the technical field of medical detection technologies, particularly relates to a device and a method for performing antinuclear antibody detection by applying an immunofluorescence method, and aims to solve the problems that errors are easy to occur during detection due to the fact that a plurality of samples cannot be detected at the same time during actual use of an existing technical scheme, and the detection efficiency is low during use of the existing technical scheme. The device comprises an equipment box, a handle is fixedly mounted on the equipment box, a sheath fixedly sleeves the handle, and a mounting plate is fixedly mounted on one side of the handle. A plurality of specimen boxes and a plurality of detection mechanisms are arranged, so that a plurality of same test specimens can be detected; therefore, multiple groups of data can be obtained at the same time, transverse comparison is facilitated, the accuracy of data analysis is improved, meanwhile, stable connection with the wrist of an operator can be kept when the operator carries out handheld operation, the whole equipment can be prevented from falling off, and damage is avoided.

Description

Technical Field

[0001] The present invention relates to the field of medical detection technology, in particular to a device and method for performing antinuclear antibody detection using immunofluorescence method. Background Art

[0002] Fluorescence immunoassay technology is a commonly used technical means in biomedical testing. It has a series of advantages such as high sensitivity, strong specificity, fast detection speed, safety and stability. Fluorescence immunoassay technology can be used to measure very low levels of biologically active compounds, such as proteins (enzymes, receptors, antibodies), hormones (steroid compounds, thyroid hormones, phthalosteroid hormones), drugs and microorganisms. Currently, fluorescence immunoassay technology has been widely used in infectious disease detection, tumor marker detection, blood and cytology detection and other detection fields. According to the announcement number CN219162165U, a handheld immunofluorescence instrument is disclosed, which can achieve the advantages of simple structure, small size, easy to carry, convenient and fast card return, and time-saving and labor-saving. However, this technical solution still has the following shortcomings in actual use: 1. Currently, when testing fluorescent samples, only a single sample can be tested at a time, so it is impossible to test more than two samples simultaneously for parallel comparison. This may lead to errors in the test data when testing multiple identical samples. 2. Furthermore, when using a handheld detector to test samples, due to its relatively simple structure and the lack of corresponding devices to connect and fix the instrument to the arm, the detector may fall due to unstable holding and thus be damaged.

[0003] In response to the above problems, the present invention document proposes a device and method for antinuclear antibody testing using immunofluorescence. Summary of the Invention

[0004] The present invention provides a device and method for antinuclear antibody testing using the immunofluorescence method, which solves the shortcomings of existing technical solutions in that they cannot detect multiple samples simultaneously during actual use, making errors prone to occur during testing, and that existing technical solutions are prone to falling and damage during use.

[0005] The present invention provides the following technical solutions: A device for performing antinuclear antibody testing using an immunofluorescence method comprises an equipment box, a handle fixedly mounted on the equipment box, a protective sleeve fixedly mounted on the handle, a mounting plate fixedly mounted on one side of the handle, the mounting plate fixedly mounted on the top of the equipment box, two pads symmetrically fixedly mounted on the bottom of the equipment box, a support plate fixedly mounted inside the equipment box, an L-shaped cover plate slidably connected inside the equipment box, a seal fixedly mounted on one side of the L-shaped cover plate, the seal tightly fitting against the inner wall of the equipment box, one side of the L-shaped cover plate extending to the outside of the equipment box, the seal fixedly mounted on one side of the L-shaped cover plate, and the device further comprising: Multiple specimen supporting mechanisms are installed on the L-shaped cover at equal intervals, and are used to carry test specimens; Multiple detection mechanisms are installed at equal intervals on one side of the support plate, and the tops of the detection mechanisms extend above the equipment box and are installed on the top of the equipment box; A transmission mechanism, the transmission mechanism passes through the top inner wall of the equipment box and is connected to the top inner wall of the equipment box, and the bottom of the transmission mechanism is connected to the L-shaped cover plate; The fixing mechanism is installed on the handle and is used for tying with the arm of the staff.

[0006] In one possible design, the specimen support mechanism includes a specimen box fixedly mounted on an L-shaped cover plate, both sides of the specimen box are rotatably connected to an adjustment shaft, a plurality of L-shaped pressing plates are fixedly sleeved at equal intervals on the adjustment shaft, one side of the L-shaped pressing plate extends into the specimen box, and the L-shaped pressing plate is used to press the test specimen.

[0007] In a possible design, a rotating plate is fixedly installed at one end of the adjustment shaft, a mounting opening is opened on the rotating plate, an elastic clip is fixedly installed in the mounting opening, a card shaft is movably mounted in the elastic clip, and one end of the card shaft is fixedly installed on one side of the specimen box.

[0008] In one possible design, the detection mechanism includes a support frame fixedly mounted on one side of a support plate, a mounting cover fixedly mounted on the support frame, a camera fixedly mounted on the top inner wall of the mounting cover, a plurality of fill lights fixedly mounted at equal intervals on the bottom of the support frame, a transmission wire electrically connected to the camera, the top end of the transmission wire extends to the top of the equipment box and is electrically connected to an immunofluorescence instrument, and the immunofluorescence instrument is fixedly mounted on the top of the equipment box.

[0009] In one possible design, two limit plates are symmetrically fixedly installed on the bottom of the support plate, and two limit grooves are symmetrically opened on the L-shaped cover plate. The bottom of the limit plate extends into the corresponding limit groove and is slidably connected to the inner wall of the limit groove.

[0010] In one possible design, the transmission mechanism includes a rotating shaft that passes through the top inner wall of the equipment box and is rotatably connected to the top inner wall of the equipment box, a rotating wheel is fixedly installed on the top of the rotating shaft, a swing rod is fixedly installed on the bottom end of the rotating shaft, a stop shaft is fixedly installed on one side of the bottom of the swing rod, a transmission ring located on one side of the support plate is fixedly installed on the L-shaped cover plate, the bottom end of the stop shaft extends into the transmission ring, and the stop shaft and the transmission ring are matched for transmission.

[0011] In one possible design, a positioning ring is fixedly installed on the top of the equipment box, and the rotating shaft passes through the center point of the positioning ring. Two slots are symmetrically opened on the top of the positioning ring, and the angle between the two slots is 90°. A plug rod is slidingly connected through the rotating wheel, and the plug rod is movably engaged with the two slots respectively. A tension spring located above the rotating wheel is sleeved on the plug rod, and the top and bottom ends of the tension spring are fixedly connected to the top of the plug rod and the top of the rotating wheel respectively.

[0012] In one possible design, the fixing mechanism includes a mounting frame fixedly mounted on the handle, a connecting plate rotatably connected to the mounting frame, a strap fixedly mounted on one side of the connecting plate, the strap being used to be mounted on the wrist of the operator, a ring being mounted on the strap, a clamping assembly being mounted in the ring, and the top of the clamping assembly extending above the ring and connected to the top of the ring.

[0013] In one possible design, the clamping mechanism includes two clamping plates symmetrically and slidingly connected in the ring, and the two clamping plates are both used to clamp and position the strap, and an elastic plate is fixedly installed on the side of the two clamping plates close to each other, and the bottoms of the two elastic plates are fixedly installed on the bottom inner wall of the ring, and two transmission rods are symmetrically and slidingly connected on the top inner wall of the ring, and the bottom ends of the two transmission rods are fixedly installed with the same pressure rod, which is used to press the two elastic plates to cause elastic deformation of the two elastic plates, and the top ends of the two transmission rods are fixedly installed with the same transmission plate, and the top of the transmission plate is fixedly connected with a nut, and the top of the ring is rotatably connected with an adjusting screw, which passes through the transmission plate and the nut respectively and is threadedly connected to the nut.

[0014] A method for testing antinuclear antibodies using immunofluorescence, applied to the above-mentioned device for testing antinuclear antibodies using immunofluorescence, comprises the following steps: S1. Place the collected test samples in the corresponding specimen box in sequence, then flip the corresponding two rotating plates to drive the two adjustment shafts to rotate, thereby driving the corresponding multiple L-shaped pressing pieces to rotate, so that one side of the L-shaped pressing piece moves into the specimen box, pressing and positioning the test specimens to prevent the test specimens from shaking in the specimen box; S2. When the test specimen is pressed and positioned, the elastic clip can be clamped with the corresponding clamping shaft, so that the rotating plate can be positioned, thereby limiting the adjustment shaft and further maintaining the clamping force of the L-shaped pressing piece on the test specimen without change; S3. The rotating wheel is turned to drive the rotating shaft to rotate, which can drive the swing arm to rotate. When the swing arm rotates, it can drive the blocking shaft to move in an arc shape. At this time, the transmission ring can be toggled to move horizontally, thereby driving the L-shaped cover to move horizontally through the transmission ring, so that multiple specimen boxes can be easily moved into the equipment box, and the L-shaped cover can be used to seal the equipment box; S4. After multiple specimen boxes are moved into the equipment box, the insertion rods can correspond to the two slots respectively. Then, by releasing the insertion rods, the tension springs in the stressed state can drive the insertion rods to move downward, so that the insertion rods are inserted into the corresponding slots to position the rotating wheel. At this time, the L-shaped cover can be positioned through the transmission of the structure; S5. Use a camera to photograph the test sample, and then transmit the photographed image to the immunofluorescence instrument. Use the immunofluorescence instrument to perform data analysis on the photographed test sample, and then display the analyzed data results. When photographing the test sample, multiple fill lights can be turned on to prevent light interference when photographing the test sample. S6. When the entire equipment is operated by hand, the strap can be put on the operator's wrist, and then the strap can be contracted by pushing the ring, and the operator's wrist can be tied with the strap. Then, the adjusting screw is rotated to drive the transmission plate downward under the action of the threaded transmission with the nut. When the transmission plate moves downward, the two transmission rods can drive the pressure rod to move downward, and the pressure rod can be used to squeeze the elastic plates on both sides, so that the two elastic plates are deformed and drive the corresponding splints to move, so that the two splints clamp and position the strap, so that the strap can be stably tied to the operator's wrist.

[0015] It should be understood that the foregoing general description and the following detailed description are exemplary only and are not restrictive of the invention.

[0016] Beneficial effects: In the present invention, by providing a specimen supporting mechanism, after the sampled test specimen is placed in the specimen box, the corresponding multiple L-shaped pressing pieces can be driven to rotate by flipping the adjustment shafts on both sides, so that one side of the L-shaped pressing piece moves into the specimen box, pressing and positioning the test specimen, so that the test specimen will not shake in the specimen box, which is convenient for accurate detection of the test specimen. At the same time, multiple specimen boxes are provided, which can simultaneously detect multiple identical test specimens at a time, thereby obtaining multiple groups of data, which is convenient for horizontal comparison, thereby improving the accuracy of detection. In the present invention, by means of the detection mechanism, a specimen box loaded with a test sample can be moved into an equipment box. At this time, the test sample will correspond to the corresponding camera position. At this time, the test sample can be photographed by the camera, and then the photographed image can be transmitted to the immunofluorescence instrument. The immunofluorescence instrument is used to perform data analysis on the photographed test sample, and then the analyzed data results can be displayed. In addition, when photographing the test sample, multiple fill lights can be turned on, so that the test sample will not be disturbed by light when photographing. In the present invention, the transmission mechanism is set up, and the rotating shaft can be rotated by rotating the rotating wheel, and the swing arm can be driven to rotate. When the swing arm rotates, the blocking shaft can be driven to move in an arc shape, and the transmission ring can be turned to move horizontally. The transmission ring can drive the L-shaped cover to move horizontally, so that multiple specimen boxes can be moved in or out of the equipment box conveniently. In the present invention, by providing a fixing mechanism, the strap can be put on the operator's wrist when the operator holds the sheath, and then the strap can be contracted by pushing the ring, and the operator's wrist can be tied with the strap. In this way, when the operator holds the device with one hand, the strap can form a stable connection, so that when slipping occurs, the entire device will not fall to the ground.

[0017] The present invention is provided with multiple specimen boxes and multiple detection mechanisms, which can detect multiple test specimens of the same sampling, thereby obtaining multiple groups of data at the same time, facilitating horizontal comparison and improving the accuracy of data analysis. At the same time, when the operator holds the device in hand for operation, it can maintain a stable connection with the operator's wrist, thereby preventing the entire device from falling and avoiding damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A three-dimensional schematic diagram of the first-perspective structure of an apparatus for performing antinuclear antibody testing using immunofluorescence method according to an embodiment of the present invention; Figure 2 A schematic diagram of the structure of a device for performing antinuclear antibody testing using immunofluorescence method according to an embodiment of the present invention from a second perspective; Figure 3 A three-dimensional schematic diagram of the connection structure of the rotating shaft, swing arm, stop shaft, transmission ring, and L-shaped cover plate of the device for performing antinuclear antibody testing using the immunofluorescence method provided by an embodiment of the present invention; Figure 4 A three-dimensional schematic diagram of the connection structure between the support plate and the L-shaped cover plate of the device for performing antinuclear antibody testing using immunofluorescence method provided by an embodiment of the present invention; Figure 5A three-dimensional schematic diagram of a specimen box, two adjustment shafts, and a plurality of L-shaped pressing pieces connecting structures of an apparatus for performing antinuclear antibody testing using immunofluorescence method provided by an embodiment of the present invention; Figure 6 A three-dimensional schematic diagram of the connection structure of the adjustment shaft, multiple L-shaped pressing pieces, and the rotating plate of the device for performing antinuclear antibody testing using the immunofluorescence method provided by an embodiment of the present invention; Figure 7 A three-dimensional schematic diagram of the connection structure of a collar, two clamping plates, an adjusting screw, two transmission rods, and a pressure rod of a device for performing antinuclear antibody testing using an immunofluorescence method provided by an embodiment of the present invention; Figure 8 A three-dimensional schematic diagram of the connection structure of a support frame, multiple fill lights, a camera, a transmission wire and a display screen of an apparatus for performing antinuclear antibody testing using immunofluorescence method provided by an embodiment of the present invention.

[0019] Reference numerals: 1. Equipment box; 2. L-shaped cover; 3. Support plate; 4. Limit plate; 5. Limit slot; 6. Specimen box; 7. Adjustment shaft; 8. L-shaped pressure plate; 9. Rotating plate; 10. Clamping shaft; 11. Elastic clip; 12. Support frame; 13. Mounting cover; 14. Fill light; 15. Camera; 16. Conveyor wire; 17. Immunofluorescence instrument; 18. Seal; 19. Rotating shaft; 20. Swing lever; 21. Stop shaft ; 22. Transmission ring; 23. Rotating wheel; 24. Insert rod; 25. Tension spring; 26. Positioning ring; 27. Slot; 28. Pad; 29. ​​Handle; 30. Mounting plate; 31. Sheath; 32. Mounting frame; 33. Connecting plate; 34. Strap; 35. Ring; 36. Clamp; 37. Elastic plate; 38. Transmission rod; 39. Pressure rod; 40. Transmission plate; 41. Adjusting screw; 42. Nut. DETAILED DESCRIPTION

[0020] The embodiments of the present invention are described below with reference to the accompanying drawings. In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms, "connection", and "installation" should be understood in a broad sense. For example, "connection" can be a detachable connection or a non-detachable connection; it can be a direct connection or an indirect connection through an intermediate medium. In addition, "communication" can be a direct connection or an indirect connection through an intermediate medium. Here, "fixed" means that the two are connected to each other and the relative position relationship after connection remains unchanged. The directional terms mentioned in the embodiments of the present invention, such as "inside", "outside", "top", "bottom", etc., are only reference to the directions of the accompanying drawings. Therefore, the directional terms used are for better and clearer explanation and understanding of the embodiments of the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present invention.

[0021] In the embodiments of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Therefore, features defined as "first" or "second" may explicitly or implicitly include one or more of the features.

[0022] In the embodiments of the present invention, "and / or" is simply a description of the association relationship between associated objects, indicating that three possible relationships exist. For example, "A and / or B" can represent three situations: A exists alone, A and B exist simultaneously, and B exists alone. Furthermore, the character " / " in this document generally indicates that the associated objects are in an "or" relationship.

[0023] References to "one embodiment" or "some embodiments" in this specification mean that a particular feature, structure, or characteristic described in connection with that embodiment is included in one or more embodiments of the present invention. Thus, phrases such as "in one embodiment," "in some embodiments," "in other embodiments," and "in yet other embodiments" appearing in various places in this specification do not necessarily refer to the same embodiment, but rather mean "one or more, but not all, embodiments," unless otherwise specifically emphasized. The terms "including," "comprising," "having," and variations thereof mean "including but not limited to," unless otherwise specifically emphasized.

[0024] Example 1: Reference Figures 1-8, a device for antinuclear antibody testing using immunofluorescence method according to this embodiment includes an equipment box 1, a mounting plate 30 is fixedly mounted on the top of the equipment box 1, a handle 29 is fixed on the mounting plate 30, and a sheath 31 is covered on the handle 29 to increase the comfort when holding. Two symmetrical pads 28 are provided at the bottom of the equipment box 1 to ensure the stability of the device when placed. Inside the equipment box 1, a support plate 3 is provided, and the support plate 3 is used to install the detection mechanism. In addition, an L-shaped cover plate 2 is slidably connected to the inside of the equipment box 1, and a seal 18 is tightly fitted on one side of the L-shaped cover plate 2. The seal 18 is tightly fitted to the inner wall of the equipment box 1 to ensure that the L-shaped cover plate 2 can seal the equipment box 1 when closed.

[0025] like Figure 4 and Figure 5 As shown, on the L-shaped cover plate 2, multiple specimen supporting mechanisms are installed at equal intervals. Each specimen supporting mechanism includes a specimen box 6 fixedly mounted on the L-shaped cover plate 2, and both sides of the specimen box 6 are rotatably connected with an adjustment shaft 7. A plurality of L-shaped pressing pieces 8 are fixedly sleeved at equal intervals on the adjustment shaft 7, and one side of the L-shaped pressing piece 8 extends into the specimen box 6 for pressing the test specimen. When in use, the test specimen after sampling is placed in the specimen box 6, and then the adjustment shafts 7 on both sides are flipped to drive the corresponding multiple L-shaped pressing pieces 8 to rotate so that one side of the L-shaped pressing piece 8 moves into the specimen box 6, and the test specimen is pressed and positioned to prevent the test specimen from shaking in the specimen box 6, thereby ensuring the accuracy of the test. At the same time, multiple specimen boxes 6 are provided, so that multiple identical test specimens can be tested simultaneously at a single time, which is convenient for horizontal comparison and improves the accuracy during detection.

[0026] One end of the adjustment shaft 7 is fixedly mounted with a rotating plate 9, which has a mounting opening, in which an elastic clip 11 is fixedly mounted, and a clamping shaft 10 is movably mounted in the elastic clip 11, one end of which is fixedly mounted on one side of the specimen box 6. The adjustment shaft 7 can be rotated by flipping the rotating plate 9, and when the test specimen is pressed and positioned, the elastic clip 11 will be clamped with the corresponding clamping shaft 10 to position the rotating plate 9, thereby limiting the adjustment shaft 7 and maintaining the clamping force of the L-shaped pressing piece 8 on the test specimen.

[0027] like Figure 4 and Figure 8As shown, multiple detection mechanisms are installed at equal intervals on one side of the support plate 3. Each detection mechanism includes a support frame 12 fixedly mounted on one side of the support plate 3. A mounting cover 13 is fixedly mounted on the support frame 12. A camera 15 is fixedly mounted on the top inner wall of the mounting cover 13. A plurality of fill lights 14 are fixedly mounted at equal intervals on the bottom of the support frame 12. A transmission wire 16 is electrically connected to the camera 15. The top of the transmission wire 16 extends above the equipment box 1 and is electrically connected to an immunofluorescence instrument 17, which is fixedly mounted on the top of the equipment box 1. During use, a specimen box 6 loaded with a test sample is moved into the equipment box 1. At this time, the test sample will correspond to the position of the corresponding camera 15. The test sample is photographed using the camera 15, and the photographed image is then transmitted to the immunofluorescence instrument 17. The immunofluorescence instrument 17 performs data analysis on the photographed test sample and displays the analyzed data results. At the same time, when photographing the test sample, the multiple fill lights 14 are lit to ensure that the photography is not disturbed by light.

[0028] In addition, if Figure 4 As shown, two limit plates 4 are symmetrically fixed to the bottom of the support plate 3. Two limit slots 5 are symmetrically formed on the L-shaped cover plate 2. The bottoms of the limit plates 4 extend into the corresponding limit slots 5 and are slidably connected to the inner walls of the limit slots 5. The sliding connection between the limit plates 4 and the limit slots 5 limits the lateral sliding of the L-shaped cover plate 2, ensuring that the L-shaped cover plate 2 can maintain lateral linear movement.

[0029] Finally, a fixing mechanism is provided on the handle 29 for tying to the staff's arm, making it easier for the staff to hold and move the device.

[0030] like Figure 3 As shown, the transmission mechanism includes a rotating shaft 19 that extends through and is rotatably connected to the top inner wall of the equipment box 1. A rotating wheel 23 is fixedly mounted to the top of the rotating shaft 19 to facilitate rotation by the operator. A swing arm 20 is fixedly mounted to the bottom end of the rotating shaft 19, and a stop shaft 21 is fixedly mounted to one side of the bottom of the swing arm 20. A transmission ring 22 is fixedly mounted on the L-shaped cover plate 2, located on one side of the support plate 3. The bottom end of the stop shaft 21 extends into the transmission ring 22, engaging with the transmission ring 22.

[0031] When the operator rotates the rotating wheel 23, it drives the rotating shaft 19 to rotate, which in turn drives the swing arm 20 to swing. As the swing arm 20 swings, the blocking shaft 21 moves in an arc, shifting the transmission ring 22 to move laterally. Because the transmission ring 22 is fixedly connected to the L-shaped cover 2, the L-shaped cover 2 also moves laterally, facilitating the movement of multiple specimen boxes 6 in and out of the equipment box 1.

[0032] In order to ensure that the wheel 23 can maintain a stable position after rotation, Figure 2 and Figure 3 As shown, a positioning ring 26 is fixedly mounted on the top of the equipment box 1, with the rotating shaft 19 extending through the center of the positioning ring 26. Two symmetrical slots 27 are formed on the top of the positioning ring 26, with the two slots 27 arranged at a 90° angle. A rod 24 is slidably connected to the rotating wheel 23 and engages with the two slots 27. A tension spring 25 is sleeved on the rod 24 and positioned above the rotating wheel 23. The top and bottom ends of the tension spring 25 are fixedly connected to the top of the rod 24 and the top of the rotating wheel 23, respectively.

[0033] When moving multiple specimen boxes 6 into or out of the device case 1, the insertion rod 24 can be aligned with the two slots 27. At this point, the operator can release the insertion rod 24. Under the elastic force of the tension spring 25, the insertion rod 24 moves downward and inserts into the corresponding slot 27, positioning the rotating wheel 23. This structural transmission can thus position the L-shaped cover 2, ensuring that it remains in a stable position after movement.

[0034] Example 2: Reference Figure 1 、 Figure 2 and Figure 7 This embodiment, based on the first embodiment, relates to the device for performing antinuclear antibody testing using the immunofluorescence method proposed in the first embodiment. The fixing mechanism includes a mounting bracket 32 ​​fixedly mounted on the handle 29. A connecting plate 33 is rotatably connected to the mounting bracket 32. A strap 34 is fixedly mounted on one side of the connecting plate 33. The strap 34 is designed to be worn around the operator's wrist. A loop 35 is mounted on the strap 34. A clamping assembly is mounted within the loop 35 to clamp the strap 34 in place.

[0035] like Figure 7 As shown, the clamping assembly includes two clamping plates 36 symmetrically slidably connected in the collar 35, and an elastic plate 37 fixedly mounted on the bottom of the clamping plates 36. The bottom of the elastic plate 37 is fixedly mounted on the bottom inner wall of the collar 35. Two transmission rods 38 are symmetrically and slidably connected through the top inner wall of the collar 35, and the bottom ends of the two transmission rods 38 are fixedly mounted with a same pressure rod 39. The pressure rod 39 is used to press the two elastic plates 37 so that the elastic plates 37 undergo elastic deformation. The top ends of the two transmission rods 38 are fixedly mounted with a same transmission plate 40, and the top of the transmission plate 40 is fixedly connected with a nut 42. The top of the collar 35 is rotatably connected with an adjusting screw 41, which passes through the transmission plate 40 and the nut 42 respectively and is threadedly connected to the nut 42.

[0036] When the operator holds the sheath 31, the strap 34 can be put on the wrist. Then, the strap 34 is contracted by pushing the ring 35. When it is contracted to an appropriate degree, the adjusting screw 41 can be turned. Under the threaded transmission action of the adjusting screw 41 and the nut 42, the transmission plate 40 will be driven to move downward. When the transmission plate 40 moves downward, the pressure rod 39 will be driven downward through the two transmission rods 38. When the pressure rod 39 moves downward, it will squeeze the elastic plates 37 on both sides, causing the elastic plates 37 to deform and drive the corresponding splints 36 to move. Finally, the two splints 36 will clamp and position the strap 34 to ensure that the strap 34 can be stably tied to the operator's wrist.

[0037] In this way, when the operator holds the device with one hand, the strap 34 can form a stable connection to prevent the device from falling to the ground when it slips, thereby ensuring the safety and stability of the operation.

[0038] The present invention provides a method for testing antinuclear antibodies using immunofluorescence, which is applied to the above-mentioned device for testing antinuclear antibodies using immunofluorescence, and comprises the following steps: S1. Place the collected multiple test samples in the corresponding specimen box 6 in sequence, then flip the corresponding two rotating plates 9 to drive the two adjusting shafts 7 to rotate, thereby driving the corresponding multiple L-shaped pressing pieces 8 to rotate, so that one side of the L-shaped pressing piece 8 moves into the specimen box 6, pressing and positioning the test specimens, so that the test specimens will not shake in the specimen box 6; S2. When the test specimen is pressed and positioned, the elastic clip 11 can be clamped with the corresponding clamping shaft 10, so that the rotating plate 9 can be positioned, thereby limiting the adjustment shaft 7 and further maintaining the clamping force of the L-shaped pressing piece 8 on the test specimen. S3. The rotating wheel 23 is rotated to drive the rotating shaft 19 to rotate, which in turn drives the swinging rod 20 to rotate. When the swinging rod 20 rotates, the blocking shaft 21 can be driven to move in an arc shape. At this time, the transmission ring 22 can be turned to move laterally. The transmission ring 22 can drive the L-shaped cover plate 2 to move laterally, thereby facilitating the movement of multiple specimen boxes 6 into the equipment box 1 and allowing the L-shaped cover plate 2 to seal the equipment box 1. S4. After the multiple specimen boxes 6 are moved into the equipment box 1, the insertion rod 24 can correspond to the positions of the two slots 27 respectively. Then, by releasing the insertion rod 24, the tension spring 25 in the stressed state can drive the insertion rod 24 to move downward, so that the insertion rod 24 is inserted into the corresponding slot 27, positioning the rotating wheel 23. At this time, the L-shaped cover plate 2 can be positioned through the transmission of the structure; S5. Use the camera 15 to photograph the test sample, and then transmit the photographed image to the immunofluorescence instrument 17. Use the immunofluorescence instrument 17 to perform data analysis on the photographed test sample, and then display the analyzed data results. When photographing the test sample, multiple fill lights 14 can be turned on to prevent light interference when photographing the test sample. S6. When the entire device is operated by hand, the strap 34 can be put on the operator's wrist, and then the strap 34 can be contracted by pushing the ring 35, and the operator's wrist can be tied with the strap 34. Then, the adjusting screw 41 is rotated and the transmission plate 40 is driven to move downward under the threaded transmission action of the nut 42. When the transmission plate 40 moves downward, the two transmission rods 38 can drive the pressure rod 39 to move downward, and the pressure rod 39 can be used to squeeze the elastic plates 37 on both sides, so that the two elastic plates 37 are deformed and drive the corresponding splints 36 to move, so that the two splints 36 can clamp and position the strap 34, so that the strap 34 can be stably tied to the operator's wrist.

[0039] However, as is well known to those skilled in the art, the working principles and wiring methods of the fill light 14, camera 15, transmission wire 16 and immunofluorescence analyzer 17 are commonplace, and are all conventional means or common knowledge, and will not be elaborated here. Those skilled in the art can make any selections according to their needs or convenience.

[0040] The above are only specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. The embodiments of the present invention and the features therein can be combined with each other unless there is a conflict. Therefore, the scope of protection of the present invention shall be based on the scope of protection of the claims.

Claims

1. A device for performing antinuclear antibody testing using an immunofluorescence method, comprising an equipment box (1), a handle (29) fixedly mounted on the equipment box (1), a protective sleeve (31) fixedly mounted on the handle (29), a mounting plate (30) fixedly mounted on one side of the handle (29), the mounting plate (30) fixedly mounted on the top of the equipment box (1), two pads (28) symmetrically fixedly mounted on the bottom of the equipment box (1), a support plate (3) fixedly mounted inside the equipment box (1), an L-shaped cover plate (2) slidably connected inside the equipment box (1), a seal (18) fixedly mounted on one side of the L-shaped cover plate (2), the seal (18) tightly fitting with the inner wall of the equipment box (1), one side of the L-shaped cover plate (2) extending to the outside of the equipment box (1), and a seal (18) fixedly mounted on one side of the L-shaped cover plate (2), characterized in that: The device also includes: A plurality of specimen supporting mechanisms, wherein the plurality of specimen supporting mechanisms are installed at equal intervals on the L-shaped cover plate (2), and the specimen supporting mechanisms are used to carry the test specimens; A plurality of detection mechanisms, the plurality of detection mechanisms being installed at equal intervals on one side of the support plate (3), the tops of the detection mechanisms extending above the device box (1) and being installed on the top of the device box (1); A transmission mechanism, the transmission mechanism passes through the top inner wall of the equipment box (1) and is connected to the top inner wall of the equipment box (1), and the bottom of the transmission mechanism is connected to the L-shaped cover plate (2); A fixing mechanism is mounted on the handle (29) and is used for binding with the arm of the staff.

2. The device for antinuclear antibody testing using immunofluorescence method according to claim 1, characterized in that: The specimen support mechanism comprises a specimen box (6) fixedly mounted on the L-shaped cover plate (2), both sides of the specimen box (6) being rotatably connected to adjustment shafts (7), a plurality of L-shaped pressing plates (8) being fixedly sleeved at equal intervals on the adjustment shafts (7), one side of the L-shaped pressing plates (8) extending into the specimen box (6), and the L-shaped pressing plates (8) being used to press the test specimens.

3. The device for antinuclear antibody testing using immunofluorescence method according to claim 2, characterized in that: A rotating plate (9) is fixedly mounted on one end of the adjusting shaft (7), the rotating plate (9) is provided with a mounting opening, an elastic clip (11) is fixedly mounted in the mounting opening, a clamping shaft (10) is movably mounted in the elastic clip (11), and one end of the clamping shaft (10) is fixedly mounted on one side of the specimen box (6).

4. The device for antinuclear antibody testing using immunofluorescence method according to claim 1, characterized in that: The detection mechanism comprises a support frame (12) fixedly mounted on one side of a support plate (3); a mounting cover (13) fixedly mounted on the support frame (12); a camera (15) fixedly mounted on the top inner wall of the mounting cover (13); a plurality of fill lights (14) fixedly mounted at equal intervals on the bottom of the support frame (12); a transmission wire (16) electrically connected to the camera (15); a top end of the transmission wire (16) extends to the top of the equipment box (1) and is electrically connected to an immunofluorescence instrument (17); and the immunofluorescence instrument (17) is fixedly mounted on the top of the equipment box (1).

5. The device for antinuclear antibody testing using immunofluorescence method according to claim 1, characterized in that: Two limit plates (4) are symmetrically fixedly mounted on the bottom of the support plate (3); two limit slots (5) are symmetrically provided on the L-shaped cover plate (2); the bottoms of the limit plates (4) extend into the corresponding limit slots (5) and are slidably connected to the inner walls of the limit slots (5).

6. The device for antinuclear antibody testing using immunofluorescence method according to claim 1, characterized in that: The transmission mechanism comprises a rotating shaft (19) penetrating through the top inner wall of the equipment box (1) and rotatably connected to the top inner wall of the equipment box (1); a rotating wheel (23) is fixedly mounted on the top of the rotating shaft (19); a swing rod (20) is fixedly mounted on the bottom end of the rotating shaft (19); a stopper shaft (21) is fixedly mounted on one side of the bottom of the swing rod (20); a transmission ring (22) located on one side of the support plate (3) is fixedly mounted on the L-shaped cover plate (2); the bottom end of the stopper shaft (21) extends into the transmission ring (22); and the stopper shaft (21) and the transmission ring (22) are in transmission cooperation.

7. The device for antinuclear antibody testing using immunofluorescence method according to claim 6, characterized in that: A positioning ring (26) is fixedly installed on the top of the equipment box (1), the rotating shaft (19) passes through the center point of the positioning ring (26), two slots (27) are symmetrically opened on the top of the positioning ring (26), and the angle between the two slots (27) is 90 degrees. A plug rod (24) passes through the rotating wheel (23) and is slidably connected. The plug rod (24) is movably engaged with the two slots (27) respectively. A tension spring (25) located above the rotating wheel (23) is sleeved on the plug rod (24), and the top and bottom ends of the tension spring (25) are fixedly connected to the top of the plug rod (24) and the top of the rotating wheel (23) respectively.

8. The device for antinuclear antibody testing using immunofluorescence method according to claim 1, characterized in that: The fixing mechanism comprises a mounting frame (32) fixedly mounted on the handle (29), a connecting plate (33) being rotatably connected to the mounting frame (32), a strap (34) being fixedly mounted on one side of the connecting plate (33), the strap (34) being used to be mounted on the wrist of an operator, a collar (35) being mounted on the strap (34), a clamping assembly being mounted in the collar (35), the top of the clamping assembly extending to the top of the collar (35) and being connected to the top of the collar (35).

9. The device for antinuclear antibody testing using immunofluorescence method according to claim 8, characterized in that: The clamping mechanism comprises two clamping plates (36) symmetrically slidably connected in the collar (35), and the two clamping plates (36) are used to clamp and position the binding belt (34); an elastic plate (37) is fixedly installed on one side of the two clamping plates (36) close to each other; the bottoms of the two elastic plates (37) are fixedly installed on the bottom inner wall of the collar (35); two transmission rods (38) are symmetrically penetrated and slidably connected on the top inner wall of the collar (35); the bottom ends of the two transmission rods (38) are fixedly installed with a same pressing rod (39); the pressing rod (39) is used to press the two elastic plates (37) so that the two elastic plates (37) undergo elastic deformation; the top ends of the two transmission rods (38) are fixedly installed with a same transmission plate (40); the top of the transmission plate (40) is fixedly connected with a nut (42); the top of the collar (35) is rotatably connected with an adjusting screw (41); the adjusting screw (41) penetrates the transmission plate (40) and the nut (42) respectively and is threadedly connected to the nut (42).

10. A method for testing antinuclear antibodies using immunofluorescence, applied to the device for testing antinuclear antibodies using immunofluorescence as claimed in any one of claims 1 to 9, characterized in that: The following steps are involved: S1, sequentially placing the collected multiple test samples in the corresponding specimen box (6), then turning over the corresponding two rotating plates (9) to drive the two adjustment shafts (7) to rotate, thereby driving the corresponding multiple L-shaped pressing pieces (8) to rotate, so that one side of the L-shaped pressing piece (8) is moved into the specimen box (6), and the test specimen is pressed and positioned, so that the test specimen will not shake in the specimen box (6); S2. When the test specimen is pressed and positioned, the elastic clamp (11) can be clamped with the corresponding clamping shaft (10), so that the rotating plate (9) can be positioned, thereby limiting the adjustment shaft (7), and further maintaining the clamping force of the L-shaped pressing piece (8) on the test specimen without change; S3, by rotating the rotating wheel (23), the rotating shaft (19) is driven to rotate, and the swinging rod (20) is driven to rotate. When the swinging rod (20) rotates, the blocking shaft (21) is driven to move in an arc shape, and the transmission ring (22) is driven to move horizontally, so that the L-shaped cover plate (2) can be driven to move horizontally through the transmission ring (22), so that a plurality of specimen boxes (6) can be conveniently moved into the equipment box (1), and the L-shaped cover plate (2) can be used to seal the equipment box (1); S4, after the plurality of specimen boxes (6) are moved into the equipment box (1), the insertion rod (24) can correspond to the positions of the two slots (27) respectively, and then the insertion rod (24) is released, and the tension spring (25) in a stressed state can drive the insertion rod (24) to move downward, so that the insertion rod (24) is inserted into the corresponding slot (27), and the rotating wheel (23) is positioned. At this time, the L-shaped cover plate (2) can be positioned through the transmission of the structure; S5, photographing the test sample using the camera (15), and then transmitting the photographed image to the immunofluorescence instrument (17), performing data analysis on the photographed test sample using the immunofluorescence instrument (17), and then displaying the analyzed data results, and when photographing the test sample, multiple fill lights (14) can be turned on, so that the test sample will not be disturbed by light when photographing it; S6. When the whole device is operated by hand, the strap (34) can be put on the wrist of the operator, and then the strap (34) can be contracted by pushing the ring (35), and the wrist of the operator can be tied with the strap (34). Then, the adjusting screw (41) is rotated to drive the transmission plate (40) downward under the threaded transmission action of the nut (42). When the transmission plate (40) moves downward, the pressure rod (39) can be driven downward by the two transmission rods (38), and the pressure rod (39) can be used to squeeze the elastic plates (37) on both sides, so that the two elastic plates (37) are deformed and drive the corresponding clamping plates (36) to move, so that the two clamping plates (36) clamp the strap (34) to position, so that the strap (34) can be stably tied to the wrist of the operator.

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