Method for quantifying gonad quantity of scylla paramamosain and nondestructive testing device for gonad of scylla paramamosain

Abstract

The invention discloses a method for detecting the gonad quantity of scylla paramamosain, and particularly relates to a method for quantifying the gonad quantity of scylla paramamosain and a nondestructive testing device for the gonad of scylla paramamosain. The method comprises the following steps: a, selecting artificially bred female scylla paramamosain, and acquiring gonad distribution photos of different scylla paramamosain at gonad development stages by utilizing an infrared imaging technology; b, calculating the ratio of the gonad distribution area to the crab shell area in each photo obtained in the step a; c, weighing the scylla paramamosain selected in the step a, performing anesthesia sampling, then weighing the weight of gonad tissue, and calculating a gonad index (GSI); and d, by taking the gonad index (GSI) in the step c as an x axis and the gonad area ratio in the step b as a y axis, establishing a quantitative linear model of the gonad quantity of the scylla paramamosain, and fitting a curve function as y = 1.1835 x + 32.646. The technical problem of'accurate detection and quantification of the sexual gland of the scylla paramamosain 'is solved, and the method has practical significance in the aspects of quality selection of the scylla paramamosain and screening of breeding crabs and butter crabs.

Description

Technical Field

[0001] The invention relates to a method for detecting the amount of gonads of mud crabs, in particular to a method for quantifying the amount of gonads of mud crabs. The invention also provides a non-destructive detection device for the gonads of mud crabs. Background Art

[0002] The mud crab is one of the important marine economic crabs in my country. It has the advantages of fast growth, delicious meat and high nutritional value. It occupies an important position in aquaculture and market sales. Its industry scale is large and continues to develop. Accurate detection and quantification of its gonads plays a key role in evaluating the reproductive status, reproductive capacity and overall quality of crabs, which is directly related to the economic benefits of the industry. As people's requirements for the yield and quality of mud crabs continue to increase, farmers need more accurate and efficient methods to understand the gonad development of mud crabs in order to optimize breeding management and improve reproductive efficiency and crab seedling quality. This provides an industrial demand background for the research of this patented technology.

[0003] Therefore, the applicant proposed the present invention. Summary of the invention

[0004] The purpose of the present invention is to solve the above-mentioned deficiencies of the prior art and provide a method for quantifying the amount of gonads of Scylla pseudoburrowing.

[0005] In order to achieve the above object, a method for quantifying the amount of gonads of mud crabs designed by the present invention comprises the following steps: a. Select artificially reared female mud crabs and use infrared imaging technology to obtain photos of the gonad distribution of mud crabs at different gonad development stages; b. Calculating the ratio of the area of ​​the gonad distribution in each photo obtained in step a to the area of ​​the crab shell, specifically comprising the following steps: S1. Import the photos obtained in step a into ImageJ software; S2. Use the “Polygon selections” function in ImageJ software to outline the contours of the gonad distribution and the crab shell respectively; S3. Use the “Measure” function in ImageJ software to calculate the area of ​​the gonad outline and crab shell outline respectively; S4. Calculate the ratio of the gonad distribution area to the crab shell area using the following formula (1): Area ratio (%) = (S1 (gonad area)) / (S2 (crab shell area)) × 100% (1); c. Weigh and anesthetize each mud crab selected in step a, then weigh the weight of the gonadal tissue and calculate the gonadal index (GSI). The gonadal index (GSI) is calculated using the following formula (2): Gonad index (GSI) (%) = (gonad weight) / (blue crab weight) × 100% (2); d. Using the gonad index (GSI) in step c as the x-axis and the gonad area ratio in step b as the y-axis, a quantitative linear model for the gonad volume of Scylla pseudoburrowing was established, and the fitting curve function was y = 1.1835x + 32.646.

[0006] At the same time, the present invention also provides a non-destructive detection device for the gonads of the mud crab used in the aforementioned method for quantifying the gonadal amount of the mud crab, and the structure thereof comprises: A light-proof box, wherein an opening is provided on the front of the box, and the opening is covered by a movable plate hinged to the box; a semicircular opening is provided below the movable plate for the arm to pass through; and, infrared camera; The infrared camera is placed in the box, mounted to the top of the box through a bracket, and the shooting angle is toward the bottom of the box.

[0007] Infrared imaging technology has been widely used in many fields. Infrared cameras can capture infrared rays emitted by objects. By analyzing infrared rays, information such as the temperature distribution and internal structure of the object can be obtained. Since the gonads of blue crabs will be accompanied by changes in metabolic activity during development, resulting in different heat distributions, the above-mentioned detection device uses the principle that infrared cameras can capture and analyze the temperature distribution and internal structure information presented by different heat distributions, and can clearly show the distribution and outline of the gonads of pseudo-burrowing blue crabs, thereby improving the accuracy of detection.

[0008] The hinged structure of the box body and the movable plate is preferably selected as follows: the movable plate is connected to the device box body through hinge 1 and hinge 2, and is opened and closed outside the box body.

[0009] Furthermore, in the above-mentioned non-destructive detection device for the gonads of the mud crab, the shooting angle of the infrared camera in its structure is adjustable to facilitate capturing the mud crab for photographing.

[0010] Compared with the prior art, the method for quantifying the amount of gonads of mud crabs and the non-destructive detection device for gonads of mud crabs obtained by the present invention have the following technical effects: The infrared imaging technology and the established linear model of gonad index (GSI) and gonad area ratio can quantify and predict the amount of gonads and specific gonad index, which has practical significance in the quality selection of mud crabs and the screening of seed crabs and butter crabs. In addition, this detection device and quantification method have important theoretical significance in the identification of the ovarian development stage of mud crabs, ovarian maturation and reproduction and ovulation.

[0011] In addition, the infrared imaging-based nondestructive detection device for the gonads of the mud crab of the present invention has the following advantages over the traditional gonad detection device / method: (1) Protecting the life of the crab: The detection device of the present invention can observe the development of the gonads without damaging the crab, so that the crab can continue to survive after the detection; (2) Improving the detection accuracy: Traditional detection devices / methods such as irradiation with strong light or detection in a device that can emit strong light cannot truly reflect the distribution status of the crab gonads, especially the gonad contours inside it. The detection device of the present invention uses the principle that an infrared camera can capture and analyze the temperature distribution and internal structure information presented by different heat distributions, and can clearly show the distribution and contours of the crab gonads, thereby improving the accuracy of the detection; (3) Convenient operation: The operation process is simplified, and the detection results can be quickly obtained. The image is transmitted to an external device in real time for rapid analysis, thereby improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The figure shows a front schematic diagram of a nondestructive detection device for the gonad of a mud crab based on infrared imaging provided by the present invention; Figure 2 Shown Figure 1 perspective drawing; Figure 3 Shown Figure 1 Perspective view of the movable area after opening; Figure 4 Shown is a front view of the infrared camera; Figure 5 Shown are the distribution of the gonads of blue crabs photographed using the device (A and B) and the corresponding anatomical diagram of the blue crab; Figure 6 Shown is a schematic diagram of the gonad distribution area (represented by a single line) and crab shell area (represented by a dotted line) outlined by ImageJ software; Figure 7 Shown is a graph of a linear model based on the gonadal index (GSI) (x-axis) and the gonadal area ratio (y-axis).

[0013] In the figure: 1. Box body; 2. Movable plate; 3. Hinge 1; 4. Hinge 2; 5. Semicircular opening; 6. Data cable; 7. USB port; 8. Infrared camera; 9. Camera lens; 10. LED lamp beads; 11. Light-sensitive sensing area; 12. Bracket. DETAILED DESCRIPTION

[0014] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the present invention.

[0015] See also Figure 1-3 The present embodiment provides a nondestructive detection device for the gonad of a pseudo-burrowing mud crab based on infrared imaging, which includes a box body 1, a movable plate 2, a hinge 1 3 and a hinge 2 4 for connecting the box body 1 and the movable plate 2, a semicircular opening 5 at the bottom of the movable plate 2, an infrared camera 8, a data cable 6 connecting the infrared camera 8, and a USB interface 7.

[0016] The box 1 is a rectangular parallelepiped structure, and acrylic material is used in the selection of materials. The thickness of the acrylic plate is 5 mm and the size is 35 cm*25 cm*30 cm. Except for a movable plate 2 on the front, the other five sides are strictly sealed and opaque. In addition, the color of the acrylic material is black, which further reduces the light in the box 1 on the one hand, and avoids the reflection of the light in the box 1 on the other hand.

[0017] The movable plate 2 is arranged on the front of the box 1. The front of the box 1 is divided into two parts, the upper and lower parts, which are fixed and immovable, reducing unnecessary interference from light and the outside world. The lower part is the movable plate 2 connected to the box 1 through hinges 1 3 and 2 4. The movable plate 2 can be opened and closed outside the box 1, which is convenient for operators to place crabs, adjust infrared cameras 8 and clean up later. In addition, a semicircular opening 5 with a diameter of 16 cm is left at the bottom of the movable plate 2, which is convenient for operators to move their arms after putting crabs into the device.

[0018] Please continue reading Figure 4 The infrared camera 8 includes a camera lens 9, a focus knob 13, 6 infrared LED lamp beads 10, a light-sensitive sensing area 11, and screws 14 for mounting the infrared camera 8 on a bracket 12. The focus knob 13 is closely connected to the camera lens 9, and the focal length can be freely adjusted to obtain a clear image. In addition, three infrared LED lamp beads 10 are distributed on the left and right sides of the lens for emitting infrared rays. When the light-sensitive sensing area 11 senses that the light in the box 1 drops and reaches a certain value, the LED lamp beads 10 will be activated to emit infrared rays.

[0019] The infrared camera 8 is fixed to the upper center of the box body 1 by means of a bracket 12 and a screw 14. The screw 14 for connecting the camera 8 and the bracket 12 can adjust the shooting angle of the infrared camera 8 so that the lens faces directly downward.

[0020] One end of the data line 6 is connected to the infrared camera 8 , and the USB port 7 at the other end can be conveniently connected to an external device such as a computer. The image data collected by the infrared camera 8 is transmitted to the external device via the data line 6 .

[0021] The detection device of this embodiment has the following specific operations: S1: Take a live crab to be tested, wipe off the surface moisture and dirt, open the movable plate 2 of the detection device, and place the crab in the center of the bottom of the device; S2: Connect the USB interface to a computer or other device, open the camera image acquisition software, manually adjust the focus knob 13 of the infrared camera 8 after the image appears, and close the movable plate 2 after the image becomes clear; S3: After closing the movable plate 2, the light inside the box 1 becomes dim and is detected by the light-sensitive area 11. The 6 LED lamp beads 10 of the infrared camera 8 will automatically turn on to emit infrared light. Since the crab gonads will be accompanied by changes in metabolic activities during development, resulting in different heat distributions, a clear ovarian outline will be presented on the image software. Click to take the image and save it in the corresponding path to facilitate the subsequent quantitative evaluation of the gonad index and development level.

[0022] like Figure 5 A and B are the two crabs tested, and the gray-white area outline in the image is the distribution of the gonads. To further verify the accuracy and reliability of the device's detection, the two crabs were dissected and it was found that the distribution shape and area of ​​the gonads were the gray-white outline area of ​​the image detected by the device.

[0023] In summary, the detection device of this embodiment has the following advantages over the traditional gonad detection device / method: (1) Protecting the life of the crab: The detection device of this embodiment can observe the development of the gonad without damaging the crab, so that the crab can continue to survive after the detection; (2) Improving the detection accuracy: Traditional detection methods such as irradiation with strong light or detection in a device that can emit strong light cannot truly reflect the distribution status of the crab gonad, especially the gonad contour inside it. The detection device of this embodiment uses an infrared camera to capture and analyze the temperature distribution and internal structure information presented by different heat distribution, and can clearly show the distribution and contour of the crab gonad, thereby improving the accuracy of the detection; (3) Convenient operation: The operation process is simplified, and the detection results can be quickly obtained. The image is transmitted to an external device in real time for rapid analysis, thereby improving the detection efficiency.

[0024] In the process of detecting the gonads of the mud crab by the present method, in order to further quantify the amount of its gonads, a method for quantifying the amount of the gonads of the mud crab is proposed, which comprises the following steps: a. Selection of Scylla pseudoburrowing and acquisition of photos Using artificially reared female mud crabs as materials, 30 mud crabs with different gonad development stages were selected. According to the operating steps of detecting the gonads of mud crabs using the detection device described above, 30 photographs of the gonad distribution of mud crabs were obtained.

[0025] 2. Quantification of gonadal distribution area and acquisition of gonadal index (GSI) According to the infrared imaging principle, the ovary of the blue crab appears white in shape and distribution, so the ratio of the white area to the area of ​​the entire crab shell can reflect the amount of the blue crab gonads. The specific calculation method of the ratio of the gonad distribution area to the area of ​​the crab shell is as follows: S1. Open ImageJ software and import the photos taken by the infrared detection device; S2. Select the “Polygon selections” option to outline the distribution of the gonads (represented by a single line) and the crab shell (represented by a dotted line), as shown in the figure. Figure 6 As shown; S3. Use the “Measure” function in ImageJ software to calculate the area of ​​the gonad outline and crab shell outline respectively; S4. Calculate the ratio of the gonad distribution area to the crab shell area using the following formula (1): Area ratio (%) = (S1 (gonad area)) / (S2 (crab shell area)) × 100% (1) After the above steps were completed, the 30 blue crabs were weighed and anesthetized for sampling, and then the weight of the gonadal tissue was weighed to calculate the gonadal index (GSI). The gonadal index (GSI) was calculated using the following formula (2): Gonad index (GSI) (%) = (gonad weight) / (blue crab weight) × 100% (2) The body weight, gonad weight, gonad index and gonad area ratio of 30 blue crabs are shown in Table 1 below.

[0026] Table 1 Serial number Weight (g) Gonad weight (g) Gonadal index (%) Gonadal area ratio (%) 1 343.25 63.23 18.42 55.62 2 349.14 50.82 14.56 55.12 3 329.86 54.45 16.51 55.97 4 317.25 47.43 14.95 55.26 5 349.45 38.16 10.92 51.57 6 314.68 62.25 19.78 58.38 7 450.62 74.25 16.48 57.21 8 376.76 77.76 20.64 56.39 9 452.27 77.45 17.12 53.20 10 399.75 82.13 20.54 58.16 11 399.37 67.06 16.79 52.29 12 327.94 68.47 20.88 56.06 13 424.01 63.98 15.09 51.82 14 349.30 69.85 20.00 60.34 15 307.57 62.08 20.18 54.94 16 355.80 55.65 15.64 56.32 17 398.12 74.91 18.82 56.25 18 566.45 78.25 13.81 56.67 19 357.58 57.44 16.06 53.77 20 368.50 44.23 12.00 43.11 21 357.97 38.45 10.74 48.62 22 516.56 38.43 7.44 37.69 23 347.05 47.44 13.67 47.89 24 418.46 43.9 10.49 39.07 25 349.75 38.9 11.12 48.95 26 379.09 58.3 15.38 45.88 27 375.77 73.4 19.53 47.27 28 377.83 88.2 23.34 51.68 29 317.33 64.7 20.39 51.33 30 516.15 40 7.75 29.48 3. Model establishment of the relationship between the gonadal index (GSI) and gonadal area ratio of the mud crab In order to explore the relationship between the above-mentioned blue crab gonad index (GSI) and the gonad area ratio, a linear model was established in Excel with the gonad index as the x-axis and the gonad area ratio as the y-axis, as shown in the following figure: Figure 7 The results showed that the gonad index and gonad area ratio of 30 blue crabs conformed to the linear model, and the fitting curve function was y = 1.1835x + 32.646 (where x is the gonad index and y is the gonad area ratio).

[0027] To further test the reliability of the aforementioned method for quantifying the gonadal amount of mud crabs, the reliability of the established linear model based on the gonadal index (GSI) and the gonadal area ratio was tested. First, 6 mud crabs were randomly selected from artificially reared female mud crabs. According to the aforementioned detection device and method, 6 corresponding gonad detection photos were obtained, and the area of ​​gonadal distribution and the area of ​​crab shell were calculated in ImageJ software, and the gonadal area ratio was further calculated. Secondly, the gonadal index (GSI) of these 6 crabs was predicted according to the aforementioned linear model. Finally, the 6 mud crabs were weighed and dissected, and the gonadal tissues were weighed and the gonadal index was calculated. The relevant data are shown in Table 2.

[0028] Table 2 Relevant data and indicators of the six crabs used to test the reliability of the quantification method of gonads The results showed that the linear model based on the gonadal index (GSI) and the gonadal area ratio accurately quantified the gonadal index of blue crabs. The error between the gonadal index predicted by the model and the actual value was about 5.79%.

[0029] Therefore, the use of infrared imaging-based non-destructive detection device for the gonads of mud crabs and the established linear model of gonad index (GSI) and gonad area ratio can quantify and predict the amount of gonads and specific gonad index, which has practical significance in the quality selection of mud crabs and the screening of seed crabs and butter crabs. In addition, this detection device and quantification method have important theoretical significance in the identification of the ovarian development stage of mud crabs, ovarian maturation and reproduction and ovulation.

[0030] The present invention is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, all technical solutions that are the same or similar to those of the present application fall within the protection scope of the present invention.

Claims

1. A method for quantifying the amount of gonads in Scylla pseudoburrowing, characterized in that: The steps include: a. Select artificially reared female mud crabs and use infrared imaging technology to obtain photos of the gonad distribution of mud crabs at different gonad development stages; b. Calculating the ratio of the area of ​​the gonad distribution in each photo obtained in step a to the area of ​​the crab shell, specifically comprising the following steps: S1. Import the photos obtained in step a into ImageJ software; S2. Use the "Polygon selections" function in ImageJ software to outline the contours of the gonad distribution and the crab shell respectively; S3. Use the "Measure" function in ImageJ software to calculate the area of ​​the gonad outline and crab shell outline respectively; S4. Calculate the ratio of the gonad distribution area to the crab shell area using the following formula (1): Area ratio (%) = (S1 (gonad area)) / (S2 (crab shell area)) × 100% (1); c. Weigh and anesthetize each mud crab selected in step a, then weigh the weight of the gonadal tissue and calculate the gonadal index (GSI). The gonadal index (GSI) is calculated using the following formula (2): Gonad index (GSI) (%) = (gonad weight) / (blue crab weight) × 100% (2); d. Using the gonad index (GSI) in step c as the x-axis and the gonad area ratio in step b as the y-axis, a quantitative linear model for the gonad volume of Scylla pseudoburrowing was established, and the fitting curve function was y = 1.1835x + 32.

646.

2. The non-destructive detection device for the gonads of the mud crab used in the method for quantifying the gonadal amount of the mud crab according to claim 1, characterized in that: include: A light-proof box (1), wherein an opening is provided on the front of the box (1), and the opening is shielded by a movable panel (2) hinged to the box (1); a semicircular opening (5) is provided below the movable panel (2) for an arm to pass through; and, infrared camera (8); The infrared camera (8) is placed inside the box (1), and is mounted to the top of the box (1) via a bracket (12), with a shooting angle directed toward the bottom of the box (1).

3. The non-destructive detection device for the gonad of the mud crab according to claim 2, characterized in that: The shooting angle of the infrared camera (8) is adjustable.

4. A non-destructive detection device for the gonads of mud crabs according to claim 2 or 3, characterized in that The hinged structure of the box body (1) and the movable plate (2) is as follows: the movable plate (2) is connected to the box body (1) via hinge 1 (3) and hinge 2 (4), and is opened and closed toward the outside of the box body (1).

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