Medical dressing for promoting surgical wound healing and preparation method thereof

Abstract

The present invention belongs to the technical field of medical dressings, and in particular to a medical dressing for promoting surgical wound healing and a preparation method thereof. The medical dressing for promoting surgical wound healing comprises the following raw materials in parts by weight: 5-10 parts of carboxymethyl chitosan, 0.5-1 parts of Vaccaria cyclotide B, 0.8-1.3 parts of sea hail vegetable polysaccharide, 1-3 parts of modified montmorillonite, 0.5-1 parts of carbomer, 1-5 parts of glycerol, and 100-120 parts of water. The medical dressing of the present invention is added with components such as sea hail vegetable polysaccharide, Vaccaria cyclotide B, and modified montmorillonite, wherein the synergistic effect of sea hail vegetable polysaccharide and Vaccaria cyclotide B can promote wound healing, and the modified montmorillonite can improve the absorption capacity of the exudate of the medical dressing.

Description

Technical Field

[0001] The invention belongs to the technical field of medical dressings, and in particular relates to a medical dressing for promoting surgical wound healing and a preparation method thereof. Background Art

[0002] The skin is the largest organ in the human body and the first line of defense of the human immune system. In daily life, the skin often encounters various injuries and then forms trauma. After the skin is exposed to the air after trauma, it is susceptible to infection by most Gram-positive bacteria and a small number of Gram-negative bacteria, such as Staphylococcus aureus and Escherichia coli. These pathogens will multiply rapidly at the site of the wound, affecting the speed of wound healing, further damaging the skin barrier, and even causing septic shock, sepsis, and sepsis.

[0003] In modern clinical treatment methods, surgery is one of the important treatment methods, and medical dressings are also the top priority of this treatment method. Medical dressings are supplies for dressing wounds, medical materials used to cover sores, wounds or other damages, which have the ability to prevent further infection of wounds and provide a more favorable microbial environment for wound healing. Currently, the commonly used medical dressings are mainly gauze, bandages, etc. Although the above-mentioned medical dressings can protect wounds to a certain extent, are simple to make and have low cost, they absorb less wound exudate, which is not conducive to wound healing, and will also increase the pain of patients when changing dressings. In this context, new wound dressings have attracted much attention in the medical field.

[0004] New wound dressings refer to dressing products with new materials, functions or performances that are innovatively designed and improved on the basis of traditional dressings. Such wound dressings have the functions of protecting wounds and healing wounds. Currently, there are many new wound dressings on the market, but most of them have problems such as poor wound healing performance and low absorption of wound exudate.

[0005] Therefore, it is of great significance to develop a medical dressing that can absorb exudate well and promote wound healing. Summary of the invention

[0006] The first purpose of the present invention is to provide a medical dressing for promoting surgical wound healing, which not only has good exudate absorption capacity but also can promote wound healing.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] A medical dressing for promoting surgical wound healing comprises the following raw materials in parts by weight: 5-10 parts of carboxymethyl chitosan, 0.5-1 part of vaccaria cyclopeptide B, 0.8-1.3 parts of sphaerocephala polysaccharide, 1-3 parts of modified montmorillonite, 0.5-1 part of carbomer, 1-5 parts of glycerol and 100-120 parts of water.

[0009] Furthermore, the following raw materials are included in parts by weight: 7 parts of carboxymethyl chitosan, 0.8 parts of Vaccaria cyclopeptide B, 1 part of Polysaccharide of Aquilegia multiflora, 2 parts of modified montmorillonite, 0.6 parts of Carbomer, 3 parts of glycerol, and 110 parts of water.

[0010] Furthermore, the preparation process of the modified montmorillonite is as follows:

[0011] The montmorillonite is added to an aqueous solution of a surfactant and zinc chloride for stirring reaction. After the reaction is completed, the modified montmorillonite is obtained by filtering, washing, drying and calcining.

[0012] Furthermore, the mass ratio of the montmorillonite, the surfactant and the zinc chloride is 1:(0.01-0.05):(0.05-0.1).

[0013] Furthermore, the surfactant is octadecyltrimethyl chloride.

[0014] Furthermore, the stirring reaction temperature is 85-95° C., and the stirring reaction time is 1.5-2.5 h.

[0015] Furthermore, the calcination temperature is 550-650° C., and the calcination time is 4-6 hours.

[0016] The second purpose of the present invention is to provide a method for preparing a medical dressing for promoting surgical wound healing, which has a simple preparation process and is easy to practice and produce.

[0017] In order to achieve the above object, the technical solution adopted by the present invention is:

[0018] The method for preparing the medical dressing for promoting surgical wound healing comprises the following steps:

[0019] The raw materials are uniformly mixed according to the weight ratio, and then cross-linked by radiation.

[0020] Furthermore, the radiation cross-linking time is 40-50 minutes.

[0021] The beneficial technical effects of the present invention are:

[0022] The medical dressing of the present invention contains ingredients such as Vaccaria cyclopeptide B, seaweed polysaccharide, and modified montmorillonite, and the synergistic effect of Vaccaria cyclopeptide B and seaweed polysaccharide can promote wound healing. In addition, octadecyltrimethyl chloride and zinc chloride are added to the montmorillonite, and zinc ions enter the montmorillonite interlayer through ion exchange reaction, and form stable zinc oxide after roasting, so that the interlayer distance of the montmorillonite is increased, which is more conducive to the diffusion and penetration of molecules or ions in the exudate, thereby improving the exudate absorption capacity of the medical dressing. DETAILED DESCRIPTION

[0023] The following is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or replacements can also be made, all of which should be deemed to belong to the protection scope of the present invention. The specific conditions not indicated in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0024] Example 1

[0025] A medical dressing for promoting surgical wound healing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 0.8 parts of vaccaria cyclopeptide B, 1 part of kelp polysaccharide, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0026] Among them, the preparation process of modified montmorillonite is as follows:

[0027] According to the mass ratio of montmorillonite, octadecyltrimethyl chloride and zinc chloride of 1:0.04:0.07, montmorillonite was added to an aqueous solution of octadecyltrimethyl chloride and zinc chloride, and stirred for reaction at 90°C for 2 hours. After the reaction was completed, it was filtered, washed and dried, and then calcined at 600°C for 5 hours to obtain modified montmorillonite.

[0028] The preparation process of the polysaccharide of Haihaicai is as follows:

[0029] The powder of the crushed seaweed is added to 25 times the amount of water and soaked for 30 hours. Then the mixed solution is placed in a centrifuge at a speed of 4500rpm and centrifuged for 15 minutes to obtain a supernatant. The supernatant is concentrated to 10% of the original volume, and 3 times the amount of 95% ethanol is added and allowed to stand for 12 hours. Then the mixed solution is transferred to a centrifuge at a speed of 4500rpm and centrifuged for 15 minutes, and the supernatant is discarded to obtain a crude polysaccharide of seaweed. Water and Sevage reagent are added to the crude polysaccharide of seaweed to deproteinize, and the mixture is transferred to a centrifuge at a speed of 4500rpm and centrifuged for 15 minutes. This is repeated 3 times. After reduced pressure concentration and freeze-drying, a polysaccharide of seaweed is obtained.

[0030] The preparation process of the above-mentioned medical dressing for promoting surgical wound healing is as follows:

[0031] According to the above weight ratio, mix the raw materials evenly and cross-link them under ultraviolet light for 45 minutes.

[0032] Example 2

[0033] A medical dressing for promoting surgical wound healing is composed of the following raw materials in parts by weight: 5 parts of carboxymethyl chitosan, 0.5 parts of fenugreek cyclopeptide B, 0.8 parts of scutellaria baicalensis polysaccharide, 1 part of modified montmorillonite, 0.5 parts of carbomer, 1 part of glycerol and 100 parts of water.

[0034] Among them, the preparation process of modified montmorillonite is as follows:

[0035] According to the mass ratio of montmorillonite, octadecyltrimethyl chloride and zinc chloride of 1:0.01:0.05, montmorillonite was added to the aqueous solution of octadecyltrimethyl chloride and zinc chloride, and stirred for reaction at 85°C for 2.5 hours. After the reaction was completed, it was filtered, washed and dried, and then calcined at 550°C for 6 hours to obtain modified montmorillonite.

[0036] The preparation process of the polysaccharide of Aquilegia salsa is the same as that in Example 1.

[0037] The preparation process of the above-mentioned medical dressing for promoting surgical wound healing is as follows:

[0038] According to the above weight ratio, mix the raw materials evenly and cross-link them under ultraviolet light for 40 minutes.

[0039] Example 3

[0040] A medical dressing for promoting surgical wound healing is composed of the following raw materials in parts by weight: 10 parts of carboxymethyl chitosan, 1 part of vaccaria cyclopeptide B, 1.3 parts of kelp polysaccharide, 3 parts of modified montmorillonite, 1 part of carbomer, 5 parts of glycerol and 120 parts of water.

[0041] Among them, the preparation process of modified montmorillonite is as follows:

[0042] According to the mass ratio of montmorillonite, octadecyltrimethyl chloride and zinc chloride of 1:0.05:0.1, montmorillonite was added to the aqueous solution of octadecyltrimethyl chloride and zinc chloride, and stirred for reaction at 95°C for 1.5 hours. After the reaction was completed, it was filtered, washed, dried, and then calcined at 650°C for 4 hours to obtain modified montmorillonite.

[0043] The preparation process of the polysaccharide of Aquilegia salsa is the same as that in Example 1.

[0044] The preparation process of the above-mentioned medical dressing for promoting surgical wound healing is as follows:

[0045] According to the above weight ratio, mix the raw materials evenly and cross-link them under ultraviolet light for 50 minutes.

[0046] Comparative Example 1

[0047] The difference between Comparative Example 1 and Example 1 is only that:

[0048] The medical dressing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 1 part of sea hail polysaccharide, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0049] Comparative Example 2

[0050] The difference between Comparative Example 2 and Example 1 is only that:

[0051] The medical dressing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 1.8 parts of sea hail polysaccharide, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0052] Comparative Example 3

[0053] The difference between Comparative Example 3 and Example 1 is only that:

[0054] The medical dressing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 0.8 parts of fenugreek cyclopeptide B, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0055] Comparative Example 4

[0056] The difference between Comparative Example 4 and Example 1 is only that:

[0057] The medical dressing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 1.8 parts of fenugreek cyclopeptide B, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0058] Comparative Example 5

[0059] The difference between Comparative Example 5 and Example 1 is only that:

[0060] The medical dressing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0061] Comparative Example 6

[0062] The difference between Comparative Example 6 and Example 1 is only that:

[0063] The medical dressing is composed of the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 0.8 parts of Vaccaria cyclopeptide B, 1 part of Psoralea corylifolia polysaccharide, 2 parts of montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

[0064] Test Example 1

[0065] Test Example 1 The wound healing performance of the medical dressings obtained in Examples 1-3 and Comparative Examples 1-6 was tested, and the specific process was as follows:

[0066] Experimental subjects and their groups: A total of 90 adult healthy SD rats were randomly divided into 9 groups, with a male-female ratio of 1:1 in each group.

[0067] Wound formation: Each rat was anesthetized by intraperitoneal injection of 30 mg / kg 10% chloral hydrate, and then the back was shaved to expose the skin. A circular wound was formed with a 10 mm diameter trephine burr with the back thoracic segment as the center.

[0068] Bandaging: The medical dressings obtained in Examples 1-3 and Comparative Examples 1-6 were used for wound bandaging, and the medical dressings were changed every 2 days. The appearance of the wound of each rat was observed and recorded. The length and width of the wound of each group of rats were measured on the 7th and 14th days after surgery, and the wound area and wound healing rate were calculated. The calculation formula of the wound healing rate is as follows:

[0069] Wound healing rate = (initial wound area - wound area measured on the nth day) / initial wound area × 100%. See Table 1 for the results.

[0070] Table 1

[0071]

[0072] As can be seen from Table 1, the medical dressings obtained in Examples 1-3 can promote wound healing. Compared with Examples 1-3, the wound healing performance of the medical dressings obtained in Comparative Examples 1-5 is significantly reduced, and the wound healing performance of the medical dressings obtained in Comparative Example 6 is slightly reduced. Further analysis shows that the medical dressing obtained in Comparative Example 1 omits the raw material Vaccaria cyclotide B; the medical dressing obtained in Comparative Example 2 omits the raw material Vaccaria cyclotide B, but increases the amount of the raw material Scutellaria polysaccharide; the medical dressing obtained in Comparative Example 3 omits the raw material Scutellaria polysaccharide; the medical dressing obtained in Comparative Example 4 omits the raw material Scutellaria polysaccharide, but increases the amount of the raw material Vaccaria cyclotide B; the medical dressing obtained in Comparative Example 5 omits both the raw materials Scutellaria polysaccharide and Vaccaria cyclotide B. The above test results show that the synergistic effect of Scutellaria polysaccharide and Vaccaria cyclotide B can promote wound healing.

[0073] Test Example 2

[0074] Test Example 2 The exudate absorption capacity of the medical dressings obtained in Examples 1-3 and Comparative Examples 1-6 was tested, and the specific process was as follows:

[0075] According to YY / T0471.1-2004 “Test methods for contact wound dressings Part 1: Liquid absorbency”, the exudate absorption performance test was carried out. The results are shown in Table 2.

[0076] Table 2

[0077]

[0078] As can be seen from Table 2, the medical dressings obtained in Examples 1-3 have good exudate absorption capacity. The exudate absorption capacity of the medical dressings obtained in Comparative Examples 1-5 is not significantly different from that of Examples 1-3. Compared with Examples 1-3, the exudate absorption capacity of the medical dressing obtained in Comparative Example 6 is significantly reduced. The medical dressing obtained in Comparative Example 6 uses montmorillonite instead of the modified montmorillonite of the present invention. The above test results show that modified montmorillonite can improve the exudate absorption capacity of medical dressings.

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A medical dressing for promoting surgical wound healing, characterized in that: The invention comprises the following raw materials in parts by weight: 5-10 parts of carboxymethyl chitosan, 0.5-1 parts of Vaccaria cyclopeptide B, 0.8-1.3 parts of sphaerocephala polysaccharide, 1-3 parts of modified montmorillonite, 0.5-1 parts of carbomer, 1-5 parts of glycerol and 100-120 parts of water; The preparation process of the polysaccharide of Psoralea corylifolia is as follows: The powder of the crushed seaweed was added to 25 times the amount of water, soaked for 30 hours, and then the mixed solution was placed in a centrifuge at a speed of 4500rpm for 15 minutes to obtain a supernatant, and the supernatant was concentrated to 10% of the original volume, and then 3 times the amount of 95% ethanol was added and allowed to stand for 12 hours; then the mixed solution was transferred to a centrifuge at a speed of 4500rpm for 15 minutes, and the supernatant was discarded to obtain a crude polysaccharide of seaweed; water and Sevage reagent were added to the crude polysaccharide of seaweed for deproteinization, and the mixture was transferred to a centrifuge at a speed of 4500rpm for 15 minutes, and the mixture was repeated 3 times, and the mixture was concentrated under reduced pressure and freeze-dried to obtain the crude polysaccharide; The preparation process of the modified montmorillonite is as follows: The montmorillonite is added to an aqueous solution of a surfactant and zinc chloride for stirring reaction. After the reaction is completed, the modified montmorillonite is obtained by filtering, washing, drying and calcining.

2. The medical dressing for promoting surgical wound healing as claimed in claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 7 parts of carboxymethyl chitosan, 0.8 parts of Vaccaria cyclopeptide B, 1 part of Psoralea corylifolia polysaccharide, 2 parts of modified montmorillonite, 0.6 parts of carbomer, 3 parts of glycerol and 110 parts of water.

3. The medical dressing for promoting surgical wound healing according to claim 1, characterized in that: The mass ratio of the montmorillonite, the surfactant and the zinc chloride is 1: (0.01-0.05): (0.05-0.1).

4. The medical dressing for promoting surgical wound healing according to claim 1, characterized in that: The surfactant is octadecyltrimethyl chloride.

5. The medical dressing for promoting surgical wound healing according to claim 1, characterized in that: The stirring reaction temperature is 85-95° C., and the stirring reaction time is 1.5-2.5 h.

6. The medical dressing for promoting surgical wound healing according to claim 1, characterized in that: The calcination temperature is 550-650° C., and the calcination time is 4-6 hours.

7. The method for preparing the medical dressing for promoting surgical wound healing according to claim 1, characterized in that: The following steps are involved: The raw materials are uniformly mixed according to the weight ratio, and then cross-linked by radiation.

8. The method for preparing the medical dressing for promoting surgical wound healing as claimed in claim 7, characterized in that: The time of the radiation cross-linking is 40-50 minutes.

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