Screening method of activated carbon for production of Shenqi body resistance strengthening injection

Abstract

The invention relates to a screening method of activated carbon for production of Shenqi body resistance strengthening injection, which comprises the following steps: carrying out mid-infrared spectrum detection on the activated carbon to obtain transmittance under a preset wave number; preprocessing the transmittance under the preset wave number to obtain a corrected transmittance under the preset wave number; according to a calculation formula, a predicted value of each index component amount of the unit activated carbon adsorption Shenqi strengthening injection is calculated through the transmittance and the corrected transmittance under a preset wave number, and a preset threshold value of each index component amount of the unit activated carbon adsorption Shenqi strengthening injection is set; and comparing the predicted value with a preset threshold value, and screening the activated carbon for production of the Shenqi body resistance strengthening injection according to a comparison result. The technology for quantitatively predicting the equilibrium adsorption capacity of the activated carbon to the index components based on the mid-infrared spectrum is established for the Shenqi body resistance strengthening system, the cost is low, the speed is higher, the method is greener, the prediction result is more accurate, and a new method can be provided for screening the activated carbon for industrial production of the Shenqi body resistance strengthening injection.

Description

Technical Field

[0001] The present application relates to the field of medical technology, and in particular to a method for screening activated carbon for the production of Shenqi Fuzheng Injection. Background Art

[0002] Activated carbon has a strong adsorption capacity for pyrogens, and also has the functions of decolorization and filtration. Therefore, it is widely used in the production of injections to remove pyrogens and decolorize. Shenqi Fuzheng Injection is a national Class II new Chinese medicine launched by Limin Pharmaceutical Factory of Livzon Group in 1999. This product is made of traditional Chinese medicine Codonopsis pilosula and Astragalus membranaceus as raw materials, and is processed by water extraction and alcohol precipitation. It is a clear large infusion of Chinese medicine, which has the effect of invigorating qi and strengthening the body, and is mainly used to treat tumor diseases.

[0003] At present, in the actual production process of this product, due to the relatively extensive production process of activated carbon and the lack of objective quantitative standards, there is a problem of large quality differences between batches. In this case, activated carbon adsorption will cause fluctuations in the content of active ingredients in Shenqi Fuzheng Injection, which is not conducive to product quality control. Improper use will lead to unqualified products. Therefore, the company urgently needs an activated carbon characterization method to establish a quantitative method for the relevant properties of activated carbon to formulate quality control standards and indicate the selection of activated carbon, thereby solving the problem of large quality differences between activated carbon batches. Summary of the invention

[0004] Based on this, it is necessary to provide a screening method for activated carbon used in the production of Shenqi Fuzheng Injection, which has relatively accurate prediction results and can provide more effective activated carbon quantitative quality control standards, thereby indicating the selection of activated carbon to solve the problem of large batch differences.

[0005] The technical solution of this application is as follows:

[0006] The present invention provides a method for screening activated carbon for the production of Shenqi Fuzheng injection, comprising the following steps:

[0007] Perform mid-infrared spectroscopy on the activated carbon to obtain the transmittance at a preset wave number;

[0008] Preprocessing the transmittance at the preset wavenumber to obtain a corrected transmittance at the preset wavenumber;

[0009] According to the calculation formula, the predicted value of the amount of each index component of Shenqi Fuzheng Injection adsorbed by unit activated carbon is calculated by the transmittance and corrected transmittance at the preset wave number, and the preset threshold value of the amount of each index component of Shenqi Fuzheng Injection adsorbed by unit activated carbon is set;

[0010] Compare the predicted value with the preset threshold value, and select the activated carbon for the production of Shenqi Fuzheng Injection according to the comparison result;

[0011] The index components are astragaloside, adenine, adenosine and astragaloside IV.

[0012] In one embodiment, the preset wave number is a specific wave number of activated carbon in the mid-infrared spectrum;

[0013] Optionally, the preset wave number is 1560±5cm -1 、2325±5cm -1 、3050±5cm -1 and 3445±5cm -1 .

[0014] In one embodiment, the step of preprocessing the transmittance at the preset wave number includes: subtracting 4000±5cm from the transmittance at the preset wave number. -1 The transmittance under the preset wave number is obtained.

[0015] In one embodiment, the calculation formula for the predicted value of the amount of isoflavone glycosides adsorbed by unit activated carbon is shown in the following formula (1):

[0016]

[0017] in, Represents the predicted value of the amount of isoflavone glycosides adsorbed by activated carbon per unit (mg / g), Represents 1560±5cm -1 Corrected transmittance (%) under .

[0018] In one embodiment, the calculation formula for the predicted value of the amount of adenine adsorbed by a unit of activated carbon is shown in the following formula (2):

[0019]

[0020] in, represents the predicted value of adenine adsorption per unit activated carbon (mg / g), Represents 1560±5cm -1 The transmittance under %.

[0021] In one embodiment, the calculation formula for the predicted value of the amount of adenosine adsorbed per unit activated carbon is shown in the following formula (3):

[0022]

[0023] in, represents the predicted value of adenosine adsorption per unit activated carbon (mg / g), Represents 1560±5cm -1 Transmittance under (%), Represents 2325±5cm-1 Corrected transmittance (%) under .

[0024] In one embodiment, the calculation formula for the predicted value of the amount of astragaloside IV adsorbed by unit activated carbon is shown in the following formula (4):

[0025]

[0026] in, Represents the predicted value of astragaloside IV adsorbed per unit activated carbon (mg / g), and Represents 3050±5cm respectively -1 and 3442±5cm -1 Corrected transmittance (%) under .

[0027] In one embodiment, the step of screening activated carbon for the production of Shenqi Fuzheng Injection according to the comparison results comprises:

[0028] The predicted value is compared with the preset threshold value, and the activated carbon whose predicted values ​​are all within the preset threshold value range is selected as the activated carbon for the production of Shenqi Fuzheng Injection.

[0029] In one embodiment, the preset threshold value of the amount of calycosin adsorbed per unit activated carbon is 20 mg / g to 60 mg / g; and / or

[0030] The preset threshold value of adenine adsorption per unit activated carbon is 5 mg / g~30 mg / g.

[0031] In one embodiment, the preset threshold value of the amount of adenosine adsorbed per unit of activated carbon is 10 mg / g to 40 mg / g; and / or

[0032] The preset threshold value of astragaloside IV adsorbed by unit activated carbon is 50mg / g~165mg / g.

[0033] Compared with the prior art, this application has the following beneficial effects:

[0034] This application establishes a technology for quantitatively predicting the equilibrium adsorption amount of activated carbon for index components based on mid-infrared spectroscopy for the Shenqi Fuzheng system. It has the advantage of good prediction effect and provides a more effective quantitative quality standard for activated carbon, thereby indicating the selection of activated carbon and solving the problem of large batch differences. At the same time, compared with directly using activated carbon for adsorption when producing Shenqi Fuzheng injection, the technical solution of this application has lower cost, faster speed, and is more green, and the prediction results are more accurate, which can provide a new method for the screening of activated carbon for the industrial production of Shenqi Fuzheng injection. DETAILED DESCRIPTION

[0035] The present application will be further described in detail below in conjunction with specific embodiments. The present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thoroughly understood.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0037] Unless otherwise specified or incompatible herewith, the terms and phrases used herein shall have the following meanings:

[0038] The percentage content involved in the present invention, unless otherwise specified, refers to mass percentage for solid-liquid mixing and solid-solid mixing, and refers to volume percentage for liquid-liquid mixing.

[0039] As used herein, "one or more" refers to any one, any two, or any two or more of the listed items.

[0040] Herein, the optional scope of "and / or", "or / and", and "and / or" includes any one of two or more relevant listed items, and also includes any and all combinations of the relevant listed items, wherein said any and all combinations include any two relevant listed items, any more relevant listed items, or a combination of all relevant listed items. (It should be noted that when at least three items are connected by at least two conjunctions selected from "and / or", "or / and", and "and / or", it should be understood that in the present application, the technical solution undoubtedly includes technical solutions that are all connected by "logical AND", and also undoubtedly includes technical solutions that are all connected by "logical OR". For example, "A and / or B" includes three parallel solutions of A, B and A+B. For another example, the technical solution of "A, and / or, B, and / or, C, and / or, D" includes any one of A, B, C, and D (that is, technical solutions that are all connected by "logical OR"), and also includes any and all combinations of A, B, C, and D, that is, a combination of any two or any three of A, B, C, and D, and also includes a combination of four items of A, B, C, and D (that is, a technical solution that is all connected by "logical AND").)

[0041] Herein, "further", "further", "particularly" and the like are used for descriptive purposes to indicate differences in content, but should not be construed as limiting the scope of protection of this application.

[0042] Herein, "first aspect", "second aspect", "third aspect", "fourth aspect", etc. are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or quantity, nor can they be understood as implicitly indicating the importance or quantity of the indicated technical features. In addition, "first", "second", "third", "fourth", etc. only serve the purpose of non-exhaustive enumeration and description, and it should be understood that they do not constitute a closed limitation on quantity. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined. In the description of the present application, the meaning of "several" is at least one, such as one, two, etc., unless otherwise clearly and specifically defined.

[0043] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0044] Specifically, only some numerical ranges are specifically disclosed herein. However, any lower limit can be combined with any upper limit to form an undefined range; and any lower limit can be combined with other lower limits to form an undefined range, and any upper limit can be combined with any other upper limit to form an undefined range. In addition, each individually disclosed point or single value itself can be combined with any other point or single value as a lower limit or upper limit or with other lower limits or upper limits to form an undefined range. The use of numerical ranges represented by endpoints includes all numbers within the range and any range within the range, for example, 1 to 5 includes 1, 1.1, 1.3, 1.5, 2, 2.75, 3, 3.80, 4 and 5, etc.

[0045] The percentage contents involved in this application, unless otherwise specified, refer to mass percentage for solid-liquid mixing and solid-solid mixing, and refer to volume percentage for liquid-liquid mixing.

[0046] The percentage concentrations mentioned in this application, unless otherwise specified, refer to the final concentration, which refers to the percentage of the added component in the system after the addition of the component.

[0047] The temperature parameters in this application, unless otherwise specified, allow both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range controlled by the instrument. Fluctuations within the range of ±5°C, ±2°C, ±1°C, ±0.5°C, ±0.4°C, ±0.3°C, ±0.2°C, and ±0.1°C are allowed. Normal temperature or room temperature in this application refers to no temperature control operation, generally 4°C~35°C, preferably 20±5°C.

[0048] There are three main ways to characterize activated carbon reported so far: The first is that the conventional indicators of activated carbon mainly include iodine adsorption value, methylene blue adsorption value, phenol adsorption value, etc., and the detection method is carried out in accordance with the corresponding national standards. The second method uses nitrogen isothermal adsorption method, and uses specific surface area and porosity analyzer to detect the specific surface area, pore volume, average pore size and other pore structure parameters of activated carbon. The third method uses Fourier transform infrared spectrometer to detect the mid-infrared spectrum of activated carbon, and the test band is 400~4000 cm -1 Although there are many reports on the application of activated carbon in traditional Chinese medicine injections and characterization methods of related structures, there are few reports on the establishment of a quantitative relationship between the relevant characterization information of activated carbon and its adsorption performance. Moreover, most of the relevant characterization information is not strongly correlated with the adsorption amount of the index components of the ginseng and astragalus tonic system, so a new method is needed to establish the relationship between the characterization information of activated carbon and the adsorption amount of the index components of the ginseng and astragalus tonic system, so as to screen activated carbon based on this relationship.

[0049] The characterization method of mid-infrared spectroscopy has the advantages of being green, low cost, and fast detection speed. Based on this, the technical personnel of this application have found through extensive research that the surface chemical properties of activated carbon can be characterized by measuring the mid-infrared spectrum of activated carbon. After preprocessing the spectral data, the transmittance and corrected transmittance at a specific wave number can be used to calculate the predicted value of the index component amount of the activated carbon adsorbed ginseng and Astragalus tonic system according to the calculation formula of different index components. At the same time, the method for quantitatively predicting the amount of index components of the activated carbon adsorbed ginseng and Astragalus tonic system based on mid-infrared spectroscopy established in this application has the advantage of good prediction effect, provides a quantitative quality standard for activated carbon, thereby indicating the selection of activated carbon and solving the problem of large differences between batches.

[0050] The present invention provides a method for screening activated carbon for the production of Shenqi Fuzheng injection, comprising the following steps:

[0051] Perform mid-infrared spectroscopy on the activated carbon to obtain the transmittance at a preset wave number;

[0052] Preprocessing the transmittance at the preset wavenumber to obtain a corrected transmittance at the preset wavenumber;

[0053] According to the calculation formula, the predicted value of the amount of each index component of Shenqi Fuzheng Injection adsorbed by unit activated carbon is calculated by the transmittance and corrected transmittance at the preset wave number, and the preset threshold value of the amount of each index component of Shenqi Fuzheng Injection adsorbed by unit activated carbon is set;

[0054] Compare the predicted value with the preset threshold value, and select the activated carbon for the production of Shenqi Fuzheng Injection according to the comparison result;

[0055] The index components are astragaloside, adenine, adenosine and astragaloside IV.

[0056] In some of these examples, the preset wavenumber is a specific wavenumber of the activated carbon mid-infrared spectrum.

[0057] In some examples, the preset wave number is 1560±5cm -1 、2325±5cm -1 、3050±5cm -1 and 3442±5cm -1 It is understandable that the preset wave number includes but is not limited to 1555cm -1 、1560cm -1 、1565cm -1 、2320cm -1 、2325cm -1 、2330cm -1 、3045cm -1 、3050cm -1 、3055cm -1 、3437cm -1 、3442cm -1 and 3447cm -1 Furthermore, the preset wave number is 1560cm -1 、2325cm -1 、3050cm -1 and 3442cm -1 .

[0058] In some examples, the step of preprocessing the transmittance at the preset wave number includes: subtracting 4000±5cm from the transmittance at the preset wave number. -1 The transmittance at the preset wave number is obtained by calculating the transmittance at the preset wave number to obtain the corrected transmittance at the preset wave number. Further, the step of pre-processing the transmittance at the preset wave number includes: subtracting 4000cm from the transmittance at the preset wave number. -1 The transmittance under the preset wave number is obtained.

[0059] In some of the examples, the calculation formula for the predicted value of the amount of isoflavone glycosides adsorbed by unit activated carbon is shown in the following formula (1):

[0060]

[0061] in, Represents the predicted value of the amount of isoflavone glycosides adsorbed by activated carbon per unit (mg / g), Represents 1560±5cm -1 Corrected transmittance (%) under .

[0062] In some of these examples, the calculation formula for the predicted value of the amount of adenine adsorbed per unit activated carbon is shown in the following formula (2):

[0063]

[0064] in, represents the predicted value of adenine adsorption per unit activated carbon (mg / g), Represents 1560±5cm -1 The transmittance under %.

[0065] In some of these examples, the calculation formula for the predicted value of the amount of adenosine adsorbed per unit activated carbon is shown in the following formula (3):

[0066]

[0067] in, represents the predicted value of adenosine adsorption per unit activated carbon (mg / g), Represents 1560±5cm -1 Transmittance under (%), Represents 2325±5cm -1 Corrected transmittance (%) under .

[0068] In some of the examples, the calculation formula for the predicted value of the amount of astragaloside IV adsorbed by unit activated carbon is shown in the following formula (4):

[0069]

[0070] in, Represents the predicted value of astragaloside IV adsorbed per unit activated carbon (mg / g), and Represents 3050±5cm respectively -1 and 3442±5cm -1 Corrected transmittance (%) under .

[0071] Specifically, the index component amount refers to the mass of the index component adsorbed by the unit activated carbon in equilibrium. The spectral data preprocessing, the selection of specific wavenumbers and the index component amount calculation formula of the present application are technically innovative, and can effectively use mid-infrared spectral data to predict the amount of index components of the activated carbon adsorbed ginseng and astragalus tonic system, thereby indicating the selection of activated carbon.

[0072] In some of these examples, the steps for screening activated carbon for the production of Shenqi Fuzheng Injection based on the comparison results include:

[0073] The predicted value is compared with the preset threshold value, and the activated carbon whose predicted values ​​are all within the preset threshold value range is selected as the activated carbon for the production of Shenqi Fuzheng Injection.

[0074] In some of the examples, the preset threshold of the amount of calycosin adsorbed per unit activated carbon is 20 mg / g to 60 mg / g. It is understandable that the preset threshold of the amount of calycosin adsorbed per unit activated carbon includes but is not limited to 20 mg / g, 30 mg / g, 40 mg / g, 50 mg / g, and 60 mg / g.

[0075] In some of the examples, the preset threshold value of the amount of adenine adsorbed by a unit of activated carbon is 5 mg / g to 30 mg / g. It is understandable that the preset threshold value of the amount of adenine adsorbed by a unit of activated carbon includes but is not limited to 5 mg / g, 10 mg / g, 15 mg / g, 20 mg / g, 25 mg / g, and 30 mg / g.

[0076] In some of the examples, the preset threshold of the amount of adenosine adsorbed per unit of activated carbon is 10 mg / g to 40 mg / g. It is understandable that the preset threshold of the amount of adenosine adsorbed per unit of activated carbon includes but is not limited to 10 mg / g, 20 mg / g, 30 mg / g, and 40 mg / g.

[0077] In some of the examples, the preset threshold of the amount of astragaloside IV adsorbed per unit activated carbon is 50 mg / g to 165 mg / g. It is understandable that the preset threshold of the amount of astragaloside IV adsorbed per unit activated carbon includes but is not limited to 50 mg / g, 70 mg / g, 90 mg / g, 110 mg / g, 130 mg / g, 150 mg / g, and 165 mg / g.

[0078] The following is further described in conjunction with specific embodiments. The raw materials involved in the following specific embodiments, unless otherwise specified, can all be commercially available; the instruments used, unless otherwise specified, can all be commercially available; the processes involved, unless otherwise specified, are all routinely selected by those skilled in the art.

[0079] A mid-infrared spectrometer (NICOLET iS50FT-IR, Thermo Scientific) was used to collect the mid-infrared spectra of the samples. The spectrum collection parameters were as follows: the spectrum collection range was 4000~400cm -1 , resolution 4cm -1 Each spectrum was scanned 32 times / s, each sample was sampled three times in parallel, and each pressed sheet was scanned twice.

[0080] The calculation formula used in Example 1-3 to calculate the predicted value of the amount of the index component of the ginseng and astragalus tonic system adsorbed by unit activated carbon is: calycosin isoflavone glycosides as formula (1), adenine as formula (2), adenosine as formula (3), and astragaloside IV as formula (4), as follows:

[0081]

[0082]

[0083]

[0084]

[0085] in , , and They represent the predicted values ​​of the amount of isoflavone glycosides, adenine, adenosine and astragaloside IV adsorbed by unit activated carbon (mg / g), Represents 1560cm -1 Transmittance under (%), , , and Represents 1560cm -1 、2325cm -1 、3050cm -1 、3442cm -1 Corrected transmittance (%) under .

[0086] The method for obtaining the experimental values ​​of the amount of each index component in the activated carbon in Examples 1-3 is: take the activated carbon and conduct an adsorption experiment according to the small-scale adsorption process of Shenqi Fuzheng Injection, take samples at intervals to detect the content of the index component, and fit the kinetic equation according to the detected value to obtain the experimental values ​​of the amount of each index component. The details are as follows:

[0087] Take 25 mL of ginseng and Astragalus extract, dilute it 20 times with purified water, mix well, and obtain the ginseng and Astragalus tonic dilution solution.

[0088] Take 0.107g of activated carbon, add it to the Shenqi Fuzheng dilution, stir at room temperature, the stirring speed is 250r / min, sample is taken every ten minutes, the sample is filtered through a 0.22µm filter membrane, and the sample is collected for the detection of the index component content.

[0089] The content detection methods of each indicator component are as follows:

[0090] (1) Detection methods of adenine, adenosine and calycosin isoflavone glycosides

[0091] Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel was used as filler (Waters Atlantis T3, 100 mm×2.1 mm, 3 μm); 0.05% formic acid aqueous solution was used as mobile phase A, and acetonitrile was used as mobile phase B; the elution gradient was shown in Table 1; the detection wavelength was 260 nm; the column temperature was 30°C; and the flow rate was 0.25 mL / min.

[0092] Table 1 Mobile phase elution gradient

[0093]

[0094] Preparation of test solution:

[0095] Diluted solution of Ginseng and Astragalus (before activated carbon adsorption): Take appropriate amount of the test sample and filter to obtain the solution.

[0096] Diluted solution of Ginseng and Astragalus (after adsorption on activated carbon): Take an appropriate amount of the test sample and filter it to obtain the solution.

[0097] Determination method: Accurately pipette 5 μL of the test solution respectively, inject into the liquid chromatograph and determine.

[0098] (2) Astragaloside IV detection method

[0099] Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel was used as the filler (chromatographic column ZORBAX SB-C18 250mm×4.6mm, 5μm); the mobile phase was 0.2% formic acid-water (A) and acetonitrile (B); the column temperature was 30℃; the flow rate was 0.8mL / min, the ELSD nebulization temperature was 30℃; the drift tube temperature was 80℃; the nitrogen flow rate was 1.6L / min, and the evaporative light scattering detector was used for detection, and the gradient elution was performed according to the provisions in Table 2.

[0100] Table 2 Mobile phase elution gradient

[0101]

[0102] Preparation of the test solution: Activate the solid phase extraction column, that is, treat the C18 solid phase extraction column with 12mL of methanol and 12mL of purified water in turn. Take 12mL of activated carbon adsorption liquid, add 0.5mL of ammonia water, centrifuge and mix well, and set aside. Add the activated carbon adsorption liquid to the activated solid phase extraction column and adsorb for 30 minutes. Then wash with water for 3 column volumes, and then elute with methanol until the eluent color no longer changes, and collect the methanol eluent. Evaporate the methanol eluent to dryness in a water bath, add 1mL of methanol to dissolve, and you have it.

[0103] Determination method: Accurately pipette 10 μL of standard solution and test solution respectively, inject into liquid chromatograph and determine.

[0104] Specifically, the kinetic equation used is the pseudo-first-order kinetic equation, that is, equation (1):

[0105]

[0106] Where k1 is the pseudo-first-order adsorption rate constant (min -1 ), q e is the activated carbon adsorption capacity at equilibrium time (mg / g), q t is the amount of activated carbon adsorption at time t (mg / g). The pseudo-first-order kinetic equation is used to perform nonlinear fitting on the activated carbon adsorption experimental data of the index component and calculate the kinetic parameter q e , which is the experimental value of the component quantity of this indicator.

[0107] The following are specific embodiments.

[0108] Example 1

[0109] Take an appropriate amount of activated carbon with batch number 230705 provided by Limin Pharmaceutical Factory of Livzon Group, mix and press the activated carbon sample with spectrally pure dry KBr (potassium bromide) powder at a mass ratio of 1:100, and then send it to the mid-infrared spectrometer for detection. The test band is 400-4000cm -1 . Test the transmittance at a specific wave number and then subtract 4000cm -1 The transmittance and corrected transmittance at specific wave numbers are shown in Table 3.

[0110] Table 3 Transmittance and corrected transmittance of batch number 230705 at specific wave numbers

[0111]

[0112] The index component amounts of the activated carbon adsorbed ginseng and Astragalus tonic system were calculated based on the transmittance and corrected transmittance at specific wave numbers. The predicted results of the amounts of each index component and the experimental results are shown in Table 4.

[0113] Table 4 Prediction and experimental results of each indicator component of batch number 230705

[0114]

[0115] From the relative deviation of each index component, except for the poor prediction of adenine, the other index components all showed good prediction effects. According to the proposed activated carbon optimization method, the amount of calycosin glycosides in this batch of activated carbon exceeded the threshold range (>60 mg / g), so this batch of activated carbon was not selected for adsorption.

[0116] Example 2

[0117] Take an appropriate amount of activated carbon with batch number 230706 provided by Limin Pharmaceutical Factory of Livzon Group, mix and press the activated carbon sample with spectrally pure dry KBr powder at a mass ratio of 1:100, and then send it to the mid-infrared spectrometer for detection. The test band is 400-4000cm -1 . Test the transmittance at a specific wave number and then subtract 4000cm -1 The transmittance and corrected transmittance at specific wave numbers are shown in Table 5.

[0118] Table 5 Transmittance and corrected transmittance of batch number 230706 at specific wave numbers

[0119]

[0120] The index component amounts of the activated carbon adsorbed ginseng and Astragalus tonic system were calculated based on the transmittance and corrected transmittance at specific wave numbers. The predicted results of the amounts of each index component and the experimental results are shown in Table 6.

[0121] Table 6 Prediction and experimental results of each indicator component of batch number 230706

[0122]

[0123] From the relative deviation of each index component, all index components showed good prediction effect, and the highest relative deviation was below 5%. According to the proposed activated carbon optimization method, the amount of each index component of this batch of activated carbon was within the preset threshold, so this batch of activated carbon was selected for adsorption.

[0124] Example 3

[0125] Take an appropriate amount of activated carbon with batch number 230807 provided by Limin Pharmaceutical Factory of Livzon Group, mix and press the activated carbon sample with spectrally pure dry KBr powder at a mass ratio of 1:100, and then send it to the mid-infrared spectrometer for detection. The test band is 400-4000cm -1 . Test the transmittance at a specific wave number and then subtract 4000cm-1 The transmittance and corrected transmittance at specific wave numbers are shown in Table 7.

[0126] Table 7 Transmittance and corrected transmittance of batch number 230807 at specific wave numbers

[0127]

[0128] The index component amounts of the activated carbon adsorbed ginseng and Astragalus tonic system were calculated based on the transmittance and corrected transmittance at specific wave numbers. The predicted results of the amounts of each index component and the experimental results are shown in Table 8.

[0129] Table 8 Prediction and experimental results of each indicator component of batch number 230807

[0130]

[0131] From the relative deviation of each index component, all index components showed good prediction effect, and the highest relative deviation was less than 5%. According to the proposed activated carbon optimization method, the astragaloside index of this batch of activated carbon exceeded the threshold range (>165 mg / g), so this batch of activated carbon was not selected for adsorption.

[0132] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0133] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims, and the description may be used to interpret the content of the claims.

Claims

1. A method for screening activated carbon for the production of Shenqi Fuzheng injection, characterized in that: The following steps are involved: Perform mid-infrared spectroscopy on the activated carbon to obtain the transmittance at a preset wave number; Preprocessing the transmittance at the preset wavenumber to obtain a corrected transmittance at the preset wavenumber; According to the calculation formula, the predicted value of the amount of each index component of Shenqi Fuzheng Injection adsorbed by unit activated carbon is calculated by the transmittance and corrected transmittance at the preset wave number, and the preset threshold value of the amount of each index component of Shenqi Fuzheng Injection adsorbed by unit activated carbon is set; Compare the predicted value with the preset threshold value, and select the activated carbon for the production of Shenqi Fuzheng Injection according to the comparison result; The index components are astragaloside, adenine, adenosine and astragaloside IV.

2. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 1, characterized in that: The preset wave number is a specific wave number of activated carbon in the mid-infrared spectrum; Optionally, the preset wave number is 1560±5cm -1 、2325±5cm -1 、3050±5cm -1 and 3442±5cm -1 .

3. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 2, characterized in that: The step of preprocessing the transmittance at the preset wave number includes: subtracting 4000±5cm from the transmittance at the preset wave number; -1 The transmittance under the preset wave number is obtained.

4. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 3, characterized in that: The calculation formula for the predicted value of the amount of isoflavone glycosides adsorbed by unit activated carbon is shown in the following formula (1): in, Represents the predicted value of the amount of isoflavone glycosides adsorbed by activated carbon per unit (mg / g), Represents 1560±5cm -1 Corrected transmittance (%) under .

5. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 3, characterized in that: The calculation formula for the predicted value of the amount of adenine adsorbed by unit activated carbon is shown in the following formula (2): in, represents the predicted value of adenine adsorption per unit activated carbon (mg / g), Represents 1560±5cm -1 The transmittance under %.

6. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 3, characterized in that: The calculation formula for the predicted value of the amount of adenosine adsorbed by unit activated carbon is shown in the following formula (3): in, Represents the predicted value of unit activated carbon adsorption capacity (mg / g), Represents 1560±5cm -1 Transmittance under (%), Represents 2325±5 cm -1 Corrected transmittance (%) under .

7. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 3, characterized in that: The calculation formula for the predicted value of the amount of astragaloside IV adsorbed by unit activated carbon is shown in the following formula (4): in, Represents the predicted value of astragaloside IV adsorbed per unit activated carbon (mg / g), and Respectively represent 3050±5cm -1 and 3442±5cm -1 Corrected transmittance (%) under .

8. The method for screening activated carbon for use in the production of Shenqi Fuzheng Injection according to any one of claims 1 to 7, characterized in that: The steps of screening activated carbon for the production of Shenqi Fuzheng Injection according to the comparison results include: The predicted value is compared with the preset threshold value, and the activated carbon whose predicted values ​​are all within the preset threshold value range is selected as the activated carbon for the production of Shenqi Fuzheng Injection.

9. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 8, characterized in that: The preset threshold value of the amount of calycosin adsorbed per unit activated carbon is 20 mg / g to 60 mg / g; and / or The preset threshold value of adenine adsorption per unit activated carbon is 5 mg / g~30 mg / g.

10. The method for screening activated carbon for the production of Shenqi Fuzheng Injection according to claim 8, characterized in that: The preset threshold of adenosine adsorption per unit activated carbon is 10mg / g~40mg / g; and / or The preset threshold value of astragaloside IV adsorbed by unit activated carbon is 50mg / g~165mg / g.

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